Tan0012961 (gene) Snake gourd v1

BLAST of Tan0012961 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 3.4e-130
Identity = 230/331 (69.49%), Postives = 276/331 (83.38%), Query Frame = 0

Query: 7   ILSLCLLGLLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYDPNRASL 66
           +L   ++G L    H T A IGVC G +G++LPPQ EV+ L+   NI RMRLYDPN A+L
Sbjct: 21  LLGQLVVGSLATKQH-TGAPIGVCNGMVGDDLPPQAEVVALYKTNNIPRMRLYDPNPAAL 80

Query: 67  DALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFPNVRFKYIAVGNEVQPSSPT 126
           +ALRGSNI+L+LG+PN +LQ I  SQA ANAW+QNNVRN+ NV+FKYIAVGNEV+PS   
Sbjct: 81  EALRGSNIKLLLGVPNENLQYIALSQANANAWVQNNVRNYANVKFKYIAVGNEVKPSDSF 140

Query: 127 AAFVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSLLNPVIRF 186
           A F+VPAMRNIQ A+S AGLA +IKVSTAIDTG++G +FPPS GSF+++  +LL P+IRF
Sbjct: 141 AQFLVPAMRNIQEAISLAGLAKKIKVSTAIDTGVLGETFPPSIGSFKSEYNALLYPIIRF 200

Query: 187 LIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAMLDAVYAA 246
           L+ ++SPLLVNLYPYF+Y GN   IRLDYALFTAPSVVV DG FGYRNLFDAMLD VYAA
Sbjct: 201 LVSHQSPLLVNLYPYFAYSGNTQDIRLDYALFTAPSVVVQDGNFGYRNLFDAMLDGVYAA 260

Query: 247 LEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRPIETYIFA 306
           LEK GGG+L++VISE+GWPSA GTAT+IDNART+ +NLI+HVK GTPRRPGRPIETYIFA
Sbjct: 261 LEKAGGGSLKVVISETGWPSAAGTATTIDNARTFISNLIQHVKEGTPRRPGRPIETYIFA 320

Query: 307 MFNENQKNPEFEKHFGLFSPNKQLKYPVNFN 338
           MF+EN+K PE EKH+GLFSP KQ KY ++FN
Sbjct: 321 MFDENRKTPELEKHWGLFSPTKQPKYQISFN 350

BLAST of Tan0012961 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 435.6 bits (1119), Expect = 4.9e-121
Identity = 214/333 (64.26%), Postives = 266/333 (79.88%), Query Frame = 0

Query: 9   SLCLLGLLLPNLHLTSAQ-IGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYDPNRASLD 68
           ++ LLGLL+ +  +  AQ +GVCYG +GNNLPP  +V+ L+  KNI+RMRLYDPN+A+L 
Sbjct: 14  AIILLGLLVSSTEIVGAQSVGVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAALQ 73

Query: 69  ALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNF-PNVRFKYIAVGNEVQPSSPT 128
           ALRGSNIE+MLG+PNSDLQ I ++ + AN W+Q NVRNF P V+F+YIAVGNEV P + T
Sbjct: 74  ALRGSNIEVMLGVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVTGT 133

Query: 129 AA---FVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSLLNPV 188
           ++   +++PAMRNI+NA+S+AGL   IKVS+++D  LIG SFPPS GSFRND  S ++P+
Sbjct: 134 SSLTRYLLPAMRNIRNAISSAGLQNNIKVSSSVDMTLIGNSFPPSQGSFRNDVRSFIDPI 193

Query: 189 IRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAMLDAV 248
           I F+    SPLLVN+YPYFSY GNP  I L YALFTAP+VVV DG  GYRNLFDAM DAV
Sbjct: 194 IGFVRRINSPLLVNIYPYFSYAGNPRDISLPYALFTAPNVVVQDGSLGYRNLFDAMSDAV 253

Query: 249 YAALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRPIETY 308
           YAAL + GGG++EIV+SESGWPSAG  A + +NA TY  NLI+HVK G+PRRP + IETY
Sbjct: 254 YAALSRAGGGSIEIVVSESGWPSAGAFAATTNNAATYYKNLIQHVKRGSPRRPNKVIETY 313

Query: 309 IFAMFNENQKNPEFEKHFGLFSPNKQLKYPVNF 337
           +FAMF+EN KNPE EKHFGLFSPNKQ KYP++F
Sbjct: 314 LFAMFDENNKNPELEKHFGLFSPNKQPKYPLSF 346

BLAST of Tan0012961 vs. ExPASy Swiss-Prot
Match: Q03773 (Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 1.3e-118
Identity = 207/326 (63.50%), Postives = 261/326 (80.06%), Query Frame = 0

Query: 15  LLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYDPNRASLDALRGSNI 74
           LL+     T AQ GVCYG++GNNLP  +EV+ L+NQ NI+RMR+Y P+   L+ALRGSNI
Sbjct: 22  LLITYTGTTDAQSGVCYGRLGNNLPTPQEVVALYNQANIRRMRIYGPSPEVLEALRGSNI 81

Query: 75  ELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFP-NVRFKYIAVGNEVQPSSPTAAFVVPA 134
           EL+L +PN +L+ + SSQ  AN W+Q+N++N+  NVRF+Y++VGNEV+P    A F+VPA
Sbjct: 82  ELLLDIPNDNLRNLASSQDNANKWVQDNIKNYANNVRFRYVSVGNEVKPEHSFAQFLVPA 141

Query: 135 MRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAM-SLLNPVIRFLIDNRS 194
           + NIQ A+SNAGL  ++KVSTAIDTG +  SFPPS GSF++D   + L+ VIRFL++N +
Sbjct: 142 LENIQRAISNAGLGNQVKVSTAIDTGALAESFPPSKGSFKSDYRGAYLDGVIRFLVNNNA 201

Query: 195 PLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAMLDAVYAALEKVGG 254
           PL+VN+Y YF+Y  NP  I LDYALF +PSVVV DG  GYRNLFDA +DAVYAALEK GG
Sbjct: 202 PLMVNVYSYFAYTANPKDISLDYALFRSPSVVVQDGSLGYRNLFDASVDAVYAALEKAGG 261

Query: 255 GNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRPIETYIFAMFNENQ 314
           G+L IV+SESGWPS+GGTATS+DNARTYN NL+R+VK GTP+RPG P+ETY+FAMF+ENQ
Sbjct: 262 GSLNIVVSESGWPSSGGTATSLDNARTYNTNLVRNVKQGTPKRPGAPLETYVFAMFDENQ 321

Query: 315 KNPEFEKHFGLFSP-NKQLKYPVNFN 338
           K PEFEK +GLFSP  KQ KY +NFN
Sbjct: 322 KQPEFEKFWGLFSPITKQPKYSINFN 347

BLAST of Tan0012961 vs. ExPASy Swiss-Prot
Match: P36401 (Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 4.5e-114
Identity = 207/336 (61.61%), Postives = 262/336 (77.98%), Query Frame = 0

Query: 7   ILSLCLLGLL-LPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYDPNRAS 66
           I++L LL +L L  L  T AQ GVCYG+ GN LP   +V++L N+ NI+RMR+YDP++ +
Sbjct: 5   IVTLLLLSVLTLATLDFTGAQAGVCYGRQGNGLPSPADVVSLCNRNNIRRMRIYDPDQPT 64

Query: 67  LDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFPNVRFKYIAVGNEVQPSSP 126
           L+ALRGSNIELMLG+PN DL+ + +SQA A+ W+QNNVRN+ NV+F+YIAVGNEV P + 
Sbjct: 65  LEALRGSNIELMLGVPNPDLENVAASQANADTWVQNNVRNYGNVKFRYIAVGNEVSPLNE 124

Query: 127 TAAFV---VPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSLLNP 186
            + +V   + AMRNIQ A+S AGL  +IKVSTAI+TGL   + PPS+G F++D    + P
Sbjct: 125 NSKYVPVLLNAMRNIQTAISGAGLGNQIKVSTAIETGLTTDTSPPSNGRFKDDVRQFIEP 184

Query: 187 VIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAMLDA 246
           +I FL+ NR+PLLVNLYPYF+ I N   I+L+YALFT+  VVV D   GYRNLFDA+LDA
Sbjct: 185 IINFLVTNRAPLLVNLYPYFA-IANNADIKLEYALFTSSEVVVNDNGRGYRNLFDAILDA 244

Query: 247 VYAALEKVGGGNLEIVISESGWPSAG-GTATSIDNARTYNNNLIRHVKGGTPRRPGRPIE 306
            Y+ALEK  G +LEIV+SESGWPSAG G  TSIDNARTYNNNLI HVKGG+P+RP  PIE
Sbjct: 245 TYSALEKASGSSLEIVVSESGWPSAGAGQLTSIDNARTYNNNLISHVKGGSPKRPSGPIE 304

Query: 307 TYIFAMFNENQKNPEFEKHFGLFSPNKQLKYPVNFN 338
           TY+FA+F+E+QK+PE EKHFGLFS N Q KY ++FN
Sbjct: 305 TYVFALFDEDQKDPEIEKHFGLFSANMQPKYQISFN 339

BLAST of Tan0012961 vs. ExPASy Swiss-Prot
Match: Q01413 (Glucan endo-1,3-beta-glucosidase B OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 5.9e-114
Identity = 208/335 (62.09%), Postives = 255/335 (76.12%), Query Frame = 0

Query: 7   ILSLCLLGLLL-PNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYDPNRAS 66
           I  + LLGLL+  N+H+T AQIGVCYG MGNNLP   EVI L+  +NI+R+RLYDPN  +
Sbjct: 6   IAIIVLLGLLVATNIHITEAQIGVCYGMMGNNLPSHSEVIQLYKSRNIRRLRLYDPNHGA 65

Query: 67  LDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNF-PNVRFKYIAVGNEVQP-- 126
           L+ALRGSNIE++LGLPN D++ I S    A  W+Q NVR+F P+V+ KYIAVGNE+ P  
Sbjct: 66  LNALRGSNIEVILGLPNVDVKHISSGMEHARWWVQKNVRDFWPHVKIKYIAVGNEISPVT 125

Query: 127 -SSPTAAFVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSLLN 186
            +S  A F VPA+ NI  A+  AGL   IKVST++D  LIG S+PPS GSFRND     +
Sbjct: 126 GTSNLAPFQVPALVNIYKAIGEAGLGNDIKVSTSVDMTLIGNSYPPSQGSFRNDVRWFTD 185

Query: 187 PVIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAMLD 246
           P++ FL D R+PLLVN+YPYFSY GNP  I L YALFTAP+VVV DG   YRNLFDAMLD
Sbjct: 186 PIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYALFTAPNVVVQDGSRQYRNLFDAMLD 245

Query: 247 AVYAALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRPIE 306
           +VYAA+++ GGG++ IV+SESGWPSAG    + +NA+TY  NLI+H K G+PR+PG PIE
Sbjct: 246 SVYAAMDRTGGGSVGIVVSESGWPSAGAFGATHENAQTYLRNLIQHAKEGSPRKPG-PIE 305

Query: 307 TYIFAMFNENQKNPEFEKHFGLFSPNKQLKYPVNF 337
           TYIFAMF+EN KNPE EKHFG+FSPNKQ KY +NF
Sbjct: 306 TYIFAMFDENNKNPELEKHFGMFSPNKQPKYNLNF 339

BLAST of Tan0012961 vs. NCBI nr
Match: XP_038876764.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida])

HSP 1 Score: 560.8 bits (1444), Expect = 7.8e-156
Identity = 278/337 (82.49%), Postives = 303/337 (89.91%), Query Frame = 0

Query: 1   MANFYSILSLCLLGLLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYD 60
           MANFYSI+SL LLG LLPNLHLTSAQIGVCYGQ+GNNLP QREV+ LFNQ+NI+RMRLYD
Sbjct: 1   MANFYSIISLFLLGFLLPNLHLTSAQIGVCYGQLGNNLPSQREVVNLFNQRNIRRMRLYD 60

Query: 61  PNRASLDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFPNVRFKYIAVGNEV 120
           PNRASLDALRGSNIELMLGLPNSDLQRI SSQ+EANAW+QNNV++F NVRFKYIAVGNEV
Sbjct: 61  PNRASLDALRGSNIELMLGLPNSDLQRIASSQSEANAWVQNNVKSFGNVRFKYIAVGNEV 120

Query: 121 QPSSPTAAFVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSLL 180
           QPSS  A FVVPAM+NIQ ALSN GLA +IKVSTAIDTGL+GVSFPPSSG+F N AM LL
Sbjct: 121 QPSSSAAQFVVPAMQNIQTALSNGGLANQIKVSTAIDTGLLGVSFPPSSGAFNNQAMPLL 180

Query: 181 NPVIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAML 240
           NP+IRFL  NRSPLLVNLYP+FSYI NP  IRLDYALFTA S VVTDG   Y+NLFDA++
Sbjct: 181 NPIIRFLNANRSPLLVNLYPFFSYISNPRDIRLDYALFTAKSTVVTDGQLRYQNLFDAIV 240

Query: 241 DAVYAALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRPI 300
           DA+YAALEK GG +LEIV+SESGWPSAGGTA S +NARTYNNNLI+HVK GTPRRP RPI
Sbjct: 241 DAIYAALEKAGGSSLEIVVSESGWPSAGGTAASTENARTYNNNLIQHVKRGTPRRPQRPI 300

Query: 301 ETYIFAMFNENQKNPEFEKHFGLFSPNKQLKYPVNFN 338
           ETYIFAMF+ENQK+PEFEKHFGLF PNKQ KY VNFN
Sbjct: 301 ETYIFAMFDENQKSPEFEKHFGLFFPNKQPKYAVNFN 337

BLAST of Tan0012961 vs. NCBI nr
Match: XP_008443726.1 (PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo])

HSP 1 Score: 557.4 bits (1435), Expect = 8.6e-155
Identity = 278/339 (82.01%), Postives = 304/339 (89.68%), Query Frame = 0

Query: 1   MANF-YSILSLCLLGLLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLY 60
           MANF ++++ LCLLGLL+ NLHLTSAQIGVCYGQMGNNLPPQREV+TL+NQ NIKRMRLY
Sbjct: 1   MANFSWTMICLCLLGLLVQNLHLTSAQIGVCYGQMGNNLPPQREVVTLYNQNNIKRMRLY 60

Query: 61  DPNRASLDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFPNVRFKYIAVGNE 120
           DPNR SLDALRGS IELMLGLPN DLQRI SSQAEANAW+QNN++N+ NVRFKYIAVGNE
Sbjct: 61  DPNRGSLDALRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIAVGNE 120

Query: 121 VQPSSPTAAFVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSL 180
           VQPSS  A+FVVPAMRNIQ AL+NAGL  RIKVSTAIDT ++ +S+PPSSG+ +N+ M  
Sbjct: 121 VQPSSSAASFVVPAMRNIQTALNNAGLVNRIKVSTAIDTSILAMSYPPSSGTIKNEDMPF 180

Query: 181 LNPVIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAM 240
           ++ VIRFL DN SPLLVNLYPYFSYIGNP  IRLDYALFTAPS VV DG F Y+NLFDA+
Sbjct: 181 MSSVIRFLKDNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNLFDAI 240

Query: 241 LDAVYAALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRP-GR 300
           LDAVYAALEKVGGGNLEIVISESGWPSAGGTA SIDNARTY NNLI+HVK GTPRRP GR
Sbjct: 241 LDAVYAALEKVGGGNLEIVISESGWPSAGGTAASIDNARTYINNLIQHVKRGTPRRPGGR 300

Query: 301 PIETYIFAMFNENQKNPEFEKHFGLFSPNKQLKYPVNFN 338
           PIETYIFAMF+ENQKNPEFEKHFGLF PNKQ KYPVNFN
Sbjct: 301 PIETYIFAMFDENQKNPEFEKHFGLFFPNKQSKYPVNFN 339

BLAST of Tan0012961 vs. NCBI nr
Match: XP_022138965.1 (LOW QUALITY PROTEIN: glucan endo-1,3-beta-glucosidase, basic isoform-like [Momordica charantia])

HSP 1 Score: 556.6 bits (1433), Expect = 1.5e-154
Identity = 275/338 (81.36%), Postives = 301/338 (89.05%), Query Frame = 0

Query: 1   MANFYSILSLCLLGLLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYD 60
           M N YSI+SLCLL  LLPNLHLTSAQIGVCYGQ+GNNLPPQREV+ L+NQ NI+RMRLYD
Sbjct: 1   MDNLYSIISLCLLAFLLPNLHLTSAQIGVCYGQIGNNLPPQREVVALYNQNNIRRMRLYD 60

Query: 61  PNRASLDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFPNVRFKYIAVGNEV 120
           PNRASLDAL+ SNIEL+LGLPN DLQRI SSQ EANAW+QNNVRNF NVRFKYIAVGNEV
Sbjct: 61  PNRASLDALKXSNIELILGLPNPDLQRIASSQGEANAWVQNNVRNFANVRFKYIAVGNEV 120

Query: 121 QPSSPTAA-FVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSL 180
             S P AA FV PAM+NIQNA+S AGL  +IKVSTAIDT +IGVSFPPS+G+F+ D    
Sbjct: 121 LSSDPGAARFVGPAMQNIQNAISAAGLGGQIKVSTAIDTRVIGVSFPPSAGAFKTDVRGF 180

Query: 181 LNPVIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAM 240
           ++PVIRFL+ NRSPLL+NLYPYFSYIGN   IRLDYALFTAPSVVVTDG F Y+NLFDA+
Sbjct: 181 MDPVIRFLVANRSPLLLNLYPYFSYIGNTRDIRLDYALFTAPSVVVTDGQFRYQNLFDAI 240

Query: 241 LDAVYAALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRP 300
           LD+ YAALEK GGGNLEIVISESGWPSAGGTATSIDNARTYNNNLI+HVK GTPRRPGRP
Sbjct: 241 LDSFYAALEKAGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIQHVKRGTPRRPGRP 300

Query: 301 IETYIFAMFNENQKNPEFEKHFGLFSPNKQLKYPVNFN 338
           IETYIF MF+ENQK+PEFEKHFGLFSPNKQ KYP+NFN
Sbjct: 301 IETYIFGMFDENQKSPEFEKHFGLFSPNKQRKYPINFN 338

BLAST of Tan0012961 vs. NCBI nr
Match: XP_022960641.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita moschata])

HSP 1 Score: 553.1 bits (1424), Expect = 1.6e-153
Identity = 269/337 (79.82%), Postives = 301/337 (89.32%), Query Frame = 0

Query: 1   MANFYSILSLCLLGLLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYD 60
           MAN YSI+SLCLLGLL+P+LHLTSAQIGVCYGQMGNNLPP REV+ L+NQ NI+RMRLY 
Sbjct: 1   MANSYSIVSLCLLGLLMPSLHLTSAQIGVCYGQMGNNLPPSREVVALYNQNNIRRMRLYG 60

Query: 61  PNRASLDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFPNVRFKYIAVGNEV 120
           PNR SL AL GSNIEL+L +PN DL +I SSQAEAN W+QNN+R+ PNVRFKYIAVGNEV
Sbjct: 61  PNRDSLGALSGSNIELILDVPNPDLPKIASSQAEANTWVQNNIRSLPNVRFKYIAVGNEV 120

Query: 121 QPSSPTAAFVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSLL 180
           Q SS  A FVVPAMRNIQ AL++ GL  RIKVSTAIDTG++G SFPPS+G+F+++AM LL
Sbjct: 121 QSSSSNAQFVVPAMRNIQTALASVGLGGRIKVSTAIDTGVLGTSFPPSNGAFKSEAMPLL 180

Query: 181 NPVIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAML 240
           NP+IRFLI+N SPLLVNLYPYFSYIGN   IRLDYALFTAPS VVTDG F Y+NLFDA+L
Sbjct: 181 NPIIRFLIENGSPLLVNLYPYFSYIGNMRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240

Query: 241 DAVYAALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRPI 300
           DA YAALEK GG N+EIV+SESGWP+AGGTATSIDNARTY NNLI+HVK GTPRRPG+PI
Sbjct: 241 DATYAALEKAGGRNVEIVVSESGWPTAGGTATSIDNARTYINNLIQHVKRGTPRRPGKPI 300

Query: 301 ETYIFAMFNENQKNPEFEKHFGLFSPNKQLKYPVNFN 338
           ETYIFAMF+ENQKNPEFEKHFGLFSPNKQLKYP+NFN
Sbjct: 301 ETYIFAMFDENQKNPEFEKHFGLFSPNKQLKYPINFN 337

BLAST of Tan0012961 vs. NCBI nr
Match: XP_022987104.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita maxima])

HSP 1 Score: 552.7 bits (1423), Expect = 2.1e-153
Identity = 269/337 (79.82%), Postives = 300/337 (89.02%), Query Frame = 0

Query: 1   MANFYSILSLCLLGLLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYD 60
           MAN YSI+SLCLLGLL+  LHLTSAQIGVCYGQMGNNLPP REV+ L+NQ NI+RMRLY 
Sbjct: 1   MANSYSIISLCLLGLLMSTLHLTSAQIGVCYGQMGNNLPPSREVVALYNQNNIRRMRLYG 60

Query: 61  PNRASLDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFPNVRFKYIAVGNEV 120
           PNR SL AL GSNIEL+L +PN DLQRI SSQAEAN W+QNN+R+FPNVRFKYI+VGNEV
Sbjct: 61  PNRDSLGALSGSNIELILDVPNPDLQRIASSQAEANTWVQNNIRSFPNVRFKYISVGNEV 120

Query: 121 QPSSPTAAFVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSLL 180
           QPSS  A FVVPAMRNIQ AL+N GL++RIKVST+IDTG+IG SFPPS+G+F+++AM LL
Sbjct: 121 QPSSSNAQFVVPAMRNIQTALANVGLSSRIKVSTSIDTGVIGTSFPPSNGAFKSEAMPLL 180

Query: 181 NPVIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAML 240
           NP+IRFL +N SPLLVNLYPYFSYIGN   IRLDYALFTAPS VVTDG F Y+NLFDA+L
Sbjct: 181 NPIIRFLNENSSPLLVNLYPYFSYIGNMRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240

Query: 241 DAVYAALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRPI 300
           DA YAALEK GG NLEIV+SESGWP+AGG ATSI NARTY NNLI+HVK GTPRRPGRPI
Sbjct: 241 DATYAALEKAGGRNLEIVVSESGWPTAGGAATSISNARTYINNLIQHVKRGTPRRPGRPI 300

Query: 301 ETYIFAMFNENQKNPEFEKHFGLFSPNKQLKYPVNFN 338
           E YIFAMF+ENQKNPEFEKHFGLF+PNKQLKYP+NFN
Sbjct: 301 EAYIFAMFDENQKNPEFEKHFGLFTPNKQLKYPINFN 337

BLAST of Tan0012961 vs. ExPASy TrEMBL
Match: A0A1S3B9H8 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487244 PE=3 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 4.2e-155
Identity = 278/339 (82.01%), Postives = 304/339 (89.68%), Query Frame = 0

Query: 1   MANF-YSILSLCLLGLLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLY 60
           MANF ++++ LCLLGLL+ NLHLTSAQIGVCYGQMGNNLPPQREV+TL+NQ NIKRMRLY
Sbjct: 1   MANFSWTMICLCLLGLLVQNLHLTSAQIGVCYGQMGNNLPPQREVVTLYNQNNIKRMRLY 60

Query: 61  DPNRASLDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFPNVRFKYIAVGNE 120
           DPNR SLDALRGS IELMLGLPN DLQRI SSQAEANAW+QNN++N+ NVRFKYIAVGNE
Sbjct: 61  DPNRGSLDALRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIAVGNE 120

Query: 121 VQPSSPTAAFVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSL 180
           VQPSS  A+FVVPAMRNIQ AL+NAGL  RIKVSTAIDT ++ +S+PPSSG+ +N+ M  
Sbjct: 121 VQPSSSAASFVVPAMRNIQTALNNAGLVNRIKVSTAIDTSILAMSYPPSSGTIKNEDMPF 180

Query: 181 LNPVIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAM 240
           ++ VIRFL DN SPLLVNLYPYFSYIGNP  IRLDYALFTAPS VV DG F Y+NLFDA+
Sbjct: 181 MSSVIRFLKDNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNLFDAI 240

Query: 241 LDAVYAALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRP-GR 300
           LDAVYAALEKVGGGNLEIVISESGWPSAGGTA SIDNARTY NNLI+HVK GTPRRP GR
Sbjct: 241 LDAVYAALEKVGGGNLEIVISESGWPSAGGTAASIDNARTYINNLIQHVKRGTPRRPGGR 300

Query: 301 PIETYIFAMFNENQKNPEFEKHFGLFSPNKQLKYPVNFN 338
           PIETYIFAMF+ENQKNPEFEKHFGLF PNKQ KYPVNFN
Sbjct: 301 PIETYIFAMFDENQKNPEFEKHFGLFFPNKQSKYPVNFN 339

BLAST of Tan0012961 vs. ExPASy TrEMBL
Match: A0A6J1CAZ7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010014 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 7.1e-155
Identity = 275/338 (81.36%), Postives = 301/338 (89.05%), Query Frame = 0

Query: 1   MANFYSILSLCLLGLLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYD 60
           M N YSI+SLCLL  LLPNLHLTSAQIGVCYGQ+GNNLPPQREV+ L+NQ NI+RMRLYD
Sbjct: 1   MDNLYSIISLCLLAFLLPNLHLTSAQIGVCYGQIGNNLPPQREVVALYNQNNIRRMRLYD 60

Query: 61  PNRASLDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFPNVRFKYIAVGNEV 120
           PNRASLDAL+ SNIEL+LGLPN DLQRI SSQ EANAW+QNNVRNF NVRFKYIAVGNEV
Sbjct: 61  PNRASLDALKXSNIELILGLPNPDLQRIASSQGEANAWVQNNVRNFANVRFKYIAVGNEV 120

Query: 121 QPSSPTAA-FVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSL 180
             S P AA FV PAM+NIQNA+S AGL  +IKVSTAIDT +IGVSFPPS+G+F+ D    
Sbjct: 121 LSSDPGAARFVGPAMQNIQNAISAAGLGGQIKVSTAIDTRVIGVSFPPSAGAFKTDVRGF 180

Query: 181 LNPVIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAM 240
           ++PVIRFL+ NRSPLL+NLYPYFSYIGN   IRLDYALFTAPSVVVTDG F Y+NLFDA+
Sbjct: 181 MDPVIRFLVANRSPLLLNLYPYFSYIGNTRDIRLDYALFTAPSVVVTDGQFRYQNLFDAI 240

Query: 241 LDAVYAALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRP 300
           LD+ YAALEK GGGNLEIVISESGWPSAGGTATSIDNARTYNNNLI+HVK GTPRRPGRP
Sbjct: 241 LDSFYAALEKAGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIQHVKRGTPRRPGRP 300

Query: 301 IETYIFAMFNENQKNPEFEKHFGLFSPNKQLKYPVNFN 338
           IETYIF MF+ENQK+PEFEKHFGLFSPNKQ KYP+NFN
Sbjct: 301 IETYIFGMFDENQKSPEFEKHFGLFSPNKQRKYPINFN 338

BLAST of Tan0012961 vs. ExPASy TrEMBL
Match: A0A6J1H865 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461371 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 7.8e-154
Identity = 269/337 (79.82%), Postives = 301/337 (89.32%), Query Frame = 0

Query: 1   MANFYSILSLCLLGLLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYD 60
           MAN YSI+SLCLLGLL+P+LHLTSAQIGVCYGQMGNNLPP REV+ L+NQ NI+RMRLY 
Sbjct: 1   MANSYSIVSLCLLGLLMPSLHLTSAQIGVCYGQMGNNLPPSREVVALYNQNNIRRMRLYG 60

Query: 61  PNRASLDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFPNVRFKYIAVGNEV 120
           PNR SL AL GSNIEL+L +PN DL +I SSQAEAN W+QNN+R+ PNVRFKYIAVGNEV
Sbjct: 61  PNRDSLGALSGSNIELILDVPNPDLPKIASSQAEANTWVQNNIRSLPNVRFKYIAVGNEV 120

Query: 121 QPSSPTAAFVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSLL 180
           Q SS  A FVVPAMRNIQ AL++ GL  RIKVSTAIDTG++G SFPPS+G+F+++AM LL
Sbjct: 121 QSSSSNAQFVVPAMRNIQTALASVGLGGRIKVSTAIDTGVLGTSFPPSNGAFKSEAMPLL 180

Query: 181 NPVIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAML 240
           NP+IRFLI+N SPLLVNLYPYFSYIGN   IRLDYALFTAPS VVTDG F Y+NLFDA+L
Sbjct: 181 NPIIRFLIENGSPLLVNLYPYFSYIGNMRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240

Query: 241 DAVYAALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRPI 300
           DA YAALEK GG N+EIV+SESGWP+AGGTATSIDNARTY NNLI+HVK GTPRRPG+PI
Sbjct: 241 DATYAALEKAGGRNVEIVVSESGWPTAGGTATSIDNARTYINNLIQHVKRGTPRRPGKPI 300

Query: 301 ETYIFAMFNENQKNPEFEKHFGLFSPNKQLKYPVNFN 338
           ETYIFAMF+ENQKNPEFEKHFGLFSPNKQLKYP+NFN
Sbjct: 301 ETYIFAMFDENQKNPEFEKHFGLFSPNKQLKYPINFN 337

BLAST of Tan0012961 vs. ExPASy TrEMBL
Match: A0A6J1JHX8 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484757 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 1.0e-153
Identity = 269/337 (79.82%), Postives = 300/337 (89.02%), Query Frame = 0

Query: 1   MANFYSILSLCLLGLLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYD 60
           MAN YSI+SLCLLGLL+  LHLTSAQIGVCYGQMGNNLPP REV+ L+NQ NI+RMRLY 
Sbjct: 1   MANSYSIISLCLLGLLMSTLHLTSAQIGVCYGQMGNNLPPSREVVALYNQNNIRRMRLYG 60

Query: 61  PNRASLDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFPNVRFKYIAVGNEV 120
           PNR SL AL GSNIEL+L +PN DLQRI SSQAEAN W+QNN+R+FPNVRFKYI+VGNEV
Sbjct: 61  PNRDSLGALSGSNIELILDVPNPDLQRIASSQAEANTWVQNNIRSFPNVRFKYISVGNEV 120

Query: 121 QPSSPTAAFVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSLL 180
           QPSS  A FVVPAMRNIQ AL+N GL++RIKVST+IDTG+IG SFPPS+G+F+++AM LL
Sbjct: 121 QPSSSNAQFVVPAMRNIQTALANVGLSSRIKVSTSIDTGVIGTSFPPSNGAFKSEAMPLL 180

Query: 181 NPVIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAML 240
           NP+IRFL +N SPLLVNLYPYFSYIGN   IRLDYALFTAPS VVTDG F Y+NLFDA+L
Sbjct: 181 NPIIRFLNENSSPLLVNLYPYFSYIGNMRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240

Query: 241 DAVYAALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRPI 300
           DA YAALEK GG NLEIV+SESGWP+AGG ATSI NARTY NNLI+HVK GTPRRPGRPI
Sbjct: 241 DATYAALEKAGGRNLEIVVSESGWPTAGGAATSISNARTYINNLIQHVKRGTPRRPGRPI 300

Query: 301 ETYIFAMFNENQKNPEFEKHFGLFSPNKQLKYPVNFN 338
           E YIFAMF+ENQKNPEFEKHFGLF+PNKQLKYP+NFN
Sbjct: 301 EAYIFAMFDENQKNPEFEKHFGLFTPNKQLKYPINFN 337

BLAST of Tan0012961 vs. ExPASy TrEMBL
Match: A0A0A0LXW2 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G660200 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.9e-153
Identity = 270/337 (80.12%), Postives = 302/337 (89.61%), Query Frame = 0

Query: 1   MANFYSILSLCLLGLLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYD 60
           MANF  I+SLCLLGLL+PNLHLT+AQIGVCYGQMGNNLPPQ EV+TLFNQ NIKRMRLYD
Sbjct: 1   MANFSPIISLCLLGLLVPNLHLTNAQIGVCYGQMGNNLPPQTEVVTLFNQNNIKRMRLYD 60

Query: 61  PNRASLDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFPNVRFKYIAVGNEV 120
           PNR SLDALRGS IELMLG+PNSDLQRI SSQ EANAW+QNNV+N+PNVRF+YIAVGNEV
Sbjct: 61  PNRGSLDALRGSPIELMLGVPNSDLQRIASSQTEANAWVQNNVKNYPNVRFRYIAVGNEV 120

Query: 121 QPSSPTAAFVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSLL 180
           QPSS  A+FVVPAM NIQ AL+NAGL  +IKVSTA+ T ++  S+PPS G+ +N+ M L+
Sbjct: 121 QPSSSAASFVVPAMVNIQTALNNAGL-GKIKVSTAVATSIMADSYPPSRGTIKNEVMPLM 180

Query: 181 NPVIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAML 240
           NP+IRFL +NRSPLL+NLYPYFSYIGNP  IRLDYALFTAPS VV DG + Y+NLFDAML
Sbjct: 181 NPIIRFLNNNRSPLLLNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQYLYQNLFDAML 240

Query: 241 DAVYAALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRPI 300
           DA+YAALEKVGGGNLEIVISESGWPSAGGTATSI+NARTY NNLI+HVK GTPRRPGRP 
Sbjct: 241 DALYAALEKVGGGNLEIVISESGWPSAGGTATSINNARTYINNLIQHVKRGTPRRPGRPT 300

Query: 301 ETYIFAMFNENQKNPEFEKHFGLFSPNKQLKYPVNFN 338
           ETYIFAMF+EN+K+PE EKHFGLF PNKQ KYP+NFN
Sbjct: 301 ETYIFAMFDENKKSPELEKHFGLFFPNKQSKYPINFN 336

BLAST of Tan0012961 vs. TAIR 10
Match: AT3G57240.1 (beta-1,3-glucanase 3 )

HSP 1 Score: 411.4 bits (1056), Expect = 7.1e-115
Identity = 216/333 (64.86%), Postives = 257/333 (77.18%), Query Frame = 0

Query: 8   LSLCLLGLLLPNLHLTS-AQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYDPNRASL 67
           L L LL  +L +   T+  QIGVCYG+ GNNL P  EV+ L+ Q+NI+RMRLYDPN+ +L
Sbjct: 15  LLLLLLSFILASFFDTAVGQIGVCYGRNGNNLRPASEVVALYQQRNIRRMRLYDPNQETL 74

Query: 68  DALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFPNVRFKYIAVGNEVQPSSPT 127
           +ALRGSNIEL+L +PN DLQR+ SSQAEA+ W++NNVRN+ NV F+YI+VGNEVQPS   
Sbjct: 75  NALRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYISVGNEVQPSDQA 134

Query: 128 AAFVVPAMRNIQNALSNAGLAARIKVSTAIDT-GLIGVSFPPSSGSFRNDAMSLLNPVIR 187
           A+FV+PAM+NI+ A+S+ G    IKVSTAIDT G+ G  FPPSSG+F  +  S + PVI 
Sbjct: 135 ASFVLPAMQNIERAVSSLG----IKVSTAIDTRGISG--FPPSSGTFTPEFRSFIAPVIS 194

Query: 188 FLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAMLDAVYA 247
           FL   +SPLLVN YPYFSY GN   IRLDY LFTAPS VV DG   YRNLF A+LD VYA
Sbjct: 195 FLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVYA 254

Query: 248 ALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRPIETYIF 307
           +LEK GGG+LEIV+SESGWP+AGG AT +DNARTY NNLI+ VK G+PRRPGR  ETYIF
Sbjct: 255 SLEKAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKNGSPRRPGRATETYIF 314

Query: 308 AMFNENQK-NPEFEKHFGLFSPNKQLKYPVNFN 338
           AMF+EN K  PE EK +GLF PN Q KY VNFN
Sbjct: 315 AMFDENSKQGPETEKFWGLFLPNLQPKYVVNFN 341

BLAST of Tan0012961 vs. TAIR 10
Match: AT3G57270.1 (beta-1,3-glucanase 1 )

HSP 1 Score: 409.8 bits (1052), Expect = 2.1e-114
Identity = 207/336 (61.61%), Postives = 257/336 (76.49%), Query Frame = 0

Query: 4   FYSILSLCLLGLLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYDPNR 63
           F + L+L LLGL   N + T  Q+GVCYG+ GNNLP   E I LF QKNI+R+RLY P+ 
Sbjct: 5   FLASLTL-LLGLFFVNTNPTGGQVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDH 64

Query: 64  ASLDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNFPN-VRFKYIAVGNEVQP 123
             L ALRGSNIE+ LGLPNS LQ + SSQ++ANAW+Q  V N+ N VRF+YI+VGNEV+ 
Sbjct: 65  DVLAALRGSNIEVTLGLPNSYLQSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKI 124

Query: 124 SSPTAAFVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSLLNP 183
           S   A F+VPAM NI  A+  AGL  RIKVST++D G++  S+PPS GSFR D M ++ P
Sbjct: 125 SDSYAQFLVPAMENIDRAVLAAGLGGRIKVSTSVDMGVLRESYPPSKGSFRGDVMVVMEP 184

Query: 184 VIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAMLDA 243
           +IRFL+   SPLL+NLY YFSY GN   IRLDYALFTAPS +V+D P  Y+NLFDAMLDA
Sbjct: 185 IIRFLVSKNSPLLLNLYTYFSYAGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDA 244

Query: 244 VYAALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRPIET 303
           +Y+ALEK GG +LEIV++E+GWP+ GGT T+I+NAR YNNNLI+HVK GTP+RPG+ IET
Sbjct: 245 MYSALEKSGGASLEIVVAETGWPTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIET 304

Query: 304 YIFAMFNENQK--NPEFEKHFGLFSPNKQLKYPVNF 337
           Y+FA+++ENQK   P  EK +GLF PNKQ KY +NF
Sbjct: 305 YLFAIYDENQKPTPPYVEKFWGLFYPNKQPKYDINF 339

BLAST of Tan0012961 vs. TAIR 10
Match: AT4G16260.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 3.5e-106
Identity = 194/340 (57.06%), Postives = 250/340 (73.53%), Query Frame = 0

Query: 1   MANFYSILSLCLLGLLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYD 60
           M   + +++L +  +L P    +   +GVCYG MGNNLP Q + I LF Q NI+R+RLYD
Sbjct: 1   MTTLFLLIALFITTILNPT---SGESVGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYD 60

Query: 61  PNRASLDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRN-FPNVRFKYIAVGNE 120
           PN+A+L+ALR + IE+++G+PN+DL+ + ++ + A +W+QNNV N +P V FKYIAVGNE
Sbjct: 61  PNQAALNALRNTGIEVIIGVPNTDLRSL-TNPSSARSWLQNNVLNYYPAVSFKYIAVGNE 120

Query: 121 VQPSSPTAAFVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSL 180
           V PS+     V+PAMRN+ +AL  A L  RIKVSTAID  LIG SFPPSSG FR D    
Sbjct: 121 VSPSN-GGDVVLPAMRNVYDALRGANLQDRIKVSTAIDMTLIGNSFPPSSGEFRGDVRWY 180

Query: 181 LNPVIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAM 240
           ++PVI FL    S LL N+YPYFSY+ NP  I L YALFT+PSVVV DG  GY+NLFDA+
Sbjct: 181 IDPVIGFLTSTNSALLANIYPYFSYVDNPRDISLSYALFTSPSVVVWDGSRGYQNLFDAL 240

Query: 241 LDAVYAALEKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVK--GGTPRRPG 300
           LD VY+A+E+ GGG+L +V+SESGWPS GG A S DNAR +  NL   V+   GTP+RPG
Sbjct: 241 LDVVYSAVERSGGGSLPVVVSESGWPSNGGNAASFDNARAFYTNLASRVRENRGTPKRPG 300

Query: 301 RPIETYIFAMFNENQKNPEFEKHFGLFSPNKQLKYPVNFN 338
           R +ETY+FAMF+ENQK+PE EK+FGLF PNKQ K+P+ F+
Sbjct: 301 RGVETYLFAMFDENQKSPEIEKNFGLFFPNKQPKFPITFS 335

BLAST of Tan0012961 vs. TAIR 10
Match: AT3G57260.1 (beta-1,3-glucanase 2 )

HSP 1 Score: 375.2 bits (962), Expect = 5.6e-104
Identity = 191/330 (57.88%), Postives = 250/330 (75.76%), Query Frame = 0

Query: 10  LCLLGLLLPN-LHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYDPNRASLDA 69
           + LL L++ +  + T+ QIGVCYG +G+ LP   +V+ L+ Q+NI+RMRLY P+  +L A
Sbjct: 14  MILLSLVIASFFNHTAGQIGVCYGMLGDTLPSPSDVVALYKQQNIQRMRLYGPDPGALAA 73

Query: 70  LRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNVRNF-PNVRFKYIAVGNEVQPSSPTA 129
           LRGS+IEL+L +P+SDL+R+ SSQ EA+ W+Q NV+++   VRF+YI VGNEV+PS    
Sbjct: 74  LRGSDIELILDVPSSDLERLASSQTEADKWVQENVQSYRDGVRFRYINVGNEVKPS--VG 133

Query: 130 AFVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSLLNPVIRFL 189
            F++ AM+NI+NA+S AGL   +KVSTAI T     + PPS G FR++  S L PVI FL
Sbjct: 134 GFLLQAMQNIENAVSGAGL--EVKVSTAIATDTTTDTSPPSQGRFRDEYKSFLEPVIGFL 193

Query: 190 IDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGPFGYRNLFDAMLDAVYAAL 249
              +SPLLVNLYPYFSY+G+   I LDYALFTA S V  D  + Y+NLFDA LD+VYAAL
Sbjct: 194 ASKQSPLLVNLYPYFSYMGDTANIHLDYALFTAQSTVDNDPGYSYQNLFDANLDSVYAAL 253

Query: 250 EKVGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIRHVKGGTPRRPGRPIETYIFAM 309
           EK GGG+LEIV+SE+GWP+ G   TS++NA+TY NNLI+HVK G+PRRPG+ IETYIFAM
Sbjct: 254 EKSGGGSLEIVVSETGWPTEGAVGTSVENAKTYVNNLIQHVKNGSPRRPGKAIETYIFAM 313

Query: 310 FNENQKNPEFEKHFGLFSPNKQLKYPVNFN 338
           F+EN+K P +EK +GLF P++Q KY VNFN
Sbjct: 314 FDENKKEPTYEKFWGLFHPDRQSKYEVNFN 339

BLAST of Tan0012961 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 269.6 bits (688), Expect = 3.3e-72
Identity = 143/345 (41.45%), Postives = 218/345 (63.19%), Query Frame = 0

Query: 3   NFYSILSLCLLGLLLPNLHLTSAQIGVCYGQMGNNLPPQREVITLFNQKNIKRMRLYDPN 62
           +F  I S+ +L LLL   +    ++GVCYG+  ++LP   +V+ L  Q NIK +R+YD N
Sbjct: 4   DFKLIFSISILLLLLDCCY--GGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYN 63

Query: 63  RASLDALRGSNIELMLGLPNSDLQRIGSSQAEANAWIQNNV-RNFPNVRFKYIAVGNEV- 122
              L A   ++IELM+G+PNSDL     SQ+  + W++N+V   +P  +  YI VG E  
Sbjct: 64  SQVLKAFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAEST 123

Query: 123 -QPSSPTAAFVVPAMRNIQNALSNAGLAARIKVSTAIDTGLIGVSFPPSSGSFRNDAMSL 182
             P    ++FVVPAM+N+  AL   GL+ RIKVST +  G++  SFPPS+G+F +     
Sbjct: 124 DDPHINASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYF 183

Query: 183 LNPVIRFLIDNRSPLLVNLYPYFSYIGNPNAIRLDYALFTAPSVVVTDGP-FGYRNLFDA 242
           L P++ FL +N+SP +++LYPY++Y  +PN + LDY LF + S V+       Y+N+FDA
Sbjct: 184 LRPMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDA 243

Query: 243 MLDAVYAALEKVGGGNLEIVISESGWPSAGG----TATSIDNARTYNNNLIRHV--KGGT 302
            +DA+Y AL  +    ++I+++E+GWP+ G      A S DNA TYN+N+IRHV    GT
Sbjct: 244 QVDALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGT 303

Query: 303 PRRPGRPIETYIFAMFNENQK-NPEFEKHFGLFSPNKQLKYPVNF 337
           P +PG  +  YIF++FNEN+K   + E+++GLF P++   Y ++F
Sbjct: 304 PAKPGEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDF 346