Tan0013209 (gene) Snake gourd v1

BLAST of Tan0013209 vs. ExPASy Swiss-Prot
Match: Q9ZT63 (Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2)

HSP 1 Score: 541.2 bits (1393), Expect = 1.0e-152
Identity = 299/418 (71.53%), Postives = 335/418 (80.14%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDAKNSSKDAKERSASMRKLF 60
           ME  +  H HI+EV          IV SK+CGEAP CGFSD+KN+S DA ERSASMRKL 
Sbjct: 1   MESSSPHHSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLC 60

Query: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAVVLC+VFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATPRQ+YG
Sbjct: 61  IAVVLCLVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEHHGISVTTHHHHLHHRKEEEVTTSDGVK 240
           LGH+HGH+H HGH HGH  H+H           HG++VTTHHHH  H    E   S G  
Sbjct: 181 LGHDHGHSHGHGHGHGHDHHNHS----------HGVTVTTHHHHHDH----EHGHSHGHG 240

Query: 241 DDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQRNINVQGAYLHVLGDSIQSIGVMIGG 300
           +D HHAH       + E   D    +V   +K++RNIN+QGAYLHVLGDSIQS+GVMIGG
Sbjct: 241 EDKHHAH-----GDVTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDSIQSVGVMIGG 300

Query: 301 AAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEMD 360
           A IWYN E+KI+DLICTL FS IVL TTI M+RNILEVLMESTPREIDATKLEKGL EM+
Sbjct: 301 AIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDATKLEKGLLEME 360

Query: 361 EVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQIER 419
           EVVA+HELHIWAITVGKVLLACHV I+PEA+ADMVL+KVI+YIRREYNISHVTIQIER
Sbjct: 361 EVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNISHVTIQIER 398

BLAST of Tan0013209 vs. ExPASy Swiss-Prot
Match: Q9LXS1 (Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2)

HSP 1 Score: 521.5 bits (1342), Expect = 8.5e-147
Identity = 292/420 (69.52%), Postives = 331/420 (78.81%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVG-SKICGEAPSCGFSDAKNSSKDAKERSASMRKL 60
           M+ H H+H H++++CG+V +   S+VG  K CGEAP CGFSDAK SS +A+ER+ASMRKL
Sbjct: 16  MKDHIHEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFSDAKTSSIEAQERAASMRKL 75

Query: 61  FIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY 120
            IAV+LC +F+ VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGW+A P+QSY
Sbjct: 76  LIAVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQSY 135

Query: 121 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL 180
           GFFRIEILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G LMFAVSA GL+VNIAMA+
Sbjct: 136 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMAI 195

Query: 181 LLGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEHHGISVTTHHHHLHHRKEEEVTTSDGV 240
           LLGH+HGH H H H +GHG H H HG        HGI+ T HHH   H   +E   SD  
Sbjct: 196 LLGHDHGHGHGHSHDNGHG-HSHDHG--------HGIAATEHHHDSGH---DESQLSD-- 255

Query: 241 KDDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQRNINVQGAYLHVLGDSIQSIGVMIG 300
                                      V   QKKQRN+N+QGAYLHVLGDSIQS+GVMIG
Sbjct: 256 ---------------------------VLIEQKKQRNVNIQGAYLHVLGDSIQSVGVMIG 315

Query: 301 GAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEM 360
           GA IWY  E+KILDLICTL+FS IVL TTI MLRNILEVLMESTPREID T LEKG+CE+
Sbjct: 316 GAIIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEI 375

Query: 361 DEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQIERQ 420
           +EVVA+HELHIWAITVGK+LLACHV I+PEA ADMVLDK+I+YI+RE+NISHVTIQIERQ
Sbjct: 376 EEVVAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIERQ 393

BLAST of Tan0013209 vs. ExPASy Swiss-Prot
Match: Q688R1 (Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 5.7e-143
Identity = 294/428 (68.69%), Postives = 330/428 (77.10%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDAKNSSKDAKERSASMRKLF 60
           M+ HN     I EV  D+ +      G+K+C  A +C FSD+ NSSKDA+ER ASMRKL 
Sbjct: 1   MDSHNSAPPQIAEVRMDISSSTSVAAGNKVCRGA-ACDFSDSSNSSKDARERMASMRKLI 60

Query: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAV+LCI+FM+VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATP+QSYG
Sbjct: 61  IAVILCIIFMAVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPQQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQ+IWLLAGILVYEAIVRLIN  GEV+G LMFAVSAFGL VNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLAGILVYEAIVRLINESGEVQGSLMFAVSAFGLFVNIIMAVL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEHHGISVTTHHHHLHHRKE---EEVTTS- 240
           LGH+HGH H HGH HGH  HDH HG  +H H HH       H H+HH ++     +T + 
Sbjct: 181 LGHDHGHGHGHGHGHGH-SHDHDHGGSDHDHHHH---EDQEHGHVHHHEDGHGNSITVNL 240

Query: 241 ---DGVKDDHHHAHEVSVTAPLLES--CCDDENQKVTKAQKKQRNINVQGAYLHVLGDSI 300
               G    HH A E     PLL+S   CD        A+K +RNINV  AYLHVLGDSI
Sbjct: 241 HHHPGTGHHHHDAEE-----PLLKSDAGCDSTQSGAKDAKKARRNINVHSAYLHVLGDSI 300

Query: 301 QSIGVMIGGAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATK 360
           QSIGVMIGGA IWY  E+KI+DLICTLIFS IVL TTI+MLRNILEVLMESTPREIDAT 
Sbjct: 301 QSIGVMIGGAIIWYKPEWKIIDLICTLIFSVIVLFTTIKMLRNILEVLMESTPREIDATS 360

Query: 361 LEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISH 420
           LE GL +MD VVA+HELHIWAITVGKVLLACHV I  +A+AD +LDKVI YI+ EYNISH
Sbjct: 361 LENGLRDMDGVVAVHELHIWAITVGKVLLACHVTITQDADADQMLDKVIGYIKSEYNISH 418

BLAST of Tan0013209 vs. ExPASy Swiss-Prot
Match: Q9M271 (Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 1.8e-112
Identity = 239/392 (60.97%), Postives = 274/392 (69.90%), Query Frame = 0

Query: 28  SKICGEAPSCGFSDAKNSSKDAKERSASMRKLFIAVVLCIVFMSVEVVGGIKANSLAILT 87
           SK+CGE  +CGFS    SS DAK+R+ASMRKL   VVLC++FMS+EVV GIKANSLAIL 
Sbjct: 6   SKVCGET-ACGFS---TSSSDAKKRAASMRKLCFVVVLCLLFMSIEVVCGIKANSLAILA 65

Query: 88  DAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEA 147
           DAAHLL+DV AFAIS+ SLWAS WEA PRQSYGFFRIEILG LVSIQ+IWLL GILVYEA
Sbjct: 66  DAAHLLTDVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEA 125

Query: 148 IVRLINGPG-EVKGLLMFAVSAFGLVVNIAMALLLGHEHGHAHSHGHSHGHGDHDHGHGS 207
           + RL+     +V G  M  V+AFGLVVNI M ++LGH+HGH H HGHS     HDHGH  
Sbjct: 126 VTRLVQETNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHDHGHS-----HDHGHSY 185

Query: 208 HEHGHEHHGISVTTHHHHLHHRKEEEVTTSDGVKDDHHHAHEVSVTAPLLESCCDDENQK 267
            E   +                                          LLE         
Sbjct: 186 GERAEQ------------------------------------------LLEK-------- 245

Query: 268 VTKAQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAAIWYNSEYKILDLICTLIFSAIVLC 327
                K+ RNINVQGAYLHVLGD IQSIGVMIGG  IWYN ++K++DLICTL FS IVL 
Sbjct: 246 ----SKEIRNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNPKWKVIDLICTLFFSVIVLG 305

Query: 328 TTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVII 387
           TTI+MLR+ILEVLMESTPREIDA +LEKGL E++EVV +HELHIWAITVGK L +CHV +
Sbjct: 306 TTIKMLRSILEVLMESTPREIDARQLEKGLMEIEEVVDVHELHIWAITVGKALFSCHVKV 334

Query: 388 KPEANADMVLDKVIEYIRREYNISHVTIQIER 419
           +PEA  +MVL+KVI+YI REY ISHVTIQIER
Sbjct: 366 RPEAGDEMVLNKVIDYIWREYRISHVTIQIER 334

BLAST of Tan0013209 vs. ExPASy Swiss-Prot
Match: Q6DBM8 (Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 8.4e-78
Identity = 164/384 (42.71%), Postives = 240/384 (62.50%), Query Frame = 0

Query: 36  SCGFSDAKNSSKDAKERSASMRKLFIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSD 95
           SC F+  ++   + KER  S R+L   + L ++ MSV++VGG KANSLA++TDAAHLLSD
Sbjct: 35  SCAFTRQEHCVSETKEREESTRRLSSLIFLYLIVMSVQIVGGFKANSLAVMTDAAHLLSD 94

Query: 96  VAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGP 155
           VA   +SL ++  S WEA PR S+GF R+E+L A +S+Q+IWL++G++++EAI RL++  
Sbjct: 95  VAGLCVSLLAIKVSSWEANPRNSFGFKRLEVLAAFLSVQLIWLVSGVIIHEAIQRLLSRS 154

Query: 156 GEVKGLLMFAVSAFGLVVNIAMALLLGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEHHG 215
            EV G +MF +SAFG  +N+ M L LG        H HSH H DH H H +H+H H+HH 
Sbjct: 155 REVNGEIMFGISAFGFFMNLVMVLWLG--------HNHSHHHHDHHHHHHNHKHQHQHH- 214

Query: 216 ISVTTHHHHLHHRKEEEVTTSDGVKDDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQR 275
                H   +   +EEE+                    PL          K  K+  K+ 
Sbjct: 215 -----HKEVVAEEEEEEMN-------------------PL----------KGEKSSSKEM 274

Query: 276 NINVQGAYLHVLGDSIQSIGVMIGGAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNI 335
           NIN+QGAYLH + D IQS+GVMIGG  IW   ++ ++DLICTL+FSA  L  T+ +L+NI
Sbjct: 275 NINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDLICTLVFSAFALAATLPILKNI 334

Query: 336 LEVLMESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMV 395
             +LME  PR++D  KLE+GL  +D V  +++LH+W ITVG+++L+CH++ +P A+   +
Sbjct: 335 FGILMERVPRDMDIEKLERGLKRIDGVKIVYDLHVWEITVGRIVLSCHILPEPGASPKEI 375

Query: 396 LDKVIEYIRREYNISHVTIQIERQ 420
           +  V  + R+ Y I H T+Q+E +
Sbjct: 395 ITGVRNFCRKSYGIYHATVQVESE 375

BLAST of Tan0013209 vs. NCBI nr
Match: XP_023534426.1 (metal tolerance protein 1-like [Cucurbita pepo subsp. pepo] >XP_023534427.1 metal tolerance protein 1-like [Cucurbita pepo subsp. pepo] >XP_023534428.1 metal tolerance protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 702.6 bits (1812), Expect = 2.1e-198
Identity = 376/423 (88.89%), Postives = 386/423 (91.25%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDAKNSSKDAKERSASMRKLF 60
           MEV NHDHGH+IEV GDVQAVGPSIVGSKICGEAPSCGF DAKNSS D +ER+ASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHE----HHGISVTTHHHHLHHRKEEEVTTS 240
           LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHE    HHGISVT HHHH H   EEE    
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHPHPHHEEEGAAG 240

Query: 241 DGVKDDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQRNINVQGAYLHVLGDSIQSIGV 300
           D VK  HHHAHE SVT PLLES     +QKVTK QK++RNINVQGAYLHVLGDSIQSIGV
Sbjct: 241 DSVKHHHHHAHEESVTTPLLES----HSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGV 300

Query: 301 MIGGAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGL 360
           MIGGA IWY  EYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLEKGL
Sbjct: 301 MIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGL 360

Query: 361 CEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQI 420
           CEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VIEYI+REYNISHVTIQI
Sbjct: 361 CEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQI 419

BLAST of Tan0013209 vs. NCBI nr
Match: XP_022159643.1 (metal tolerance protein 1-like [Momordica charantia] >XP_022159644.1 metal tolerance protein 1-like [Momordica charantia] >XP_022159645.1 metal tolerance protein 1-like [Momordica charantia])

HSP 1 Score: 698.4 bits (1801), Expect = 3.9e-197
Identity = 384/436 (88.07%), Postives = 394/436 (90.37%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDAKNSSKDAKERSASMRKLF 60
           MEV +HDHGHIIEVCGDVQAVGPSI GSKICG+AP CGFSDAKNSSKDAKERSASMRKLF
Sbjct: 1   MEVQSHDHGHIIEVCGDVQAVGPSITGSKICGDAP-CGFSDAKNSSKDAKERSASMRKLF 60

Query: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAI+RLINGPGEVKGLLMF VSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIIRLINGPGEVKGLLMFVVSAFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHD---------HGHGSHEHGHE------HHGISVTTHHHHL 240
           LGHEHGHAHSHGHSHGHG HD         HGHGSHEHGHE      HHGISVTTHHHH 
Sbjct: 181 LGHEHGHAHSHGHSHGHGGHDHEHGHGSHEHGHGSHEHGHEDHTNRHHHGISVTTHHHH- 240

Query: 241 HHRKEEEVTTSDGVKDDHHHAHEVSVTAPLLESCCDDE--NQKVTKAQKKQRNINVQGAY 300
           HH  EEE  TS+  K DH H HEV  TAPLLES   DE  +QK T+  KKQRNINVQGAY
Sbjct: 241 HH--EEEGATSNAAKHDHVHTHEVDTTAPLLESYYKDESHSQKGTEKPKKQRNINVQGAY 300

Query: 301 LHVLGDSIQSIGVMIGGAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMEST 360
           LHVLGDSIQSIGVMIGGA IWYN +YKILDLICTLIFSAIVLCTTIQMLRNILEVLMEST
Sbjct: 301 LHVLGDSIQSIGVMIGGAIIWYNPKYKILDLICTLIFSAIVLCTTIQMLRNILEVLMEST 360

Query: 361 PREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYI 420
           PREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVI+KPEANADMVLDKVI+YI
Sbjct: 361 PREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIDYI 420

BLAST of Tan0013209 vs. NCBI nr
Match: KAG6605616.1 (Metal tolerance protein A2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035528.1 Metal tolerance protein A2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 695.3 bits (1793), Expect = 3.3e-196
Identity = 377/426 (88.50%), Postives = 387/426 (90.85%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDAKNSSKDAKERSASMRKLF 60
           MEV NHDHGH+IEV GDVQAVGPSIVGSKICGEAPSCGF DAKNSS D +ER+ASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHE----HHGISVTTHHHHLHHRKEEEVTTS 240
           LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHE    HHGISVT HHHH H   EEE    
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHH-HPHHEEEGAAG 240

Query: 241 DGVK---DDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQRNINVQGAYLHVLGDSIQS 300
           DGVK     HHHAHE SVT PLLES     +QKVTK QK++RNINVQGAYLHVLGDSIQS
Sbjct: 241 DGVKHHHHHHHHAHEESVTTPLLES----HSQKVTKTQKRERNINVQGAYLHVLGDSIQS 300

Query: 301 IGVMIGGAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLE 360
           IGVMIGGA IWY  EYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLE
Sbjct: 301 IGVMIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLE 360

Query: 361 KGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVT 420
           KGLCEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VIEYI+REYNISHVT
Sbjct: 361 KGLCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVT 420

BLAST of Tan0013209 vs. NCBI nr
Match: XP_022958195.1 (metal tolerance protein 1-like [Cucurbita moschata] >XP_022958198.1 metal tolerance protein 1-like [Cucurbita moschata] >XP_022958199.1 metal tolerance protein 1-like [Cucurbita moschata] >XP_022958200.1 metal tolerance protein 1-like [Cucurbita moschata] >XP_022958201.1 metal tolerance protein 1-like [Cucurbita moschata])

HSP 1 Score: 693.7 bits (1789), Expect = 9.6e-196
Identity = 375/424 (88.44%), Postives = 386/424 (91.04%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDAKNSSKDAKERSASMRKLF 60
           MEV N DHGH+IEVCGDVQAVGPSIVGSKICGEAPSCGF DAKNSS D +ER+ASMRKLF
Sbjct: 1   MEVQNPDHGHMIEVCGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHE----HHGISVTTHHHHLHHRKEEEVTTS 240
           LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHE    HHGISVT HHHH H   EEE    
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHH-HPHHEEEGAAG 240

Query: 241 DGVK-DDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQRNINVQGAYLHVLGDSIQSIG 300
           DGVK   HHHAHE SVT PLLES     +QKVT  QK++RNINVQGAYLHVLGDSIQSIG
Sbjct: 241 DGVKHHHHHHAHEESVTTPLLES----HSQKVTNTQKRERNINVQGAYLHVLGDSIQSIG 300

Query: 301 VMIGGAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKG 360
           VMIGGA IWY  EYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLEKG
Sbjct: 301 VMIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKG 360

Query: 361 LCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQ 420
           LCEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VIEYI+REYNISH+TIQ
Sbjct: 361 LCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHITIQ 419

BLAST of Tan0013209 vs. NCBI nr
Match: XP_022995643.1 (metal tolerance protein 1-like isoform X2 [Cucurbita maxima] >XP_022995644.1 metal tolerance protein 1-like isoform X2 [Cucurbita maxima] >XP_022995646.1 metal tolerance protein 1-like isoform X2 [Cucurbita maxima] >XP_022995647.1 metal tolerance protein 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 690.6 bits (1781), Expect = 8.1e-195
Identity = 371/423 (87.71%), Postives = 384/423 (90.78%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDAKNSSKDAKERSASMRKLF 60
           MEV NHDHGH+IEV GDVQAVGPSIVGSKICGEAPSCGF DAKNSS D +ER+ASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHE----HHGISVTTHHHHLHHRKEEEVTTS 240
           LGHEHGHAHSHGHSHGHGDH+HG G HEHGHE    HHGISVT HHHH H   EEE    
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHH-HPHHEEEGAAG 240

Query: 241 DGVKDDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQRNINVQGAYLHVLGDSIQSIGV 300
           D VK  HHHAHE SVT PLLES     +QKVTK QK++RNINVQGAYLHVLGDSIQS+GV
Sbjct: 241 DSVKHQHHHAHEESVTTPLLES----HSQKVTKTQKRERNINVQGAYLHVLGDSIQSVGV 300

Query: 301 MIGGAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGL 360
           M+GGA IWY  EYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLEKGL
Sbjct: 301 MVGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGL 360

Query: 361 CEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQI 420
           CEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VIEYI+REYNISHVTIQI
Sbjct: 361 CEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQI 418

BLAST of Tan0013209 vs. ExPASy TrEMBL
Match: A0A6J1DZE1 (metal tolerance protein 1-like OS=Momordica charantia OX=3673 GN=LOC111025992 PE=4 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 1.9e-197
Identity = 384/436 (88.07%), Postives = 394/436 (90.37%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDAKNSSKDAKERSASMRKLF 60
           MEV +HDHGHIIEVCGDVQAVGPSI GSKICG+AP CGFSDAKNSSKDAKERSASMRKLF
Sbjct: 1   MEVQSHDHGHIIEVCGDVQAVGPSITGSKICGDAP-CGFSDAKNSSKDAKERSASMRKLF 60

Query: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAI+RLINGPGEVKGLLMF VSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIIRLINGPGEVKGLLMFVVSAFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHD---------HGHGSHEHGHE------HHGISVTTHHHHL 240
           LGHEHGHAHSHGHSHGHG HD         HGHGSHEHGHE      HHGISVTTHHHH 
Sbjct: 181 LGHEHGHAHSHGHSHGHGGHDHEHGHGSHEHGHGSHEHGHEDHTNRHHHGISVTTHHHH- 240

Query: 241 HHRKEEEVTTSDGVKDDHHHAHEVSVTAPLLESCCDDE--NQKVTKAQKKQRNINVQGAY 300
           HH  EEE  TS+  K DH H HEV  TAPLLES   DE  +QK T+  KKQRNINVQGAY
Sbjct: 241 HH--EEEGATSNAAKHDHVHTHEVDTTAPLLESYYKDESHSQKGTEKPKKQRNINVQGAY 300

Query: 301 LHVLGDSIQSIGVMIGGAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMEST 360
           LHVLGDSIQSIGVMIGGA IWYN +YKILDLICTLIFSAIVLCTTIQMLRNILEVLMEST
Sbjct: 301 LHVLGDSIQSIGVMIGGAIIWYNPKYKILDLICTLIFSAIVLCTTIQMLRNILEVLMEST 360

Query: 361 PREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYI 420
           PREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVI+KPEANADMVLDKVI+YI
Sbjct: 361 PREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIDYI 420

BLAST of Tan0013209 vs. ExPASy TrEMBL
Match: A0A6J1H2S0 (metal tolerance protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459497 PE=4 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 4.6e-196
Identity = 375/424 (88.44%), Postives = 386/424 (91.04%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDAKNSSKDAKERSASMRKLF 60
           MEV N DHGH+IEVCGDVQAVGPSIVGSKICGEAPSCGF DAKNSS D +ER+ASMRKLF
Sbjct: 1   MEVQNPDHGHMIEVCGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHE----HHGISVTTHHHHLHHRKEEEVTTS 240
           LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHE    HHGISVT HHHH H   EEE    
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHH-HPHHEEEGAAG 240

Query: 241 DGVK-DDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQRNINVQGAYLHVLGDSIQSIG 300
           DGVK   HHHAHE SVT PLLES     +QKVT  QK++RNINVQGAYLHVLGDSIQSIG
Sbjct: 241 DGVKHHHHHHAHEESVTTPLLES----HSQKVTNTQKRERNINVQGAYLHVLGDSIQSIG 300

Query: 301 VMIGGAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKG 360
           VMIGGA IWY  EYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLEKG
Sbjct: 301 VMIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKG 360

Query: 361 LCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQ 420
           LCEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VIEYI+REYNISH+TIQ
Sbjct: 361 LCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHITIQ 419

BLAST of Tan0013209 vs. ExPASy TrEMBL
Match: A0A6J1JZI6 (metal tolerance protein 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491116 PE=4 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 3.9e-195
Identity = 371/423 (87.71%), Postives = 384/423 (90.78%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDAKNSSKDAKERSASMRKLF 60
           MEV NHDHGH+IEV GDVQAVGPSIVGSKICGEAPSCGF DAKNSS D +ER+ASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHE----HHGISVTTHHHHLHHRKEEEVTTS 240
           LGHEHGHAHSHGHSHGHGDH+HG G HEHGHE    HHGISVT HHHH H   EEE    
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHH-HPHHEEEGAAG 240

Query: 241 DGVKDDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQRNINVQGAYLHVLGDSIQSIGV 300
           D VK  HHHAHE SVT PLLES     +QKVTK QK++RNINVQGAYLHVLGDSIQS+GV
Sbjct: 241 DSVKHQHHHAHEESVTTPLLES----HSQKVTKTQKRERNINVQGAYLHVLGDSIQSVGV 300

Query: 301 MIGGAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGL 360
           M+GGA IWY  EYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLEKGL
Sbjct: 301 MVGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGL 360

Query: 361 CEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQI 420
           CEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VIEYI+REYNISHVTIQI
Sbjct: 361 CEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQI 418

BLAST of Tan0013209 vs. ExPASy TrEMBL
Match: A0A6J1JZI0 (metal tolerance protein 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491116 PE=4 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 3.9e-195
Identity = 371/423 (87.71%), Postives = 384/423 (90.78%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDAKNSSKDAKERSASMRKLF 60
           MEV NHDHGH+IEV GDVQAVGPSIVGSKICGEAPSCGF DAKNSS D +ER+ASMRKLF
Sbjct: 3   MEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 62

Query: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 63  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 122

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 123 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 182

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHE----HHGISVTTHHHHLHHRKEEEVTTS 240
           LGHEHGHAHSHGHSHGHGDH+HG G HEHGHE    HHGISVT HHHH H   EEE    
Sbjct: 183 LGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHH-HPHHEEEGAAG 242

Query: 241 DGVKDDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQRNINVQGAYLHVLGDSIQSIGV 300
           D VK  HHHAHE SVT PLLES     +QKVTK QK++RNINVQGAYLHVLGDSIQS+GV
Sbjct: 243 DSVKHQHHHAHEESVTTPLLES----HSQKVTKTQKRERNINVQGAYLHVLGDSIQSVGV 302

Query: 301 MIGGAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGL 360
           M+GGA IWY  EYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLEKGL
Sbjct: 303 MVGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGL 362

Query: 361 CEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQI 420
           CEM+EVVA+HELHIWAITVGKVLLACHVIIKPEANADMVLD VIEYI+REYNISHVTIQI
Sbjct: 363 CEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQI 420

BLAST of Tan0013209 vs. ExPASy TrEMBL
Match: A0A0A0KD36 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G080940 PE=4 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 3.3e-194
Identity = 373/426 (87.56%), Postives = 386/426 (90.61%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDAKNSSKDAKERSASMRKLF 60
           +++   DHGHIIEVCGDVQAVGPSIVGSKICG+APSCGFSDAKNSSKDAKERSASMRKL 
Sbjct: 6   LQMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNSSKDAKERSASMRKLC 65

Query: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 66  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 125

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKG LMFAVS FGLVVNIAMALL
Sbjct: 126 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGFLMFAVSTFGLVVNIAMALL 185

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHE----HHGISVTTHHHHLHHRKEEEVTTS 240
           LGHEHGHAHSHGH+HGHG+HDHGHGSHEHG E     HGISVT HHHH     EE+   S
Sbjct: 186 LGHEHGHAHSHGHNHGHGEHDHGHGSHEHGEEDHRHRHGISVTMHHHH----HEEKRAAS 245

Query: 241 DGVK---DDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQRNINVQGAYLHVLGDSIQS 300
           DGV+     HHH H+ S T PLL     D +QKVTKAQKKQRNINVQGAYLHVLGDSIQS
Sbjct: 246 DGVEHHHHHHHHKHKESTTVPLL-----DSSQKVTKAQKKQRNINVQGAYLHVLGDSIQS 305

Query: 301 IGVMIGGAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLE 360
           IGVMIGGA IWY  EY ILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPRE+DATKLE
Sbjct: 306 IGVMIGGAIIWYKPEYMILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREVDATKLE 365

Query: 361 KGLCEMDEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVT 420
           KGLCEM+EVVAIHELHIWAITVGK+LLACHVIIKPEANADMVLDKVIEYIRREYNISHVT
Sbjct: 366 KGLCEMEEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIEYIRREYNISHVT 422

BLAST of Tan0013209 vs. TAIR 10
Match: AT2G46800.1 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 541.2 bits (1393), Expect = 7.4e-154
Identity = 299/418 (71.53%), Postives = 335/418 (80.14%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDAKNSSKDAKERSASMRKLF 60
           ME  +  H HI+EV          IV SK+CGEAP CGFSD+KN+S DA ERSASMRKL 
Sbjct: 1   MESSSPHHSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLC 60

Query: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAVVLC+VFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATPRQ+YG
Sbjct: 61  IAVVLCLVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEHHGISVTTHHHHLHHRKEEEVTTSDGVK 240
           LGH+HGH+H HGH HGH  H+H           HG++VTTHHHH  H    E   S G  
Sbjct: 181 LGHDHGHSHGHGHGHGHDHHNHS----------HGVTVTTHHHHHDH----EHGHSHGHG 240

Query: 241 DDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQRNINVQGAYLHVLGDSIQSIGVMIGG 300
           +D HHAH       + E   D    +V   +K++RNIN+QGAYLHVLGDSIQS+GVMIGG
Sbjct: 241 EDKHHAH-----GDVTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDSIQSVGVMIGG 300

Query: 301 AAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEMD 360
           A IWYN E+KI+DLICTL FS IVL TTI M+RNILEVLMESTPREIDATKLEKGL EM+
Sbjct: 301 AIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDATKLEKGLLEME 360

Query: 361 EVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQIER 419
           EVVA+HELHIWAITVGKVLLACHV I+PEA+ADMVL+KVI+YIRREYNISHVTIQIER
Sbjct: 361 EVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNISHVTIQIER 398

BLAST of Tan0013209 vs. TAIR 10
Match: AT2G46800.2 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 541.2 bits (1393), Expect = 7.4e-154
Identity = 299/418 (71.53%), Postives = 335/418 (80.14%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDAKNSSKDAKERSASMRKLF 60
           ME  +  H HI+EV          IV SK+CGEAP CGFSD+KN+S DA ERSASMRKL 
Sbjct: 1   MESSSPHHSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLC 60

Query: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAVVLC+VFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATPRQ+YG
Sbjct: 61  IAVVLCLVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEHHGISVTTHHHHLHHRKEEEVTTSDGVK 240
           LGH+HGH+H HGH HGH  H+H           HG++VTTHHHH  H    E   S G  
Sbjct: 181 LGHDHGHSHGHGHGHGHDHHNHS----------HGVTVTTHHHHHDH----EHGHSHGHG 240

Query: 241 DDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQRNINVQGAYLHVLGDSIQSIGVMIGG 300
           +D HHAH       + E   D    +V   +K++RNIN+QGAYLHVLGDSIQS+GVMIGG
Sbjct: 241 EDKHHAH-----GDVTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDSIQSVGVMIGG 300

Query: 301 AAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEMD 360
           A IWYN E+KI+DLICTL FS IVL TTI M+RNILEVLMESTPREIDATKLEKGL EM+
Sbjct: 301 AIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDATKLEKGLLEME 360

Query: 361 EVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQIER 419
           EVVA+HELHIWAITVGKVLLACHV I+PEA+ADMVL+KVI+YIRREYNISHVTIQIER
Sbjct: 361 EVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNISHVTIQIER 398

BLAST of Tan0013209 vs. TAIR 10
Match: AT3G58810.1 (metal tolerance protein A2 )

HSP 1 Score: 521.5 bits (1342), Expect = 6.1e-148
Identity = 292/420 (69.52%), Postives = 331/420 (78.81%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVG-SKICGEAPSCGFSDAKNSSKDAKERSASMRKL 60
           M+ H H+H H++++CG+V +   S+VG  K CGEAP CGFSDAK SS +A+ER+ASMRKL
Sbjct: 55  MKDHIHEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFSDAKTSSIEAQERAASMRKL 114

Query: 61  FIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY 120
            IAV+LC +F+ VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGW+A P+QSY
Sbjct: 115 LIAVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQSY 174

Query: 121 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL 180
           GFFRIEILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G LMFAVSA GL+VNIAMA+
Sbjct: 175 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMAI 234

Query: 181 LLGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEHHGISVTTHHHHLHHRKEEEVTTSDGV 240
           LLGH+HGH H H H +GHG H H HG        HGI+ T HHH   H   +E   SD  
Sbjct: 235 LLGHDHGHGHGHSHDNGHG-HSHDHG--------HGIAATEHHHDSGH---DESQLSD-- 294

Query: 241 KDDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQRNINVQGAYLHVLGDSIQSIGVMIG 300
                                      V   QKKQRN+N+QGAYLHVLGDSIQS+GVMIG
Sbjct: 295 ---------------------------VLIEQKKQRNVNIQGAYLHVLGDSIQSVGVMIG 354

Query: 301 GAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEM 360
           GA IWY  E+KILDLICTL+FS IVL TTI MLRNILEVLMESTPREID T LEKG+CE+
Sbjct: 355 GAIIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEI 414

Query: 361 DEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQIERQ 420
           +EVVA+HELHIWAITVGK+LLACHV I+PEA ADMVLDK+I+YI+RE+NISHVTIQIERQ
Sbjct: 415 EEVVAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIERQ 432

BLAST of Tan0013209 vs. TAIR 10
Match: AT3G58810.2 (metal tolerance protein A2 )

HSP 1 Score: 521.5 bits (1342), Expect = 6.1e-148
Identity = 292/420 (69.52%), Postives = 331/420 (78.81%), Query Frame = 0

Query: 1   MEVHNHDHGHIIEVCGDVQAVGPSIVG-SKICGEAPSCGFSDAKNSSKDAKERSASMRKL 60
           M+ H H+H H++++CG+V +   S+VG  K CGEAP CGFSDAK SS +A+ER+ASMRKL
Sbjct: 16  MKDHIHEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFSDAKTSSIEAQERAASMRKL 75

Query: 61  FIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY 120
            IAV+LC +F+ VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGW+A P+QSY
Sbjct: 76  LIAVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQSY 135

Query: 121 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL 180
           GFFRIEILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G LMFAVSA GL+VNIAMA+
Sbjct: 136 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMAI 195

Query: 181 LLGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEHHGISVTTHHHHLHHRKEEEVTTSDGV 240
           LLGH+HGH H H H +GHG H H HG        HGI+ T HHH   H   +E   SD  
Sbjct: 196 LLGHDHGHGHGHSHDNGHG-HSHDHG--------HGIAATEHHHDSGH---DESQLSD-- 255

Query: 241 KDDHHHAHEVSVTAPLLESCCDDENQKVTKAQKKQRNINVQGAYLHVLGDSIQSIGVMIG 300
                                      V   QKKQRN+N+QGAYLHVLGDSIQS+GVMIG
Sbjct: 256 ---------------------------VLIEQKKQRNVNIQGAYLHVLGDSIQSVGVMIG 315

Query: 301 GAAIWYNSEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEM 360
           GA IWY  E+KILDLICTL+FS IVL TTI MLRNILEVLMESTPREID T LEKG+CE+
Sbjct: 316 GAIIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEI 375

Query: 361 DEVVAIHELHIWAITVGKVLLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQIERQ 420
           +EVVA+HELHIWAITVGK+LLACHV I+PEA ADMVLDK+I+YI+RE+NISHVTIQIERQ
Sbjct: 376 EEVVAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIERQ 393

BLAST of Tan0013209 vs. TAIR 10
Match: AT3G61940.1 (Cation efflux family protein )

HSP 1 Score: 407.5 bits (1046), Expect = 1.3e-113
Identity = 239/392 (60.97%), Postives = 274/392 (69.90%), Query Frame = 0

Query: 28  SKICGEAPSCGFSDAKNSSKDAKERSASMRKLFIAVVLCIVFMSVEVVGGIKANSLAILT 87
           SK+CGE  +CGFS    SS DAK+R+ASMRKL   VVLC++FMS+EVV GIKANSLAIL 
Sbjct: 6   SKVCGET-ACGFS---TSSSDAKKRAASMRKLCFVVVLCLLFMSIEVVCGIKANSLAILA 65

Query: 88  DAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEA 147
           DAAHLL+DV AFAIS+ SLWAS WEA PRQSYGFFRIEILG LVSIQ+IWLL GILVYEA
Sbjct: 66  DAAHLLTDVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEA 125

Query: 148 IVRLINGPG-EVKGLLMFAVSAFGLVVNIAMALLLGHEHGHAHSHGHSHGHGDHDHGHGS 207
           + RL+     +V G  M  V+AFGLVVNI M ++LGH+HGH H HGHS     HDHGH  
Sbjct: 126 VTRLVQETNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHDHGHS-----HDHGHSY 185

Query: 208 HEHGHEHHGISVTTHHHHLHHRKEEEVTTSDGVKDDHHHAHEVSVTAPLLESCCDDENQK 267
            E   +                                          LLE         
Sbjct: 186 GERAEQ------------------------------------------LLEK-------- 245

Query: 268 VTKAQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAAIWYNSEYKILDLICTLIFSAIVLC 327
                K+ RNINVQGAYLHVLGD IQSIGVMIGG  IWYN ++K++DLICTL FS IVL 
Sbjct: 246 ----SKEIRNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNPKWKVIDLICTLFFSVIVLG 305

Query: 328 TTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVII 387
           TTI+MLR+ILEVLMESTPREIDA +LEKGL E++EVV +HELHIWAITVGK L +CHV +
Sbjct: 306 TTIKMLRSILEVLMESTPREIDARQLEKGLMEIEEVVDVHELHIWAITVGKALFSCHVKV 334

Query: 388 KPEANADMVLDKVIEYIRREYNISHVTIQIER 419
           +PEA  +MVL+KVI+YI REY ISHVTIQIER
Sbjct: 366 RPEAGDEMVLNKVIDYIWREYRISHVTIQIER 334