Tan0012962 (gene) Snake gourd v1

Overview
NameTan0012962
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionRibonuclease T(2)
LocationLG01: 4797840 .. 4805504 (-)
RNA-Seq ExpressionTan0012962
SyntenyTan0012962
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATGGTCTCTTTCTCATTCAAAGCGGCGGCGCCGGTGGTGTTTCCGGTTCTTGCTGTTGTTGCTCAGCTGCAGGTGGCGGCGCTTGCGGCAGAGGGCCTCACCGGCCGGCCGGAGCAGCGGGAATTTGACTACTTTGTATTGGCTCTTCAATGGCCAGGCACTTGCTGCAGAAATGTAACCGTGTGTTGCCCCTACAATGGTTGCTGCCGAGGGTGAGTCAACTTTTTTTTTTTTAAGGCAAAATTGCACAAACCACGTCTAAATTATACCTCCTTACACTTTTACTTCATCAATAAGCCTACTAATGTATTTTGTTAAATGTTGCGATTAATCTTTTTTATTAGTCAATATTAATTATCCTTTAATTAGACTTTACATGTCAATAAGAACATAGTTGATCAACTAGCAATAATATATGAGTTTATGAATTATCATAGGTTCAAATCTTCTCAAAGGTTGTTGAACTCAAAAAAAAAAAAAAAAGACGTCACATTTGTGACCTCCACATTCATGAATCAAAAAAGAATAAAATTGTTATTGAGGTGTGATTGTAACAACTCCATAGTTTAGGAGTGGGGTGGCTGATAGTTAGTTTTACACTATCAGATGTATAAGAGAAAAATTATATAAGTGTTTTGAATTTATATTGAGTAGTTATTAGGGATATGCAAATTTTCAAATTCAATGGTTATTAGGAAGCCGCCAATATTGAATAATTTTTCTTATAATTTGGAGCCCATTCTATTTTAGTTTTGTACTTTTGAATGTCCAAAATTACTCTTTATACTTTTTTTTTTTGTAAATCTTAAAACTAACCTGATGTTAGTTTTAAGTTAGTTTTTATTTAAATTAGTTTGTATTAAGAAATACAATGTTAGTTTGTACTAAGAAATACAATGGAAATGTGTTTTTCAAACCTTTTATGGGAATGCTAATAAAAACAATTTTTTTTAATAAAAAAACCCTAAACTAATTTTAAGATTTAGGAAAGTTTAGGAATTAAATTTTAACATTCGAAAAACAAGTTTTAAAGTAGATTAATACTACATTAATATTTTGATTTTGATTTTTCTTTTGTTATTTTCTTTTTTCATGGGGTTTGTAGTTTGATATGTTGTGTTGTGAAATAATAATAATAATAATAATAATAATAATAATAATAATGTGCTAATTAAAATTTATCTGTCTCTCTCTAGAGCTGACTCTCTCACTGGATTTACAATCCGTAAGTTGTGAAAAAGAACTGAGGTTTGGATTTTGGTTTTGATTTGGATGTGTTGAATAATTTGGGTTCATTATTATTATTATTATTATTATTATTATTATTATTATTATTTTCATGTGGTTTTGGAAATGGTGCAGATGGACTGTGGCCTCAGTATAATGTGAAAGGATGGCCTTCATGTTGTACTAATGCAACCTTCAATGAAAATGAGGTCTCTCTCTCAAATTTAAATTTAATTTGTTGTGAAGTTGGAAAGTATATATATTACTTCGAAAAAAATAAATTTACTTTAAAAACTTTATTTTCATGCAACCTAACCATTAGGGGTGATCAAAAAACCCGATAACTCGAATAACCCGACCAACCCAACCCAACCCGACAGCTTGAGTTGGGTTATGAATTTATTTGGGTTAGGTTGGGTTCAAATAAATTAAAATGTCACGGGTTGGGTTGGTTTGGATGTGGATTCACTAAAATTAATTTGGGTTGACCACCCAACCCAAACTTATTATTAATTTTAAAAATTATATTTTTACCTACTATACAACTACATATACATGTATTTAATTTATTTGTATTTAATTTCATAATTATTTATTATTTAATAACTCTTAAAATACATTTTGTAAGTCATTAGGGTGTAATTTTTCATAATATAAATTGAAACTAAGTGGCTAATTTTAATTCAATCTTTGAGAAACGAGAGTTTTTGTTTTAAATTATGGCAATTTATTTAGAATTTTTTTGATATAATTGTAAGTTTGTAATAGAATATTTAAGTTTTATAGTTGTTATTTAGTATAAAGAAATTTGGAGTTTTTAATTCTTGAATTTTATATTTATATTAATTTTAGATTTTTTATATTTTAAATTAAAAAAAAAAAAAAGAAAAACAAAACTAACCCCATGACCCAACCCAACCCAACCTGAAAATGTTGGGTTGAGTTGGGTTGGGTTACCCTTTTGAATAGGGTTATTTGGGTTGGAAGAATAAACAACCCAAAAATTTGGGTTGGTCCAAAAAACTAACATAATTCAATCCAATTCGACTTATGTACACCCCTACTAACCATGGAAGTAAGCCAAAATCAATAAAGATATACAAATTAAATTGGTTGAATTTAGACTATGAAATTACTTAAACATGGAAGTAGAATCTTTGTGATAAGGGAGGGATAATTCCCACTATTAAATCGAGAATAGGAAACTAAAACAATATAGTAAATTGGAGACCATAACTTAAATATACATATATATCTTAGAATTTGTCATTTAATTTACTACTCACATATAAATTAATTATATATTAATTCCAACATATTATCTATATGTCACCCTCATATTTGTGATGGAATTAATAATTTAAAAGAATATGTTTTTAAATTTTATTTTTTAAAGTTACCTCTCTTGCCGAGAGTATAAAATGCCGATGTTGACAAATTTTTCTAAAAAGATCATTAAAAAAAATAATAATGGTAATGAGTAAGTGTTTAAACTAATTCATATACATTTTACACATAAGTTAAAAAAGCTTATTATCAATATTAGGTTAAACTACCAGTTTAATATATGAAGTTTTATTCTGGTGTCTTATACATTTCTCATTTCTAAAAGGAAACTAATAAGTTCCTCCATTCTTAACTTTCTACCTAATAGATTCTTGAGTTTACAATCTTTTGTCTAATAGGTCTCTAAATTTTAAAAATATCGAAAAATGTGTCTAAACTTTCAATTTTATGTCTAATAAATATATGTCATGTTCCATATTTTTTAAATCAATGTATCTGATAATTATAAAATTGATTTTTTTTTGTTTCATAGAACAATAAACTTTCAATTTATGTCCAATATGTTTGTGAATTTTGAAAAATATTGAATGAGTTAAGAACTTATTAGATACAAAATCGAAAAAACTCAAAAATTTAATATATACAAAATAAAAAATTTAGAAACTTAAACACATTTTTTAATGTTCATGAATCTATTAGACAAAAATTATTAGAAGTTTAGAGATTTCTAGACATATTTTAAAGTTCAAGAACTTATTTGACACATGTATGGAAGTTTTAGAGCCTTTTTGGTAGGCAATATGAATTTTGTTTTATGTTTTCGAATTCACTGAATTTAGTGAATATGCGTTTGACAGATGCATTTCCAAAAATTATTTTCGGATTCTGTGATCCAAATAGTGAAAATTCTGAAAACAACATTTTTATGTTTTCAAATTTGAAATTTTAAAATGTAGAATTATATTAAAAAAAGATAAAATCTTTAATAAATATAATATTTTATGTATAAACTCAATTTATTTTTGTTAAATAAAAATATGTATAGTATAGCATATTATAAATTAATGTGATATTCTAACATGATGTTTTATATTCAACTTAATATTTAATTTTATCACTACAACTAATTTCATGGTCTATAAGTATATTATAGGATACATTTTTAATGATTGCTTAAATTAGAATCCAATTTCTAAATCTGCTGACACACACACATATCTGAAAACATAAAATACAACTCTGTTTTTATTGAATTTATTGTTTTCAAAATTTTGTTTTTAGATTGCCTATCAAACACATCATTAAGAACTAAACTTCATAATTTAACTTTAACATTATATTTACATTAAGGGCATTTTGTTGTTATCTTTTTTTATGGTATATCGATTTTTTTACGGTATTTTGAAAATGTTGATGTTAAATTTGTAAACATGTAGAAATGTCAACATGCCAATGAGAATTTACTACCCTCCCCTCTCACCAATTACTTTTAATTGCACATGTATATTCTCTCTCCTAACTTCTCTCTAATTTCTAAACATACTTTTTCTGTTTAATATCTTTGAGGTTCAAACTTGCTACACGCAAAATTAAAAATTTAAAAACTTATTAATTAACCTTCTTTAAAATACCTAGAACAAATAAACACACTCTTGAGGGTTTATGAACCGATGACAATTAACTTTTTAAACTACAAGACTTATCAAACACAACTTTAAAATTTTAGAAACCAAAGCAAAACATGTAGTTTGAGTTTAACCTAAAATTAATAATAAGCCTTTTAATTAGGTGTAAAATGTATATGAATTAGTTTAAATACTTATTCATAACCATCATTATTTTTTGTATGAATTAGTTTAAATGTATACAATAAACTATATTCAAAAGATTAAATATACCTTAAATGTTGCCAACAAGAGCATAACTCAACTGACATCAAGTAAGACTTATGACTAAGAGGTCATGAGTTCGAATCTCCTACCCTCAAATGTTGTTGTACTAAAATAAATAAATAAATATATATATATACTTTAAATGCTCTCTATATATAAATTCATTGGCAAACAAGATATAAAGGTGGCATTCTTATGAATAACATTGCATTAATACTATAATTCCATGAACTTAAAAATAATGATATTTCCCAAATTAAACCAACAGTATAATTCAACAACTCTTAACCTTGATTACACTTGTAGATTAGCACAATGATTGAAGATCTTGACAAGTATTGGCCAAGTTATCGATGTGGTCTTGCAACAAGCTGCAATAATAGAAAAGTTTCATTTTGGGCAAAAGAGGTTCAAATTCTCTCTCTCTTTTCTTCATCAGCTCATTATCAAAATTTTAGACTAACCCTTTTTTTTTCTCTCCCTCTCTCTCTTGTTATGATGACCAATTTGACATGTTACATTGATATGTAGTATGGTAAGTTAATTTCTTTCACCTCTACCTTTAATGCTTTACAATAATCTTCTTCATTCTTTAAAAAAAAAAAAAAAAAATTGAAGTGTGTGATATAAAGTCAATTAAAAACACTTTTTTAAAATTTGTAATAATCAAAGTAGTACTGATTTTAAATTTCCCTTTGAAACTTTTTTGTGCAGAGAAGCATGGAACCTGTGGATCTCCTGTAATTGTGAAGGAATATGATTACTTCTCGACAACCCTCCATCTTTTCTTTAAGTACAATGTCACTGTAAGCTTTTTTAAATTAAACCTCTCTTAAATCTTTTTTTTTTTTTTTATCTATTTTCGAATATTAAAAGTTTGGTGCCCATAAATAATAATATTCATTTATATGTATTTAATTTCCTCGTCAGGAAACTCTGGAGAATGCTGGAATTTTAGCTTCGGATACTAAAAAATATCCAATAAAAGATGTGTTTGGTGCAATTGAGTCTGCTTTCAAAGCAACTCCAAAGCTTACCTGTGGTAAAAAGGGCATTATTAAGGAACTTCGGCTATGCTTCGATAAAGATTTTACGGTATTAATTGTTTTTTTATTTACCTCATATTATAAAAATTAATTAGGCCTCTTTTGTTGGACATTTTTTTTAATTTATTATAAAAAATATTATATTTTATAAATTATTTATATTAATATTGTAGTTTAATTAAGGCTCACAGGTTGCAACATAGTTGTCTCTTGAAAAGACAACTATCACACAATCATTAATCATATCAATCCTCTAAAACTCAAAGTCTTCGGTTTAAGTATTAGAAAAATATAATATTTACCTTTTTATTTTTCCCTCCACTTTTTCTACTTCTTTTTCTTTTTTAACTTATTCTTTTATTTGCTTTCTTTAAAAAATGTTTTAAGTAAATTTAATATTATATTACATTTTTCCTAGCACATCAAATATAGACTTAATCAAGAAAGTCATAAGTTCAAATCTCATGTCCCGAAATGTTGTTAAATTCATACATATTATATTGTATTTTTTTTCAACATTTTTCTAGGATATGTTTCATCTTTCTTAAGAAAATATTTGAATATTCTTAGCCAAATTTCAAAAAAAAAAAAAAAAAAAATTGAAGATTAGTTTTAAAACTTGTCTTCATTTTTTAAAACATTGGTAAAATGTAGATAACAAAATAAGGAAAATCATTGGTGGGAGTAGTATTATAAATTTAATTTTTAAAAACCAACCACCAAAAATTAAGAACTCGTTTGATAACCTCATTTGGTTTTTAAAAATTAAATCTAAAAATATTACTTATACCAATGAATTTCTATATTTTCTTATTTATTTTATGTATCTATGTTTTCAAAAACCAAACTAAGTTTTTAAATCTAAAGAAAGTAGTTTCTTACTAGATTTTGTTTTTAGAATTTCACTAAAAATTCAAATGCTTCGACAAGTGAAGATGAATCTTATTATAGAGAAATTTAGAAAAAACAATCTTAATTTTAAAAAATTAAAAATATATATATATAAAATATTATAGCTTAAATGATTATTAAATGAAACCTGAGCTCTTAGCTATTGGTTTGTTAATGGTTATCGTACGAATATAGTGATCGTAACCATGAGAGATTGATGAAAGTAATTAAATCTTTAATTGTTGCAGCCACGTAATTGTGATCTTACAAAATCATGCCCTAAATCTGTTAGGCTACCAGAATTTCCAGATCTGCCACGGGGTAAGTTTAATTTCTTTCCTTATTATATTATTGGCCAACTCCATAAAATAGATAATATAGTTGGTGTTACCAACTTCAATATTAAGTTAAATCTCAATTATTAGTATTATATTTTTTTTAGTTCAAGATGATACATCTTAATTCTGCTCACTTTCACAAATCTCAACTATTATTAGTTTTAACATACGAAGATGTTTTATGATCTAAATTCTGTATAGTTATTATTTTACAACATCATATAATTTATAATATATTATTTTATACATATTTTAATTCTAACATAAATAAAATAGGTTTCTAACATGAAATACTATACTTACCAATTTTTTTTTTAATGTATTCTACTTTTTAAATTTTCAAATTCAAAGTCAAGATATACTGAAAACTTAAAAATGTTGGTTTTAGATCATACATGGTTTGGATTACATAATCTAAAAAAAAGTTTTAAAAATAAAATTTTCCAAATGAAGAATCCATTTGATTCAGTGAATCTAAAAACATGTCACATCATTCAAATTACATACCAAACAGACTCTAAATTTTGATCTCTATAAAATCACATAATCTATACTCAAAAACTTACCCGTAGGTTCAAAAATTAATACAAGACCCAAATAAGTTGGCCTGCGACTGATATTAATTATAAAGGAAATGAAGTTGAAAAGATTAGAACACGCAAAACACGCCTTAAGCTATGGTACTATATCAAATCATCAACATACCTCAAAGTTTAAACTACTAATTAATCTATACATGTGTGTGGTGTTTATATATTTAATTGTTTTTGTTAATATTTATATTGCAAATATTGTGTGTATTAATTGCAGAGATTGGCATGGTTAAGGGAGCAATTTCATGGATGGTTGGTGCATGAAGGCAATTATCTAATTAAGTTCTTATGAGGCCTTGAAATAATATAATTAGAAATCCTTTTTAACATTTTCGTTCATTGTCTTTAATTATGCATCTTTCATAGGCATGGTCTTGTACTTTCAATT

mRNA sequence

AAAATGGTCTCTTTCTCATTCAAAGCGGCGGCGCCGGTGGTGTTTCCGGTTCTTGCTGTTGTTGCTCAGCTGCAGGTGGCGGCGCTTGCGGCAGAGGGCCTCACCGGCCGGCCGGAGCAGCGGGAATTTGACTACTTTGTATTGGCTCTTCAATGGCCAGGCACTTGCTGCAGAAATGTAACCGTGTGTTGCCCCTACAATGGTTGCTGCCGAGGAGCTGACTCTCTCACTGGATTTACAATCCATGGACTGTGGCCTCAGTATAATGTGAAAGGATGGCCTTCATGTTGTACTAATGCAACCTTCAATGAAAATGAGATTAGCACAATGATTGAAGATCTTGACAAGTATTGGCCAAGTTATCGATGTGGTCTTGCAACAAGCTGCAATAATAGAAAAGTTTCATTTTGGGCAAAAGAGTATGAGAAGCATGGAACCTGTGGATCTCCTGTAATTGTGAAGGAATATGATTACTTCTCGACAACCCTCCATCTTTTCTTTAAGTACAATGTCACTGAAACTCTGGAGAATGCTGGAATTTTAGCTTCGGATACTAAAAAATATCCAATAAAAGATGTGTTTGGTGCAATTGAGTCTGCTTTCAAAGCAACTCCAAAGCTTACCTGTGGTAAAAAGGGCATTATTAAGGAACTTCGGCTATGCTTCGATAAAGATTTTACGCCACGTAATTGTGATCTTACAAAATCATGCCCTAAATCTGTTAGGCTACCAGAATTTCCAGATCTGCCACGGGAGATTGGCATGGTTAAGGGAGCAATTTCATGGATGGTTGGTGCATGAAGGCAATTATCTAATTAAGTTCTTATGAGGCCTTGAAATAATATAATTAGAAATCCTTTTTAACATTTTCGTTCATTGTCTTTAATTATGCATCTTTCATAGGCATGGTCTTGTACTTTCAATT

Coding sequence (CDS)

ATGGTCTCTTTCTCATTCAAAGCGGCGGCGCCGGTGGTGTTTCCGGTTCTTGCTGTTGTTGCTCAGCTGCAGGTGGCGGCGCTTGCGGCAGAGGGCCTCACCGGCCGGCCGGAGCAGCGGGAATTTGACTACTTTGTATTGGCTCTTCAATGGCCAGGCACTTGCTGCAGAAATGTAACCGTGTGTTGCCCCTACAATGGTTGCTGCCGAGGAGCTGACTCTCTCACTGGATTTACAATCCATGGACTGTGGCCTCAGTATAATGTGAAAGGATGGCCTTCATGTTGTACTAATGCAACCTTCAATGAAAATGAGATTAGCACAATGATTGAAGATCTTGACAAGTATTGGCCAAGTTATCGATGTGGTCTTGCAACAAGCTGCAATAATAGAAAAGTTTCATTTTGGGCAAAAGAGTATGAGAAGCATGGAACCTGTGGATCTCCTGTAATTGTGAAGGAATATGATTACTTCTCGACAACCCTCCATCTTTTCTTTAAGTACAATGTCACTGAAACTCTGGAGAATGCTGGAATTTTAGCTTCGGATACTAAAAAATATCCAATAAAAGATGTGTTTGGTGCAATTGAGTCTGCTTTCAAAGCAACTCCAAAGCTTACCTGTGGTAAAAAGGGCATTATTAAGGAACTTCGGCTATGCTTCGATAAAGATTTTACGCCACGTAATTGTGATCTTACAAAATCATGCCCTAAATCTGTTAGGCTACCAGAATTTCCAGATCTGCCACGGGAGATTGGCATGGTTAAGGGAGCAATTTCATGGATGGTTGGTGCATGA

Protein sequence

MVSFSFKAAAPVVFPVLAVVAQLQVAALAAEGLTGRPEQREFDYFVLALQWPGTCCRNVTVCCPYNGCCRGADSLTGFTIHGLWPQYNVKGWPSCCTNATFNENEISTMIEDLDKYWPSYRCGLATSCNNRKVSFWAKEYEKHGTCGSPVIVKEYDYFSTTLHLFFKYNVTETLENAGILASDTKKYPIKDVFGAIESAFKATPKLTCGKKGIIKELRLCFDKDFTPRNCDLTKSCPKSVRLPEFPDLPREIGMVKGAISWMVGA
Homology
BLAST of Tan0012962 vs. ExPASy Swiss-Prot
Match: P42814 (Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 5.2e-73
Identity = 127/236 (53.81%), Postives = 161/236 (68.22%), Query Frame = 0

Query: 27  ALAAEGLTGRPEQREFDYFVLALQWPGTCCRNVTVCCPYNGCCRGADSLTGFTIHGLWPQ 86
           A A + +     QREFDYF L+LQWPGT CR    CC  N CCRG+D+ T FTIHGLWP 
Sbjct: 17  AFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPD 76

Query: 87  YNVKGWPSCCTNATFNENEISTMIEDLDKYWPSYRCGLATSCNNRKVSFWAKEYEKHGTC 146
           YN   WPSCC  + F E EIST+++ L+KYWPS  CG  +SCN  K SFW  E+EKHGTC
Sbjct: 77  YNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTC 136

Query: 147 GSPVIVKEYDYFSTTLHLFFKYNVTETLENAGILASDTKKYPIKDVFGAIESAFKATPKL 206
            SPV   EY+YF TTL+L+ K+NVT+ L  AG +AS+++KYP+  +  AI++AF  TP++
Sbjct: 137 SSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEV 196

Query: 207 TCGKKGIIKELRLCFDKDFTPRNC----DLT--KSCPKSVRLPEFPDLPREIGMVK 257
            C K+  I E+R+CF KDF PR+C    DLT  KSCPK V LPE+  L  E  ++K
Sbjct: 197 VC-KRDAIDEIRICFYKDFKPRDCVGSQDLTSRKSCPKYVSLPEYTPLDGEAMVLK 251

BLAST of Tan0012962 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.3e-28
Identity = 75/227 (33.04%), Postives = 109/227 (48.02%), Query Frame = 0

Query: 28  LAAEGLTGRPEQREFDYFVLALQWPGTCCRNVTVCC-PYNGCCRGADSLTGFTIHGLWPQ 87
           LA + L  +   ++FD+F   LQWPG  C +   CC P  G          F IHGLWP 
Sbjct: 8   LALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTG-----KPAADFGIHGLWPN 67

Query: 88  YNVKGWP-SCCTNATFNENEISTMIEDLDKYWPSYRCGLATSCNNRKVSFWAKEYEKHGT 147
           Y   GWP +C  ++ F++  +S ++ DL + WP+  C      +N  + FW  E+EKHGT
Sbjct: 68  YKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCP-----SNDGMKFWTHEWEKHGT 127

Query: 148 CGSPVIVKEYDYFSTTLHLFFKYNVTETLENAGILASDTKKYPIKDVFGAIESAFKATPK 207
           C    +  ++DYF   L L  K N+   L NAGI   D K Y +KD+   I+      P 
Sbjct: 128 CAESEL-DQHDYFEAGLKLKQKANLLHALTNAGI-KPDDKFYEMKDIENTIKQVVGFAPG 187

Query: 208 LTCGK----KGIIKELRLCFDKDFTP-RNCDLTK--SCPKSVRLPEF 246
           + C K       + ++ LC D   +   NC +     C   V+ P+F
Sbjct: 188 IECNKDSSHNSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQFPKF 222

BLAST of Tan0012962 vs. ExPASy Swiss-Prot
Match: Q7M438 (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 114.4 bits (285), Expect = 2.0e-24
Identity = 71/234 (30.34%), Postives = 114/234 (48.72%), Query Frame = 0

Query: 16  VLAVVAQLQVAALAAEGLTGRPEQR--EFDYFVLALQWPGTCCRNVTVCCPYNGCCRGAD 75
           + A+++ L +A+ A   +T     +  +FD+++   QW  + C + T        C    
Sbjct: 4   IAALLSVLLIASTAQSTVTIYESSKPGDFDFYLFVQQWIYSYCDSQT--------CIQNK 63

Query: 76  SLTGFTIHGLWPQYNVKGWPSCCTNATFNENEISTMIEDLDKYWPSYRCGLATSCNNRKV 135
               FTIHGLWP+ +   +PS C+  +FN N I  + + L+  WP        S      
Sbjct: 64  EREAFTIHGLWPENSDGSYPSFCSGPSFNVNAIQDLEDQLNFDWP--------SLTGPNT 123

Query: 136 SFWAKEYEKHGTCG-SPVIVKEYDYFSTTLHLFFKYNVTETLENAGILASDTKKYPIKDV 195
            FW  E+ KHGTC  +  I   +DYF+T + L+ ++N+T  LE+  I  SD+  Y   D+
Sbjct: 124 DFWTHEFSKHGTCSITGPITDIHDYFATGIKLYTEFNITAALESENIYPSDSNTYKPVDI 183

Query: 196 FGAIESAFKATPKLTCGKKGIIKELRLCFDKD-FTPRNC-DLTK-SCPKSVRLP 244
             AI + F   P + C   G +  + +C DK+  +  +C DL   SC  SV+ P
Sbjct: 184 TNAITTHFGGKPGIQC-SSGQLSTVAVCIDKNSLSIMDCPDLQGWSCSGSVKFP 220

BLAST of Tan0012962 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.0e-24
Identity = 65/213 (30.52%), Postives = 104/213 (48.83%), Query Frame = 0

Query: 16  VLAVVAQLQVAALAAEGLTGRPEQREFDYFVLALQWPGTCCRNVTVCCPYNGCCRGADSL 75
           +LA +  + +  +     +      +FD+F    QWPG+ C     CC  N     AD  
Sbjct: 4   LLASLCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAAD-- 63

Query: 76  TGFTIHGLWPQYNVKGWPS-CCTNATFNENEISTMIEDLDKYWPSYRCGLATSCNNRKVS 135
             F IHGLWP Y    +PS C  +  F+ + IS ++  + K WP+  C   +       +
Sbjct: 64  --FGIHGLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSG-----EA 123

Query: 136 FWAKEYEKHGTCGSPVIVKEYDYFSTTLHLFFKYNVTETLENAGILASDTKKYPIKDVFG 195
           FW  E+EKHGTC S  ++ +++YF T L+L  K N+   L  AGI   D K Y ++ +  
Sbjct: 124 FWEHEWEKHGTC-SESVIDQHEYFQTALNLKQKTNLLGALTKAGI-NPDGKSYSLESIRD 183

Query: 196 AIESAFKATPKLTCGKKGI----IKELRLCFDK 224
           +I+ +   TP + C + G     + ++ LC D+
Sbjct: 184 SIKESIGFTPWVECNRDGSGNSQLYQVYLCVDR 205

BLAST of Tan0012962 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 109.0 bits (271), Expect = 8.3e-23
Identity = 56/171 (32.75%), Postives = 88/171 (51.46%), Query Frame = 0

Query: 40  REFDYFVLALQWPGTCCRNVTVCC-PYNGCCRGADSLTGFTIHGLWPQYNVKGWPS-CCT 99
           ++FD+F    QWPG+ C     CC P  G          F IHGLWP  N   +PS C  
Sbjct: 27  KDFDFFYFVQQWPGSYCDTKQSCCYPTTG-----KPAADFGIHGLWPNNNDGTYPSNCDP 86

Query: 100 NATFNENEISTMIEDLDKYWPSYRCGLATSCNNRKVSFWAKEYEKHGTCGSPVIVKEYDY 159
           N+ +++++IS +I  + + WP+  C   +       +FW+ E+EKHGTC   V+  ++ Y
Sbjct: 87  NSPYDQSQISDLISSMQQNWPTLACPSGSGS-----TFWSHEWEKHGTCAESVLTNQHAY 146

Query: 160 FSTTLHLFFKYNVTETLENAGILASDTKKYPIKDVFGAIESAFKATPKLTC 209
           F   L L  + ++   L+ A I   D + Y + ++  AI+SA   TP + C
Sbjct: 147 FKKALDLKNQIDLLSILQGADI-HPDGESYDLVNIRNAIKSAIGYTPWIQC 186

BLAST of Tan0012962 vs. NCBI nr
Match: XP_022922894.1 (ribonuclease 2-like [Cucurbita moschata])

HSP 1 Score: 393.3 bits (1009), Expect = 1.7e-105
Identity = 187/263 (71.10%), Postives = 211/263 (80.23%), Query Frame = 0

Query: 1   MVSFSFKAAAPVVFPVLAVVAQLQVAALAAEGLTGRPEQREFDYFVLALQWPGTCCRNVT 60
           M SF+FKAAA VV  VLAV  Q Q    AAEGLT   EQREFDYFVLALQWPG+ CRNV+
Sbjct: 1   MASFTFKAAAQVVLMVLAVTLQPQ----AAEGLTVGKEQREFDYFVLALQWPGSSCRNVS 60

Query: 61  VCCPYNGCCRGADSLTGFTIHGLWPQYNVKGWPSCCTNATFNENEISTMIEDLDKYWPSY 120
           +CCP NGCC  A+    FTIHGLWPQY  KGWPSCCTN TFNENE+S++ EDLD+YWPSY
Sbjct: 61  MCCPTNGCCHRAEFPAEFTIHGLWPQYQEKGWPSCCTNETFNENEVSSLFEDLDRYWPSY 120

Query: 121 RCGLATSCNNRKVSFWAKEYEKHGTCGSPVIVKEYDYFSTTLHLFFKYNVTETLENAGIL 180
           RCGL +SC+N+K SFWA +YEKHGTCGSPVI+ +YDYF TTL LFF YNVTE LE+A I+
Sbjct: 121 RCGLVSSCDNKKGSFWAHQYEKHGTCGSPVILHQYDYFFTTLALFFNYNVTEVLEDAEIV 180

Query: 181 ASDTKKYPIKDVFGAIESAFKATPKLTCGKKGIIKELRLCFDKDFTPRNCDLTKSCPKSV 240
           ASDTKKY I+DVF A+ SAFKA PKL+CGK GIIKELRLCFDK+F   +CD TKSCPK V
Sbjct: 181 ASDTKKYGIEDVFEAVRSAFKANPKLSCGKNGIIKELRLCFDKEFKVIDCDATKSCPKFV 240

Query: 241 RLPEFPDLPREIGMVKGAISWMV 264
           RLPEF +LPR   MVK AI  M+
Sbjct: 241 RLPEFHELPRST-MVKRAIPRMI 258

BLAST of Tan0012962 vs. NCBI nr
Match: XP_038876678.1 (ribonuclease 2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 391.0 bits (1003), Expect = 8.4e-105
Identity = 186/260 (71.54%), Postives = 208/260 (80.00%), Query Frame = 0

Query: 1   MVSFSFKAAAPVVF-PVLAVVAQLQVAALAAEGLTGRPEQREFDYFVLALQWPGTCCRNV 60
           M SFS KAAA VVF  VL +V  LQ     AEG TG  EQREFDYFVLALQWP   CRN+
Sbjct: 1   MGSFSLKAAAHVVFVVVLGIVLPLQ----TAEGFTGGKEQREFDYFVLALQWPAALCRNI 60

Query: 61  TVCCPYNGCCRGADSLTGFTIHGLWPQYNVKGWPSCCTNATFNENEISTMIEDLDKYWPS 120
           TVCCP N CCRGADS T FTIHGLWPQY  KGWPSCCTN TFNENEIS++ EDLDKYWPS
Sbjct: 61  TVCCPTNACCRGADSPTEFTIHGLWPQYEGKGWPSCCTNETFNENEISSLFEDLDKYWPS 120

Query: 121 YRCGLATSCNNRKVSFWAKEYEKHGTCGSPVIVKEYDYFSTTLHLFFKYNVTETLENAGI 180
           YRCGL  SC++RK SFWA +YEKHGTCG+PVI++EYDYF TTL LFFKYNVT+ LENA I
Sbjct: 121 YRCGLVGSCDSRKGSFWAHQYEKHGTCGTPVILQEYDYFLTTLTLFFKYNVTKALENAEI 180

Query: 181 LASDTKKYPIKDVFGAIESAFKATPKLTCGKKGIIKELRLCFDKDFTPRNCDLTKSCPKS 240
           +ASD KKYPI+DV  A+  AFKA PKL C K+GIIKE+ LCFDK F  R+CD TKSCPK+
Sbjct: 181 IASDIKKYPIQDVVNAVHLAFKANPKLACAKRGIIKEIYLCFDKQFKLRDCDATKSCPKA 240

Query: 241 VRLPEFPDLPREIGMVKGAI 260
           V+LPEF D P+E+ MVK +I
Sbjct: 241 VKLPEFHD-PQELDMVKRSI 255

BLAST of Tan0012962 vs. NCBI nr
Match: XP_022985160.1 (ribonuclease 2-like [Cucurbita maxima])

HSP 1 Score: 387.1 bits (993), Expect = 1.2e-103
Identity = 184/263 (69.96%), Postives = 208/263 (79.09%), Query Frame = 0

Query: 1   MVSFSFKAAAPVVFPVLAVVAQLQVAALAAEGLTGRPEQREFDYFVLALQWPGTCCRNVT 60
           M SF+FKAAA VVF VLAV    Q    A EGLT   EQREFDYFVLALQWPG+ CRNV+
Sbjct: 1   MASFTFKAAAQVVFMVLAVTLPPQ----AVEGLTVGKEQREFDYFVLALQWPGSSCRNVS 60

Query: 61  VCCPYNGCCRGADSLTGFTIHGLWPQYNVKGWPSCCTNATFNENEISTMIEDLDKYWPSY 120
           +CCP NGCC  A+    FTIHGLWPQY  KGWPSCCTN TFNENE+S++ EDL++YWPSY
Sbjct: 61  MCCPTNGCCHRAELPAEFTIHGLWPQYQDKGWPSCCTNETFNENEVSSLFEDLERYWPSY 120

Query: 121 RCGLATSCNNRKVSFWAKEYEKHGTCGSPVIVKEYDYFSTTLHLFFKYNVTETLENAGIL 180
           RCGL +SC+NRK SFWA +YEKHGTCGSPVI+ +YDYF TTL LFF YNVTE LE A I+
Sbjct: 121 RCGLVSSCSNRKGSFWAHQYEKHGTCGSPVILHQYDYFFTTLALFFNYNVTEALEEADII 180

Query: 181 ASDTKKYPIKDVFGAIESAFKATPKLTCGKKGIIKELRLCFDKDFTPRNCDLTKSCPKSV 240
           ASDTKKY I+DVF A+ SAFKA PK+TCGKKGIIKELRLCFDK+F   +CD TKSCPK V
Sbjct: 181 ASDTKKYGIEDVFRAVRSAFKANPKITCGKKGIIKELRLCFDKEFQLIDCDATKSCPKFV 240

Query: 241 RLPEFPDLPREIGMVKGAISWMV 264
            LPEF +L +   MVK AI  M+
Sbjct: 241 SLPEFHELSKP-AMVKRAIPRMI 258

BLAST of Tan0012962 vs. NCBI nr
Match: XP_038876679.1 (ribonuclease 2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 383.3 bits (983), Expect = 1.7e-102
Identity = 180/248 (72.58%), Postives = 199/248 (80.24%), Query Frame = 0

Query: 1   MVSFSFKAAAPVVF-PVLAVVAQLQVAALAAEGLTGRPEQREFDYFVLALQWPGTCCRNV 60
           M SFS KAAA VVF  VL +V  LQ     AEG TG  EQREFDYFVLALQWP   CRN+
Sbjct: 1   MGSFSLKAAAHVVFVVVLGIVLPLQ----TAEGFTGGKEQREFDYFVLALQWPAALCRNI 60

Query: 61  TVCCPYNGCCRGADSLTGFTIHGLWPQYNVKGWPSCCTNATFNENEISTMIEDLDKYWPS 120
           TVCCP N CCRGADS T FTIHGLWPQY  KGWPSCCTN TFNENEIS++ EDLDKYWPS
Sbjct: 61  TVCCPTNACCRGADSPTEFTIHGLWPQYEGKGWPSCCTNETFNENEISSLFEDLDKYWPS 120

Query: 121 YRCGLATSCNNRKVSFWAKEYEKHGTCGSPVIVKEYDYFSTTLHLFFKYNVTETLENAGI 180
           YRCGL  SC++RK SFWA +YEKHGTCG+PVI++EYDYF TTL LFFKYNVT+ LENA I
Sbjct: 121 YRCGLVGSCDSRKGSFWAHQYEKHGTCGTPVILQEYDYFLTTLTLFFKYNVTKALENAEI 180

Query: 181 LASDTKKYPIKDVFGAIESAFKATPKLTCGKKGIIKELRLCFDKDFTPRNCDLTKSCPKS 240
           +ASD KKYPI+DV  A+  AFKA PKL C K+GIIKE+ LCFDK F  R+CD TKSCPK+
Sbjct: 181 IASDIKKYPIQDVVNAVHLAFKANPKLACAKRGIIKEIYLCFDKQFKLRDCDATKSCPKA 240

Query: 241 VRLPEFPD 248
           V+LPEF D
Sbjct: 241 VKLPEFHD 244

BLAST of Tan0012962 vs. NCBI nr
Match: XP_023551927.1 (ribonuclease 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 376.7 bits (966), Expect = 1.6e-100
Identity = 177/248 (71.37%), Postives = 198/248 (79.84%), Query Frame = 0

Query: 16  VLAVVAQLQVAALAAEGLTGRPEQREFDYFVLALQWPGTCCRNVTVCCPYNGCCRGADSL 75
           VLAV    Q    AAEGLT   EQREFDYFVLALQWPG+ CRNV++CCP NGCC  A+  
Sbjct: 2   VLAVTLPPQ----AAEGLTVGKEQREFDYFVLALQWPGSSCRNVSMCCPTNGCCHRAEFP 61

Query: 76  TGFTIHGLWPQYNVKGWPSCCTNATFNENEISTMIEDLDKYWPSYRCGLATSCNNRKVSF 135
             FTIHGLWPQY  KGWPSCCTN TFN NE+S++ EDLD+YWPSYRCGL +SC+NRK SF
Sbjct: 62  AEFTIHGLWPQYQEKGWPSCCTNETFNGNEVSSLFEDLDRYWPSYRCGLVSSCDNRKGSF 121

Query: 136 WAKEYEKHGTCGSPVIVKEYDYFSTTLHLFFKYNVTETLENAGILASDTKKYPIKDVFGA 195
           WA +YEKHGTCGSPVI+ +YDYF TTL LFF YNVTE LE+A I+ASDTKKY IKDVF A
Sbjct: 122 WAHQYEKHGTCGSPVILHQYDYFFTTLALFFNYNVTEALEDAEIVASDTKKYSIKDVFEA 181

Query: 196 IESAFKATPKLTCGKKGIIKELRLCFDKDFTPRNCDLTKSCPKSVRLPEFPDLPREIGMV 255
           + SAF A PKLTCGKKGIIKELRLCFDK+F   +CD TKSCPK VRLPEF +LP+   MV
Sbjct: 182 VRSAFNANPKLTCGKKGIIKELRLCFDKEFKLIDCDATKSCPKFVRLPEFHELPKST-MV 241

Query: 256 KGAISWMV 264
           K AI  M+
Sbjct: 242 KRAIPRMI 244

BLAST of Tan0012962 vs. ExPASy TrEMBL
Match: A0A6J1E4S1 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111430736 PE=3 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 8.2e-106
Identity = 187/263 (71.10%), Postives = 211/263 (80.23%), Query Frame = 0

Query: 1   MVSFSFKAAAPVVFPVLAVVAQLQVAALAAEGLTGRPEQREFDYFVLALQWPGTCCRNVT 60
           M SF+FKAAA VV  VLAV  Q Q    AAEGLT   EQREFDYFVLALQWPG+ CRNV+
Sbjct: 1   MASFTFKAAAQVVLMVLAVTLQPQ----AAEGLTVGKEQREFDYFVLALQWPGSSCRNVS 60

Query: 61  VCCPYNGCCRGADSLTGFTIHGLWPQYNVKGWPSCCTNATFNENEISTMIEDLDKYWPSY 120
           +CCP NGCC  A+    FTIHGLWPQY  KGWPSCCTN TFNENE+S++ EDLD+YWPSY
Sbjct: 61  MCCPTNGCCHRAEFPAEFTIHGLWPQYQEKGWPSCCTNETFNENEVSSLFEDLDRYWPSY 120

Query: 121 RCGLATSCNNRKVSFWAKEYEKHGTCGSPVIVKEYDYFSTTLHLFFKYNVTETLENAGIL 180
           RCGL +SC+N+K SFWA +YEKHGTCGSPVI+ +YDYF TTL LFF YNVTE LE+A I+
Sbjct: 121 RCGLVSSCDNKKGSFWAHQYEKHGTCGSPVILHQYDYFFTTLALFFNYNVTEVLEDAEIV 180

Query: 181 ASDTKKYPIKDVFGAIESAFKATPKLTCGKKGIIKELRLCFDKDFTPRNCDLTKSCPKSV 240
           ASDTKKY I+DVF A+ SAFKA PKL+CGK GIIKELRLCFDK+F   +CD TKSCPK V
Sbjct: 181 ASDTKKYGIEDVFEAVRSAFKANPKLSCGKNGIIKELRLCFDKEFKVIDCDATKSCPKFV 240

Query: 241 RLPEFPDLPREIGMVKGAISWMV 264
           RLPEF +LPR   MVK AI  M+
Sbjct: 241 RLPEFHELPRST-MVKRAIPRMI 258

BLAST of Tan0012962 vs. ExPASy TrEMBL
Match: A0A6J1JAL4 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111483248 PE=3 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 5.9e-104
Identity = 184/263 (69.96%), Postives = 208/263 (79.09%), Query Frame = 0

Query: 1   MVSFSFKAAAPVVFPVLAVVAQLQVAALAAEGLTGRPEQREFDYFVLALQWPGTCCRNVT 60
           M SF+FKAAA VVF VLAV    Q    A EGLT   EQREFDYFVLALQWPG+ CRNV+
Sbjct: 1   MASFTFKAAAQVVFMVLAVTLPPQ----AVEGLTVGKEQREFDYFVLALQWPGSSCRNVS 60

Query: 61  VCCPYNGCCRGADSLTGFTIHGLWPQYNVKGWPSCCTNATFNENEISTMIEDLDKYWPSY 120
           +CCP NGCC  A+    FTIHGLWPQY  KGWPSCCTN TFNENE+S++ EDL++YWPSY
Sbjct: 61  MCCPTNGCCHRAELPAEFTIHGLWPQYQDKGWPSCCTNETFNENEVSSLFEDLERYWPSY 120

Query: 121 RCGLATSCNNRKVSFWAKEYEKHGTCGSPVIVKEYDYFSTTLHLFFKYNVTETLENAGIL 180
           RCGL +SC+NRK SFWA +YEKHGTCGSPVI+ +YDYF TTL LFF YNVTE LE A I+
Sbjct: 121 RCGLVSSCSNRKGSFWAHQYEKHGTCGSPVILHQYDYFFTTLALFFNYNVTEALEEADII 180

Query: 181 ASDTKKYPIKDVFGAIESAFKATPKLTCGKKGIIKELRLCFDKDFTPRNCDLTKSCPKSV 240
           ASDTKKY I+DVF A+ SAFKA PK+TCGKKGIIKELRLCFDK+F   +CD TKSCPK V
Sbjct: 181 ASDTKKYGIEDVFRAVRSAFKANPKITCGKKGIIKELRLCFDKEFQLIDCDATKSCPKFV 240

Query: 241 RLPEFPDLPREIGMVKGAISWMV 264
            LPEF +L +   MVK AI  M+
Sbjct: 241 SLPEFHELSKP-AMVKRAIPRMI 258

BLAST of Tan0012962 vs. ExPASy TrEMBL
Match: A0A1S3CHU7 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103501145 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 9.4e-94
Identity = 165/248 (66.53%), Postives = 192/248 (77.42%), Query Frame = 0

Query: 12  VVFPVLAVVAQLQVAALAAEGLTGRPEQREFDYFVLALQWPGTCCRNVTVCCPYNGCCRG 71
           VVF V+ V+  +    +   G T   EQREFDYFVLALQWP T CRNVT CCP N C R 
Sbjct: 11  VVFVVVVVIGII----VDGWGFTVGKEQREFDYFVLALQWPSTLCRNVTECCPTNACYRR 70

Query: 72  ADSLTGFTIHGLWPQYNVKGWPSCCTNATFNENEISTMIEDLDKYWPSYRCGLATSCNNR 131
           A S T FTIHGLWP+Y  KGWPSCCTNATFN+ +I ++ ED+DKYWP+YRCGL +SC+NR
Sbjct: 71  AVSATKFTIHGLWPKYEGKGWPSCCTNATFNQTQIGSLFEDVDKYWPTYRCGLVSSCDNR 130

Query: 132 KVSFWAKEYEKHGTCGSPVIVKEYDYFSTTLHLFFKYNVTETLENAGILASDTKKYPIKD 191
           K SFWA +YEKHGTCGSPVI++EYDYF TTL LFFKYNVTE LENA I+ASD +KYPI+D
Sbjct: 131 KGSFWAHQYEKHGTCGSPVILQEYDYFLTTLTLFFKYNVTEALENAEIVASDIRKYPIQD 190

Query: 192 VFGAIESAFKATPKLTCGKKGIIKELRLCFDKDFTPRNCDLTKSCPKSVRLPEFPDLPRE 251
           V  A+ SAFKA PKL C K GIIKE+ LCFDK F  R+CD TKSCPKSV+LP+F   P+E
Sbjct: 191 VLNAVHSAFKANPKLACIKNGIIKEMYLCFDKQFKLRDCDATKSCPKSVKLPKF-HKPQE 250

Query: 252 IGMVKGAI 260
             ++K AI
Sbjct: 251 PDVIKRAI 253

BLAST of Tan0012962 vs. ExPASy TrEMBL
Match: A0A0A0KVR6 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_4G109040 PE=3 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 2.7e-93
Identity = 161/239 (67.36%), Postives = 186/239 (77.82%), Query Frame = 0

Query: 7   KAAAPVVFPVLAVVAQLQVAALAAEGLTGRPEQREFDYFVLALQWPGTCCRNVTVCCPYN 66
           KA +  V  V+ VV  + V  +   G T   EQREFDYF+LALQWP T C NVTVCC  N
Sbjct: 3   KAGSRRVVFVVVVVIGIVVVVVDGGGFTVGKEQREFDYFILALQWPSTSCTNVTVCCTTN 62

Query: 67  GCCRGADSLTGFTIHGLWPQYNVKGWPSCCTNATFNENEISTMIEDLDKYWPSYRCGLAT 126
            CCR A S T FTIHGLWP+Y  KGWPSCCTN TF+E +I ++ ED+DKYWP+YRCGL +
Sbjct: 63  ACCRRAVSPTEFTIHGLWPKYEGKGWPSCCTNETFDETQIRSLFEDVDKYWPTYRCGLVS 122

Query: 127 SCNNRKVSFWAKEYEKHGTCGSPVIVKEYDYFSTTLHLFFKYNVTETLENAGILASDTKK 186
           SC+NRK SFWA +YEKHGTCGSPVI++EYDYF TTL LF+KYNVTE LENA I+ASDTKK
Sbjct: 123 SCDNRKGSFWAHQYEKHGTCGSPVILQEYDYFLTTLTLFYKYNVTEALENAEIVASDTKK 182

Query: 187 YPIKDVFGAIESAFKATPKLTCGKKGIIKELRLCFDKDFTPRNCDLTKSCPKSVRLPEF 246
           YPI+DV  A+ SAFK  PKL C KKGIIKE+ LCFDK F  R+CD TKSCPKSV+LP+F
Sbjct: 183 YPIQDVLNAVHSAFKVNPKLACVKKGIIKEIYLCFDKQFKLRDCDATKSCPKSVKLPKF 241

BLAST of Tan0012962 vs. ExPASy TrEMBL
Match: A0A6J1J670 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111482949 PE=3 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 2.0e-91
Identity = 157/267 (58.80%), Postives = 199/267 (74.53%), Query Frame = 0

Query: 1   MVSFSFKAAAPVVFPVLAVVAQLQVAA--LAAEGLTGRPEQREFDYFVLALQWPGTCCRN 60
           M SF  K+   VV P + ++  LQ+ A  LAAE  +G   Q+EFDYFVLALQWP T C+N
Sbjct: 1   MASFYIKSNPMVVAPAVFLLLALQLVAVVLAAETPSGGGGQKEFDYFVLALQWPATSCKN 60

Query: 61  VTVCCPYNGCCRGADSLTGFTIHGLWPQYNVKGWPSCCTNATFNENEISTMIEDLDKYWP 120
              CCP NGCCRGADS T FTIHGLWPQYN KGWPSCCT+A FN+NEIS + ED++KYWP
Sbjct: 61  TGKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLSEDIEKYWP 120

Query: 121 SYRCGLATSCNNRKVSFWAKEYEKHGTCGSPVIVKEYDYFSTTLHLFFKYNVTETLENAG 180
           +YRCG  ++C+  K SFWA EYEKHGTC +PVIV EYDYF TTL +F KYNVT+ L +AG
Sbjct: 121 TYRCGTTSNCHGTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTLTIFSKYNVTKALSDAG 180

Query: 181 ILASDTKKYPIKDVFGAIESAFKATPKLTCGKKGIIKELRLCFDKDFTPRNCDLTKSCPK 240
            +AS+T+KYP+ DV  A+++A  +TPK++C KKG +KEL +CFDK+F PR+CDL  SCP+
Sbjct: 181 FVASNTEKYPLGDVVDAVKNATNSTPKISCAKKGAVKELWICFDKNFEPRDCDLANSCPE 240

Query: 241 SVRLPEFPDLPREIGMVKGAISWMVGA 266
            ++LP +       G+V+GAI W+  A
Sbjct: 241 FIKLPTY----EPQGVVEGAIPWIADA 263

BLAST of Tan0012962 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 275.8 bits (704), Expect = 3.7e-74
Identity = 127/236 (53.81%), Postives = 161/236 (68.22%), Query Frame = 0

Query: 27  ALAAEGLTGRPEQREFDYFVLALQWPGTCCRNVTVCCPYNGCCRGADSLTGFTIHGLWPQ 86
           A A + +     QREFDYF L+LQWPGT CR    CC  N CCRG+D+ T FTIHGLWP 
Sbjct: 17  AFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPD 76

Query: 87  YNVKGWPSCCTNATFNENEISTMIEDLDKYWPSYRCGLATSCNNRKVSFWAKEYEKHGTC 146
           YN   WPSCC  + F E EIST+++ L+KYWPS  CG  +SCN  K SFW  E+EKHGTC
Sbjct: 77  YNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTC 136

Query: 147 GSPVIVKEYDYFSTTLHLFFKYNVTETLENAGILASDTKKYPIKDVFGAIESAFKATPKL 206
            SPV   EY+YF TTL+L+ K+NVT+ L  AG +AS+++KYP+  +  AI++AF  TP++
Sbjct: 137 SSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEV 196

Query: 207 TCGKKGIIKELRLCFDKDFTPRNC----DLT--KSCPKSVRLPEFPDLPREIGMVK 257
            C K+  I E+R+CF KDF PR+C    DLT  KSCPK V LPE+  L  E  ++K
Sbjct: 197 VC-KRDAIDEIRICFYKDFKPRDCVGSQDLTSRKSCPKYVSLPEYTPLDGEAMVLK 251

BLAST of Tan0012962 vs. TAIR 10
Match: AT2G39780.2 (ribonuclease 2 )

HSP 1 Score: 213.4 bits (542), Expect = 2.2e-55
Identity = 106/236 (44.92%), Postives = 135/236 (57.20%), Query Frame = 0

Query: 27  ALAAEGLTGRPEQREFDYFVLALQWPGTCCRNVTVCCPYNGCCRGADSLTGFTIHGLWPQ 86
           A A + +     QREFDYF L+LQWPGT CR    CC  N CCRG+D+ T FTIHGLWP 
Sbjct: 17  AFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPD 76

Query: 87  YNVKGWPSCCTNATFNENEISTMIEDLDKYWPSYRCGLATSCNNRKVSFWAKEYEKHGTC 146
           YN   WPSCC  + F E EIST+++ L+KYWPS  CG  +SCN  K SFW  E       
Sbjct: 77  YNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHE------- 136

Query: 147 GSPVIVKEYDYFSTTLHLFFKYNVTETLENAGILASDTKKYPIKDVFGAIESAFKATPKL 206
                                    + L  AG +AS+++KYP+  +  AI++AF  TP++
Sbjct: 137 -------------------------DVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEV 196

Query: 207 TCGKKGIIKELRLCFDKDFTPRNC----DLT--KSCPKSVRLPEFPDLPREIGMVK 257
            C K+  I E+R+CF KDF PR+C    DLT  KSCPK V LPE+  L  E  ++K
Sbjct: 197 VC-KRDAIDEIRICFYKDFKPRDCVGSQDLTSRKSCPKYVSLPEYTPLDGEAMVLK 219

BLAST of Tan0012962 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.9e-30
Identity = 75/226 (33.19%), Postives = 110/226 (48.67%), Query Frame = 0

Query: 28  LAAEGLTGRPEQREFDYFVLALQWPGTCCRNVTVCCPYNGCCRGADSLTGFTIHGLWPQY 87
           L  +GL     Q +FD+F   LQWPG  C     CC        AD    F IHGLWP Y
Sbjct: 14  LVFQGLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAAD----FGIHGLWPNY 73

Query: 88  NVKGWPS-CCTNATFNENEISTMIEDLDKYWPSYRCGLATSCNNRKVSFWAKEYEKHGTC 147
           N   WPS C  ++ F+ ++IS ++  L K WP+  C      +N   +FW  E+EKHGTC
Sbjct: 74  NGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCP-----SNEGFNFWEHEWEKHGTC 133

Query: 148 GSPVIVKEYDYFSTTLHLFFKYNVTETLENAGILASDTKKYPIKDVFGAIESAFKATPKL 207
            S  ++ +++YF   L L  K N+ + L+N+GI   D   Y +  +  AI+     TP +
Sbjct: 134 -SESVMDQHEYFENALKLKQKANLLQILKNSGI-NPDDGFYNLDKITNAIKDGIGFTPGI 193

Query: 208 TCGK----KGIIKELRLCFDKDFTP-RNCDLTK--SCPKSVRLPEF 246
            C K       + ++ +C D   T    C +    SCP  ++  +F
Sbjct: 194 ECNKDPERNAQLHQIYICVDTSGTEFIECPVLPRGSCPSQIQFSKF 228

BLAST of Tan0012962 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 128.3 bits (321), Expect = 9.4e-30
Identity = 75/227 (33.04%), Postives = 109/227 (48.02%), Query Frame = 0

Query: 28  LAAEGLTGRPEQREFDYFVLALQWPGTCCRNVTVCC-PYNGCCRGADSLTGFTIHGLWPQ 87
           LA + L  +   ++FD+F   LQWPG  C +   CC P  G          F IHGLWP 
Sbjct: 8   LALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTG-----KPAADFGIHGLWPN 67

Query: 88  YNVKGWP-SCCTNATFNENEISTMIEDLDKYWPSYRCGLATSCNNRKVSFWAKEYEKHGT 147
           Y   GWP +C  ++ F++  +S ++ DL + WP+  C      +N  + FW  E+EKHGT
Sbjct: 68  YKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCP-----SNDGMKFWTHEWEKHGT 127

Query: 148 CGSPVIVKEYDYFSTTLHLFFKYNVTETLENAGILASDTKKYPIKDVFGAIESAFKATPK 207
           C    +  ++DYF   L L  K N+   L NAGI   D K Y +KD+   I+      P 
Sbjct: 128 CAESEL-DQHDYFEAGLKLKQKANLLHALTNAGI-KPDDKFYEMKDIENTIKQVVGFAPG 187

Query: 208 LTCGK----KGIIKELRLCFDKDFTP-RNCDLTK--SCPKSVRLPEF 246
           + C K       + ++ LC D   +   NC +     C   V+ P+F
Sbjct: 188 IECNKDSSHNSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQFPKF 222

BLAST of Tan0012962 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 114.4 bits (285), Expect = 1.4e-25
Identity = 65/213 (30.52%), Postives = 104/213 (48.83%), Query Frame = 0

Query: 16  VLAVVAQLQVAALAAEGLTGRPEQREFDYFVLALQWPGTCCRNVTVCCPYNGCCRGADSL 75
           +LA +  + +  +     +      +FD+F    QWPG+ C     CC  N     AD  
Sbjct: 4   LLASLCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAAD-- 63

Query: 76  TGFTIHGLWPQYNVKGWPS-CCTNATFNENEISTMIEDLDKYWPSYRCGLATSCNNRKVS 135
             F IHGLWP Y    +PS C  +  F+ + IS ++  + K WP+  C   +       +
Sbjct: 64  --FGIHGLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSG-----EA 123

Query: 136 FWAKEYEKHGTCGSPVIVKEYDYFSTTLHLFFKYNVTETLENAGILASDTKKYPIKDVFG 195
           FW  E+EKHGTC S  ++ +++YF T L+L  K N+   L  AGI   D K Y ++ +  
Sbjct: 124 FWEHEWEKHGTC-SESVIDQHEYFQTALNLKQKTNLLGALTKAGI-NPDGKSYSLESIRD 183

Query: 196 AIESAFKATPKLTCGKKGI----IKELRLCFDK 224
           +I+ +   TP + C + G     + ++ LC D+
Sbjct: 184 SIKESIGFTPWVECNRDGSGNSQLYQVYLCVDR 205

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P428145.2e-7353.81Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1[more]
P428151.3e-2833.04Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1[more]
Q7M4382.0e-2430.34Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3[more]
P428132.0e-2430.52Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1[more]
P800228.3e-2332.75Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_022922894.11.7e-10571.10ribonuclease 2-like [Cucurbita moschata][more]
XP_038876678.18.4e-10571.54ribonuclease 2-like isoform X1 [Benincasa hispida][more]
XP_022985160.11.2e-10369.96ribonuclease 2-like [Cucurbita maxima][more]
XP_038876679.11.7e-10272.58ribonuclease 2-like isoform X2 [Benincasa hispida][more]
XP_023551927.11.6e-10071.37ribonuclease 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1E4S18.2e-10671.10Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111430736 PE=3 SV=1[more]
A0A6J1JAL45.9e-10469.96Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111483248 PE=3 SV=1[more]
A0A1S3CHU79.4e-9466.53Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103501145 PE=3 SV=1[more]
A0A0A0KVR62.7e-9367.36Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_4G109040 PE=3 SV=1[more]
A0A6J1J6702.0e-9158.80Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111482949 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G39780.13.7e-7453.81ribonuclease 2 [more]
AT2G39780.22.2e-5544.92ribonuclease 2 [more]
AT1G14220.11.9e-3033.19Ribonuclease T2 family protein [more]
AT1G26820.19.4e-3033.04ribonuclease 3 [more]
AT2G02990.11.4e-2530.52ribonuclease 1 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 42..230
e-value: 3.9E-47
score: 160.8
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 17..239
IPR036430Ribonuclease T2-like superfamilyGENE3D3.90.730.10coord: 38..247
e-value: 6.3E-73
score: 247.2
IPR036430Ribonuclease T2-like superfamilySUPERFAMILY55895Ribonuclease Rh-likecoord: 41..245
NoneNo IPR availablePANTHERPTHR11240:SF66OS01G0897300 PROTEINcoord: 17..239
IPR018188Ribonuclease T2, His active site 1PROSITEPS00530RNASE_T2_1coord: 78..85
IPR033697Ribonuclease T2, eukaryoticCDDcd01061RNase_T2_eukcoord: 42..239
e-value: 2.04955E-62
score: 192.932

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0012962.1Tan0012962.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006401 RNA catabolic process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0005576 extracellular region
cellular_component GO:0016020 membrane
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding