Tan0012824 (gene) Snake gourd v1

Overview
NameTan0012824
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionCalcium-binding protein, putative
LocationLG02: 4137839 .. 4140678 (+)
RNA-Seq ExpressionTan0012824
SyntenyTan0012824
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAGATCAACGAACGACATTATCATTCACCGGAATCCAAATAAAGATCATGAAAGGACTTTTCAAGACGAAGCCACGATCGCCGGTGGAGCTCGTCCGGTACGCGCATGAGCTCCTTGTCTTCATTGACCGGAACGATGAGGTTCGTGAGCAAAAACGGGAAGAGAAGGTATTAATATTCTTATTGGGGTTGTTTTTCTGGATGATTTTGTTAAAAATTCATCTGGGTCACCGTTTTTTTATTGTATGGTTATCGATTTTCTCGATTTGCCTCTCGTTTTCTTCACCATTGTTCCCATTTAAGGTTGAAAATAGGGTATTGTTCTTTGAATTTCGTTTGTTAATTGTTGCAGTAGTTTTAAATTTGCCTTAGTTGCTTATATAAATCTTGAACTCGTGTAATGCATGTAATAGAAAATGCTGAAAAATGGGCTTGGACTTTTCTTTACAAGTGAAATTTGTTATTACATATTAGAAAATTTAGTTTATATGTGGCAGGAAATTGCATCTTTGATTGTGTTTTGTTTCCCCTTGATATTGAAAATGACTTTTGTGTTTGGATTGGAATTTGTGCAGATATCTGAACTGAATAAAATCATCTCTCAGATGAGAACGGTTCTTTATGGAAATGGTGAAACAGAGCCAAGTCCAGATGCTTGTGCTCAGTTGACTCAGGAGTTCTTCAAGGAGAATACGTTTCGCCTTTTTATCGCTTGCATTCCGAGGCTGAACCCAGGGGTGAATGAGCTTGCACTTGCAATTATATGATCATATTTTCATTTAAAACGTTGTTTTGGATAAGTCCCTGCATTCATTTCATAAACCTGCAAGTTCTTGAAGTTGTGTAGCTTGACTAGTGTGTATCCTTATCTGCAGTTGCGACAATGTGCAACTCATGTTCTTGCCAATCTGCAGCGGCAGCAGGTTAAGTCACGGATAATCGCCTCCGAGTACTTGGAGAACAACATGGATGTTATGGACATTCTGATACCAGGGTAAGAAGTGGGCATTGTTTTAAGATTGAACTATCAAATTGTTGACATTTCAAGACATCCATTGATGGTTAATGATTGGCTCAATTAGGTATGAAGACAGCGACATTGCTTTAACTTATGGCGCAATTTTGAGAGAATGCATCCGTCACCAGTGCGTTGCTAGGTATGAACCAGTTAATTCTTTTATTGCAAACATCTTTCTCAAAGAGAAGTATCATTTTGACATTTCTGTGCAGATGTTTGAAACCTCGAATAACTGGCAAAACTATTTAGTGCTGTATGTACCACGGCATTATGAATAGCTCTAGTTAAATGTTCATATTTTCTTCTTCTTTTTCTTTTTCTCTCTCGTAATCCCTAATCGTTTCCGGTTCAGGTGTGTCCTGGAATCAGAGCATGTGAGGAAGTTTTTTGATTACGTACAAAATCCAATATTTTACGTTGCATCAGATGCTTTTGTAACTTTTAAAGTTAAGACTGAATTTCTTCTCTCTTCTTTATTTATCATTTCTTATGTTCATTATGTCTTCTGTAGTTCTGTATCTTTATGTACCAATTGCTCACAATGGTGACAACCAAATTAACATGCCAACTTCAGCATAGTCCAACTACCTAAGGCATATACCCTCGACGAAGATGTTAGATATTCAAATCTTCCCACCCCACATGTCGAACTAAAAAAAAAAAAATCAAATTAACCTTCTTTTATGCCACACCGAGTAAAAATTAGCTGTAGTATGTTTTGGCATATAAAGAGAATGCTGTATGTCAACTAAAACATTCTGTACTAATTTTATGCAGAAACTTTTGACAAGGCATAAGTCTACTGTTGCTGCATTTCTTACTAAAAATTATGACTGGGTAAGCATTAGTTAAGTTACTTTCATTAGAATGTATTTGGTGCCATTTGCGTTGAGATGAACAAGTTCATTGAAGTTGTTTTGCAATCCTTTCAGTTCTTTGAAGAGTACAATACACAATTGCTGGAATCGACTAACTACATCACGAAACGACACGCTGTGAAGGTAAAGAGAGAACGAATGCCTATGACAAGCATGCTAATAGCCCTGTTTATAATTTACATGTTTAGATTCACTCTATCACAACCAATGGACACATTATGATTCAAAGATTATGTATTTCCATGATGAGAAATCAATAAGCATTTGCAGCCATTTTATGATATTAATGTTGGGTACTCTGCTTCAGTTGTTGGGCGATATACTACTCGATAACTCTAATGCCGCTGTGATGGTTCGATATGTGAGCTCATTGGATAACATGAGGATCTTGATGAATCTTCTAAGGGTAAACTCTCTCACTTTTAAACATGTGCATACAACTTTTTAAGGATTTACACCCTTCCTTTTTTGTCTCGCCCCACATATTATACTTATAGGATCCAAACAAGATGCTACAAAGAGAGGCCTTCGATGTATTCAAGGTACTATCAAAGATTGTTGAATATTGATTTTGCACTACTAAGTTTTTTTTTTTGGTTAAAGAAGTTTTTTGTTTTGTTTAATAGCTATTTGTTGCAAATAAAAACAAGCCTCTAGAGATCACAGGTATACTTGTTGCTAATAGAACGAAACTTCTTCGATTTCTCGACGACCTTAAATTAGATAAAGGTAATAACTAAAGAAGTTAAACTTCTGCTCCTGTTCTCTGACTACTTAATATTAATTCATCCATCGTATGTACATGAATGATTGATCACTTTTCAGTTCCAGCGAATGAAGGATTTGAAGAAGATAAAACTCAGGTTATAAGAGAAATCTCCATCCTCGTGTTAGGCGATCTCTCGTCTTCGGAGATGGAAAACTGCAAGATCGAATATTGA

mRNA sequence

AAAAAAGATCAACGAACGACATTATCATTCACCGGAATCCAAATAAAGATCATGAAAGGACTTTTCAAGACGAAGCCACGATCGCCGGTGGAGCTCGTCCGGTACGCGCATGAGCTCCTTGTCTTCATTGACCGGAACGATGAGGTTCGTGAGCAAAAACGGGAAGAGAAGATATCTGAACTGAATAAAATCATCTCTCAGATGAGAACGGTTCTTTATGGAAATGGTGAAACAGAGCCAAGTCCAGATGCTTGTGCTCAGTTGACTCAGGAGTTCTTCAAGGAGAATACGTTTCGCCTTTTTATCGCTTGCATTCCGAGGCTGAACCCAGGGCGGCAGCAGGTTAAGTCACGGATAATCGCCTCCGAGTACTTGGAGAACAACATGGATGTTATGGACATTCTGATACCAGGGTATGAAGACAGCGACATTGCTTTAACTTATGGCGCAATTTTGAGAGAATGCATCCGTCACCAGTGCGTTGCTAGGTGTGTCCTGGAATCAGAGCATGTGAGGAAGTTTTTTGATTACGTACAAAATCCAATATTTTACGTTGCATCAGATGCTTTTGTAACTTTTAAAAAACTTTTGACAAGGCATAAGTCTACTGTTGCTGCATTTCTTACTAAAAATTATGACTGGTTCTTTGAAGAGTACAATACACAATTGCTGGAATCGACTAACTACATCACGAAACGACACGCTGTGAAGTTGTTGGGCGATATACTACTCGATAACTCTAATGCCGCTGTGATGGTTCGATATGTGAGCTCATTGGATAACATGAGGATCTTGATGAATCTTCTAAGGGATCCAAACAAGATGCTACAAAGAGAGGCCTTCGATGTATTCAAGCTATTTGTTGCAAATAAAAACAAGCCTCTAGAGATCACAGGTATACTTGTTGCTAATAGAACGAAACTTCTTCGATTTCTCGACGACCTTAAATTAGATAAAGCGAATGAAGGATTTGAAGAAGATAAAACTCAGGTTATAAGAGAAATCTCCATCCTCGTGTTAGGCGATCTCTCGTCTTCGGAGATGGAAAACTGCAAGATCGAATATTGA

Coding sequence (CDS)

ATGAAAGGACTTTTCAAGACGAAGCCACGATCGCCGGTGGAGCTCGTCCGGTACGCGCATGAGCTCCTTGTCTTCATTGACCGGAACGATGAGGTTCGTGAGCAAAAACGGGAAGAGAAGATATCTGAACTGAATAAAATCATCTCTCAGATGAGAACGGTTCTTTATGGAAATGGTGAAACAGAGCCAAGTCCAGATGCTTGTGCTCAGTTGACTCAGGAGTTCTTCAAGGAGAATACGTTTCGCCTTTTTATCGCTTGCATTCCGAGGCTGAACCCAGGGCGGCAGCAGGTTAAGTCACGGATAATCGCCTCCGAGTACTTGGAGAACAACATGGATGTTATGGACATTCTGATACCAGGGTATGAAGACAGCGACATTGCTTTAACTTATGGCGCAATTTTGAGAGAATGCATCCGTCACCAGTGCGTTGCTAGGTGTGTCCTGGAATCAGAGCATGTGAGGAAGTTTTTTGATTACGTACAAAATCCAATATTTTACGTTGCATCAGATGCTTTTGTAACTTTTAAAAAACTTTTGACAAGGCATAAGTCTACTGTTGCTGCATTTCTTACTAAAAATTATGACTGGTTCTTTGAAGAGTACAATACACAATTGCTGGAATCGACTAACTACATCACGAAACGACACGCTGTGAAGTTGTTGGGCGATATACTACTCGATAACTCTAATGCCGCTGTGATGGTTCGATATGTGAGCTCATTGGATAACATGAGGATCTTGATGAATCTTCTAAGGGATCCAAACAAGATGCTACAAAGAGAGGCCTTCGATGTATTCAAGCTATTTGTTGCAAATAAAAACAAGCCTCTAGAGATCACAGGTATACTTGTTGCTAATAGAACGAAACTTCTTCGATTTCTCGACGACCTTAAATTAGATAAAGCGAATGAAGGATTTGAAGAAGATAAAACTCAGGTTATAAGAGAAATCTCCATCCTCGTGTTAGGCGATCTCTCGTCTTCGGAGATGGAAAACTGCAAGATCGAATATTGA

Protein sequence

MKGLFKTKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGETEPSPDACAQLTQEFFKENTFRLFIACIPRLNPGRQQVKSRIIASEYLENNMDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFYVASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILLDNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVANRTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLSSSEMENCKIEY
Homology
[Close]
BLAST of Tan0012824 vs. ExPASy Swiss-Prot
Match: Q9FGK3 (Putative MO25-like protein At5g47540 OS=Arabidopsis thaliana OX=3702 GN=At5g47540 PE=2 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 8.2e-116
Identity = 213/343 (62.10%), Postives = 271/343 (79.01%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRN---DEVREQKREEKISELNKIISQMRTVLYG 60
           MKGLFK+KPR+P +LVR   +LL+F DR+    ++R+ KR+EK++EL++ I  M+++LYG
Sbjct: 1   MKGLFKSKPRTPADLVRQTRDLLLFSDRSTSLPDLRDSKRDEKMAELSRNIRDMKSILYG 60

Query: 61  NGETEPSPDACAQLTQEFFKENTFRLFIACIPRL-------------NPGRQQVKSRIIA 120
           N E EP  +ACAQLTQEFFKE+T RL I C+P+L             N  RQQV SR+IA
Sbjct: 61  NSEAEPVAEACAQLTQEFFKEDTLRLLITCLPKLNLETRKDATQVVANLQRQQVNSRLIA 120

Query: 121 SEYLENNMDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNP 180
           S+YLE N+D+MD+LI G+E++D+AL YGA+ RECIRHQ VA+ VLES+HV+KFFDY+Q P
Sbjct: 121 SDYLEANIDLMDVLIEGFENTDMALHYGAMFRECIRHQIVAKYVLESDHVKKFFDYIQLP 180

Query: 181 IFYVASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGD 240
            F +A+DA  TFK+LLTRHKSTVA FLTKN DWFF +YN++LLES+NYIT+R A+KLLGD
Sbjct: 181 NFDIAADAAATFKELLTRHKSTVAEFLTKNEDWFFADYNSKLLESSNYITRRQAIKLLGD 240

Query: 241 ILLDNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGIL 300
           ILLD SN+AVM +YVSS DN+RILMNLLR+ +K +Q EAF VFKLF AN+NKP +I  IL
Sbjct: 241 ILLDRSNSAVMTKYVSSRDNLRILMNLLRESSKSIQIEAFHVFKLFAANQNKPADIVNIL 300

Query: 301 VANRTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLS 328
           VANR+KLLR L DLK DK +E FE DK+QV+REI+ L   DL+
Sbjct: 301 VANRSKLLRLLADLKPDKEDERFEADKSQVLREIAALEPRDLA 343

[Close]
BLAST of Tan0012824 vs. ExPASy Swiss-Prot
Match: Q9M0M4 (Putative MO25-like protein At4g17270 OS=Arabidopsis thaliana OX=3702 GN=At4g17270 PE=2 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 1.8e-110
Identity = 203/343 (59.18%), Postives = 264/343 (76.97%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRND---EVREQKREEKISELNKIISQMRTVLYG 60
           M+GLFK+KPR+P ++VR   +LL++ DR++   ++RE KREEK+ EL+K I  ++ +LYG
Sbjct: 1   MRGLFKSKPRTPADIVRQTRDLLLYADRSNSFPDLRESKREEKMVELSKSIRDLKLILYG 60

Query: 61  NGETEPSPDACAQLTQEFFKENTFRLFIACIPRL-------------NPGRQQVKSRIIA 120
           N E EP  +ACAQLTQEFFK +T R  +  +P L             N  RQQV SR+IA
Sbjct: 61  NSEAEPVAEACAQLTQEFFKADTLRRLLTSLPNLNLEARKDATQVVANLQRQQVNSRLIA 120

Query: 121 SEYLENNMDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNP 180
           ++YLE+N+D+MD L+ G+E++D+AL YG + RECIRHQ VA+ VL+SEHV+KFF Y+Q P
Sbjct: 121 ADYLESNIDLMDFLVDGFENTDMALHYGTMFRECIRHQIVAKYVLDSEHVKKFFYYIQLP 180

Query: 181 IFYVASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGD 240
            F +A+DA  TFK+LLTRHKSTVA FL KN DWFF +YN++LLESTNYIT+R A+KLLGD
Sbjct: 181 NFDIAADAAATFKELLTRHKSTVAEFLIKNEDWFFADYNSKLLESTNYITRRQAIKLLGD 240

Query: 241 ILLDNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGIL 300
           ILLD SN+AVM +YVSS+DN+RILMNLLR+ +K +Q EAF VFKLFVAN+NKP +I  IL
Sbjct: 241 ILLDRSNSAVMTKYVSSMDNLRILMNLLRESSKTIQIEAFHVFKLFVANQNKPSDIANIL 300

Query: 301 VANRTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLS 328
           VANR KLLR L D+K DK +E F+ DK QV+REI+ L L +L+
Sbjct: 301 VANRNKLLRLLADIKPDKEDERFDADKAQVVREIANLKLRELA 343

[Close]
BLAST of Tan0012824 vs. ExPASy Swiss-Prot
Match: Q9ZQ77 (MO25-like protein At2g03410 OS=Arabidopsis thaliana OX=3702 GN=At2g03410 PE=2 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 3.8e-97
Identity = 186/347 (53.60%), Postives = 247/347 (71.18%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRNDE---VREQKREEKISELNKIISQMRTVLYG 60
           MKGLFK K R P E+VR   +L+   +  +E    R  KR    +EL + I  ++++LYG
Sbjct: 1   MKGLFKNKSRLPGEIVRQTRDLIALAESEEEETDARNSKRLGICAELCRNIRDLKSILYG 60

Query: 61  NGETEPSPDACAQLTQEFFKENTFRLFIACIPRL-------------NPGRQQVKSRIIA 120
           NGE EP P+AC  LTQEFF+ +T R  I  IP+L             N  +QQV+ R++A
Sbjct: 61  NGEAEPVPEACLLLTQEFFRADTLRPLIKSIPKLDLEARKDATQIVANLQKQQVEFRLVA 120

Query: 121 SEYLENNMDVMDILIPGYE-DSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQN 180
           SEYLE+N+DV+D L+ G + D ++AL Y  +L+EC+RHQ VA+ +LES+++ KFFDYVQ 
Sbjct: 121 SEYLESNLDVIDSLVEGIDHDHELALHYTGMLKECVRHQVVAKYILESKNLEKFFDYVQL 180

Query: 181 PIFYVASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLG 240
           P F VA+DA   F++LLTRHKSTVA +L KNY+WFF EYNT+LLE  +Y TKR A KLLG
Sbjct: 181 PYFDVATDASKIFRELLTRHKSTVAEYLAKNYEWFFAEYNTKLLEKGSYFTKRQASKLLG 240

Query: 241 DILLDNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGI 300
           D+L+D SN+ VMV+YVSSLDN+RI+MNLLR+P K +Q EAF +FKLFVAN+NKP +I  I
Sbjct: 241 DVLMDRSNSGVMVKYVSSLDNLRIMMNLLREPTKNIQLEAFHIFKLFVANENKPEDIVAI 300

Query: 301 LVANRTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLSSSE 331
           LVANRTK+LR   DLK +K + GFE DK  V+ EI+ L L D+ +++
Sbjct: 301 LVANRTKILRLFADLKPEKEDVGFETDKALVMNEIATLSLLDIKTAD 347

[Close]
BLAST of Tan0012824 vs. ExPASy Swiss-Prot
Match: Q06138 (Calcium-binding protein 39 OS=Mus musculus OX=10090 GN=Cab39 PE=1 SV=2)

HSP 1 Score: 243.0 bits (619), Expect = 4.7e-63
Identity = 131/328 (39.94%), Postives = 202/328 (61.59%), Query Frame = 0 

Query: 5   FKTKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGETEPS 64
           F    +SP ++V+   E +  +++ D + ++K E+   E++K +  M+ +LYG  E EP 
Sbjct: 5   FGKSHKSPADIVKNLKESMAVLEKQD-ISDKKAEKATEEVSKNLVAMKEILYGTNEKEPQ 64

Query: 65  PDACAQLTQEFFKENTFRLFIACIPRL-------------NPGRQQVKSRIIASEYLENN 124
            +A AQL QE +        +A +  +             N  R+Q+ +R    EY+   
Sbjct: 65  TEAVAQLAQELYNSGLLGTLVADLQLIDFEGKKDVAQIFNNILRRQIGTRTPTVEYICTQ 124

Query: 125 MDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFYVASD 184
            +++ +L+ GYE  +IAL  G +LRECIRH+ +A+ +L SE    FF YV+   F +ASD
Sbjct: 125 QNILFMLLKGYESPEIALNCGIMLRECIRHEPLAKIILWSEQFYDFFRYVEMSTFDIASD 184

Query: 185 AFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILLDNSN 244
           AF TFK LLTRHK   A FL ++YD FF EY  +LL S NY+TKR ++KLLG++LLD  N
Sbjct: 185 AFATFKDLLTRHKLLSAEFLEQHYDRFFSEYE-KLLHSENYVTKRQSLKLLGELLLDRHN 244

Query: 245 AAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVANRTKL 304
             +M +Y+S  +N++++MNLLRD ++ +Q EAF VFK+FVAN NK   I  IL+ N+TKL
Sbjct: 245 FTIMTKYISKPENLKLMMNLLRDKSRNIQFEAFHVFKVFVANPNKTQPILDILLKNQTKL 304

Query: 305 LRFLDDLKLDKA-NEGFEEDKTQVIREI 319
           + FL   + D+  +E F ++KT ++++I
Sbjct: 305 IEFLSKFQNDRTEDEQFNDEKTYLVKQI 330

[Close]
BLAST of Tan0012824 vs. ExPASy Swiss-Prot
Match: Q29RI6 (Calcium-binding protein 39 OS=Bos taurus OX=9913 GN=CAB39 PE=2 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.4e-62
Identity = 130/328 (39.63%), Postives = 201/328 (61.28%), Query Frame = 0 

Query: 5   FKTKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGETEPS 64
           F    +SP ++V+   E +  +++ D + ++K E+   E++K +  M+ +LYG  E EP 
Sbjct: 5   FGKSHKSPADIVKNLKESMAVLEKQD-ISDKKAEKATEEVSKNLVAMKEILYGTNEKEPQ 64

Query: 65  PDACAQLTQEFFKENTFRLFIACIPRL-------------NPGRQQVKSRIIASEYLENN 124
            +A AQL QE +        +A +  +             N  R+Q+ +R    EY+   
Sbjct: 65  TEAVAQLAQELYNSGLLSTLVADLQLIDFEGKKDVAQIFNNILRRQIGTRTPTVEYICTQ 124

Query: 125 MDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFYVASD 184
            +++ +L+ GYE  +IAL  G +LRECIRH+ +A+ +L SE    FF YV+   F +ASD
Sbjct: 125 QNILFMLLKGYESPEIALNCGIMLRECIRHEPLAKIILWSEQFYDFFRYVEMSTFDIASD 184

Query: 185 AFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILLDNSN 244
           AF TFK LLTRHK   A FL ++YD FF EY  +LL S NY+TKR ++KLLG++LLD  N
Sbjct: 185 AFATFKDLLTRHKLLSAEFLEQHYDRFFSEYE-KLLHSENYVTKRQSLKLLGELLLDRHN 244

Query: 245 AAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVANRTKL 304
             +M +Y+S  +N++++MNLLRD ++ +Q EAF VFK+FVAN NK   I  IL+ N+ KL
Sbjct: 245 FTIMTKYISKPENLKLMMNLLRDKSRNIQFEAFHVFKVFVANPNKTQPILDILLKNQAKL 304

Query: 305 LRFLDDLKLDKA-NEGFEEDKTQVIREI 319
           + FL   + D+  +E F ++KT ++++I
Sbjct: 305 IEFLSKFQNDRTEDEQFNDEKTYLVKQI 330

[Close]
BLAST of Tan0012824 vs. NCBI nr
Match: XP_038887041.1 (putative MO25-like protein At5g47540 isoform X2 [Benincasa hispida])

HSP 1 Score: 579.3 bits (1492), Expect = 2.1e-161
Identity = 292/333 (87.69%), Postives = 309/333 (92.79%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGE 60
           MKGLFK K RSPVELVRYAHELL+FIDRN+EVREQKR EK+SELNK ISQMRTVLYGN E
Sbjct: 50  MKGLFKPKTRSPVELVRYAHELLLFIDRNEEVREQKRAEKMSELNKTISQMRTVLYGNAE 109

Query: 61  TEPSPDACAQLTQEFFKENTFRLFIACIPRLNPGRQQVKSRIIASEYLENNMDVMDILIP 120
            EPSPDAC+QLTQEFFKEN FRLFIACIP+LN GRQQVKSRIIASEYLENNMD+MDILIP
Sbjct: 110 GEPSPDACSQLTQEFFKENMFRLFIACIPKLNSGRQQVKSRIIASEYLENNMDIMDILIP 169

Query: 121 GYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFYVASDAFVTFKKLL 180
           GYEDSDIALTYGAI RECIRHQCVAR VLES H++KFFDY+QNPIFYVASDA  TF+KLL
Sbjct: 170 GYEDSDIALTYGAIARECIRHQCVARYVLESVHMKKFFDYIQNPIFYVASDASATFRKLL 229

Query: 181 TRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILLDNSNAAVMVRYVS 240
           TRHKSTVAAFLTKNYDWFF+EYN QLLESTNYITKRHAVKLLGDILLDNSN+AVMV+YVS
Sbjct: 230 TRHKSTVAAFLTKNYDWFFKEYNMQLLESTNYITKRHAVKLLGDILLDNSNSAVMVQYVS 289

Query: 241 SLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVANRTKLLRFLDDLKL 300
           SLDNMRILMNLLRDPNK +QR+AFDVFKLFVANKNKP EIT ILVANRTKLLRFL+DLK 
Sbjct: 290 SLDNMRILMNLLRDPNKTIQRDAFDVFKLFVANKNKPPEITSILVANRTKLLRFLNDLKP 349

Query: 301 DKANEGFEEDKTQVIREISILVLGDLSSSEMEN 334
           DK NEGFEEDK Q+IREISIL   D SSSEMEN
Sbjct: 350 DKVNEGFEEDKAQIIREISILESSDPSSSEMEN 382

[Close]
BLAST of Tan0012824 vs. NCBI nr
Match: XP_038887040.1 (putative MO25-like protein At5g47540 isoform X1 [Benincasa hispida])

HSP 1 Score: 570.1 bits (1468), Expect = 1.3e-158
Identity = 292/346 (84.39%), Postives = 309/346 (89.31%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGE 60
           MKGLFK K RSPVELVRYAHELL+FIDRN+EVREQKR EK+SELNK ISQMRTVLYGN E
Sbjct: 50  MKGLFKPKTRSPVELVRYAHELLLFIDRNEEVREQKRAEKMSELNKTISQMRTVLYGNAE 109

Query: 61  TEPSPDACAQLTQEFFKENTFRLFIACIPRLNPG-------------RQQVKSRIIASEY 120
            EPSPDAC+QLTQEFFKEN FRLFIACIP+LN G             RQQVKSRIIASEY
Sbjct: 110 GEPSPDACSQLTQEFFKENMFRLFIACIPKLNSGLRQCATHVLANLQRQQVKSRIIASEY 169

Query: 121 LENNMDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFY 180
           LENNMD+MDILIPGYEDSDIALTYGAI RECIRHQCVAR VLES H++KFFDY+QNPIFY
Sbjct: 170 LENNMDIMDILIPGYEDSDIALTYGAIARECIRHQCVARYVLESVHMKKFFDYIQNPIFY 229

Query: 181 VASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILL 240
           VASDA  TF+KLLTRHKSTVAAFLTKNYDWFF+EYN QLLESTNYITKRHAVKLLGDILL
Sbjct: 230 VASDASATFRKLLTRHKSTVAAFLTKNYDWFFKEYNMQLLESTNYITKRHAVKLLGDILL 289

Query: 241 DNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVAN 300
           DNSN+AVMV+YVSSLDNMRILMNLLRDPNK +QR+AFDVFKLFVANKNKP EIT ILVAN
Sbjct: 290 DNSNSAVMVQYVSSLDNMRILMNLLRDPNKTIQRDAFDVFKLFVANKNKPPEITSILVAN 349

Query: 301 RTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLSSSEMEN 334
           RTKLLRFL+DLK DK NEGFEEDK Q+IREISIL   D SSSEMEN
Sbjct: 350 RTKLLRFLNDLKPDKVNEGFEEDKAQIIREISILESSDPSSSEMEN 395

[Close]
BLAST of Tan0012824 vs. NCBI nr
Match: KAG6589115.1 (putative MO25-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 567.0 bits (1460), Expect = 1.1e-157
Identity = 284/350 (81.14%), Postives = 313/350 (89.43%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGE 60
           MKGLFK KPRSP+ELVRYAHELL+FIDR+D VREQKREEKISELNK ISQMRT+LYGNGE
Sbjct: 1   MKGLFKPKPRSPLELVRYAHELLLFIDRSDGVREQKREEKISELNKTISQMRTILYGNGE 60

Query: 61  TEPSPDACAQLTQEFFKENTFRLFIACIPRLNPG-------------RQQVKSRIIASEY 120
            EP+PDACAQLTQEFF+ENTFRLFIACIPRLN G             RQQVKSR+IAS+Y
Sbjct: 61  EEPNPDACAQLTQEFFRENTFRLFIACIPRLNSGLRQCATHVLANLQRQQVKSRMIASDY 120

Query: 121 LENNMDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFY 180
           LENNMD+MDILIPGYEDSDIALTYGAI  ECIRHQCVA+ +LESEH++KFFDYVQNPIFY
Sbjct: 121 LENNMDIMDILIPGYEDSDIALTYGAIASECIRHQCVAKYILESEHIKKFFDYVQNPIFY 180

Query: 181 VASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILL 240
           VASDA  TF+KLLTRHKSTV  FLT NY+WFFEEYNTQLLES NYITKRHAVKLLGD+LL
Sbjct: 181 VASDASATFRKLLTRHKSTVTGFLTTNYEWFFEEYNTQLLESPNYITKRHAVKLLGDLLL 240

Query: 241 DNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVAN 300
           DNSNA VMVRYVSSLDNMRILMNLLRDPNKM+QR+AF VFKLFVANKNKPLEITG+LVAN
Sbjct: 241 DNSNAVVMVRYVSSLDNMRILMNLLRDPNKMIQRDAFHVFKLFVANKNKPLEITGVLVAN 300

Query: 301 RTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLSSSEMENCKIE 338
           RTKLLR LD+L+LDK +EGFEEDK QV+REIS+L  GDL +SE++  +I+
Sbjct: 301 RTKLLRLLDELRLDKDDEGFEEDKAQVMREISMLETGDLWASEIQKDEIQ 350

[Close]
BLAST of Tan0012824 vs. NCBI nr
Match: XP_022989431.1 (putative MO25-like protein At5g47540 [Cucurbita maxima])

HSP 1 Score: 566.2 bits (1458), Expect = 1.9e-157
Identity = 285/350 (81.43%), Postives = 313/350 (89.43%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGE 60
           MKGLFK KPRSP+ELVRYAHELL+FIDR+D VREQKREEKISELNK ISQMRT+LYGNGE
Sbjct: 1   MKGLFKPKPRSPLELVRYAHELLLFIDRSDGVREQKREEKISELNKTISQMRTILYGNGE 60

Query: 61  TEPSPDACAQLTQEFFKENTFRLFIACIPRLNPG-------------RQQVKSRIIASEY 120
            EP+PDACAQLTQEFF+ENTFRLFIACIPRLN G             RQQVKSR+IAS+Y
Sbjct: 61  EEPNPDACAQLTQEFFRENTFRLFIACIPRLNSGLRQCATHVLANLQRQQVKSRMIASDY 120

Query: 121 LENNMDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFY 180
           LENNMD+MDILIPGYEDSDIALTYGAI  ECIRHQCVA+ +LESEH++KFF YVQNPIFY
Sbjct: 121 LENNMDIMDILIPGYEDSDIALTYGAIASECIRHQCVAKYILESEHIKKFFYYVQNPIFY 180

Query: 181 VASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILL 240
           VASDA  TF+KLLTRHKSTV  FLT NY+WFFEEYNTQLLES+NYITKRHAVKLLGD+LL
Sbjct: 181 VASDASATFRKLLTRHKSTVTGFLTTNYEWFFEEYNTQLLESSNYITKRHAVKLLGDLLL 240

Query: 241 DNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVAN 300
           DNSNA VMVRYVSSLDNMRILMNLLRDPNKM+QR+AF VFKLFVANKNKPLEITG+LVAN
Sbjct: 241 DNSNAVVMVRYVSSLDNMRILMNLLRDPNKMIQRDAFHVFKLFVANKNKPLEITGVLVAN 300

Query: 301 RTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLSSSEMENCKIE 338
           RTKLLR LD+L+LDK +EGFEEDK QV+REIS+L  GDL SSE+E  +I+
Sbjct: 301 RTKLLRLLDELRLDKDDEGFEEDKAQVMREISMLEKGDLWSSEIEKDEIQ 350

[Close]
BLAST of Tan0012824 vs. NCBI nr
Match: XP_022928128.1 (putative MO25-like protein At5g47540 [Cucurbita moschata])

HSP 1 Score: 566.2 bits (1458), Expect = 1.9e-157
Identity = 284/350 (81.14%), Postives = 312/350 (89.14%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGE 60
           MKGLFK KPRSP+ELVRYAHELL+FIDR+D VREQKREEKISELNK ISQMRT+LYGNGE
Sbjct: 1   MKGLFKPKPRSPLELVRYAHELLLFIDRSDGVREQKREEKISELNKTISQMRTILYGNGE 60

Query: 61  TEPSPDACAQLTQEFFKENTFRLFIACIPRLNPG-------------RQQVKSRIIASEY 120
            EP+PDACAQLTQEFF+ENTF LFIACIPRLN G             RQQVKSR+IAS+Y
Sbjct: 61  EEPNPDACAQLTQEFFRENTFHLFIACIPRLNSGLRQCATHVLANLQRQQVKSRMIASDY 120

Query: 121 LENNMDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFY 180
           LENNMD+MDILIPGYEDSDIALTYGAI  ECIRHQCVA+ +LESEH++KFFDYVQNPIFY
Sbjct: 121 LENNMDIMDILIPGYEDSDIALTYGAIASECIRHQCVAKYILESEHIKKFFDYVQNPIFY 180

Query: 181 VASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILL 240
           VASDA  TF+KLLTRHKSTV  FLT NY+WFFEEYNTQLLES NYITKRHAVKLLGD+LL
Sbjct: 181 VASDASATFRKLLTRHKSTVTGFLTTNYEWFFEEYNTQLLESPNYITKRHAVKLLGDLLL 240

Query: 241 DNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVAN 300
           DNSNA VMVRYVSSLDNMRILMNLLRDPNKM+QR+AF VFKLFVANKNKPLEITG+LVAN
Sbjct: 241 DNSNAVVMVRYVSSLDNMRILMNLLRDPNKMIQRDAFHVFKLFVANKNKPLEITGVLVAN 300

Query: 301 RTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLSSSEMENCKIE 338
           RTKLLR LD+L+LDK +EGFEEDK QV+REIS+L  GDL +SE+E  +I+
Sbjct: 301 RTKLLRLLDELRLDKDDEGFEEDKAQVMREISMLETGDLWASEIEKDEIQ 350

[Close]
BLAST of Tan0012824 vs. ExPASy TrEMBL
Match: A0A6J1JK19 (putative MO25-like protein At5g47540 OS=Cucurbita maxima OX=3661 GN=LOC111486487 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 9.0e-158
Identity = 285/350 (81.43%), Postives = 313/350 (89.43%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGE 60
           MKGLFK KPRSP+ELVRYAHELL+FIDR+D VREQKREEKISELNK ISQMRT+LYGNGE
Sbjct: 1   MKGLFKPKPRSPLELVRYAHELLLFIDRSDGVREQKREEKISELNKTISQMRTILYGNGE 60

Query: 61  TEPSPDACAQLTQEFFKENTFRLFIACIPRLNPG-------------RQQVKSRIIASEY 120
            EP+PDACAQLTQEFF+ENTFRLFIACIPRLN G             RQQVKSR+IAS+Y
Sbjct: 61  EEPNPDACAQLTQEFFRENTFRLFIACIPRLNSGLRQCATHVLANLQRQQVKSRMIASDY 120

Query: 121 LENNMDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFY 180
           LENNMD+MDILIPGYEDSDIALTYGAI  ECIRHQCVA+ +LESEH++KFF YVQNPIFY
Sbjct: 121 LENNMDIMDILIPGYEDSDIALTYGAIASECIRHQCVAKYILESEHIKKFFYYVQNPIFY 180

Query: 181 VASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILL 240
           VASDA  TF+KLLTRHKSTV  FLT NY+WFFEEYNTQLLES+NYITKRHAVKLLGD+LL
Sbjct: 181 VASDASATFRKLLTRHKSTVTGFLTTNYEWFFEEYNTQLLESSNYITKRHAVKLLGDLLL 240

Query: 241 DNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVAN 300
           DNSNA VMVRYVSSLDNMRILMNLLRDPNKM+QR+AF VFKLFVANKNKPLEITG+LVAN
Sbjct: 241 DNSNAVVMVRYVSSLDNMRILMNLLRDPNKMIQRDAFHVFKLFVANKNKPLEITGVLVAN 300

Query: 301 RTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLSSSEMENCKIE 338
           RTKLLR LD+L+LDK +EGFEEDK QV+REIS+L  GDL SSE+E  +I+
Sbjct: 301 RTKLLRLLDELRLDKDDEGFEEDKAQVMREISMLEKGDLWSSEIEKDEIQ 350

[Close]
BLAST of Tan0012824 vs. ExPASy TrEMBL
Match: A0A6J1EN08 (putative MO25-like protein At5g47540 OS=Cucurbita moschata OX=3662 GN=LOC111435045 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 9.0e-158
Identity = 284/350 (81.14%), Postives = 312/350 (89.14%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGE 60
           MKGLFK KPRSP+ELVRYAHELL+FIDR+D VREQKREEKISELNK ISQMRT+LYGNGE
Sbjct: 1   MKGLFKPKPRSPLELVRYAHELLLFIDRSDGVREQKREEKISELNKTISQMRTILYGNGE 60

Query: 61  TEPSPDACAQLTQEFFKENTFRLFIACIPRLNPG-------------RQQVKSRIIASEY 120
            EP+PDACAQLTQEFF+ENTF LFIACIPRLN G             RQQVKSR+IAS+Y
Sbjct: 61  EEPNPDACAQLTQEFFRENTFHLFIACIPRLNSGLRQCATHVLANLQRQQVKSRMIASDY 120

Query: 121 LENNMDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFY 180
           LENNMD+MDILIPGYEDSDIALTYGAI  ECIRHQCVA+ +LESEH++KFFDYVQNPIFY
Sbjct: 121 LENNMDIMDILIPGYEDSDIALTYGAIASECIRHQCVAKYILESEHIKKFFDYVQNPIFY 180

Query: 181 VASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILL 240
           VASDA  TF+KLLTRHKSTV  FLT NY+WFFEEYNTQLLES NYITKRHAVKLLGD+LL
Sbjct: 181 VASDASATFRKLLTRHKSTVTGFLTTNYEWFFEEYNTQLLESPNYITKRHAVKLLGDLLL 240

Query: 241 DNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVAN 300
           DNSNA VMVRYVSSLDNMRILMNLLRDPNKM+QR+AF VFKLFVANKNKPLEITG+LVAN
Sbjct: 241 DNSNAVVMVRYVSSLDNMRILMNLLRDPNKMIQRDAFHVFKLFVANKNKPLEITGVLVAN 300

Query: 301 RTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLSSSEMENCKIE 338
           RTKLLR LD+L+LDK +EGFEEDK QV+REIS+L  GDL +SE+E  +I+
Sbjct: 301 RTKLLRLLDELRLDKDDEGFEEDKAQVMREISMLETGDLWASEIEKDEIQ 350

[Close]
BLAST of Tan0012824 vs. ExPASy TrEMBL
Match: A0A6J1GKQ3 (putative MO25-like protein At5g47540 OS=Cucurbita moschata OX=3662 GN=LOC111454890 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 6.0e-154
Identity = 284/350 (81.14%), Postives = 305/350 (87.14%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGE 60
           M+GLFK K RSPVELVRYA+ELL+FIDRN+EVREQKREEKISELNK ISQMRTVLYGNG 
Sbjct: 1   MRGLFKPKARSPVELVRYANELLLFIDRNEEVREQKREEKISELNKTISQMRTVLYGNGG 60

Query: 61  TEPSPDACAQLTQEFFKENTFRLFIACIPRLNPG-------------RQQVKSRIIASEY 120
            EP+ DACAQLTQEFFKENTFRL + CIPRLN G             RQQ KSRIIASEY
Sbjct: 61  AEPNADACAQLTQEFFKENTFRLLVVCIPRLNSGLRQCATHVLANLQRQQFKSRIIASEY 120

Query: 121 LENNMDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFY 180
           LE NMD+MDILIPGYEDSDIALTYGAI RECI+HQCVAR VLESE++RKFFDYVQNPIFY
Sbjct: 121 LEKNMDIMDILIPGYEDSDIALTYGAIARECIQHQCVARYVLESENMRKFFDYVQNPIFY 180

Query: 181 VASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILL 240
           VASDA  TF+KLLTRHKSTVAAFLT NYDWFFE YNTQLLESTNYITKRHAVKLLGD+LL
Sbjct: 181 VASDASATFRKLLTRHKSTVAAFLTNNYDWFFEVYNTQLLESTNYITKRHAVKLLGDLLL 240

Query: 241 DNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVAN 300
           DNSNAAVMV+YVSSL+NMR+LMNLLRDPNK +QR+AFDVFKLFVANKNKP EI GILVAN
Sbjct: 241 DNSNAAVMVKYVSSLENMRVLMNLLRDPNKTIQRDAFDVFKLFVANKNKPPEIIGILVAN 300

Query: 301 RTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLSSSEMENCKIE 338
           RTKLL FLD LKLDK +EGFEEDK  +IREISI    D SS +MENC+ E
Sbjct: 301 RTKLLLFLDGLKLDKVDEGFEEDKALIIREISIYESFDPSSMKMENCETE 350

[Close]
BLAST of Tan0012824 vs. ExPASy TrEMBL
Match: A0A6J1I1R3 (putative MO25-like protein At5g47540 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468236 PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.3e-153
Identity = 281/350 (80.29%), Postives = 304/350 (86.86%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGE 60
           M+GLFK K RSPVELVRYA+ELL+FIDRN+EVREQKREEKISELNK ISQMRTVLYGNG 
Sbjct: 1   MRGLFKQKARSPVELVRYANELLLFIDRNEEVREQKREEKISELNKTISQMRTVLYGNGG 60

Query: 61  TEPSPDACAQLTQEFFKENTFRLFIACIPRLNPG-------------RQQVKSRIIASEY 120
            EP+ DACAQLTQEFFKENTFRL + CIPRLN G             RQQVKSRIIASEY
Sbjct: 61  AEPNADACAQLTQEFFKENTFRLLVVCIPRLNSGLRQCATHVLANLQRQQVKSRIIASEY 120

Query: 121 LENNMDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFY 180
           LENNMD+MDILIPGYEDSDIALTYG I RECI+HQCVAR VLESE++RKFFDYVQNPIFY
Sbjct: 121 LENNMDIMDILIPGYEDSDIALTYGVIARECIQHQCVARYVLESENMRKFFDYVQNPIFY 180

Query: 181 VASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILL 240
           VASDA  TF+KLLTRHKSTV  FLT NYDWFFE YN QLLESTNYITKRHAVKLLGD+LL
Sbjct: 181 VASDASATFRKLLTRHKSTVVGFLTNNYDWFFEVYNKQLLESTNYITKRHAVKLLGDLLL 240

Query: 241 DNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVAN 300
           DNSNAAVMV+YVSSL+NMR++MNLLRDPNK +QR+AFDVFKLFVANKNKP EI GIL+AN
Sbjct: 241 DNSNAAVMVKYVSSLENMRVMMNLLRDPNKTIQRDAFDVFKLFVANKNKPPEIIGILIAN 300

Query: 301 RTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLSSSEMENCKIE 338
           RTKLL FLD LKLDK +EGFEEDK  +IREISI    D SS +MENC+IE
Sbjct: 301 RTKLLLFLDGLKLDKVDEGFEEDKALIIREISIYESFDPSSMKMENCEIE 350

[Close]
BLAST of Tan0012824 vs. ExPASy TrEMBL
Match: A0A0A0K378 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G033420 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 6.7e-153
Identity = 276/350 (78.86%), Postives = 304/350 (86.86%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGE 60
           MKGLFK K RSPVELVRYA ELL+FIDRN+EVREQKR EKISELNK ISQMRT+LYG  +
Sbjct: 89  MKGLFKPKIRSPVELVRYAQELLLFIDRNEEVREQKRAEKISELNKTISQMRTILYGEAD 148

Query: 61  TEPSPDACAQLTQEFFKENTFRLFIACIPRLNPG-------------RQQVKSRIIASEY 120
            EP+PDAC+QLTQEFFKE+ FRLF+ CIP+LN G             RQQVKSRIIASEY
Sbjct: 149 AEPNPDACSQLTQEFFKEDMFRLFVTCIPKLNSGLRQCATHVLANLQRQQVKSRIIASEY 208

Query: 121 LENNMDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFY 180
            ENNMD+MDILIPGYEDSDIA+TYG+I RECIRHQCVA+ VLESEH+RKFFDY+QNPIFY
Sbjct: 209 FENNMDIMDILIPGYEDSDIAITYGSIARECIRHQCVAKYVLESEHIRKFFDYIQNPIFY 268

Query: 181 VASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILL 240
           VASDA  TF+KLLTRHKSTVA F TKN+DWFF+EYN +LLESTNYITKR  VKLLGDILL
Sbjct: 269 VASDASATFRKLLTRHKSTVAGFFTKNFDWFFKEYNMKLLESTNYITKRQGVKLLGDILL 328

Query: 241 DNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVAN 300
           DNSN+AVMV+YVSSLDNMRILMNLLRDPNK +QR+AFDVFKLFVANKNKP EIT +LVAN
Sbjct: 329 DNSNSAVMVQYVSSLDNMRILMNLLRDPNKAIQRDAFDVFKLFVANKNKPPEITSVLVAN 388

Query: 301 RTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLSSSEMENCKIE 338
           RTKLLRFLDDLK +KANEGFEEDK ++ R+ISIL   D SSSE ENC IE
Sbjct: 389 RTKLLRFLDDLKPEKANEGFEEDKAEITRQISILESSDPSSSETENCDIE 438

[Close]
BLAST of Tan0012824 vs. TAIR 10
Match: AT5G47540.1 (Mo25 family protein )

HSP 1 Score: 418.3 bits (1074), Expect = 5.8e-117
Identity = 213/343 (62.10%), Postives = 271/343 (79.01%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRN---DEVREQKREEKISELNKIISQMRTVLYG 60
           MKGLFK+KPR+P +LVR   +LL+F DR+    ++R+ KR+EK++EL++ I  M+++LYG
Sbjct: 1   MKGLFKSKPRTPADLVRQTRDLLLFSDRSTSLPDLRDSKRDEKMAELSRNIRDMKSILYG 60

Query: 61  NGETEPSPDACAQLTQEFFKENTFRLFIACIPRL-------------NPGRQQVKSRIIA 120
           N E EP  +ACAQLTQEFFKE+T RL I C+P+L             N  RQQV SR+IA
Sbjct: 61  NSEAEPVAEACAQLTQEFFKEDTLRLLITCLPKLNLETRKDATQVVANLQRQQVNSRLIA 120

Query: 121 SEYLENNMDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNP 180
           S+YLE N+D+MD+LI G+E++D+AL YGA+ RECIRHQ VA+ VLES+HV+KFFDY+Q P
Sbjct: 121 SDYLEANIDLMDVLIEGFENTDMALHYGAMFRECIRHQIVAKYVLESDHVKKFFDYIQLP 180

Query: 181 IFYVASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGD 240
            F +A+DA  TFK+LLTRHKSTVA FLTKN DWFF +YN++LLES+NYIT+R A+KLLGD
Sbjct: 181 NFDIAADAAATFKELLTRHKSTVAEFLTKNEDWFFADYNSKLLESSNYITRRQAIKLLGD 240

Query: 241 ILLDNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGIL 300
           ILLD SN+AVM +YVSS DN+RILMNLLR+ +K +Q EAF VFKLF AN+NKP +I  IL
Sbjct: 241 ILLDRSNSAVMTKYVSSRDNLRILMNLLRESSKSIQIEAFHVFKLFAANQNKPADIVNIL 300

Query: 301 VANRTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLS 328
           VANR+KLLR L DLK DK +E FE DK+QV+REI+ L   DL+
Sbjct: 301 VANRSKLLRLLADLKPDKEDERFEADKSQVLREIAALEPRDLA 343

[Close]
BLAST of Tan0012824 vs. TAIR 10
Match: AT4G17270.1 (Mo25 family protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.3e-111
Identity = 203/343 (59.18%), Postives = 264/343 (76.97%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRND---EVREQKREEKISELNKIISQMRTVLYG 60
           M+GLFK+KPR+P ++VR   +LL++ DR++   ++RE KREEK+ EL+K I  ++ +LYG
Sbjct: 1   MRGLFKSKPRTPADIVRQTRDLLLYADRSNSFPDLRESKREEKMVELSKSIRDLKLILYG 60

Query: 61  NGETEPSPDACAQLTQEFFKENTFRLFIACIPRL-------------NPGRQQVKSRIIA 120
           N E EP  +ACAQLTQEFFK +T R  +  +P L             N  RQQV SR+IA
Sbjct: 61  NSEAEPVAEACAQLTQEFFKADTLRRLLTSLPNLNLEARKDATQVVANLQRQQVNSRLIA 120

Query: 121 SEYLENNMDVMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNP 180
           ++YLE+N+D+MD L+ G+E++D+AL YG + RECIRHQ VA+ VL+SEHV+KFF Y+Q P
Sbjct: 121 ADYLESNIDLMDFLVDGFENTDMALHYGTMFRECIRHQIVAKYVLDSEHVKKFFYYIQLP 180

Query: 181 IFYVASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGD 240
            F +A+DA  TFK+LLTRHKSTVA FL KN DWFF +YN++LLESTNYIT+R A+KLLGD
Sbjct: 181 NFDIAADAAATFKELLTRHKSTVAEFLIKNEDWFFADYNSKLLESTNYITRRQAIKLLGD 240

Query: 241 ILLDNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGIL 300
           ILLD SN+AVM +YVSS+DN+RILMNLLR+ +K +Q EAF VFKLFVAN+NKP +I  IL
Sbjct: 241 ILLDRSNSAVMTKYVSSMDNLRILMNLLRESSKTIQIEAFHVFKLFVANQNKPSDIANIL 300

Query: 301 VANRTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLS 328
           VANR KLLR L D+K DK +E F+ DK QV+REI+ L L +L+
Sbjct: 301 VANRNKLLRLLADIKPDKEDERFDADKAQVVREIANLKLRELA 343

[Close]
BLAST of Tan0012824 vs. TAIR 10
Match: AT2G03410.1 (Mo25 family protein )

HSP 1 Score: 356.3 bits (913), Expect = 2.7e-98
Identity = 186/347 (53.60%), Postives = 247/347 (71.18%), Query Frame = 0 

Query: 1   MKGLFKTKPRSPVELVRYAHELLVFIDRNDE---VREQKREEKISELNKIISQMRTVLYG 60
           MKGLFK K R P E+VR   +L+   +  +E    R  KR    +EL + I  ++++LYG
Sbjct: 1   MKGLFKNKSRLPGEIVRQTRDLIALAESEEEETDARNSKRLGICAELCRNIRDLKSILYG 60

Query: 61  NGETEPSPDACAQLTQEFFKENTFRLFIACIPRL-------------NPGRQQVKSRIIA 120
           NGE EP P+AC  LTQEFF+ +T R  I  IP+L             N  +QQV+ R++A
Sbjct: 61  NGEAEPVPEACLLLTQEFFRADTLRPLIKSIPKLDLEARKDATQIVANLQKQQVEFRLVA 120

Query: 121 SEYLENNMDVMDILIPGYE-DSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQN 180
           SEYLE+N+DV+D L+ G + D ++AL Y  +L+EC+RHQ VA+ +LES+++ KFFDYVQ 
Sbjct: 121 SEYLESNLDVIDSLVEGIDHDHELALHYTGMLKECVRHQVVAKYILESKNLEKFFDYVQL 180

Query: 181 PIFYVASDAFVTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLG 240
           P F VA+DA   F++LLTRHKSTVA +L KNY+WFF EYNT+LLE  +Y TKR A KLLG
Sbjct: 181 PYFDVATDASKIFRELLTRHKSTVAEYLAKNYEWFFAEYNTKLLEKGSYFTKRQASKLLG 240

Query: 241 DILLDNSNAAVMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGI 300
           D+L+D SN+ VMV+YVSSLDN+RI+MNLLR+P K +Q EAF +FKLFVAN+NKP +I  I
Sbjct: 241 DVLMDRSNSGVMVKYVSSLDNLRIMMNLLREPTKNIQLEAFHIFKLFVANENKPEDIVAI 300

Query: 301 LVANRTKLLRFLDDLKLDKANEGFEEDKTQVIREISILVLGDLSSSE 331
           LVANRTK+LR   DLK +K + GFE DK  V+ EI+ L L D+ +++
Sbjct: 301 LVANRTKILRLFADLKPEKEDVGFETDKALVMNEIATLSLLDIKTAD 347

[Close]
BLAST of Tan0012824 vs. TAIR 10
Match: AT5G18940.2 (Mo25 family protein )

HSP 1 Score: 224.9 bits (572), Expect = 9.4e-59
Identity = 126/328 (38.41%), Postives = 195/328 (59.45%), Query Frame = 0 

Query: 7   TKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGETEPSPD 66
           ++P++P E+V+   + L+ +D    V  +  E+ + E+ K  S +R +L G+GETEP+ D
Sbjct: 9   SRPKTPQEVVKAIRDSLMALDTKTVVEVKALEKALEEVEKNFSSLRGILSGDGETEPNAD 68

Query: 67  ACAQLTQEFFKENTFRLFIACIPRLNPG-------------RQQVKSRIIASEYLENNMD 126
              QL  EF KE+   L I  +  L                +Q+V       +Y E + +
Sbjct: 69  QAVQLALEFCKEDVVSLVIHKLHILGWETRKDLLHCWSILLKQKVGDTYCCVQYFEEHFE 128

Query: 127 VMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFYVASDAF 186
           ++D L+  Y++ +IAL  G++LRECI+   +A+ +LES     FF +V+ P F VASDAF
Sbjct: 129 LLDSLVVCYDNKEIALHCGSMLRECIKFPSLAKYILESACFELFFKFVELPNFDVASDAF 188

Query: 187 VTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILLDNSNAA 246
            TFK LLT+H S V+ FLT +Y  FF+ Y  +LL S+NY+T+R ++KLL D LL+  N+ 
Sbjct: 189 STFKDLLTKHDSVVSEFLTSHYTEFFDVYE-RLLTSSNYVTRRQSLKLLSDFLLEPPNSH 248

Query: 247 VMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVANRTKLLR 306
           +M +++  +  ++++M LL+D +K +Q  AF +FK+FVAN NKP E+  IL  N  KLL 
Sbjct: 249 IMKKFILEVRYLKVIMTLLKDSSKNIQISAFHIFKIFVANPNKPQEVKIILARNHEKLLE 308

Query: 307 FLDDLKLDKANEGFEEDKTQVIREISIL 322
            L DL     ++ FEE+K  +I EI  L
Sbjct: 309 LLHDLSPGSEDDQFEEEKELIIEEIQKL 335

[Close]
BLAST of Tan0012824 vs. TAIR 10
Match: AT5G18940.1 (Mo25 family protein )

HSP 1 Score: 223.8 bits (569), Expect = 2.1e-58
Identity = 128/330 (38.79%), Postives = 196/330 (59.39%), Query Frame = 0 

Query: 7   TKPRSPVELVRYAHELLVFIDRNDEVREQKREEKISELNKIISQMRTVLYGNGETEPSPD 66
           ++P++P E+V+   + L+ +D    V  +  E+ + E+ K  S +R +L G+GETEP+ D
Sbjct: 9   SRPKTPQEVVKAIRDSLMALDTKTVVEVKALEKALEEVEKNFSSLRGILSGDGETEPNAD 68

Query: 67  ACAQLTQEFFKENTFRLFIACIPRLNPG-------------RQQVKSRIIASEYLENNMD 126
              QL  EF KE+   L I  +  L                +Q+V       +Y E + +
Sbjct: 69  QAVQLALEFCKEDVVSLVIHKLHILGWETRKDLLHCWSILLKQKVGDTYCCVQYFEEHFE 128

Query: 127 VMDILIPGYEDSDIALTYGAILRECIRHQCVARCVLESEHVRKFFDYVQNPIFYVASDAF 186
           ++D L+  Y++ +IAL  G++LRECI+   +A+ +LES     FF +V+ P F VASDAF
Sbjct: 129 LLDSLVVCYDNKEIALHCGSMLRECIKFPSLAKYILESACFELFFKFVELPNFDVASDAF 188

Query: 187 VTFKKLLTRHKSTVAAFLTKNYDWFFEEYNTQLLESTNYITKRHAVKLLGDILLDNSNAA 246
            TFK LLT+H S V+ FLT +Y  FF+ Y  +LL S+NY+T+R ++KLL D LL+  N+ 
Sbjct: 189 STFKDLLTKHDSVVSEFLTSHYTEFFDVYE-RLLTSSNYVTRRQSLKLLSDFLLEPPNSH 248

Query: 247 VMVRYVSSLDNMRILMNLLRDPNKMLQREAFDVFKLFVANKNKPLEITGILVANRTKLLR 306
           +M +++  +  ++++M LL+D +K +Q  AF +FK+FVAN NKP E+  IL  N  KLL 
Sbjct: 249 IMKKFILEVRYLKVIMTLLKDSSKNIQISAFHIFKIFVANPNKPQEVKIILARNHEKLLE 308

Query: 307 FLDDLKLDKANEG--FEEDKTQVIREISIL 322
            L DL   K +E   FEE+K  +I EI  L
Sbjct: 309 LLHDLSPGKGSEDDQFEEEKELIIEEIQKL 337

The following BLAST results are available for this feature:
BLAST of Tan0012824 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FGK38.2e-11662.10Putative MO25-like protein At5g47540 OS=Arabidopsis thaliana OX=3702 GN=At5g4754... [more]
Q9M0M41.8e-11059.18Putative MO25-like protein At4g17270 OS=Arabidopsis thaliana OX=3702 GN=At4g1727... [more]
Q9ZQ773.8e-9753.60MO25-like protein At2g03410 OS=Arabidopsis thaliana OX=3702 GN=At2g03410 PE=2 SV... [more]
Q061384.7e-6339.94Calcium-binding protein 39 OS=Mus musculus OX=10090 GN=Cab39 PE=1 SV=2[more]
Q29RI61.4e-6239.63Calcium-binding protein 39 OS=Bos taurus OX=9913 GN=CAB39 PE=2 SV=1[more]
BLAST of Tan0012824 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038887041.12.1e-16187.69putative MO25-like protein At5g47540 isoform X2 [Benincasa hispida][more]
XP_038887040.11.3e-15884.39putative MO25-like protein At5g47540 isoform X1 [Benincasa hispida][more]
KAG6589115.11.1e-15781.14putative MO25-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022989431.11.9e-15781.43putative MO25-like protein At5g47540 [Cucurbita maxima][more]
XP_022928128.11.9e-15781.14putative MO25-like protein At5g47540 [Cucurbita moschata][more]
BLAST of Tan0012824 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JK199.0e-15881.43putative MO25-like protein At5g47540 OS=Cucurbita maxima OX=3661 GN=LOC111486487... [more]
A0A6J1EN089.0e-15881.14putative MO25-like protein At5g47540 OS=Cucurbita moschata OX=3662 GN=LOC1114350... [more]
A0A6J1GKQ36.0e-15481.14putative MO25-like protein At5g47540 OS=Cucurbita moschata OX=3662 GN=LOC1114548... [more]
A0A6J1I1R32.3e-15380.29putative MO25-like protein At5g47540 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A0A0K3786.7e-15378.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G033420 PE=3 SV=1[more]
BLAST of Tan0012824 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G47540.15.8e-11762.10Mo25 family protein [more]
AT4G17270.11.3e-11159.18Mo25 family protein [more]
AT2G03410.12.7e-9853.60Mo25 family protein [more]
AT5G18940.29.4e-5938.41Mo25 family protein [more]
AT5G18940.12.1e-5838.79Mo25 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013878Mo25-likePFAMPF08569Mo25coord: 4..321
e-value: 2.6E-104
score: 349.1
IPR013878Mo25-likePANTHERPTHR10182CALCIUM-BINDING PROTEIN 39-RELATEDcoord: 1..336
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 3..321
e-value: 2.7E-112
score: 377.2
NoneNo IPR availablePANTHERPTHR10182:SF17PROTEIN, PUTATIVE-RELATEDcoord: 1..336
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 10..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0012824.1Tan0012824.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0071902 positive regulation of protein serine/threonine kinase activity
molecular_function GO:0043539 protein serine/threonine kinase activator activity