Tan0013338 (gene) Snake gourd v1

Overview
NameTan0013338
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionDiacylglycerol O-acyltransferase
LocationLG02: 1801911 .. 1806391 (+)
RNA-Seq ExpressionTan0013338
SyntenyTan0013338
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTATTGTTTCAATTTCCAACAGAATCTCCCTCATTTAGATCAAATAATTTGGGATCATCGGTAATGATCAAGCTTTTTTCTTCTTTTTATTATTATTATTATTTTTTCTTTCAAGCAAAGACAAAGAGCTACTTTTTCTTTAGAAATATAGAGTGAAAAAGTGATTAAATTAGATTCGTTTTTGAACTTTTTGCAGGCAGTGAAACATATATATATATATATTAGTAATGGAGATTTTGGAAGATTCAACAGCAACAACTCCCATGAGCCCTTTGAGCCAATACTTCAACACCTCTGAAATGTGTGTTTCTGTTTTGGGTGTGATTGAACTCGAATCTCCAATTACTCGCTACGAAGCCATGTCAATTATCACTGATGTTCTCATTCCTGCCAATCCACGTTTCACTTCCATCATGGTCCCTCTCTCACAATCTCATTTGGTTCTTAGTTTTTGATTTTGGAAAATTATACTTGTTTTCTCACTATTTTTTTTTCACCCTGAATTCTTAGAATTAGTTTTCAAAACTTGACTTGATTTTTAGACATCTAAAAAGTAGACGAGAAAATATAGAAAACTATAAGTAGAAATAGTATTTATACACTTAATTTTAAAAAACTAAAAGCTAGATAATAATTAAAATTACAGCCCTTAGGCTTTTGATCGTCCGATAAAGTAGGAAAGTGTTGCTTATGGAGCCAAACCTAGGTGATCTATCTATGATTTAATTTTTAAATTTAATGTTTTCTATAAGAGAGAGTAGCCAACATGAGCATAACTTAAATTAAGACATGATTAACTCTTTTTTTAGAGATTTGATGTTCAAATCCCCTCCCTCTACACTAGTACAATTTCACGATTTTATGACATTGAAAAATTGTCATAAACACATGATTAACTCTTTTTTTTTTTTAGTCGTAGAAGCCACCATCAAGAAACCTCCGACACATCCAAAAATCTGTCATAATTCACTATTTTATGACAATTAATGGTTGTAAAAAAAAATGTATTTAAGACATTTGTTAAAAGTCATGAAAAACTTTTTATGACAATTAAATATTGTCAATAAACACCTCCTAAAACCGACATTTTCGTCAAGATATATATATTAACGACAATTTTTAAACGTCATAGAAATTCTGTTAATGACAGAATAAAAATATTAATGACAGTATTTTTGTGTCAAGTATTTAATTTCTATGATAGTTTGTAGTTGCCAACAAAAAAAATTATTCAAAACTCAGTTTACTTAAGCTAAATAAGCACAGTAAGAAGCCCATGTTAAGTTGATGCTAAAATGAAGGAGAGAGACACATTTTATAGCAAAAGATTGGTTGTCCAAAACACAAATTTTTCGATGTGGGATAAATGAAGTTTTTATGACATTGAAAAACCGTAATAAATTTGTGAGTTGAAAAACTGTCATAAATTAACACAAAAATCTCCAATTTTTCCCACAAAAAATGCGCCTCCAGTCATCTATGATAGTTTTTTTTATCTAAGTCATGAATTTATGATATTGAAAAATTGTCATGAATTTATGATGTTGAAAAACTGTCATAAATTTTCGAAAATACGAAAATCTATGTTTTTTTGTTGCAAAAAACGCGTCTCCATCATTTTATGATAGTTTTTTTTTCTCCCTCCCTTTTATTTGGCTTAGCTATGATGTCATAGAATGTCAAAATTGTAGTAGTGAAAAAGTATAAAGGGATGTTACGTATGAATAAAAGATTAACTTGATTGTAATTTATCAACTTAAACTTGATGGGATGAATTTTTGATATGCAGGTGAAAGAGAAGTCCTCAAATGAGAGAAAATGGAAGACAACAAAAGTCAAGCTAGAAGAGCACATTTACATACCCAAATTTCCTATGGAACTGTCACCATATGAATACGATGCTTACTTTGATGAATATGCAACAAAAATAGGCACAAAACCCTTTTCACAAAGCAGTCCTTTGTGGGAAATTCACATTTTCAATTACCCTACAAGCCATGCAAAAAGTAATGTAATTTTGAAAATTCATCATTCTATAGCCGATGGCTATTGTCTTATGAACACTCTTTTAAGTTGCCTTAAGAGAGCCGATGATCCTTCTCTTCCTCTAACTTTTCCATCTCGAAAACGCTCTCAACAACCCAAAAATATTCCCTTTTTTGGTCATTTTAGCCATTTGCCTTCTCTTTTCTCTTCTATTTCCAACTTTGTTTGGAGTATCATGAAGAACACTTTTGTTGAAGATGATCCAACACCCATCAAACCAGAAGGAGATTCCATGCAGCTCGTTAAGCCAATTGCCATATCCACCATCACCTTTTCTCTTGATCAAATCAAACAAATCAAAAATAAGCTTAACGCGGTATGGTATTTATACATTTTTCATATTTGATCAAAGATCATTTAAAAATATCCTATCTATATATTAATTATTCATAGATTTGAGAGTTTGATGTCACAATCAACATTATATTTGTGATATATATATTTACCATCTAGAAAAAAAAAACATATATATTGATATAACTTTTTATTCCGTTGTTGTGATAAAATACTTATGATAGATCGTTTAAACTTCAAAAATATGTCATATCTAATAGGTGATTCTTATGTTAACTTTATTGCTTTGATATTTATGCATAATGATACATGATACTATTTAGAAATTGCATAACAATTTTATTTTTAAAATAGTTTAAGCATTTCATATGGTAAGCGTTAAACTAATTAATAGAGTCAACAATAATAACTAAAGGGACATAAGATTAAATCTTCTCTAACTTATTAGAAATTGAACTTTAAAATACGAAGCACATGGACCAATTAAAAGTTGTGAGGCGATCATTTTAATTTAACCCAAACAAGTAGACCCTTGATTTTTTCTTTTTTTTTTTTGAGTTCGACATCATTTAAGGGTCGAGTTATATTGGGGGAATTCAAACCACAGACTTTTTGATCGAGGGTATATATTTTGATTGAGAAGTGTAAACTATTTCCTAATTATATTTCAATAATTTATTTTATTTTCATTTAATCCTTCTAGTTATATCCCTGGATATTTTATTAATATATCTATGAATATTTTTATTATAACTTCTATATTTCCATCGACATCGATATTCTAATTAAAAACTCTTTTGGGCGATTTTGAATTTATTGATAGAGCGTTAATGACGTGCTCACGGGAATCATATTCTTGGGAATTCGACTATACATGGAAGAACATAGCCCTCAATCAAGCGAGGCAAATTCAAGTGCATTGATTTTACTCAATACTAGAAAGGCTAAGGCTTACAAATCAGTTAAAGAGATGGTTAAAAGCGATACTGATGCACCATGGGGAAATAAAATCGCTTTTTTGCCAGTCCCAATACCCAAACTAATTGATTCTCCTCTTTCAACTTGCCCTTTGAAATTTATAAAGGAAGCCAAACAAAAAATCATGTTGCAAAGAAGCCCTCTCTCTGTTTTCATGGCTGCTAAAGTTTTTGATATGGTGAAGCATGTGACTGCAGACCCTGAGGTGAAAATTTAACCCCCCCTTTTTTTACCTTTTCTTATAATTGGTTTAAAAGAGAAAGGTAAATTGGATAACCCATTTTTACCTTTTTCTTGTGTGTTAGGTTGGAGCAAAGTTGTTCAAAAGGAAGCTTAAGAATTCAAGCATTATGATATCAAATATGATTGGACCTGTGGAGAAAATGGCTTTGGCCAATCATCCAGTTAAAGGTTTATACTTTACAGTCCCTGGCATGCCTCAGGTATCTAATCTCAACTATTCTCTTGAAAACTAAAAGAATTGAGATCTCTCTTTGAATTTAATTTGAATGGTCTTTAGGATTTTGGATGTGGATCGCGTATTTGTCCTATAGATTTGTCAAGTTTAAAGTTTGTGTTAAAGACGACAGACTCTCCATATATTTTCATTGAAATTGTATGATATTGTCTACCTTGTCAGCCATGTTAGCTTTGTTTTTAGTTTCAATCACAACGTCTCGTATAACACAGATATAGTTGTCATCACTTATACTCATAATCTTCTCACTTGAATCTCAAGCTCAAGATTCCAAACTTGAAAACTTCTCTCCCCACCCCCAACTAAAGCGTAAGGTCTTTATTCTACCAATCAAAAGTTTTAAGACAATAGTAATTTTTATATATTTACACACACATATATATATATTTAATATTAACATGTGTCTCTCTTGAATTGTAGAGTCTAATGATAACAATTGTGAGTTACATGGGAGACTTGAGGATTGTGTTTGGAGGTGAGAAGTGCTTCATAGATCATCAGAAGTTGAAAGCATGCATTGAAGATGCCTACCAAAGGACACTGATCAAAGCTGTTAAATAAAAGTAATTTTTAATATTTTTCGAGGTCTGTGAAAGCACGTAAAAAGTTAATGCAATTTCAGTTTTTTAGGTTTTGCGTTTAATTTATGCTTGTGAGTGGCAACCAAAGTTCATGTGTGAGAC

mRNA sequence

CTTTATTGTTTCAATTTCCAACAGAATCTCCCTCATTTAGATCAAATAATTTGGGATCATCGGCAGTGAAACATATATATATATATATTAGTAATGGAGATTTTGGAAGATTCAACAGCAACAACTCCCATGAGCCCTTTGAGCCAATACTTCAACACCTCTGAAATGTGTGTTTCTGTTTTGGGTGTGATTGAACTCGAATCTCCAATTACTCGCTACGAAGCCATGTCAATTATCACTGATGTTCTCATTCCTGCCAATCCACGTTTCACTTCCATCATGGTGAAAGAGAAGTCCTCAAATGAGAGAAAATGGAAGACAACAAAAGTCAAGCTAGAAGAGCACATTTACATACCCAAATTTCCTATGGAACTGTCACCATATGAATACGATGCTTACTTTGATGAATATGCAACAAAAATAGGCACAAAACCCTTTTCACAAAGCAGTCCTTTGTGGGAAATTCACATTTTCAATTACCCTACAAGCCATGCAAAAAGTAATGTAATTTTGAAAATTCATCATTCTATAGCCGATGGCTATTGTCTTATGAACACTCTTTTAAGTTGCCTTAAGAGAGCCGATGATCCTTCTCTTCCTCTAACTTTTCCATCTCGAAAACGCTCTCAACAACCCAAAAATATTCCCTTTTTTGGTCATTTTAGCCATTTGCCTTCTCTTTTCTCTTCTATTTCCAACTTTGTTTGGAGTATCATGAAGAACACTTTTGTTGAAGATGATCCAACACCCATCAAACCAGAAGGAGATTCCATGCAGCTCGTTAAGCCAATTGCCATATCCACCATCACCTTTTCTCTTGATCAAATCAAACAAATCAAAAATAAGCTTAACGCGAGCGTTAATGACGTGCTCACGGGAATCATATTCTTGGGAATTCGACTATACATGGAAGAACATAGCCCTCAATCAAGCGAGGCAAATTCAAGTGCATTGATTTTACTCAATACTAGAAAGGCTAAGGCTTACAAATCAGTTAAAGAGATGGTTAAAAGCGATACTGATGCACCATGGGGAAATAAAATCGCTTTTTTGCCAGTCCCAATACCCAAACTAATTGATTCTCCTCTTTCAACTTGCCCTTTGAAATTTATAAAGGAAGCCAAACAAAAAATCATGTTGCAAAGAAGCCCTCTCTCTGTTTTCATGGCTGCTAAAGTTTTTGATATGGTGAAGCATGTGACTGCAGACCCTGAGGTTGGAGCAAAGTTGTTCAAAAGGAAGCTTAAGAATTCAAGCATTATGATATCAAATATGATTGGACCTGTGGAGAAAATGGCTTTGGCCAATCATCCAGTTAAAGGTTTATACTTTACAGTCCCTGGCATGCCTCAGAGTCTAATGATAACAATTGTGAGTTACATGGGAGACTTGAGGATTGTGTTTGGAGGTGAGAAGTGCTTCATAGATCATCAGAAGTTGAAAGCATGCATTGAAGATGCCTACCAAAGGACACTGATCAAAGCTGTTAAATAAAAGTAATTTTTAATATTTTTCGAGGTCTGTGAAAGCACGTAAAAAGTTAATGCAATTTCAGTTTTTTAGGTTTTGCGTTTAATTTATGCTTGTGAGTGGCAACCAAAGTTCATGTGTGAGAC

Coding sequence (CDS)

ATGGAGATTTTGGAAGATTCAACAGCAACAACTCCCATGAGCCCTTTGAGCCAATACTTCAACACCTCTGAAATGTGTGTTTCTGTTTTGGGTGTGATTGAACTCGAATCTCCAATTACTCGCTACGAAGCCATGTCAATTATCACTGATGTTCTCATTCCTGCCAATCCACGTTTCACTTCCATCATGGTGAAAGAGAAGTCCTCAAATGAGAGAAAATGGAAGACAACAAAAGTCAAGCTAGAAGAGCACATTTACATACCCAAATTTCCTATGGAACTGTCACCATATGAATACGATGCTTACTTTGATGAATATGCAACAAAAATAGGCACAAAACCCTTTTCACAAAGCAGTCCTTTGTGGGAAATTCACATTTTCAATTACCCTACAAGCCATGCAAAAAGTAATGTAATTTTGAAAATTCATCATTCTATAGCCGATGGCTATTGTCTTATGAACACTCTTTTAAGTTGCCTTAAGAGAGCCGATGATCCTTCTCTTCCTCTAACTTTTCCATCTCGAAAACGCTCTCAACAACCCAAAAATATTCCCTTTTTTGGTCATTTTAGCCATTTGCCTTCTCTTTTCTCTTCTATTTCCAACTTTGTTTGGAGTATCATGAAGAACACTTTTGTTGAAGATGATCCAACACCCATCAAACCAGAAGGAGATTCCATGCAGCTCGTTAAGCCAATTGCCATATCCACCATCACCTTTTCTCTTGATCAAATCAAACAAATCAAAAATAAGCTTAACGCGAGCGTTAATGACGTGCTCACGGGAATCATATTCTTGGGAATTCGACTATACATGGAAGAACATAGCCCTCAATCAAGCGAGGCAAATTCAAGTGCATTGATTTTACTCAATACTAGAAAGGCTAAGGCTTACAAATCAGTTAAAGAGATGGTTAAAAGCGATACTGATGCACCATGGGGAAATAAAATCGCTTTTTTGCCAGTCCCAATACCCAAACTAATTGATTCTCCTCTTTCAACTTGCCCTTTGAAATTTATAAAGGAAGCCAAACAAAAAATCATGTTGCAAAGAAGCCCTCTCTCTGTTTTCATGGCTGCTAAAGTTTTTGATATGGTGAAGCATGTGACTGCAGACCCTGAGGTTGGAGCAAAGTTGTTCAAAAGGAAGCTTAAGAATTCAAGCATTATGATATCAAATATGATTGGACCTGTGGAGAAAATGGCTTTGGCCAATCATCCAGTTAAAGGTTTATACTTTACAGTCCCTGGCATGCCTCAGAGTCTAATGATAACAATTGTGAGTTACATGGGAGACTTGAGGATTGTGTTTGGAGGTGAGAAGTGCTTCATAGATCATCAGAAGTTGAAAGCATGCATTGAAGATGCCTACCAAAGGACACTGATCAAAGCTGTTAAATAA

Protein sequence

MEILEDSTATTPMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSIITDVLIPANPRFTSIMVKEKSSNERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSSPLWEIHIFNYPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQPKNIPFFGHFSHLPSLFSSISNFVWSIMKNTFVEDDPTPIKPEGDSMQLVKPIAISTITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEHSPQSSEANSSALILLNTRKAKAYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSPLSTCPLKFIKEAKQKIMLQRSPLSVFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALANHPVKGLYFTVPGMPQSLMITIVSYMGDLRIVFGGEKCFIDHQKLKACIEDAYQRTLIKAVK
Homology
BLAST of Tan0013338 vs. ExPASy Swiss-Prot
Match: Q9M3B2 (Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=WSD5 PE=2 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.7e-42
Identity = 131/452 (28.98%), Postives = 223/452 (49.34%), Query Frame = 0

Query: 12  PMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSIITDVLIPANPRFTSIMVKEKSSN- 71
           P+SP ++ F+  E    ++ V+ L++ I     +  I   L+  +PRF+S +V   ++N 
Sbjct: 27  PLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLM-RHPRFSSKLVNNCNNNR 86

Query: 72  -ERKWKTTKVKLEEHIYIPKFPME-LSPYEYDAYFDEYATKIGTKPFSQSSPLWEIHIFN 131
            E+KW  T V +E+H+ IPK   + +     D + + Y + + T P   S PLWE+H+ +
Sbjct: 87  QEQKWVRTNVVVEDHVIIPKIQTQHIENANADVFLESYVSDLTTIPLDTSKPLWEVHLLD 146

Query: 132 YPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADD----PSLPL-TFPSRKRSQQPKN 191
             TS A++  +L+IHHS+ DG  +M+ +L+C ++  +    PSLP    PS   S    +
Sbjct: 147 LKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNRPSSGSSSLKTS 206

Query: 192 IPFFGHFSHLPSLFSSISNFVWS--------IMKNTFVEDDPTPIKPEGD-SMQLVKPIA 251
              +  F  L  +  S +  V +        I    F++D  TPIK  GD  +   K + 
Sbjct: 207 SRCYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPIK--GDFKLSKGKRMC 266

Query: 252 ISTITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEH--SPQSSEANSS------- 311
           +   T SLD IK IKN +  +VNDV+ G+   G+  Y++      + S+ NSS       
Sbjct: 267 MVHRTVSLDDIKLIKNAMKMTVNDVVLGVSQAGLSQYLKRRYGEQEESKRNSSNIPKGIR 326

Query: 312 --ALILLNTRKAKAYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSPLSTCPLKFIKEA 371
             A +L+N R     + + +M+   +   WGN I ++  P        L   PLK ++ A
Sbjct: 327 LRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYIIFP----FSIALCDDPLKHLRRA 386

Query: 372 KQKIMLQRSPLSVFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMAL 431
           K  I  +++ L   +   V  ++ + T   +  A +  R L N+++  SN++GPVE+++ 
Sbjct: 387 KSTIDRKKNSLEAVLTFVVGKILLN-TLGVQRAANVLNRALSNTTMSFSNLVGPVEEISF 446

Query: 432 ANHPVKGLYFTVPGMPQSLMITIVSYMGDLRI 436
             H V  +  +V G P +L +   SYM  L I
Sbjct: 447 YGHTVTYIAPSVYGHPHALTMHFQSYMNKLTI 470

BLAST of Tan0013338 vs. ExPASy Swiss-Prot
Match: Q5KS41 (Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=3702 GN=WSD11 PE=1 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 1.1e-41
Identity = 121/438 (27.63%), Postives = 223/438 (50.91%), Query Frame = 0

Query: 12  PMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSII--TDVLIPANPRFTSIMVKEKSS 71
           P+SP S+ FN+ +   ++  ++ + S + + +  +II   +  +  +PRF+SI+ +  + 
Sbjct: 16  PLSPCSRLFNSPDFNCAI--IVTMGSKV-KGDTPAIIHGLEHTLVNHPRFSSIL-EMNNG 75

Query: 72  NERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSSPLWEIHIFNY 131
            + +W  TKVK+EEH+ +P    ++     D Y ++Y +K+ T P   S PLWE+H+   
Sbjct: 76  KKPRWVRTKVKVEEHVIVPDVDPDIE--NPDQYLEDYISKLTTIPMDLSKPLWEMHLLGV 135

Query: 132 PTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDP-SLPLTFPSRKRSQQPKNIPFF- 191
            TS+A+S  ILKIHHS+ DG  LM+ LL+C ++  DP +LP     +KR     N  FF 
Sbjct: 136 KTSNAESYAILKIHHSLGDGMSLMSLLLACTRKTSDPEALPTVAVHKKRFGPSCNSGFFN 195

Query: 192 -------GHFSHLPSLFSSISNFVWSIMKNTFVEDDPTPIKPEGDSMQLVKPIAISTITF 251
                  G +  L  LF++  + +   +    + D  TP+  +  S  + K      I+F
Sbjct: 196 KIWWLFVGLWFILRLLFNTFVDILMFALTIFVLRDTETPLLAKPGSELIPKRFVHRIISF 255

Query: 252 SLDQIKQIKNKLNASVNDVLTGIIFLGI-----RLYMEEHSPQSSEA----NSSALILLN 311
             D +K +KN +  +VNDVL G+   G+     R Y +E +P+S E+       + I++N
Sbjct: 256 --DDVKLVKNAMKMTVNDVLLGVTQAGLSRYLSRKYDQEATPKSKESMRRIRLRSAIMIN 315

Query: 312 TRKAKAYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSPLSTCPLKFIKEAKQKIMLQR 371
            R     +++ +M+   +   WGN   ++ +P        L T PL+++++AK  I  ++
Sbjct: 316 LRPNAGIEALADMMAKKSKCRWGNLFGYILLP----FSVGLETDPLEYVRQAKATIDRKK 375

Query: 372 SPLSVFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALANHPVKGL 430
             L    +   F ++  V    +    L ++ + ++++  SN++GP E++    HP+  +
Sbjct: 376 HSLEAVFSMAFFKLILKVLG-LKASVVLVRKVIHSTTLSFSNVVGPKEEITFHGHPLNYI 435

BLAST of Tan0013338 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 2.7e-40
Identity = 115/450 (25.56%), Postives = 217/450 (48.22%), Query Frame = 0

Query: 12  PMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSIITDVLIPANPRFTSIMVKEKSSNE 71
           P+SP ++ F+  E   +++ VI  +S +     +    +  I  +PRF+S +V +++   
Sbjct: 19  PLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKESFI-RHPRFSSKLVTDENGQN 78

Query: 72  RKWKTTKVKLEEHIYIPKF-PMELSPYEYDAYFDEYATKIGTKPFSQSSPLWEIHIFNYP 131
           ++W  T V +E+H  +PK  P  +     +A+ ++Y + +   P   S PLWE+H+ +  
Sbjct: 79  QRWVRTNVVVEDHFIVPKIKPQNIE--NSNAFLEDYVSDLMKIPLDTSRPLWELHLLDLK 138

Query: 132 TSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQPKNIPFFGHF 191
           TS A++  +LKIHHS+ DG  +M+ +L+C+++  +P    + P + RS    ++   G  
Sbjct: 139 TSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLLTTGSR 198

Query: 192 SHLPSLF-------------SSISNFVWSIMKNTFVEDDPTPIKPEGDSMQL-VKPIAIS 251
           S    L+             +++ + +  I+   FV+D  TPIK  GD +    K + + 
Sbjct: 199 SDSRLLWLVKVIWTAVILGLNTVCDALEFIVTTLFVKDTETPIK--GDFLSTKSKQLRLV 258

Query: 252 TITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEHSPQSSEANSS----------- 311
             T SLD IK  KN +N ++NDV+ G+   G+  Y+     +    N             
Sbjct: 259 HRTVSLDDIKLTKNAMNMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKLPKRIRLR 318

Query: 312 ALILLNTRKAKAYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSPLSTCPLKFIKEAKQ 371
           + +L+N R     + + +M++  +   WGN   ++  P        L   PL+ ++ A++
Sbjct: 319 SALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGYVVFP----FSIALRDDPLEHLEIAQK 378

Query: 372 KIMLQRSPLSVFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALAN 431
            I  +++     M   +F  +       +V A +  R + N+++  SNM+GPVE+++   
Sbjct: 379 TISRKKNSYGA-MLTYIFCRIIVKLLGIKVAASIINRMVSNTTMTFSNMVGPVEEVSFYG 438

Query: 432 HPVKGLYFTVPGMPQSLMITIVSYMGDLRI 436
           HP+     +  G P +L I   SYM  + I
Sbjct: 439 HPITYFASSAYGHPHALTIHCQSYMNKMTI 458

BLAST of Tan0013338 vs. ExPASy Swiss-Prot
Match: Q9M3B1 (Wax ester synthase/diacylglycerol acyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=WSD6 PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 1.3e-39
Identity = 134/510 (26.27%), Postives = 233/510 (45.69%), Query Frame = 0

Query: 5   EDSTATTPMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSIITDVLIPANPRFTSIMV 64
           E+     P+SP ++ F+  E    V+ VI ++  I     +  +   LI  +PRF+S MV
Sbjct: 20  EEVEEEQPLSPAARVFHAPEFNCYVISVIGIKKKIDPDVIIEGLKQTLI-RHPRFSSKMV 79

Query: 65  KEKSSNERK----WKTTKVKLEEHIYIPKFPME-LSPYEYDAYFDEYATKIGTKPFSQSS 124
                N+++    W  T V + +H+ +     + +     DA+ + Y + + T P   S 
Sbjct: 80  STSVGNKKRQTQSWVRTNVVVTDHVIVSDIQTQNIENGNADAFLETYVSNLTTVPLDISK 139

Query: 125 PLWEIHIFNYPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQ 184
           PLW++H+ +  TS A++  +LK HHS+ DG  LM  +L+C+++  +P    + P++ RS 
Sbjct: 140 PLWQLHLLDLKTSDAENVAVLKFHHSLGDGMSLMALVLACMRKTSNPDELPSLPNQNRSS 199

Query: 185 QPKNIPFFGHFSHLPSLFSSISNFVWS---------------IMKNTFVEDDPTPIKPEG 244
              +    G  S   S F  +   +WS               I    F++D  TPIK  G
Sbjct: 200 SRSSRLMAG--SRGDSRFLWLVMVIWSAIMLVLNTVCDALEFIATTMFLKDTETPIK--G 259

Query: 245 D-SMQLVKPIAISTITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEH-------- 304
           D      K + +   T SLD IK IKN +  +VNDV+ G+   G+  Y++          
Sbjct: 260 DFRFSKSKRMCLVHRTVSLDDIKLIKNTMKMTVNDVVLGVSQAGLSQYLDRRYGEKKKKV 319

Query: 305 -SPQSSEANSS---------ALILLNTRKAKAYKSVKEMVKSDTDAPWGNKIAFLPVPIP 364
              Q S+  ++         + +L+N R     + + +M+   +   WGN I ++  P  
Sbjct: 320 GEDQDSKRKATDMPKRIRLRSALLVNLRPNTGIQDLADMMAKGSTCRWGNWIGYIVFP-- 379

Query: 365 KLIDSPLSTCPLKFIKEAKQKIMLQRSPLSVFMAAKVFDMVKHV--TADPEVGAKLFKRK 424
                 L   PL+ ++ AK+ I  +++ L    AA  F   K +  T   +V AK+  R 
Sbjct: 380 --FSIGLRDDPLQHLRRAKRIIDRKKNSLE---AALTFVAGKFILKTFGVQVAAKIINRA 439

Query: 425 LKNSSIMISNMIGPVEKMALANHPVKGLYFTVPGMPQSLMITIVSYMGDLRIVFGGEKCF 462
           L N+++  SN+IGP+E+++   HP+  +  +V G P +L +   SYM  + I    +   
Sbjct: 440 LSNTTMSFSNLIGPIEEISFYGHPITYMAPSVYGHPHALTMHFQSYMNQMTISLTVDPTV 499

BLAST of Tan0013338 vs. ExPASy Swiss-Prot
Match: Q94CK0 (Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=WSD7 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.1e-38
Identity = 123/448 (27.46%), Postives = 218/448 (48.66%), Query Frame = 0

Query: 12  PMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSIITDVL---IPANPRFTSIMVKEKS 71
           P+SP+++ F +  + +  + ++  ++ I       ++ D L   +  +PRF+SI+    S
Sbjct: 8   PVSPMARVFQSPGIDLCAVTIMGFKTKIN----PDVVLDALKQNVSKHPRFSSIL----S 67

Query: 72  SNERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSSPLWEIHIFN 131
            N  KW  T+V +E+H+ +P    E       ++ D+Y +++   P  +S PLW+IHI N
Sbjct: 68  DNGAKWIETEVNVEDHVIVPYIDAEEIGEGGQSFIDDYMSRLTMIPLDRSRPLWDIHILN 127

Query: 132 YPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSL-PLTFPS-RKRSQQPKNIPF 191
             TS A++   ++ HHS+ DG  L++ +L+C  +  DP +     PS ++R+    ++  
Sbjct: 128 VKTSEAEAVGFIRSHHSLGDGMSLISLMLACTHKTSDPDMFSNAIPSMKRRATMSHSLKT 187

Query: 192 FGHFSHLPSLFSSISNFVWS--------IMKNTFVEDDPTPIKPEGDSMQLVKPIAISTI 251
            G F        S    +W+        +    F++D  TP+K   D      P      
Sbjct: 188 KGWFLRSIFTIGSTMRLLWNTTIDMLLLLATVLFLKDTKTPLKAGADVRS--NPKRFYHR 247

Query: 252 TFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYM--------EEHSPQSSEANS------ 311
             SLD IK IKN +N ++NDVL GI    +  Y+        EE    +S  N+      
Sbjct: 248 IISLDDIKLIKNAMNMTINDVLFGITQASLSHYLNRQYGTKKEEDGALTSYRNNLPDGIR 307

Query: 312 -SALILLNTRKAKAYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSPLSTCPLKFIKEA 371
                 +N R    +K + +M+  D+   WGN  +F+ +P        L T PL ++K +
Sbjct: 308 FRVACTVNLRSDIGFKPLADMMVKDSKCRWGNYFSFIFLP----FTIGLQTDPLVYLKMS 367

Query: 372 KQKIMLQRSPLSVFMAAKVFDMVKHV--TADPEVGAKLFKRKLKNSSIMISNMIGPVEKM 430
           K   M+ R   S + AA V+ ++K V      +  A+LF R ++N++  +SN+IGP+E++
Sbjct: 368 KS--MMARKKHS-YHAALVYFIIKIVLKVFGAKAAAELFDRPVRNTTTCVSNVIGPMEEI 427

BLAST of Tan0013338 vs. NCBI nr
Match: XP_022147882.1 (O-acyltransferase WSD1-like [Momordica charantia] >XP_022147883.1 O-acyltransferase WSD1-like [Momordica charantia])

HSP 1 Score: 766.9 bits (1979), Expect = 9.9e-218
Identity = 384/467 (82.23%), Postives = 424/467 (90.79%), Query Frame = 0

Query: 1   MEILEDSTATTPMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSIITDVLIPANPRFT 60
           MEILEDS  TTPMSPLSQYFNTSEMCVSVLGV+ELESPI+  +A+SI+TDVL+PANPRFT
Sbjct: 1   MEILEDS--TTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFT 60

Query: 61  SIMVKEKSSNERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSSP 120
           SIMVKEKS+ ERKWKT +V   +HIY+P FP +LSPYEYDAYFDEYATK  TKPFSQS P
Sbjct: 61  SIMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIP 120

Query: 121 LWEIHIFNYPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQ 180
           LWEIH+FNYPTSHA  ++I K+HHSIADG+CLMNTLLSCLKRADDPSLPLTFPSRK S+Q
Sbjct: 121 LWEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQ 180

Query: 181 PKNIPFFGHFSHLPSLFSS-ISNFVWSIMKNTFVEDDPTPIKPEGDSMQLVKPIAISTIT 240
           PKN   FG  SHLP   SS +SNF WS+MKNTFVEDDPTPIKPEGD MQLVKPIAIST+T
Sbjct: 181 PKNDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMT 240

Query: 241 FSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEHSPQSSEANSSALILLNTRKAKAYK 300
           FSLDQIKQIKN LN S+NDVLTGIIF+G+RLYM+EH+PQSSE NSSALILLNTRKAKAYK
Sbjct: 241 FSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYK 300

Query: 301 SVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSPLSTCPLKFIKEAKQKIMLQRSPLSVFMA 360
           SVKEM+++DTDAPWGN+IAFLPVPIPKLIDS LS  PL+F+KEAK+KIMLQR+ LSVFMA
Sbjct: 301 SVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMA 360

Query: 361 AKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALANHPVKGLYFTVPGMP 420
           AKVFD+V HVT  PEVGAKLFKRKLKNSSIMIS+MIGP+EKMALAN PVKGLYFTVPGMP
Sbjct: 361 AKVFDIVNHVTG-PEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMP 420

Query: 421 QSLMITIVSYMGDLRIVFGGEKCFIDHQKLKACIEDAYQRTLIKAVK 467
           QSL+ITIVSYMGDLRIVFGGEKCFID QKLK CIEDA++RTL+KA K
Sbjct: 421 QSLLITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMKAAK 464

BLAST of Tan0013338 vs. NCBI nr
Match: XP_011659709.2 (O-acyltransferase WSD1 [Cucumis sativus] >KGN43701.2 hypothetical protein Csa_017048 [Cucumis sativus])

HSP 1 Score: 748.0 bits (1930), Expect = 4.7e-212
Identity = 385/472 (81.57%), Postives = 426/472 (90.25%), Query Frame = 0

Query: 1   MEILEDSTATTPMSPLSQYFNTSEMCVSVLGVIELESPITRYEAM-SIITDVLIPANPRF 60
           MEI EDSTA  PMSPLSQYFNT EMCVSVLGVIELE+PI+ +E + SI+TDVL+PANPRF
Sbjct: 1   MEIYEDSTA--PMSPLSQYFNTCEMCVSVLGVIELETPISSWEDISSIVTDVLVPANPRF 60

Query: 61  TSIMVKEKSSNERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSS 120
           TSIMVKEK + ERKWKTTKV  ++HIYIP FPMELS YEYDAYFDEYATK  TKPFSQ+ 
Sbjct: 61  TSIMVKEK-TEERKWKTTKVNPKDHIYIPNFPMELSSYEYDAYFDEYATKTATKPFSQAI 120

Query: 121 PLWEIHIFNYPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQ 180
           PLWEIH+FNYPT+HA  ++I K+HHSIADG+CLMNTLLSCLKRADDPSLPLTFPSR+RS+
Sbjct: 121 PLWEIHVFNYPTTHATCSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSK 180

Query: 181 QPKNIPFFGHFSHLPS-LFSSISNFV----WSIMKNTFVEDDPTPIKPEGDSMQLVKPIA 240
           QP N P F   SH P+  FSSISNFV    WSIMKNTFVEDDPTPIKP+ DSMQLVKPIA
Sbjct: 181 QPGNEPKFFRLSHFPARFFSSISNFVLNFGWSIMKNTFVEDDPTPIKPQKDSMQLVKPIA 240

Query: 241 ISTITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEHSPQSSEANSSALILLNTRK 300
           IST+TFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYM+EH+P+SS ANSSALILLNTRK
Sbjct: 241 ISTMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHNPESSGANSSALILLNTRK 300

Query: 301 AKAYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSP-LSTCPLKFIKEAKQKIMLQRSP 360
           AKAYKSVKEMVK D+DAPWGNKIAFLP+PIPKLIDSP +S+ PL+F+++ K+KIMLQRSP
Sbjct: 301 AKAYKSVKEMVKKDSDAPWGNKIAFLPIPIPKLIDSPVVSSTPLEFVEKVKEKIMLQRSP 360

Query: 361 LSVFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALANHPVKGLYF 420
           LSVF+AAKVF+++K+VT  PE+GAKLFKRKLKNSSIMISNMIGPVEKMAL N PVKGLYF
Sbjct: 361 LSVFLAAKVFEILKNVTG-PEIGAKLFKRKLKNSSIMISNMIGPVEKMALVNLPVKGLYF 420

Query: 421 TVPGMPQSLMITIVSYMGDLRIVFGGEKCFIDHQKLKACIEDAYQRTLIKAV 466
           TVPGMPQSLMITIVSYMGDLRIVFGGEKCFI+ QKLK CIEDA+QR L KAV
Sbjct: 421 TVPGMPQSLMITIVSYMGDLRIVFGGEKCFINQQKLKVCIEDAFQRILAKAV 468

BLAST of Tan0013338 vs. NCBI nr
Match: XP_022952997.1 (O-acyltransferase WSD1-like [Cucurbita moschata])

HSP 1 Score: 720.3 bits (1858), Expect = 1.1e-203
Identity = 371/468 (79.27%), Postives = 407/468 (86.97%), Query Frame = 0

Query: 1   MEILEDSTATTPMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSIITDVLIPANPRFT 60
           MEILEDS A  PMSPLSQYFNTS MCVSVLG IELE+PI+   AM+I T VLIPANPRF+
Sbjct: 1   MEILEDSAA--PMSPLSQYFNTSAMCVSVLGFIELEAPISASHAMNIGTHVLIPANPRFS 60

Query: 61  SIMVKEKSSNERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSSP 120
           SIMVKEK   ERKWK  +V LE+HIY PKFP++LSPYEYDAYFDEYAT   TKPFSQS P
Sbjct: 61  SIMVKEKWFGERKWKMVEVNLEDHIYTPKFPVDLSPYEYDAYFDEYATNTATKPFSQSIP 120

Query: 121 LWEIHIFNYPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQ 180
           LWEIH+FNYPT+HA   +I K+HHSIADG+CLMNTLLS LKRADDPSLPLTFPSRKRS+Q
Sbjct: 121 LWEIHVFNYPTTHAPCTLIFKVHHSIADGFCLMNTLLSFLKRADDPSLPLTFPSRKRSKQ 180

Query: 181 PKNIPFFGHFSHLP-----SLFSSISNFVWSIMKNTFVEDDPTPIKPEGDSMQLVKPIAI 240
           P+N   FG  SH P     SL + +SNF +SI  NTFVEDDPTPIK EGDSMQLVKPIAI
Sbjct: 181 PENAHDFGRLSHFPACLFLSLRNFLSNFGYSI-ANTFVEDDPTPIKHEGDSMQLVKPIAI 240

Query: 241 STITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEHSPQSSEANSSALILLNTRKA 300
           ST+TFSLDQIKQIK KLNA VNDV+TGIIFLGIRLYM+EHSP S EANSSALILLNTRKA
Sbjct: 241 STMTFSLDQIKQIKTKLNARVNDVITGIIFLGIRLYMQEHSPDSWEANSSALILLNTRKA 300

Query: 301 KAYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSPLSTCPLKFIKEAKQKIMLQRSPLS 360
           KAYKSV+EMVK+D+D PWGN+IAFLPVPIPKL+DS LST PL+F+KEAK+ IMLQRSPLS
Sbjct: 301 KAYKSVQEMVKTDSDVPWGNRIAFLPVPIPKLMDSTLSTAPLEFVKEAKKNIMLQRSPLS 360

Query: 361 VFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALANHPVKGLYFTV 420
           VFMAAKVFDM+KHVT  PE+GAKLFKRKL NSS +ISNMIGP EKMAL N PVKGLYFTV
Sbjct: 361 VFMAAKVFDMLKHVTG-PEMGAKLFKRKLNNSSTIISNMIGPTEKMALVNRPVKGLYFTV 420

Query: 421 PGMPQSLMITIVSYMGDLRIVFGGEKCFIDHQKLKACIEDAYQRTLIK 464
           PG+PQSL+ITIVSYMGDLRIVFGGEKCFID  KLKAC+EDA+QR LIK
Sbjct: 421 PGVPQSLIITIVSYMGDLRIVFGGEKCFIDLHKLKACVEDAFQRMLIK 464

BLAST of Tan0013338 vs. NCBI nr
Match: XP_038887587.1 (O-acyltransferase WSD1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 655.6 bits (1690), Expect = 3.2e-184
Identity = 336/428 (78.50%), Postives = 382/428 (89.25%), Query Frame = 0

Query: 1   MEILEDSTATTPMSPLSQYFNTSEMCVSVLGVIELESPITRYEAM-SIITDVLIPANPRF 60
           ME  E+STA  PMSP+SQYFNTSEMCVSVLGVIELE+PI+ +E + SI  DVLIP+NPRF
Sbjct: 1   MENYEESTA--PMSPMSQYFNTSEMCVSVLGVIELETPISAWEEISSIAIDVLIPSNPRF 60

Query: 61  TSIMVKEKSSNERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSS 120
           TS+MVKE S+ ERKWKT K+  ++HIYIP FP ELSPYEYDAYFDEYAT+  TKPFSQS 
Sbjct: 61  TSVMVKESSNGERKWKTIKINPKDHIYIPNFPPELSPYEYDAYFDEYATQTATKPFSQSI 120

Query: 121 PLWEIHIFNYPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQ 180
           PLWEIH+FNYPTSHA  ++I K+HHSIADG+CLMN LLSCLKRADDPSLPLTFPSR+RS+
Sbjct: 121 PLWEIHVFNYPTSHATCSIIFKVHHSIADGFCLMNILLSCLKRADDPSLPLTFPSRQRSK 180

Query: 181 QPKNIPFFGHFSHLPS-LFSSISNFV----WSIMKNTFVEDDPTPIKPEGDSMQLVKPIA 240
           QP+N   F   S+ P  LFSS+SNF+    WSIMKNT VEDDPTPIKPE DSMQLVKPIA
Sbjct: 181 QPENELNFRRRSYFPGHLFSSVSNFLLNFGWSIMKNTIVEDDPTPIKPERDSMQLVKPIA 240

Query: 241 ISTITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEHSPQSSEANSSALILLNTRK 300
           ISTITF LDQIKQIK+KLNASVNDV+TGIIFLGIRLYM+EH+P+SS+ANSSALILLNTRK
Sbjct: 241 ISTITFPLDQIKQIKDKLNASVNDVVTGIIFLGIRLYMQEHNPESSQANSSALILLNTRK 300

Query: 301 AKAYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDS-PLSTCPLKFIKEAKQKIMLQRSP 360
           AKAYKSVKEMVK++TDAPWGNKIAFLPVPIPKLIDS  L++ PL+F+K+AK+KIMLQRSP
Sbjct: 301 AKAYKSVKEMVKTNTDAPWGNKIAFLPVPIPKLIDSTTLASTPLEFVKKAKEKIMLQRSP 360

Query: 361 LSVFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALANHPVKGLYF 420
           +SVF+AAK F+MVK+VT  PEVGAKLFKRKLKNSSIMISNMIGPVEK++L NHPVKG+YF
Sbjct: 361 VSVFLAAKFFEMVKNVTG-PEVGAKLFKRKLKNSSIMISNMIGPVEKISLVNHPVKGIYF 420

Query: 421 TVPGMPQS 422
           TVPGMPQ+
Sbjct: 421 TVPGMPQT 425

BLAST of Tan0013338 vs. NCBI nr
Match: XP_038887586.1 (O-acyltransferase WSD1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 654.8 bits (1688), Expect = 5.5e-184
Identity = 336/427 (78.69%), Postives = 381/427 (89.23%), Query Frame = 0

Query: 1   MEILEDSTATTPMSPLSQYFNTSEMCVSVLGVIELESPITRYEAM-SIITDVLIPANPRF 60
           ME  E+STA  PMSP+SQYFNTSEMCVSVLGVIELE+PI+ +E + SI  DVLIP+NPRF
Sbjct: 1   MENYEESTA--PMSPMSQYFNTSEMCVSVLGVIELETPISAWEEISSIAIDVLIPSNPRF 60

Query: 61  TSIMVKEKSSNERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSS 120
           TS+MVKE S+ ERKWKT K+  ++HIYIP FP ELSPYEYDAYFDEYAT+  TKPFSQS 
Sbjct: 61  TSVMVKESSNGERKWKTIKINPKDHIYIPNFPPELSPYEYDAYFDEYATQTATKPFSQSI 120

Query: 121 PLWEIHIFNYPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQ 180
           PLWEIH+FNYPTSHA  ++I K+HHSIADG+CLMN LLSCLKRADDPSLPLTFPSR+RS+
Sbjct: 121 PLWEIHVFNYPTSHATCSIIFKVHHSIADGFCLMNILLSCLKRADDPSLPLTFPSRQRSK 180

Query: 181 QPKNIPFFGHFSHLPS-LFSSISNFV----WSIMKNTFVEDDPTPIKPEGDSMQLVKPIA 240
           QP+N   F   S+ P  LFSS+SNF+    WSIMKNT VEDDPTPIKPE DSMQLVKPIA
Sbjct: 181 QPENELNFRRRSYFPGHLFSSVSNFLLNFGWSIMKNTIVEDDPTPIKPERDSMQLVKPIA 240

Query: 241 ISTITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEHSPQSSEANSSALILLNTRK 300
           ISTITF LDQIKQIK+KLNASVNDV+TGIIFLGIRLYM+EH+P+SS+ANSSALILLNTRK
Sbjct: 241 ISTITFPLDQIKQIKDKLNASVNDVVTGIIFLGIRLYMQEHNPESSQANSSALILLNTRK 300

Query: 301 AKAYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDS-PLSTCPLKFIKEAKQKIMLQRSP 360
           AKAYKSVKEMVK++TDAPWGNKIAFLPVPIPKLIDS  L++ PL+F+K+AK+KIMLQRSP
Sbjct: 301 AKAYKSVKEMVKTNTDAPWGNKIAFLPVPIPKLIDSTTLASTPLEFVKKAKEKIMLQRSP 360

Query: 361 LSVFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALANHPVKGLYF 420
           +SVF+AAK F+MVK+VT  PEVGAKLFKRKLKNSSIMISNMIGPVEK++L NHPVKG+YF
Sbjct: 361 VSVFLAAKFFEMVKNVTG-PEVGAKLFKRKLKNSSIMISNMIGPVEKISLVNHPVKGIYF 420

BLAST of Tan0013338 vs. ExPASy TrEMBL
Match: A0A6J1D2I7 (Diacylglycerol O-acyltransferase OS=Momordica charantia OX=3673 GN=LOC111016711 PE=4 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 4.8e-218
Identity = 384/467 (82.23%), Postives = 424/467 (90.79%), Query Frame = 0

Query: 1   MEILEDSTATTPMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSIITDVLIPANPRFT 60
           MEILEDS  TTPMSPLSQYFNTSEMCVSVLGV+ELESPI+  +A+SI+TDVL+PANPRFT
Sbjct: 1   MEILEDS--TTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFT 60

Query: 61  SIMVKEKSSNERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSSP 120
           SIMVKEKS+ ERKWKT +V   +HIY+P FP +LSPYEYDAYFDEYATK  TKPFSQS P
Sbjct: 61  SIMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIP 120

Query: 121 LWEIHIFNYPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQ 180
           LWEIH+FNYPTSHA  ++I K+HHSIADG+CLMNTLLSCLKRADDPSLPLTFPSRK S+Q
Sbjct: 121 LWEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQ 180

Query: 181 PKNIPFFGHFSHLPSLFSS-ISNFVWSIMKNTFVEDDPTPIKPEGDSMQLVKPIAISTIT 240
           PKN   FG  SHLP   SS +SNF WS+MKNTFVEDDPTPIKPEGD MQLVKPIAIST+T
Sbjct: 181 PKNDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMT 240

Query: 241 FSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEHSPQSSEANSSALILLNTRKAKAYK 300
           FSLDQIKQIKN LN S+NDVLTGIIF+G+RLYM+EH+PQSSE NSSALILLNTRKAKAYK
Sbjct: 241 FSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYK 300

Query: 301 SVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSPLSTCPLKFIKEAKQKIMLQRSPLSVFMA 360
           SVKEM+++DTDAPWGN+IAFLPVPIPKLIDS LS  PL+F+KEAK+KIMLQR+ LSVFMA
Sbjct: 301 SVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMA 360

Query: 361 AKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALANHPVKGLYFTVPGMP 420
           AKVFD+V HVT  PEVGAKLFKRKLKNSSIMIS+MIGP+EKMALAN PVKGLYFTVPGMP
Sbjct: 361 AKVFDIVNHVTG-PEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMP 420

Query: 421 QSLMITIVSYMGDLRIVFGGEKCFIDHQKLKACIEDAYQRTLIKAVK 467
           QSL+ITIVSYMGDLRIVFGGEKCFID QKLK CIEDA++RTL+KA K
Sbjct: 421 QSLLITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMKAAK 464

BLAST of Tan0013338 vs. ExPASy TrEMBL
Match: A0A6J1GLZ3 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455512 PE=4 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 5.1e-204
Identity = 371/468 (79.27%), Postives = 407/468 (86.97%), Query Frame = 0

Query: 1   MEILEDSTATTPMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSIITDVLIPANPRFT 60
           MEILEDS A  PMSPLSQYFNTS MCVSVLG IELE+PI+   AM+I T VLIPANPRF+
Sbjct: 1   MEILEDSAA--PMSPLSQYFNTSAMCVSVLGFIELEAPISASHAMNIGTHVLIPANPRFS 60

Query: 61  SIMVKEKSSNERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSSP 120
           SIMVKEK   ERKWK  +V LE+HIY PKFP++LSPYEYDAYFDEYAT   TKPFSQS P
Sbjct: 61  SIMVKEKWFGERKWKMVEVNLEDHIYTPKFPVDLSPYEYDAYFDEYATNTATKPFSQSIP 120

Query: 121 LWEIHIFNYPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQ 180
           LWEIH+FNYPT+HA   +I K+HHSIADG+CLMNTLLS LKRADDPSLPLTFPSRKRS+Q
Sbjct: 121 LWEIHVFNYPTTHAPCTLIFKVHHSIADGFCLMNTLLSFLKRADDPSLPLTFPSRKRSKQ 180

Query: 181 PKNIPFFGHFSHLP-----SLFSSISNFVWSIMKNTFVEDDPTPIKPEGDSMQLVKPIAI 240
           P+N   FG  SH P     SL + +SNF +SI  NTFVEDDPTPIK EGDSMQLVKPIAI
Sbjct: 181 PENAHDFGRLSHFPACLFLSLRNFLSNFGYSI-ANTFVEDDPTPIKHEGDSMQLVKPIAI 240

Query: 241 STITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEHSPQSSEANSSALILLNTRKA 300
           ST+TFSLDQIKQIK KLNA VNDV+TGIIFLGIRLYM+EHSP S EANSSALILLNTRKA
Sbjct: 241 STMTFSLDQIKQIKTKLNARVNDVITGIIFLGIRLYMQEHSPDSWEANSSALILLNTRKA 300

Query: 301 KAYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSPLSTCPLKFIKEAKQKIMLQRSPLS 360
           KAYKSV+EMVK+D+D PWGN+IAFLPVPIPKL+DS LST PL+F+KEAK+ IMLQRSPLS
Sbjct: 301 KAYKSVQEMVKTDSDVPWGNRIAFLPVPIPKLMDSTLSTAPLEFVKEAKKNIMLQRSPLS 360

Query: 361 VFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALANHPVKGLYFTV 420
           VFMAAKVFDM+KHVT  PE+GAKLFKRKL NSS +ISNMIGP EKMAL N PVKGLYFTV
Sbjct: 361 VFMAAKVFDMLKHVTG-PEMGAKLFKRKLNNSSTIISNMIGPTEKMALVNRPVKGLYFTV 420

Query: 421 PGMPQSLMITIVSYMGDLRIVFGGEKCFIDHQKLKACIEDAYQRTLIK 464
           PG+PQSL+ITIVSYMGDLRIVFGGEKCFID  KLKAC+EDA+QR LIK
Sbjct: 421 PGVPQSLIITIVSYMGDLRIVFGGEKCFIDLHKLKACVEDAFQRMLIK 464

BLAST of Tan0013338 vs. ExPASy TrEMBL
Match: A0A0A0K2E9 (Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051330 PE=4 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 1.8e-185
Identity = 334/410 (81.46%), Postives = 370/410 (90.24%), Query Frame = 0

Query: 62  IMVKEKSSNERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSSPL 121
           + VKEK + ERKWKTTKV  ++HIYIP FPMELS YEYDAYFDEYATK  TKPFSQ+ PL
Sbjct: 1   MQVKEK-TEERKWKTTKVNPKDHIYIPNFPMELSSYEYDAYFDEYATKTATKPFSQAIPL 60

Query: 122 WEIHIFNYPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQP 181
           WEIH+FNYPT+HA  ++I K+HHSIADG+CLMNTLLSCLKRADDPSLPLTFPSR+RS+QP
Sbjct: 61  WEIHVFNYPTTHATCSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQP 120

Query: 182 KNIPFFGHFSHLPS-LFSSISNFV----WSIMKNTFVEDDPTPIKPEGDSMQLVKPIAIS 241
            N P F   SH P+  FSSISNFV    WSIMKNTFVEDDPTPIKP+ DSMQLVKPIAIS
Sbjct: 121 GNEPKFFRLSHFPARFFSSISNFVLNFGWSIMKNTFVEDDPTPIKPQKDSMQLVKPIAIS 180

Query: 242 TITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEHSPQSSEANSSALILLNTRKAK 301
           T+TFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYM+EH+P+SS ANSSALILLNTRKAK
Sbjct: 181 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHNPESSGANSSALILLNTRKAK 240

Query: 302 AYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSP-LSTCPLKFIKEAKQKIMLQRSPLS 361
           AYKSVKEMVK D+DAPWGNKIAFLP+PIPKLIDSP +S+ PL+F+++ K+KIMLQRSPLS
Sbjct: 241 AYKSVKEMVKKDSDAPWGNKIAFLPIPIPKLIDSPVVSSTPLEFVEKVKEKIMLQRSPLS 300

Query: 362 VFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALANHPVKGLYFTV 421
           VF+AAKVF+++K+VT  PE+GAKLFKRKLKNSSIMISNMIGPVEKMAL N PVKGLYFTV
Sbjct: 301 VFLAAKVFEILKNVTG-PEIGAKLFKRKLKNSSIMISNMIGPVEKMALVNLPVKGLYFTV 360

Query: 422 PGMPQSLMITIVSYMGDLRIVFGGEKCFIDHQKLKACIEDAYQRTLIKAV 466
           PGMPQSLMITIVSYMGDLRIVFGGEKCFI+ QKLK CIEDA+QR L KAV
Sbjct: 361 PGMPQSLMITIVSYMGDLRIVFGGEKCFINQQKLKVCIEDAFQRILAKAV 408

BLAST of Tan0013338 vs. ExPASy TrEMBL
Match: A0A5D3C6U2 (Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002990 PE=4 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.5e-182
Identity = 328/404 (81.19%), Postives = 365/404 (90.35%), Query Frame = 0

Query: 62  IMVKEKSSNERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSSPL 121
           + VKEK + ERKWKTTKV L++HI IP FP ELS YEYDAYFDEYATK  TKPFSQ+ PL
Sbjct: 1   MQVKEK-TEERKWKTTKVSLKDHICIPNFPTELSSYEYDAYFDEYATKTATKPFSQAIPL 60

Query: 122 WEIHIFNYPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQP 181
           WEIH+FNYPT+HA  +VI K+HHSIADG+CLMNTLLSCLKRADDPSLPLTFPSR+RS+QP
Sbjct: 61  WEIHVFNYPTTHATCSVIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQP 120

Query: 182 KNIPFFGHFSHLPS-LFSSISNFV----WSIMKNTFVEDDPTPIKPEGDSMQLVKPIAIS 241
           KN   F   SH P+  FSS+SNFV    WSIMKNTFVEDDPTPIKP+ DSMQLVKPIAIS
Sbjct: 121 KNKLDFCRLSHFPARFFSSVSNFVLNFGWSIMKNTFVEDDPTPIKPQEDSMQLVKPIAIS 180

Query: 242 TITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEHSPQSSEANSSALILLNTRKAK 301
           T+TFSLDQIKQIKNKLNASVNDVLTGIIFLG+RLYM+EH+P+SSEANSSALILLNTRKAK
Sbjct: 181 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGVRLYMQEHNPESSEANSSALILLNTRKAK 240

Query: 302 AYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSP-LSTCPLKFIKEAKQKIMLQRSPLS 361
           AYKSVKEMVK D+DAPWGNKIAFLPVPIPKLIDSP +S+ PL+F+++ K+KI LQRSPLS
Sbjct: 241 AYKSVKEMVKKDSDAPWGNKIAFLPVPIPKLIDSPVVSSTPLEFVEKVKEKITLQRSPLS 300

Query: 362 VFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALANHPVKGLYFTV 421
           VF+AAKVF+++K+VT  PE+GAKLFKRKLKNSSIMISN+IGPVEKMAL N PVKGLYFTV
Sbjct: 301 VFLAAKVFEILKNVTG-PEIGAKLFKRKLKNSSIMISNLIGPVEKMALVNRPVKGLYFTV 360

Query: 422 PGMPQSLMITIVSYMGDLRIVFGGEKCFIDHQKLKACIEDAYQR 460
           PGMPQSLMITIVSYMGDLRIVFGGEKCFI+ QKLK CIEDA+QR
Sbjct: 361 PGMPQSLMITIVSYMGDLRIVFGGEKCFINQQKLKVCIEDAFQR 402

BLAST of Tan0013338 vs. ExPASy TrEMBL
Match: A0A5A7SPR9 (Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G002950 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 3.2e-182
Identity = 327/404 (80.94%), Postives = 365/404 (90.35%), Query Frame = 0

Query: 62  IMVKEKSSNERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSSPL 121
           + VKEK + ERKWKTTKV L++HI IP FP ELS YEYDAYFDEY+TK  TKPFSQ+ PL
Sbjct: 1   MQVKEK-TEERKWKTTKVSLKDHICIPNFPTELSSYEYDAYFDEYSTKTATKPFSQAIPL 60

Query: 122 WEIHIFNYPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQP 181
           WEIH+FNYPT+HA  +VI K+HHSIADG+CLMNTLLSCLKRADDPSLPLTFPSR+RS+QP
Sbjct: 61  WEIHVFNYPTTHATCSVIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQP 120

Query: 182 KNIPFFGHFSHLPS-LFSSISNFV----WSIMKNTFVEDDPTPIKPEGDSMQLVKPIAIS 241
           KN   F   SH P+  FSS+SNFV    WSIMKNTFVEDDPTPIKP+ DSMQLVKPIAIS
Sbjct: 121 KNKLDFCRLSHFPARFFSSVSNFVLNFGWSIMKNTFVEDDPTPIKPQEDSMQLVKPIAIS 180

Query: 242 TITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEHSPQSSEANSSALILLNTRKAK 301
           T+TFSLDQIKQIKNKLNASVNDVLTGIIFLG+RLYM+EH+P+SSEANSSALILLNTRKAK
Sbjct: 181 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGVRLYMQEHNPESSEANSSALILLNTRKAK 240

Query: 302 AYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSP-LSTCPLKFIKEAKQKIMLQRSPLS 361
           AYKSVKEMVK D+DAPWGNKIAFLPVPIPKLIDSP +S+ PL+F+++ K+KI LQRSPLS
Sbjct: 241 AYKSVKEMVKKDSDAPWGNKIAFLPVPIPKLIDSPVVSSTPLEFVEKVKEKITLQRSPLS 300

Query: 362 VFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALANHPVKGLYFTV 421
           VF+AAKVF+++K+VT  PE+GAKLFKRKLKNSSIMISN+IGPVEKMAL N PVKGLYFTV
Sbjct: 301 VFLAAKVFEILKNVTG-PEIGAKLFKRKLKNSSIMISNLIGPVEKMALVNRPVKGLYFTV 360

Query: 422 PGMPQSLMITIVSYMGDLRIVFGGEKCFIDHQKLKACIEDAYQR 460
           PGMPQSLMITIVSYMGDLRIVFGGEKCFI+ QKLK CIEDA+QR
Sbjct: 361 PGMPQSLMITIVSYMGDLRIVFGGEKCFINQQKLKVCIEDAFQR 402

BLAST of Tan0013338 vs. TAIR 10
Match: AT3G49200.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 174.1 bits (440), Expect = 2.6e-43
Identity = 131/452 (28.98%), Postives = 223/452 (49.34%), Query Frame = 0

Query: 12  PMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSIITDVLIPANPRFTSIMVKEKSSN- 71
           P+SP ++ F+  E    ++ V+ L++ I     +  I   L+  +PRF+S +V   ++N 
Sbjct: 27  PLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLM-RHPRFSSKLVNNCNNNR 86

Query: 72  -ERKWKTTKVKLEEHIYIPKFPME-LSPYEYDAYFDEYATKIGTKPFSQSSPLWEIHIFN 131
            E+KW  T V +E+H+ IPK   + +     D + + Y + + T P   S PLWE+H+ +
Sbjct: 87  QEQKWVRTNVVVEDHVIIPKIQTQHIENANADVFLESYVSDLTTIPLDTSKPLWEVHLLD 146

Query: 132 YPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADD----PSLPL-TFPSRKRSQQPKN 191
             TS A++  +L+IHHS+ DG  +M+ +L+C ++  +    PSLP    PS   S    +
Sbjct: 147 LKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNRPSSGSSSLKTS 206

Query: 192 IPFFGHFSHLPSLFSSISNFVWS--------IMKNTFVEDDPTPIKPEGD-SMQLVKPIA 251
              +  F  L  +  S +  V +        I    F++D  TPIK  GD  +   K + 
Sbjct: 207 SRCYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPIK--GDFKLSKGKRMC 266

Query: 252 ISTITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEH--SPQSSEANSS------- 311
           +   T SLD IK IKN +  +VNDV+ G+   G+  Y++      + S+ NSS       
Sbjct: 267 MVHRTVSLDDIKLIKNAMKMTVNDVVLGVSQAGLSQYLKRRYGEQEESKRNSSNIPKGIR 326

Query: 312 --ALILLNTRKAKAYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSPLSTCPLKFIKEA 371
             A +L+N R     + + +M+   +   WGN I ++  P        L   PLK ++ A
Sbjct: 327 LRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYIIFP----FSIALCDDPLKHLRRA 386

Query: 372 KQKIMLQRSPLSVFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMAL 431
           K  I  +++ L   +   V  ++ + T   +  A +  R L N+++  SN++GPVE+++ 
Sbjct: 387 KSTIDRKKNSLEAVLTFVVGKILLN-TLGVQRAANVLNRALSNTTMSFSNLVGPVEEISF 446

Query: 432 ANHPVKGLYFTVPGMPQSLMITIVSYMGDLRI 436
             H V  +  +V G P +L +   SYM  L I
Sbjct: 447 YGHTVTYIAPSVYGHPHALTMHFQSYMNKLTI 470

BLAST of Tan0013338 vs. TAIR 10
Match: AT5G53390.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 172.6 bits (436), Expect = 7.7e-43
Identity = 121/438 (27.63%), Postives = 223/438 (50.91%), Query Frame = 0

Query: 12  PMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSII--TDVLIPANPRFTSIMVKEKSS 71
           P+SP S+ FN+ +   ++  ++ + S + + +  +II   +  +  +PRF+SI+ +  + 
Sbjct: 16  PLSPCSRLFNSPDFNCAI--IVTMGSKV-KGDTPAIIHGLEHTLVNHPRFSSIL-EMNNG 75

Query: 72  NERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSSPLWEIHIFNY 131
            + +W  TKVK+EEH+ +P    ++     D Y ++Y +K+ T P   S PLWE+H+   
Sbjct: 76  KKPRWVRTKVKVEEHVIVPDVDPDIE--NPDQYLEDYISKLTTIPMDLSKPLWEMHLLGV 135

Query: 132 PTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDP-SLPLTFPSRKRSQQPKNIPFF- 191
            TS+A+S  ILKIHHS+ DG  LM+ LL+C ++  DP +LP     +KR     N  FF 
Sbjct: 136 KTSNAESYAILKIHHSLGDGMSLMSLLLACTRKTSDPEALPTVAVHKKRFGPSCNSGFFN 195

Query: 192 -------GHFSHLPSLFSSISNFVWSIMKNTFVEDDPTPIKPEGDSMQLVKPIAISTITF 251
                  G +  L  LF++  + +   +    + D  TP+  +  S  + K      I+F
Sbjct: 196 KIWWLFVGLWFILRLLFNTFVDILMFALTIFVLRDTETPLLAKPGSELIPKRFVHRIISF 255

Query: 252 SLDQIKQIKNKLNASVNDVLTGIIFLGI-----RLYMEEHSPQSSEA----NSSALILLN 311
             D +K +KN +  +VNDVL G+   G+     R Y +E +P+S E+       + I++N
Sbjct: 256 --DDVKLVKNAMKMTVNDVLLGVTQAGLSRYLSRKYDQEATPKSKESMRRIRLRSAIMIN 315

Query: 312 TRKAKAYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSPLSTCPLKFIKEAKQKIMLQR 371
            R     +++ +M+   +   WGN   ++ +P        L T PL+++++AK  I  ++
Sbjct: 316 LRPNAGIEALADMMAKKSKCRWGNLFGYILLP----FSVGLETDPLEYVRQAKATIDRKK 375

Query: 372 SPLSVFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALANHPVKGL 430
             L    +   F ++  V    +    L ++ + ++++  SN++GP E++    HP+  +
Sbjct: 376 HSLEAVFSMAFFKLILKVLG-LKASVVLVRKVIHSTTLSFSNVVGPKEEITFHGHPLNYI 435

BLAST of Tan0013338 vs. TAIR 10
Match: AT3G49190.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 167.9 bits (424), Expect = 1.9e-41
Identity = 115/450 (25.56%), Postives = 217/450 (48.22%), Query Frame = 0

Query: 12  PMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSIITDVLIPANPRFTSIMVKEKSSNE 71
           P+SP ++ F+  E   +++ VI  +S +     +    +  I  +PRF+S +V +++   
Sbjct: 19  PLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKESFI-RHPRFSSKLVTDENGQN 78

Query: 72  RKWKTTKVKLEEHIYIPKF-PMELSPYEYDAYFDEYATKIGTKPFSQSSPLWEIHIFNYP 131
           ++W  T V +E+H  +PK  P  +     +A+ ++Y + +   P   S PLWE+H+ +  
Sbjct: 79  QRWVRTNVVVEDHFIVPKIKPQNIE--NSNAFLEDYVSDLMKIPLDTSRPLWELHLLDLK 138

Query: 132 TSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQQPKNIPFFGHF 191
           TS A++  +LKIHHS+ DG  +M+ +L+C+++  +P    + P + RS    ++   G  
Sbjct: 139 TSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLLTTGSR 198

Query: 192 SHLPSLF-------------SSISNFVWSIMKNTFVEDDPTPIKPEGDSMQL-VKPIAIS 251
           S    L+             +++ + +  I+   FV+D  TPIK  GD +    K + + 
Sbjct: 199 SDSRLLWLVKVIWTAVILGLNTVCDALEFIVTTLFVKDTETPIK--GDFLSTKSKQLRLV 258

Query: 252 TITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEHSPQSSEANSS----------- 311
             T SLD IK  KN +N ++NDV+ G+   G+  Y+     +    N             
Sbjct: 259 HRTVSLDDIKLTKNAMNMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKLPKRIRLR 318

Query: 312 ALILLNTRKAKAYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSPLSTCPLKFIKEAKQ 371
           + +L+N R     + + +M++  +   WGN   ++  P        L   PL+ ++ A++
Sbjct: 319 SALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGYVVFP----FSIALRDDPLEHLEIAQK 378

Query: 372 KIMLQRSPLSVFMAAKVFDMVKHVTADPEVGAKLFKRKLKNSSIMISNMIGPVEKMALAN 431
            I  +++     M   +F  +       +V A +  R + N+++  SNM+GPVE+++   
Sbjct: 379 TISRKKNSYGA-MLTYIFCRIIVKLLGIKVAASIINRMVSNTTMTFSNMVGPVEEVSFYG 438

Query: 432 HPVKGLYFTVPGMPQSLMITIVSYMGDLRI 436
           HP+     +  G P +L I   SYM  + I
Sbjct: 439 HPITYFASSAYGHPHALTIHCQSYMNKMTI 458

BLAST of Tan0013338 vs. TAIR 10
Match: AT3G49210.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 165.6 bits (418), Expect = 9.4e-41
Identity = 134/510 (26.27%), Postives = 233/510 (45.69%), Query Frame = 0

Query: 5   EDSTATTPMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSIITDVLIPANPRFTSIMV 64
           E+     P+SP ++ F+  E    V+ VI ++  I     +  +   LI  +PRF+S MV
Sbjct: 20  EEVEEEQPLSPAARVFHAPEFNCYVISVIGIKKKIDPDVIIEGLKQTLI-RHPRFSSKMV 79

Query: 65  KEKSSNERK----WKTTKVKLEEHIYIPKFPME-LSPYEYDAYFDEYATKIGTKPFSQSS 124
                N+++    W  T V + +H+ +     + +     DA+ + Y + + T P   S 
Sbjct: 80  STSVGNKKRQTQSWVRTNVVVTDHVIVSDIQTQNIENGNADAFLETYVSNLTTVPLDISK 139

Query: 125 PLWEIHIFNYPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSLPLTFPSRKRSQ 184
           PLW++H+ +  TS A++  +LK HHS+ DG  LM  +L+C+++  +P    + P++ RS 
Sbjct: 140 PLWQLHLLDLKTSDAENVAVLKFHHSLGDGMSLMALVLACMRKTSNPDELPSLPNQNRSS 199

Query: 185 QPKNIPFFGHFSHLPSLFSSISNFVWS---------------IMKNTFVEDDPTPIKPEG 244
              +    G  S   S F  +   +WS               I    F++D  TPIK  G
Sbjct: 200 SRSSRLMAG--SRGDSRFLWLVMVIWSAIMLVLNTVCDALEFIATTMFLKDTETPIK--G 259

Query: 245 D-SMQLVKPIAISTITFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMEEH-------- 304
           D      K + +   T SLD IK IKN +  +VNDV+ G+   G+  Y++          
Sbjct: 260 DFRFSKSKRMCLVHRTVSLDDIKLIKNTMKMTVNDVVLGVSQAGLSQYLDRRYGEKKKKV 319

Query: 305 -SPQSSEANSS---------ALILLNTRKAKAYKSVKEMVKSDTDAPWGNKIAFLPVPIP 364
              Q S+  ++         + +L+N R     + + +M+   +   WGN I ++  P  
Sbjct: 320 GEDQDSKRKATDMPKRIRLRSALLVNLRPNTGIQDLADMMAKGSTCRWGNWIGYIVFP-- 379

Query: 365 KLIDSPLSTCPLKFIKEAKQKIMLQRSPLSVFMAAKVFDMVKHV--TADPEVGAKLFKRK 424
                 L   PL+ ++ AK+ I  +++ L    AA  F   K +  T   +V AK+  R 
Sbjct: 380 --FSIGLRDDPLQHLRRAKRIIDRKKNSLE---AALTFVAGKFILKTFGVQVAAKIINRA 439

Query: 425 LKNSSIMISNMIGPVEKMALANHPVKGLYFTVPGMPQSLMITIVSYMGDLRIVFGGEKCF 462
           L N+++  SN+IGP+E+++   HP+  +  +V G P +L +   SYM  + I    +   
Sbjct: 440 LSNTTMSFSNLIGPIEEISFYGHPITYMAPSVYGHPHALTMHFQSYMNQMTISLTVDPTV 499

BLAST of Tan0013338 vs. TAIR 10
Match: AT5G12420.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 162.5 bits (410), Expect = 7.9e-40
Identity = 123/448 (27.46%), Postives = 218/448 (48.66%), Query Frame = 0

Query: 12  PMSPLSQYFNTSEMCVSVLGVIELESPITRYEAMSIITDVL---IPANPRFTSIMVKEKS 71
           P+SP+++ F +  + +  + ++  ++ I       ++ D L   +  +PRF+SI+    S
Sbjct: 8   PVSPMARVFQSPGIDLCAVTIMGFKTKIN----PDVVLDALKQNVSKHPRFSSIL----S 67

Query: 72  SNERKWKTTKVKLEEHIYIPKFPMELSPYEYDAYFDEYATKIGTKPFSQSSPLWEIHIFN 131
            N  KW  T+V +E+H+ +P    E       ++ D+Y +++   P  +S PLW+IHI N
Sbjct: 68  DNGAKWIETEVNVEDHVIVPYIDAEEIGEGGQSFIDDYMSRLTMIPLDRSRPLWDIHILN 127

Query: 132 YPTSHAKSNVILKIHHSIADGYCLMNTLLSCLKRADDPSL-PLTFPS-RKRSQQPKNIPF 191
             TS A++   ++ HHS+ DG  L++ +L+C  +  DP +     PS ++R+    ++  
Sbjct: 128 VKTSEAEAVGFIRSHHSLGDGMSLISLMLACTHKTSDPDMFSNAIPSMKRRATMSHSLKT 187

Query: 192 FGHFSHLPSLFSSISNFVWS--------IMKNTFVEDDPTPIKPEGDSMQLVKPIAISTI 251
            G F        S    +W+        +    F++D  TP+K   D      P      
Sbjct: 188 KGWFLRSIFTIGSTMRLLWNTTIDMLLLLATVLFLKDTKTPLKAGADVRS--NPKRFYHR 247

Query: 252 TFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYM--------EEHSPQSSEANS------ 311
             SLD IK IKN +N ++NDVL GI    +  Y+        EE    +S  N+      
Sbjct: 248 IISLDDIKLIKNAMNMTINDVLFGITQASLSHYLNRQYGTKKEEDGALTSYRNNLPDGIR 307

Query: 312 -SALILLNTRKAKAYKSVKEMVKSDTDAPWGNKIAFLPVPIPKLIDSPLSTCPLKFIKEA 371
                 +N R    +K + +M+  D+   WGN  +F+ +P        L T PL ++K +
Sbjct: 308 FRVACTVNLRSDIGFKPLADMMVKDSKCRWGNYFSFIFLP----FTIGLQTDPLVYLKMS 367

Query: 372 KQKIMLQRSPLSVFMAAKVFDMVKHV--TADPEVGAKLFKRKLKNSSIMISNMIGPVEKM 430
           K   M+ R   S + AA V+ ++K V      +  A+LF R ++N++  +SN+IGP+E++
Sbjct: 368 KS--MMARKKHS-YHAALVYFIIKIVLKVFGAKAAAELFDRPVRNTTTCVSNVIGPMEEI 427

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M3B23.7e-4228.98Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3... [more]
Q5KS411.1e-4127.63Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=... [more]
Q9M3B32.7e-4025.56Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B11.3e-3926.27Wax ester synthase/diacylglycerol acyltransferase 6 OS=Arabidopsis thaliana OX=3... [more]
Q94CK01.1e-3827.46Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
XP_022147882.19.9e-21882.23O-acyltransferase WSD1-like [Momordica charantia] >XP_022147883.1 O-acyltransfer... [more]
XP_011659709.24.7e-21281.57O-acyltransferase WSD1 [Cucumis sativus] >KGN43701.2 hypothetical protein Csa_01... [more]
XP_022952997.11.1e-20379.27O-acyltransferase WSD1-like [Cucurbita moschata][more]
XP_038887587.13.2e-18478.50O-acyltransferase WSD1-like isoform X2 [Benincasa hispida][more]
XP_038887586.15.5e-18478.69O-acyltransferase WSD1-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1D2I74.8e-21882.23Diacylglycerol O-acyltransferase OS=Momordica charantia OX=3673 GN=LOC111016711 ... [more]
A0A6J1GLZ35.1e-20479.27Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455512 P... [more]
A0A0A0K2E91.8e-18581.46Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051330 PE=4... [more]
A0A5D3C6U21.5e-18281.19Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7SPR93.2e-18280.94Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
Match NameE-valueIdentityDescription
AT3G49200.12.6e-4328.98O-acyltransferase (WSD1-like) family protein [more]
AT5G53390.17.7e-4327.63O-acyltransferase (WSD1-like) family protein [more]
AT3G49190.11.9e-4125.56O-acyltransferase (WSD1-like) family protein [more]
AT3G49210.19.4e-4126.27O-acyltransferase (WSD1-like) family protein [more]
AT5G12420.17.9e-4027.46O-acyltransferase (WSD1-like) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004255O-acyltransferase, WSD1, N-terminalPFAMPF03007WES_acyltransfcoord: 103..259
e-value: 2.6E-8
score: 34.0
IPR009721O-acyltransferase WSD1, C-terminalPFAMPF06974WS_DGAT_Ccoord: 313..458
e-value: 3.6E-31
score: 108.1
IPR045034O-acyltransferase WSD1-likePANTHERPTHR31650O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEINcoord: 9..464
NoneNo IPR availablePANTHERPTHR31650:SF34O-ACYLTRANSFERASE WSD1-LIKEcoord: 9..464

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0013338.1Tan0013338.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019432 triglyceride biosynthetic process
biological_process GO:0045017 glycerolipid biosynthetic process
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004144 diacylglycerol O-acyltransferase activity
molecular_function GO:0047196 long-chain-alcohol O-fatty-acyltransferase activity
molecular_function GO:0008374 O-acyltransferase activity