Tan0012704 (gene) Snake gourd v1

Overview
NameTan0012704
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptioncytochrome P450 CYP82D47-like
LocationLG03: 76624428 .. 76626009 (+)
RNA-Seq ExpressionTan0012704
SyntenyTan0012704
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAAGAGTGGCCAACGGCGGCGAAGTAAAGGGTAAAAATGATCGAAAGCCTCCGACAGTAGCCGGCGGATGGCCTCTGATTGGGCACCTCCCTTTGTTCGGAGGATCGGAACCGCCCCATATAAAACTGAGTGATTTGGCGGATAAATACGGTTCAATCTTCTCCATTCGACTGGGTATCCACTCAGCAGTAGTGATAAACAATTGGGAGGGCGCAAAAGAGTGCTTCACCACCCACGATGCCGCCGTATTTTCCCGACCCAAATTACTCAGCGGAAAACTCATGGGCTACAACTACTCCATGTTCATTTTCAGTCCCTACAACTCTTACTGGCGCAAAATGCGTAAAATAACCACACTCGAGCTTCTATCCAACCGCAGGCTCAAGCTCCTAAGACACGTTCGAGAGACAGAGGTAAACCAAGCCATAAAAGAGCTGTACCAAGAAAAAGAGAGGGTGGTTTGGTGGACATGAAGCGCTGGTTTAGGGACTTGATTATGAACTTGGTTCTGTTAGTGGTGGCTGGGAAAAGGTACTTTGGAGTGGCAACAACGGTAGAGGCGCGACGGTGGCAGAACGCAATCGAGGAGTCGTTGCGTTTGTTGGGAATTTATCATGTGTTGGGCGATGCTATTCCGTGTTTAGAATGGTTGGATGTTGGCGGCCATGTGAAGGCCATGAAGACAACTTCAAAGGTGTTGGATGATATGTTGACGCAGTGGTTGGAGGAGCATAAGAAGGACAGAGCTTCTGCTGAAATTGCCAAAAAGGATACAGATTTCATGGATGTGATGCTTTCAGTTCTTCACGAAACAGGCGCTGATGCTGCCCATGGCTTCGATCACGATACCGTTATCAAATCTACTTGTTTGGTACCAATTCTATTTTCCATCACGTTTCGTCTATTATTTATCATAGAATATTCTAAACTTGTTATACAAAAATGATGTATGTATAGACGATGGTTTTGGGAGGGAGTGAGACAACCGTGGTGGCAGTGATATGGGCACTCTCGTTACTATTGAACAATCGCCATGCATTGAGAAAAGTGCAAGAAGAACTTGACACCCACATAGGTCGAGATACAAAACTGAAAGAATCAGATATCGAAAAGCTAACTTATTTGGCGGCAGTAGTAAAAGAGGCAATGCGACTATATGGAGCAGGAAGCCTCATCTCTCGAGAAGTGGCGGAGGATCTCACCATCGATGGCTTCCATATCAAGAAAGGGACATGGTTGTTCATCAACTTGTGGAAGATCCACAGAGATGAGAGAGTATGGGCGGAGCCATTGGTGTTTAAACCAGAGAGGTTTTTGACGACCCATAAGGATTTTGATGTGAAGGGCCACCAATTTGAACTCATTCCTTTTGGAGGAGGAAGAAGAGTTTGTCCTGGCTTGTCTTTTGGGCTCCAATTGGTACAACTTATTTTGGGCAGGTTGCTTCATGCTTTCGATATATGGACACCGGAGGACGCTGCTGTTGATATGACTGGAACAATTGGAATGACGAACATGAAAGCTACCCCTTTGGAAGTACTCATCAAACCACGCTTGTCTCCCATGCTTTATGATTGA

mRNA sequence

ATGAAAAGAGTGGCCAACGGCGGCGAAGTAAAGGGTAAAAATGATCGAAAGCCTCCGACAGTAGCCGGCGGATGGCCTCTGATTGGGCACCTCCCTTTGTTCGGAGGATCGGAACCGCCCCATATAAAACTGAGTGATTTGGCGGATAAATACGGTTCAATCTTCTCCATTCGACTGGGTATCCACTCAGCAGTAGTGATAAACAATTGGGAGGGCGCAAAAGAGTGCTTCACCACCCACGATGCCGCCGTATTTTCCCGACCCAAATTACTCAGCGGAAAACTCATGGGCTACAACTACTCCATGTTCATTTTCAGTCCCTACAACTCTTACTGGCGCAAAATGCGTAAAATAACCACACTCGAGCTTCTATCCAACCGCAGGCTCAAGCTCCTAAGACACGTTCGAGAGACAGAGGGTGGTTTGGTGGACATGAAGCGCTGGTTTAGGGACTTGATTATGAACTTGGTTCTGTTAGTGGTGGCTGGGAAAAGGTACTTTGGAGTGGCAACAACGGTAGAGGCGCGACGGTGGCAGAACGCAATCGAGGAGTCGTTGCGTTTGTTGGGAATTTATCATGTGTTGGGCGATGCTATTCCGTGTTTAGAATGGTTGGATGTTGGCGGCCATGTGAAGGCCATGAAGACAACTTCAAAGGTGTTGGATGATATGTTGACGCAGTGGTTGGAGGAGCATAAGAAGGACAGAGCTTCTGCTGAAATTGCCAAAAAGGATACAGATTTCATGGATGTGATGCTTTCAGTTCTTCACGAAACAGGCGCTGATGCTGCCCATGGCTTCGATCACGATACCGTTATCAAATCTACTTGTTTGACGATGGTTTTGGGAGGGAGTGAGACAACCGTGGTGGCAGTGATATGGGCACTCTCGTTACTATTGAACAATCGCCATGCATTGAGAAAAGTGCAAGAAGAACTTGACACCCACATAGGTCGAGATACAAAACTGAAAGAATCAGATATCGAAAAGCTAACTTATTTGGCGGCAGTAGTAAAAGAGGCAATGCGACTATATGGAGCAGGAAGCCTCATCTCTCGAGAAGTGGCGGAGGATCTCACCATCGATGGCTTCCATATCAAGAAAGGGACATGGTTGTTCATCAACTTGTGGAAGATCCACAGAGATGAGAGAGTATGGGCGGAGCCATTGGTGTTTAAACCAGAGAGGTTTTTGACGACCCATAAGGATTTTGATGTGAAGGGCCACCAATTTGAACTCATTCCTTTTGGAGGAGGAAGAAGAGTTTGTCCTGGCTTGTCTTTTGGGCTCCAATTGGTACAACTTATTTTGGGCAGGTTGCTTCATGCTTTCGATATATGGACACCGGAGGACGCTGCTGTTGATATGACTGGAACAATTGGAATGACGAACATGAAAGCTACCCCTTTGGAAGTACTCATCAAACCACGCTTGTCTCCCATGCTTTATGATTGA

Coding sequence (CDS)

ATGAAAAGAGTGGCCAACGGCGGCGAAGTAAAGGGTAAAAATGATCGAAAGCCTCCGACAGTAGCCGGCGGATGGCCTCTGATTGGGCACCTCCCTTTGTTCGGAGGATCGGAACCGCCCCATATAAAACTGAGTGATTTGGCGGATAAATACGGTTCAATCTTCTCCATTCGACTGGGTATCCACTCAGCAGTAGTGATAAACAATTGGGAGGGCGCAAAAGAGTGCTTCACCACCCACGATGCCGCCGTATTTTCCCGACCCAAATTACTCAGCGGAAAACTCATGGGCTACAACTACTCCATGTTCATTTTCAGTCCCTACAACTCTTACTGGCGCAAAATGCGTAAAATAACCACACTCGAGCTTCTATCCAACCGCAGGCTCAAGCTCCTAAGACACGTTCGAGAGACAGAGGGTGGTTTGGTGGACATGAAGCGCTGGTTTAGGGACTTGATTATGAACTTGGTTCTGTTAGTGGTGGCTGGGAAAAGGTACTTTGGAGTGGCAACAACGGTAGAGGCGCGACGGTGGCAGAACGCAATCGAGGAGTCGTTGCGTTTGTTGGGAATTTATCATGTGTTGGGCGATGCTATTCCGTGTTTAGAATGGTTGGATGTTGGCGGCCATGTGAAGGCCATGAAGACAACTTCAAAGGTGTTGGATGATATGTTGACGCAGTGGTTGGAGGAGCATAAGAAGGACAGAGCTTCTGCTGAAATTGCCAAAAAGGATACAGATTTCATGGATGTGATGCTTTCAGTTCTTCACGAAACAGGCGCTGATGCTGCCCATGGCTTCGATCACGATACCGTTATCAAATCTACTTGTTTGACGATGGTTTTGGGAGGGAGTGAGACAACCGTGGTGGCAGTGATATGGGCACTCTCGTTACTATTGAACAATCGCCATGCATTGAGAAAAGTGCAAGAAGAACTTGACACCCACATAGGTCGAGATACAAAACTGAAAGAATCAGATATCGAAAAGCTAACTTATTTGGCGGCAGTAGTAAAAGAGGCAATGCGACTATATGGAGCAGGAAGCCTCATCTCTCGAGAAGTGGCGGAGGATCTCACCATCGATGGCTTCCATATCAAGAAAGGGACATGGTTGTTCATCAACTTGTGGAAGATCCACAGAGATGAGAGAGTATGGGCGGAGCCATTGGTGTTTAAACCAGAGAGGTTTTTGACGACCCATAAGGATTTTGATGTGAAGGGCCACCAATTTGAACTCATTCCTTTTGGAGGAGGAAGAAGAGTTTGTCCTGGCTTGTCTTTTGGGCTCCAATTGGTACAACTTATTTTGGGCAGGTTGCTTCATGCTTTCGATATATGGACACCGGAGGACGCTGCTGTTGATATGACTGGAACAATTGGAATGACGAACATGAAAGCTACCCCTTTGGAAGTACTCATCAAACCACGCTTGTCTCCCATGCTTTATGATTGA

Protein sequence

MKRVANGGEVKGKNDRKPPTVAGGWPLIGHLPLFGGSEPPHIKLSDLADKYGSIFSIRLGIHSAVVINNWEGAKECFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLKLLRHVRETEGGLVDMKRWFRDLIMNLVLLVVAGKRYFGVATTVEARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQWLEEHKKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTCLTMVLGGSETTVVAVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGAGSLISREVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHKDFDVKGHQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLEVLIKPRLSPMLYD
Homology
BLAST of Tan0012704 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 2.2e-138
Identity = 248/499 (49.70%), Postives = 327/499 (65.53%), Query Frame = 0

Query: 11  KGKNDRKPPTVAGGWPLIGHLPLFGGSEPPHIKLSDLADKYGSIFSIRLGIHSAVVINNW 70
           K    +  P V+G WP++GHL L  GS+ PH  L  LADKYG +F+I+LG+  A+V++NW
Sbjct: 31  KNSRGKDAPVVSGAWPILGHLSLLNGSQTPHKVLGALADKYGPLFTIKLGMKPALVLSNW 90

Query: 71  EGAKECFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLK 130
           E +KE FTT+D AV SRPKL++ ++M YN +    +PY  YWR++RKI T E LSNRR++
Sbjct: 91  EMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVGLAPYGPYWRELRKIVTFEFLSNRRIE 150

Query: 131 LLRHVR---------------------ETEGGLVDMKRWFRDLIMNLVLLVVAGKRYFGV 190
              H+R                     E+   LVD+K+W   L  N+V+ +V GKRYFGV
Sbjct: 151 QRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLVDIKQWLAYLTFNMVVRMVVGKRYFGV 210

Query: 191 ATTV---EARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLT 250
                  +A+R+   I E + L+G + V  D +PCL WLD+GGH KAMK  +K +D +L+
Sbjct: 211 MHVEGKDKAQRFMKNIREFMNLMGTFTV-ADGVPCLRWLDLGGHEKAMKANAKEVDKLLS 270

Query: 251 QWLEEHKKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTCLTMVLGGSE 310
           +WLEEH++ +   E  + D DFMDVM+S L+  GA     FD DT+ K+T L ++LGG++
Sbjct: 271 EWLEEHRQKKLLGENVESDRDFMDVMISALN--GAQIG-AFDADTICKATSLELILGGTD 330

Query: 311 TTVVAVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYG 370
           +T V + WALSLLL N  AL K +EE+D  IG+D  ++ESDI KL YL A+VKE +RLY 
Sbjct: 331 STAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYIRESDISKLVYLQAIVKETLRLYP 390

Query: 371 AGSLIS-REVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHKDFD 430
                S RE  E+  + G+HIKKGT L  NLWKIHRD  VW++PL FKPERFLTTHKD D
Sbjct: 391 PAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRDPSVWSDPLEFKPERFLTTHKDVD 450

Query: 431 VKGHQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGMTNM 485
           ++GH FEL+PFG GRRVC G+S GL +V   L  LLH+FDI  P    VDMT   G TN 
Sbjct: 451 LRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLLHSFDILNPSAEPVDMTEFFGFTNT 510

BLAST of Tan0012704 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 6.6e-135
Identity = 246/492 (50.00%), Postives = 326/492 (66.26%), Query Frame = 0

Query: 14  NDRKPPTVAGGWPLIGHLPLFGGSEPPHIKLSDLADKYGSIFSIRLGIHSAVVINNWEGA 73
           + ++ PTV G WP+ GHLPL  GS+ PH  L  LA+K+G +F+I+LG   A+V+++WE A
Sbjct: 36  SSKEAPTVGGAWPIFGHLPLLIGSKSPHKALGALAEKHGPLFTIKLGAKKALVVSDWEMA 95

Query: 74  KECFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLKLLR 133
           +ECFTT+D AV +RPKLL  +LM YN +M + +PY  YWR++RKI   E+LS+ R++ L+
Sbjct: 96  RECFTTNDVAVSARPKLLVAELMCYNNAMLLVAPYGPYWRELRKIIVTEILSSSRVEQLQ 155

Query: 134 HVR--------------------ETEGGLVDMKRWFRDLIMNLVLLVVAGKRYF-GVATT 193
            VR                    E++   V++K+WF   I N+VL +V GKR+    AT 
Sbjct: 156 DVRVSEVQNSIVELYDVWRSQKNESDYASVELKQWFAQPIFNMVLRMVVGKRFLSATATD 215

Query: 194 VEARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQWLEEH 253
            +A +   A++E +RL G++ V GDAIP L WLD GG+ KAMK T+K LD M+++WLEEH
Sbjct: 216 EKAEKCVKAVDEFMRLAGVFTV-GDAIPYLRWLDFGGYEKAMKETAKELDVMISEWLEEH 275

Query: 254 KKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTCLTMVLGGSETTVVAV 313
           ++ RA  E      DFM+VMLS L     D   G D DT+IKST LT++  G+E ++  +
Sbjct: 276 RQKRALGEGVDGAQDFMNVMLSSLDGKTID---GIDADTLIKSTVLTIIQAGTEASISTI 335

Query: 314 IWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGAGSLIS 373
           IWA+ L+L N   L   + ELD  +G+D  + ESDI  L YL AVVKE +RLY  G L S
Sbjct: 336 IWAMCLILKNPLILEN-KAELDIQVGKDRCICESDISNLVYLQAVVKETLRLYAPGPLSS 395

Query: 374 -REVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHKDFDVKGHQF 433
            RE AED T+ G+H+KKGT L  N+WKIH D  VW++P  FKP+RFLTTHKD DVKGH F
Sbjct: 396 PREFAEDCTLGGYHVKKGTRLITNIWKIHTDPNVWSDPFEFKPDRFLTTHKDIDVKGHHF 455

Query: 434 ELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLE 484
           +L+PFG GRRVCPG+SFGLQ V L L   LH+F+I  P    +DMT   G+TN KATPLE
Sbjct: 456 QLLPFGSGRRVCPGISFGLQTVHLALASFLHSFEILNPSTEPLDMTEAFGVTNTKATPLE 515

BLAST of Tan0012704 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 473.4 bits (1217), Expect = 3.1e-132
Identity = 246/519 (47.40%), Postives = 330/519 (63.58%), Query Frame = 0

Query: 11  KGKNDRKPPTVAGGWPLIGHLPLFGGSEPPHIKLSDLADKYGSIFSIRLGIHSAVVINNW 70
           K  + ++PP ++G WPL+GHLPL   ++ PH  L  L DKYG IF+I+LG  +A+V++NW
Sbjct: 28  KVSHTKEPPIISGSWPLLGHLPLMRNTQTPHKTLGALVDKYGPIFTIKLGATNALVLSNW 87

Query: 71  EGAKECFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLK 130
           E AKECFT +D  V SRPK ++ +LM YN +   ++PY +YWR++RKI TLE+LSNRR++
Sbjct: 88  ELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPYGAYWRQLRKIVTLEILSNRRIE 147

Query: 131 LLRHVRETE-------------------GGLVD--------------------MKRWFRD 190
           LL H+R +E                    GL+D                    +K+WF  
Sbjct: 148 LLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDTKSSSTNDEPSTTDYVSVELKKWFAQ 207

Query: 191 LIMNLVLLVVAGKRYFG---VATTVEARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVG 250
           L +N+VL +V GKR FG   V    EA+R+   I + +RL+G + V GD +P L+WLD+G
Sbjct: 208 LTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENIRDFMRLIGTFTV-GDGVPFLKWLDLG 267

Query: 251 GHVKAMKTTSKVLDDMLTQWLEEH--KKDRASAEIAKKDTDFMDVMLSVLHETGADAAHG 310
           GH K MK  +K  D ML +WLEEH  KK   S +    + DFMD ML VL +   +   G
Sbjct: 268 GHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSEDKVVGERDFMDAMLLVLKDKPIE---G 327

Query: 311 FDHDTVIKSTCLTMVLGGSETTVVAVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKES 370
           FD DT+IK+T L ++LGGS+TT   + WA+ LLL + H L K++EEL+T+IG++  + ES
Sbjct: 328 FDVDTIIKATTLELILGGSDTTAGTLTWAMCLLLKHPHVLEKLKEELNTYIGKERCVNES 387

Query: 371 DIEKLTYLAAVVKEAMRLYGAGSLIS-REVAEDLTIDGFHIKKGTWLFINLWKIHRDERV 430
           DI KL YL A++KE +RLY      S RE  ED TI G+HIKKGT L  NLWKIHRD  V
Sbjct: 388 DINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIGGYHIKKGTRLMPNLWKIHRDPSV 447

Query: 431 WAEPLVFKPERFLTTHKDFDVKGHQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFD 485
           W +PL FKPERFL+THKD DV+G  FEL+PFG GRR+C G+S GL +V  IL   LH+F+
Sbjct: 448 WPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGRRMCAGMSLGLHMVHYILANFLHSFE 507

BLAST of Tan0012704 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 3.8e-130
Identity = 239/492 (48.58%), Postives = 314/492 (63.82%), Query Frame = 0

Query: 16  RKPPTVAGGWPLIGHLPLFGGSEPPHIKLSDLADKYGSIFSIRLGIHSAVVINNWEGAKE 75
           ++PP  AG WP+IGHL L    + PH  LS  ADK G +F I+LG+H A+V+NN E AKE
Sbjct: 34  KEPPQPAGAWPIIGHLHLLARGDLPHKILSSFADKNGPVFKIQLGVHQALVVNNSEIAKE 93

Query: 76  CFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLKLLRHV 135
           CFTT+D    +RP  ++ K+MGYNY M   +PY  YWR MRKI  LE LSNRRL+ L+HV
Sbjct: 94  CFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAPYGPYWRDMRKIIMLEFLSNRRLQSLKHV 153

Query: 136 RETEGG------------------LVDMKRWFRDLIMNLVLLVVAGKRYFGVATT----- 195
             +E                    LVDMK+W  DL +N+ + +V GKR+FG A+      
Sbjct: 154 WHSEISISSKELYKLWETQNIDFCLVDMKQWLADLTLNMSVKMVVGKRFFGSASASACEE 213

Query: 196 VEARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQWLEEH 255
            E+      +    RL+G + VL D +P L WLD+GGH K MK T K LD +   WL+EH
Sbjct: 214 TESSNCPKTLRNMFRLMGSF-VLSDYLPYLRWLDLGGHEKEMKRTVKELDILFKGWLDEH 273

Query: 256 KKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTCLTMVLGGSETTVVAV 315
           K+ R S      D DFMDVMLS+L E+      G D DT+ K+ CL ++LGG++TT   +
Sbjct: 274 KRKRLSGGKEDDDQDFMDVMLSILEESKL----GNDVDTINKTACLAIILGGADTTWATL 333

Query: 316 IWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGAGSLIS 375
            WALSLLLNN +AL+K Q+ELD H+GRD  + ESD+ KLTY+ A++KE +RLY  G L+ 
Sbjct: 334 TWALSLLLNNPNALKKAQDELDLHVGRDRNVDESDLVKLTYIDAIIKETLRLYPPGPLLG 393

Query: 376 -REVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHKDFDVKGHQF 435
            R V ED TI G+H++ GT L +N WKI RD  VW++P  ++PERFL   +D D+KG  F
Sbjct: 394 PRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDPLVWSQPHEYQPERFL--ERDVDMKGQHF 453

Query: 436 ELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLE 484
           ELIPFG GRR CP +S  LQ++ L L  +LH F++ TP    VDMT T G+ + KATPLE
Sbjct: 454 ELIPFGSGRRACPAISLALQVLPLTLAHILHGFELRTPNQNKVDMTETPGIVHAKATPLE 513

BLAST of Tan0012704 vs. ExPASy Swiss-Prot
Match: O81972 (Cytochrome P450 82A2 OS=Glycine max OX=3847 GN=CYP82A2 PE=2 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 8.4e-130
Identity = 243/502 (48.41%), Postives = 319/502 (63.55%), Query Frame = 0

Query: 7   GGEVKGKNDRKPPTVAGGWPLIGHLPLFGGSEPPHIKLSDLADKYGSIFSIRLGIHSAVV 66
           GG  K   +  PPTVAG WP+IGHLPL  GS+ PH  L DLADKYG IFSI++G  +AVV
Sbjct: 27  GGSWK-SGEEGPPTVAGAWPIIGHLPLLLGSKTPHKTLGDLADKYGPIFSIKIGAKNAVV 86

Query: 67  INNWEGAKECFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSN 126
           ++NWE AKEC+TT+D AV S P L+S  L+ YN SM + +PY  YWR++RKI   E LS 
Sbjct: 87  VSNWEMAKECYTTNDIAVSSLPDLISANLLCYNRSMIVVAPYGPYWRQLRKILMSEFLSP 146

Query: 127 RRLKLLRHVRETE---------------------GGLVDMKRWFRDLIMNLVLLVVAGKR 186
            R++ L HVR +E                        V++K+WF  L+ N++L +V GKR
Sbjct: 147 SRVEQLHHVRVSEVQSSITELFRDWRSNKNVQSGFATVELKQWFSLLVFNMILRMVCGKR 206

Query: 187 YFGVATT--VEARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDD 246
           YF  +T+   +A R   A++E +RL   + V GDAIP L W D GG+   M+ T K LD+
Sbjct: 207 YFSASTSDDEKANRCVKAVDEFVRLAATFTV-GDAIPYLRWFDFGGYENDMRETGKELDE 266

Query: 247 MLTQWLEEHKKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTCLTMVLG 306
           ++ +WL+EH++ R   E  +   D M V+LS+L         G + D VIKS  LT++  
Sbjct: 267 IIGEWLDEHRQKRKMGENVQ---DLMSVLLSLLE---GKTIEGMNVDIVIKSFVLTVIQA 326

Query: 307 GSETTVVAVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMR 366
           G+E ++  +IWA SL+LNN   L K++ ELD  +G++  + ESD+ KLTYL AVVKE +R
Sbjct: 327 GTEASITTLIWATSLILNNPSVLEKLKAELDIQVGKERYICESDLSKLTYLQAVVKETLR 386

Query: 367 LYGAGSLI-SREVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHK 426
           LY    L   RE  ED TI G+ +KKGT L  NL KIH D  VW+ PL FKPERFLTT K
Sbjct: 387 LYPPAPLSRPREFEEDCTIGGYTVKKGTRLITNLSKIHTDHNVWSNPLEFKPERFLTTDK 446

Query: 427 DFDVKGHQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGM 485
           D D+KG  F+L+PFGGGRR+CPG++ GLQ V+L L   LH+F+I  P    +DMT     
Sbjct: 447 DIDMKGQHFQLLPFGGGRRICPGINLGLQTVRLTLASFLHSFEILNPSTEPLDMTEVFRA 506

BLAST of Tan0012704 vs. NCBI nr
Match: XP_038883369.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 775.0 bits (2000), Expect = 3.8e-220
Identity = 394/501 (78.64%), Postives = 424/501 (84.63%), Query Frame = 0

Query: 1   MKRVANG-GEVKGKNDRKPPTVAGGWPLIGHLPLFGGSEPPHIKLSDLADKYGSIFSIRL 60
           MKRV N  G VKG+ + KPP V GGWPLI HLPLFGG EPPH+KLS LADKYG IFSI+L
Sbjct: 35  MKRVTNNTGGVKGRRNPKPPMVGGGWPLIRHLPLFGGPEPPHLKLSALADKYGPIFSIQL 94

Query: 61  GIHSAVVINNWEGAKECFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSP-YNSYWRKMRKI 120
           G HSAVVINNWE AK+CFTTHD AVF RPKLLSGKLMGYNY+MFIFSP YNSYWRKMRKI
Sbjct: 95  GTHSAVVINNWEAAKQCFTTHDIAVFPRPKLLSGKLMGYNYAMFIFSPYYNSYWRKMRKI 154

Query: 121 TTLELLSNRRLKLLRHVRETE-----------GGLVDMKRWFRDLIMNLVLLVVAGKRYF 180
           TTL+LLSNRRLKLLR VRE+E           GG V+MK+WF DLIMNLVLL+V GKRYF
Sbjct: 155 TTLDLLSNRRLKLLRQVRESEVNRAIKELYQLGGRVEMKQWFGDLIMNLVLLIVGGKRYF 214

Query: 181 GVATTV---EARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDM 240
           G    V   EA+R Q A+EESLRLLG+Y VLGDAIP L WLDVGGHVKAMK T KVLD M
Sbjct: 215 GAGAMVEEEEAQRCQKAMEESLRLLGMY-VLGDAIPYLGWLDVGGHVKAMKRTFKVLDGM 274

Query: 241 LTQWLEEHKKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTCLTMVLGG 300
           LTQWLEEHKK+RA     K + DFMDVMLSVL ET  D  HGF  DTVIK+TCLTMVLG 
Sbjct: 275 LTQWLEEHKKNRA----GKNNGDFMDVMLSVLEETDNDILHGFHRDTVIKATCLTMVLGA 334

Query: 301 SETTVVAVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRL 360
           SET++VAV+WALSLLLNNR  LRKVQEELDTHIGRD KL+ESD+EKL YL AVVKEAMRL
Sbjct: 335 SETSIVAVVWALSLLLNNRDTLRKVQEELDTHIGRDAKLQESDMEKLVYLEAVVKEAMRL 394

Query: 361 YGAGSLISREVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHKDF 420
           +GAGSLISRE AED+ +DGFHIKKGTWLFINLWKIHRDERVW EPL FKPERFLTTHK+F
Sbjct: 395 FGAGSLISREAAEDIDVDGFHIKKGTWLFINLWKIHRDERVWDEPLKFKPERFLTTHKNF 454

Query: 421 DVK-GHQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGMT 480
           DVK G QFELIPFGGGRR+CPGL FGLQLV+ +LG LLHAFDIWTPEDAAVDMTGTIGMT
Sbjct: 455 DVKGGQQFELIPFGGGRRICPGLWFGLQLVEFVLGGLLHAFDIWTPEDAAVDMTGTIGMT 514

Query: 481 NMKATPLEVLIKPRLSPMLYD 485
           NMKATPLEV IKPRLSPMLY+
Sbjct: 515 NMKATPLEVFIKPRLSPMLYE 530

BLAST of Tan0012704 vs. NCBI nr
Match: XP_022133138.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 618.2 bits (1593), Expect = 5.9e-173
Identity = 312/458 (68.12%), Postives = 357/458 (77.95%), Query Frame = 0

Query: 17  KPPTVAGGWPLIGHLPLFGGSEPPHIKLSDLADKYGSIFSIRLGIHSAVVINNWEGAKEC 76
           KPP+V GGWPLIGHLPL GG + PH+KL  LAD+YG IFSI+LGIH AVVIN+WE AK+C
Sbjct: 43  KPPSVPGGWPLIGHLPLLGGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQC 102

Query: 77  FTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLKLLRHVR 136
           FTTHD AV  RPK+L+ +++GYNY++F F PY+SYWRKMRK TTL LLS+RRL+LLR VR
Sbjct: 103 FTTHDVAVSFRPKILAAQILGYNYAVFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVR 162

Query: 137 ETE--------------------GGLVDMKRWFRDLIMNLVLLVVAGKRYFGVATTV--- 196
           E+E                     GLV+MK+W  DL MNL+L VVAGKRYFG    V   
Sbjct: 163 ESEVNQAIKELYQIWKERRDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEA 222

Query: 197 EARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQWLEEHK 256
           EAR  Q A++E LRL+G++ +LGDA+P L WLDVGGHVKAMK TSK LD +LTQWLEEHK
Sbjct: 223 EARACQKAMKEFLRLMGLF-ILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHK 282

Query: 257 KDRASAEIAKKDTDFMDVMLSVLHET-----GADAAHGFDHDTVIKSTCLTMVLGGSETT 316
           K RAS + +K+D DFMDVMLS L ET      +D+ H FD DT+IK+TCLTMVLGGS+TT
Sbjct: 283 KKRASGK-SKEDKDFMDVMLSTLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTT 342

Query: 317 VVAVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGAG 376
            V V WALSLLLNN  AL KVQEE+D HIGRD +LKESD+ +L YL AVVKE MRLYGAG
Sbjct: 343 TVVVTWALSLLLNNHPALIKVQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAG 402

Query: 377 SLISREVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHKDFDVKG 436
            LI RE +ED  IDGFHI+KGT LFIN WKIHRD RVW EPL FKPERFLTTH+DFDV G
Sbjct: 403 GLILRETSEDFAIDGFHIEKGTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNG 462

Query: 437 HQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDI 447
            QFELIPFGGGRR CPGLSFGLQ+VQLILG LLHAFDI
Sbjct: 463 QQFELIPFGGGRRACPGLSFGLQMVQLILGALLHAFDI 498

BLAST of Tan0012704 vs. NCBI nr
Match: KAG2663852.1 (hypothetical protein I3760_16G051800 [Carya illinoinensis])

HSP 1 Score: 581.3 bits (1497), Expect = 8.0e-162
Identity = 299/496 (60.28%), Postives = 363/496 (73.19%), Query Frame = 0

Query: 16  RKPPTVAGGWPLIGHLPLFGG-SEPPHIKLSDLADKYGSIFSIRLGIHSAVVINNWEGAK 75
           RKPP  AGGWPLIGHL L GG S+ PHI L  LADKYGS+FSIR+G+HSA+VI++WE AK
Sbjct: 35  RKPPEAAGGWPLIGHLHLLGGKSQLPHIVLGSLADKYGSVFSIRIGVHSALVISSWELAK 94

Query: 76  ECFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLKLLRH 135
           ECFTT+D A+ SRPKL   K +GY+Y M  FSPY SYWR+MRKI +LELLSNRRL+LL+ 
Sbjct: 95  ECFTTNDVALASRPKLAGAKYLGYDYVMIGFSPYGSYWREMRKIISLELLSNRRLELLKD 154

Query: 136 VRETE-----------------------GGL-VDMKRWFRDLIMNLVLLVVAGKRYFGVA 195
           VR +E                       GG+ V+MK+WF DL +N+VL +VAGKRYFG A
Sbjct: 155 VRASEMETSLKELYKVWTEKKDGSDGGGGGVSVEMKQWFGDLALNVVLRMVAGKRYFGAA 214

Query: 196 TTV--EARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQW 255
            T   EARR Q A  E   L+G + ++GDAIP L W+D+GGHVKAMK T+K +D M T+W
Sbjct: 215 ATADKEARRCQKAFREFFHLMGQF-LVGDAIPYLGWMDLGGHVKAMKKTAKEMDCMATEW 274

Query: 256 LEEHKKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTCLTMVLGGSETT 315
           LEEH+K+R S E +K+  DFMD+MLSVL   GAD    FD DTV KS CL M+ GG++TT
Sbjct: 275 LEEHRKNRVSGE-SKQQQDFMDIMLSVLE--GADLG-DFDADTVNKSACLNMISGGTDTT 334

Query: 316 VVAVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGAG 375
           +V++ W LSLLLNNRHAL K QEELD H+GR   + E DI KL YL A+VKEA+RLY  G
Sbjct: 335 MVSLTWTLSLLLNNRHALIKAQEELDMHVGRGRLVNEGDISKLFYLQAIVKEALRLYPPG 394

Query: 376 SLIS-REVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHKDFDVK 435
            L   RE AED TI GFH+ KGT +  NLWKI  D ++W+EPL FKPERFLTTHKD DV+
Sbjct: 395 PLSGPREAAEDCTIGGFHVAKGTRVVFNLWKIQTDPKIWSEPLEFKPERFLTTHKDVDVR 454

Query: 436 GHQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGMTNMKA 484
           G  FELIPFG GRR CPG+SFGLQ++ L L  LLHAF+I TP +A VDM+ T G++N+K 
Sbjct: 455 GQNFELIPFGSGRRACPGISFGLQMLHLTLASLLHAFEISTPSNATVDMSATFGLSNIKT 514

BLAST of Tan0012704 vs. NCBI nr
Match: XP_040998670.1 (cytochrome P450 CYP82D47-like [Juglans microcarpa x Juglans regia])

HSP 1 Score: 578.9 bits (1491), Expect = 4.0e-161
Identity = 298/497 (59.96%), Postives = 365/497 (73.44%), Query Frame = 0

Query: 13  KNDRKPPTVAGGWPLIGHLPLFGG-SEPPHIKLSDLADKYGSIFSIRLGIHSAVVINNWE 72
           K  RKPP  AGGWPLIGHL L GG S+ PHI L  LA+KYGS+FSIR+G+HSAVVI++WE
Sbjct: 33  KISRKPPEAAGGWPLIGHLHLLGGKSQLPHIVLGSLAEKYGSVFSIRIGVHSAVVISSWE 92

Query: 73  GAKECFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLKL 132
            AKECFTT+D A+ SRPKL   K +GY+  MF FSPY SYWR+MRKI +LELLSNRRL+L
Sbjct: 93  MAKECFTTNDVALASRPKLAGAKHLGYDSVMFGFSPYGSYWREMRKIISLELLSNRRLEL 152

Query: 133 LRHVRETE---------------------GGL-VDMKRWFRDLIMNLVLLVVAGKRYFGV 192
           L+ VR +E                     GG+ V+MK+WF DL +N+VL +VAGKRYFG 
Sbjct: 153 LKDVRASEMETSLKELYKVWTEKKDGSDGGGVSVEMKQWFGDLALNVVLRMVAGKRYFGA 212

Query: 193 ATTV--EARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQ 252
           A     EA+R Q A  E   L+G + ++GDAIP L W+D+GGHVKAMK T+K +D M T+
Sbjct: 213 AAAADNEAQRCQKAFREFFHLMGQF-LVGDAIPYLGWMDLGGHVKAMKKTAKEMDCMATE 272

Query: 253 WLEEHKKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTCLTMVLGGSET 312
           WLEEH+K+RAS E +K+  DF+D+MLSVL   GAD    FD DTV KSTCL M+ GG++T
Sbjct: 273 WLEEHRKNRASGE-SKEQLDFIDIMLSVLE--GADLG-DFDADTVNKSTCLNMISGGTDT 332

Query: 313 TVVAVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGA 372
           T+V++ WALSLLLNNRHAL K QEELD H+GR   + E DI  L YL A+VKEA+RLY  
Sbjct: 333 TMVSLTWALSLLLNNRHALIKAQEELDMHVGRGRLVNEGDISNLFYLQAIVKEALRLYPP 392

Query: 373 GSLIS-REVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHKDFDV 432
           G L   RE  ED TI GFH+ KGT + +NLWKI  D ++W+EPL FKPERFLTTHKD DV
Sbjct: 393 GPLSGPREATEDCTIGGFHVAKGTRILVNLWKIQTDPKIWSEPLEFKPERFLTTHKDVDV 452

Query: 433 KGHQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGMTNMK 484
           KG  FELIPFG GRR CPG++FGLQ++ L L  L+HAF I TP +A VDM+ T G+TN+K
Sbjct: 453 KGQNFELIPFGSGRRACPGIAFGLQMLHLPLASLIHAFGISTPSNATVDMSATFGLTNIK 512

BLAST of Tan0012704 vs. NCBI nr
Match: KAG6672267.1 (hypothetical protein I3842_16G049500 [Carya illinoinensis])

HSP 1 Score: 578.2 bits (1489), Expect = 6.8e-161
Identity = 297/496 (59.88%), Postives = 362/496 (72.98%), Query Frame = 0

Query: 16  RKPPTVAGGWPLIGHLPLFGG-SEPPHIKLSDLADKYGSIFSIRLGIHSAVVINNWEGAK 75
           RKPP  AGGWPLIGHL L GG S+ PHI L  LADKYGS+FSIR+G+HSA+VI++WE AK
Sbjct: 35  RKPPEAAGGWPLIGHLHLLGGKSQLPHIVLGSLADKYGSVFSIRIGVHSALVISSWELAK 94

Query: 76  ECFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLKLLRH 135
           ECFTT+D A+ SRPKL   K +GY+Y M  FSPY SYWR+MRKI +LELLSNRR++LL+ 
Sbjct: 95  ECFTTNDVALASRPKLAGAKYLGYDYVMIGFSPYGSYWREMRKIISLELLSNRRIELLKD 154

Query: 136 VRETE-----------------------GGL-VDMKRWFRDLIMNLVLLVVAGKRYFGVA 195
           VR +E                       GG+ V+MK+WF DL +N+VL +VAGKRY G A
Sbjct: 155 VRASEMETSLKELYKVWTEKKDGSDGGGGGVSVEMKQWFGDLALNVVLRMVAGKRYVGAA 214

Query: 196 TTV--EARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQW 255
            T   E RR Q A  E   L+G + ++GDAIP L W+D+GGHVKAMK T+K +D M T+W
Sbjct: 215 ATADKEGRRCQKAFREFFHLMGQF-LVGDAIPYLGWMDLGGHVKAMKKTAKEMDCMATEW 274

Query: 256 LEEHKKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTCLTMVLGGSETT 315
           LEEH+K+R S E +K+  DFMD+MLSVL   GAD    FD DTV KSTCL M+ GG++TT
Sbjct: 275 LEEHRKNRVSGE-SKQQQDFMDIMLSVLE--GADLG-DFDADTVNKSTCLNMISGGTDTT 334

Query: 316 VVAVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGAG 375
           +V++ W LSLLLNNRHAL K QEELD H+GR   + E DI KL YL A+VKEA+RLY  G
Sbjct: 335 MVSLTWTLSLLLNNRHALIKAQEELDMHVGRGRLVNEGDISKLFYLQAIVKEALRLYPPG 394

Query: 376 SLIS-REVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHKDFDVK 435
            L   RE AED TI GFH+ KGT +  NLWKI  D ++W+EPL FKPERFLTTHKD DV+
Sbjct: 395 PLSGPREAAEDCTIGGFHVAKGTRVVFNLWKIQTDPKIWSEPLEFKPERFLTTHKDVDVR 454

Query: 436 GHQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGMTNMKA 484
           G  FELIPFG GRR CPG+SFGLQ++ L L  LLHAF+I TP +A VDM+ T G++N+K 
Sbjct: 455 GQNFELIPFGSGRRACPGISFGLQMLHLTLASLLHAFEISTPSNATVDMSATFGLSNIKT 514

BLAST of Tan0012704 vs. ExPASy TrEMBL
Match: A0A6J1BY79 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005812 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 2.9e-173
Identity = 312/458 (68.12%), Postives = 357/458 (77.95%), Query Frame = 0

Query: 17  KPPTVAGGWPLIGHLPLFGGSEPPHIKLSDLADKYGSIFSIRLGIHSAVVINNWEGAKEC 76
           KPP+V GGWPLIGHLPL GG + PH+KL  LAD+YG IFSI+LGIH AVVIN+WE AK+C
Sbjct: 43  KPPSVPGGWPLIGHLPLLGGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQC 102

Query: 77  FTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLKLLRHVR 136
           FTTHD AV  RPK+L+ +++GYNY++F F PY+SYWRKMRK TTL LLS+RRL+LLR VR
Sbjct: 103 FTTHDVAVSFRPKILAAQILGYNYAVFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVR 162

Query: 137 ETE--------------------GGLVDMKRWFRDLIMNLVLLVVAGKRYFGVATTV--- 196
           E+E                     GLV+MK+W  DL MNL+L VVAGKRYFG    V   
Sbjct: 163 ESEVNQAIKELYQIWKERRDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEA 222

Query: 197 EARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQWLEEHK 256
           EAR  Q A++E LRL+G++ +LGDA+P L WLDVGGHVKAMK TSK LD +LTQWLEEHK
Sbjct: 223 EARACQKAMKEFLRLMGLF-ILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHK 282

Query: 257 KDRASAEIAKKDTDFMDVMLSVLHET-----GADAAHGFDHDTVIKSTCLTMVLGGSETT 316
           K RAS + +K+D DFMDVMLS L ET      +D+ H FD DT+IK+TCLTMVLGGS+TT
Sbjct: 283 KKRASGK-SKEDKDFMDVMLSTLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTT 342

Query: 317 VVAVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGAG 376
            V V WALSLLLNN  AL KVQEE+D HIGRD +LKESD+ +L YL AVVKE MRLYGAG
Sbjct: 343 TVVVTWALSLLLNNHPALIKVQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAG 402

Query: 377 SLISREVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHKDFDVKG 436
            LI RE +ED  IDGFHI+KGT LFIN WKIHRD RVW EPL FKPERFLTTH+DFDV G
Sbjct: 403 GLILRETSEDFAIDGFHIEKGTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNG 462

Query: 437 HQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDI 447
            QFELIPFGGGRR CPGLSFGLQ+VQLILG LLHAFDI
Sbjct: 463 QQFELIPFGGGRRACPGLSFGLQMVQLILGALLHAFDI 498

BLAST of Tan0012704 vs. ExPASy TrEMBL
Match: A0A2I4EQH4 (cytochrome P450 CYP82D47-like OS=Juglans regia OX=51240 GN=LOC108991732 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 2.8e-160
Identity = 295/496 (59.48%), Postives = 364/496 (73.39%), Query Frame = 0

Query: 16  RKPPTVAGGWPLIGHLPLFGG-SEPPHIKLSDLADKYGSIFSIRLGIHSAVVINNWEGAK 75
           RKPP  AGGWPLIGHL L GG S+ PHI L  LA+KYGS+FSIR+G+HSAVVI++WE AK
Sbjct: 36  RKPPEAAGGWPLIGHLHLLGGKSQLPHIVLGSLAEKYGSVFSIRIGMHSAVVISSWEMAK 95

Query: 76  ECFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLKLLRH 135
           ECFTT+D A+ S PKL   K +GY+Y MF FSPY SYWR++RKI +LELLSNRRL+LL+ 
Sbjct: 96  ECFTTNDVALASHPKLAGAKHLGYDYVMFGFSPYGSYWREIRKIISLELLSNRRLELLKD 155

Query: 136 VRETE-----------------------GGL-VDMKRWFRDLIMNLVLLVVAGKRYFGVA 195
           VR +E                       GG+ V+MK+WF DL +N+VL +VAGKRYFG A
Sbjct: 156 VRASEMETSLKELYKVWTEKKDVSDGGGGGVSVEMKQWFGDLTLNVVLRMVAGKRYFGAA 215

Query: 196 TTV--EARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQW 255
                EA+R Q A  E   L+G + ++GDAIP L W+D+GGHVKAMK T+K +D + T+W
Sbjct: 216 AAADKEAQRCQKAFREFFHLMGQF-LVGDAIPYLGWMDLGGHVKAMKKTAKEMDCLATEW 275

Query: 256 LEEHKKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTCLTMVLGGSETT 315
           LEEH+K+R S E +K+  DF+D+MLSVL   GAD    FD DTV KSTCL M+ GG++TT
Sbjct: 276 LEEHRKNRVSGE-SKEQLDFIDIMLSVLE--GADLG-DFDADTVNKSTCLNMISGGTDTT 335

Query: 316 VVAVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGAG 375
           +V++ WALSLLLNNRHAL K QEELD H+GR   + E DI  L YL A+VKEA+RLY  G
Sbjct: 336 MVSLTWALSLLLNNRHALIKAQEELDMHVGRGRLVNEGDISNLFYLQAIVKEALRLYPPG 395

Query: 376 SLIS-REVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHKDFDVK 435
            L   RE  ED TI GFH+ KGT + +NLWKI  D ++W+EPL FKPERFLTTHKD DVK
Sbjct: 396 PLSGPREATEDCTIGGFHVAKGTRVLVNLWKIQTDPKIWSEPLEFKPERFLTTHKDVDVK 455

Query: 436 GHQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGMTNMKA 484
           G  FELIPFG GRR CPG++FGLQ++ L L  LLHAF+I TP +A VDM+ T G+TN+K+
Sbjct: 456 GQNFELIPFGSGRRACPGIAFGLQMLHLPLASLLHAFEISTPSNATVDMSATFGLTNIKS 515

BLAST of Tan0012704 vs. ExPASy TrEMBL
Match: A0A6J1BSL4 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005334 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 8.1e-160
Identity = 292/428 (68.22%), Postives = 328/428 (76.64%), Query Frame = 0

Query: 17  KPPTVAGGWPLIGHLPLFGGSEPPHIKLSDLADKYGSIFSIRLGIHSAVVINNWEGAKEC 76
           KPPTVAGGWPLIGHLPL  G E PHIKLS LA++YG IFSI+LGI  AVVIN+WE AK+C
Sbjct: 40  KPPTVAGGWPLIGHLPLLAGPELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQC 99

Query: 77  FTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLKLLRHVR 136
           FTTHD A+ SRP +L+ K++GYNY+MF F PY+SYWRKMRK T L LLSNRRL+LLR+VR
Sbjct: 100 FTTHDVAISSRPNILASKILGYNYAMFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVR 159

Query: 137 ETEGG--------------------LVDMKRWFRDLIMNLVLLVVAGKRYFGVATTV--- 196
           E+E                      LV+MK+W  DL MNL+L VVAGKRYFG A  V   
Sbjct: 160 ESEVNQAIKELYTAWKERRDEQGQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEA 219

Query: 197 EARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQWLEEHK 256
           EAR    A +E LRL+G++  LGD +P L WLDVGGHVKAMK TSK LD MLT+WLEEH+
Sbjct: 220 EARACHRATKEFLRLMGLF-TLGDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHR 279

Query: 257 KDRASAEIAKKDTDFMDVMLSVLHETGA----DAAHGFDHDTVIKSTCLTMVLGGSETTV 316
           KDRAS   +K+D DFMDVMLSVL E  A    D+AH FD DT+IK+TCLTMVLGGS+TTV
Sbjct: 280 KDRASGR-SKEDKDFMDVMLSVLEEETANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTV 339

Query: 317 VAVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGAGS 376
           V V WALSLLLNNR ALRKVQ+ELD  IGRD +L ESDI KL Y  AVVKE MRLYG   
Sbjct: 340 VVVTWALSLLLNNRPALRKVQQELDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVG 399

Query: 377 LISREVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHKDFDVKGH 418
           L  RE +ED  IDGFHI+KGTWLF+NLWKIHRDERVW EPL FKPERFLTTHKDFDVKG 
Sbjct: 400 LFVRETSEDFAIDGFHIEKGTWLFMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQ 459

BLAST of Tan0012704 vs. ExPASy TrEMBL
Match: A0A6J5TPS2 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS7991 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 3.1e-159
Identity = 289/508 (56.89%), Postives = 362/508 (71.26%), Query Frame = 0

Query: 1   MKRVANGGEVKGKNDRKPPTVAGGWPLIGHLPLFGGSEPPHIKLSDLADKYGSIFSIRLG 60
           +KR ++  + KG    K P VAGGWPL+GH+ LFGGS+ PHI L+ L DKYG  F+I +G
Sbjct: 26  IKRSSSAAKAKGP---KLPKVAGGWPLLGHVGLFGGSQLPHITLASLVDKYGPAFTINIG 85

Query: 61  IHSAVVINNWEGAKECFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITT 120
           IHSA+VI+ WE AK+CFTT+D  V SRP  L+ K +GYN++MF FSPY  YWR+MRK+T+
Sbjct: 86  IHSALVISTWEVAKDCFTTNDIVVSSRPPTLAAKHLGYNFAMFGFSPYGPYWREMRKLTS 145

Query: 121 LELLSNRRLKLLRHVRETE--------------------GGLVDMKRWFRDLIMNLVLLV 180
           LELLSNRRL+LL+ VR +E                      LV+MK+WF DL +N++L +
Sbjct: 146 LELLSNRRLELLKKVRVSEVEMSLKELYTLWIKRKESSGERLVEMKQWFGDLTLNVILRM 205

Query: 181 VAGKRYF---GVATTVEARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTT 240
           VAGKR F    ++   EARRWQ A+ E  RL G++ VLGDA+P L W D+GG  KAMK T
Sbjct: 206 VAGKRCFMNGNLSEEKEARRWQKAMREFFRLAGLF-VLGDAVPWLSWFDLGGQQKAMKKT 265

Query: 241 SKVLDDMLTQWLEEHKKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTC 300
           +K LD ++ +WLEEHK+         KD DFMDVMLS ++ T  D A GFD DTVIK+TC
Sbjct: 266 AKELDSIVAEWLEEHKQKITEG----KDQDFMDVMLSTINGTVTDVA-GFDADTVIKATC 325

Query: 301 LTMVLGGSETTVVAVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAV 360
           L ++ GGS+TT+V + WALSLLLNNR  L+KV EELD ++G+   L+ESDI  L YL A 
Sbjct: 326 LILIAGGSDTTMVTLTWALSLLLNNRQVLKKVYEELDQYVGKGRLLEESDINNLVYLQAT 385

Query: 361 VKEAMRLYGAGSLI-SREVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPER 420
           VKEAMRL  AG L   RE  ED T+ G+H+ KGTWL +NLWKI  D RVWA+P+ FKPER
Sbjct: 386 VKEAMRLCPAGPLSGQREFTEDCTVGGYHVPKGTWLLVNLWKIQTDPRVWADPMEFKPER 445

Query: 421 FLTTHKDFDVKGHQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDM 480
           FLTTHKD DV+G QFEL+PFG GRR CPG+SFGLQ+  + L   LH FD+ T E+A VDM
Sbjct: 446 FLTTHKDVDVRGQQFELMPFGSGRRACPGISFGLQMTLITLASFLHWFDVTTQENAPVDM 505

Query: 481 TGTIGMTNMKATPLEVLIKPRLSPMLYD 485
           TG+IG+TN+K TPL VL+KPRLSP LY+
Sbjct: 506 TGSIGLTNIKLTPLNVLVKPRLSPDLYE 524

BLAST of Tan0012704 vs. ExPASy TrEMBL
Match: M5XNX5 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa023495mg PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 2.0e-158
Identity = 289/508 (56.89%), Postives = 358/508 (70.47%), Query Frame = 0

Query: 1   MKRVANGGEVKGKNDRKPPTVAGGWPLIGHLPLFGGSEPPHIKLSDLADKYGSIFSIRLG 60
           +KR ++  + KG    K P VAGGWPL+GH  LF GS+ PHI L+ L DKYG  F+I +G
Sbjct: 26  IKRSSSAAKAKGP---KLPKVAGGWPLLGHFGLFRGSQLPHIALASLVDKYGPAFTINIG 85

Query: 61  IHSAVVINNWEGAKECFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITT 120
           IHSA+VI+ WE AK+CFTT+D  V SRP  L  K +GYN++MF FSPY SYWR+MRK+T+
Sbjct: 86  IHSALVISTWEAAKDCFTTNDIVVSSRPATLGAKHLGYNFAMFGFSPYGSYWREMRKLTS 145

Query: 121 LELLSNRRLKLLRHVRETE--------------------GGLVDMKRWFRDLIMNLVLLV 180
           LELLSNRRL+LL+ VR +E                      LV+MK+WF DL +N++  +
Sbjct: 146 LELLSNRRLELLKKVRVSEVEMSLKELYTLWIKRKESSGERLVEMKQWFGDLTLNVIFRM 205

Query: 181 VAGKRYF---GVATTVEARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTT 240
           VAGKR F    ++   EARRWQ A+ E   L G++ VLGDA+P L WLD+GG  KAMK T
Sbjct: 206 VAGKRCFMNGNLSEEKEARRWQKAMREFFHLAGLF-VLGDAVPWLSWLDLGGQQKAMKKT 265

Query: 241 SKVLDDMLTQWLEEHKKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTC 300
           +K LD ++ +WLEEHK+ R       KD DFMDVMLS +   G D A GFD DTVIK+TC
Sbjct: 266 AKELDSIVAEWLEEHKQKRTKG----KDQDFMDVMLSAI--DGTDVA-GFDADTVIKATC 325

Query: 301 LTMVLGGSETTVVAVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAV 360
           L ++ G S+TT+V + W LSLLLNNR  L+KV EELD ++G+   L ESDI  L YL A 
Sbjct: 326 LILIAGASDTTMVTLTWTLSLLLNNRQVLKKVYEELDQYVGKGRLLDESDINNLVYLQAT 385

Query: 361 VKEAMRLYGAGSLI-SREVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPER 420
           VKEAMRL  AG L   RE  ED T+ G+H+ KGTWL +NLWKI  D RVWA+P+ FKPER
Sbjct: 386 VKEAMRLCPAGPLSGQREFTEDCTVGGYHVPKGTWLLVNLWKIQTDPRVWADPMEFKPER 445

Query: 421 FLTTHKDFDVKGHQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDM 480
           FLTTHKD DV+G QFEL+PFG GRR CPG+SFGLQ+  L L   LH+FD+ T E+A VDM
Sbjct: 446 FLTTHKDVDVRGQQFELMPFGSGRRACPGISFGLQMTLLTLASFLHSFDVTTQENAPVDM 505

Query: 481 TGTIGMTNMKATPLEVLIKPRLSPMLYD 485
           TG+IG+TN+K TPL+VL+KPRLSP LYD
Sbjct: 506 TGSIGLTNIKLTPLDVLVKPRLSPNLYD 522

BLAST of Tan0012704 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 449.1 bits (1154), Expect = 4.4e-126
Identity = 231/495 (46.67%), Postives = 328/495 (66.26%), Query Frame = 0

Query: 17  KPPTVAGGWPLIGHLPLFGGSEP-PHIKLSDLADKYGSIFSIRLGIHSAVVINNWEGAKE 76
           K P  +G WP+IGHL L GG E   +  L  +AD YG   S++LG + A V++++E AK+
Sbjct: 31  KAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKD 90

Query: 77  CFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLKLLRHV 136
           CFT +D A+ SRP   + K MGYN+++F F+PY+++WR+MRKI T+ELLSNRRL++L+HV
Sbjct: 91  CFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHV 150

Query: 137 RETE---------------GG----LVDMKRWFRDLIMNLVLLVVAGKRYFGVATTV--- 196
           R +E               GG    +VD+K W  D+ +N+++ +VAGKRYFG   +V   
Sbjct: 151 RVSEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLNMIVRMVAGKRYFGGGGSVSSE 210

Query: 197 ---EARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQWLE 256
              EA + + AI +   L+GI+ V  DA P L + D+ GH K MK T   LD +L +W+E
Sbjct: 211 DTEEAMQCKKAIAKFFHLIGIFTV-SDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIE 270

Query: 257 EHKKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTCLTMVLGGSETTVV 316
            H++ R  +   + D+DF+DVM+S L E G  +   +D +T IKSTCL ++LGGS+T+  
Sbjct: 271 NHRQQRKFSGTKENDSDFIDVMMS-LAEQGKLSHLQYDANTSIKSTCLALILGGSDTSAS 330

Query: 317 AVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGAGSL 376
            + WA+SLLLNN+  L+K Q+E+D H+GRD  +++SDIE L YL A++KE +RLY AG L
Sbjct: 331 TLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYLQAIIKETLRLYPAGPL 390

Query: 377 IS-REVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTH-KDFDVKG 436
           +  RE  ED T+ G+++  GT L +N+WKI RD +V+ EP  F+PERF+T   K+FDV+G
Sbjct: 391 LGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEAKEFDVRG 450

Query: 437 HQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGMTNMKAT 484
             FEL+PFG GRR CPG S  +Q++ L L R LH+FD+ T  D  VDM+   G+T  KAT
Sbjct: 451 QNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTVMDMPVDMSENPGLTIPKAT 510

BLAST of Tan0012704 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 442.6 bits (1137), Expect = 4.1e-124
Identity = 231/494 (46.76%), Postives = 322/494 (65.18%), Query Frame = 0

Query: 17  KPPTVAGGWPLIGHLPLFGGSEP-PHIKLSDLADKYGSIFSIRLGIHSAVVINNWEGAKE 76
           K P  +G WP+IGHL L  G E   +  L  +AD+YG   S+RLG     V++++E AK+
Sbjct: 31  KAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPAMSLRLGSSETFVVSSFEVAKD 90

Query: 77  CFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLKLLRHV 136
           CFT +D A+ SRP   + K MGY+ ++F F+PY+++WR+MRKI TLELLSNRRL++L+HV
Sbjct: 91  CFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWREMRKIATLELLSNRRLQMLKHV 150

Query: 137 RETE---------------GG----LVDMKRWFRDLIMNLVLLVVAGKRYFGVAT----- 196
           R +E               GG    +VD+K W  D+ +N+++ +VAGKRYFG  +     
Sbjct: 151 RVSEISMVMQDLYSLWVKKGGSEPVMVDLKSWLEDMSLNMMVRMVAGKRYFGGGSLSPED 210

Query: 197 TVEARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQWLEE 256
             EAR+ +  +     L+GI+ V  DA P L W D  GH K MK T + LD +L +W+E 
Sbjct: 211 AEEARQCRKGVANFFHLVGIFTV-SDAFPKLGWFDFQGHEKEMKQTGRELDVILERWIEN 270

Query: 257 HKKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTCLTMVLGGSETTVVA 316
           H++ R  +     D+DF+DVMLS L E G  +    D  T IKSTCL ++LGGSET+   
Sbjct: 271 HRQQRKVSGTKHNDSDFVDVMLS-LAEQGKFSHLQHDAITSIKSTCLALILGGSETSPST 330

Query: 317 VIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGAGSLI 376
           + WA+SLLLNN+  L+K Q+E+D H+GRD  +++SDIE L Y+ A++KE +RLY AG L+
Sbjct: 331 LTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVYIQAIIKETLRLYPAGPLL 390

Query: 377 S-REVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTH-KDFDVKGH 436
             RE  ED T+ G+++++GT + +N+WKI RD RV+ EP  F+PERF+T   K+FDV+G 
Sbjct: 391 GHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFRPERFITGEAKEFDVRGQ 450

Query: 437 QFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGMTNMKATP 484
            FEL+PFG GRR CPG S  +Q++ L L R L +FD+ T  D  VDMT + G+T  KATP
Sbjct: 451 NFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMDMPVDMTESPGLTIPKATP 510

BLAST of Tan0012704 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 411.8 bits (1057), Expect = 7.8e-115
Identity = 226/495 (45.66%), Postives = 311/495 (62.83%), Query Frame = 0

Query: 17  KPPTVAGGWPLIGHLPLFGGSEP-PHIKLSDLADKYGSIFSIRLGIHSAVVINNWEGAKE 76
           K P  +G WP+IGHL L GG E   +  L  +AD YG   S+RLG     V +++E AK+
Sbjct: 31  KAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLRLGSSETFVGSSFEVAKD 90

Query: 77  CFTTHDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLKLLRHV 136
           CFT +D A+ S     + K MGY            +W +MRKI  +ELLSNRRL++L +V
Sbjct: 91  CFTVNDKALASL-MTAAAKHMGY-----------VFWLEMRKIAMIELLSNRRLQMLNNV 150

Query: 137 RETE---------------GG----LVDMKRWFRDLIMNLVLLVVAGKRYFG------VA 196
           R +E               GG    +VD+K W  D+I N+++ +VAGKRYFG        
Sbjct: 151 RVSEISMGVKDLYSLWVKKGGSEPVMVDLKSWLEDMIANMIMRMVAGKRYFGGGGAESSE 210

Query: 197 TTVEARRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQWLE 256
            T EAR+W+  I +   L+GI+ V  DA P L WLD+ GH K MK T + LD +L +W+E
Sbjct: 211 HTEEARQWRKGIAKFFHLVGIFTV-SDAFPKLGWLDLQGHEKEMKQTRRELDVILERWIE 270

Query: 257 EHKKDRASAEIAKKDTDFMDVMLSVLHETGADAAHGFDHDTVIKSTCLTMVLGGSETTVV 316
            H++ R  +     D+DF+DVMLS L E G  +   +D +T IK+TCL ++LGGSET+  
Sbjct: 271 NHRQQRKVSGTKHNDSDFVDVMLS-LAEQGKLSHLQYDANTCIKTTCLALILGGSETSPS 330

Query: 317 AVIWALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGAGSL 376
            + WA+SLLLNN+  L+KVQ+E+D H+GRD  +++SDI+ L YL A++KE +RLY A  L
Sbjct: 331 TLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIKNLVYLQAIIKETLRLYPAAPL 390

Query: 377 IS-REVAEDLTIDGFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTH-KDFDVKG 436
           +  RE  ED T+ G+++  GT L +N+WKI RD +V+ EP  F+PERF+T   KDFDV+G
Sbjct: 391 LGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEAKDFDVRG 450

Query: 437 HQFELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGMTNMKAT 484
             FEL+PFG GRR CPG S  +Q++ L L R LH+F++ T  D  VDM+ + G+T  KAT
Sbjct: 451 QNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVKTVLDRPVDMSESPGLTITKAT 510

BLAST of Tan0012704 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 379.4 bits (973), Expect = 4.3e-105
Identity = 213/493 (43.20%), Postives = 290/493 (58.82%), Query Frame = 0

Query: 19  PTVAGGWPLIGHLPLFGGSEPPHIKLSDLADKYGSIFSIRLGIHSAVVINNWEGAKECFT 78
           P V G WPL+GHL LF    P H+    +AD YG +F  +LG    ++IN+ E AKE +T
Sbjct: 35  PMVPGAWPLLGHLHLFDTVNPTHVTFGAMADVYGPVFMAKLGSIKVMIINSKEVAKEIYT 94

Query: 79  THDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNR--RLKLLRHVR 138
            HD  +  RP+L + KL+GYN S   FSPY  YWR++RKI   EL S     + ++   R
Sbjct: 95  VHD-KLLERPELTASKLLGYNDSFLTFSPYGLYWREIRKIAVSELFSTSGVDMHMVSRAR 154

Query: 139 ET------------------EGGLVDMKRWFRDLIMNLVLLVVAGKRYFGV---ATTVEA 198
           E                   EG LVDMK+ F DL  N+ L++V+GKRYFG        EA
Sbjct: 155 EADLAFRALYVRWEKRGKPKEGVLVDMKQEFIDLTANISLMMVSGKRYFGENPNCEVKEA 214

Query: 199 RRWQNAIEESLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQWLEEHKKD 258
           RR    I E L    ++ +L D  P L +LD     + MK T+K LD +   W+EEHK  
Sbjct: 215 RRCGKLIREFLDYFALF-LLSDVAPVLGFLD-WKTKRGMKRTAKGLDKVAEGWIEEHKNK 274

Query: 259 RASAEIAKKDTDFMDVMLSVLHETGADAAHGF-DHDTVIKSTCLTMVLGGSETTVVAVIW 318
           R  ++  + + D++D+++ +L   G D   G  D  T IK+ CL +VL GSET +V ++W
Sbjct: 275 R--SDHGRSENDYLDILIKIL---GQDKIPGLSDTHTKIKALCLNLVLAGSETAIVVLVW 334

Query: 319 ALSLLLNNRHALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGAGSLIS-R 378
           A+SLLLNN H LRK QEELD+ IG++  ++E DI+ L YL A+VKE  RLY    L++ R
Sbjct: 335 AVSLLLNNPHVLRKAQEELDSKIGKERVVEELDIKDLVYLQAIVKETFRLYPPVPLVAYR 394

Query: 379 EVAEDLTID--GFHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHKDFDVKGHQF 438
            V ED  I     H+  GT L ++ WKIHRD  VW+ P  F+PERFLT++++ DV G  +
Sbjct: 395 AVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNVWSNPEQFEPERFLTSNRELDVGGQSY 454

Query: 439 ELIPFGGGRRVCPGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLE 485
           +  PFG GRR CP +  G+++V  +L R LH+FD+  P    VDMT + G+ N KATPLE
Sbjct: 455 KFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFDLARPSSQDVDMTESNGLVNHKATPLE 514

BLAST of Tan0012704 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 341.3 bits (874), Expect = 1.3e-93
Identity = 185/480 (38.54%), Postives = 275/480 (57.29%), Query Frame = 0

Query: 19  PTVAGGWPLIGHLPLFGGSEPPHIKLSDLADKYGSIFSIRLGIHSAVVINNWEGAKECFT 78
           P   G  PL GHL L  G +    KL+ ++ K+G IFS++LG +  VV ++ +  K+CFT
Sbjct: 40  PEPLGALPLFGHLHLLRGKKLLCKKLAAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFT 99

Query: 79  THDAAVFSRPKLLSGKLMGYNYSMFIFSPYNSYWRKMRKITTLELLSNRRLKLLRHVRET 138
           T+D A  +RP +  G+ +GYN +    +PY  YWR++RKI T+ L SN  +++L H+R +
Sbjct: 100 TNDLATATRPNIAFGRYVGYNNASLTLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSS 159

Query: 139 E------------GG--LVDMKRWFRDLIMNLVLLVVAGKRYFGVATTVEARRWQNAIEE 198
           E            GG  +V +   F  L  N++L  + GKR        +  R++ A++ 
Sbjct: 160 EVNTLIKHLYKGNGGTSIVKIDMLFEFLTFNIILRKMVGKRIGFGEVNSDEWRYKEALKH 219

Query: 199 SLRLLGIYHVLGDAIPCLEWLDVGGHVKAMKTTSKVLDDMLTQWLEEHKKDRASAEIAKK 258
               L +  ++GD IP L WLD   +   MK   K LD + T+WL EH K R+  E  ++
Sbjct: 220 -CEYLAVIPMIGDVIPWLGWLDFAKN-SQMKRLFKELDSVNTKWLHEHLKKRSRNEKDQE 279

Query: 259 DTDFMDVMLSVLHETGADAAHGFDHDTVIKSTCLTMVLGGSETTVVAVIWALSLLLNNRH 318
            T  MD++L +L E    + H    D ++K+T L + L GS++T + + WA+SLLLNN  
Sbjct: 280 RT-IMDLLLDILPEDIVISGH--VRDVIVKATILALTLTGSDSTSITLTWAVSLLLNNPA 339

Query: 319 ALRKVQEELDTHIGRDTKLKESDIEKLTYLAAVVKEAMRLYGAGSLIS-REVAEDLTIDG 378
           AL   QEE+D  +G+   ++ESDI+ L YL A+VKE  RLY    L   RE  ED  + G
Sbjct: 340 ALEAAQEEIDNSVGKGRWIEESDIQNLKYLQAIVKETHRLYPPAPLTGIREAREDCFVGG 399

Query: 379 FHIKKGTWLFINLWKIHRDERVWAEPLVFKPERFLTTHKDFDVKGHQFELIPFGGGRRVC 438
           + ++KGT L +N+WK+HRD ++W +P  FKPERF+      +     FE IPFG GRR C
Sbjct: 400 YRVEKGTRLLVNIWKLHRDPKIWPDPKTFKPERFMEDKSQCEKS--NFEYIPFGSGRRSC 459

Query: 439 PGLSFGLQLVQLILGRLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLEVLIKPRLSPMLY 484
           PG++ GL++V  +L RLL  F++    D  +DM    G+   K  P+EV++ PRL P LY
Sbjct: 460 PGVNLGLRVVHFVLARLLQGFELHKVSDEPLDMAEGPGLALPKINPVEVVVMPRLDPKLY 512

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O498582.2e-13849.70Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1[more]
O498596.6e-13550.00Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1[more]
Q430683.1e-13247.40Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2[more]
A0A0N7F2973.8e-13048.58Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP... [more]
O819728.4e-13048.41Cytochrome P450 82A2 OS=Glycine max OX=3847 GN=CYP82A2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038883369.13.8e-22078.64cytochrome P450 CYP82D47-like [Benincasa hispida][more]
XP_022133138.15.9e-17368.12cytochrome P450 CYP82D47-like [Momordica charantia][more]
KAG2663852.18.0e-16260.28hypothetical protein I3760_16G051800 [Carya illinoinensis][more]
XP_040998670.14.0e-16159.96cytochrome P450 CYP82D47-like [Juglans microcarpa x Juglans regia][more]
KAG6672267.16.8e-16159.88hypothetical protein I3842_16G049500 [Carya illinoinensis][more]
Match NameE-valueIdentityDescription
A0A6J1BY792.9e-17368.12cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005812 PE=... [more]
A0A2I4EQH42.8e-16059.48cytochrome P450 CYP82D47-like OS=Juglans regia OX=51240 GN=LOC108991732 PE=3 SV=... [more]
A0A6J1BSL48.1e-16068.22cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005334 PE=... [more]
A0A6J5TPS23.1e-15956.89Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS7991 PE=3 SV... [more]
M5XNX52.0e-15856.89Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa023495mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G31940.14.4e-12646.67cytochrome P450, family 82, subfamily C, polypeptide 4 [more]
AT4G31970.14.1e-12446.76cytochrome P450, family 82, subfamily C, polypeptide 2 [more]
AT4G31950.17.8e-11545.66cytochrome P450, family 82, subfamily C, polypeptide 3 [more]
AT2G25160.14.3e-10543.20cytochrome P450, family 82, subfamily F, polypeptide 1 [more]
AT3G25180.11.3e-9338.54cytochrome P450, family 82, subfamily G, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 292..318
score: 35.48
coord: 413..423
score: 54.58
coord: 423..446
score: 28.98
coord: 375..399
score: 41.47
coord: 152..170
score: 17.55
coord: 272..289
score: 24.84
coord: 48..67
score: 36.51
IPR001128Cytochrome P450PRINTSPR00385P450coord: 336..347
score: 38.94
coord: 283..300
score: 34.12
coord: 414..423
score: 58.69
IPR001128Cytochrome P450PFAMPF00067p450coord: 23..458
e-value: 2.2E-82
score: 277.2
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 9..479
e-value: 4.2E-113
score: 380.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 23..477
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR47947CYTOCHROME P450 82C3-RELATEDcoord: 13..483
NoneNo IPR availablePANTHERPTHR47947:SF5CYTOCHROME P450 FAMILY PROTEINcoord: 13..483
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 416..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0012704.1Tan0012704.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098542 defense response to other organism
cellular_component GO:0016020 membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen