Tan0012661 (gene) Snake gourd v1

Overview


Name	Tan0012661
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	nifU-like protein 2, chloroplastic
Location	LG04: 202413 .. 204069 (+)
RNA-Seq Expression	Tan0012661
Synteny	Tan0012661

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCATGCCCTGTTGTTGAATTCCTACCCTTACACCGGAGCCCCCCTAACCCTTCAAACTCCTTCTTCTTCTCGTACCTTCAAGGTCTCTTTCATTTCTCGCTATTACTCTTTTTTTTTTTTTTAATTTTATTCCTCTCAACCAAATTAATATGTTTTATTTTGCAATTTCTTGTAGATCTCAAACTTCTTCTTCAGGAGGAGATTATCTCGTTCCCTCCGAATTCCATTGCCCAATTCACGCAGCCAAGGTACGATTTCCTTTTTTCCTTCTATTCGACTGCTTTAAGTTCTGGTTCTTGATCATCTCTTAAATTGTTCACTGCTTATGGCTGATATGCGATTAGTTCTCGATGTTAGAACCAAGAGAATGCATACTTACTCTTTCTTTTTAGGTTACCAAATCTTTGTCATTTGATTCGAAATTTGCTGCTTACAGTTATAAGAGCTGTGGCAACTTCCGATTCGGCACTGGAATTGCCACTGACTGCAGAAAATGTTGAAAGTGTGTTGGACGAGGTTCGACCATATCTTATTGCAGATGGTGGAAATGTGGCATTACATGAAATAGACGGAAATGTTGTACGATTGAAGCTACAAGGAGCATGCGGCTCATGTCCGAGTTCTGTTATGACCATGAAGATGGGAATTGAACGGCGTTTGATGGAGAAGATCCCTGAAATAGTTGCTGTGGAACCAATAGCAGATGAAGAAACTGGATTGGAGCTCAACGAGGACAATATAGAGAAGGTAAATAAATGTTCACTTTCTATCATGCGTATTCATTCTATGATTTAGATTAGATGTAAACTTATAGGTCGTTTCTGAATCCGAATTTTAGCGCCTTTATACACCCATGACCCACCCTCCTGCAATCTCTAACCTAGGGCTAGGGATACAACTTATACTGAAACAGGAGGGCTGCTCCCCTTCAAGGTAATTGGACTCTTTTTATGGAGGAAGAGGCACAGGGTAGAATTAAGGAAGCATTCTTTTTACTCAACATCGACAATACTTCATATGCCATCTTTCACTTTCTTGGATAGTCTTTAGTTTTTATAAAACAATGCTTCTAAGTTCTAGCTGTTTGGGAACCTATTATTTCTGTATGGTTACATCTTCCTTTCCAACTTCATAGCAATATCAATTAAATGCTTCCAATGTCCATGCTACTTGGTATTGGTCCAAAACTGCGACCAGCTTGATTAGGCCTGCAACATTTGTAGGCAATGAACCTGATATTAATGCAACATTAAAGACCTTTGGATTTTCTTGTCATGTATGATATGAACCAAGGGCTGTATAATTATTATCTATTCAACCTTGATGGAGACTCCAATGCCACTTTGAACTCCATGAAATATTATGATCAAATGTTATTATTGATAATTTTTTTCAGTGATGGCATAAGTTCGATTTAGACTATGGCATTTAGAAGCTGAATTTTCTTTATACCCTTTTCTTAGGTGCTTGAAGAGATAAGACCTTACCTTGTCGGGGCAGCGGGAGGATCTCTTGAACTTGTGGGTATCGAGGAGCCAATAGTTAAAGTTCGGATCACAGGGCCAGCTGCTGGGGTCATGACAGTTCGTGTGGCTGTTACACAGAAATTGCGAGAGAAGATACCAACCATAGCGGCAGTTCAACTTTTATCATAA

mRNA sequence

ATGCATGCCCTGTTGTTGAATTCCTACCCTTACACCGGAGCCCCCCTAACCCTTCAAACTCCTTCTTCTTCTCGTACCTTCAAGATCTCAAACTTCTTCTTCAGGAGGAGATTATCTCGTTCCCTCCGAATTCCATTGCCCAATTCACGCAGCCAAGTTATAAGAGCTGTGGCAACTTCCGATTCGGCACTGGAATTGCCACTGACTGCAGAAAATGTTGAAAGTGTGTTGGACGAGGTTCGACCATATCTTATTGCAGATGGTGGAAATGTGGCATTACATGAAATAGACGGAAATGTTGTACGATTGAAGCTACAAGGAGCATGCGGCTCATGTCCGAGTTCTGTTATGACCATGAAGATGGGAATTGAACGGCGTTTGATGGAGAAGATCCCTGAAATAGTTGCTGTGGAACCAATAGCAGATGAAGAAACTGGATTGGAGCTCAACGAGGACAATATAGAGAAGGTGCTTGAAGAGATAAGACCTTACCTTGTCGGGGCAGCGGGAGGATCTCTTGAACTTGTGGGTATCGAGGAGCCAATAGTTAAAGTTCGGATCACAGGGCCAGCTGCTGGGGTCATGACAGTTCGTGTGGCTGTTACACAGAAATTGCGAGAGAAGATACCAACCATAGCGGCAGTTCAACTTTTATCATAA

Coding sequence (CDS)

ATGCATGCCCTGTTGTTGAATTCCTACCCTTACACCGGAGCCCCCCTAACCCTTCAAACTCCTTCTTCTTCTCGTACCTTCAAGATCTCAAACTTCTTCTTCAGGAGGAGATTATCTCGTTCCCTCCGAATTCCATTGCCCAATTCACGCAGCCAAGTTATAAGAGCTGTGGCAACTTCCGATTCGGCACTGGAATTGCCACTGACTGCAGAAAATGTTGAAAGTGTGTTGGACGAGGTTCGACCATATCTTATTGCAGATGGTGGAAATGTGGCATTACATGAAATAGACGGAAATGTTGTACGATTGAAGCTACAAGGAGCATGCGGCTCATGTCCGAGTTCTGTTATGACCATGAAGATGGGAATTGAACGGCGTTTGATGGAGAAGATCCCTGAAATAGTTGCTGTGGAACCAATAGCAGATGAAGAAACTGGATTGGAGCTCAACGAGGACAATATAGAGAAGGTGCTTGAAGAGATAAGACCTTACCTTGTCGGGGCAGCGGGAGGATCTCTTGAACTTGTGGGTATCGAGGAGCCAATAGTTAAAGTTCGGATCACAGGGCCAGCTGCTGGGGTCATGACAGTTCGTGTGGCTGTTACACAGAAATTGCGAGAGAAGATACCAACCATAGCGGCAGTTCAACTTTTATCATAA

Protein sequence

MHALLLNSYPYTGAPLTLQTPSSSRTFKISNFFFRRRLSRSLRIPLPNSRSQVIRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIRPYLVGAAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPTIAAVQLLS

Homology

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BLAST of Tan0012661 vs. ExPASy Swiss-Prot
Match: Q93W20 (NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 7.8e-75
Identity = 148/184 (80.43%), Postives = 165/184 (89.67%), Query Frame = 0


Query: 35  RRRLSRSLRIPLPNSRSQVIRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALH 94
           RR LSR L       RS+V++AVAT D  LE+PLT ENVESVLDE+RPYL++DGGNVALH
Sbjct: 52  RRGLSRFLSSRQLFRRSKVVKAVATPDPILEVPLTEENVESVLDEIRPYLMSDGGNVALH 111

Query: 95  EIDGNVVRLKLQGACGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNI 154
           EIDGN+VR+KLQGACGSCPSS MTMKMGIERRLMEKIPEIVAVE + DEETGLELNE+NI
Sbjct: 112 EIDGNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEETGLELNEENI 171

Query: 155 EKVLEEIRPYLVGAAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPTIAA 214
           EKVLEEIRPYL+G A GSL+LV IE+PIVK+RITGPAAGVMTVRVAVTQKLREKIP+IAA
Sbjct: 172 EKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRITGPAAGVMTVRVAVTQKLREKIPSIAA 231

Query: 215 VQLL 219
           VQL+
Sbjct: 232 VQLI 235

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BLAST of Tan0012661 vs. ExPASy Swiss-Prot
Match: Q84LK7 (NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 5.8e-62
Identity = 136/214 (63.55%), Postives = 168/214 (78.50%), Query Frame = 0


Query: 14  APLTLQTPSSSRTFKISNFFF---RRRLSRSLR-IPLPNSRSQ--VIRAVATSD--SALE 73
           AP T  T SSS +FK++ + +   R   S + R +  PN +    V+ A+A  D  +A++
Sbjct: 15  APST--TTSSSASFKVAAYAWSSCRSSSSPATRLVAAPNHQRPPLVVGAIAGLDPVTAVQ 74

Query: 74  LPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSCPSSVMTMKMGIER 133
           LPLTA NVESVLD+VRPYL ADGG+VALHEI GNVVRLKLQGACGSCPSS++T+K GIER
Sbjct: 75  LPLTAGNVESVLDQVRPYLTADGGDVALHEIAGNVVRLKLQGACGSCPSSLITIKRGIER 134

Query: 134 RLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIRPYLVGAAGGSLELVGIEEPIVKV 193
           RLMEKIP++ AVEP+ D+ETGLELNE+N+EKVL EIRPYL G  GG L+ + I+ PIVKV
Sbjct: 135 RLMEKIPDVAAVEPVTDKETGLELNEENVEKVLNEIRPYLAGTGGGGLQFLMIKGPIVKV 194

Query: 194 RITGPAAGVMTVRVAVTQKLREKIPTIAAVQLLS 220
           R+TGPAA V TVR+AV++KLREKIP+I  VQLLS
Sbjct: 195 RLTGPAAVVRTVRIAVSKKLREKIPSIQIVQLLS 226

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BLAST of Tan0012661 vs. ExPASy Swiss-Prot
Match: Q84RQ7 (NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 1.4e-55
Identity = 116/154 (75.32%), Postives = 131/154 (85.06%), Query Frame = 0


Query: 66  LPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSCPSSVMTMKMGIER 125
           +PLT ENVE VLDEVRP L+ADGGNVALHEIDG VV LKLQGACGSCPSS MT+KMGIE 
Sbjct: 82  MPLTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIES 141

Query: 126 RLMEKIPEIVAVEPIADEET-GLELNEDNIEKVLEEIRPYLVGAAGGSLELVGIEEPIVK 185
           RL +KIPEI++VE   + ET GLELN++NIEKVL E+RPYL G  GG LELV I+  +VK
Sbjct: 142 RLRDKIPEIMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVK 201

Query: 186 VRITGPAAGVMTVRVAVTQKLREKIPTIAAVQLL 219
           VR+TGPAAGVMTVRVA+TQKLRE IP+I AVQLL
Sbjct: 202 VRLTGPAAGVMTVRVALTQKLRETIPSIGAVQLL 235

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BLAST of Tan0012661 vs. ExPASy Swiss-Prot
Match: Q93W77 (NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 5.5e-20
Identity = 59/147 (40.14%), Postives = 91/147 (61.90%), Query Frame = 0


Query: 62  SALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSCPSSVMTMKM 121
           SA    LT +NV+ VL++VRP+LI+DGGNV +  ++  VV LKLQGAC SCPSS  TM M
Sbjct: 79  SAQTFDLTPQNVDLVLEDVRPFLISDGGNVDVVSVEDGVVSLKLQGACTSCPSSSTTMTM 138

Query: 122 GIERRLMEKIPEIVA-VEPIADEETGLELNEDNIEKVLEEIRPYLVGAAGGSLELVGIEE 181
           GIER L EK  + +  +  + DEE   ++  + +   L+ +RP  +   GGS+E++ +E 
Sbjct: 139 GIERVLKEKFGDALKDIRQVFDEEV-KQITVEAVNAHLDILRP-AIKNYGGSVEVLSVEG 198

Query: 182 PIVKVRITGPAAGVMTVRVAVTQKLRE 208
               V+  GP +  M ++ A+ +K ++
Sbjct: 199 EDCVVKYVGPESIGMGIQAAIKEKFKD 223

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BLAST of Tan0012661 vs. ExPASy Swiss-Prot
Match: O32119 (Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224308 GN=yutI PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.8e-16
Identity = 37/70 (52.86%), Postives = 55/70 (78.57%), Query Frame = 0


Query: 71  ENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSCPSSVMTMKMGIERRLMEK 130
           E V+ VLD++RP+L+ DGG+  L ++D  +V+L+L GACGSCPSS +T+K GIER L+E+
Sbjct: 41  EQVQEVLDKLRPFLLRDGGDCELVDVDEGIVKLRLLGACGSCPSSTITLKAGIERALLEE 100

Query: 131 IPEIVAVEPI 141
           +P +V VE +
Sbjct: 101 VPGVVEVEQV 110

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BLAST of Tan0012661 vs. NCBI nr
Match: XP_022151795.1 (nifU-like protein 2, chloroplastic [Momordica charantia])

HSP 1 Score: 352.8 bits (904), Expect = 2.1e-93
Identity = 197/232 (84.91%), Postives = 203/232 (87.50%), Query Frame = 0


Query: 1   MHALLLNSYPYTGAPLTLQTPSSSRTFKISNFFFR------------RRLSRSLRIPLP- 60
           MHA++LNS PYT AP  L+TPSSSR FKIS  F              R+  RS RI LP 
Sbjct: 1   MHAVVLNSSPYTRAPQPLETPSSSRAFKISRPFLSHVSPSRERTPLWRKSCRSFRIRLPT 60

Query: 61  NSRSQVIRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQG 120
           NSR  V+RAVATS+SALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQG
Sbjct: 61  NSRRPVMRAVATSNSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQG 120

Query: 121 ACGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIRPYLVG 180
           ACGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIRPYLVG
Sbjct: 121 ACGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIRPYLVG 180

Query: 181 AAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPTIAAVQLLS 220
           AAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIP IAAVQLLS
Sbjct: 181 AAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPAIAAVQLLS 232

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BLAST of Tan0012661 vs. NCBI nr
Match: XP_008445979.1 (PREDICTED: nifU-like protein 2, chloroplastic [Cucumis melo])

HSP 1 Score: 348.6 bits (893), Expect = 3.9e-92
Identity = 192/227 (84.58%), Postives = 206/227 (90.75%), Query Frame = 0


Query: 1   MHALL--LNSYPYTGAPLTLQTPSSSRTFKISNFFFR-----RRLSRSLRIPLP-NSRSQ 60
           MHA++  LN+ PYT AP TL++PS SR FK+S FFFR     R+  RSLRI LP +SR++
Sbjct: 1   MHAVVWNLNTSPYTRAPQTLESPSCSRNFKVSRFFFRHVSPWRKSCRSLRIRLPSSSRTR 60

Query: 61  VIRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSC 120
           +IRAVAT +SALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGN+VRLKLQGACGSC
Sbjct: 61  LIRAVATPNSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNIVRLKLQGACGSC 120

Query: 121 PSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIRPYLVGAAGGS 180
           PSSV TMKMGIERRLMEKIPEIVAVEPIAD ETGLELNE+NIEKVLEEIRPYLVGAAGGS
Sbjct: 121 PSSVTTMKMGIERRLMEKIPEIVAVEPIADVETGLELNEENIEKVLEEIRPYLVGAAGGS 180

Query: 181 LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPTIAAVQLLS 220
           LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIP IAAVQLLS
Sbjct: 181 LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPAIAAVQLLS 227

[Close]

BLAST of Tan0012661 vs. NCBI nr
Match: KAA0034154.1 (nifU-like protein 2 [Cucumis melo var. makuwa] >TYK15765.1 nifU-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 348.6 bits (893), Expect = 3.9e-92
Identity = 192/227 (84.58%), Postives = 206/227 (90.75%), Query Frame = 0


Query: 1   MHALL--LNSYPYTGAPLTLQTPSSSRTFKISNFFFR-----RRLSRSLRIPLP-NSRSQ 60
           MHA++  LN+ PYT AP TL++PS SR FK+S FFFR     R+  RSLRI LP +SR++
Sbjct: 238 MHAVVWNLNTSPYTRAPQTLESPSCSRNFKVSRFFFRHVSPWRKSCRSLRIRLPSSSRTR 297

Query: 61  VIRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSC 120
           +IRAVAT +SALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGN+VRLKLQGACGSC
Sbjct: 298 LIRAVATPNSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNIVRLKLQGACGSC 357

Query: 121 PSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIRPYLVGAAGGS 180
           PSSV TMKMGIERRLMEKIPEIVAVEPIAD ETGLELNE+NIEKVLEEIRPYLVGAAGGS
Sbjct: 358 PSSVTTMKMGIERRLMEKIPEIVAVEPIADVETGLELNEENIEKVLEEIRPYLVGAAGGS 417

Query: 181 LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPTIAAVQLLS 220
           LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIP IAAVQLLS
Sbjct: 418 LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPAIAAVQLLS 464

[Close]

BLAST of Tan0012661 vs. NCBI nr
Match: XP_038877843.1 (nifU-like protein 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 348.6 bits (893), Expect = 3.9e-92
Identity = 194/226 (85.84%), Postives = 203/226 (89.82%), Query Frame = 0


Query: 1   MHALL--LNSYPYTGAPLTLQTPSSSRTFKISNFFFR-----RRLSRSLRIPLPN-SRSQ 60
           MHA++  LN  PYT  P TL++PS SRTFKIS FF R     RR  RSLR+ LP+ SR++
Sbjct: 1   MHAMVLNLNPSPYTRPPQTLESPSCSRTFKISRFFLRHLSPWRRSCRSLRVRLPSTSRTR 60

Query: 61  VIRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSC 120
           VIRAVAT  SALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSC
Sbjct: 61  VIRAVATPHSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSC 120

Query: 121 PSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIRPYLVGAAGGS 180
           PSSV TMKMGIERRLMEKIPEIVAVEPIADEETGLELNE+NIEKVLEEIRPYLVGAAGGS
Sbjct: 121 PSSVTTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEENIEKVLEEIRPYLVGAAGGS 180

Query: 181 LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPTIAAVQLL 219
           LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIP IAAVQLL
Sbjct: 181 LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPAIAAVQLL 226

[Close]

BLAST of Tan0012661 vs. NCBI nr
Match: XP_022998589.1 (nifU-like protein 2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 347.8 bits (891), Expect = 6.7e-92
Identity = 197/237 (83.12%), Postives = 205/237 (86.50%), Query Frame = 0


Query: 1   MHALLLNSYPYTGAPLTLQTPS------SSRTFKISNFFFR------------RRLSRSL 60
           MH ++LNS PY  AP TLQ+PS      SSR FKIS+FF R            RR  RSL
Sbjct: 1   MHPMVLNSSPYHRAPQTLQSPSSSSSSFSSRAFKISSFFVRHVSPSRQRSLLCRRSCRSL 60

Query: 61  RIPLPNSRSQVIRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVR 120
           RI   NSR+ VIRAVATS+SALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVR
Sbjct: 61  RI---NSRTPVIRAVATSNSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVR 120

Query: 121 LKLQGACGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIR 180
           LKLQGACGSCPSSV TMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIR
Sbjct: 121 LKLQGACGSCPSSVTTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIR 180

Query: 181 PYLVGAAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPTIAAVQLLS 220
           PYL+GAAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIP+IAAVQLLS
Sbjct: 181 PYLIGAAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPSIAAVQLLS 234

[Close]

BLAST of Tan0012661 vs. ExPASy TrEMBL
Match: A0A6J1DC70 (nifU-like protein 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111019691 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.0e-93
Identity = 197/232 (84.91%), Postives = 203/232 (87.50%), Query Frame = 0


Query: 1   MHALLLNSYPYTGAPLTLQTPSSSRTFKISNFFFR------------RRLSRSLRIPLP- 60
           MHA++LNS PYT AP  L+TPSSSR FKIS  F              R+  RS RI LP 
Sbjct: 1   MHAVVLNSSPYTRAPQPLETPSSSRAFKISRPFLSHVSPSRERTPLWRKSCRSFRIRLPT 60

Query: 61  NSRSQVIRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQG 120
           NSR  V+RAVATS+SALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQG
Sbjct: 61  NSRRPVMRAVATSNSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQG 120

Query: 121 ACGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIRPYLVG 180
           ACGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIRPYLVG
Sbjct: 121 ACGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIRPYLVG 180

Query: 181 AAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPTIAAVQLLS 220
           AAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIP IAAVQLLS
Sbjct: 181 AAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPAIAAVQLLS 232

[Close]

BLAST of Tan0012661 vs. ExPASy TrEMBL
Match: A0A5A7SSG4 (NifU-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002690 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.9e-92
Identity = 192/227 (84.58%), Postives = 206/227 (90.75%), Query Frame = 0


Query: 1   MHALL--LNSYPYTGAPLTLQTPSSSRTFKISNFFFR-----RRLSRSLRIPLP-NSRSQ 60
           MHA++  LN+ PYT AP TL++PS SR FK+S FFFR     R+  RSLRI LP +SR++
Sbjct: 238 MHAVVWNLNTSPYTRAPQTLESPSCSRNFKVSRFFFRHVSPWRKSCRSLRIRLPSSSRTR 297

Query: 61  VIRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSC 120
           +IRAVAT +SALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGN+VRLKLQGACGSC
Sbjct: 298 LIRAVATPNSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNIVRLKLQGACGSC 357

Query: 121 PSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIRPYLVGAAGGS 180
           PSSV TMKMGIERRLMEKIPEIVAVEPIAD ETGLELNE+NIEKVLEEIRPYLVGAAGGS
Sbjct: 358 PSSVTTMKMGIERRLMEKIPEIVAVEPIADVETGLELNEENIEKVLEEIRPYLVGAAGGS 417

Query: 181 LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPTIAAVQLLS 220
           LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIP IAAVQLLS
Sbjct: 418 LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPAIAAVQLLS 464

[Close]

BLAST of Tan0012661 vs. ExPASy TrEMBL
Match: A0A1S3BEN3 (nifU-like protein 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103488846 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.9e-92
Identity = 192/227 (84.58%), Postives = 206/227 (90.75%), Query Frame = 0


Query: 1   MHALL--LNSYPYTGAPLTLQTPSSSRTFKISNFFFR-----RRLSRSLRIPLP-NSRSQ 60
           MHA++  LN+ PYT AP TL++PS SR FK+S FFFR     R+  RSLRI LP +SR++
Sbjct: 1   MHAVVWNLNTSPYTRAPQTLESPSCSRNFKVSRFFFRHVSPWRKSCRSLRIRLPSSSRTR 60

Query: 61  VIRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSC 120
           +IRAVAT +SALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGN+VRLKLQGACGSC
Sbjct: 61  LIRAVATPNSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNIVRLKLQGACGSC 120

Query: 121 PSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIRPYLVGAAGGS 180
           PSSV TMKMGIERRLMEKIPEIVAVEPIAD ETGLELNE+NIEKVLEEIRPYLVGAAGGS
Sbjct: 121 PSSVTTMKMGIERRLMEKIPEIVAVEPIADVETGLELNEENIEKVLEEIRPYLVGAAGGS 180

Query: 181 LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPTIAAVQLLS 220
           LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIP IAAVQLLS
Sbjct: 181 LELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPAIAAVQLLS 227

[Close]

BLAST of Tan0012661 vs. ExPASy TrEMBL
Match: A0A6J1KEQ7 (nifU-like protein 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111493175 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.3e-92
Identity = 197/237 (83.12%), Postives = 205/237 (86.50%), Query Frame = 0


Query: 1   MHALLLNSYPYTGAPLTLQTPS------SSRTFKISNFFFR------------RRLSRSL 60
           MH ++LNS PY  AP TLQ+PS      SSR FKIS+FF R            RR  RSL
Sbjct: 1   MHPMVLNSSPYHRAPQTLQSPSSSSSSFSSRAFKISSFFVRHVSPSRQRSLLCRRSCRSL 60

Query: 61  RIPLPNSRSQVIRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVR 120
           RI   NSR+ VIRAVATS+SALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVR
Sbjct: 61  RI---NSRTPVIRAVATSNSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVR 120

Query: 121 LKLQGACGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIR 180
           LKLQGACGSCPSSV TMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIR
Sbjct: 121 LKLQGACGSCPSSVTTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIR 180

Query: 181 PYLVGAAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPTIAAVQLLS 220
           PYL+GAAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIP+IAAVQLLS
Sbjct: 181 PYLIGAAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPSIAAVQLLS 234

[Close]

BLAST of Tan0012661 vs. ExPASy TrEMBL
Match: A0A6J1HP60 (nifU-like protein 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466441 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.6e-91
Identity = 195/226 (86.28%), Postives = 200/226 (88.50%), Query Frame = 0


Query: 1   MHALLLNSYPYTGAPLTLQTPSSSRTFKISNFFFR------RRLSRSLRIP-LPNSRSQV 60
           MHA++LNS P   AP TL TP   R+FKIS   F       RR SRSLR   L NSRSQV
Sbjct: 1   MHAVVLNSSPCARAPQTLGTP-CLRSFKISTLLFAHCSPLWRRSSRSLRNRLLSNSRSQV 60

Query: 61  IRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSCP 120
           IRAVAT DSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSCP
Sbjct: 61  IRAVATPDSALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSCP 120

Query: 121 SSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEIRPYLVGAAGGSL 180
           SSVMTMKMGIERRLMEKIPEIVAVEP+ADEETGLELNE+NIEKVLEEIRPYLVGAAGGSL
Sbjct: 121 SSVMTMKMGIERRLMEKIPEIVAVEPVADEETGLELNEENIEKVLEEIRPYLVGAAGGSL 180

Query: 181 ELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPTIAAVQLLS 220
           ELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIP IAAVQLLS
Sbjct: 181 ELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPVIAAVQLLS 225

[Close]

BLAST of Tan0012661 vs. TAIR 10
Match: AT5G49940.1 (NIFU-like protein 2 )

HSP 1 Score: 281.6 bits (719), Expect = 5.5e-76
Identity = 148/184 (80.43%), Postives = 165/184 (89.67%), Query Frame = 0


Query: 35  RRRLSRSLRIPLPNSRSQVIRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALH 94
           RR LSR L       RS+V++AVAT D  LE+PLT ENVESVLDE+RPYL++DGGNVALH
Sbjct: 52  RRGLSRFLSSRQLFRRSKVVKAVATPDPILEVPLTEENVESVLDEIRPYLMSDGGNVALH 111

Query: 95  EIDGNVVRLKLQGACGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNI 154
           EIDGN+VR+KLQGACGSCPSS MTMKMGIERRLMEKIPEIVAVE + DEETGLELNE+NI
Sbjct: 112 EIDGNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEETGLELNEENI 171

Query: 155 EKVLEEIRPYLVGAAGGSLELVGIEEPIVKVRITGPAAGVMTVRVAVTQKLREKIPTIAA 214
           EKVLEEIRPYL+G A GSL+LV IE+PIVK+RITGPAAGVMTVRVAVTQKLREKIP+IAA
Sbjct: 172 EKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRITGPAAGVMTVRVAVTQKLREKIPSIAA 231

Query: 215 VQLL 219
           VQL+
Sbjct: 232 VQLI 235

[Close]

BLAST of Tan0012661 vs. TAIR 10
Match: AT4G25910.1 (NFU domain protein 3 )

HSP 1 Score: 217.6 bits (553), Expect = 9.8e-57
Identity = 116/154 (75.32%), Postives = 131/154 (85.06%), Query Frame = 0


Query: 66  LPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSCPSSVMTMKMGIER 125
           +PLT ENVE VLDEVRP L+ADGGNVALHEIDG VV LKLQGACGSCPSS MT+KMGIE 
Sbjct: 82  MPLTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIES 141

Query: 126 RLMEKIPEIVAVEPIADEET-GLELNEDNIEKVLEEIRPYLVGAAGGSLELVGIEEPIVK 185
           RL +KIPEI++VE   + ET GLELN++NIEKVL E+RPYL G  GG LELV I+  +VK
Sbjct: 142 RLRDKIPEIMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVK 201

Query: 186 VRITGPAAGVMTVRVAVTQKLREKIPTIAAVQLL 219
           VR+TGPAAGVMTVRVA+TQKLRE IP+I AVQLL
Sbjct: 202 VRLTGPAAGVMTVRVALTQKLRETIPSIGAVQLL 235

[Close]

BLAST of Tan0012661 vs. TAIR 10
Match: AT5G49940.2 (NIFU-like protein 2 )

HSP 1 Score: 185.7 bits (470), Expect = 4.1e-47
Identity = 96/123 (78.05%), Postives = 107/123 (86.99%), Query Frame = 0


Query: 35  RRRLSRSLRIPLPNSRSQVIRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALH 94
           RR LSR L       RS+V++AVAT D  LE+PLT ENVESVLDE+RPYL++DGGNVALH
Sbjct: 52  RRGLSRFLSSRQLFRRSKVVKAVATPDPILEVPLTEENVESVLDEIRPYLMSDGGNVALH 111

Query: 95  EIDGNVVRLKLQGACGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNI 154
           EIDGN+VR+KLQGACGSCPSS MTMKMGIERRLMEKIPEIVAVE + DEETGLELNE+NI
Sbjct: 112 EIDGNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEETGLELNEENI 171

Query: 155 EKV 158
           EKV
Sbjct: 172 EKV 174

[Close]

BLAST of Tan0012661 vs. TAIR 10
Match: AT4G01940.1 (NFU domain protein 1 )

HSP 1 Score: 99.4 bits (246), Expect = 3.9e-21
Identity = 59/147 (40.14%), Postives = 91/147 (61.90%), Query Frame = 0


Query: 62  SALELPLTAENVESVLDEVRPYLIADGGNVALHEIDGNVVRLKLQGACGSCPSSVMTMKM 121
           SA    LT +NV+ VL++VRP+LI+DGGNV +  ++  VV LKLQGAC SCPSS  TM M
Sbjct: 79  SAQTFDLTPQNVDLVLEDVRPFLISDGGNVDVVSVEDGVVSLKLQGACTSCPSSSTTMTM 138

Query: 122 GIERRLMEKIPEIVA-VEPIADEETGLELNEDNIEKVLEEIRPYLVGAAGGSLELVGIEE 181
           GIER L EK  + +  +  + DEE   ++  + +   L+ +RP  +   GGS+E++ +E 
Sbjct: 139 GIERVLKEKFGDALKDIRQVFDEEV-KQITVEAVNAHLDILRP-AIKNYGGSVEVLSVEG 198

Query: 182 PIVKVRITGPAAGVMTVRVAVTQKLRE 208
               V+  GP +  M ++ A+ +K ++
Sbjct: 199 EDCVVKYVGPESIGMGIQAAIKEKFKD 223

[Close]

BLAST of Tan0012661 vs. TAIR 10
Match: AT3G20970.1 (NFU domain protein 4 )

HSP 1 Score: 55.5 bits (132), Expect = 6.4e-08
Identity = 35/96 (36.46%), Postives = 50/96 (52.08%), Query Frame = 0


Query: 51  SQVIRAVATSDSALELPLTAENVESVLDEVRPYLIADGGNVALHEID--GNVVRLKLQGA 110
           SQ   A  T+ S  +    A   E +   +RP +  DGG++     D    +V+L++QGA
Sbjct: 175 SQAAAAKDTAISEDDSETVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGA 234

Query: 111 CGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEE 145
           C  CPSS +T+K GIE  LM  + E+  VE   D E
Sbjct: 235 CSGCPSSSVTLKSGIENMLMHYVSEVKGVEQEFDGE 270

The following BLAST results are available for this feature:

BLAST of Tan0012661 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93W20	7.8e-75	80.43	NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1...	[more]
Q84LK7	5.8e-62	63.55	NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=N...	[more]
Q84RQ7	1.4e-55	75.32	NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2...	[more]
Q93W77	5.5e-20	40.14	NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1...	[more]
O32119	4.8e-16	52.86	Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224...	[more]

BLAST of Tan0012661 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151795.1	2.1e-93	84.91	nifU-like protein 2, chloroplastic [Momordica charantia]	[more]
XP_008445979.1	3.9e-92	84.58	PREDICTED: nifU-like protein 2, chloroplastic [Cucumis melo]	[more]
KAA0034154.1	3.9e-92	84.58	nifU-like protein 2 [Cucumis melo var. makuwa] >TYK15765.1 nifU-like protein 2 [...	[more]
XP_038877843.1	3.9e-92	85.84	nifU-like protein 2, chloroplastic [Benincasa hispida]	[more]
XP_022998589.1	6.7e-92	83.12	nifU-like protein 2, chloroplastic [Cucurbita maxima]	[more]

BLAST of Tan0012661 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DC70	1.0e-93	84.91	nifU-like protein 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11101969...	[more]
A0A5A7SSG4	1.9e-92	84.58	NifU-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G0...	[more]
A0A1S3BEN3	1.9e-92	84.58	nifU-like protein 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103488846 PE=3 ...	[more]
A0A6J1KEQ7	3.3e-92	83.12	nifU-like protein 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111493175 P...	[more]
A0A6J1HP60	3.6e-91	86.28	nifU-like protein 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466441 P...	[more]

BLAST of Tan0012661 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G49940.1	5.5e-76	80.43	NIFU-like protein 2	[more]
AT4G25910.1	9.8e-57	75.32	NFU domain protein 3	[more]
AT5G49940.2	4.1e-47	78.05	NIFU-like protein 2	[more]
AT4G01940.1	3.9e-21	40.14	NFU domain protein 1	[more]
AT3G20970.1	6.4e-08	36.46	NFU domain protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001075	NIF system FeS cluster assembly, NifU, C-terminal	PFAM	PF01106	NifU	coord: 154..216 e-value: 2.4E-13 score: 50.0 coord: 73..138 e-value: 6.4E-27 score: 93.5
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 64..147 e-value: 3.2E-34 score: 118.8
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 148..219 e-value: 5.9E-22 score: 79.6
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 70..144
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 148..217
None	No IPR available	PANTHER	PTHR11178	IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED	coord: 35..218
None	No IPR available	PANTHER	PTHR11178:SF39	FIXATION PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 35..218

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0012661.1	Tan0012661.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0051536	iron-sulfur cluster binding