Tan0012971 (gene) Snake gourd v1

BLAST of Tan0012971 vs. ExPASy Swiss-Prot
Match: Q9SVG3 (Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=2 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.2e-167
Identity = 287/509 (56.39%), Postives = 375/509 (73.67%), Query Frame = 0

Query: 535  ASDQIPSHQTLLQCLSDHSSPSSSPISELVFFPDNPSYSPVLNSYIRNLRFASPTTPKPL 594
            +SD I  +++ +QC SD +    + I++ VF   NPS+S VL +YIRN RF + +TPKP 
Sbjct: 30   SSDSI--YESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRNGRFNTSSTPKPA 89

Query: 595  FIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDMFNLRSVAVDI 654
             IV P    H+ A + C +     ++IRSGGHDY+GLSY+SD PF ILDM NLR V+VDI
Sbjct: 90   IIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISDKPFFILDMSNLRDVSVDI 149

Query: 655  EDESAWVESGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVD 714
             D+SAW+ +GATLGEVY++I EKSK+HGFPAGVCPTVGVGGH+SG GYGN++RKFG+SVD
Sbjct: 150  ADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGYGNMLRKFGLSVD 209

Query: 715  NVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGVIVSWEFKLVPLPETVTVFRIEK 774
            N++DA IVDVNG++LDR+SMGEDLFWAI GGGGASFGV++ ++ KLVP+PETVTVFR+EK
Sbjct: 210  NLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVPVPETVTVFRVEK 269

Query: 775  TMEEGAVDILYKWQEVADQIDVNLFIRVVILPVNKKTQKTAKAKFISLFLGTAQELFALM 834
             M+ GAVD+++KWQ V  + D NLF+R++I PV +K  KT +A  ++LFLG A+E+ AL+
Sbjct: 270  YMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQPVTRKKVKTVRATVVALFLGRAEEVVALL 329

Query: 835  SENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTSLN--VLLERQPNSEKFLKKKSDYVQE 894
             + FPEL +  E+C EM+W  S L+W N    T ++  V L+R  +   F K+KSDYV  
Sbjct: 330  GKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNLDRANFGKRKSDYVAS 389

Query: 895  PISKANLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEE 954
             I +  +E + +KM EL +  L FNPYGGKM+++    TPFPHR+   +KIQYSVTW+E 
Sbjct: 390  EIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS-KLFKIQYSVTWQEN 449

Query: 955  GDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGNASYKQASTWGRKYF 1014
              E     L     LY +MT +VSK+PR+AYLNYRDVD+GVN +G  SY++   +GRKYF
Sbjct: 450  SVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDHGTNSYEEGEVYGRKYF 509

Query: 1015 KGNFDRLVKVKTMVDPDNFFRYEQSIPSV 1042
              NFDRLVKVKT  DPDNFFR EQSIP+V
Sbjct: 510  GDNFDRLVKVKTAADPDNFFRNEQSIPTV 535

BLAST of Tan0012971 vs. ExPASy Swiss-Prot
Match: F4HV09 (Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=3 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 4.3e-163
Identity = 288/514 (56.03%), Postives = 379/514 (73.74%), Query Frame = 0

Query: 4   SSSSILTFFSVILILSPWLSFAS---VQQSFLQCLITNSLPKFPISDAIFTPNNSSFLPI 63
           SS++    F+V L+L P  SFA+   ++ SF QC +T   P  PI +  +T  N +FL I
Sbjct: 3   SSTTQTLIFTVFLLLIP-TSFAAPPKLKDSFTQC-VTVFKPSVPIQNFTYTQQNPNFLTI 62

Query: 64  LNSYIRNLRFQTPTTPKPLVIVAAKHQSHVQSTVVCAKRVGLQIRIRSGGHDYEGLSYVS 123
           LN+Y+RNLR+   TT KP+ IVAA H +H+Q+T+ CAK++GLQ+RIRSGGHDY+G+SY+S
Sbjct: 63  LNNYVRNLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYLS 122

Query: 124 QQPFIILDLFNLRAIKVNIAKGTARVQAGATMGELYYAIANRS-NVHGFPGGICPTLGAG 183
              F++LD+FNLRAI+++    TA VQ+GAT+GE+YY +AN+S N+ GFP GICP LGAG
Sbjct: 123 TVDFVVLDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPGLGAG 182

Query: 184 GHLSGGGYGNLMRKFGLSIDNILDAQIVNVQGKIRNRQKMGEDLFWAIRGGGGGSFGVIL 243
           GH SGGGYGN+MRK+GLSIDNI+DA+IV+   ++ +R  MGEDLFWA+RGGG  SF V+L
Sbjct: 183 GHFSGGGYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCVVL 242

Query: 244 SWKIKLVPVPSTVTVFKVSRKIEEGA--TDVVWQWQQVMDKLDENLYIRLMLHSSKGKNG 303
           +WKIKLVPVP  VTVF V      G   TD+  +WQ++ DK+D +L+IRL L SS     
Sbjct: 243 AWKIKLVPVPEKVTVFNVETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTLSSS----- 302

Query: 304 QKTGKASLVALFLGPAEKLMEIVNKNIPSLKLQKQECIEMSWIESVLFWANFPNGTTPEA 363
            KT KAS + ++LG +EKL+EI+N   P L L K ECIEM WIESVLFW + P GT P +
Sbjct: 303 NKTVKASFMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTS 362

Query: 364 LLSRTIPSIP-FLKRKSDYVREPISREGIEAIWKAIMEIEEVGLVWNPYGGRMSQISETA 423
           ++   IP    +LKRKSDYV++PIS+ G+E+I+K + E E V + WNPYGGRMS+I  T 
Sbjct: 363 VMLNRIPQKQIYLKRKSDYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSEIPATE 422

Query: 424 TPFPHRAGVKFKIQYSANWKKGGDEETNENINLSRRLYEVMTPYVTKNPREAFLNYRDID 483
           T FPHRAG  FKIQYS+NW   G+E  ++ ++ + R++E M+PYV+KNPREAFLNYRDID
Sbjct: 423 TAFPHRAGNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDID 482

Query: 484 VGSTTHWSVEEGRVYGAKYFKGNFERLVDVKTRL 511
           +G   + + EEG+VYG KYFK NFERLV VKTR+
Sbjct: 483 IGKNLNSTYEEGKVYGVKYFKNNFERLVQVKTRV 509

BLAST of Tan0012971 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 5.6e-163
Identity = 283/524 (54.01%), Postives = 383/524 (73.09%), Query Frame = 0

Query: 522  ILTTLLLLLISMAASDQIPSHQTLLQCLSDHSSPSSSPISELVFFPDNPSYSPVLNSYIR 581
            ++  L  ++    +S    + +T  QCL+ +S P   PIS  +FF  N SYS VL + IR
Sbjct: 5    LILVLFFVVFIWQSSSSSANSETFTQCLTSNSDP-KHPISPAIFFSGNGSYSSVLQANIR 64

Query: 582  NLRFASPTTPKPLFIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLSYV--SDSPF 641
            NLRF + +TPKP  I+A TH SH+QA I C + H L+++IRSGGHDYDGLSYV  S  PF
Sbjct: 65   NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 124

Query: 642  VILDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSG 701
             +LDMFNLRSV VD+  ++AWV++GA LGEVY+ I EKSK   +PAG+CPTVGVGGH+SG
Sbjct: 125  FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 184

Query: 702  AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGVIVSWEFK 761
             GYGN+MRK+G++VDN +DA +VDVNG++LDR+ MGEDL+WAI GGGG S+GV+++++  
Sbjct: 185  GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 244

Query: 762  LVPLPETVTVFRIEKTMEEGAVDILYKWQEVADQIDVNLFIRVVILPVN--KKTQKTAKA 821
            LV +PE VTVFRI +T+E+ A DI+++WQ+VA ++   LFIR VI  VN    +QKT + 
Sbjct: 245  LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRT 304

Query: 822  KFISLFLGTAQELFALMSENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTSLNVLLERQP 881
             FI++FLG    L ++++  FPELG+   DC E SWI SVLFW+N  +G+S  +LL+R  
Sbjct: 305  TFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN- 364

Query: 882  NSEKFLKKKSDYVQEPISKANLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRA 941
                +LK+KSDYV+EPIS+  LE + +KMIEL+ P + FNPYGG+M +I  T TPFP+RA
Sbjct: 365  QPVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRA 424

Query: 942  GNKYKIQYSVTWKEEGDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNYRDVDLGVNG-N 1001
            GN +KIQY   W++E      + +E+ R+LY++MTP+VSK+PR ++ NYRDVDLG+N  N
Sbjct: 425  GNLWKIQYGANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHN 484

Query: 1002 GN-ASYKQASTWGRKYFKGNFDRLVKVKTMVDPDNFFRYEQSIP 1040
            G  +SY +   +G+KYF GNF+RLVK+KT VD  NFFR EQSIP
Sbjct: 485  GKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of Tan0012971 vs. ExPASy Swiss-Prot
Match: Q9SVG4 (Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 576.2 bits (1484), Expect = 7.3e-163
Identity = 284/519 (54.72%), Postives = 372/519 (71.68%), Query Frame = 0

Query: 526  LLLLLISMAASDQIPS-HQTLLQCLSDHSSPSSSPISELVFFPDNPSYSPVLNSYIRNLR 585
            LL L + +++  Q  S + + L+C SD +    S I++ VF   NP++S VL +YIRN R
Sbjct: 19   LLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNAR 78

Query: 586  FASPTTPKPLFIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDM 645
            F + +T KP  I+ P   SH+ A + C +     ++IRSGGHDYDGLSY+SD PF ILDM
Sbjct: 79   FNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDKPFFILDM 138

Query: 646  FNLRSVAVDIEDESAWVESGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGN 705
             N+R V+VDI   SAW+ +GATLGEVY++I EKS++HGFPAGVCPTVGVGGHLSG GYGN
Sbjct: 139  SNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGYGN 198

Query: 706  LMRKFGVSVDNVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGVIVSWEFKLVPLP 765
            ++RKFG+SVD V DA IVDVNGRVLDR++MGEDLFWAI GGGG S+GV++ ++ KLVP+P
Sbjct: 199  MVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPVP 258

Query: 766  ETVTVFRIEKTMEEGAVDILYKWQEVADQIDVNLFIRVVILPVNKKTQKTAKAKFISLFL 825
              VTVFR+E+ M+ GAVD+++KWQ V  + D NLF+R++I PV +K  KT +A  ++LFL
Sbjct: 259  SVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVTRKKVKTVRASVVALFL 318

Query: 826  GTAQELFALMSENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTSLN--VLLERQPNSEKF 885
            G A E+ AL+S+ FPELG+  E+C EM+W  S L+W N    T ++  V L+R  ++  F
Sbjct: 319  GRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLDTSSF 378

Query: 886  LKKKSDYVQEPISKANLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYK 945
             K+KSDYV   I K  +E + +KMIEL +  L FNPYGGKM+++     PFPHR    +K
Sbjct: 379  GKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-NKLFK 438

Query: 946  IQYSVTWKEEGDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGNASYK 1005
            IQYSV WKE   E     L   + LY +MT +VSK+PRS+Y NYRDVD+GVN +G  SYK
Sbjct: 439  IQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGANSYK 498

Query: 1006 QASTWGRKYFKGNFDRLVKVKTMVDPDNFFRYEQSIPSV 1042
            +   +GRKYF  NFDRLVK+KT VDP NFFR EQSIP++
Sbjct: 499  EGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTL 536

BLAST of Tan0012971 vs. ExPASy Swiss-Prot
Match: Q9LPC3 (Berberine bridge enzyme-like 1 OS=Arabidopsis thaliana OX=3702 GN=SEC1A PE=2 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.2e-159
Identity = 272/533 (51.03%), Postives = 386/533 (72.42%), Query Frame = 0

Query: 523  LTTLLLLLISMAASDQIPS-------HQTLLQCLSDHSSPSSSPISELVFFPDNPSYSPV 582
            L+ L+ L++S   S  + +       +++ LQC S+ +      + ++V    + S++P 
Sbjct: 3    LSCLVFLIVSSLVSSSLATAPPNTSIYESFLQCFSNQTGAPPEKLCDVVLPQSSASFTPT 62

Query: 583  LNSYIRNLRFASPTTPKPLFIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLSYVS 642
            L +YIRN RF + T+PKPL ++A     H+QAT+LC +    +++ RSGGHDYDG+SY+S
Sbjct: 63   LRAYIRNARFNTSTSPKPLLVIAARSECHVQATVLCTKSLNFQLKTRSGGHDYDGVSYIS 122

Query: 643  DSPFVILDMFNLRSVAVDIEDE--SAWVESGATLGEVYFKIAEKSK---IHGFPAGVCPT 702
            + PF +LDM  LR++ VD+ D+  SAWV +GATLGEVY+ I + SK    HGFPAGVCPT
Sbjct: 123  NRPFFVLDMSYLRNITVDMSDDGGSAWVGAGATLGEVYYNIWQSSKTHGTHGFPAGVCPT 182

Query: 703  VGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASF 762
            VG GGH+SG GYGN++RK+G+SVD V DA IVDVNGR+LDR+SMGEDLFWAI GGGGASF
Sbjct: 183  VGAGGHISGGGYGNMIRKYGLSVDYVTDAKIVDVNGRILDRKSMGEDLFWAIGGGGGASF 242

Query: 763  GVIVSWEFKLVPLPETVTVFRIEKTMEEGAVDILYKWQEVADQIDVNLFIRVVILPVNKK 822
            GVI+S++ KLVP+P  VTVFR+EKT+ E A+D+++KWQ VA +   +LF+R+++ PV + 
Sbjct: 243  GVILSFKIKLVPVPPRVTVFRVEKTLVENALDMVHKWQFVAPKTSPDLFMRLMLQPVTRN 302

Query: 823  TQKTAKAKFISLFLGTAQELFALMSENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTSL- 882
            T +T +A  ++LFLG   +L +L+++ FPELG+  E+C EM+WI SV++W+N    T + 
Sbjct: 303  TTQTVRASVVALFLGKQSDLMSLLTKEFPELGLKPENCTEMTWIQSVMWWANNDNATVIK 362

Query: 883  -NVLLERQPNSEKFLKKKSDYVQEPISKANLEGMMRKMIELKRPALTFNPYGGKMSQIPE 942
              +LL+R P+S  FLK+KSDYV++ ISK  L+ + +K++E  +  L FNPYGGKMS++  
Sbjct: 363  PEILLDRNPDSASFLKRKSDYVEKEISKDGLDFLCKKLMEAGKLGLVFNPYGGKMSEVAT 422

Query: 943  TETPFPHRAGNKYKIQYSVTWKEEGDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNYRD 1002
            T TPFPHR    +K+Q+S+ WK+ G +  +  +E  R  Y YM P+V+K+PR  YLNYRD
Sbjct: 423  TATPFPHRK-RLFKVQHSMNWKDPGTDVESSFMEKTRSFYSYMAPFVTKNPRHTYLNYRD 482

Query: 1003 VDLGVNGNGNASYKQASTWGRKYFKGNFDRLVKVKTMVDPDNFFRYEQSIPSV 1042
            +D+G+N +G  SY++A  +GRKYF  NFDRLVKVKT VDP+NFFR EQSIP++
Sbjct: 483  LDIGINSHGPNSYREAEVYGRKYFGENFDRLVKVKTAVDPENFFRDEQSIPTL 534

BLAST of Tan0012971 vs. NCBI nr
Match: QHO03835.1 (uncharacterized protein DS421_13g435560 [Arachis hypogaea])

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 561/1056 (53.12%), Postives = 772/1056 (73.11%), Query Frame = 0

Query: 3    SSSSSILTFFSVILILSPWLSF-------ASVQQSFLQCLITNSLPKFP-ISDAIFTPNN 62
            S+S   L+  S++L+L   +S         SV   FLQCL  ++    P +S  ++  +N
Sbjct: 4    STSLVHLSAISLLLVLVDHVSSLVAAPTPESVYTPFLQCLKNHTNSSSPQLSSILYARSN 63

Query: 63   SSFLPILNSYIRNLRFQTPTTPKPLVIVAAKHQSHVQSTVVCAKRVGLQIRIRSGGHDYE 122
            SSF  +L +Y+RN R+ + +TPKPL++V    QSHVQ  V+CAK +G+Q++IRSGGHD+E
Sbjct: 64   SSFSSVLQAYVRNARYNSTSTPKPLLVVTPLQQSHVQGAVICAKTIGVQLKIRSGGHDFE 123

Query: 123  GLSYVSQQPFIILDLFNLRAIKVNIAKGTARVQAGATMGELYYAIANRSNVHGFPGGICP 182
            G+SYVS +PFI+LD+FNLR I V++    A VQAGAT+GELYY I  +S V GFP G+CP
Sbjct: 124  GVSYVSHEPFIVLDMFNLRNITVDVQNEVAVVQAGATLGELYYRIWEKSKVLGFPSGVCP 183

Query: 183  TLGAGGHLSGGGYGNLMRKFGLSIDNILDAQIVNVQGKIRNRQKMGEDLFWAIRGGGGGS 242
            T+G GGH SGGGYGN++RK GLS+D++LDA+IV+V G++ N+++MG+DLFWAIRGGGG S
Sbjct: 184  TVGVGGHFSGGGYGNMIRKHGLSVDHVLDAEIVDVNGRVLNKEEMGDDLFWAIRGGGGSS 243

Query: 243  FGVILSWKIKLVPVPSTVTVFKVSRKIEEG--ATDVVWQWQQVMDKLDENLYIRLMLH-- 302
            FGVILS+ ++LV VP  VTVF+V R +E+    T++V QWQ+V    D+ L++RL+L   
Sbjct: 244  FGVILSYTVRLVSVPEIVTVFRVERSLEQNDTVTELVLQWQEVAPNTDDRLFMRLLLQPV 303

Query: 303  SSKGKNGQKTGKASLVALFLGPAEKLMEIVNKNIPSLKLQKQECIEMSWIESVLFWANFP 362
            SSK   G+KT +A+++ALFLG A++++ ++ K+ P L L+K+ C EMSWI+SV+ WANF 
Sbjct: 304  SSKAVKGKKTVRATVMALFLGGADEVVTLLGKDFPLLGLKKENCSEMSWIDSVICWANFD 363

Query: 363  NGTTPEALLSRTIPSIPFLKRKSDYVREPISREGIEAIWKAIMEIEEVGLVWNPYGGRMS 422
            NGT PEALL R   S  FLKRKSDYV+ PISR+G+E +WK ++E+ ++G V+NPYGG+M+
Sbjct: 364  NGTKPEALLGRNY-SAEFLKRKSDYVQTPISRDGLEGLWKEMIELGKIGFVFNPYGGKMN 423

Query: 423  QISETATPFPHRAGVKFKIQYSANWKKGGDEETNENINLSRRLYEVMTPYVTKNPREAFL 482
            +I   ATPFPHRAG  FKIQYS NW++    E    ++ + R+Y  MTP+V+ +PR AFL
Sbjct: 424  EILPDATPFPHRAGNLFKIQYSVNWEQSEVAEEKNFMDQATRVYSYMTPFVSNSPRRAFL 483

Query: 483  NYRDIDVGSTTHW--SVEEGRVYGAKYFKGNFERLVDVKTRLPMA--IPNSLLIPILTT- 542
            NYRD+D+G+      S EEG VYGAKYF  NF+RLV +KT +       N   IP   + 
Sbjct: 484  NYRDLDIGTNNFGKNSYEEGEVYGAKYFGNNFQRLVKIKTAVDPGNFFRNEQSIPTNPSG 543

Query: 543  LLLLLISMAASDQIPSHQTLLQCLSDHSSPSSSPISELVFFPDNPSYSPVLNSYIRNLRF 602
                  S+A S +     T L CL  H++   + +S +V+   N SY  VL +  RN RF
Sbjct: 544  GKPFNRSLAPSSRNSLFATFLNCLKTHTN-QPNQVSNIVYSQTNASYDSVLKASARNARF 603

Query: 603  ASPTTPKPLFIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDMF 662
            ++P+TPKPL I+ P + +H+Q ++LC +   ++++IRSGGHD++G+SYVS +PF+I+DMF
Sbjct: 604  SNPSTPKPLLIITPLNANHVQNSVLCAKSVGVQLKIRSGGHDFEGISYVSQAPFIIIDMF 663

Query: 663  NLRSVAVDIEDESAWVESGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNL 722
            + ++V+VD+++E A +E+GATLG+VY++I EKS +HGFPAGVCPTVGVGGHLSG GYGN+
Sbjct: 664  HFQNVSVDVQNEIAVIEAGATLGQVYYRIYEKSSVHGFPAGVCPTVGVGGHLSGGGYGNM 723

Query: 723  MRKFGVSVDNVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGVIVSWEFKLVPLPE 782
            +RK G+SVD+V+DA IVDVNGR+LD+E+MG+DLFWAIRGGGGASFGVI+S+  +LVP+PE
Sbjct: 724  IRKHGLSVDHVIDAKIVDVNGRILDKEAMGDDLFWAIRGGGGASFGVILSYTVRLVPVPE 783

Query: 783  TVTVFRIEKTMEEGAVDILYKWQEVADQIDVNLFIRVVILPVNKKTQKTAKAKFISLFLG 842
            TVTVF+++KT+E+ A D++++WQ+VA   D  LF+R+V+ PV K+T    +A  ++LFLG
Sbjct: 784  TVTVFQVDKTLEQNATDLVFQWQQVAPHTDERLFMRLVMQPVAKRTM---RASVMALFLG 843

Query: 843  TAQELFALMSENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTSLNVLLERQPNSEKFLKK 902
             A E+  L+ + FP LG+  E C EM WIDSVL+W+NY  G++L  LL+R      + KK
Sbjct: 844  GADEVVTLLEKEFPLLGLKKETCHEMRWIDSVLWWANYNDGSTLKALLDRSHYRVHYNKK 903

Query: 903  KSDYVQEPISKANLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQY 962
            KSDY+Q PISKA    + +KMIEL R  L FNPYGG+M+++P   TPFPHRAGN YK+QY
Sbjct: 904  KSDYIQTPISKAGFNLIWKKMIELGRVVLIFNPYGGRMNEVPYDATPFPHRAGNLYKVQY 963

Query: 963  SVTWKEEGDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGNASYKQAS 1022
            SVTW+E GD A  K +  IR +Y YMTP+VSK+PRSAY NYRD+D+GV  +G  SY+   
Sbjct: 964  SVTWEEGGDGADKKFIGEIRMMYNYMTPFVSKNPRSAYFNYRDLDIGVKMHGKDSYQDGV 1023

Query: 1023 TWGRKYFKGNFDRLVKVKTMVDPDNFFRYEQSIPSV 1042
             +G KYF  NF+RLVKVK+ +DP NFF  EQSIPS+
Sbjct: 1024 VYGSKYFNRNFERLVKVKSAIDPQNFFWNEQSIPSL 1054

BLAST of Tan0012971 vs. NCBI nr
Match: RYQ79210.1 (hypothetical protein Ahy_Scaffold6g107918 [Arachis hypogaea])

HSP 1 Score: 1117.1 bits (2888), Expect = 0.0e+00
Identity = 557/1085 (51.34%), Postives = 783/1085 (72.17%), Query Frame = 0

Query: 13   SVILILSPWL--SFASVQQSFLQCLITNSLPKFPISDAIFTPNNSSFLPILNSYIRNLRF 72
            +++L+LSP L  SF+S  ++F+ CL+ +S    PI+++IFTPNN SF  +L S IRNLRF
Sbjct: 7    TILLLLSPSLPFSFSSAHKTFIHCLVNHS-SSHPITESIFTPNNPSFSYVLQSNIRNLRF 66

Query: 73   QTPTTPKPLVIVAAKHQSHVQSTVVCAKRVGLQIRIRSGGHDYEGLSYVSQQPFIILDLF 132
             T TT KP +I+ A H SHVQ+ VVCA+R  + ++IRSGGHDYEG+SYV++ PF ILD+F
Sbjct: 67   NTSTTRKPFLIITALHASHVQAAVVCAQRHHMLMKIRSGGHDYEGVSYVAESPFFILDMF 126

Query: 133  NLRAIKVNIAKGTARVQAGATMGELYYAIANRSNVHGFPGGICPTLGAGGHLSGGGYGNL 192
            NLR+I V+    TA VQ+GAT+GELYY I  +S  HGFP G+CPT+G GGHL GGGYGN+
Sbjct: 127  NLRSIDVHTETETAWVQSGATLGELYYTIGEKSKTHGFPAGVCPTVGVGGHLGGGGYGNM 186

Query: 193  MRKFGLSIDNILDAQIVNVQGKIRNRQKMGEDLFWAIRGGGGGSFGVILSWKIKLVPVPS 252
            +RKFGL++D+++DAQ+V+VQG++ +R+ MGEDLFWAIRGGGG SF V++++KIKLV VP 
Sbjct: 187  LRKFGLAVDHVIDAQVVDVQGRLLDRESMGEDLFWAIRGGGGASFCVVIAYKIKLVRVPE 246

Query: 253  TVTVFKVSRKIEEGATDVVWQWQQVMDKLDENLYIRLMLH-SSKGKNGQKTGKASLVALF 312
             VTVF V + +E+ ATD+++ WQ V   +D NL+IRL++   +  +N  KT +A+ ++LF
Sbjct: 247  IVTVFHVKKTLEQNATDIIYTWQHVAPNIDNNLFIRLIMDVVNSTQNVTKTVRANFLSLF 306

Query: 313  LGPAEKLMEIVNKNIPSLKLQKQECIEMSWIES-VLFWANFPNGTTPEALLSRTIPSIPF 372
            LG ++ L+ ++N N P L L++Q+CIE +W++S V++W++    T  E LL R +PS  +
Sbjct: 307  LGDSKSLVSLMNVNFPQLGLKQQDCIETTWLQSTVIYWSDINPSTPLEVLLHRDLPS-DY 366

Query: 373  LKRKSDYVREPISREGIEAIWKAIMEIEEVGLVWNPYGGRMSQISETATPFPHRAGVKFK 432
             K KSDYV +PIS+ G+E IW+ ++E+++  + ++PYGGRM++I   ATPFP+R G  + 
Sbjct: 367  FKMKSDYVNKPISKVGLEGIWEKMIELKDTKVYFSPYGGRMNEIPSNATPFPYRGGTLWM 426

Query: 433  IQYSANWKKGGDEETNENINLSRRLYEVMTPYVTKNPREAFLNYRDIDVGSTTHW--SVE 492
            ++Y ANW + G +  +  +NL+R+L++ MTP+V+ NPR A+ NY+D D+G   +   S  
Sbjct: 427  VEYIANWFEPGKDVADYYMNLARKLHKHMTPFVSMNPRGAYYNYKDFDLGINHYGKSSYV 486

Query: 493  EGRVYGAKYFKGNFERLVDVKTRLPMA-----------IPNSLLI---------PI---- 552
            EG VYG +YF  NF RLV +KT +  A           +P  +++         PI    
Sbjct: 487  EGMVYGYQYFNDNFNRLVQIKTMVDPANFFRNEQSIPVVPYEMILDNGVQNKMEPIFSPK 546

Query: 553  -----LTTLLLLL---ISMAASDQIPSHQTLLQCLSDHSSPSSS--PISELVFFPDNPSY 612
                 L+TL+LL     S A       + T + CL + +S SSS  PI+E +F P+N S+
Sbjct: 547  PMLWFLSTLVLLSSLPCSSATYSAAEFNTTFIHCLINTTSDSSSSHPITESIFTPNNASF 606

Query: 613  SPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLS 672
            S VL SYIRNLRF + TT KPL I+   H+SH++A+ILC + H + ++IRSGGHDY+G+S
Sbjct: 607  SSVLQSYIRNLRFNTSTTKKPLLIITALHVSHVRASILCAQKHNMLMKIRSGGHDYEGVS 666

Query: 673  YVSDS--PFVILDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAEKSKIHGFPAGVCPT 732
            YV+ +  PF ILDMFNLRS+ +DIE E+AWV+ GATLGE+Y+ I  KSKIHGFPAGV P 
Sbjct: 667  YVTHNHVPFFILDMFNLRSIEIDIETETAWVQVGATLGELYYNIVHKSKIHGFPAGVGPN 726

Query: 733  VGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASF 792
            VGVGGHLSG GYGN+MRK+G+SVDNV+DA I+DV GR+LDR+SMGEDLFWAIRGGGGASF
Sbjct: 727  VGVGGHLSGGGYGNMMRKYGLSVDNVLDAQILDVQGRLLDRDSMGEDLFWAIRGGGGASF 786

Query: 793  GVIVSWEFKLVPLPETVTVFRIEKTMEEGAVDILYKWQEVADQIDVNLFIRVVILPVN-- 852
            GV++S++ KLV +PETVTVF++ +T+E+ A +++Y WQ  A   D NLFIR+++   N  
Sbjct: 787  GVVLSYKIKLVRVPETVTVFQVPRTLEQNATELVYNWQYFAPNTDPNLFIRLILEVANGA 846

Query: 853  KKTQKTAKAKFISLFLGTAQELFALMSENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTS 912
            +   KT +A F+++FLG ++ L +LM+E FP++G+  +DC E SW+ SVLFW N  I T+
Sbjct: 847  ETLTKTIRATFVAMFLGDSKSLVSLMNETFPQMGLRQQDCIETSWLKSVLFWDNMDISTT 906

Query: 913  -LNVLLERQPN-SEKFLKKKSDYVQEPISKANLEGMMRKMIELKRPALTFNPYGGKMSQI 972
             + +LLER P  + K LK+KSDYV++PISKA  EG+ +KMIEL+  A+ FNPYGG+M++I
Sbjct: 907  PVEILLERTPQYTLKHLKRKSDYVRKPISKAGWEGIWKKMIELENGAMYFNPYGGRMAEI 966

Query: 973  PETETPFPHRAGNKYKIQYSVTWKEEGDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNY 1032
            P   TP PHRAGN +KIQY   W ++G E   + + +IREL+ YM  +VSK+PR AY NY
Sbjct: 967  PSNATPIPHRAGNLWKIQYHANWNQKGKEVEDQYINMIRELHRYMAAFVSKNPRQAYFNY 1026

Query: 1033 RDVDLGVNGNGNASYKQASTWGRKYFKGNFDRLVKVKTMVDPDNFFRYEQSIPSVAFDSN 1052
            +D+DLG+N +G  SY Q   +G +YFK NF+RLV++KT VDP+NFFR EQS+P +++  +
Sbjct: 1027 KDLDLGINYHGKNSYYQGRVYGYEYFKDNFNRLVQIKTKVDPNNFFRNEQSVPVISYMGS 1086

BLAST of Tan0012971 vs. NCBI nr
Match: RYR12396.1 (hypothetical protein Ahy_B04g069936 [Arachis hypogaea])

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 555/1084 (51.20%), Postives = 781/1084 (72.05%), Query Frame = 0

Query: 13   SVILILSPWL--SFASVQQSFLQCLITN----SLPKFPISDAIFTPNNSSFLPILNSYIR 72
            +++L+LS  L  SF+S  ++F+ CL+ +    S    PI+++IFTPNN SF  +L S IR
Sbjct: 7    TILLLLSSSLPFSFSSAHKTFIHCLVNHSSSFSSSSHPITESIFTPNNPSFSYVLQSNIR 66

Query: 73   NLRFQTPTTPKPLVIVAAKHQSHVQSTVVCAKRVGLQIRIRSGGHDYEGLSYVSQQPFII 132
            NLRF T TT KP +I+ A H SHVQ+ VVCA+R  + ++IRSGGHDYEG+SYV++ PF I
Sbjct: 67   NLRFNTSTTRKPFLIITALHASHVQAAVVCAQRHHMLMKIRSGGHDYEGVSYVAESPFFI 126

Query: 133  LDLFNLRAIKVNIAKGTARVQAGATMGELYYAIANRSNVHGFPGGICPTLGAGGHLSGGG 192
            LD+FNLR+I V+I   TA VQ+GAT+GELYY I  +S  HGFP G+CPT+G GGH+ GGG
Sbjct: 127  LDMFNLRSIDVDIETETAWVQSGATLGELYYTIGEKSKTHGFPAGVCPTVGVGGHIGGGG 186

Query: 193  YGNLMRKFGLSIDNILDAQIVNVQGKIRNRQKMGEDLFWAIRGGGGGSFGVILSWKIKLV 252
            YGN++RKFGL++D+++DAQ+V+VQG++ +R+ MGEDLFWAIRGGGG SF V++++KIKLV
Sbjct: 187  YGNMLRKFGLAVDHVIDAQVVDVQGRLLDRESMGEDLFWAIRGGGGASFCVVIAYKIKLV 246

Query: 253  PVPSTVTVFKVSRKIEEGATDVVWQWQQVMDKLDENLYIRLMLH-SSKGKNGQKTGKASL 312
             VP  VTVF V + +E+ ATD+++ WQ V   +D NL+IRL++   +  +N  KT +A+ 
Sbjct: 247  RVPEIVTVFHVKKTLEQNATDIIYTWQHVAPNIDNNLFIRLIMDVVNSTQNVTKTVRANF 306

Query: 313  VALFLGPAEKLMEIVNKNIPSLKLQKQECIEMSWIES-VLFWANFPNGTTPEALLSRTIP 372
            ++LFLG ++ L+ ++N N P L L++Q+CIE +W++S V++W++    T  E LL R +P
Sbjct: 307  LSLFLGDSKSLVSLMNVNFPQLGLKQQDCIETTWLQSTVIYWSDINPSTPLEVLLHRDLP 366

Query: 373  SIPFLKRKSDYVREPISREGIEAIWKAIMEIEEVGLVWNPYGGRMSQISETATPFPHRAG 432
            S  + K KSDYV +PIS+ G+E IWK ++E+++  L + PYGGRM++I   ATPFP+R G
Sbjct: 367  S-DYFKMKSDYVNKPISKVGLEGIWKKMIELKDTKLYFTPYGGRMNEIPSNATPFPYRGG 426

Query: 433  VKFKIQYSANWKKGGDEETNENINLSRRLYEVMTPYVTKNPREAFLNYRDIDVGSTTHW- 492
              + ++Y ANW + G +  +  +NL+R+L++ MTP+V+ NPR A+ NY+D D+G   +  
Sbjct: 427  TLWMVEYIANWFEPGKDVADYYMNLARKLHKHMTPFVSMNPRGAYYNYKDFDLGINHYGK 486

Query: 493  -SVEEGRVYGAKYFKGNFERLVDVKTR---------------LPMAIPNSLLIPILT--- 552
             S  EG VYG +YF  NF RLV +KT                +P  +  + + PIL+   
Sbjct: 487  SSYVEGMVYGYQYFNDNFNRLVQIKTMVDPANFFRNEQSIPVVPYEMYKTKMEPILSPKP 546

Query: 553  -----TLLLLLISMAASDQIPS----HQTLLQCLSDHSSPSSS--PISELVFFPDNPSYS 612
                 + L+ L S+  S    S    + + + CL + +S SSS  PI+E +F P+N S+S
Sbjct: 547  MLWFLSTLVFLSSLPCSSATYSAAEFNTSFIHCLINTTSDSSSSHPITESIFTPNNASFS 606

Query: 613  PVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLSY 672
             VL SYIRNLRF + TT KPL I+   H+SH+QA+ILC + H + ++IRSGGHDY+G+SY
Sbjct: 607  SVLQSYIRNLRFNTSTTKKPLLIITALHVSHVQASILCAQKHNMLMKIRSGGHDYEGVSY 666

Query: 673  VSDS--PFVILDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAEKSKIHGFPAGVCPTV 732
            V+ +  PF ILDMFNLRS+ +DIE E+AWV+ GATLGE+Y+ I  KSKIHGFPAGV P V
Sbjct: 667  VTHNHVPFFILDMFNLRSIEIDIETETAWVQVGATLGELYYNIVHKSKIHGFPAGVGPNV 726

Query: 733  GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFG 792
            GVGGHLSG GYGN+MRK+G+SVDNV+DA I+DV GR+LDR+SMGEDLFWAIRGGGGASFG
Sbjct: 727  GVGGHLSGGGYGNMMRKYGLSVDNVLDAQILDVQGRLLDRDSMGEDLFWAIRGGGGASFG 786

Query: 793  VIVSWEFKLVPLPETVTVFRIEKTMEEGAVDILYKWQEVADQIDVNLFIRVVILPVN--K 852
            V++S++ KLV +PETVTVF++ +T+E+ A +++Y WQ  A   D NLFIR+++   N  +
Sbjct: 787  VVLSYKIKLVRVPETVTVFQVPRTLEQNATELVYNWQYFAPNTDPNLFIRLILEVANGAE 846

Query: 853  KTQKTAKAKFISLFLGTAQELFALMSENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTS- 912
               KT +A F+++FLG ++ L +LM+E FP++G+  +DC E +W+ SVLFW N  I T+ 
Sbjct: 847  TLTKTIRATFVAMFLGDSKSLVSLMNETFPQMGLRQQDCIETTWLKSVLFWDNMDISTTP 906

Query: 913  LNVLLERQPN-SEKFLKKKSDYVQEPISKANLEGMMRKMIELKRPALTFNPYGGKMSQIP 972
            + +LLER P  + K LK+KSDYV++PISKA  EG+ +KMIEL+  A+ FNPYGG+M++IP
Sbjct: 907  VEILLERTPQYTLKHLKRKSDYVRKPISKAGWEGIWKKMIELENGAMYFNPYGGRMAEIP 966

Query: 973  ETETPFPHRAGNKYKIQYSVTWKEEGDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNYR 1032
               TP PHRAGN +KIQY   W ++G E   + + +IREL+ YM  +VSK+PR AY NY+
Sbjct: 967  SNATPIPHRAGNLWKIQYHANWNQKGKEVEDQYINMIRELHRYMAAFVSKNPRQAYFNYK 1026

Query: 1033 DVDLGVNGNGNASYKQASTWGRKYFKGNFDRLVKVKTMVDPDNFFRYEQSIPSVAFDSNG 1052
            D+DLG+N +G  SY Q   +G +YFK NF+RLV++KT VDP+NFFR EQS+P +++  + 
Sbjct: 1027 DLDLGINYHGKNSYYQGRVYGYEYFKDNFNRLVQIKTKVDPNNFFRNEQSVPVISYMGSV 1086

BLAST of Tan0012971 vs. NCBI nr
Match: KAF4379527.1 (hypothetical protein G4B88_028769 [Cannabis sativa])

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 556/1037 (53.62%), Postives = 740/1037 (71.36%), Query Frame = 0

Query: 28   QQSFLQCLITNS-LPKFPISDAIFTPNNSSFLPILNSYIRNLRFQTPTTPKPLVIVAAKH 87
            Q+SFLQCL T S     PIS   F P++ S+  +LNSYIRNL F  PT+PKP  IVA  H
Sbjct: 9    QKSFLQCLSTYSPTSPLPISSVTFFPDDPSYPSVLNSYIRNLIFMLPTSPKPEFIVAPTH 68

Query: 88   QSHVQSTVVCAKRVGLQIRIRSGGHDYEGLSYVSQQPFIILDLFNLRAIKVNIAKGTARV 147
             SH+Q+ +VC K  GLQ+RIRSGGHDY+GLSYVS  PF+I+D+FNLR I V+    TA V
Sbjct: 69   VSHIQAAIVCCKIHGLQVRIRSGGHDYDGLSYVSNDPFVIIDMFNLRNITVSTEDETAWV 128

Query: 148  QAGATMGELYYAIANRSNVHGFPGGICPTLGAGGHLSGGGYGNLMRKFGLSIDNILDAQI 207
            ++GAT+GELYY IA +S +HGFP G+CPT+G GGH SGGGYGNLMRK GL++DNI+DA I
Sbjct: 129  ESGATIGELYYRIAEKSKIHGFPAGVCPTVGVGGHFSGGGYGNLMRKHGLTVDNIIDAVI 188

Query: 208  VNVQGKIRNRQKMGEDLFWAIRGGGGGSFGVILSWKIKLVPVPSTVTVFKVSRKIEEGAT 267
            V+V G+I NR  MGEDLFWAIRGGGG SFGVI+SWKIKLV VP  VTVF+V R +EEGAT
Sbjct: 189  VDVNGRILNRADMGEDLFWAIRGGGGASFGVIVSWKIKLVSVPEKVTVFRVVRTLEEGAT 248

Query: 268  DVVWQWQQVMDKLDENLYIRLMLHSSKGKNGQKTGKASLVALFLGPAEKLMEIVNKNIPS 327
            D+V QWQ V D+L   ++IR++L     K  QKT KA  VALFLG  E++  ++ K  P 
Sbjct: 249  DMVLQWQDVADQLLHEMFIRVVL-MPVNKKTQKTVKAKFVALFLGNKEQIGVLMEKKFPK 308

Query: 328  LKLQKQECIEMSWIESVLFWANFPNGTTPEALLSRTIPSIPFLKRKSDYVREPISREGIE 387
            L L+ ++ IEMSWIESVLFW+N+P GT+   LL R   S  FLK+KSDYV+EPI R+ +E
Sbjct: 309  LGLKPEDYIEMSWIESVLFWSNYPIGTSMNVLLERQPKSEKFLKKKSDYVQEPIQRDALE 368

Query: 388  AIWKAIMEIEEVGLVWNPYGGRMSQISETATPFPHRAGVKFKIQYSANWKKGGDEETNEN 447
             +WK ++E+++  L +NPYGG+MS+ISE  TPFPHRAG K+KIQYS  WK+   E  ++N
Sbjct: 369  GLWKKMIELKKPALTFNPYGGKMSEISELETPFPHRAGNKYKIQYSVQWKEEDSESLDQN 428

Query: 448  INLSRRLYEVMTPYVTKNPREAFLNYRDIDVGST---THWSVEEGRVYGAKYFKGNFER- 507
            I+  R+LY+ MTPYV+K+PR ++LNYRD+D+G+     + +  +   +G KYFKGNF+R 
Sbjct: 429  IDRIRKLYDHMTPYVSKSPRCSYLNYRDVDLGTNGVGYNANYSQASTWGTKYFKGNFDRW 488

Query: 508  LVDVKTRLPMAIP-----NSLLIPILTTLLLLLISMAASDQI-----PSHQT----LLQC 567
            L + +  + +A P       ++  I  TL+ +L+++  S  +     P++++     LQC
Sbjct: 489  LEEDEEGVGLACPPPNYHMEIIKMIPLTLIFVLLNIFYSSSLLAGSTPANESYYLPFLQC 548

Query: 568  LSDHSSPSSSPISELVFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQAT 627
            +   +S  S  + ++++   NPSY+ VL +YIRN RF + +T KP  I+ P   S + A 
Sbjct: 549  M--QNSTKSIKVKDIIYNEANPSYTSVLRAYIRNSRFNTSSTLKPSLIITPATESDVAAA 608

Query: 628  ILCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDMFNLRSVAVDIEDESAWVESGATLG 687
            +LC +  +L ++ RSGGHDY G+SY S  PFVILDMF LRSV VD+ D+SAWV++GATLG
Sbjct: 609  VLCTKATSLRLKTRSGGHDYAGVSYTSTVPFVILDMFQLRSVTVDVADQSAWVQAGATLG 668

Query: 688  EVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRV 747
            E+Y+ I  KSK+ GFPAG+CPTVG GGH+SG GYGN++RK+G++ D V+DA IVD  G +
Sbjct: 669  ELYYNIWNKSKVLGFPAGICPTVGAGGHISGGGYGNMIRKYGLASDYVIDAKIVDAKGNI 728

Query: 748  LDRESMGEDLFWAIRGGGGASFGVIVSWEFKLVPLPETVTVFRIEKTME---EGAVDILY 807
            LDR+ MGEDLFWAIRGGGGASFGVI+S++ KLV +PETVTVF  E+++E   +   D + 
Sbjct: 729  LDRKQMGEDLFWAIRGGGGASFGVILSYKIKLVQVPETVTVFNAERSLENKNDSMTDFVV 788

Query: 808  KWQEVADQIDVNLFIRVVILPVNK---KTQKTAKAKFISLFLGTAQELFALMSENFPELG 867
            +WQ+VA   D  LF+R+++ P      K +KT K   ++ +LG A  L ++M + FP LG
Sbjct: 789  QWQKVAPTTDEGLFMRMLVQPSTSTVTKGEKTMKVTIVAQYLGNADTLISVMGKEFPLLG 848

Query: 868  VTAEDCKEMSWIDSVLFWSNYPIGTSL-NVLLERQPNSEKFLKKKSDYVQEPISKANLEG 927
            +  EDCKE+SWI+SVL+W      T+   VLL+R  NS  F  +KSDYVQ+ IS   LE 
Sbjct: 849  LKKEDCKELSWINSVLWWLKLKNDTATPEVLLDRNVNSAIFGIRKSDYVQKEISATVLES 908

Query: 928  MMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAAAKNL 987
            + +KM   K   L FNPYGGKM+++P TETPFPHRAGN +K+QYSV WKE+  +A +K L
Sbjct: 909  IWKKMTPGK-VGLVFNPYGGKMNKVPSTETPFPHRAGNLFKVQYSVGWKEDEAQAESKYL 968

Query: 988  EVIRELYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGNASYKQASTWGRKYFKGNFDRLVK 1039
            E IR LY +MTPYVS++PRSA+LNYRD+D+G+N  G  SY++   +G KYF  NFDRLVK
Sbjct: 969  EEIRSLYSFMTPYVSQNPRSAFLNYRDLDIGINNFGKNSYEEGKVYGVKYFGNNFDRLVK 1028

BLAST of Tan0012971 vs. NCBI nr
Match: KAG5378460.1 (hypothetical protein IGI04_026302 [Brassica rapa subsp. trilocularis])

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 544/1053 (51.66%), Postives = 743/1053 (70.56%), Query Frame = 0

Query: 5    SSSILTFFSVILILSPWLSFAS---VQQSFLQCL--ITNSLPKFPISDAIFTPNNSSFLP 64
            S +I T    I   +   SFAS   ++  F QC      S P FPI +  +T  + SFL 
Sbjct: 3    SLTIHTLIITIFFFTISTSFASPPRLEDVFSQCTTDFKPSNPNFPIQNFTYTQQDPSFLT 62

Query: 65   ILNSYIRNLRFQTPTTPKPLVIVAAKHQSHVQSTVVCAKRVGLQIRIRSGGHDYEGLSYV 124
            ILN+Y+RNLR+   TT KP+ IVAA   +H+Q+T+ CAKR+ LQ+R+RSGGHDY+G+SY+
Sbjct: 63   ILNNYVRNLRYFNNTTRKPVAIVAAADVTHIQATITCAKRLSLQLRVRSGGHDYDGMSYL 122

Query: 125  SQQPFIILDLFNLRAIKVNIAKGTARVQAGATMGELYYAIANRSN-VHGFPGGICPTLGA 184
            S   F++LD+FNLR+I+ +    TA VQ+GAT+GE+YY +AN+SN + GFP GICP LGA
Sbjct: 123  STVEFVVLDMFNLRSIEFDPKLDTAWVQSGATLGEIYYGVANKSNDLRGFPAGICPGLGA 182

Query: 185  GGHLSGGGYGNLMRKFGLSIDNILDAQIVNVQGKIRNRQKMGEDLFWAIRGGGGGSFGVI 244
            GGH SGGGYGN+MRK+GLSIDNI+DA+IV+ +G++ +R  MGEDLFWA+RGGG  SF V+
Sbjct: 183  GGHFSGGGYGNMMRKYGLSIDNIIDAKIVDAKGRVLDRSSMGEDLFWALRGGGAASFCVV 242

Query: 245  LSWKIKLVPVPSTVTVFKVSRKIEEG---ATDVVWQWQQVMDKLDENLYIRLMLHSSKGK 304
            L+WKIKLVPVP  VTVF V      G    T++V +WQ++ DK D NL+IRL L +S   
Sbjct: 243  LAWKIKLVPVPGKVTVFNVETVGNRGGVNTTELVLKWQEIADKTDNNLFIRLTLSTS--- 302

Query: 305  NGQKTGKASLVALFLGPAEKLMEIVNKNIPSLKLQKQECIEMSWIESVLFWANFPNGTTP 364
               KT KAS + +FLG + KL+EI+N + P L L K EC+EM WIES LFW + P GT P
Sbjct: 303  --NKTVKASFMGMFLGDSTKLLEIMNTDFPELGLNKSECLEMKWIESALFWLSIPLGTAP 362

Query: 365  EALLSRTIPSIP-FLKRKSDYVREPISREGIEAIWKAIMEIEEVGLVWNPYGGRMSQISE 424
             +++   IP    +LKRKSDYV++PI R G++AI+K ++E E V + WNPYGGRMS+I  
Sbjct: 363  TSVILNRIPQKQIYLKRKSDYVQKPIPRTGLDAIFKVLLENENVTMAWNPYGGRMSEIPS 422

Query: 425  TATPFPHRAGVKFKIQYSANWKKGGDEETNENINLSRRLYEVMTPYVTKNPREAFLNYRD 484
            T T FPHR G  FKIQY+ANW   G+   NE ++ + R++E M+PYV+KNPREAFLNYRD
Sbjct: 423  TETAFPHRPGNMFKIQYAANWFVQGEAVANECLSQTERVFEAMSPYVSKNPREAFLNYRD 482

Query: 485  IDVGSTTHWSVEEGRVYGAKYFKGNFERLVDVKTRLPMAIPNSLL-----IPILTTLLLL 544
            +D+G++ + + EEG+VYG KYFK NF+RLV VK+R+    P++       IP+L      
Sbjct: 483  VDIGTSFNSTYEEGKVYGVKYFKNNFDRLVKVKSRVD---PDNFFRYEQSIPVL------ 542

Query: 545  LISMAASDQIPSHQTLLQCLSDHSSPSSSPISELVFFPDNPSYSPVLNSYIRNLRFASPT 604
              S+A S  +      L+CL    +  +SP S + + P N S++ VL S I NLRF  PT
Sbjct: 543  --SIATSQPVTDPVAFLRCLERQPTDPASPNSAVAYIPTNSSFTTVLRSRIPNLRFDKPT 602

Query: 605  TPKPLFIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDMFNLRS 664
            TPKPL IVA    +HIQA + C R  +L++RIRSGGHD++GLSY S  PF +LDMF+ R+
Sbjct: 603  TPKPLAIVAAATWTHIQAALGCARELSLQVRIRSGGHDFEGLSYTSTVPFFVLDMFSFRT 662

Query: 665  VAVDIEDESAWVESGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKF 724
            V V++ D +AWV++G T GE+Y++IAEKS + GFPAG+C T+GVGGH SG GYG +MRK+
Sbjct: 663  VDVNLTDGTAWVDAGVTTGELYYRIAEKSNVLGFPAGLCTTLGVGGHFSGGGYGTMMRKY 722

Query: 725  GVSVDNVVDALIVDVNGRVL-DRESMGEDLFWAIRGGGGASFGVIVSWEFKLVPLPETVT 784
            G+SVDNVV + IVD NG +  DR SMGED FWAIRGGG ASFGV++ ++ +LVP+PE VT
Sbjct: 723  GLSVDNVVGSGIVDSNGNIFTDRVSMGEDRFWAIRGGGAASFGVVLGYKIRLVPVPEKVT 782

Query: 785  VFRIEKTMEEGAVDILYKWQEVADQIDVNLFIRVVILPVN--KKTQKTAKAKFISLFLGT 844
            VF + +T+ EGAVD++ KWQ  A   D NLF+R+ +  VN  K  +K   A FI ++LG 
Sbjct: 783  VFNVGRTVAEGAVDLIMKWQSFAHSTDRNLFVRLTLTLVNGTKPGEKMVLASFIGMYLGG 842

Query: 845  AQELFALMSENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTSLNVLLERQPNSEKFLKKK 904
            + + F +M+ +FPEL +   DC EM WI+SV+FW+ +P GT ++VLL R   ++ F K+K
Sbjct: 843  SDKTFNVMNRDFPELKLKRTDCTEMRWIESVVFWAGFPTGTPISVLLNRTVTNKAFHKRK 902

Query: 905  SDYVQEPISKANLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYS 964
            SDYV+ PIS+  L  +++K++E++   + FNPYGG+M +IP + TPFPHRAGN YKI+Y 
Sbjct: 903  SDYVKRPISRIGLGLILKKLVEVENVRMFFNPYGGRMGEIPSSRTPFPHRAGNLYKIEYL 962

Query: 965  VTWKEEGDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGNASYKQAST 1024
            V+W E G++     L   RE+YE+M+PYVSK+PR A+ NYRD+D+G   + N++Y++   
Sbjct: 963  VSWSEAGEDVEKNQLARAREMYEFMSPYVSKNPREAFFNYRDLDIG--SSVNSTYEEGKV 1022

Query: 1025 WGRKYFKGNFDRLVKVKTMVDPDNFFRYEQSIP 1040
            +G KYFK NF+RLV +KT++D +NF++ EQ IP
Sbjct: 1023 YGVKYFKDNFERLVDIKTLIDAENFWKNEQGIP 1037

BLAST of Tan0012971 vs. ExPASy TrEMBL
Match: A0A444WP58 (Uncharacterized protein OS=Arachis hypogaea OX=3818 GN=Ahy_Scaffold6g107918 PE=3 SV=1)

HSP 1 Score: 1117.1 bits (2888), Expect = 0.0e+00
Identity = 557/1085 (51.34%), Postives = 783/1085 (72.17%), Query Frame = 0

Query: 13   SVILILSPWL--SFASVQQSFLQCLITNSLPKFPISDAIFTPNNSSFLPILNSYIRNLRF 72
            +++L+LSP L  SF+S  ++F+ CL+ +S    PI+++IFTPNN SF  +L S IRNLRF
Sbjct: 7    TILLLLSPSLPFSFSSAHKTFIHCLVNHS-SSHPITESIFTPNNPSFSYVLQSNIRNLRF 66

Query: 73   QTPTTPKPLVIVAAKHQSHVQSTVVCAKRVGLQIRIRSGGHDYEGLSYVSQQPFIILDLF 132
             T TT KP +I+ A H SHVQ+ VVCA+R  + ++IRSGGHDYEG+SYV++ PF ILD+F
Sbjct: 67   NTSTTRKPFLIITALHASHVQAAVVCAQRHHMLMKIRSGGHDYEGVSYVAESPFFILDMF 126

Query: 133  NLRAIKVNIAKGTARVQAGATMGELYYAIANRSNVHGFPGGICPTLGAGGHLSGGGYGNL 192
            NLR+I V+    TA VQ+GAT+GELYY I  +S  HGFP G+CPT+G GGHL GGGYGN+
Sbjct: 127  NLRSIDVHTETETAWVQSGATLGELYYTIGEKSKTHGFPAGVCPTVGVGGHLGGGGYGNM 186

Query: 193  MRKFGLSIDNILDAQIVNVQGKIRNRQKMGEDLFWAIRGGGGGSFGVILSWKIKLVPVPS 252
            +RKFGL++D+++DAQ+V+VQG++ +R+ MGEDLFWAIRGGGG SF V++++KIKLV VP 
Sbjct: 187  LRKFGLAVDHVIDAQVVDVQGRLLDRESMGEDLFWAIRGGGGASFCVVIAYKIKLVRVPE 246

Query: 253  TVTVFKVSRKIEEGATDVVWQWQQVMDKLDENLYIRLMLH-SSKGKNGQKTGKASLVALF 312
             VTVF V + +E+ ATD+++ WQ V   +D NL+IRL++   +  +N  KT +A+ ++LF
Sbjct: 247  IVTVFHVKKTLEQNATDIIYTWQHVAPNIDNNLFIRLIMDVVNSTQNVTKTVRANFLSLF 306

Query: 313  LGPAEKLMEIVNKNIPSLKLQKQECIEMSWIES-VLFWANFPNGTTPEALLSRTIPSIPF 372
            LG ++ L+ ++N N P L L++Q+CIE +W++S V++W++    T  E LL R +PS  +
Sbjct: 307  LGDSKSLVSLMNVNFPQLGLKQQDCIETTWLQSTVIYWSDINPSTPLEVLLHRDLPS-DY 366

Query: 373  LKRKSDYVREPISREGIEAIWKAIMEIEEVGLVWNPYGGRMSQISETATPFPHRAGVKFK 432
             K KSDYV +PIS+ G+E IW+ ++E+++  + ++PYGGRM++I   ATPFP+R G  + 
Sbjct: 367  FKMKSDYVNKPISKVGLEGIWEKMIELKDTKVYFSPYGGRMNEIPSNATPFPYRGGTLWM 426

Query: 433  IQYSANWKKGGDEETNENINLSRRLYEVMTPYVTKNPREAFLNYRDIDVGSTTHW--SVE 492
            ++Y ANW + G +  +  +NL+R+L++ MTP+V+ NPR A+ NY+D D+G   +   S  
Sbjct: 427  VEYIANWFEPGKDVADYYMNLARKLHKHMTPFVSMNPRGAYYNYKDFDLGINHYGKSSYV 486

Query: 493  EGRVYGAKYFKGNFERLVDVKTRLPMA-----------IPNSLLI---------PI---- 552
            EG VYG +YF  NF RLV +KT +  A           +P  +++         PI    
Sbjct: 487  EGMVYGYQYFNDNFNRLVQIKTMVDPANFFRNEQSIPVVPYEMILDNGVQNKMEPIFSPK 546

Query: 553  -----LTTLLLLL---ISMAASDQIPSHQTLLQCLSDHSSPSSS--PISELVFFPDNPSY 612
                 L+TL+LL     S A       + T + CL + +S SSS  PI+E +F P+N S+
Sbjct: 547  PMLWFLSTLVLLSSLPCSSATYSAAEFNTTFIHCLINTTSDSSSSHPITESIFTPNNASF 606

Query: 613  SPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLS 672
            S VL SYIRNLRF + TT KPL I+   H+SH++A+ILC + H + ++IRSGGHDY+G+S
Sbjct: 607  SSVLQSYIRNLRFNTSTTKKPLLIITALHVSHVRASILCAQKHNMLMKIRSGGHDYEGVS 666

Query: 673  YVSDS--PFVILDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAEKSKIHGFPAGVCPT 732
            YV+ +  PF ILDMFNLRS+ +DIE E+AWV+ GATLGE+Y+ I  KSKIHGFPAGV P 
Sbjct: 667  YVTHNHVPFFILDMFNLRSIEIDIETETAWVQVGATLGELYYNIVHKSKIHGFPAGVGPN 726

Query: 733  VGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASF 792
            VGVGGHLSG GYGN+MRK+G+SVDNV+DA I+DV GR+LDR+SMGEDLFWAIRGGGGASF
Sbjct: 727  VGVGGHLSGGGYGNMMRKYGLSVDNVLDAQILDVQGRLLDRDSMGEDLFWAIRGGGGASF 786

Query: 793  GVIVSWEFKLVPLPETVTVFRIEKTMEEGAVDILYKWQEVADQIDVNLFIRVVILPVN-- 852
            GV++S++ KLV +PETVTVF++ +T+E+ A +++Y WQ  A   D NLFIR+++   N  
Sbjct: 787  GVVLSYKIKLVRVPETVTVFQVPRTLEQNATELVYNWQYFAPNTDPNLFIRLILEVANGA 846

Query: 853  KKTQKTAKAKFISLFLGTAQELFALMSENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTS 912
            +   KT +A F+++FLG ++ L +LM+E FP++G+  +DC E SW+ SVLFW N  I T+
Sbjct: 847  ETLTKTIRATFVAMFLGDSKSLVSLMNETFPQMGLRQQDCIETSWLKSVLFWDNMDISTT 906

Query: 913  -LNVLLERQPN-SEKFLKKKSDYVQEPISKANLEGMMRKMIELKRPALTFNPYGGKMSQI 972
             + +LLER P  + K LK+KSDYV++PISKA  EG+ +KMIEL+  A+ FNPYGG+M++I
Sbjct: 907  PVEILLERTPQYTLKHLKRKSDYVRKPISKAGWEGIWKKMIELENGAMYFNPYGGRMAEI 966

Query: 973  PETETPFPHRAGNKYKIQYSVTWKEEGDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNY 1032
            P   TP PHRAGN +KIQY   W ++G E   + + +IREL+ YM  +VSK+PR AY NY
Sbjct: 967  PSNATPIPHRAGNLWKIQYHANWNQKGKEVEDQYINMIRELHRYMAAFVSKNPRQAYFNY 1026

Query: 1033 RDVDLGVNGNGNASYKQASTWGRKYFKGNFDRLVKVKTMVDPDNFFRYEQSIPSVAFDSN 1052
            +D+DLG+N +G  SY Q   +G +YFK NF+RLV++KT VDP+NFFR EQS+P +++  +
Sbjct: 1027 KDLDLGINYHGKNSYYQGRVYGYEYFKDNFNRLVQIKTKVDPNNFFRNEQSVPVISYMGS 1086

BLAST of Tan0012971 vs. ExPASy TrEMBL
Match: A0A444ZDX7 (Uncharacterized protein OS=Arachis hypogaea OX=3818 GN=Ahy_B04g069936 PE=3 SV=1)

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 555/1084 (51.20%), Postives = 781/1084 (72.05%), Query Frame = 0

Query: 13   SVILILSPWL--SFASVQQSFLQCLITN----SLPKFPISDAIFTPNNSSFLPILNSYIR 72
            +++L+LS  L  SF+S  ++F+ CL+ +    S    PI+++IFTPNN SF  +L S IR
Sbjct: 7    TILLLLSSSLPFSFSSAHKTFIHCLVNHSSSFSSSSHPITESIFTPNNPSFSYVLQSNIR 66

Query: 73   NLRFQTPTTPKPLVIVAAKHQSHVQSTVVCAKRVGLQIRIRSGGHDYEGLSYVSQQPFII 132
            NLRF T TT KP +I+ A H SHVQ+ VVCA+R  + ++IRSGGHDYEG+SYV++ PF I
Sbjct: 67   NLRFNTSTTRKPFLIITALHASHVQAAVVCAQRHHMLMKIRSGGHDYEGVSYVAESPFFI 126

Query: 133  LDLFNLRAIKVNIAKGTARVQAGATMGELYYAIANRSNVHGFPGGICPTLGAGGHLSGGG 192
            LD+FNLR+I V+I   TA VQ+GAT+GELYY I  +S  HGFP G+CPT+G GGH+ GGG
Sbjct: 127  LDMFNLRSIDVDIETETAWVQSGATLGELYYTIGEKSKTHGFPAGVCPTVGVGGHIGGGG 186

Query: 193  YGNLMRKFGLSIDNILDAQIVNVQGKIRNRQKMGEDLFWAIRGGGGGSFGVILSWKIKLV 252
            YGN++RKFGL++D+++DAQ+V+VQG++ +R+ MGEDLFWAIRGGGG SF V++++KIKLV
Sbjct: 187  YGNMLRKFGLAVDHVIDAQVVDVQGRLLDRESMGEDLFWAIRGGGGASFCVVIAYKIKLV 246

Query: 253  PVPSTVTVFKVSRKIEEGATDVVWQWQQVMDKLDENLYIRLMLH-SSKGKNGQKTGKASL 312
             VP  VTVF V + +E+ ATD+++ WQ V   +D NL+IRL++   +  +N  KT +A+ 
Sbjct: 247  RVPEIVTVFHVKKTLEQNATDIIYTWQHVAPNIDNNLFIRLIMDVVNSTQNVTKTVRANF 306

Query: 313  VALFLGPAEKLMEIVNKNIPSLKLQKQECIEMSWIES-VLFWANFPNGTTPEALLSRTIP 372
            ++LFLG ++ L+ ++N N P L L++Q+CIE +W++S V++W++    T  E LL R +P
Sbjct: 307  LSLFLGDSKSLVSLMNVNFPQLGLKQQDCIETTWLQSTVIYWSDINPSTPLEVLLHRDLP 366

Query: 373  SIPFLKRKSDYVREPISREGIEAIWKAIMEIEEVGLVWNPYGGRMSQISETATPFPHRAG 432
            S  + K KSDYV +PIS+ G+E IWK ++E+++  L + PYGGRM++I   ATPFP+R G
Sbjct: 367  S-DYFKMKSDYVNKPISKVGLEGIWKKMIELKDTKLYFTPYGGRMNEIPSNATPFPYRGG 426

Query: 433  VKFKIQYSANWKKGGDEETNENINLSRRLYEVMTPYVTKNPREAFLNYRDIDVGSTTHW- 492
              + ++Y ANW + G +  +  +NL+R+L++ MTP+V+ NPR A+ NY+D D+G   +  
Sbjct: 427  TLWMVEYIANWFEPGKDVADYYMNLARKLHKHMTPFVSMNPRGAYYNYKDFDLGINHYGK 486

Query: 493  -SVEEGRVYGAKYFKGNFERLVDVKTR---------------LPMAIPNSLLIPILT--- 552
             S  EG VYG +YF  NF RLV +KT                +P  +  + + PIL+   
Sbjct: 487  SSYVEGMVYGYQYFNDNFNRLVQIKTMVDPANFFRNEQSIPVVPYEMYKTKMEPILSPKP 546

Query: 553  -----TLLLLLISMAASDQIPS----HQTLLQCLSDHSSPSSS--PISELVFFPDNPSYS 612
                 + L+ L S+  S    S    + + + CL + +S SSS  PI+E +F P+N S+S
Sbjct: 547  MLWFLSTLVFLSSLPCSSATYSAAEFNTSFIHCLINTTSDSSSSHPITESIFTPNNASFS 606

Query: 613  PVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLSY 672
             VL SYIRNLRF + TT KPL I+   H+SH+QA+ILC + H + ++IRSGGHDY+G+SY
Sbjct: 607  SVLQSYIRNLRFNTSTTKKPLLIITALHVSHVQASILCAQKHNMLMKIRSGGHDYEGVSY 666

Query: 673  VSDS--PFVILDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAEKSKIHGFPAGVCPTV 732
            V+ +  PF ILDMFNLRS+ +DIE E+AWV+ GATLGE+Y+ I  KSKIHGFPAGV P V
Sbjct: 667  VTHNHVPFFILDMFNLRSIEIDIETETAWVQVGATLGELYYNIVHKSKIHGFPAGVGPNV 726

Query: 733  GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFG 792
            GVGGHLSG GYGN+MRK+G+SVDNV+DA I+DV GR+LDR+SMGEDLFWAIRGGGGASFG
Sbjct: 727  GVGGHLSGGGYGNMMRKYGLSVDNVLDAQILDVQGRLLDRDSMGEDLFWAIRGGGGASFG 786

Query: 793  VIVSWEFKLVPLPETVTVFRIEKTMEEGAVDILYKWQEVADQIDVNLFIRVVILPVN--K 852
            V++S++ KLV +PETVTVF++ +T+E+ A +++Y WQ  A   D NLFIR+++   N  +
Sbjct: 787  VVLSYKIKLVRVPETVTVFQVPRTLEQNATELVYNWQYFAPNTDPNLFIRLILEVANGAE 846

Query: 853  KTQKTAKAKFISLFLGTAQELFALMSENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTS- 912
               KT +A F+++FLG ++ L +LM+E FP++G+  +DC E +W+ SVLFW N  I T+ 
Sbjct: 847  TLTKTIRATFVAMFLGDSKSLVSLMNETFPQMGLRQQDCIETTWLKSVLFWDNMDISTTP 906

Query: 913  LNVLLERQPN-SEKFLKKKSDYVQEPISKANLEGMMRKMIELKRPALTFNPYGGKMSQIP 972
            + +LLER P  + K LK+KSDYV++PISKA  EG+ +KMIEL+  A+ FNPYGG+M++IP
Sbjct: 907  VEILLERTPQYTLKHLKRKSDYVRKPISKAGWEGIWKKMIELENGAMYFNPYGGRMAEIP 966

Query: 973  ETETPFPHRAGNKYKIQYSVTWKEEGDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNYR 1032
               TP PHRAGN +KIQY   W ++G E   + + +IREL+ YM  +VSK+PR AY NY+
Sbjct: 967  SNATPIPHRAGNLWKIQYHANWNQKGKEVEDQYINMIRELHRYMAAFVSKNPRQAYFNYK 1026

Query: 1033 DVDLGVNGNGNASYKQASTWGRKYFKGNFDRLVKVKTMVDPDNFFRYEQSIPSVAFDSNG 1052
            D+DLG+N +G  SY Q   +G +YFK NF+RLV++KT VDP+NFFR EQS+P +++  + 
Sbjct: 1027 DLDLGINYHGKNSYYQGRVYGYEYFKDNFNRLVQIKTKVDPNNFFRNEQSVPVISYMGSV 1086

BLAST of Tan0012971 vs. ExPASy TrEMBL
Match: A0A6N2NAL0 (Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS461726 PE=3 SV=1)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 550/1055 (52.13%), Postives = 736/1055 (69.76%), Query Frame = 0

Query: 26   SVQQSFLQCLITNSLPKFPISDAIFTPNNSSFLPILNSYIRNLRFQTPTTPKPLVIVAAK 85
            SV +SFLQCL +N+ P+  IS+ +++ +++S+  +L +YIRN R+ T  TPKP+VIV   
Sbjct: 30   SVYESFLQCLESNTNPQDKISNLVYSQSSASYTSVLRAYIRNARYNTSATPKPVVIVTPT 89

Query: 86   HQSHVQSTVVCAKRVGLQIRIRSGGHDYEGLSYVSQQPFIILDLFNLRAIKVNIAKGTAR 145
              SHVQ+TV+C K+VG Q++IRSGGHDY+G+SYVS  PF +LD+FNLR+I V++   +A 
Sbjct: 90   QISHVQATVICTKKVGYQLKIRSGGHDYDGISYVSDMPFFVLDMFNLRSIAVDVKDESAT 149

Query: 146  VQAGATMGELYYAIANRSNVHGFPGGICPTLGAGGHLSGGGYGNLMRKFGLSIDNILDAQ 205
            VQ GAT+GELYY I   S VHGFP GICPT+G GGHLSG GYGN++RK+GLS+DN++DA+
Sbjct: 150  VQTGATLGELYYKIWESSKVHGFPAGICPTVGVGGHLSGAGYGNMLRKYGLSVDNVVDAE 209

Query: 206  IVNVQGKIRNRQKMGEDLFWAIRGGGGGSFGVILSWKIKLVPVPSTVTVFKVSRKIEEGA 265
            IV+V GK+ +R+ MGEDLFWAIRGGGGGSFGVI+S+KIKLVPVP TVTVF+V R +E+ A
Sbjct: 210  IVDVNGKLLDRKAMGEDLFWAIRGGGGGSFGVIISYKIKLVPVPETVTVFRVERTLEQNA 269

Query: 266  TDVVWQWQQVMDKLDENLYIRLMLHSSKGKNGQKTGKASLVALFLGPAEKLMEIVNKNIP 325
            TD+V++WQ V  +   +L++R++L     +NG++T +AS+V L+LG ++ L+ ++ K  P
Sbjct: 270  TDIVYKWQLVAPQTSNDLFMRMLLQPVT-RNGKQTLRASIVTLYLGNSDSLVALLGKEFP 329

Query: 326  SLKLQKQECIEMSWIESVLFWANFPN-GTTPEALLSRTIPSIPFLKRKSDYVREPISREG 385
             L L+K+ C E SWI+SV++W    N G +P+ LL R      FLKRKSDYV+ PIS++G
Sbjct: 330  ELGLKKENCNETSWIQSVMWWDESTNLGKSPDVLLDRNPNDANFLKRKSDYVQNPISKDG 389

Query: 386  IEAIWKAIMEIEEVGLVWNPYGGRMSQISETATPFPHRAGVKFKIQYSANWKKGGDEETN 445
            +E +WK ++EI + GLV+NPYGGRMS+I  + TPFPHRAG  FK+QYS NW++ G E   
Sbjct: 390  LEWLWKKMIEIGKTGLVFNPYGGRMSEIPASETPFPHRAGNLFKVQYSVNWEEAGGEADK 449

Query: 446  ENINLSRRLYEVMTPYVTKNPREAFLNYRDIDVG--STTHWSVEEGRVYGAKYFKGNFER 505
              +   RRL+  MTP+V+KNPR ++LNYRD+D+G       S E+G VYG KYF  NF+R
Sbjct: 450  NFMTQIRRLHSYMTPFVSKNPRSSYLNYRDLDIGVMEAGKDSFEQGSVYGYKYFNDNFDR 509

Query: 506  LVDVKTRLPMA--IPNSLLIPIL---------------------------------TTLL 565
            LV VKT +       N   IP L                                 TT  
Sbjct: 510  LVKVKTAVDPENFFRNEQSIPTLPKVGQDTGVAGGSTSRFIQNFVCSKSDRNENVSTTSK 569

Query: 566  LLLISMA-ASDQIPSHQTLLQCLSDHSSPSSSPISELVFFPDNPSYSPVLNSYIRNLRFA 625
             + IS+A ++    S +  L+C S     S S ISE +    + +YS VL S IRN RF 
Sbjct: 570  PISISLAWSASSSSSPEMFLRCFSSRMQHSKS-ISEAILTKKSSAYSSVLQSSIRNFRFL 629

Query: 626  SPTTPKPLFIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDMFN 685
            + +T KP FI+ P +   IQA ++C + + ++IR+RSGGHDY+GLS +SD  FV++D+  
Sbjct: 630  NTSTLKPQFIITPFNEFEIQAAVVCAKKYGMQIRVRSGGHDYEGLSSLSDQEFVLVDLAE 689

Query: 686  LRSVAVDIEDESAWVESGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLM 745
            L S++VDIE+E AW+ +GA++GE+Y++IAEKSK+HGFPAG CPTVGVGG  SG G+G + 
Sbjct: 690  LSSISVDIENERAWIGAGASIGELYYRIAEKSKVHGFPAGSCPTVGVGGQFSGGGFGTIF 749

Query: 746  RKFGVSVDNVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGVIVSWEFKLVPLPET 805
            RK+G++ DNV+DA IVD NG +LDRESMGEDLFWAIRGGG ASFGV+ SW+ +LVP+P T
Sbjct: 750  RKYGLAADNVIDARIVDANGIILDRESMGEDLFWAIRGGGAASFGVVFSWKVRLVPVPPT 809

Query: 806  VTVFRIEKTMEEGAVDILYKWQEVADQIDVNLFIRVVILPVNK--KTQKTAKAKFISLFL 865
            VTVF I KT+++GA  +L KWQ + D++  +LF+   I          KT +  F+SLFL
Sbjct: 810  VTVFSIGKTLQQGASSLLQKWQSIGDKLHQDLFLHATISVATSIPDGNKTIQVSFVSLFL 869

Query: 866  GTAQELFALMSENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTSLNVLLERQPNSEKFLK 925
            G A+EL  LM   FPELG+  E+C EMSWI SVL++  +    SLNVLL R    ++F K
Sbjct: 870  GRAEELLPLMQGTFPELGLMRENCSEMSWIQSVLYFGGFSPRDSLNVLLSRTTQFKEFSK 929

Query: 926  KKSDYVQEPISKANLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQ 985
             KSDYV++PIS+  LEG+ + ++E +   L   PYGG+MS+IP +ETPFPHR+GN +KIQ
Sbjct: 930  GKSDYVKQPISETGLEGLYKMLLEEETSLLILTPYGGRMSEIPSSETPFPHRSGNIFKIQ 989

Query: 986  YSVTWKEEGDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGNASYKQA 1040
            Y +TW  E  E   KNL+ +R LY YM PYVS SPR+AYLNYRD+DLG N  GN S+ +A
Sbjct: 990  YIITWDVE--EETEKNLKWMRRLYAYMAPYVSSSPRAAYLNYRDLDLGRNSYGNTSFAKA 1049

BLAST of Tan0012971 vs. ExPASy TrEMBL
Match: A0A7J6G9H6 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_028769 PE=3 SV=1)

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 556/1037 (53.62%), Postives = 740/1037 (71.36%), Query Frame = 0

Query: 28   QQSFLQCLITNS-LPKFPISDAIFTPNNSSFLPILNSYIRNLRFQTPTTPKPLVIVAAKH 87
            Q+SFLQCL T S     PIS   F P++ S+  +LNSYIRNL F  PT+PKP  IVA  H
Sbjct: 9    QKSFLQCLSTYSPTSPLPISSVTFFPDDPSYPSVLNSYIRNLIFMLPTSPKPEFIVAPTH 68

Query: 88   QSHVQSTVVCAKRVGLQIRIRSGGHDYEGLSYVSQQPFIILDLFNLRAIKVNIAKGTARV 147
             SH+Q+ +VC K  GLQ+RIRSGGHDY+GLSYVS  PF+I+D+FNLR I V+    TA V
Sbjct: 69   VSHIQAAIVCCKIHGLQVRIRSGGHDYDGLSYVSNDPFVIIDMFNLRNITVSTEDETAWV 128

Query: 148  QAGATMGELYYAIANRSNVHGFPGGICPTLGAGGHLSGGGYGNLMRKFGLSIDNILDAQI 207
            ++GAT+GELYY IA +S +HGFP G+CPT+G GGH SGGGYGNLMRK GL++DNI+DA I
Sbjct: 129  ESGATIGELYYRIAEKSKIHGFPAGVCPTVGVGGHFSGGGYGNLMRKHGLTVDNIIDAVI 188

Query: 208  VNVQGKIRNRQKMGEDLFWAIRGGGGGSFGVILSWKIKLVPVPSTVTVFKVSRKIEEGAT 267
            V+V G+I NR  MGEDLFWAIRGGGG SFGVI+SWKIKLV VP  VTVF+V R +EEGAT
Sbjct: 189  VDVNGRILNRADMGEDLFWAIRGGGGASFGVIVSWKIKLVSVPEKVTVFRVVRTLEEGAT 248

Query: 268  DVVWQWQQVMDKLDENLYIRLMLHSSKGKNGQKTGKASLVALFLGPAEKLMEIVNKNIPS 327
            D+V QWQ V D+L   ++IR++L     K  QKT KA  VALFLG  E++  ++ K  P 
Sbjct: 249  DMVLQWQDVADQLLHEMFIRVVL-MPVNKKTQKTVKAKFVALFLGNKEQIGVLMEKKFPK 308

Query: 328  LKLQKQECIEMSWIESVLFWANFPNGTTPEALLSRTIPSIPFLKRKSDYVREPISREGIE 387
            L L+ ++ IEMSWIESVLFW+N+P GT+   LL R   S  FLK+KSDYV+EPI R+ +E
Sbjct: 309  LGLKPEDYIEMSWIESVLFWSNYPIGTSMNVLLERQPKSEKFLKKKSDYVQEPIQRDALE 368

Query: 388  AIWKAIMEIEEVGLVWNPYGGRMSQISETATPFPHRAGVKFKIQYSANWKKGGDEETNEN 447
             +WK ++E+++  L +NPYGG+MS+ISE  TPFPHRAG K+KIQYS  WK+   E  ++N
Sbjct: 369  GLWKKMIELKKPALTFNPYGGKMSEISELETPFPHRAGNKYKIQYSVQWKEEDSESLDQN 428

Query: 448  INLSRRLYEVMTPYVTKNPREAFLNYRDIDVGST---THWSVEEGRVYGAKYFKGNFER- 507
            I+  R+LY+ MTPYV+K+PR ++LNYRD+D+G+     + +  +   +G KYFKGNF+R 
Sbjct: 429  IDRIRKLYDHMTPYVSKSPRCSYLNYRDVDLGTNGVGYNANYSQASTWGTKYFKGNFDRW 488

Query: 508  LVDVKTRLPMAIP-----NSLLIPILTTLLLLLISMAASDQI-----PSHQT----LLQC 567
            L + +  + +A P       ++  I  TL+ +L+++  S  +     P++++     LQC
Sbjct: 489  LEEDEEGVGLACPPPNYHMEIIKMIPLTLIFVLLNIFYSSSLLAGSTPANESYYLPFLQC 548

Query: 568  LSDHSSPSSSPISELVFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQAT 627
            +   +S  S  + ++++   NPSY+ VL +YIRN RF + +T KP  I+ P   S + A 
Sbjct: 549  M--QNSTKSIKVKDIIYNEANPSYTSVLRAYIRNSRFNTSSTLKPSLIITPATESDVAAA 608

Query: 628  ILCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDMFNLRSVAVDIEDESAWVESGATLG 687
            +LC +  +L ++ RSGGHDY G+SY S  PFVILDMF LRSV VD+ D+SAWV++GATLG
Sbjct: 609  VLCTKATSLRLKTRSGGHDYAGVSYTSTVPFVILDMFQLRSVTVDVADQSAWVQAGATLG 668

Query: 688  EVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRV 747
            E+Y+ I  KSK+ GFPAG+CPTVG GGH+SG GYGN++RK+G++ D V+DA IVD  G +
Sbjct: 669  ELYYNIWNKSKVLGFPAGICPTVGAGGHISGGGYGNMIRKYGLASDYVIDAKIVDAKGNI 728

Query: 748  LDRESMGEDLFWAIRGGGGASFGVIVSWEFKLVPLPETVTVFRIEKTME---EGAVDILY 807
            LDR+ MGEDLFWAIRGGGGASFGVI+S++ KLV +PETVTVF  E+++E   +   D + 
Sbjct: 729  LDRKQMGEDLFWAIRGGGGASFGVILSYKIKLVQVPETVTVFNAERSLENKNDSMTDFVV 788

Query: 808  KWQEVADQIDVNLFIRVVILPVNK---KTQKTAKAKFISLFLGTAQELFALMSENFPELG 867
            +WQ+VA   D  LF+R+++ P      K +KT K   ++ +LG A  L ++M + FP LG
Sbjct: 789  QWQKVAPTTDEGLFMRMLVQPSTSTVTKGEKTMKVTIVAQYLGNADTLISVMGKEFPLLG 848

Query: 868  VTAEDCKEMSWIDSVLFWSNYPIGTSL-NVLLERQPNSEKFLKKKSDYVQEPISKANLEG 927
            +  EDCKE+SWI+SVL+W      T+   VLL+R  NS  F  +KSDYVQ+ IS   LE 
Sbjct: 849  LKKEDCKELSWINSVLWWLKLKNDTATPEVLLDRNVNSAIFGIRKSDYVQKEISATVLES 908

Query: 928  MMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAAAKNL 987
            + +KM   K   L FNPYGGKM+++P TETPFPHRAGN +K+QYSV WKE+  +A +K L
Sbjct: 909  IWKKMTPGK-VGLVFNPYGGKMNKVPSTETPFPHRAGNLFKVQYSVGWKEDEAQAESKYL 968

Query: 988  EVIRELYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGNASYKQASTWGRKYFKGNFDRLVK 1039
            E IR LY +MTPYVS++PRSA+LNYRD+D+G+N  G  SY++   +G KYF  NFDRLVK
Sbjct: 969  EEIRSLYSFMTPYVSQNPRSAFLNYRDLDIGINNFGKNSYEEGKVYGVKYFGNNFDRLVK 1028

BLAST of Tan0012971 vs. ExPASy TrEMBL
Match: A0A072UPP4 (FAD-binding berberine family protein OS=Medicago truncatula OX=3880 GN=MTR_4g094515 PE=3 SV=1)

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 563/1143 (49.26%), Postives = 763/1143 (66.75%), Query Frame = 0

Query: 6    SSILTFFSVILILSPWLSFA-----SVQQSFLQCLITNSLPKFPISDAIFTPNNSSFLPI 65
            SS      ++L+L+  +S A     SV  SFL+CL  ++ P   +S+ +F+  N SF  I
Sbjct: 10   SSRFFLLLLLLLLNVTVSEASPTEESVYTSFLECLTNHTNPSDQVSNILFSQTNVSFSSI 69

Query: 66   LNSYIRNLRFQTPTTPKPLVIVAAKHQSHVQSTVVCAKRVGLQIRIRSGGHDYEGLSYVS 125
            L +YIRN R    +TPKPL+I+    QSHVQ+T++CAK + +Q+++RSGGHD+EG+SYVS
Sbjct: 70   LQAYIRNARLNKTSTPKPLLIITPLQQSHVQATILCAKAIKIQLKVRSGGHDFEGISYVS 129

Query: 126  QQPFIILDLFNLRAIKVNIAKGTARVQAGATMGELYYAIANRSNVHGFPGGICPTLGAGG 185
             QPFIILD+F  R I V+I    A VQ+GA +GE+YY I  +S VHGFP G+C T+G GG
Sbjct: 130  NQPFIILDMFQFRNITVDIQNEVAVVQSGAILGEVYYRIWEKSKVHGFPAGVCITVGVGG 189

Query: 186  HLSGGGYGNLMRKFGLSIDNILDAQIVNVQGKIRNRQKMGEDLFWAIRGGGGGSFGVILS 245
            HLSGGGYGN+MRKFGLSIDN++DA+IV+V G+I +R+ MGE+LFWAIRGGGG SFGVILS
Sbjct: 190  HLSGGGYGNMMRKFGLSIDNVVDAEIVDVNGRILDRKSMGEELFWAIRGGGGASFGVILS 249

Query: 246  WKIKLVPVPSTVTVFKVSR---KIEEGATDVVWQWQQVMDKLDENLYIRLMLH--SSKGK 305
            + IKLV VP  VTVF+V      +++  T+ V QWQ V  + D+ L++RL +   SSK  
Sbjct: 250  YSIKLVQVPEVVTVFRVEESLDSLDQNITEFVLQWQNVAPRTDDRLFMRLHIQPASSKVV 309

Query: 306  NGQKTGKASLVALFLGPAEKLMEIVNKNIPSLKLQKQECIEMSWIESVLFWANFPNGTTP 365
             G+ T + S++ALFLG A++L+ ++ K  PSL L+K+ C EMSWI S L+W    + + P
Sbjct: 310  KGEITVRVSVMALFLGGADELVTLLGKEFPSLGLKKENCSEMSWIGSALWW-GISSNSKP 369

Query: 366  EALLSRTIPSIPFLKRKSDYVREPISREGIEAIWKAIMEIEEVGLVWNPYGGRMSQISET 425
            + LL R I S  FLKRKSDYV  PIS++G+E I+K ++E+ +VGL +NPYGG+M+ I+  
Sbjct: 370  DELLDRNIDSAKFLKRKSDYVENPISKDGLEGIYKKLIELGKVGLTFNPYGGKMNSIASN 429

Query: 426  ATPFPHRAGVKFKIQYSANWKKGGDEETNENINLSRRLYEVMTPYVTKNPREAFLNYRDI 485
            AT FPHRAG  FKIQYS NW K G +  N   N +R LY+ MTP+V+ NPR AFLNYRD+
Sbjct: 430  ATAFPHRAGNLFKIQYSVNWDKPGIDIENNFTNQARMLYDYMTPFVSNNPRRAFLNYRDL 489

Query: 486  DVGSTT--HWSVEEGRVYGAKYFKGNFERLVDVKT--------RLPMAIP---------- 545
            D+G+    + S E+G VYG KYF  NFERLV +KT        R   +IP          
Sbjct: 490  DIGTNDFGNNSYEQGVVYGMKYFSSNFERLVKIKTAVDPENFFRNEQSIPTHPGSDSGAS 549

Query: 546  ----------------------------------------------------NSLLIPIL 605
                                                                 +L++ + 
Sbjct: 550  TSSADKVLLSSMRGKLMVKVIDLNELDTLKLIHTKDTNFCGPICLLYKVLTSTTLILCLF 609

Query: 606  TT------------------LLLLLISMAASDQIPSHQ----TLLQCLSDHSSPSSSPIS 665
            TT                  ++LLL+ ++ S   PS +    T LQCL +H++   + +S
Sbjct: 610  TTISRIAMDKSNSVPFLSVFIILLLVHVSNSHAAPSSESIYVTFLQCLKNHTT-QPNQVS 669

Query: 666  ELVFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQATILCCRIHALEIRI 725
             +V+   N SY+ +  ++IRN RF +P+T KPL I+ P H   +QAT+LC +   L ++I
Sbjct: 670  NIVYSQTNASYTSIFQAFIRNARFNTPSTTKPLLIITPLHEDQVQATVLCSKTIGLHLKI 729

Query: 726  RSGGHDYDGLSYVSDSPFVILDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAEKSKIH 785
            RSGGHD++G+SY+SD+PF+ILDMFN +++ VDI++E A +++GA+LG+VY++I EKSK+H
Sbjct: 730  RSGGHDFEGISYISDTPFIILDMFNFQNITVDIQNEIAIIQTGASLGQVYYRIWEKSKVH 789

Query: 786  GFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRESMGEDLFWA 845
            GFPAGVCPTVGVGGHLSG GYGN+MRKFG+S+DN+VDA I+DVNGR+LD+++MGEDLFWA
Sbjct: 790  GFPAGVCPTVGVGGHLSGGGYGNMMRKFGLSIDNIVDAKIIDVNGRILDKKTMGEDLFWA 849

Query: 846  IRGGGGASFGVIVSWEFKLVPLPETVTVFRIEKTMEEGAVDILYKWQEVADQIDVNLFIR 905
            IRGGGGASFGVI+S+  KLV +PE VTVF+++K++E+ A DI+++WQ+VA   D  LF+R
Sbjct: 850  IRGGGGASFGVILSYTVKLVSVPEKVTVFQVDKSLEQNATDIVFQWQQVAPHTDDRLFMR 909

Query: 906  VVILPVNKKT---QKTAKAKFISLFLGTAQELFALMSENFPELGVTAEDCKEMSWIDSVL 965
            +V+ PVN K    +KT  A   ++FLG A EL  L  + FP LG+  E CKEMSWI+SV 
Sbjct: 910  LVLQPVNSKIVKGKKTVSASIQAMFLGGADELVTLFGKEFPLLGLKKEICKEMSWIESVF 969

Query: 966  FWSNYPIGTSLNVLLERQPNSEKFLKKKSDYVQEPISKANLEGMMRKMIELKRPALTFNP 1025
            +W+NY  G+SL  LL+R      F K+KSDYV+ PI K  L+ +M K+IEL +  L FNP
Sbjct: 970  YWANYNDGSSLITLLDRSHYKVHFSKRKSDYVKSPIPKDGLKWIMNKLIELGKVELVFNP 1029

Query: 1026 YGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAAAKNLEVIRELYEYMTPYVSKS 1042
            YGGKMS+I    T F HRAGN YKIQY+ +W+          +  IR +Y YMTP+VSK+
Sbjct: 1030 YGGKMSEISSAATAFAHRAGNLYKIQYTASWENSAAGMEKSFISQIRMMYSYMTPFVSKN 1089

BLAST of Tan0012971 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 591.3 bits (1523), Expect = 1.6e-168
Identity = 287/509 (56.39%), Postives = 375/509 (73.67%), Query Frame = 0

Query: 535  ASDQIPSHQTLLQCLSDHSSPSSSPISELVFFPDNPSYSPVLNSYIRNLRFASPTTPKPL 594
            +SD I  +++ +QC SD +    + I++ VF   NPS+S VL +YIRN RF + +TPKP 
Sbjct: 30   SSDSI--YESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRNGRFNTSSTPKPA 89

Query: 595  FIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDMFNLRSVAVDI 654
             IV P    H+ A + C +     ++IRSGGHDY+GLSY+SD PF ILDM NLR V+VDI
Sbjct: 90   IIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISDKPFFILDMSNLRDVSVDI 149

Query: 655  EDESAWVESGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVD 714
             D+SAW+ +GATLGEVY++I EKSK+HGFPAGVCPTVGVGGH+SG GYGN++RKFG+SVD
Sbjct: 150  ADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGYGNMLRKFGLSVD 209

Query: 715  NVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGVIVSWEFKLVPLPETVTVFRIEK 774
            N++DA IVDVNG++LDR+SMGEDLFWAI GGGGASFGV++ ++ KLVP+PETVTVFR+EK
Sbjct: 210  NLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVPVPETVTVFRVEK 269

Query: 775  TMEEGAVDILYKWQEVADQIDVNLFIRVVILPVNKKTQKTAKAKFISLFLGTAQELFALM 834
             M+ GAVD+++KWQ V  + D NLF+R++I PV +K  KT +A  ++LFLG A+E+ AL+
Sbjct: 270  YMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQPVTRKKVKTVRATVVALFLGRAEEVVALL 329

Query: 835  SENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTSLN--VLLERQPNSEKFLKKKSDYVQE 894
             + FPEL +  E+C EM+W  S L+W N    T ++  V L+R  +   F K+KSDYV  
Sbjct: 330  GKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNLDRANFGKRKSDYVAS 389

Query: 895  PISKANLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEE 954
             I +  +E + +KM EL +  L FNPYGGKM+++    TPFPHR+   +KIQYSVTW+E 
Sbjct: 390  EIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS-KLFKIQYSVTWQEN 449

Query: 955  GDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGNASYKQASTWGRKYF 1014
              E     L     LY +MT +VSK+PR+AYLNYRDVD+GVN +G  SY++   +GRKYF
Sbjct: 450  SVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDHGTNSYEEGEVYGRKYF 509

Query: 1015 KGNFDRLVKVKTMVDPDNFFRYEQSIPSV 1042
              NFDRLVKVKT  DPDNFFR EQSIP+V
Sbjct: 510  GDNFDRLVKVKTAADPDNFFRNEQSIPTV 535

BLAST of Tan0012971 vs. TAIR 10
Match: AT1G34575.1 (FAD-binding Berberine family protein )

HSP 1 Score: 577.0 bits (1486), Expect = 3.0e-164
Identity = 288/514 (56.03%), Postives = 379/514 (73.74%), Query Frame = 0

Query: 4   SSSSILTFFSVILILSPWLSFAS---VQQSFLQCLITNSLPKFPISDAIFTPNNSSFLPI 63
           SS++    F+V L+L P  SFA+   ++ SF QC +T   P  PI +  +T  N +FL I
Sbjct: 3   SSTTQTLIFTVFLLLIP-TSFAAPPKLKDSFTQC-VTVFKPSVPIQNFTYTQQNPNFLTI 62

Query: 64  LNSYIRNLRFQTPTTPKPLVIVAAKHQSHVQSTVVCAKRVGLQIRIRSGGHDYEGLSYVS 123
           LN+Y+RNLR+   TT KP+ IVAA H +H+Q+T+ CAK++GLQ+RIRSGGHDY+G+SY+S
Sbjct: 63  LNNYVRNLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYLS 122

Query: 124 QQPFIILDLFNLRAIKVNIAKGTARVQAGATMGELYYAIANRS-NVHGFPGGICPTLGAG 183
              F++LD+FNLRAI+++    TA VQ+GAT+GE+YY +AN+S N+ GFP GICP LGAG
Sbjct: 123 TVDFVVLDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPGLGAG 182

Query: 184 GHLSGGGYGNLMRKFGLSIDNILDAQIVNVQGKIRNRQKMGEDLFWAIRGGGGGSFGVIL 243
           GH SGGGYGN+MRK+GLSIDNI+DA+IV+   ++ +R  MGEDLFWA+RGGG  SF V+L
Sbjct: 183 GHFSGGGYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCVVL 242

Query: 244 SWKIKLVPVPSTVTVFKVSRKIEEGA--TDVVWQWQQVMDKLDENLYIRLMLHSSKGKNG 303
           +WKIKLVPVP  VTVF V      G   TD+  +WQ++ DK+D +L+IRL L SS     
Sbjct: 243 AWKIKLVPVPEKVTVFNVETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTLSSS----- 302

Query: 304 QKTGKASLVALFLGPAEKLMEIVNKNIPSLKLQKQECIEMSWIESVLFWANFPNGTTPEA 363
            KT KAS + ++LG +EKL+EI+N   P L L K ECIEM WIESVLFW + P GT P +
Sbjct: 303 NKTVKASFMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTS 362

Query: 364 LLSRTIPSIP-FLKRKSDYVREPISREGIEAIWKAIMEIEEVGLVWNPYGGRMSQISETA 423
           ++   IP    +LKRKSDYV++PIS+ G+E+I+K + E E V + WNPYGGRMS+I  T 
Sbjct: 363 VMLNRIPQKQIYLKRKSDYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSEIPATE 422

Query: 424 TPFPHRAGVKFKIQYSANWKKGGDEETNENINLSRRLYEVMTPYVTKNPREAFLNYRDID 483
           T FPHRAG  FKIQYS+NW   G+E  ++ ++ + R++E M+PYV+KNPREAFLNYRDID
Sbjct: 423 TAFPHRAGNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDID 482

Query: 484 VGSTTHWSVEEGRVYGAKYFKGNFERLVDVKTRL 511
           +G   + + EEG+VYG KYFK NFERLV VKTR+
Sbjct: 483 IGKNLNSTYEEGKVYGVKYFKNNFERLVQVKTRV 509

BLAST of Tan0012971 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 576.6 bits (1485), Expect = 4.0e-164
Identity = 283/524 (54.01%), Postives = 383/524 (73.09%), Query Frame = 0

Query: 522  ILTTLLLLLISMAASDQIPSHQTLLQCLSDHSSPSSSPISELVFFPDNPSYSPVLNSYIR 581
            ++  L  ++    +S    + +T  QCL+ +S P   PIS  +FF  N SYS VL + IR
Sbjct: 5    LILVLFFVVFIWQSSSSSANSETFTQCLTSNSDP-KHPISPAIFFSGNGSYSSVLQANIR 64

Query: 582  NLRFASPTTPKPLFIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLSYV--SDSPF 641
            NLRF + +TPKP  I+A TH SH+QA I C + H L+++IRSGGHDYDGLSYV  S  PF
Sbjct: 65   NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 124

Query: 642  VILDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSG 701
             +LDMFNLRSV VD+  ++AWV++GA LGEVY+ I EKSK   +PAG+CPTVGVGGH+SG
Sbjct: 125  FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 184

Query: 702  AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGVIVSWEFK 761
             GYGN+MRK+G++VDN +DA +VDVNG++LDR+ MGEDL+WAI GGGG S+GV+++++  
Sbjct: 185  GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 244

Query: 762  LVPLPETVTVFRIEKTMEEGAVDILYKWQEVADQIDVNLFIRVVILPVN--KKTQKTAKA 821
            LV +PE VTVFRI +T+E+ A DI+++WQ+VA ++   LFIR VI  VN    +QKT + 
Sbjct: 245  LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRT 304

Query: 822  KFISLFLGTAQELFALMSENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTSLNVLLERQP 881
             FI++FLG    L ++++  FPELG+   DC E SWI SVLFW+N  +G+S  +LL+R  
Sbjct: 305  TFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN- 364

Query: 882  NSEKFLKKKSDYVQEPISKANLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRA 941
                +LK+KSDYV+EPIS+  LE + +KMIEL+ P + FNPYGG+M +I  T TPFP+RA
Sbjct: 365  QPVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRA 424

Query: 942  GNKYKIQYSVTWKEEGDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNYRDVDLGVNG-N 1001
            GN +KIQY   W++E      + +E+ R+LY++MTP+VSK+PR ++ NYRDVDLG+N  N
Sbjct: 425  GNLWKIQYGANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHN 484

Query: 1002 GN-ASYKQASTWGRKYFKGNFDRLVKVKTMVDPDNFFRYEQSIP 1040
            G  +SY +   +G+KYF GNF+RLVK+KT VD  NFFR EQSIP
Sbjct: 485  GKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of Tan0012971 vs. TAIR 10
Match: AT4G20830.1 (FAD-binding Berberine family protein )

HSP 1 Score: 576.2 bits (1484), Expect = 5.2e-164
Identity = 284/519 (54.72%), Postives = 372/519 (71.68%), Query Frame = 0

Query: 526  LLLLLISMAASDQIPS-HQTLLQCLSDHSSPSSSPISELVFFPDNPSYSPVLNSYIRNLR 585
            LL L + +++  Q  S + + L+C SD +    S I++ VF   NP++S VL +YIRN R
Sbjct: 19   LLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNAR 78

Query: 586  FASPTTPKPLFIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDM 645
            F + +T KP  I+ P   SH+ A + C +     ++IRSGGHDYDGLSY+SD PF ILDM
Sbjct: 79   FNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDKPFFILDM 138

Query: 646  FNLRSVAVDIEDESAWVESGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGN 705
             N+R V+VDI   SAW+ +GATLGEVY++I EKS++HGFPAGVCPTVGVGGHLSG GYGN
Sbjct: 139  SNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGYGN 198

Query: 706  LMRKFGVSVDNVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGVIVSWEFKLVPLP 765
            ++RKFG+SVD V DA IVDVNGRVLDR++MGEDLFWAI GGGG S+GV++ ++ KLVP+P
Sbjct: 199  MVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPVP 258

Query: 766  ETVTVFRIEKTMEEGAVDILYKWQEVADQIDVNLFIRVVILPVNKKTQKTAKAKFISLFL 825
              VTVFR+E+ M+ GAVD+++KWQ V  + D NLF+R++I PV +K  KT +A  ++LFL
Sbjct: 259  SVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVTRKKVKTVRASVVALFL 318

Query: 826  GTAQELFALMSENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTSLN--VLLERQPNSEKF 885
            G A E+ AL+S+ FPELG+  E+C EM+W  S L+W N    T ++  V L+R  ++  F
Sbjct: 319  GRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLDTSSF 378

Query: 886  LKKKSDYVQEPISKANLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYK 945
             K+KSDYV   I K  +E + +KMIEL +  L FNPYGGKM+++     PFPHR    +K
Sbjct: 379  GKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-NKLFK 438

Query: 946  IQYSVTWKEEGDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGNASYK 1005
            IQYSV WKE   E     L   + LY +MT +VSK+PRS+Y NYRDVD+GVN +G  SYK
Sbjct: 439  IQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGANSYK 498

Query: 1006 QASTWGRKYFKGNFDRLVKVKTMVDPDNFFRYEQSIPSV 1042
            +   +GRKYF  NFDRLVK+KT VDP NFFR EQSIP++
Sbjct: 499  EGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTL 536

BLAST of Tan0012971 vs. TAIR 10
Match: AT4G20830.2 (FAD-binding Berberine family protein )

HSP 1 Score: 576.2 bits (1484), Expect = 5.2e-164
Identity = 284/519 (54.72%), Postives = 372/519 (71.68%), Query Frame = 0

Query: 526  LLLLLISMAASDQIPS-HQTLLQCLSDHSSPSSSPISELVFFPDNPSYSPVLNSYIRNLR 585
            LL L + +++  Q  S + + L+C SD +    S I++ VF   NP++S VL +YIRN R
Sbjct: 19   LLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNAR 78

Query: 586  FASPTTPKPLFIVAPTHISHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDM 645
            F + +T KP  I+ P   SH+ A + C +     ++IRSGGHDYDGLSY+SD PF ILDM
Sbjct: 79   FNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDKPFFILDM 138

Query: 646  FNLRSVAVDIEDESAWVESGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGN 705
             N+R V+VDI   SAW+ +GATLGEVY++I EKS++HGFPAGVCPTVGVGGHLSG GYGN
Sbjct: 139  SNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGYGN 198

Query: 706  LMRKFGVSVDNVVDALIVDVNGRVLDRESMGEDLFWAIRGGGGASFGVIVSWEFKLVPLP 765
            ++RKFG+SVD V DA IVDVNGRVLDR++MGEDLFWAI GGGG S+GV++ ++ KLVP+P
Sbjct: 199  MVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPVP 258

Query: 766  ETVTVFRIEKTMEEGAVDILYKWQEVADQIDVNLFIRVVILPVNKKTQKTAKAKFISLFL 825
              VTVFR+E+ M+ GAVD+++KWQ V  + D NLF+R++I PV +K  KT +A  ++LFL
Sbjct: 259  SVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVTRKKVKTVRASVVALFL 318

Query: 826  GTAQELFALMSENFPELGVTAEDCKEMSWIDSVLFWSNYPIGTSLN--VLLERQPNSEKF 885
            G A E+ AL+S+ FPELG+  E+C EM+W  S L+W N    T ++  V L+R  ++  F
Sbjct: 319  GRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLDTSSF 378

Query: 886  LKKKSDYVQEPISKANLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYK 945
             K+KSDYV   I K  +E + +KMIEL +  L FNPYGGKM+++     PFPHR    +K
Sbjct: 379  GKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-NKLFK 438

Query: 946  IQYSVTWKEEGDEAAAKNLEVIRELYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGNASYK 1005
            IQYSV WKE   E     L   + LY +MT +VSK+PRS+Y NYRDVD+GVN +G  SYK
Sbjct: 439  IQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGANSYK 498

Query: 1006 QASTWGRKYFKGNFDRLVKVKTMVDPDNFFRYEQSIPSV 1042
            +   +GRKYF  NFDRLVK+KT VDP NFFR EQSIP++
Sbjct: 499  EGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTL 536