Tan0013015 (gene) Snake gourd v1

Overview


Name	Tan0013015
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	(1->3)-beta-glucan endohydrolase
Location	LG04: 2488923 .. 2492271 (-)
RNA-Seq Expression	Tan0013015
Synteny	Tan0013015

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGTGGTCCTCTCTCGGCCCCCACATCTCTCCGCCGCCGTCCGACCATGTCTGCCGCCGCGGCCTCCGCCGTACTTCTCCTCCTCCTACAAATCTCCACCGCCGTCGTCGCCATTGGCGTCAACTACGGCACTCTGGGGAACAACCTACGGCCGCCGGCAGAGGTGGTGAACTTCATCAGAACGAAAACTAAAATCGACCGCGTGAAGCTCTTCGATGCGAATCCAGACATCCTGCGGGCATTCGCCGGCACAGGAATTCTGGTGACGGTGACCGTCGGGAACGGCGAGATCCCCGCCCTGACGGATCTGGCCGCCGCTCGACAGTGGGTCGCCGCTCATATCGCGCCGTTTCACCCTCAGACGCGGATTAATTACATTGCTGTTGGAAATGAAATCATGCACACCAACGATAAGTCCTTGATCTCCCGTCTCGTTCCCGCCATGAGAACGCTCCACCGAGCTCTGGTTCTCGCCGGAATTATGGACGTTAAGGTAACGGTGTTGATTTTTCTTTTCCTTATTCTTTTTTTTTTTTAGTCGATGAGAAAGCCGTTTTTAGCTGTTGGGTTATAATGAAATCTAATGATTTATTCTTTCGAGTGATATTAAATTAAATTAAAGATGAATTTTAAATGAGTTTTTTTCATTCGGATTTAAAATTTTGTTTTTATTTTTTTTAGTAACGGTTCGGTTGCAATGCAAAGTAAATTTGATTGTAAATGTAATGTATCTTTTTGTATTGGTAAACATTTTATTTTCTTTCCAAATATTTAAAATTGTAGTTGCATTTATATTTATGTCAAACCTTTCTCATTAAAGTAAACAACATTAAATCATCTTAATCATTTTAACTTATGTCAATTTCAAATAAATGGCGTTATGTTGCTAAAAAAGTTATGTTATATGATTTTTTTTAAAATATATAATTCAAGTTGAATTTAAACTTAAATATAACAAGAATTATATTTATGATGCCAACAAGATCATAGCTCAACCTTTGACATCTAACAACGTCGTTTATTCATTAATCAGGTTACATCTCCACATTCTCTAGGCATTCTTTCAATCTCCGAGCCACCCAGCATGGGTCGGTTCAGGCGAGGCTATGATCGAGCCGTCTTTACACCCATGCTCCAGTTCCTACGAGAAACCAAGGCTCCATTCATGGTCAATCCCTATCCCTACTTCGGCTACTCACCAAAAATGGCTAATTACGCCTTGTTCCAACCAAACCGTGGCATTTACGACAAGTTCAACAAAATCACCTACACCAACATGTTCGACGCCACAATGGACGCCATCCACACCGCCATGAAGGCCATCGGCTTCGCCGATGTCGACCTTGTCATCGGCGAGACTGGTTGGCCGACGAACTGCGACGGCTACGTCGCTTGTAGCCTCGAGAATGCTGCTACTTACAACAGGAATTTGATTTGGCATGTCAATTCTGGAAAGGGGACGCCGTTAATGCCAAACCGGCGGTTTGAGACTTACTTGTTTGCGCTGTTTAATGAGAATCTCAAACCCGGTCCGACCGCCGAGCGGAATTGGGGTCTGTTTCAGCCGGATTTTACTCCCGTTTATGATCTTCCTAACATCATGCTCGGTGGACGGGTTAGTCTATTTTTCTCCCCCACTTTCTCTTAATTTTTTTTGGTCCGTCCGAAGGCTTTTTAAAATAACTATTTTGATTTTTTTTTCTTCTCAAAATTTTTATTTCAGTTCTTAATTTAAAAAAAACAATGATTATTTGGTCTTGTATATTATTTTGTCAATTAGTTATCTAATATTTAAAGGACTGTATAGAAAAATTAAAATTATGTTGGATAACTATTTTAGATGTGTTTTCAAAAATCAACTCAAAATTTGAAATTTTAAAAAGTAGTTTCAAAAATTTGTTTTTGTTTTTAAAATTTAACTAAAAACTCGAGTATTTTTTTAAAAAGGTGGGAACTATGGTAGGATAAAGGAATACTATGGTAGGAAAATTGTGAGAAAATAAATATAATTTTTAAAAATTAAAAACCAAATGATTATCAAATTGATTGTTTTTTATTTGTCTCATTTTTCATTTTTTGGAATCGAAATATTAAGTAAATGAAAAGTGATTAGAGTAAGATTTTGCCTTATACTGTAAATTTGTTGGCTTTGCCTCATAATAAATGATCTTATCTAATTATTTAAAGTAAAATTAAAAAAAAAATCGTTTAAAGTTCAGAGATTTTATTTCTAATCCCAAAAAATTTAGGAGTTAAAATATACACCTTGTTAATGGACCAAATGAAATACATAATGATAAATTTGTTTGCTTAAGAGTTTGAGTGATTTGATCAAATTTTTACTGATTTCAAATTGCGTTATCATGGGTTAAAATTTTGTTGATCGAGAAGATTGGATTTTGCAAAATTTGGTAGAATTTTGAGTTAAATTACTCGACTTTATCGTTTTTTTAATTTTATATTAATAAATAAAAAATCAAAATTCTACATTACAAAAACCAACTAATGATGCGAGATTTTATTTTAATTACAGCACGGTGGTCGGAGGACGCCGACGCCGGTGGCGCCATCGGGGAAGAAATGGTGCGTACCGAGATCCGACGCGGCCATTGAAGCCTTACAGAAGAACATCGACTACGTTTGCAGCTCGAACATCGACTGCCGCCCGATCCAAGCCGGCGGCGCCTGCTTTCAGCCCAACGACGTCCGATCCCACGCCGGTTACGTCATGAATTCCTATTATCAGACAAATGGCCGCCACGATTTCAACTGCGATTTCGGTCACACCGGCGTTTTAACCACCGTCAATCCAAGTAATTATAGCTTCTTCTTTTCCTTTCGATTAATTTTCTGAATTGAATTATTTGATCCGAGGACTTTGTGATGTAACCGCCGTGTGCGGTGGCTTACTGGTGGCGATCTGATGCAGGTCACGGCGGGTGTGCATACCTTTCTTGAGAGCTCGAGCGGCGGAGAAGGAATTTTTGTTGCCTACGGGCGAGCACAGGAGAGGAAACTCTGGAGACCACATAAAGCTTCCTAATCAATAGAGCCTGAAAATACTTTTTCCTTTTTGTTTTTTCTTTTCTTTTTTTTTTCTTCCATAAAATGATGTATTTTTCATTTGTTGTAAACTTGTAACTTGTAATTCAATTGGAATCTTCTAGAAAAATCTCGCTCTCATTTTACAAAAGTGAGATTTGGAGATTGCAGAAGATTCCATTAATTATTCAAAACAATATCGTGTCCCTTTTTTGTTCATGGTAATTTTGTAGAATTATCGTTAAACAAATGTTGGGATTCAAATTTTCCCTGAGGTTGCTAATGAATCGATTACTTCAACAACAAA

mRNA sequence

CAGTGGTCCTCTCTCGGCCCCCACATCTCTCCGCCGCCGTCCGACCATGTCTGCCGCCGCGGCCTCCGCCGTACTTCTCCTCCTCCTACAAATCTCCACCGCCGTCGTCGCCATTGGCGTCAACTACGGCACTCTGGGGAACAACCTACGGCCGCCGGCAGAGGTGGTGAACTTCATCAGAACGAAAACTAAAATCGACCGCGTGAAGCTCTTCGATGCGAATCCAGACATCCTGCGGGCATTCGCCGGCACAGGAATTCTGGTGACGGTGACCGTCGGGAACGGCGAGATCCCCGCCCTGACGGATCTGGCCGCCGCTCGACAGTGGGTCGCCGCTCATATCGCGCCGTTTCACCCTCAGACGCGGATTAATTACATTGCTGTTGGAAATGAAATCATGCACACCAACGATAAGTCCTTGATCTCCCGTCTCGTTCCCGCCATGAGAACGCTCCACCGAGCTCTGGTTCTCGCCGGAATTATGGACGTTAAGGTTACATCTCCACATTCTCTAGGCATTCTTTCAATCTCCGAGCCACCCAGCATGGGTCGGTTCAGGCGAGGCTATGATCGAGCCGTCTTTACACCCATGCTCCAGTTCCTACGAGAAACCAAGGCTCCATTCATGGTCAATCCCTATCCCTACTTCGGCTACTCACCAAAAATGGCTAATTACGCCTTGTTCCAACCAAACCGTGGCATTTACGACAAGTTCAACAAAATCACCTACACCAACATGTTCGACGCCACAATGGACGCCATCCACACCGCCATGAAGGCCATCGGCTTCGCCGATGTCGACCTTGTCATCGGCGAGACTGGTTGGCCGACGAACTGCGACGGCTACGTCGCTTGTAGCCTCGAGAATGCTGCTACTTACAACAGGAATTTGATTTGGCATGTCAATTCTGGAAAGGGGACGCCGTTAATGCCAAACCGGCGGTTTGAGACTTACTTGTTTGCGCTGTTTAATGAGAATCTCAAACCCGGTCCGACCGCCGAGCGGAATTGGGGTCTGTTTCAGCCGGATTTTACTCCCGTTTATGATCTTCCTAACATCATGCTCGGTGGACGGCACGGTGGTCGGAGGACGCCGACGCCGGTGGCGCCATCGGGGAAGAAATGGTGCGTACCGAGATCCGACGCGGCCATTGAAGCCTTACAGAAGAACATCGACTACGTTTGCAGCTCGAACATCGACTGCCGCCCGATCCAAGCCGGCGGCGCCTGCTTTCAGCCCAACGACGTCCGATCCCACGCCGGTTACGTCATGAATTCCTATTATCAGACAAATGGCCGCCACGATTTCAACTGCGATTTCGGTCACACCGGCGTTTTAACCACCGTCAATCCAAGTCACGGCGGGTGTGCATACCTTTCTTGAGAGCTCGAGCGGCGGAGAAGGAATTTTTGTTGCCTACGGGCGAGCACAGGAGAGGAAACTCTGGAGACCACATAAAGCTTCCTAATCAATAGAGCCTGAAAATACTTTTTCCTTTTTGTTTTTTCTTTTCTTTTTTTTTTCTTCCATAAAATGATGTATTTTTCATTTGTTGTAAACTTGTAACTTGTAATTCAATTGGAATCTTCTAGAAAAATCTCGCTCTCATTTTACAAAAGTGAGATTTGGAGATTGCAGAAGATTCCATTAATTATTCAAAACAATATCGTGTCCCTTTTTTGTTCATGGTAATTTTGTAGAATTATCGTTAAACAAATGTTGGGATTCAAATTTTCCCTGAGGTTGCTAATGAATCGATTACTTCAACAACAAA

Coding sequence (CDS)

ATGTCTGCCGCCGCGGCCTCCGCCGTACTTCTCCTCCTCCTACAAATCTCCACCGCCGTCGTCGCCATTGGCGTCAACTACGGCACTCTGGGGAACAACCTACGGCCGCCGGCAGAGGTGGTGAACTTCATCAGAACGAAAACTAAAATCGACCGCGTGAAGCTCTTCGATGCGAATCCAGACATCCTGCGGGCATTCGCCGGCACAGGAATTCTGGTGACGGTGACCGTCGGGAACGGCGAGATCCCCGCCCTGACGGATCTGGCCGCCGCTCGACAGTGGGTCGCCGCTCATATCGCGCCGTTTCACCCTCAGACGCGGATTAATTACATTGCTGTTGGAAATGAAATCATGCACACCAACGATAAGTCCTTGATCTCCCGTCTCGTTCCCGCCATGAGAACGCTCCACCGAGCTCTGGTTCTCGCCGGAATTATGGACGTTAAGGTTACATCTCCACATTCTCTAGGCATTCTTTCAATCTCCGAGCCACCCAGCATGGGTCGGTTCAGGCGAGGCTATGATCGAGCCGTCTTTACACCCATGCTCCAGTTCCTACGAGAAACCAAGGCTCCATTCATGGTCAATCCCTATCCCTACTTCGGCTACTCACCAAAAATGGCTAATTACGCCTTGTTCCAACCAAACCGTGGCATTTACGACAAGTTCAACAAAATCACCTACACCAACATGTTCGACGCCACAATGGACGCCATCCACACCGCCATGAAGGCCATCGGCTTCGCCGATGTCGACCTTGTCATCGGCGAGACTGGTTGGCCGACGAACTGCGACGGCTACGTCGCTTGTAGCCTCGAGAATGCTGCTACTTACAACAGGAATTTGATTTGGCATGTCAATTCTGGAAAGGGGACGCCGTTAATGCCAAACCGGCGGTTTGAGACTTACTTGTTTGCGCTGTTTAATGAGAATCTCAAACCCGGTCCGACCGCCGAGCGGAATTGGGGTCTGTTTCAGCCGGATTTTACTCCCGTTTATGATCTTCCTAACATCATGCTCGGTGGACGGCACGGTGGTCGGAGGACGCCGACGCCGGTGGCGCCATCGGGGAAGAAATGGTGCGTACCGAGATCCGACGCGGCCATTGAAGCCTTACAGAAGAACATCGACTACGTTTGCAGCTCGAACATCGACTGCCGCCCGATCCAAGCCGGCGGCGCCTGCTTTCAGCCCAACGACGTCCGATCCCACGCCGGTTACGTCATGAATTCCTATTATCAGACAAATGGCCGCCACGATTTCAACTGCGATTTCGGTCACACCGGCGTTTTAACCACCGTCAATCCAAGTCACGGCGGGTGTGCATACCTTTCTTGA

Protein sequence

MSAAAASAVLLLLLQISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFDANPDILRAFAGTGILVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVGNEIMHTNDKSLISRLVPAMRTLHRALVLAGIMDVKVTSPHSLGILSISEPPSMGRFRRGYDRAVFTPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATMDAIHTAMKAIGFADVDLVIGETGWPTNCDGYVACSLENAATYNRNLIWHVNSGKGTPLMPNRRFETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRRTPTPVAPSGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQTNGRHDFNCDFGHTGVLTTVNPSHGGCAYLS

Homology

[Close]

BLAST of Tan0013015 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 6.5e-129
Identity = 236/458 (51.53%), Postives = 306/458 (66.81%), Query Frame = 0


Query: 9   VLLLLL-------QISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFDANPD 68
           +LL+LL       + +T  + IGVNYG   +NL  P  V  F+ TKT IDRVKLFDANP 
Sbjct: 5   ILLMLLAAGAAGAESATPSLHIGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANPT 64

Query: 69  ILRAFAGTGILVTVTVGNGEIPALTD----LAAARQWVAAHIAPFHPQTRINYIAVGNEI 128
            + AFAGT I + V++ N  +PAL D    L AAR W+ A+++P+ P T +  +  GNEI
Sbjct: 65  FISAFAGTPISLAVSLPNSALPALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNEI 124

Query: 129 MHTNDKSLISRLVPAMRTLHRALVLAGIMDVKVTSPHSLGILSISEP-PSMGRFRRGYDR 188
           + + D +LI  L+PAMR L +AL   G+  V+VT+PH LGIL+ S+  PS   FR GY+ 
Sbjct: 125 LLSTDTNLILSLLPAMRRLAQALKAEGLTGVRVTTPHYLGILAPSDGIPSNASFRAGYNT 184

Query: 189 AVFTPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATM 248
            +F  MLQF R+T +PFMVNPYPYF Y P+  NYALF+PN GIYD   K+ YT+M DA M
Sbjct: 185 KLFPAMLQFHRDTGSPFMVNPYPYFSYRPETLNYALFRPNSGIYDPATKLNYTSMLDAQM 244

Query: 249 DAIHTAMKAIGFADVDLVIGETGWPTNCD-GYVACSLENAATYNRNLIWHVNSGKGTPLM 308
           DAI+TAMK +G+ DVD+ +GE GWPT  + G +   ++ A  +N  +I   +SGKGTPLM
Sbjct: 245 DAIYTAMKKLGYGDVDIAVGEAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTPLM 304

Query: 309 PNRRFETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRRTPTP--- 368
           PNR FETYLF+LF+EN KPGP AER++GLF PDFTPVYDL  +  G       +P P   
Sbjct: 305 PNRTFETYLFSLFDENQKPGPIAERHFGLFNPDFTPVYDLGLLRDGASVAPTPSPNPSPN 364

Query: 369 ----VAPS-GKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGY 428
                APS G KWCV +  A    LQ NI+Y C   +DC+PIQ+GGACF PN +++HA Y
Sbjct: 365 PSPKPAPSGGGKWCVAKDGANGTDLQNNINYACGF-VDCKPIQSGGACFSPNSLQAHASY 424

Query: 429 VMNSYYQTNGRHDFNCDFGHTGVLTTVNPSHGGCAYLS 446
           VMN+YYQ NG  D  CDF  TG++T+ +PS+GGC Y+S
Sbjct: 425 VMNAYYQANGHTDLACDFKGTGIVTSSDPSYGGCKYVS 461

[Close]

BLAST of Tan0013015 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 385.6 bits (989), Expect = 7.8e-106
Identity = 191/429 (44.52%), Postives = 283/429 (65.97%), Query Frame = 0


Query: 23  IGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFDANPDILRAFAGTGILVTVTVGNGEI 82
           IGVNYG + +NL PP+E V  +++ T I +V+L+ A+P I++A AGTG+ + +   NG++
Sbjct: 26  IGVNYGQVADNLPPPSETVKLLQS-TSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDV 85

Query: 83  PAL-TDLAAARQWVAAHIAPFHPQTRINYIAVGNEIMHTNDKSLISRLVPAMRTLHRALV 142
           P+L +D  AA QW+ +++ PF+P ++I  I VGNEI+ +ND +L+++L+PAM+ + +AL 
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALE 145

Query: 143 LAGI-MDVKVTSPHSLGILSISEPPSMGRFRRGYDRAVFTPMLQFLRETKAPFMVNPYPY 202
              +   +KV++ +S+ +L  S+PPS G F  GY   +   +LQFL +T +PF +NPYP+
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGL-KGILQFLSDTGSPFAINPYPF 205

Query: 203 FGYS----PKMANYALFQPNRGIYDKFNKITYTNMFDATMDAIHTAMKAIGFADVDLVIG 262
           F Y     P+   + LF+PN G  D    I YTNMFDA +DA+H+A+K++GF  V++V+ 
Sbjct: 206 FAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVA 265

Query: 263 ETGWPTNCD-GYVACSLENAATYNRNLIWHVNSGKGTPLMPNRRFETYLFALFNENLKPG 322
           ETGW +  D   V  S++NA  YN NLI H+ S  GTPLMP +  +TY+FAL++ENLKPG
Sbjct: 266 ETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPG 325

Query: 323 PTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRRTPT-PVAPSGKKWCVPRSDAAIEALQK 382
           P++ER +GLF+ D + VYD   + L       +TP+  V  SG  WCVP+  A  E LQ 
Sbjct: 326 PSSERAFGLFKTDLSMVYD---VGLAKSSSSSQTPSGKVTSSG--WCVPKKGATNEELQA 385

Query: 383 NIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQTNGRHDFNCDFGHTGVLTTV 442
           ++D+ C   IDC  IQ GGACF+PN+V SHA Y MN Y+Q + +   +CDF  T  +T+ 
Sbjct: 386 SLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQ 445

Query: 443 NPSHGGCAY 444
           NPS+  C Y
Sbjct: 446 NPSYNNCVY 447

[Close]

BLAST of Tan0013015 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 4.0e-102
Identity = 190/430 (44.19%), Postives = 270/430 (62.79%), Query Frame = 0


Query: 23  IGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFDANPDILRAFAGTGILVTVTVGNGEI 82
           +GVNYG L +NL      VN +++ T I +V+LF A P +++AFA TG+ + +   NG+I
Sbjct: 30  LGVNYGQLSDNLPSLQATVNLLKS-TTIQKVRLFGAEPAVIKAFANTGVEIVIGFDNGDI 89

Query: 83  PAL-TDLAAARQWVAAHIAPFHPQTRINYIAVGNEIMHTNDKSLISRLVPAMRTLHRALV 142
           P L ++   A Q+V +++  F+P + I  I VGNE++ + D+ LIS+L+PAM+ +  AL 
Sbjct: 90  PTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSGDQKLISQLLPAMQNVQNALN 149

Query: 143 LAGI-MDVKVTSPHSLGILSISEPPSMGRFRRGYDRAVFTPMLQFLRETKAPFMVNPYPY 202
            A +   VKV++ H++ +LS S PPS G F  G    +   +LQF     APFM++PYPY
Sbjct: 150 AASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGDTM-KALLQFQSANDAPFMISPYPY 209

Query: 203 FGY----SPKMANYALFQPNRGIYDKFNKITYTNMFDATMDAIHTAMKAIGFADVDLVIG 262
           F Y    +P    + LFQPN G  D  N   YTNMFDA +DA+H+A+ A+GF D+++V+ 
Sbjct: 210 FAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVDAVHSALNAMGFKDIEIVVA 269

Query: 263 ETGWPTNCD-GYVACSLENAATYNRNLIWHVNSGKGTPLMPNRRFETYLFALFNENLKPG 322
           ETGWP   D   V  SL+NA  Y  NLI H+ S  GTPLMP +  +TYLF+L++E+ K G
Sbjct: 270 ETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMPGKSIDTYLFSLYDEDKKTG 329

Query: 323 PTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRRTPTPVAPSGKKWCVPRSDAAIEALQKN 382
            ++E+ +GLF+PD +  YD+  +          TPTP   +   WCVP+   + + L  N
Sbjct: 330 ASSEKYFGLFKPDGSTTYDVGLLKNTQNPTTPATPTPTPKAAGSWCVPKPGVSDDQLTGN 389

Query: 383 IDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQTNGRHDFNCDFGHTGVLTTVN 442
           I+Y C   IDC PIQ GGACF+PN V++HA YVMN YYQ+ GR+ +NCDF  T  LT  N
Sbjct: 390 INYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQSAGRNSWNCDFSQTATLTNTN 449

Query: 443 PSHGGCAYLS 446
           PS+G C + S
Sbjct: 450 PSYGACNFPS 457

[Close]

BLAST of Tan0013015 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 4.7e-79
Identity = 169/453 (37.31%), Postives = 243/453 (53.64%), Query Frame = 0


Query: 23  IGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFDANPDILRAFAGTGILVTVTVGNGEI 82
           IG+ YG   +NL  P  V   I+    I  V+++DAN D+L+AFA TGI + + V N ++
Sbjct: 27  IGICYGRNADNLPSPNRVSELIQ-HLNIKFVRIYDANIDVLKAFANTGIELMIGVPNADL 86

Query: 83  PALTDLAA-ARQWVAAHIAPFHPQTRINYIAVGNEIMHTNDKSLISRLVPAMRTLHRALV 142
            A     +    W++ +I P++P T+I  I+VG E+    D +    ++PAMR +H AL 
Sbjct: 87  LAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNA-TGLVLPAMRNIHTALK 146

Query: 143 LAGI-MDVKVTSPHSLGILSISEPPSMGRFRRGYDRAVFTPMLQFLRETKAPFMVNPYPY 202
            +G+   +K++S HSL ILS S PPS   F + +  A   PML+FL E ++PFM++ YPY
Sbjct: 147 KSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHS-AFLKPMLEFLVENESPFMIDLYPY 206

Query: 203 FGYSPKM----ANYALFQPNRGIYDKFNKITYTNMFDATMDAIHTAMKAIGFADVDLVIG 262
           + Y          YALF+ +  + D    + Y+NMFDA +DAI+ A+ A+ F  V +++ 
Sbjct: 207 YAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVT 266

Query: 263 ETGWPT-NCDGYVACSLENAATYNRNLIWHVNSGKGTPLMPNRRFETYLFALFNENLKPG 322
           E+GWP+       A + ENA  YN NLI HV    GTP  P    + YLF+LFNEN KPG
Sbjct: 267 ESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPG 326

Query: 323 PTAERNWGLFQPDFTPVYDL--------------------PNIMLGGRHGGRRTPTPVAP 382
             +ERNWG+F  + T VY L                    P+        G  T T    
Sbjct: 327 IESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGG 386

Query: 383 SG---KKWCVPRSDAAIEALQKNIDYVCS-SNIDCRPIQAGGACFQPNDVRSHAGYVMNS 442
            G   KKWC+  S A++  LQ  +D+ C   N+DC  +Q    CF+P+ V SHA Y  N+
Sbjct: 387 GGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNT 446

Query: 443 YYQTNGRHDFNCDFGHTGVLTTVNPSHGGCAYL 445
           YYQ +G    +C F    V    +PS+G C Y+
Sbjct: 447 YYQQSGASSIDCSFNGASVEVDKDPSYGNCLYM 476

[Close]

BLAST of Tan0013015 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 5.2e-78
Identity = 169/447 (37.81%), Postives = 244/447 (54.59%), Query Frame = 0


Query: 8   AVLLLLLQISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFDANPDILRAFA 67
           ++LLLLL        +GV YG   ++L  P++VV  I+ +  I  V+++D N  +L+AF 
Sbjct: 12  SILLLLLDCCYG-GKVGVCYGRSADDLPTPSKVVQLIQ-QHNIKYVRIYDYNSQVLKAFG 71

Query: 68  GTGILVTVTVGNGEIPALTDLAA-ARQWVAAHIAPFHPQTRINYIAVGNEIMHTNDKSLI 127
            T I + + V N ++ A +   +    W+   + P++P T+I YI VG E       +  
Sbjct: 72  NTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINAS 131

Query: 128 SRLVPAMRTLHRALVLAGI-MDVKVTSPHSLGILSISEPPSMGRFRRGYDRAVFTPMLQF 187
           S +VPAM+ +  AL   G+   +KV++  SLGILS S PPS G F   Y      PML+F
Sbjct: 132 SFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSY-AYFLRPMLEF 191

Query: 188 LRETKAPFMVNPYPYFGYSPKMAN----YALFQPNRGIYDKFNKITYTNMFDATMDAIHT 247
           L E K+PFM++ YPY+ Y     N    Y LF+ +  + D    + Y NMFDA +DA++ 
Sbjct: 192 LAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYY 251

Query: 248 AMKAIGFADVDLVIGETGWPT--NCDGYVACSLENAATYNRNLIWHVNSGKGTPLMPNRR 307
           A+ A+ F  + +++ ETGWPT  +     A S +NA TYN N+I HV + +GTP  P   
Sbjct: 252 ALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEA 311

Query: 308 FETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRRTPTPVAPSGKK 367
              Y+F+LFNEN K G  +ERNWGLF PD T VY L             + T  + S   
Sbjct: 312 MNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDFTGKSNGFHSNSSGTNSSGSSNS 371

Query: 368 WCVPRSDAAIEALQKNIDYVCS-SNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQTNGR 427
           WC+  S A+   L+  +D+ C   N+DC  IQ    CFQP+ + SHA +V NSY+Q N  
Sbjct: 372 WCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRA 431

Query: 428 HDFNCDFGHTGVLTTVNPSHGGCAYLS 446
            D  C FG  GV    +PS+  C Y++
Sbjct: 432 TDVACSFGGAGVKVNKDPSYDKCIYIT 455

[Close]

BLAST of Tan0013015 vs. NCBI nr
Match: XP_038891662.1 (glucan endo-1,3-beta-glucosidase, partial [Benincasa hispida])

HSP 1 Score: 818.9 bits (2114), Expect = 2.1e-233
Identity = 399/455 (87.69%), Postives = 423/455 (92.97%), Query Frame = 0


Query: 1   MSAAAA----SAVLLLLLQISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLF 60
           MS+AAA    SA L LLLQISTAVVAIGVNYGTL NNLRPPAEVVNF++TKTKIDRVK+F
Sbjct: 55  MSSAAAFHRISAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIF 114

Query: 61  DANPDILRAFAGTGILVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVGNE 120
           D NPDILRAFAG+GILVTVTVGNGEIPAL DLA ARQWVAAHIAPFHP+TRI+YIAVGNE
Sbjct: 115 DTNPDILRAFAGSGILVTVTVGNGEIPALGDLAVARQWVAAHIAPFHPRTRIHYIAVGNE 174

Query: 121 IMHTNDKSLISRLVPAMRTLHRALVLAGIMDVKVTSPHSLGILSISEPPSMGRFRRGYDR 180
           I+H+NDK LI+RLVPAMR+LHRALVLAGI DVKVTSPHSLGILSISEPPSMGRFRRGYDR
Sbjct: 175 IIHSNDKPLIARLVPAMRSLHRALVLAGIRDVKVTSPHSLGILSISEPPSMGRFRRGYDR 234

Query: 181 AVFTPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATM 240
            +F PMLQFLRETKAPFMVNPYPYFGYSPKMANYALF+PNRGIYDK+ KITYTNMFD  M
Sbjct: 235 TIFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFRPNRGIYDKYTKITYTNMFDGMM 294

Query: 241 DAIHTAMKAIGFADVDLVIGETGWPTNCDGYVACSLENAATYNRNLIWHVNSGKGTPLMP 300
           DA+++AMK +G+ADVDLVIGETGWPTNCD Y+ACS +NAATYNRNLIWHVNSGKGTPLMP
Sbjct: 295 DALYSAMKKLGYADVDLVIGETGWPTNCD-YIACSPDNAATYNRNLIWHVNSGKGTPLMP 354

Query: 301 NRRFETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDLPNIML----GGRHGGRRT--P 360
           NR+FETYLF LFNENLKPGPTAERNWGLFQPDFTPVYDLPNIML    GGR GGRRT  P
Sbjct: 355 NRKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGRRRGGRGGGRRTPSP 414

Query: 361 TPVAPSGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMN 420
           TPVAPSG+KWCVPRSDA IEALQKNIDYVCSSNIDCRPIQAGGACF PNDVRSHA YVMN
Sbjct: 415 TPVAPSGQKWCVPRSDAPIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMN 474

Query: 421 SYYQTNGRHDFNCDFGHTGVLTTVNPSHGGCAYLS 446
           SYYQT GRHDFNCDFGHTGVLTTVNPSHGGCAY+S
Sbjct: 475 SYYQTAGRHDFNCDFGHTGVLTTVNPSHGGCAYVS 508

[Close]

BLAST of Tan0013015 vs. NCBI nr
Match: XP_022992969.1 (glucan endo-1,3-beta-glucosidase [Cucurbita maxima])

HSP 1 Score: 802.4 bits (2071), Expect = 2.0e-228
Identity = 382/450 (84.89%), Postives = 415/450 (92.22%), Query Frame = 0


Query: 1   MSAAA---ASAVLLLLLQISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFD 60
           MSAAA     A LL+LLQISTA VAIGVNYGTL NNLRPPAEVVNFI+TKTKIDRVK+FD
Sbjct: 1   MSAAAFHRLFAALLILLQISTAAVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFD 60

Query: 61  ANPDILRAFAGTGILVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVGNEI 120
            NPDILRAFAGTGILVTVTVGNGEIPALTDLA ARQWVAAHI PFHPQTRINYIAVGNEI
Sbjct: 61  TNPDILRAFAGTGILVTVTVGNGEIPALTDLATARQWVAAHIRPFHPQTRINYIAVGNEI 120

Query: 121 MHTNDKSLISRLVPAMRTLHRALVLAGIMDVKVTSPHSLGILSISEPPSMGRFRRGYDRA 180
           +HTNDK LI+RLVPAM++LHRALVLAGI D+KVTSPHSL ILSISEPPSMGRFRRGYDRA
Sbjct: 121 IHTNDKPLIARLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSMGRFRRGYDRA 180

Query: 181 VFTPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATMD 240
           +F PMLQFLRETKAPFMVNPYPYFGYSP MANYALF PNRG+YDK+ KITYTNMFDA MD
Sbjct: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMD 240

Query: 241 AIHTAMKAIGFADVDLVIGETGWPTNCDGYVACSLENAATYNRNLIWHVNSGKGTPLMPN 300
           A+++AMK IG++DVDLVIGETGWP+NCDGYVACS  NAAT++RNL+WHVNSGKGTPLMPN
Sbjct: 241 ALYSAMKNIGYSDVDLVIGETGWPSNCDGYVACSPGNAATFHRNLVWHVNSGKGTPLMPN 300

Query: 301 RRFETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRR--TPTPVAP 360
           R+FETYLFALFNENLKPGPTAERNWGLF PDFTPVYD+PNIM+GGRH G R   P PVAP
Sbjct: 301 RKFETYLFALFNENLKPGPTAERNWGLFHPDFTPVYDVPNIMIGGRHRGGRGGRPKPVAP 360

Query: 361 SGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQT 420
           SGKKWCVP+SDA+++ALQKNIDYVCSSNIDCRPIQAGGACF PND+R+HA Y MNSYYQT
Sbjct: 361 SGKKWCVPKSDASVQALQKNIDYVCSSNIDCRPIQAGGACFLPNDIRAHASYAMNSYYQT 420

Query: 421 NGRHDFNCDFGHTGVLTTVNPSHGGCAYLS 446
           +GR+DFNCDF  TGV+TTVNPSHGGC Y+S
Sbjct: 421 SGRNDFNCDFSLTGVITTVNPSHGGCVYIS 450

[Close]

BLAST of Tan0013015 vs. NCBI nr
Match: XP_004147695.2 (glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KGN50054.2 hypothetical protein Csa_021427 [Cucumis sativus])

HSP 1 Score: 801.2 bits (2068), Expect = 4.5e-228
Identity = 384/447 (85.91%), Postives = 415/447 (92.84%), Query Frame = 0


Query: 1   MSAAA---ASAVLLLLLQIS-TAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLF 60
           MSAAA    SA L LLLQIS TAV AIGVNYGTLGNNLRPPAEV NF++TKTKIDRVK+F
Sbjct: 1   MSAAALRRLSAALFLLLQISTTAVFAIGVNYGTLGNNLRPPAEVANFLKTKTKIDRVKIF 60

Query: 61  DANPDILRAFAGTGILVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVGNE 120
           D NPDILRAFAG+GILVTVTVGNG IP L DLA AR+WVAAHIAPFHPQTRI+YIAVGNE
Sbjct: 61  DTNPDILRAFAGSGILVTVTVGNGLIPNLADLAFAREWVAAHIAPFHPQTRIHYIAVGNE 120

Query: 121 IMHTNDKSLISRLVPAMRTLHRALVLAGIMDVKVTSPHSLGILSISEPPSMGRFRRGYDR 180
           I+H+NDK LI+RLVPAMR+LHRALVLAGI DVKVTSPHSLGILSISEPPSMGRFRRGYDR
Sbjct: 121 IIHSNDKPLIARLVPAMRSLHRALVLAGIRDVKVTSPHSLGILSISEPPSMGRFRRGYDR 180

Query: 181 AVFTPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATM 240
           A+F PMLQFLRETKAPFMVNPYPYFGYSPKMANY LF+PNRGIYDK+ KITYTNMFD+ M
Sbjct: 181 AIFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTKITYTNMFDSMM 240

Query: 241 DAIHTAMKAIGFADVDLVIGETGWPTNCDGYVACSLENAATYNRNLIWHVNSGKGTPLMP 300
           DAI++AMK +G+ADV++V+GETGWPTNCD Y+ACS +NAATYNRNL+WHVNSGKGTPLMP
Sbjct: 241 DAIYSAMKKLGYADVNIVLGETGWPTNCD-YIACSPDNAATYNRNLVWHVNSGKGTPLMP 300

Query: 301 NRRFETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRRTPTPVAPS 360
           NR+FETYLF LFNENLKPGPTAERNWGLFQPDFTPVY LPNIMLGGR  G R PTPVAPS
Sbjct: 301 NRKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYSLPNIMLGGRGRGGRRPTPVAPS 360

Query: 361 GKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQTN 420
           GKKWCVPRSDAA++ALQKNIDYVCSSN+DCRPIQAGGACF PNDVRSHA YVMNSYYQT+
Sbjct: 361 GKKWCVPRSDAAVDALQKNIDYVCSSNVDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTS 420

Query: 421 GRHDFNCDFGHTGVLTTVNPSHGGCAY 444
           GRHD+NCDF HTGVLTTVNPSHGGCA+
Sbjct: 421 GRHDYNCDFSHTGVLTTVNPSHGGCAF 446

[Close]

BLAST of Tan0013015 vs. NCBI nr
Match: KAG6601002.1 (Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 800.8 bits (2067), Expect = 5.9e-228
Identity = 382/450 (84.89%), Postives = 415/450 (92.22%), Query Frame = 0


Query: 1   MSAAA---ASAVLLLLLQISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFD 60
           MSAAA     A LL+LLQISTA VAIGVNYGTLGNNLRPPAEVVNFI+TKTKIDRVK+FD
Sbjct: 1   MSAAAFHRLFAALLILLQISTAAVAIGVNYGTLGNNLRPPAEVVNFIKTKTKIDRVKIFD 60

Query: 61  ANPDILRAFAGTGILVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVGNEI 120
            NPDILRAFAGTGILVTVTVGNGEIP LTDLA ARQWVAAHI PFHPQTRI+YIAVGNEI
Sbjct: 61  TNPDILRAFAGTGILVTVTVGNGEIPGLTDLATARQWVAAHIRPFHPQTRIHYIAVGNEI 120

Query: 121 MHTNDKSLISRLVPAMRTLHRALVLAGIMDVKVTSPHSLGILSISEPPSMGRFRRGYDRA 180
           +HTNDK LI+RLVPAM++LHRALVLAGI D+KVTSPHSL ILSISEPPS+GRFRRGYDRA
Sbjct: 121 IHTNDKPLIARLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSLGRFRRGYDRA 180

Query: 181 VFTPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATMD 240
           +F PMLQFLRETKAPFMVNPYPYFGYSP MANYALF PNRG+YDK+ KITYTNMFDA MD
Sbjct: 181 IFAPMLQFLRETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMD 240

Query: 241 AIHTAMKAIGFADVDLVIGETGWPTNCDGYVACSLENAATYNRNLIWHVNSGKGTPLMPN 300
           A+++AMK IG++DVDLVIGETGWPTNCD YVACS  NAAT++RNL+WHVNSGKGTPLMPN
Sbjct: 241 ALYSAMKNIGYSDVDLVIGETGWPTNCD-YVACSPNNAATFHRNLVWHVNSGKGTPLMPN 300

Query: 301 RRFETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRR--TPTPVAP 360
           R+FETYLFALFNENLKPGPTAERNWGLF PDFTPVYD+PNIM+GGRH G R   P PVAP
Sbjct: 301 RKFETYLFALFNENLKPGPTAERNWGLFYPDFTPVYDVPNIMIGGRHRGGRGGGPKPVAP 360

Query: 361 SGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQT 420
           SGKKWCVP+SDA+++ALQKNIDYVCSSNIDCRPIQAGGACF PND+RSHA Y MNSYYQT
Sbjct: 361 SGKKWCVPKSDASVQALQKNIDYVCSSNIDCRPIQAGGACFLPNDIRSHASYAMNSYYQT 420

Query: 421 NGRHDFNCDFGHTGVLTTVNPSHGGCAYLS 446
           +GR+DFNCDF HTGV+TTVNPSHGGC Y+S
Sbjct: 421 SGRNDFNCDFSHTGVITTVNPSHGGCVYIS 449

[Close]

BLAST of Tan0013015 vs. NCBI nr
Match: XP_022957586.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita moschata])

HSP 1 Score: 798.5 bits (2061), Expect = 2.9e-227
Identity = 381/450 (84.67%), Postives = 414/450 (92.00%), Query Frame = 0


Query: 1   MSAAA---ASAVLLLLLQISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFD 60
           MSAAA     A LL+LLQISTA VAIGVNYGTLGNNLRPPAEVVNFI+TKTKIDRVK+FD
Sbjct: 1   MSAAAFHRLFAALLILLQISTAAVAIGVNYGTLGNNLRPPAEVVNFIKTKTKIDRVKIFD 60

Query: 61  ANPDILRAFAGTGILVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVGNEI 120
            NPDILRAFAG+GILVTVTVGNGEIP LTDLA ARQWVAAHI PFHPQTRI+YIAVGNEI
Sbjct: 61  TNPDILRAFAGSGILVTVTVGNGEIPGLTDLATARQWVAAHIRPFHPQTRIHYIAVGNEI 120

Query: 121 MHTNDKSLISRLVPAMRTLHRALVLAGIMDVKVTSPHSLGILSISEPPSMGRFRRGYDRA 180
           +HTNDK LI+RLVPAM++LHRALVLAGI D+KVTSPHSL ILSISEPPSMGRFRRGYDRA
Sbjct: 121 IHTNDKPLIARLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSMGRFRRGYDRA 180

Query: 181 VFTPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATMD 240
           +F PMLQFLRETKAPFMVNPYPYFGYSP MANYALF PNRG+YDK+ KITYTNMFDA MD
Sbjct: 181 IFAPMLQFLRETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMD 240

Query: 241 AIHTAMKAIGFADVDLVIGETGWPTNCDGYVACSLENAATYNRNLIWHVNSGKGTPLMPN 300
           A+++AMK IG++DVDLVIGETGWPTNCD YVACS  NAAT++RNL+WHVNSGKGTPLMPN
Sbjct: 241 ALYSAMKNIGYSDVDLVIGETGWPTNCD-YVACSPNNAATFHRNLVWHVNSGKGTPLMPN 300

Query: 301 RRFETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRR--TPTPVAP 360
           R+FETYLFALFNENLKPGPTAERNWGLF PDFTPVYD+PNIM+GGRH G R   P PVAP
Sbjct: 301 RKFETYLFALFNENLKPGPTAERNWGLFYPDFTPVYDVPNIMIGGRHRGGRGGGPKPVAP 360

Query: 361 SGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQT 420
           SGKKWCVP+SDA+++ALQ NIDYVCSSNIDCRPIQAGGACF PND+RSHA Y MNSYYQT
Sbjct: 361 SGKKWCVPKSDASVQALQNNIDYVCSSNIDCRPIQAGGACFLPNDIRSHASYAMNSYYQT 420

Query: 421 NGRHDFNCDFGHTGVLTTVNPSHGGCAYLS 446
           +GR+DFNCDF HTGV+TTVNPSHGGC Y+S
Sbjct: 421 SGRNDFNCDFSHTGVITTVNPSHGGCVYIS 449

[Close]

BLAST of Tan0013015 vs. ExPASy TrEMBL
Match: A0A6J1JYX6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111489139 PE=3 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 9.8e-229
Identity = 382/450 (84.89%), Postives = 415/450 (92.22%), Query Frame = 0


Query: 1   MSAAA---ASAVLLLLLQISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFD 60
           MSAAA     A LL+LLQISTA VAIGVNYGTL NNLRPPAEVVNFI+TKTKIDRVK+FD
Sbjct: 1   MSAAAFHRLFAALLILLQISTAAVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFD 60

Query: 61  ANPDILRAFAGTGILVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVGNEI 120
            NPDILRAFAGTGILVTVTVGNGEIPALTDLA ARQWVAAHI PFHPQTRINYIAVGNEI
Sbjct: 61  TNPDILRAFAGTGILVTVTVGNGEIPALTDLATARQWVAAHIRPFHPQTRINYIAVGNEI 120

Query: 121 MHTNDKSLISRLVPAMRTLHRALVLAGIMDVKVTSPHSLGILSISEPPSMGRFRRGYDRA 180
           +HTNDK LI+RLVPAM++LHRALVLAGI D+KVTSPHSL ILSISEPPSMGRFRRGYDRA
Sbjct: 121 IHTNDKPLIARLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSMGRFRRGYDRA 180

Query: 181 VFTPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATMD 240
           +F PMLQFLRETKAPFMVNPYPYFGYSP MANYALF PNRG+YDK+ KITYTNMFDA MD
Sbjct: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMD 240

Query: 241 AIHTAMKAIGFADVDLVIGETGWPTNCDGYVACSLENAATYNRNLIWHVNSGKGTPLMPN 300
           A+++AMK IG++DVDLVIGETGWP+NCDGYVACS  NAAT++RNL+WHVNSGKGTPLMPN
Sbjct: 241 ALYSAMKNIGYSDVDLVIGETGWPSNCDGYVACSPGNAATFHRNLVWHVNSGKGTPLMPN 300

Query: 301 RRFETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRR--TPTPVAP 360
           R+FETYLFALFNENLKPGPTAERNWGLF PDFTPVYD+PNIM+GGRH G R   P PVAP
Sbjct: 301 RKFETYLFALFNENLKPGPTAERNWGLFHPDFTPVYDVPNIMIGGRHRGGRGGRPKPVAP 360

Query: 361 SGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQT 420
           SGKKWCVP+SDA+++ALQKNIDYVCSSNIDCRPIQAGGACF PND+R+HA Y MNSYYQT
Sbjct: 361 SGKKWCVPKSDASVQALQKNIDYVCSSNIDCRPIQAGGACFLPNDIRAHASYAMNSYYQT 420

Query: 421 NGRHDFNCDFGHTGVLTTVNPSHGGCAYLS 446
           +GR+DFNCDF  TGV+TTVNPSHGGC Y+S
Sbjct: 421 SGRNDFNCDFSLTGVITTVNPSHGGCVYIS 450

[Close]

BLAST of Tan0013015 vs. ExPASy TrEMBL
Match: A0A6J1GZM3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111458933 PE=3 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 1.4e-227
Identity = 381/450 (84.67%), Postives = 414/450 (92.00%), Query Frame = 0


Query: 1   MSAAA---ASAVLLLLLQISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFD 60
           MSAAA     A LL+LLQISTA VAIGVNYGTLGNNLRPPAEVVNFI+TKTKIDRVK+FD
Sbjct: 1   MSAAAFHRLFAALLILLQISTAAVAIGVNYGTLGNNLRPPAEVVNFIKTKTKIDRVKIFD 60

Query: 61  ANPDILRAFAGTGILVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVGNEI 120
            NPDILRAFAG+GILVTVTVGNGEIP LTDLA ARQWVAAHI PFHPQTRI+YIAVGNEI
Sbjct: 61  TNPDILRAFAGSGILVTVTVGNGEIPGLTDLATARQWVAAHIRPFHPQTRIHYIAVGNEI 120

Query: 121 MHTNDKSLISRLVPAMRTLHRALVLAGIMDVKVTSPHSLGILSISEPPSMGRFRRGYDRA 180
           +HTNDK LI+RLVPAM++LHRALVLAGI D+KVTSPHSL ILSISEPPSMGRFRRGYDRA
Sbjct: 121 IHTNDKPLIARLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSMGRFRRGYDRA 180

Query: 181 VFTPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATMD 240
           +F PMLQFLRETKAPFMVNPYPYFGYSP MANYALF PNRG+YDK+ KITYTNMFDA MD
Sbjct: 181 IFAPMLQFLRETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMD 240

Query: 241 AIHTAMKAIGFADVDLVIGETGWPTNCDGYVACSLENAATYNRNLIWHVNSGKGTPLMPN 300
           A+++AMK IG++DVDLVIGETGWPTNCD YVACS  NAAT++RNL+WHVNSGKGTPLMPN
Sbjct: 241 ALYSAMKNIGYSDVDLVIGETGWPTNCD-YVACSPNNAATFHRNLVWHVNSGKGTPLMPN 300

Query: 301 RRFETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRR--TPTPVAP 360
           R+FETYLFALFNENLKPGPTAERNWGLF PDFTPVYD+PNIM+GGRH G R   P PVAP
Sbjct: 301 RKFETYLFALFNENLKPGPTAERNWGLFYPDFTPVYDVPNIMIGGRHRGGRGGGPKPVAP 360

Query: 361 SGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQT 420
           SGKKWCVP+SDA+++ALQ NIDYVCSSNIDCRPIQAGGACF PND+RSHA Y MNSYYQT
Sbjct: 361 SGKKWCVPKSDASVQALQNNIDYVCSSNIDCRPIQAGGACFLPNDIRSHASYAMNSYYQT 420

Query: 421 NGRHDFNCDFGHTGVLTTVNPSHGGCAYLS 446
           +GR+DFNCDF HTGV+TTVNPSHGGC Y+S
Sbjct: 421 SGRNDFNCDFSHTGVITTVNPSHGGCVYIS 449

[Close]

BLAST of Tan0013015 vs. ExPASy TrEMBL
Match: A0A5D3BZM0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00090 PE=3 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 5.0e-225
Identity = 376/450 (83.56%), Postives = 413/450 (91.78%), Query Frame = 0


Query: 1   MSAAA---ASAVLLLLLQISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFD 60
           MSAA     SA + LLLQIST V AIGVNYGTLGNNLRPPAEVVNF++TKTKIDRVK+FD
Sbjct: 1   MSAATLPRLSAAIFLLLQISTTVFAIGVNYGTLGNNLRPPAEVVNFLKTKTKIDRVKIFD 60

Query: 61  ANPDILRAFAGTGILVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVGNEI 120
            NPDILRAFAG+GILVTVTVGNGEIPAL +LA AR WVA HIAPFHPQTRI+YIAVGNEI
Sbjct: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLAFARAWVAEHIAPFHPQTRIHYIAVGNEI 120

Query: 121 MHTNDKSLISRLVPAMRTLHRALVLAGIMDVKVTSPHSLGILSISEPPSMGRFRRGYDRA 180
           +H+NDK LI+RLVPAMR+LHRALVLAGI D+KVTSPHSLGILSISEPPSMGRFRRGYDRA
Sbjct: 121 IHSNDKPLIARLVPAMRSLHRALVLAGIWDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180

Query: 181 VFTPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATMD 240
           +F PMLQFLRETKAPFMVNPYPYFGYSPKMANY LF+PNRGIYDK+ +ITYTNMFD+ MD
Sbjct: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTRITYTNMFDSMMD 240

Query: 241 AIHTAMKAIGFADVDLVIGETGWPTNCDGYVACSLENAATYNRNLIWHVNSGKGTPLMPN 300
           AI++AMK +G+AD+ +VIGETGWPTNCD Y+ACS +NAAT+NRNL+WHVNSGKGTPLMPN
Sbjct: 241 AIYSAMKKLGYADIKIVIGETGWPTNCD-YIACSPDNAATFNRNLVWHVNSGKGTPLMPN 300

Query: 301 RRFETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLG--GRHGGRRTPTPVAP 360
           R+FETYLF LFNENLKPGPTAERNWGLFQPDFTPVY+LPNIMLG  GR G R +PTP+AP
Sbjct: 301 RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYNLPNIMLGGRGRRGRRPSPTPIAP 360

Query: 361 SGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQT 420
           S KKWCVPRS AA+EALQKNIDYVCSS +DCRPIQAGGACF PNDVRSHA YVMN+YYQT
Sbjct: 361 SAKKWCVPRSGAAVEALQKNIDYVCSSKVDCRPIQAGGACFLPNDVRSHAAYVMNAYYQT 420

Query: 421 NGRHDFNCDFGHTGVLTTVNPSHGGCAYLS 446
           +GRHD+NCDFG TGVLTTVNPSHGGC Y+S
Sbjct: 421 SGRHDYNCDFGQTGVLTTVNPSHGGCVYVS 449

[Close]

BLAST of Tan0013015 vs. ExPASy TrEMBL
Match: A0A1S3CF05 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103500207 PE=3 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 5.0e-225
Identity = 376/450 (83.56%), Postives = 413/450 (91.78%), Query Frame = 0


Query: 1   MSAAA---ASAVLLLLLQISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFD 60
           MSAA     SA + LLLQIST V AIGVNYGTLGNNLRPPAEVVNF++TKTKIDRVK+FD
Sbjct: 1   MSAATLPRLSAAIFLLLQISTTVFAIGVNYGTLGNNLRPPAEVVNFLKTKTKIDRVKIFD 60

Query: 61  ANPDILRAFAGTGILVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVGNEI 120
            NPDILRAFAG+GILVTVTVGNGEIPAL +LA AR WVA HIAPFHPQTRI+YIAVGNEI
Sbjct: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLAFARAWVAEHIAPFHPQTRIHYIAVGNEI 120

Query: 121 MHTNDKSLISRLVPAMRTLHRALVLAGIMDVKVTSPHSLGILSISEPPSMGRFRRGYDRA 180
           +H+NDK LI+RLVPAMR+LHRALVLAGI D+KVTSPHSLGILSISEPPSMGRFRRGYDRA
Sbjct: 121 IHSNDKPLIARLVPAMRSLHRALVLAGIWDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180

Query: 181 VFTPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATMD 240
           +F PMLQFLRETKAPFMVNPYPYFGYSPKMANY LF+PNRGIYDK+ +ITYTNMFD+ MD
Sbjct: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTRITYTNMFDSMMD 240

Query: 241 AIHTAMKAIGFADVDLVIGETGWPTNCDGYVACSLENAATYNRNLIWHVNSGKGTPLMPN 300
           AI++AMK +G+AD+ +VIGETGWPTNCD Y+ACS +NAAT+NRNL+WHVNSGKGTPLMPN
Sbjct: 241 AIYSAMKKLGYADIKIVIGETGWPTNCD-YIACSPDNAATFNRNLVWHVNSGKGTPLMPN 300

Query: 301 RRFETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLG--GRHGGRRTPTPVAP 360
           R+FETYLF LFNENLKPGPTAERNWGLFQPDFTPVY+LPNIMLG  GR G R +PTP+AP
Sbjct: 301 RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYNLPNIMLGGRGRRGRRPSPTPIAP 360

Query: 361 SGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQT 420
           S KKWCVPRS AA+EALQKNIDYVCSS +DCRPIQAGGACF PNDVRSHA YVMN+YYQT
Sbjct: 361 SAKKWCVPRSGAAVEALQKNIDYVCSSKVDCRPIQAGGACFLPNDVRSHAAYVMNAYYQT 420

Query: 421 NGRHDFNCDFGHTGVLTTVNPSHGGCAYLS 446
           +GRHD+NCDFG TGVLTTVNPSHGGC Y+S
Sbjct: 421 SGRHDYNCDFGQTGVLTTVNPSHGGCVYVS 449

[Close]

BLAST of Tan0013015 vs. ExPASy TrEMBL
Match: A0A0A0KS71 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G181480 PE=3 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.5e-224
Identity = 379/441 (85.94%), Postives = 409/441 (92.74%), Query Frame = 0


Query: 1   MSAAA---ASAVLLLLLQIS-TAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLF 60
           MSAAA    SA L LLLQIS TAV AIGVNYGTLGNNLRPPAEV NF++TKTKIDRVK+F
Sbjct: 1   MSAAALRRLSAALFLLLQISTTAVFAIGVNYGTLGNNLRPPAEVANFLKTKTKIDRVKIF 60

Query: 61  DANPDILRAFAGTGILVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVGNE 120
           D NPDILRAFAG+GILVTVTVGNG IP L DLA AR+WVAAHIAPFHPQTRI+YIAVGNE
Sbjct: 61  DTNPDILRAFAGSGILVTVTVGNGLIPNLADLAFAREWVAAHIAPFHPQTRIHYIAVGNE 120

Query: 121 IMHTNDKSLISRLVPAMRTLHRALVLAGIMDVKVTSPHSLGILSISEPPSMGRFRRGYDR 180
           I+H+NDK LI+RLVPAMR+LHRALVLAGI DVKVTSPHSLGILSISEPPSMGRFRRGYDR
Sbjct: 121 IIHSNDKPLIARLVPAMRSLHRALVLAGIRDVKVTSPHSLGILSISEPPSMGRFRRGYDR 180

Query: 181 AVFTPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATM 240
           A+F PMLQFLRETKAPFMVNPYPYFGYSPKMANY LF+PNRGIYDK+ KITYTNMFD+ M
Sbjct: 181 AIFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTKITYTNMFDSMM 240

Query: 241 DAIHTAMKAIGFADVDLVIGETGWPTNCDGYVACSLENAATYNRNLIWHVNSGKGTPLMP 300
           DAI++AMK +G+ADV++V+GETGWPTNCD Y+ACS +NAATYNRNL+WHVNSGKGTPLMP
Sbjct: 241 DAIYSAMKKLGYADVNIVLGETGWPTNCD-YIACSPDNAATYNRNLVWHVNSGKGTPLMP 300

Query: 301 NRRFETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRRTPTPVAPS 360
           NR+FETYLF LFNENLKPGPTAERNWGLFQPDFTPVY LPNIMLGGR  G R PTPVAPS
Sbjct: 301 NRKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYSLPNIMLGGRGRGGRRPTPVAPS 360

Query: 361 GKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQTN 420
           GKKWCVPRSDAA++ALQKNIDYVCSSN+DCRPIQAGGACF PNDVRSHA YVMNSYYQT+
Sbjct: 361 GKKWCVPRSDAAVDALQKNIDYVCSSNVDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTS 420

Query: 421 GRHDFNCDFGHTGVLTTVNPS 438
           GRHD+NCDF HTGVLTTVNPS
Sbjct: 421 GRHDYNCDFSHTGVLTTVNPS 440

[Close]

BLAST of Tan0013015 vs. TAIR 10
Match: AT3G55430.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 508.4 bits (1308), Expect = 5.6e-144
Identity = 256/454 (56.39%), Postives = 331/454 (72.91%), Query Frame = 0


Query: 1   MSAAAASAVLLLL------LQISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVK 60
           M+ A  S  LLLL      L I   + AIGVNYGTLG NL PP +V NFI+T+T ID VK
Sbjct: 1   MAKAPPSISLLLLLCAAVFLTIPAVISAIGVNYGTLG-NLPPPTQVANFIKTQTSIDSVK 60

Query: 61  LFDANPDILRAFAGTGILVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVG 120
           +FD NPDILRAFAGTGI V VTV NG+IPAL +   AR+WV+ +I PFHPQT+I YI+VG
Sbjct: 61  IFDVNPDILRAFAGTGISVVVTVPNGDIPALANGRQARRWVSVNILPFHPQTKIKYISVG 120

Query: 121 NEIMHTNDKSLISRLVPAMRTLHRALVLAGIMDVKVTSPHSLGILS--ISEPPSMGRFRR 180
           NEI+ T D ++I+ L+PAMR L+ ALV AG+ DVKVT+ HSL I++  ++  PS GRFR 
Sbjct: 121 NEILLTGDNNMINNLLPAMRNLNNALVRAGVRDVKVTTAHSLNIIAYDLTGAPSSGRFRP 180

Query: 181 GYDRAVFTPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQ-PNRGIYDKFNKITYTNM 240
           G+D+ +  P+L + R TK+PFMVNPYPYFG+ PK  N+A+F+ P + + D F +  YTNM
Sbjct: 181 GWDKGILAPILAYHRRTKSPFMVNPYPYFGFDPKNVNFAIFRTPYKAVRDPFTRHVYTNM 240

Query: 241 FDATMDAIHTAMKAIGFADVDLVIGETGWPTNCDGYVACSLENAATYNRNLIWHVNSGKG 300
           FDA MD+ ++AMKA+G+ DV++V+GETGWP+ CD    CS  NAA +N N+I     G+G
Sbjct: 241 FDALMDSTYSAMKALGYGDVNIVVGETGWPSACDA-PWCSPANAAWFNLNIIKRA-QGQG 300

Query: 301 TPLMPNRRFETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRRTPT 360
           TPLMPNRRFETY+F LFNE  KPGPTAERNWGLF+ DF+PVYD+  ++  G+ GG R   
Sbjct: 301 TPLMPNRRFETYIFGLFNEEGKPGPTAERNWGLFRADFSPVYDV-GLLRNGQGGGGRPAL 360

Query: 361 PVAPS--GKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVM 420
           P APS  G KWCV RS A    LQ +I++VC   +DC+PIQAGG+CF P+ +R+HA +VM
Sbjct: 361 P-APSTAGGKWCVARSGATNTQLQDSINWVCGQGVDCKPIQAGGSCFNPSSLRTHASFVM 420

Query: 421 NSYYQTNGRHDFNCDFGHTGVLTTVNPSHGGCAY 444
           N+Y+Q++GR D  C+F  TG++   NPS+G C Y
Sbjct: 421 NAYFQSHGRTDGACNFSGTGMIVGNNPSNGACKY 449

[Close]

BLAST of Tan0013015 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 478.8 bits (1231), Expect = 4.8e-135
Identity = 230/448 (51.34%), Postives = 317/448 (70.76%), Query Frame = 0


Query: 2   SAAAASAVLLLLLQISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFDANPD 61
           S +  + V +LL+  + +  +IGVNYGTL NNL PP +V  F+   T I+R++LFD +P 
Sbjct: 9   SLSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQ 68

Query: 62  ILRAFAGTGILVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVGNEIMHTN 121
           IL+AFA TGI VTVTV N +IP LT+L++A+QW++ HI P  P T I  I VGNE++ T 
Sbjct: 69  ILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTA 128

Query: 122 DKSLISRLVPAMRTLHRALVLAGI-MDVKVTSPHSLGILSISEPPSMGRFRRGYDRAVFT 181
           D  LI  L+PAM++LH ALV A +   +++++PHSLG L+ S PPS  +FRRGYD  V  
Sbjct: 129 DHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLK 188

Query: 182 PMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATMDAIH 241
           P+L FLR T +PF+VNPYP+FGYS +  ++ALF+PN G++D+  K+ YTNM DA +D+++
Sbjct: 189 PLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSVY 248

Query: 242 TAMKAIGFADVDLVIGETGWPTNCD-GYVACSLENAATYNRNLIWHVNSGKGTPLMPNRR 301
           +AM  +GF+DV++VIGE GWP+  D   +   ++ AA +N+NLI  V+SG GTPLMPNR 
Sbjct: 249 SAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNRT 308

Query: 302 FETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDL----PNIMLGGRHGGRRTPTPVAP 361
           FETY+FALFNENLK GPT+ERN+G+F+ D TP+YD+    P           R+P     
Sbjct: 309 FETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDPVYNPRSPVR-GS 368

Query: 362 SGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQT 421
           S K+WCV ++ A   ALQ+NIDYVC   +DCRPI  GG C+ PN V++H+ Y MN YYQT
Sbjct: 369 SSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAMNLYYQT 428

Query: 422 NGRHDFNCDFGHTGVLTTVNPSHGGCAY 444
             +H+F+CDF +TG +TT++PS+G C Y
Sbjct: 429 MEKHEFDCDFDNTGEITTIDPSYGNCEY 455

[Close]

BLAST of Tan0013015 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 470.3 bits (1209), Expect = 1.7e-132
Identity = 227/442 (51.36%), Postives = 313/442 (70.81%), Query Frame = 0


Query: 2   SAAAASAVLLLLLQISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFDANPD 61
           S +  + V +LL+  + +  +IGVNYGTL NNL PP +V  F+   T I+R++LFD +P 
Sbjct: 9   SLSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQ 68

Query: 62  ILRAFAGTGILVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVGNEIMHTN 121
           IL+AFA TGI VTVTV N +IP LT+L++A+QW++ HI P  P T I  I VGNE++ T 
Sbjct: 69  ILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTA 128

Query: 122 DKSLISRLVPAMRTLHRALVLAGI-MDVKVTSPHSLGILSISEPPSMGRFRRGYDRAVFT 181
           D  LI  L+PAM++LH ALV A +   +++++PHSLG L+ S PPS  +FRRGYD  V  
Sbjct: 129 DHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLK 188

Query: 182 PMLQFLRETKAPFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATMDAIH 241
           P+L FLR T +PF+VNPYP+FGYS +  ++ALF+PN G++D+  K+ YTNM DA +D+++
Sbjct: 189 PLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSVY 248

Query: 242 TAMKAIGFADVDLVIGETGWPTNCD-GYVACSLENAATYNRNLIWHVNSGKGTPLMPNRR 301
           +AM  +GF+DV++VIGE GWP+  D   +   ++ AA +N+NLI  V+SG GTPLMPNR 
Sbjct: 249 SAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNRT 308

Query: 302 FETYLFALFNENLKPGPTAERNWGLFQPDFTPVYDL----PNIMLGGRHGGRRTPTPVAP 361
           FETY+FALFNENLK GPT+ERN+G+F+ D TP+YD+    P           R+P     
Sbjct: 309 FETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDPVYNPRSPVR-GS 368

Query: 362 SGKKWCVPRSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQT 421
           S K+WCV ++ A   ALQ+NIDYVC   +DCRPI  GG C+ PN V++H+ Y MN YYQT
Sbjct: 369 SSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAMNLYYQT 428

Query: 422 NGRHDFNCDFGHTGVLTTVNPS 438
             +H+F+CDF +TG +TT++PS
Sbjct: 429 MEKHEFDCDFDNTGEITTIDPS 449

[Close]

BLAST of Tan0013015 vs. TAIR 10
Match: AT2G39640.1 (glycosyl hydrolase family 17 protein )

HSP 1 Score: 443.0 bits (1138), Expect = 2.9e-124
Identity = 216/430 (50.23%), Postives = 293/430 (68.14%), Query Frame = 0


Query: 12  LLLQISTAVVAIGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFDANPDILRAFAGTGI 71
           ++ Q+S    AIG+NYGTLG NL+PP +VV+FI+TKT  D VK++DANPDILRAFAG+ I
Sbjct: 19  VIFQLSAVTSAIGINYGTLG-NLQPPQQVVDFIKTKTTFDSVKIYDANPDILRAFAGSEI 78

Query: 72  LVTVTVGNGEIPALTDLAAARQWVAAHIAPFHPQTRINYIAVGNEIMHTNDKSLISRLVP 131
            +T+ V NG IPA+ ++A ARQWVAA++ PF  Q +  Y+ VGNEI+ +ND +LIS LVP
Sbjct: 79  NITIMVPNGNIPAMVNVANARQWVAANVLPFQQQIKFKYVCVGNEILASNDNNLISNLVP 138

Query: 132 AMRTLHRALVLAGIMDVKVTSPHSLGILSISEPPSMGRFRRGYDRAVFTPMLQFLRETKA 191
           AM++L+ AL  + +  +KVT+PH+  I      PS  RF     + +FT +L+F R+ K+
Sbjct: 139 AMQSLNEALKASNLTYIKVTTPHAFTISYNRNTPSESRFTND-QKDIFTKILEFHRQAKS 198

Query: 192 PFMVNPYPYFGYSPKMANYALFQPNRGIYDKFNKITYTNMFDATMDAIHTAMKAIGFADV 251
           PFM+N Y +F       NYA+F P+  I D   + TYTNMFDA MDA ++AMKA+G+ DV
Sbjct: 199 PFMINAYTFFTMDTNNVNYAIFGPSNAITDTNTQQTYTNMFDAVMDATYSAMKALGYGDV 258

Query: 252 DLVIGETGWPTNCDGYVACSLENAATYNRNLIWHVNSGKGTPLMPNRRFETYLFALFNEN 311
           D+ +GETGWPT CD    CS +NA  YN N+I       GTPLMPNR  + ++FALFNE+
Sbjct: 259 DIAVGETGWPTACDA-SWCSPQNAENYNLNIIKRAQV-IGTPLMPNRHIDIFIFALFNED 318

Query: 312 LKPGPTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRRTPTPVAPSGKKWCVPRSDAAIEA 371
            KPGPT ERNWG+F+PDF+P+YD+  ++ G   GG   P P   +  KWCV + +A +  
Sbjct: 319 GKPGPTRERNWGIFKPDFSPMYDV-GVLKG---GGSPLPFPPINNNGKWCVGKPEATLMQ 378

Query: 372 LQKNIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQTNGRHDFNCDFGHTGVL 431
           LQ NID+VCS  IDC PI  GG CF  N++ + + ++MN+YYQ+ G  D  CDF  TG++
Sbjct: 379 LQANIDWVCSHGIDCTPISPGGICFDNNNMTTRSSFIMNAYYQSKGCVDVVCDFSGTGIV 438

Query: 432 TTVNPSHGGC 442
           T+ NPS   C
Sbjct: 439 TSTNPSTSTC 440

[Close]

BLAST of Tan0013015 vs. TAIR 10
Match: AT4G34480.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 385.6 bits (989), Expect = 5.5e-107
Identity = 191/429 (44.52%), Postives = 283/429 (65.97%), Query Frame = 0


Query: 23  IGVNYGTLGNNLRPPAEVVNFIRTKTKIDRVKLFDANPDILRAFAGTGILVTVTVGNGEI 82
           IGVNYG + +NL PP+E V  +++ T I +V+L+ A+P I++A AGTG+ + +   NG++
Sbjct: 26  IGVNYGQVADNLPPPSETVKLLQS-TSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDV 85

Query: 83  PAL-TDLAAARQWVAAHIAPFHPQTRINYIAVGNEIMHTNDKSLISRLVPAMRTLHRALV 142
           P+L +D  AA QW+ +++ PF+P ++I  I VGNEI+ +ND +L+++L+PAM+ + +AL 
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALE 145

Query: 143 LAGI-MDVKVTSPHSLGILSISEPPSMGRFRRGYDRAVFTPMLQFLRETKAPFMVNPYPY 202
              +   +KV++ +S+ +L  S+PPS G F  GY   +   +LQFL +T +PF +NPYP+
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGL-KGILQFLSDTGSPFAINPYPF 205

Query: 203 FGYS----PKMANYALFQPNRGIYDKFNKITYTNMFDATMDAIHTAMKAIGFADVDLVIG 262
           F Y     P+   + LF+PN G  D    I YTNMFDA +DA+H+A+K++GF  V++V+ 
Sbjct: 206 FAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVA 265

Query: 263 ETGWPTNCD-GYVACSLENAATYNRNLIWHVNSGKGTPLMPNRRFETYLFALFNENLKPG 322
           ETGW +  D   V  S++NA  YN NLI H+ S  GTPLMP +  +TY+FAL++ENLKPG
Sbjct: 266 ETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPG 325

Query: 323 PTAERNWGLFQPDFTPVYDLPNIMLGGRHGGRRTPT-PVAPSGKKWCVPRSDAAIEALQK 382
           P++ER +GLF+ D + VYD   + L       +TP+  V  SG  WCVP+  A  E LQ 
Sbjct: 326 PSSERAFGLFKTDLSMVYD---VGLAKSSSSSQTPSGKVTSSG--WCVPKKGATNEELQA 385

Query: 383 NIDYVCSSNIDCRPIQAGGACFQPNDVRSHAGYVMNSYYQTNGRHDFNCDFGHTGVLTTV 442
           ++D+ C   IDC  IQ GGACF+PN+V SHA Y MN Y+Q + +   +CDF  T  +T+ 
Sbjct: 386 SLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQ 445

Query: 443 NPSHGGCAY 444
           NPS+  C Y
Sbjct: 446 NPSYNNCVY 447

The following BLAST results are available for this feature:

BLAST of Tan0013015 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52409	6.5e-129	51.53	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1	[more]
Q9M069	7.8e-106	44.52	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]
Q94G86	4.0e-102	44.19	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]
Q8VYE5	4.7e-79	37.31	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q9FJU9	5.2e-78	37.81	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]

BLAST of Tan0013015 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891662.1	2.1e-233	87.69	glucan endo-1,3-beta-glucosidase, partial [Benincasa hispida]	[more]
XP_022992969.1	2.0e-228	84.89	glucan endo-1,3-beta-glucosidase [Cucurbita maxima]	[more]
XP_004147695.2	4.5e-228	85.91	glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KGN50054.2 hypothetical prot...	[more]
KAG6601002.1	5.9e-228	84.89	Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022957586.1	2.9e-227	84.67	glucan endo-1,3-beta-glucosidase-like [Cucurbita moschata]	[more]

BLAST of Tan0013015 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JYX6	9.8e-229	84.89	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111489139 PE=...	[more]
A0A6J1GZM3	1.4e-227	84.67	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111458933 P...	[more]
A0A5D3BZM0	5.0e-225	83.56	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3CF05	5.0e-225	83.56	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103500207 PE=3 SV...	[more]
A0A0A0KS71	2.5e-224	85.94	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G181480 PE=3...	[more]

BLAST of Tan0013015 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55430.1	5.6e-144	56.39	O-Glycosyl hydrolases family 17 protein	[more]
AT5G24318.1	4.8e-135	51.34	O-Glycosyl hydrolases family 17 protein	[more]
AT5G24318.2	1.7e-132	51.36	O-Glycosyl hydrolases family 17 protein	[more]
AT2G39640.1	2.9e-124	50.23	glycosyl hydrolase family 17 protein	[more]
AT4G34480.1	5.5e-107	44.52	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 21..338 e-value: 7.5E-117 score: 391.8
None	No IPR available	PANTHER	PTHR32227:SF371	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE-LIKE	coord: 9..335
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 23..335 e-value: 2.5E-83 score: 280.2
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 9..335
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 23..335

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0013015.1	Tan0013015.1	mRNA
Tan0013015.2	Tan0013015.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds