Tan0013285 (gene) Snake gourd v1

BLAST of Tan0013285 vs. ExPASy Swiss-Prot
Match: A0A0S2IHL2 (Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 2.2e-168
Identity = 294/526 (55.89%), Postives = 391/526 (74.33%), Query Frame = 0

Query: 3   IAAAISISILVGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATAI 62
           IAAA++++++V   + W W+ LNW+W+ P+KLE+ LR+QG  G+SYRL +GD K+ +   
Sbjct: 11  IAAAVAVAVVV---VGWAWKVLNWVWVSPRKLEESLRKQGFRGNSYRLFYGDLKESSEMT 70

Query: 63  REARSQPINFSHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVF---SL 122
           R+A+ +PIN S D   R     H T+ KYGK+ F+W+GPTPRV I DPE +K +      
Sbjct: 71  RKAKLKPINLSDDPVLRVRPFIHQTVKKYGKSSFIWIGPTPRVQIMDPEIIKEIMVKSYK 130

Query: 123 FNDIRKAPLFPIARRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMI 182
           FN  ++ PL     +L + GL + EG  W+KHRK++NPAFH ++LK M PA   SC EM+
Sbjct: 131 FNKPKRNPLV----KLFADGLANHEGELWAKHRKLLNPAFHLERLKCMLPAMYFSCIEMV 190

Query: 183 KKWESMVCESEELGCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVT 242
            KW+ M+ +    G  ELDVWP LQ +T+DVIS TAFGSSY+EG  +F+ L+   + LV 
Sbjct: 191 SKWDKMISKD---GSRELDVWPFLQRLTSDVISHTAFGSSYEEGNIVFE-LQTEQAELVM 250

Query: 243 RLTKGVYNIPGWRFVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGIL 302
           +  + VY IPGW ++PTK NRKM+E+DR+ Q+ +  II+K+  AM+AGE S + D+LGIL
Sbjct: 251 KTLQSVY-IPGWSYLPTKRNRKMKEIDRKTQSCLMNIINKKTKAMQAGEGSTD-DILGIL 310

Query: 303 LESNLNQVQEEQENKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDR 362
           LESNL   ++  + K +VGMS+++++ ECK FYFAGQETT+ LLVWTMVLLS +  WQ R
Sbjct: 311 LESNLK--EQLGQGKKNVGMSIQEVMGECKQFYFAGQETTSGLLVWTMVLLSIHPNWQAR 370

Query: 363 ARAEVLEVFGDDNKLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPA 422
           AR EVL+ FG + K DFD L+ LK+VTMIL EVLRLYPP+  L R +D+ET LG++ LPA
Sbjct: 371 AREEVLQQFG-NAKPDFDNLNHLKIVTMILYEVLRLYPPVDTLFRRVDQETTLGDITLPA 430

Query: 423 EVSIGIPIIHIHHDRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFA 482
            V I +PI+ +HHD+ IWG+DA EF PERFSEG+SKATKNQV F PFGWGPRIC+GQNFA
Sbjct: 431 GVQISLPIMILHHDQNIWGDDAKEFNPERFSEGVSKATKNQVVFFPFGWGPRICIGQNFA 490

Query: 483 MIEAKIALSMILQRFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           ++EAK+AL++ILQRFSFELSPSYTHAPT+ +TVQPQHGA+LIL +L
Sbjct: 491 LLEAKLALAIILQRFSFELSPSYTHAPTTVLTVQPQHGANLILHKL 520

BLAST of Tan0013285 vs. ExPASy Swiss-Prot
Match: H2DH21 (Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 1.1e-162
Identity = 279/506 (55.14%), Postives = 374/506 (73.91%), Query Frame = 0

Query: 20  GWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATAIREARSQPINFSHDIAHR 79
           GWR  NW+WL+P+KLEK LR QG  G+SYRL FGD K+M   ++EA+S+PIN   DI  R
Sbjct: 21  GWRIFNWVWLRPRKLEKYLRNQGFNGNSYRLFFGDVKEMIVMLKEAKSKPINLYDDIIPR 80

Query: 80  AVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFNDIRKAPLFPIARRLGSGG 139
            +  +   I  YGKN F+W+GP P V+I +P+ +K V S F   +K    P+ + L + G
Sbjct: 81  IIPLNQKIITNYGKNSFLWLGPKPMVHIMNPDHIKDVLSKFYQFQKPRHNPLTKLLAT-G 140

Query: 140 LVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKKWESMVCESEELGCFELDV 199
           +   EG +W+KHRK+INPAFH +KLK+M PA   S +E++ KWE MV      G FELDV
Sbjct: 141 VADAEGDRWAKHRKLINPAFHLEKLKNMLPAIYLSSSEIVTKWEEMVSTK---GQFELDV 200

Query: 200 WPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRLTKGVYNIPGWRFVPTKLN 259
            P L+ +T+DVISRTAFGSSY+EG+KIFQ  +    L++ + ++ +Y +PG RF+PTK N
Sbjct: 201 LPYLETLTSDVISRTAFGSSYEEGRKIFQLQREQAELII-QASQTIY-LPGMRFLPTKRN 260

Query: 260 RKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLESNLNQVQEEQENKNDVGM 319
           ++M+E+ +E++  +  II+KR  AMEAGE S++ DLLGILLESN  ++  +Q    + G+
Sbjct: 261 KRMKEIAKEVKIALKSIINKRLKAMEAGERSSHDDLLGILLESNSKEI--KQHGNTNFGL 320

Query: 320 SMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRARAEVLEVFGDDNKLDFDGL 379
           +++++I ECKLF+FAGQETT+ LLVWTM+LLS++ +WQ RA+ EVL  FG +NK DFDGL
Sbjct: 321 TVDEVIEECKLFFFAGQETTSNLLVWTMILLSQHQDWQKRAKEEVLRTFG-NNKPDFDGL 380

Query: 380 SRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEVSIGIPIIHIHHDRQIWGE 439
           + LKVV MIL EVLRLYPPI  L R I +E KLG + LPA V + +PII +H+D++IWG+
Sbjct: 381 NHLKVVNMILLEVLRLYPPILSLDRTIYEEIKLGEISLPAGVILLLPIILLHYDQEIWGD 440

Query: 440 DATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMIEAKIALSMILQRFSFELS 499
           DA EF PERFSEG+ KATK +V + PF WGPRIC+GQNFAM+EAK+A++MILQRFSF LS
Sbjct: 441 DAKEFNPERFSEGVLKATKGRVTYFPFSWGPRICIGQNFAMLEAKMAMAMILQRFSFVLS 500

Query: 500 PSYTHAPTSNVTVQPQHGAHLILRQL 526
           PSY HAP + +T+QPQ+GAHLIL  L
Sbjct: 501 PSYAHAPHAIITLQPQYGAHLILHSL 517

BLAST of Tan0013285 vs. ExPASy Swiss-Prot
Match: W8JWW3 (Cytochrome P450 72A225 OS=Catharanthus roseus OX=4058 GN=CYP72A225 PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 2.5e-159
Identity = 279/508 (54.92%), Postives = 373/508 (73.43%), Query Frame = 0

Query: 17  LAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATAIREARSQPINFSHDI 76
           L   W+ LNW W+KPKKLEK LRQ GL G+SYRLL GD  + +   +EA+S+PINF++ I
Sbjct: 34  LLLAWKMLNWAWIKPKKLEKRLRQLGLKGNSYRLLIGDLGETSQMDKEAKSKPINFTNHI 93

Query: 77  AHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFNDIRKAPLFPIARRLG 136
             R +   H  I  +GKN F+W GP P V ITDPE +K V +     +K P   +  +L 
Sbjct: 94  IPRIMPFLHKIIQSHGKNPFIWQGPNPAVIITDPELIKEVLTKNYTYQKPPSDDLG-KLI 153

Query: 137 SGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKKWESMVCESEELGCFE 196
           S G+ + +  KW+KHR+++NPAFH +KLK M PAF  SC+EM+KKWE +   S E G FE
Sbjct: 154 SQGVAAYDKDKWAKHRRLLNPAFHIEKLKHMLPAFHLSCSEMLKKWEKLASASAE-GSFE 213

Query: 197 LDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRLTKGVYNIPGWRFVPT 256
           LDVWP LQ+MT+DVISRTAFGS+ +EGK IF+  K  +  ++  L    +  PG  ++PT
Sbjct: 214 LDVWPYLQDMTSDVISRTAFGSNLEEGKNIFKLQKEILEHILQILQS--FYFPGMLYLPT 273

Query: 257 KLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLESNLNQVQEEQENKN- 316
           K  R+M+++ +E+ + V  II KR  AM+AGE SN+ DLLGILL+SN  +++ +  N + 
Sbjct: 274 KRIRRMKKISKEVHSSVLNIIMKRVEAMKAGEPSND-DLLGILLDSNFKEIENDNGNNSN 333

Query: 317 -DVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRARAEVLEVFGDDNKL 376
            + GMS E++I ECKLFYFAGQETTA LLVWT++LLS++ EWQ RAR EVL++FG +++ 
Sbjct: 334 KNCGMSFEEVIEECKLFYFAGQETTAVLLVWTLLLLSKHPEWQMRARDEVLQIFG-NSEP 393

Query: 377 DFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEVSIGIPIIHIHHDR 436
           DFD L+ LKV+TM++NEVLRLYPP  +++R I ++TKLG+L LP  +SI +P I +HHD 
Sbjct: 394 DFDALNHLKVITMVINEVLRLYPPGFMMSRVIHEDTKLGSLSLPGGISIFLPTIMLHHDE 453

Query: 437 QIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMIEAKIALSMILQRF 496
           ++WG+DA EFKPERFSEGISKATK Q  + PF  GPRIC+G NFAM+EAK+AL++ILQRF
Sbjct: 454 ELWGDDAKEFKPERFSEGISKATKGQFIYFPFSGGPRICIGNNFAMLEAKMALALILQRF 513

Query: 497 SFELSPSYTHAPTSNVTVQPQHGAHLIL 523
           +FE+SPSYTHAP + VT+QPQ+GA LIL
Sbjct: 514 TFEISPSYTHAPHTLVTLQPQYGAQLIL 535

BLAST of Tan0013285 vs. ExPASy Swiss-Prot
Match: Q2MJ19 (Cytochrome P450 72A68 OS=Medicago truncatula OX=3880 GN=CYP72A68 PE=1 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 1.6e-158
Identity = 274/524 (52.29%), Postives = 386/524 (73.66%), Query Frame = 0

Query: 2   TIAAAISISILVGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATA 61
           T +A I I++  G  L + WR LNW+WLKPKK+EKLLR+QGL G+ YRLL GD+KD    
Sbjct: 7   TKSAIILITVTFG--LVYAWRVLNWMWLKPKKIEKLLREQGLQGNPYRLLLGDAKDYFVM 66

Query: 62  IREARSQPINFSHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFN 121
            ++ +S+P+N S DIA R     H  +  +GK  F+W G  P V + +PEQ++ VF+  +
Sbjct: 67  QKKVQSKPMNLSDDIAPRVAPYIHHAVQTHGKKSFIWFGMKPWVILNEPEQIREVFNKMS 126

Query: 122 DIRKAPLFPIARRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKK 181
           +  K        +L + GLV LEG KWSKHR+IINPAFH +KLK M P FL+SCN++I  
Sbjct: 127 EFPKVQY--KFMKLITRGLVKLEGEKWSKHRRIINPAFHMEKLKIMTPTFLKSCNDLISN 186

Query: 182 WESMVCESEELGCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRL 241
           WE M+  +   G  E+DVWP LQ++T+DVI+R++FGSSY+EG+K+FQ       L++  L
Sbjct: 187 WEKMLSSN---GSCEMDVWPSLQSLTSDVIARSSFGSSYEEGRKVFQLQIEQGELIMKNL 246

Query: 242 TKGVYNIPGWRFVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLE 301
            K +  IP WRF+PT  +RK+ E +++I+T +  II+KR+ A++AGEA+ N DLLG+LLE
Sbjct: 247 MKSL--IPLWRFLPTADHRKINENEKQIETTLKNIINKREKAIKAGEATEN-DLLGLLLE 306

Query: 302 SNLNQVQEEQENKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRAR 361
           SN  +++E    KN +G+S+E+++ EC+LF+ AGQETT+ LLVWTMVLLSRY +WQ+RAR
Sbjct: 307 SNHREIKEHGNVKN-MGLSLEEVVGECRLFHVAGQETTSDLLVWTMVLLSRYPDWQERAR 366

Query: 362 AEVLEVFGDDNKLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEV 421
            EVLE+FG++ K DFDGL++LK++ MIL EVLRLYPP+  +AR ++ + KLG+L L A +
Sbjct: 367 KEVLEIFGNE-KPDFDGLNKLKIMAMILYEVLRLYPPVTGVARKVENDIKLGDLTLYAGM 426

Query: 422 SIGIPIIHIHHDRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMI 481
            + +PI+ IHHD ++WG+DA  F PERFS GISKAT  +  + PFG GPRIC+GQNF+++
Sbjct: 427 EVYMPIVLIHHDCELWGDDAKIFNPERFSGGISKATNGRFSYFPFGAGPRICIGQNFSLL 486

Query: 482 EAKIALSMILQRFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           EAK+A+++IL+ FSFELS +Y HAP+  ++VQPQHGAH+ILR++
Sbjct: 487 EAKMAMALILKNFSFELSQTYAHAPSVVLSVQPQHGAHVILRKI 518

BLAST of Tan0013285 vs. ExPASy Swiss-Prot
Match: H1A988 (11-oxo-beta-amyrin 30-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP72A154 PE=1 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 2.1e-158
Identity = 285/522 (54.60%), Postives = 373/522 (71.46%), Query Frame = 0

Query: 7   ISISILVGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKD--MATAIRE 66
           + +++++ ++  W    +N +WL+PK+LE+ LR QGL G  Y+L   +SK   M    +E
Sbjct: 12  VVLTVILAAIPIWVCHMVNTLWLRPKRLERHLRAQGLHGDPYKLSLDNSKQTYMLKLQQE 71

Query: 67  ARSQPINFS-HDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFNDI 126
           A+S+ I  S  D A R  + +H T++KYGKN F W G  P+V ITDPEQ+K VF+   D 
Sbjct: 72  AQSKSIGLSKDDAAPRIFSLAHQTVHKYGKNSFAWEGTAPKVIITDPEQIKEVFNKIQDF 131

Query: 127 RKAPLFPIARRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKKWE 186
            K  L PIA+ + S GLV  EG KW+KHRKIINPAFH +KLK M PAF  SC+EMI KW+
Sbjct: 132 PKPKLNPIAKYI-SIGLVQYEGDKWAKHRKIINPAFHLEKLKGMLPAFSHSCHEMISKWK 191

Query: 187 SMVCESEELGCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRLTK 246
            ++      G  E+DVWP LQN+T DVISRTAFGSSY EG KIF+ LK     L   +T 
Sbjct: 192 GLLSSD---GTCEVDVWPFLQNLTCDVISRTAFGSSYAEGAKIFELLKRQGYAL---MTA 251

Query: 247 GVYNIPGWRFVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLESN 306
               IP W  +P+   R+M+E++R I+  + GII KR+ A+++G+++++ DLLGILL+SN
Sbjct: 252 RYARIPLWWLLPSTTKRRMKEIERGIRDSLEGIIRKREKALKSGKSTDD-DLLGILLQSN 311

Query: 307 LNQVQEEQENKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRARAE 366
            +   +  EN    GM+ ++++ ECKLFY AGQETTAALL WTMVLL ++ EWQ RAR E
Sbjct: 312 -HIENKGDENSKSAGMTTQEVMEECKLFYLAGQETTAALLAWTMVLLGKHPEWQARARQE 371

Query: 367 VLEVFGDDNKLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEVSI 426
           VL+VFG+ N  +F+GL RLK+VTMIL EVLRLYPP   L RA+ K+ KLGNLLLPA V +
Sbjct: 372 VLQVFGNQNP-NFEGLGRLKIVTMILYEVLRLYPPGIYLTRALRKDLKLGNLLLPAGVQV 431

Query: 427 GIPIIHIHHDRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMIEA 486
            +PI+ IHHD  IWG DA EF PERF+EGI+KATK QVC+ PFGWGPRICVGQNFA++EA
Sbjct: 432 SVPILLIHHDEGIWGNDAKEFNPERFAEGIAKATKGQVCYFPFGWGPRICVGQNFALLEA 491

Query: 487 KIALSMILQRFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           KI LS++LQ FSFELSP+Y H PT+ +T+QP+HGA +IL +L
Sbjct: 492 KIVLSLLLQNFSFELSPTYAHVPTTVLTLQPKHGAPIILHKL 523

BLAST of Tan0013285 vs. NCBI nr
Match: XP_023524668.1 (cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 902.9 bits (2332), Expect = 1.3e-258
Identity = 440/525 (83.81%), Postives = 471/525 (89.71%), Query Frame = 0

Query: 1   MTIAAAISISILVGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMAT 60
           MT+ AAI+I I+ GSLL WGW FLNWIWLKPKKLEK LR+QGLAG+ YRLLFGDSKDMAT
Sbjct: 3   MTMGAAITIIIVAGSLLLWGWSFLNWIWLKPKKLEKQLREQGLAGNPYRLLFGDSKDMAT 62

Query: 61  AIREARSQPINFSHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLF 120
           AIREARSQ INFSH IAHRA+ SSH TINKYGKNCF WVGPTPRVYITDPEQLKTVFSL 
Sbjct: 63  AIREARSQSINFSHHIAHRALPSSHLTINKYGKNCFAWVGPTPRVYITDPEQLKTVFSLI 122

Query: 121 NDIRKAPLFPIARRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIK 180
           NDI KAPLFP AR  GSGGLVSLEG KW++HRKIINPAFH+QKLKDMFPAF ESCNEMI 
Sbjct: 123 NDIPKAPLFPPARTPGSGGLVSLEGSKWAQHRKIINPAFHTQKLKDMFPAFFESCNEMIN 182

Query: 181 KWESMVCESEELGCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTR 240
           KWE MV E EE GCFELDVWPDLQNMTAD ISRTAFGSSY+EGKKIFQFLK WVSLLV R
Sbjct: 183 KWEVMVSEEEE-GCFELDVWPDLQNMTADSISRTAFGSSYEEGKKIFQFLKTWVSLLVAR 242

Query: 241 LTKGVYNIPGWRFVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILL 300
           LTKGVYNIPGWRFVPT++NRKMEEV+REI++MVWGIISKRQ AMEA + SNNADLLGILL
Sbjct: 243 LTKGVYNIPGWRFVPTQMNRKMEEVNREIKSMVWGIISKRQAAMEAAQVSNNADLLGILL 302

Query: 301 ESNLNQVQEEQENKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRA 360
           E N          K+ VGMSMEDIISECKLFYFAGQETTA+LLVWTMVLL RYSEWQDRA
Sbjct: 303 EEN---------KKDAVGMSMEDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDRA 362

Query: 361 RAEVLEVFGDDNKLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAE 420
           RAEVL+VFG+D K+DFDGL+RLKVV+MIL EVLRLYPP+GILAR I KETKLGNL+LP  
Sbjct: 363 RAEVLDVFGEDKKVDFDGLNRLKVVSMILYEVLRLYPPVGILARGIHKETKLGNLILPGG 422

Query: 421 VSIGIPIIHIHHDRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAM 480
           VS+GIPIIHIHHD QIWGEDA EFKPERFSEGISKAT N+VCF+PFGWGPRICVGQNFAM
Sbjct: 423 VSLGIPIIHIHHDHQIWGEDADEFKPERFSEGISKATNNKVCFLPFGWGPRICVGQNFAM 482

Query: 481 IEAKIALSMILQRFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           IEAKIALSMILQ FSF+LSPSYTH+P SNVTVQPQHGAHLILR+L
Sbjct: 483 IEAKIALSMILQSFSFQLSPSYTHSPASNVTVQPQHGAHLILRKL 517

BLAST of Tan0013285 vs. NCBI nr
Match: XP_022941520.1 (cytochrome P450 CYP72A219-like [Cucurbita moschata])

HSP 1 Score: 897.9 bits (2319), Expect = 4.2e-257
Identity = 439/525 (83.62%), Postives = 470/525 (89.52%), Query Frame = 0

Query: 1   MTIAAAISISILVGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMAT 60
           MT+  AI+I I+ GSLL WGW FLNWIWLKPKKLEK LR+QGLAG+ YRLLFGDSKDMAT
Sbjct: 3   MTMGGAITIIIVAGSLLVWGWSFLNWIWLKPKKLEKQLREQGLAGNPYRLLFGDSKDMAT 62

Query: 61  AIREARSQPINFSHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLF 120
           AIREARSQ +NFSH IAHRA+ SSH TINKYGKNCF WVGPTPRVYIT PEQLKTVFSL 
Sbjct: 63  AIREARSQSMNFSHHIAHRALPSSHLTINKYGKNCFAWVGPTPRVYITHPEQLKTVFSLI 122

Query: 121 NDIRKAPLFPIARRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIK 180
           NDI KAPLFP AR  GSGGLVSLEG KW+KHRKIINPAFH++KLKDMFPAF ESCNEMIK
Sbjct: 123 NDIPKAPLFPPARTPGSGGLVSLEGSKWAKHRKIINPAFHTEKLKDMFPAFFESCNEMIK 182

Query: 181 KWESMVCESEELGCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTR 240
           KWE MV E EE G FELDVWPDLQNMTAD ISRTAFGSSY+EGKKIFQFLK WVSLLV R
Sbjct: 183 KWEVMVSEEEE-GRFELDVWPDLQNMTADSISRTAFGSSYEEGKKIFQFLKTWVSLLVAR 242

Query: 241 LTKGVYNIPGWRFVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILL 300
           LTKGVYNIPGWRFVPT++NRKMEEV+REI++MVWGIISKRQTAMEA + SNNADLLGILL
Sbjct: 243 LTKGVYNIPGWRFVPTQMNRKMEEVNREIKSMVWGIISKRQTAMEAAQVSNNADLLGILL 302

Query: 301 ESNLNQVQEEQENKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRA 360
           E N          K+ VGMSMEDIISECKLFYFAGQETTA+LLVWTMVLL RYSEWQD+A
Sbjct: 303 EEN---------KKDAVGMSMEDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQA 362

Query: 361 RAEVLEVFGDDNKLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAE 420
           RAEVL+VFG+D K+DFDGL+RLKVV+MIL EVLRLYPP+GILAR I KETKLGNLLLP  
Sbjct: 363 RAEVLDVFGEDKKVDFDGLNRLKVVSMILYEVLRLYPPVGILARGIHKETKLGNLLLPGG 422

Query: 421 VSIGIPIIHIHHDRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAM 480
           VSIGIPIIHIHHD QIWGEDA EFKPERFSEGISKAT N+VCF+PFGWGPRICVGQNFAM
Sbjct: 423 VSIGIPIIHIHHDHQIWGEDADEFKPERFSEGISKATNNKVCFLPFGWGPRICVGQNFAM 482

Query: 481 IEAKIALSMILQRFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           IEAKIAL+MILQ FSF+LSPSYTHAP SNVTVQPQHGAHLILR+L
Sbjct: 483 IEAKIALTMILQHFSFQLSPSYTHAPASNVTVQPQHGAHLILRKL 517

BLAST of Tan0013285 vs. NCBI nr
Match: XP_023006210.1 (cytochrome P450 CYP72A219-like [Cucurbita maxima])

HSP 1 Score: 807.0 bits (2083), Expect = 9.7e-230
Identity = 389/513 (75.83%), Postives = 444/513 (86.55%), Query Frame = 0

Query: 13  VGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATAIREARSQPINF 72
           +G L  WGW+ LNW+W+ PK+LEK LRQQGLAG+SYRLLFGDSKD A A+R+AR+QPI+F
Sbjct: 10  LGLLGVWGWKILNWMWVTPKRLEKCLRQQGLAGNSYRLLFGDSKDTAAAVRQARTQPISF 69

Query: 73  SHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFNDIRKAPLFPIA 132
           SH IA RA  SSH TI++YGK+ F W+GP+PRVYIT PEQ+K VFS  NDIRKA  FP  
Sbjct: 70  SHRIAARATPSSHITIDQYGKDSFTWIGPSPRVYITQPEQVKMVFSQINDIRKATSFPFR 129

Query: 133 RRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKKWESMVCESEEL 192
           RR+G GG+VSLEG KW+KHRKIINPAFH +KLKDMFPAF +SC+EMI KWE M+ +    
Sbjct: 130 RRIG-GGVVSLEGAKWAKHRKIINPAFHMEKLKDMFPAFSKSCSEMINKWEMMITKE--- 189

Query: 193 GCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRLTKGVYNIPGWR 252
           GC ELDVWPDLQNM ADVISRTAFGSSY+EGKKIFQ LK W SLL+  + KGVY +PG R
Sbjct: 190 GCCELDVWPDLQNMAADVISRTAFGSSYEEGKKIFQLLKQWASLLMAYVMKGVYFLPGLR 249

Query: 253 FVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLESNLNQVQEEQE 312
           F+PTKLN++M+E++ +I+ M+WGIISKRQ AM+ GEASN+ DLLGILLESN  ++Q E +
Sbjct: 250 FLPTKLNKRMDEINGKIRDMIWGIISKRQNAMKKGEASND-DLLGILLESNSREIQ-EHK 309

Query: 313 NKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRARAEVLEVFGDDN 372
           NK DVGMS+E++I+EC+LFYFAGQETTAALL WTMVLL RYSEWQDRARAEVLEVFGD+ 
Sbjct: 310 NKKDVGMSIEEVIAECRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEVFGDNK 369

Query: 373 KLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEVSIGIPIIHIHH 432
           KLDFDGLSRLKVVTMILNEVLRLYPP+G+LAR I KETKLGNL LP  VSIGIPI+ IH 
Sbjct: 370 KLDFDGLSRLKVVTMILNEVLRLYPPVGMLAREIHKETKLGNLTLPVGVSIGIPIVCIHQ 429

Query: 433 DRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMIEAKIALSMILQ 492
           D  IWGEDA+EFKPERF+EGISKATKNQVCFIPFGWGPRIC+GQNFAMIEAKIALSMILQ
Sbjct: 430 DPTIWGEDASEFKPERFAEGISKATKNQVCFIPFGWGPRICLGQNFAMIEAKIALSMILQ 489

Query: 493 RFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           RFSFELSPSYTHAP SNVT+QPQHGAHLIL  L
Sbjct: 490 RFSFELSPSYTHAPISNVTIQPQHGAHLILHML 516

BLAST of Tan0013285 vs. NCBI nr
Match: XP_038904026.1 (cytochrome P450 CYP72A219-like [Benincasa hispida])

HSP 1 Score: 806.2 bits (2081), Expect = 1.7e-229
Identity = 381/507 (75.15%), Postives = 438/507 (86.39%), Query Frame = 0

Query: 19  WGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATAIREARSQPINFSHDIAH 78
           WGWR +NW+W +PK+LEK LR QGLAG+SYRLLFGD+K++A A+ +ARS  ++FSHDIA 
Sbjct: 18  WGWRVVNWVWFRPKRLEKCLRHQGLAGNSYRLLFGDTKEIAAAVHQARSHSMSFSHDIAP 77

Query: 79  RAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFNDIRKAPLFPIARRLGSG 138
           R   +SH TI+KYGK+ F W+G TPRVYIT+PEQ+K VFS  NDIRK   FP+ RR GSG
Sbjct: 78  RTTPTSHTTIHKYGKDSFTWIGTTPRVYITEPEQVKIVFSQINDIRKTSSFPLRRRKGSG 137

Query: 139 GLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKKWESMVCESEELGCFELD 198
           G+VSLEG KW+KHRKIINPAFH +KLKDMFPAF +SC EM+ KWE M+   E  G  E+D
Sbjct: 138 GVVSLEGSKWAKHRKIINPAFHMEKLKDMFPAFSKSCREMVNKWEKMIPAEE--GSCEID 197

Query: 199 VWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRLTKGVYNIPGWRFVPTKL 258
           VWPDLQNM ADVISRTAFGSS++EGKKIFQ LK W  LL+T LTKGVY IPG+R++PTKL
Sbjct: 198 VWPDLQNMAADVISRTAFGSSFEEGKKIFQLLKQWAMLLMTYLTKGVYFIPGFRYMPTKL 257

Query: 259 NRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLESNLNQVQEEQENKNDVG 318
           N++MEE+DR+IQ MVWGII KRQ AM+ GE SNN DLLGILLESN +Q+ EE +NK DVG
Sbjct: 258 NKRMEEIDRKIQDMVWGIIRKRQNAMKKGEGSNNEDLLGILLESNASQI-EEHKNKKDVG 317

Query: 319 MSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRARAEVLEVFGDDNKLDFDG 378
           MS+E++ISEC+LFYFAGQETTAALL WTMVLL+RYSEWQDRARAEVLEVFGD NKLDFDG
Sbjct: 318 MSIEEVISECRLFYFAGQETTAALLAWTMVLLARYSEWQDRARAEVLEVFGDSNKLDFDG 377

Query: 379 LSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEVSIGIPIIHIHHDRQIWG 438
           LSRL+VV MILNEVLRLYPP+G+LAR I  ETKLGNL LP  VSIGIPI+ IH + +IWG
Sbjct: 378 LSRLRVVNMILNEVLRLYPPVGMLAREIHNETKLGNLTLPVGVSIGIPILSIHQNTKIWG 437

Query: 439 EDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMIEAKIALSMILQRFSFEL 498
           EDA EFKPERFSEGISKATKNQVCFIPFGWGPRIC+GQNFAMIEAKIALSMILQ+FSF L
Sbjct: 438 EDAIEFKPERFSEGISKATKNQVCFIPFGWGPRICLGQNFAMIEAKIALSMILQQFSFTL 497

Query: 499 SPSYTHAPTSNVTVQPQHGAHLILRQL 526
           SP+YTHAP SN+T+QPQHGAHLILR+L
Sbjct: 498 SPAYTHAPISNITIQPQHGAHLILRKL 521

BLAST of Tan0013285 vs. NCBI nr
Match: XP_022924700.1 (cytochrome P450 CYP72A219-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 806.2 bits (2081), Expect = 1.7e-229
Identity = 386/513 (75.24%), Postives = 442/513 (86.16%), Query Frame = 0

Query: 13  VGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATAIREARSQPINF 72
           +G L  WGW+ LNW+WL PK+LEK LR QGLAG+SYRLLFGDSKD A A+R+A++QPI+F
Sbjct: 32  LGLLGVWGWKILNWMWLTPKRLEKCLRHQGLAGNSYRLLFGDSKDTAAAVRQAKTQPISF 91

Query: 73  SHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFNDIRKAPLFPIA 132
           SH IA RA  SSH TI++YGK+ F W+GPTPRVYIT PEQ+K VFS  NDIRKA LFP  
Sbjct: 92  SHRIAARATPSSHITIHRYGKDSFTWIGPTPRVYITHPEQVKVVFSQINDIRKATLFPFR 151

Query: 133 RRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKKWESMVCESEEL 192
           RR+G GG+VSLEG KW+KHRKIINPAFH +KLKDMFPAF +SC+EMI KWE M+ +    
Sbjct: 152 RRMG-GGVVSLEGAKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMINKWEMMITKE--- 211

Query: 193 GCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRLTKGVYNIPGWR 252
           GC ELDVWPDLQNM ADVISRTAFGSSY+EGKKIFQ LK W SLL+  + KGVY IPG R
Sbjct: 212 GCCELDVWPDLQNMAADVISRTAFGSSYEEGKKIFQLLKQWASLLMAYVMKGVYFIPGLR 271

Query: 253 FVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLESNLNQVQEEQE 312
           F+PTKLN++M+E++  I+ M+WGII+KRQ AM+ GEASN+ DLLGILLESN  ++Q E +
Sbjct: 272 FLPTKLNKRMDEINGNIRDMIWGIINKRQNAMKKGEASND-DLLGILLESNSREIQ-EHK 331

Query: 313 NKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRARAEVLEVFGDDN 372
           NK DVGMSME++I+EC+LFYFAGQETTAALL WTMVLL RYSEWQDRARAEVLE+FGD+ 
Sbjct: 332 NKKDVGMSMEEVIAECRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEIFGDNK 391

Query: 373 KLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEVSIGIPIIHIHH 432
           K DFDGLSRLK+VTMILNEVLRLYPP+G+L+R I KETKLGNL LP  VSIGIPI+ IH 
Sbjct: 392 KFDFDGLSRLKIVTMILNEVLRLYPPVGLLSREIHKETKLGNLTLPVGVSIGIPIVCIHQ 451

Query: 433 DRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMIEAKIALSMILQ 492
           D  +WGEDA+EFKPERF EGISKATKNQVCFIPFGWGPRIC+GQNFAMIEAKIALSMILQ
Sbjct: 452 DPTLWGEDASEFKPERFVEGISKATKNQVCFIPFGWGPRICLGQNFAMIEAKIALSMILQ 511

Query: 493 RFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           RFSFELSPSYTHAP +NVT+QPQHGAHLILR L
Sbjct: 512 RFSFELSPSYTHAPITNVTIQPQHGAHLILRML 538

BLAST of Tan0013285 vs. ExPASy TrEMBL
Match: A0A6J1FTW8 (cytochrome P450 CYP72A219-like OS=Cucurbita moschata OX=3662 GN=LOC111446798 PE=3 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 2.0e-257
Identity = 439/525 (83.62%), Postives = 470/525 (89.52%), Query Frame = 0

Query: 1   MTIAAAISISILVGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMAT 60
           MT+  AI+I I+ GSLL WGW FLNWIWLKPKKLEK LR+QGLAG+ YRLLFGDSKDMAT
Sbjct: 3   MTMGGAITIIIVAGSLLVWGWSFLNWIWLKPKKLEKQLREQGLAGNPYRLLFGDSKDMAT 62

Query: 61  AIREARSQPINFSHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLF 120
           AIREARSQ +NFSH IAHRA+ SSH TINKYGKNCF WVGPTPRVYIT PEQLKTVFSL 
Sbjct: 63  AIREARSQSMNFSHHIAHRALPSSHLTINKYGKNCFAWVGPTPRVYITHPEQLKTVFSLI 122

Query: 121 NDIRKAPLFPIARRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIK 180
           NDI KAPLFP AR  GSGGLVSLEG KW+KHRKIINPAFH++KLKDMFPAF ESCNEMIK
Sbjct: 123 NDIPKAPLFPPARTPGSGGLVSLEGSKWAKHRKIINPAFHTEKLKDMFPAFFESCNEMIK 182

Query: 181 KWESMVCESEELGCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTR 240
           KWE MV E EE G FELDVWPDLQNMTAD ISRTAFGSSY+EGKKIFQFLK WVSLLV R
Sbjct: 183 KWEVMVSEEEE-GRFELDVWPDLQNMTADSISRTAFGSSYEEGKKIFQFLKTWVSLLVAR 242

Query: 241 LTKGVYNIPGWRFVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILL 300
           LTKGVYNIPGWRFVPT++NRKMEEV+REI++MVWGIISKRQTAMEA + SNNADLLGILL
Sbjct: 243 LTKGVYNIPGWRFVPTQMNRKMEEVNREIKSMVWGIISKRQTAMEAAQVSNNADLLGILL 302

Query: 301 ESNLNQVQEEQENKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRA 360
           E N          K+ VGMSMEDIISECKLFYFAGQETTA+LLVWTMVLL RYSEWQD+A
Sbjct: 303 EEN---------KKDAVGMSMEDIISECKLFYFAGQETTASLLVWTMVLLGRYSEWQDQA 362

Query: 361 RAEVLEVFGDDNKLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAE 420
           RAEVL+VFG+D K+DFDGL+RLKVV+MIL EVLRLYPP+GILAR I KETKLGNLLLP  
Sbjct: 363 RAEVLDVFGEDKKVDFDGLNRLKVVSMILYEVLRLYPPVGILARGIHKETKLGNLLLPGG 422

Query: 421 VSIGIPIIHIHHDRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAM 480
           VSIGIPIIHIHHD QIWGEDA EFKPERFSEGISKAT N+VCF+PFGWGPRICVGQNFAM
Sbjct: 423 VSIGIPIIHIHHDHQIWGEDADEFKPERFSEGISKATNNKVCFLPFGWGPRICVGQNFAM 482

Query: 481 IEAKIALSMILQRFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           IEAKIAL+MILQ FSF+LSPSYTHAP SNVTVQPQHGAHLILR+L
Sbjct: 483 IEAKIALTMILQHFSFQLSPSYTHAPASNVTVQPQHGAHLILRKL 517

BLAST of Tan0013285 vs. ExPASy TrEMBL
Match: A0A6J1KZI7 (cytochrome P450 CYP72A219-like OS=Cucurbita maxima OX=3661 GN=LOC111499007 PE=3 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 4.7e-230
Identity = 389/513 (75.83%), Postives = 444/513 (86.55%), Query Frame = 0

Query: 13  VGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATAIREARSQPINF 72
           +G L  WGW+ LNW+W+ PK+LEK LRQQGLAG+SYRLLFGDSKD A A+R+AR+QPI+F
Sbjct: 10  LGLLGVWGWKILNWMWVTPKRLEKCLRQQGLAGNSYRLLFGDSKDTAAAVRQARTQPISF 69

Query: 73  SHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFNDIRKAPLFPIA 132
           SH IA RA  SSH TI++YGK+ F W+GP+PRVYIT PEQ+K VFS  NDIRKA  FP  
Sbjct: 70  SHRIAARATPSSHITIDQYGKDSFTWIGPSPRVYITQPEQVKMVFSQINDIRKATSFPFR 129

Query: 133 RRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKKWESMVCESEEL 192
           RR+G GG+VSLEG KW+KHRKIINPAFH +KLKDMFPAF +SC+EMI KWE M+ +    
Sbjct: 130 RRIG-GGVVSLEGAKWAKHRKIINPAFHMEKLKDMFPAFSKSCSEMINKWEMMITKE--- 189

Query: 193 GCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRLTKGVYNIPGWR 252
           GC ELDVWPDLQNM ADVISRTAFGSSY+EGKKIFQ LK W SLL+  + KGVY +PG R
Sbjct: 190 GCCELDVWPDLQNMAADVISRTAFGSSYEEGKKIFQLLKQWASLLMAYVMKGVYFLPGLR 249

Query: 253 FVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLESNLNQVQEEQE 312
           F+PTKLN++M+E++ +I+ M+WGIISKRQ AM+ GEASN+ DLLGILLESN  ++Q E +
Sbjct: 250 FLPTKLNKRMDEINGKIRDMIWGIISKRQNAMKKGEASND-DLLGILLESNSREIQ-EHK 309

Query: 313 NKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRARAEVLEVFGDDN 372
           NK DVGMS+E++I+EC+LFYFAGQETTAALL WTMVLL RYSEWQDRARAEVLEVFGD+ 
Sbjct: 310 NKKDVGMSIEEVIAECRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEVFGDNK 369

Query: 373 KLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEVSIGIPIIHIHH 432
           KLDFDGLSRLKVVTMILNEVLRLYPP+G+LAR I KETKLGNL LP  VSIGIPI+ IH 
Sbjct: 370 KLDFDGLSRLKVVTMILNEVLRLYPPVGMLAREIHKETKLGNLTLPVGVSIGIPIVCIHQ 429

Query: 433 DRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMIEAKIALSMILQ 492
           D  IWGEDA+EFKPERF+EGISKATKNQVCFIPFGWGPRIC+GQNFAMIEAKIALSMILQ
Sbjct: 430 DPTIWGEDASEFKPERFAEGISKATKNQVCFIPFGWGPRICLGQNFAMIEAKIALSMILQ 489

Query: 493 RFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           RFSFELSPSYTHAP SNVT+QPQHGAHLIL  L
Sbjct: 490 RFSFELSPSYTHAPISNVTIQPQHGAHLILHML 516

BLAST of Tan0013285 vs. ExPASy TrEMBL
Match: A0A6J1EFS6 (cytochrome P450 CYP72A219-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432119 PE=3 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 8.0e-230
Identity = 386/513 (75.24%), Postives = 442/513 (86.16%), Query Frame = 0

Query: 13  VGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATAIREARSQPINF 72
           +G L  WGW+ LNW+WL PK+LEK LR QGLAG+SYRLLFGDSKD A A+R+A++QPI+F
Sbjct: 32  LGLLGVWGWKILNWMWLTPKRLEKCLRHQGLAGNSYRLLFGDSKDTAAAVRQAKTQPISF 91

Query: 73  SHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFNDIRKAPLFPIA 132
           SH IA RA  SSH TI++YGK+ F W+GPTPRVYIT PEQ+K VFS  NDIRKA LFP  
Sbjct: 92  SHRIAARATPSSHITIHRYGKDSFTWIGPTPRVYITHPEQVKVVFSQINDIRKATLFPFR 151

Query: 133 RRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKKWESMVCESEEL 192
           RR+G GG+VSLEG KW+KHRKIINPAFH +KLKDMFPAF +SC+EMI KWE M+ +    
Sbjct: 152 RRMG-GGVVSLEGAKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMINKWEMMITKE--- 211

Query: 193 GCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRLTKGVYNIPGWR 252
           GC ELDVWPDLQNM ADVISRTAFGSSY+EGKKIFQ LK W SLL+  + KGVY IPG R
Sbjct: 212 GCCELDVWPDLQNMAADVISRTAFGSSYEEGKKIFQLLKQWASLLMAYVMKGVYFIPGLR 271

Query: 253 FVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLESNLNQVQEEQE 312
           F+PTKLN++M+E++  I+ M+WGII+KRQ AM+ GEASN+ DLLGILLESN  ++Q E +
Sbjct: 272 FLPTKLNKRMDEINGNIRDMIWGIINKRQNAMKKGEASND-DLLGILLESNSREIQ-EHK 331

Query: 313 NKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRARAEVLEVFGDDN 372
           NK DVGMSME++I+EC+LFYFAGQETTAALL WTMVLL RYSEWQDRARAEVLE+FGD+ 
Sbjct: 332 NKKDVGMSMEEVIAECRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEIFGDNK 391

Query: 373 KLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEVSIGIPIIHIHH 432
           K DFDGLSRLK+VTMILNEVLRLYPP+G+L+R I KETKLGNL LP  VSIGIPI+ IH 
Sbjct: 392 KFDFDGLSRLKIVTMILNEVLRLYPPVGLLSREIHKETKLGNLTLPVGVSIGIPIVCIHQ 451

Query: 433 DRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMIEAKIALSMILQ 492
           D  +WGEDA+EFKPERF EGISKATKNQVCFIPFGWGPRIC+GQNFAMIEAKIALSMILQ
Sbjct: 452 DPTLWGEDASEFKPERFVEGISKATKNQVCFIPFGWGPRICLGQNFAMIEAKIALSMILQ 511

Query: 493 RFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           RFSFELSPSYTHAP +NVT+QPQHGAHLILR L
Sbjct: 512 RFSFELSPSYTHAPITNVTIQPQHGAHLILRML 538

BLAST of Tan0013285 vs. ExPASy TrEMBL
Match: A0A6J1E9Z3 (cytochrome P450 CYP72A219-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111432119 PE=3 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 5.2e-229
Identity = 385/513 (75.05%), Postives = 441/513 (85.96%), Query Frame = 0

Query: 13  VGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATAIREARSQPINF 72
           +G L  WGW+ LNW+WL PK+LEK LR QGLAG+SYRLLFGDSKD A A+R+A++QPI+F
Sbjct: 32  LGLLGVWGWKILNWMWLTPKRLEKCLRHQGLAGNSYRLLFGDSKDTAAAVRQAKTQPISF 91

Query: 73  SHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFNDIRKAPLFPIA 132
           SH IA RA  SSH TI++YGK+ F W+GPTPRVYIT PEQ+K VFS  NDIRKA LFP  
Sbjct: 92  SHRIAARATPSSHITIHRYGKDSFTWIGPTPRVYITHPEQVKVVFSQINDIRKATLFPFR 151

Query: 133 RRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKKWESMVCESEEL 192
           RR+G GG+VSLEG KW+KHRKIINPAFH +KLKDMFPAF +SC+EMI KWE M+ +    
Sbjct: 152 RRMG-GGVVSLEGAKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMINKWEMMITKE--- 211

Query: 193 GCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRLTKGVYNIPGWR 252
           GC ELDVWPDLQNM ADVISRTAFGSSY+EGKKIFQ LK W SLL+  + KGVY IPG R
Sbjct: 212 GCCELDVWPDLQNMAADVISRTAFGSSYEEGKKIFQLLKQWASLLMAYVMKGVYFIPGLR 271

Query: 253 FVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLESNLNQVQEEQE 312
           F+PTKLN++M+E++  I+ M+WGII+KRQ AM+ GEASN+ DLLGILLESN  ++Q E +
Sbjct: 272 FLPTKLNKRMDEINGNIRDMIWGIINKRQNAMKKGEASND-DLLGILLESNSREIQ-EHK 331

Query: 313 NKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRARAEVLEVFGDDN 372
           NK DVGMSME++I+EC+LFYFAGQETTAALL WTMVLL RYSEWQDRARAEVLE+FGD+ 
Sbjct: 332 NKKDVGMSMEEVIAECRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEIFGDNK 391

Query: 373 KLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEVSIGIPIIHIHH 432
           K DFDGLSRLK+VTMILNEVLRLYPP+G+L+R I KETKLGNL LP  VSIGIPI+ IH 
Sbjct: 392 KFDFDGLSRLKIVTMILNEVLRLYPPVGLLSREIHKETKLGNLTLPVGVSIGIPIVCIHQ 451

Query: 433 DRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMIEAKIALSMILQ 492
           D  +WGEDA+EFKPERF EGISKATKNQVCFIPFGWGPRIC+GQNFAMIEAKIALSMILQ
Sbjct: 452 DPTLWGEDASEFKPERFVEGISKATKNQVCFIPFGWGPRICLGQNFAMIEAKIALSMILQ 511

Query: 493 RFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           RFSFELSPSYTHAP +NVT+QPQHGAHLIL  L
Sbjct: 512 RFSFELSPSYTHAPITNVTIQPQHGAHLILPML 538

BLAST of Tan0013285 vs. ExPASy TrEMBL
Match: A0A6J1E0T8 (11-oxo-beta-amyrin 30-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111024896 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 7.0e-226
Identity = 392/522 (75.10%), Postives = 447/522 (85.63%), Query Frame = 0

Query: 9   ISILVGSLLA--WGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATAIREAR 68
           +++ VG  LA  WGWR +NWIW++PK+LEK L QQGLAG+SYRLLFGDS D+A A+R AR
Sbjct: 7   LTLGVGLCLAVVWGWRVVNWIWVRPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRAR 66

Query: 69  S-QPINFSHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFNDIRK 128
           S  P++FSHDIA RA  S+H TI+KYGK+CF W G TPRVYI DPEQLK VFS  NDIRK
Sbjct: 67  SGPPMSFSHDIAPRATPSAHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRK 126

Query: 129 APLFP--IARRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKKWE 188
           +  FP  I RR+GS GLV+LEGPKW KHRKIINPAFH +KLKDMFPAF +SC+EMI KWE
Sbjct: 127 SSSFPANIVRRMGS-GLVTLEGPKWXKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWE 186

Query: 189 SMVCESEELGCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRLTK 248
           +MV +    G  ELDVWPDLQNMTADVISRTAFGSSY+EGKKIFQ LK W  LL+TRLT 
Sbjct: 187 TMVLKD---GSCELDVWPDLQNMTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTN 246

Query: 249 GVYNIPGWRFVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLESN 308
            VY IPGW F+PTKLN++ EE+D++I+ MV GIISKRQ+AM+A EASN+ DLLGILLESN
Sbjct: 247 RVYYIPGWSFLPTKLNKRTEEIDKKIRDMVLGIISKRQSAMKADEASND-DLLGILLESN 306

Query: 309 LNQVQEEQENKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRARAE 368
            +Q+Q E  N+ DVGMS++D+ISEC+LFYFAGQETTAALLVWTMVLL  +SEWQDRARAE
Sbjct: 307 ASQIQ-ENGNRKDVGMSIQDVISECRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAE 366

Query: 369 VLEVFGDDNKLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEVSI 428
           VLEVF D+NKLDFDGLSRLK+VTMILNEVLRLYPP+G+LAR I  ETKLGNL LP   SI
Sbjct: 367 VLEVFSDNNKLDFDGLSRLKIVTMILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASI 426

Query: 429 GIPIIHIHHDRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMIEA 488
           GIPI++IH D +IWGEDA EFKPERFSEGISKATKNQVCFIPFGWGPRIC+GQNFAMIEA
Sbjct: 427 GIPIVYIHQDPKIWGEDAGEFKPERFSEGISKATKNQVCFIPFGWGPRICLGQNFAMIEA 486

Query: 489 KIALSMILQRFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           KIAL MIL+RFSFELSPSYTHAP SN+T+QPQHGAHLIL +L
Sbjct: 487 KIALXMILRRFSFELSPSYTHAPVSNITIQPQHGAHLILHRL 522

BLAST of Tan0013285 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 588.2 bits (1515), Expect = 6.6e-168
Identity = 287/525 (54.67%), Postives = 382/525 (72.76%), Query Frame = 0

Query: 1   MTIAAAISISILVGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMAT 60
           M+ +   ++ +LV  ++ W WR + W+W+KPK LE  L++QGL G+ Y  L GD K    
Sbjct: 1   MSFSVVAALPVLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVD 60

Query: 61  AIREARSQPINFSHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLF 120
            + EARS+PIN + DI  R +  +   +N +GK  F+W+GP P + IT+PEQ+K VF+  
Sbjct: 61  MMMEARSKPINVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNKV 120

Query: 121 NDIRKAPLFPIARRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIK 180
           ND  KA  FP+ R L +GGL S +G KW+ HR+IINPAFH +K+K+M PAF   C+E++ 
Sbjct: 121 NDFEKASTFPLIRLL-AGGLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVVC 180

Query: 181 KWESMVCESEELGCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTR 240
           +WE +  + E     E+DVWP L NMTADVIS TAFGSSY EG++IFQ L+  ++ L+ +
Sbjct: 181 QWEKLFTDKE--SPLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQ-LQGELAELIAQ 240

Query: 241 LTKGVYNIPGWRFVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILL 300
             K  Y IPG RF PTK NR+M+ +DRE+  ++ GI+SKR+ A EAGE +N+ DLLGILL
Sbjct: 241 AFKKSY-IPGSRFYPTKSNRRMKAIDREVDVILRGIVSKREKAREAGEPAND-DLLGILL 300

Query: 301 ESNLNQVQEEQENKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRA 360
           ESN    +E Q N    GMS+ED++ ECKLFYFAGQETT+ LLVWTMVLLS + +WQ RA
Sbjct: 301 ESN---SEESQGN----GMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARA 360

Query: 361 RAEVLEVFGDDNKLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAE 420
           R EV++V G++NK D + L+ LKV+TMI NEVLRLYPP+  L R ++KE KLG L LPA 
Sbjct: 361 REEVMQVLGENNKPDMESLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAG 420

Query: 421 VSIGIPIIHIHHDRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAM 480
           + I +P I +  D ++WG+DA +FKPERF +G+SKATKNQV F PFGWGPRIC+GQNFAM
Sbjct: 421 IQIYLPTILVQRDTELWGDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAM 480

Query: 481 IEAKIALSMILQRFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           +EAK+A+++ILQ+FSFELSPSY HAP + +T +PQ GAHLIL +L
Sbjct: 481 LEAKMAMALILQKFSFELSPSYVHAPQTVMTTRPQFGAHLILHKL 512

BLAST of Tan0013285 vs. TAIR 10
Match: AT3G14640.1 (cytochrome P450, family 72, subfamily A, polypeptide 10 )

HSP 1 Score: 562.8 bits (1449), Expect = 3.0e-160
Identity = 281/521 (53.93%), Postives = 369/521 (70.83%), Query Frame = 0

Query: 5   AAISISILVGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATAIRE 64
           A +++S++V  +  W WR L W+W KPK LE  LR+QGLAG+ Y  L GD K     + E
Sbjct: 6   ACVTVSVVVVVVSWWVWRTLQWVWFKPKMLESYLRRQGLAGTPYTPLIGDLKRNVNMLTE 65

Query: 65  ARSQPINFSHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFNDIR 124
           A S+PI  + DI  R +      +  +G+  F W+GP P + I DPE +K VF+   D  
Sbjct: 66  ATSKPIKLTEDITPRVLPHPFQMLKTHGRTFFTWLGPKPTITIMDPELIKEVFNKVYDYP 125

Query: 125 KAPLFPIARRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKKWES 184
           KA  F + R + + G+++ +G KW+KHR+IINPAFH +K+K+M PAF +SC++++ +W  
Sbjct: 126 KAQTFLLGRLIAT-GIINYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCSDVVGEWSK 185

Query: 185 MVCESEELGCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRLTKG 244
           +V +     C E+DVWP L +MT DVISRTAFGSSY EG++IF+     V L++    K 
Sbjct: 186 LVSDKGSSSC-EVDVWPWLVSMTGDVISRTAFGSSYKEGQRIFELQAELVHLILQAFWK- 245

Query: 245 VYNIPGWRFVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLESNL 304
           VY IPG+R++PTK NR+M+   REIQ ++ GI++KR  A EAG+A+ N DLLGILLESNL
Sbjct: 246 VY-IPGYRYLPTKSNRRMKAAAREIQVILKGIVNKRLRAREAGKAAPNDDLLGILLESNL 305

Query: 305 NQVQEEQENKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRARAEV 364
            Q +         GMS ED++ ECKLFYFAGQETT+ LLVW MVLLS + +WQ RAR EV
Sbjct: 306 GQAKGN-------GMSTEDVMEECKLFYFAGQETTSVLLVWAMVLLSHHQDWQARAREEV 365

Query: 365 LEVFGDDNKLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEVSIG 424
            +VFG D + D + LS+LKV+TMIL EVLRLYPP+  L RAIDKE KLG+L LPA V I 
Sbjct: 366 KQVFG-DKEPDTECLSQLKVMTMILYEVLRLYPPVTHLTRAIDKEMKLGDLTLPAGVHIS 425

Query: 425 IPIIHIHHDRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMIEAK 484
           +PI+ +  D  +WG DA EFKPERF +G+SKATK+QV F PF WGPRIC+GQNFAM+EAK
Sbjct: 426 LPIMLVQRDPMLWGTDAAEFKPERFKDGLSKATKSQVSFFPFAWGPRICIGQNFAMLEAK 485

Query: 485 IALSMILQRFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           +A+++ILQ F+FELSPSY HAP + VT+ PQ GAHLILR+L
Sbjct: 486 MAMALILQTFTFELSPSYVHAPQTVVTIHPQFGAHLILRKL 514

BLAST of Tan0013285 vs. TAIR 10
Match: AT3G14690.1 (cytochrome P450, family 72, subfamily A, polypeptide 15 )

HSP 1 Score: 560.5 bits (1443), Expect = 1.5e-159
Identity = 277/521 (53.17%), Postives = 370/521 (71.02%), Query Frame = 0

Query: 5   AAISISILVGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATAIRE 64
           A+++IS+++  +  W WR L W+W KPK LE  LR+QGLAG+ Y  L GD K   T + E
Sbjct: 6   ASVTISVVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSE 65

Query: 65  ARSQPINFSHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFNDIR 124
           ARS+P+  + DI+ R V         YG+  F W GP P + I DPEQ+K VF+   D +
Sbjct: 66  ARSKPLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVFNKVYDFQ 125

Query: 125 KAPLFPIARRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKKWES 184
           K   FP+A  +   GL + +G KW+KHR+IINPAFH +K+K+M PAF +SC E++ +W+ 
Sbjct: 126 KPHTFPLATIIAK-GLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQ 185

Query: 185 MVCESEELGCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRLTKG 244
           +V  S++    E+DVWP L +MTADVISRTAFGSSY EG++IF+       L++    K 
Sbjct: 186 LV--SDKGSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKA 245

Query: 245 VYNIPGWRFVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLESNL 304
              IPG+ ++PTK NR+M+   REIQ ++ GI++KR  A EAGEA ++ DLLGILLESNL
Sbjct: 246 F--IPGYSYLPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGEAPSD-DLLGILLESNL 305

Query: 305 NQVQEEQENKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRARAEV 364
            Q +         GMS ED++ ECKLFYFAGQETT+ LLVWTMVLLS++ +WQ RAR EV
Sbjct: 306 RQTEGN-------GMSTEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEV 365

Query: 365 LEVFGDDNKLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEVSIG 424
            +VFG D + D +GL++LKV+TMIL EVLRLYPP+  L RAI KE KLG+L LP  V I 
Sbjct: 366 KQVFG-DKEPDAEGLNQLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQIS 425

Query: 425 IPIIHIHHDRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMIEAK 484
           +PI+ + HD ++WG DA EF P+RF +G+SKATK+QV F PF WGPRIC+GQNFA++EAK
Sbjct: 426 LPILLVQHDIELWGNDAAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAK 485

Query: 485 IALSMILQRFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           +A+++IL+RFSFE+SPSY HAP + +T+ PQ GA LI+ +L
Sbjct: 486 MAMALILRRFSFEISPSYVHAPYTVITIHPQFGAQLIMHKL 512

BLAST of Tan0013285 vs. TAIR 10
Match: AT3G14660.1 (cytochrome P450, family 72, subfamily A, polypeptide 13 )

HSP 1 Score: 557.4 bits (1435), Expect = 1.2e-158
Identity = 282/521 (54.13%), Postives = 374/521 (71.79%), Query Frame = 0

Query: 5   AAISISILVGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATAIRE 64
           A++++S+ V  +  W WR L  +WLKPK LE  LR+QGLAG+ Y  L GD K   + + E
Sbjct: 6   ASVTVSVAVVVVSWWVWRTLQRVWLKPKMLESYLRRQGLAGTPYTPLVGDLKRNFSMLAE 65

Query: 65  ARSQPINFSHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFNDIR 124
           ARS+PIN + DI  R V      +  +G+  F W GP P + I DPEQ+K VF+   D +
Sbjct: 66  ARSKPINLTDDITPRIVPYPLQMLKTHGRTFFTWFGPIPTITIMDPEQIKEVFNKVYDFQ 125

Query: 125 KAPLFPIARRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKKWES 184
           KA  FP+  RL + GLVS +G KW+KHR+IINPAFH +K+K+M PAF +SC+E++ +W+ 
Sbjct: 126 KAHTFPLG-RLIAAGLVSYDGDKWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIVGEWDK 185

Query: 185 MVCESEELGCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRLTKG 244
           +V + +   C E+D+WP L +MTADVISRTAFGSSY EG++IF+       L++    K 
Sbjct: 186 LVTDKQS-SC-EVDIWPWLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKA 245

Query: 245 VYNIPGWRFVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLESNL 304
           +  IPG+R+ PTK NR+M+   REI+ ++ GI++KR  A EAGEA ++ DLLGILLESNL
Sbjct: 246 I--IPGYRYFPTKGNRRMKAAAREIKFILRGIVNKRLRAREAGEAPSD-DLLGILLESNL 305

Query: 305 NQVQEEQENKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRARAEV 364
            Q +         GMS E+++ ECKLFYFAGQETT  LLVWTMVLLS++ +WQ RAR EV
Sbjct: 306 GQTKGN-------GMSTEELMEECKLFYFAGQETTTVLLVWTMVLLSQHQDWQARAREEV 365

Query: 365 LEVFGDDNKLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEVSIG 424
            +VFG D + D +GL++LKV+TMIL EVLRLYPP+  L RAI KE +LG+L LP  V I 
Sbjct: 366 KQVFG-DKEPDAEGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQIS 425

Query: 425 IPIIHIHHDRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMIEAK 484
           +PI+ I  DR++WG DA EFKP+RF +G+SKATKNQV F PF WGPRIC+GQNFA++EAK
Sbjct: 426 LPILLIQRDRELWGNDAGEFKPDRFKDGLSKATKNQVSFFPFAWGPRICIGQNFALLEAK 485

Query: 485 IALSMILQRFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           +A+++IL++FSFELSPSY HAP + +T  PQ GA LIL +L
Sbjct: 486 MAMTLILRKFSFELSPSYVHAPYTVLTTHPQFGAPLILHKL 512

BLAST of Tan0013285 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 553.9 bits (1426), Expect = 1.4e-157
Identity = 278/521 (53.36%), Postives = 371/521 (71.21%), Query Frame = 0

Query: 5   AAISISILVGSLLAWGWRFLNWIWLKPKKLEKLLRQQGLAGSSYRLLFGDSKDMATAIRE 64
           ++++ S+ V  +  W WR L W+W  PK LE+ LR+QGL+G+SY  L GD K M +   E
Sbjct: 6   SSVTFSLAVVVVSWWVWRTLKWVWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIE 65

Query: 65  ARSQPINFSHDIAHRAVASSHFTINKYGKNCFVWVGPTPRVYITDPEQLKTVFSLFNDIR 124
           A S+PI  + DI  R +      +  +G+    W GP P + I DPEQ+K VF+   D +
Sbjct: 66  ATSKPIKPTDDITPRVMPHPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDFQ 125

Query: 125 KAPLFPIARRLGSGGLVSLEGPKWSKHRKIINPAFHSQKLKDMFPAFLESCNEMIKKWES 184
           KA  FP+++ LG+ GLVS +G KW++HR+IINPAFH +K+K+M   F ESC+E++ +W+ 
Sbjct: 126 KAHTFPLSKILGT-GLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDK 185

Query: 185 MVCESEELGCFELDVWPDLQNMTADVISRTAFGSSYDEGKKIFQFLKNWVSLLVTRLTKG 244
           +V  S++    E+DVWP L +MTADVISRTAFGSSY EG +IF+       L++    K 
Sbjct: 186 LV--SDKGSSCEVDVWPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQK- 245

Query: 245 VYNIPGWRFVPTKLNRKMEEVDREIQTMVWGIISKRQTAMEAGEASNNADLLGILLESNL 304
            + IPG+ ++PTK NR+M+   REIQ ++ GII+KR+ A E+GEA +  DLLGILLESNL
Sbjct: 246 -FFIPGYIYLPTKGNRRMKTAAREIQDILRGIINKRERARESGEAPSE-DLLGILLESNL 305

Query: 305 NQVQEEQENKNDVGMSMEDIISECKLFYFAGQETTAALLVWTMVLLSRYSEWQDRARAEV 364
            Q +         GMS ED++ ECKLFY AGQETT+ LLVWTMVLLS++ +WQ RAR EV
Sbjct: 306 GQTEGN-------GMSTEDMMEECKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEV 365

Query: 365 LEVFGDDNKLDFDGLSRLKVVTMILNEVLRLYPPIGILARAIDKETKLGNLLLPAEVSIG 424
            +VFG D + D +GL++LKV+TMIL EVLRLYPP+  L RAI KE KLG+L LP  V I 
Sbjct: 366 KQVFG-DKQPDTEGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQIS 425

Query: 425 IPIIHIHHDRQIWGEDATEFKPERFSEGISKATKNQVCFIPFGWGPRICVGQNFAMIEAK 484
           +P++ +H D ++WG DA EFKPERF +G+SKATKNQV F PF WGPRIC+GQNF ++EAK
Sbjct: 426 LPVLLVHRDTELWGNDAGEFKPERFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAK 485

Query: 485 IALSMILQRFSFELSPSYTHAPTSNVTVQPQHGAHLILRQL 526
           +A+S+ILQRFSFELSPSY HAP + +T+ PQ GAHL+L +L
Sbjct: 486 MAMSLILQRFSFELSPSYVHAPYTIITLYPQFGAHLMLHKL 512