Tan0012556 (gene) Snake gourd v1

Overview


Name	Tan0012556
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	E3 ubiquitin-protein ligase Praja-2
Location	LG03: 62251494 .. 62255172 (-)
RNA-Seq Expression	Tan0012556
Synteny	Tan0012556

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATTAGATCAAAGCATGAACACCCAGATACAGAATTCAATCCATCACTGTCCAAATTTTGTGAGAAGAATGTGAGTGGGTCAATTACGCTGCTCGCTTTCAAGCGCGTTTTGATTTGTTGAAAGTTCTTCGTTTTCCATCCTCCAATGGCGGCCGCGCCCTCAAATCGCTTTTCTCGAAGAACCCCATTGGCCATCTCACTGGTTTTCGTTTTCCCCCTCTGATTTCTGTTCATCTTCGTACCGTTTTCACCGACCCACGACGGATGTCTCTGAACAGCGTTCTTCAATTATTTGTCTCTCTTGTCCTTTTTGCCCAATCGCTTTGCCCTTTTCCTTGATTGTCTTATTTTCTTCGTTTTCCCCGATTTTTTGGATCGTTTTCTTCTATTGGATGAGCTCCAGCCGAATGATGAAGTTTTGAAGGACTGAAAGCGAAGTTACTGGTAATAATCGCAACCCATCTACGTCGTTCTCGATTCTGTTGATGCAGTTTTTCGTTTTCCCTAATTGTATGTAATGCCATTTTTGTGTCTCTCTGCGCCAATCGTTTGTTTTCTGATTTGGGATTTTGGTCTTTGTCTCGCTAAATTGATACTAGAGGGAGAACAGTAGTAGTAAGAATCGTGCATTGAGTGGTTGACTCGTCTTCTTCTAATGCGATTGTATTGAAATTGAAAGCCTCTAGTTTAGACGAAACCATTTGTTCCCTTTTGTCTGGTTTACTGATCGAATTCTGTAACCTCTCTTGAACCCTTAAAATTTATGATAGTGACTGTTAAGTAGTTTTTTGAATTTAGAATTTGGCTAAGAATTCAAATCCTTCCTTAATAGAGATAAAAATTATTGTAGAAAAATTGGAATAAAACAAGCTTAATTTTCAAAAACCAAATGGTTATCAAACAGGTTACAGTTATTGAGATTGAGTTTGTGTTGTTTGAGGGTTTTAGCATAAAAATTAAGGATGGACCTGTGGCTGACATTGGTTCTTTATGAACTCCCAACATGGTTTGAGAAGATTCTTCCGTCATTCACCTTCTTTTCTACCTTCAAAGTTGAGTGTTGACCAATTCTTTTCACTCCTGGGGAGGGGCAATGGAGGGAGGCTGGACTATATCATGATGTTGATGAAAGGGCATTTCAATTTGGATGGAGATAAGAATATGGTTTAGTTTGAAATGAAGGGAGTGAAGTGAGATTAGGAATGCAGTGGTAAAGGAATGTTCTCTCTTTTAAATCTGTTCTCAACTAGGGGTTGTGGAGGGTGGGGAGTAGTTGTCATTTGTCAATGTACTATAGGAAACACGGACAAGGAGCGGCCCATGACAGGTTTCTGAGCGAAGGAATAGATAATGAAACGGTAGCAGTTGAGAATGTGAGTTGAGTTTGAGATAGTGCAAGTACAAAACAACTTAGTTAAAACTTGAGAGCTTGAGTACATGGCTACAAGTGCAGAAAACTATGGCTAGGCAGTGTTTAATTGAACCAGTCTGGTCAGTTTCTCAAGTCGTTTGATTGTCTCTGATTTAAAATTCTCTGTATGACTTGCATTGTTTTCCATCTTTGACTGGCCTTCAACTTAGTAAATTTGATTCGATCTCTCTTATAGGTTATTGTTTCACCACTTCTTGAACATATTGGTTTTCCTCTGTTCATTATTTGTGCTTGCTGATCTCATCTCAGATGAACATTGACTCACAAAGAATCTTGGAGGAGTTTCCTCAATCCACTGCTCGTGTGATGGACAGCAATCAACGAGTCGATGAACATGGTCCAATTCAGCCGCCAACTTGTGCATGTTGCCAACGAGTCCTAGTACCTGATAATGATGCCACTGTTGAACTTCAAGACATTGGTATATGTGGCGATTGTAAATTTTTGTTACTCGAAGATATCGAAACCCCTGTACGAGATTCTTATCACAGGATGACGCCTAGAGGAAGAAGAAGACATGGTAGTTCAGAGTCCGGTGAAAATCTCTTTTCACAGGAATTCACCCATATGATTAACTTGGTGAGGCAAAGTCAATCCAGCATCTCTGGCCACGGGGATCGATATGTAGATGGTTCTAATGCTGCTGATGTCATGCAGCACTCGAGCTCCCATACGACCCCTAATCAATCTAGAAGATGGCGGCAAGTATACTCGGATTCTGAAAATGATGGTTTAGATAGTATTGACTCTATGTTTGGGGAGAGTGAATCAAATTTCAGTTTTGGGCCTTACAGACATTCTTATGGTGATAGTGATGCTGTTTCTTATAGAACTTATGGGGGTGACTCTGATGCTTCCATGGATGGACATAGTTTCTTGGACACGGACATTTTCGTTATGCCAGATGACGAATCCGACGATACTGATACAGACATTGATCCCATGCATGCTGGTGTCGGCCATTGGGACTCGAGTGACGAGGAGGATGATCTTGGTGAATTAACGGAAGCTGACACAGAGCAAGATGCACTTGAAGTAGAATCTTCTGAAGCTACACCTCAACTTCAAAATTTTCATGTTTCTAGTGCTACTGGGAGAGGTAACTCTGGTAATTGGCATGAACAACTTTCTTCACCAGAATATGAACCCGGGCAAATCAGGAGGAACAGGCGATTTTATTCTATCACCGACTATGCACGGTCAGAATTACTATCTTTTGTTGGGGATTCTGGTGACTATCTTGACCAACAAGGCTTTGAAGAACTGCTCGAGCAAATTGCCGAGACAACCAGCGCAAGACGTGGAGCACCTCCTGCAGCTGTCTCTTCGGTGAAAAATCTCCCACGTTTGGTGGTTACTAATGAACATTTGAAGCATGACAATATATCCTGTGCAATTTGCAAGGATTTCTTACTTGTAGGCGTCGAAGTCAATCAACTGCCTTGCCTTCATCTCTACCACCCTTTCTGCATCTTGCCGTGGTTGAGTGCACGGAATTCGTGCCCCCTTTGTCGCTATGAACTTCCAACCGATGATCGGGATTACGAAGAGGGAAAGCGAAGCAGTATCAATACTGCAGAGATCCAAGGACTTCGGCATCGAGTAGTGGATCTCGAAAGTCCCAGCAACATAAATGGAGTTGGAGCTAATCAAGAGTTTGAAACCAGTCAAGGAGAGCAGCAGAGCAACATAGACTCTGCAGTTAATAATAGTAACAATTCAGGCAGACACGGAGGTAGAAGACGTTGGCTGTTCGTCGCTGCTCCGATAGTTGGTCTTGTAGGTATAGCTCTGATGTTATGGTTTGGCAACCCTCTATGTGATCAAAGAGTTCCGGGAGGTCGATTTACAGACAGAAGGCAGCTTGATGCTCATATTGCAGATGCATCAAACCAAAGAGAGAACAGAACCAGGAGATGGTGGTCATTCGTCTAGAAATCGCCAAGTTCGGTATGGAAAATCGAATTAACACGAGCTTTCTTCGTTTTGTTGATTTCCCAGGATTGAAATCTAAGGTTTTCAGCTTGTAGTTCAGTAAATTTTCGGTTTTTCGTTGAAGTGTTGAAGTAGAAGAGTTAATTTTTAACCCACAAAAGCAACTTATGCCTTGCAATGGGGGCAGTAATAAACAGGTCAATTGGTTCGATTGTGAATGTGTATACTTTCTTACAGTTTATGTAAATAGAAATGTATATTTGATTCAAACGGATTTGTTTTATATAATGATCATCGAATTCCATTCCAAG

mRNA sequence

CAATTAGATCAAAGCATGAACACCCAGATACAGAATTCAATCCATCACTGTCCAAATTTTGTGAGAAGAATGTGAGTGGGTCAATTACGCTGCTCGCTTTCAAGCGCGTTTTGATTTGTTGAAAGTTCTTCGTTTTCCATCCTCCAATGGCGGCCGCGCCCTCAAATCGCTTTTCTCGAAGAACCCCATTGGCCATCTCACTGGTTTTCGTTTTCCCCCTCTGATTTCTGTTCATCTTCGTACCGTTTTCACCGACCCACGACGGATGTCTCTGAACAGCGTTCTTCAATTATTTGTCTCTCTTGTCCTTTTTGCCCAATCGCTTTGCCCTTTTCCTTGATTGTCTTATTTTCTTCGTTTTCCCCGATTTTTTGGATCGTTTTCTTCTATTGGATGAGCTCCAGCCGAATGATGAAGTTTTGAAGGACTGAAAGCGAAGTTACTGATGAACATTGACTCACAAAGAATCTTGGAGGAGTTTCCTCAATCCACTGCTCGTGTGATGGACAGCAATCAACGAGTCGATGAACATGGTCCAATTCAGCCGCCAACTTGTGCATGTTGCCAACGAGTCCTAGTACCTGATAATGATGCCACTGTTGAACTTCAAGACATTGGTATATGTGGCGATTGTAAATTTTTGTTACTCGAAGATATCGAAACCCCTGTACGAGATTCTTATCACAGGATGACGCCTAGAGGAAGAAGAAGACATGGTAGTTCAGAGTCCGGTGAAAATCTCTTTTCACAGGAATTCACCCATATGATTAACTTGGTGAGGCAAAGTCAATCCAGCATCTCTGGCCACGGGGATCGATATGTAGATGGTTCTAATGCTGCTGATGTCATGCAGCACTCGAGCTCCCATACGACCCCTAATCAATCTAGAAGATGGCGGCAAGTATACTCGGATTCTGAAAATGATGGTTTAGATAGTATTGACTCTATGTTTGGGGAGAGTGAATCAAATTTCAGTTTTGGGCCTTACAGACATTCTTATGGTGATAGTGATGCTGTTTCTTATAGAACTTATGGGGGTGACTCTGATGCTTCCATGGATGGACATAGTTTCTTGGACACGGACATTTTCGTTATGCCAGATGACGAATCCGACGATACTGATACAGACATTGATCCCATGCATGCTGGTGTCGGCCATTGGGACTCGAGTGACGAGGAGGATGATCTTGGTGAATTAACGGAAGCTGACACAGAGCAAGATGCACTTGAAGTAGAATCTTCTGAAGCTACACCTCAACTTCAAAATTTTCATGTTTCTAGTGCTACTGGGAGAGGTAACTCTGGTAATTGGCATGAACAACTTTCTTCACCAGAATATGAACCCGGGCAAATCAGGAGGAACAGGCGATTTTATTCTATCACCGACTATGCACGGTCAGAATTACTATCTTTTGTTGGGGATTCTGGTGACTATCTTGACCAACAAGGCTTTGAAGAACTGCTCGAGCAAATTGCCGAGACAACCAGCGCAAGACGTGGAGCACCTCCTGCAGCTGTCTCTTCGGTGAAAAATCTCCCACGTTTGGTGGTTACTAATGAACATTTGAAGCATGACAATATATCCTGTGCAATTTGCAAGGATTTCTTACTTGTAGGCGTCGAAGTCAATCAACTGCCTTGCCTTCATCTCTACCACCCTTTCTGCATCTTGCCGTGGTTGAGTGCACGGAATTCGTGCCCCCTTTGTCGCTATGAACTTCCAACCGATGATCGGGATTACGAAGAGGGAAAGCGAAGCAGTATCAATACTGCAGAGATCCAAGGACTTCGGCATCGAGTAGTGGATCTCGAAAGTCCCAGCAACATAAATGGAGTTGGAGCTAATCAAGAGTTTGAAACCAGTCAAGGAGAGCAGCAGAGCAACATAGACTCTGCAGTTAATAATAGTAACAATTCAGGCAGACACGGAGGTAGAAGACGTTGGCTGTTCGTCGCTGCTCCGATAGTTGGTCTTGTAGGTATAGCTCTGATGTTATGGTTTGGCAACCCTCTATGTGATCAAAGAGTTCCGGGAGGTCGATTTACAGACAGAAGGCAGCTTGATGCTCATATTGCAGATGCATCAAACCAAAGAGAGAACAGAACCAGGAGATGGTGGTCATTCGTCTAGAAATCGCCAAGTTCGGTATGGAAAATCGAATTAACACGAGCTTTCTTCGTTTTGTTGATTTCCCAGGATTGAAATCTAAGGTTTTCAGCTTGTAGTTCAGTAAATTTTCGGTTTTTCGTTGAAGTGTTGAAGTAGAAGAGTTAATTTTTAACCCACAAAAGCAACTTATGCCTTGCAATGGGGGCAGTAATAAACAGGTCAATTGGTTCGATTGTGAATGTGTATACTTTCTTACAGTTTATGTAAATAGAAATGTATATTTGATTCAAACGGATTTGTTTTATATAATGATCATCGAATTCCATTCCAAG

Coding sequence (CDS)

ATGAACATTGACTCACAAAGAATCTTGGAGGAGTTTCCTCAATCCACTGCTCGTGTGATGGACAGCAATCAACGAGTCGATGAACATGGTCCAATTCAGCCGCCAACTTGTGCATGTTGCCAACGAGTCCTAGTACCTGATAATGATGCCACTGTTGAACTTCAAGACATTGGTATATGTGGCGATTGTAAATTTTTGTTACTCGAAGATATCGAAACCCCTGTACGAGATTCTTATCACAGGATGACGCCTAGAGGAAGAAGAAGACATGGTAGTTCAGAGTCCGGTGAAAATCTCTTTTCACAGGAATTCACCCATATGATTAACTTGGTGAGGCAAAGTCAATCCAGCATCTCTGGCCACGGGGATCGATATGTAGATGGTTCTAATGCTGCTGATGTCATGCAGCACTCGAGCTCCCATACGACCCCTAATCAATCTAGAAGATGGCGGCAAGTATACTCGGATTCTGAAAATGATGGTTTAGATAGTATTGACTCTATGTTTGGGGAGAGTGAATCAAATTTCAGTTTTGGGCCTTACAGACATTCTTATGGTGATAGTGATGCTGTTTCTTATAGAACTTATGGGGGTGACTCTGATGCTTCCATGGATGGACATAGTTTCTTGGACACGGACATTTTCGTTATGCCAGATGACGAATCCGACGATACTGATACAGACATTGATCCCATGCATGCTGGTGTCGGCCATTGGGACTCGAGTGACGAGGAGGATGATCTTGGTGAATTAACGGAAGCTGACACAGAGCAAGATGCACTTGAAGTAGAATCTTCTGAAGCTACACCTCAACTTCAAAATTTTCATGTTTCTAGTGCTACTGGGAGAGGTAACTCTGGTAATTGGCATGAACAACTTTCTTCACCAGAATATGAACCCGGGCAAATCAGGAGGAACAGGCGATTTTATTCTATCACCGACTATGCACGGTCAGAATTACTATCTTTTGTTGGGGATTCTGGTGACTATCTTGACCAACAAGGCTTTGAAGAACTGCTCGAGCAAATTGCCGAGACAACCAGCGCAAGACGTGGAGCACCTCCTGCAGCTGTCTCTTCGGTGAAAAATCTCCCACGTTTGGTGGTTACTAATGAACATTTGAAGCATGACAATATATCCTGTGCAATTTGCAAGGATTTCTTACTTGTAGGCGTCGAAGTCAATCAACTGCCTTGCCTTCATCTCTACCACCCTTTCTGCATCTTGCCGTGGTTGAGTGCACGGAATTCGTGCCCCCTTTGTCGCTATGAACTTCCAACCGATGATCGGGATTACGAAGAGGGAAAGCGAAGCAGTATCAATACTGCAGAGATCCAAGGACTTCGGCATCGAGTAGTGGATCTCGAAAGTCCCAGCAACATAAATGGAGTTGGAGCTAATCAAGAGTTTGAAACCAGTCAAGGAGAGCAGCAGAGCAACATAGACTCTGCAGTTAATAATAGTAACAATTCAGGCAGACACGGAGGTAGAAGACGTTGGCTGTTCGTCGCTGCTCCGATAGTTGGTCTTGTAGGTATAGCTCTGATGTTATGGTTTGGCAACCCTCTATGTGATCAAAGAGTTCCGGGAGGTCGATTTACAGACAGAAGGCAGCTTGATGCTCATATTGCAGATGCATCAAACCAAAGAGAGAACAGAACCAGGAGATGGTGGTCATTCGTCTAG

Protein sequence

MNIDSQRILEEFPQSTARVMDSNQRVDEHGPIQPPTCACCQRVLVPDNDATVELQDIGICGDCKFLLLEDIETPVRDSYHRMTPRGRRRHGSSESGENLFSQEFTHMINLVRQSQSSISGHGDRYVDGSNAADVMQHSSSHTTPNQSRRWRQVYSDSENDGLDSIDSMFGESESNFSFGPYRHSYGDSDAVSYRTYGGDSDASMDGHSFLDTDIFVMPDDESDDTDTDIDPMHAGVGHWDSSDEEDDLGELTEADTEQDALEVESSEATPQLQNFHVSSATGRGNSGNWHEQLSSPEYEPGQIRRNRRFYSITDYARSELLSFVGDSGDYLDQQGFEELLEQIAETTSARRGAPPAAVSSVKNLPRLVVTNEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPFCILPWLSARNSCPLCRYELPTDDRDYEEGKRSSINTAEIQGLRHRVVDLESPSNINGVGANQEFETSQGEQQSNIDSAVNNSNNSGRHGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGRFTDRRQLDAHIADASNQRENRTRRWWSFV

Homology

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BLAST of Tan0012556 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.8e-27
Identity = 56/123 (45.53%), Postives = 78/123 (63.41%), Query Frame = 0


Query: 325 GDSGDYLDQQGFEELLEQIAETTSARRGAPPAAVSSVKNLPRLVVTNEHLKHDNISCAIC 384
           G+ GDY    G E+L++Q+AE    R G PPA+ S++  LP + VT + LK +   CA+C
Sbjct: 160 GNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVC 219

Query: 385 KDFLLVGVEVNQLPCLHLYHPFCILPWLSARNSCPLCRYELPTDDRDYEEGKRSSINTAE 444
            D    G +V Q+PC H++H  C+LPWL   NSCP+CR+ELPTDD DYE   + S  + +
Sbjct: 220 MDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGD 279

Query: 445 IQG 448
            QG
Sbjct: 280 GQG 282

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BLAST of Tan0012556 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 1.2e-26
Identity = 54/116 (46.55%), Postives = 73/116 (62.93%), Query Frame = 0


Query: 328 GDYLDQQGFEELLEQIAETTSARRGAPPAAVSSVKNLPRLVVTNEHLKHDNISCAICKDF 387
           GDY    G E+L++Q+AE    R G PPA+ S+++ LP + +T  +L  +   CA+C D 
Sbjct: 171 GDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDD 230

Query: 388 LLVGVEVNQLPCLHLYHPFCILPWLSARNSCPLCRYELPTDDRDYEEGKRSSINTA 444
              G E  Q+PC HLYH  C+LPWL   NSCP+CR+ELPTDD DYE   R +  T+
Sbjct: 231 FEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTS 286

[Close]

BLAST of Tan0012556 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 2.7e-23
Identity = 55/139 (39.57%), Postives = 76/139 (54.68%), Query Frame = 0


Query: 325 GDSGDYLDQQGFEELLEQIAETTSARRGAPPAAVSSVKNLPRLVVTNEHLKHDNISCAIC 384
           G++GDY    G EEL EQ++  T+ RRG PPA  S++  LP + +   HL+  + +C +C
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTT-RRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 385 KDFLLVGVEVNQLPCLHLYHPFCILPWLSARNSCPLCRYELP------------TDDRDY 444
           KD   +G E  Q+PC H+YH  CI+PWL   NSCP+CR ELP            T  R+Y
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNY 254

Query: 445 EEGKRSSINTAEIQGLRHR 452
                SS + +   G   R
Sbjct: 255 RSSSSSSSSNSRENGNERR 272

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BLAST of Tan0012556 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 5.1e-22
Identity = 77/229 (33.62%), Postives = 111/229 (48.47%), Query Frame = 0


Query: 219 DDESDDTDTDIDPMHAGVGHWDSSDEEDDLGELTEADTEQDALEVESSEATPQLQNFHVS 278
           DD+ D+ + D        G  +  DEE++L    E D E D         T         
Sbjct: 118 DDDEDEDEDD--------GDEEEEDEEENLTVNDEEDEEDDLRRRNRFPLTT------TQ 177

Query: 279 SATGRGNSGNWHEQLSSPEYEPGQIRRNRRFYSITDYARSELLSFVGDSGDYLDQ-QGFE 338
           S TGR    +W E L   E    + R     Y+             G+  DY+D   G+E
Sbjct: 178 SRTGRNRILDWAEILMGIEDNSIEFRMESDRYA-------------GNPADYIDDAAGYE 237

Query: 339 ELLEQIAETT----SARRGAPPAAVSSVKNLPRLVVTNEHLKHDNISCAICKDFLLVGVE 398
            LL+ +AE        RRGAPPAA S+++ L    V++   +   + CA+CKD +++G  
Sbjct: 238 ALLQNLAEGDGGGGGGRRGAPPAAKSAIEALETFEVSSSEGEMVMV-CAVCKDGMVMGET 297

Query: 399 VNQLPCLHLYHPFCILPWLSARNSCPLCRYELPTDDRDYEEGKRSSINT 443
             +LPC H YH  CI+PWL  RNSCP+CR++L TDD +YEE ++   +T
Sbjct: 298 GKKLPCGHCYHGDCIVPWLGTRNSCPVCRFQLETDDAEYEEERKKRTST 318

[Close]

BLAST of Tan0012556 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 8.8e-22
Identity = 48/102 (47.06%), Postives = 65/102 (63.73%), Query Frame = 0


Query: 326 DSGDYLDQQGFEELLEQIAETTSARRGAPPAAVSSVKNLPRLVVTNEHLKHDNISCAICK 385
           ++GDY    G EEL+EQ++  T   RG PPA  SS+  LP + +T +HLK  +  C +CK
Sbjct: 132 NAGDYFFGPGLEELIEQLSSGTH-HRGPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCK 191

Query: 386 DFLLVGVEVNQLPCLHLYHPFCILPWLSARNSCPLCRYELPT 428
           D   +  E  Q+PC H+YH  CI+PWL   NSCP+CR ELP+
Sbjct: 192 DEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPS 232

[Close]

BLAST of Tan0012556 vs. NCBI nr
Match: XP_038882118.1 (E3 ubiquitin-protein ligase Praja-2 [Benincasa hispida] >XP_038882119.1 E3 ubiquitin-protein ligase Praja-2 [Benincasa hispida])

HSP 1 Score: 936.4 bits (2419), Expect = 1.1e-268
Identity = 480/564 (85.11%), Postives = 510/564 (90.43%), Query Frame = 0


Query: 1   MNIDSQRILEEFPQSTARV-MDSNQRVDEHGPIQPPTCACCQRVLVPDNDATVELQDIGI 60
           M+ID QRILEEFPQSTA V M+SNQRVDEH PIQ P+CA CQRVLVPDNDAT E + I +
Sbjct: 1   MDIDLQRILEEFPQSTAHVIMNSNQRVDEHSPIQLPSCAFCQRVLVPDNDATGEPEAIDV 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRR-RHGSSESGENLFSQEFTHMINLVRQSQSSI 120
           CGDCKFLLLED+ETPVRDSYHR T RGRR R+GS ES ENLFSQEFTHMINLVRQ QSSI
Sbjct: 61  CGDCKFLLLEDVETPVRDSYHRTTSRGRRTRYGSVESAENLFSQEFTHMINLVRQRQSSI 120

Query: 121 SGHGDRYVDGSNAADVMQHSSSHTTPNQSRRWRQVYSDSENDGLDSIDSMFGESESNFSF 180
           S HGDRY DGSNAAD MQHSSSHTTPNQSRRWRQVYSDSENDGLDS DSMFGESESNFSF
Sbjct: 121 SSHGDRYADGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASMDGHSFLDTDIFVMPDDESD-DTDTDIDPMHAGVG 240
           GPYRHSYGDSDAVSYRTYGGDSDAS+DGHSFLDTDIFV+P + SD DTDTDIDPM AGVG
Sbjct: 181 GPYRHSYGDSDAVSYRTYGGDSDASLDGHSFLDTDIFVLPGEGSDVDTDTDIDPMRAGVG 240

Query: 241 HWDSSDEEDDLGELTEADTEQDALEVESSEATPQLQNFHVSSATGRGNSGNWHEQLSSPE 300
           HWDSSDEEDD GE TEADTEQ   EVESSEA PQLQNFHVSSA GRG S NW+EQ SSPE
Sbjct: 241 HWDSSDEEDDHGEFTEADTEQ---EVESSEARPQLQNFHVSSAPGRGISSNWNEQFSSPE 300

Query: 301 YE---PGQIRRNRRFYSITDYARSELLSFVGDSGDYLDQQGFEELLEQIAETTSARRGAP 360
           YE   PGQIRRNRRFYSIT+YA+SELLS++GDSG+YLD+Q FEELLEQIAETTS+RRGAP
Sbjct: 301 YEGSAPGQIRRNRRFYSITNYAQSELLSYIGDSGEYLDRQVFEELLEQIAETTSSRRGAP 360

Query: 361 PAAVSSVKNLPRLVVTNEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPFCILPWLSA 420
           PAA+S VKNLPRLV++ EHLKHD ISCAICKDFLLVGVEVNQLPCLHLYHP CILPWLSA
Sbjct: 361 PAAISIVKNLPRLVISKEHLKHDTISCAICKDFLLVGVEVNQLPCLHLYHPSCILPWLSA 420

Query: 421 RNSCPLCRYELPTDDRDYEEGKRSSINTAEIQGLRHRVVDLESPSNINGVGANQEFETSQ 480
           RNSCPLCRYELPTDDRDYEE KRSSINTAEI GL+ RVV LE+P ++N VGA+  FETSQ
Sbjct: 421 RNSCPLCRYELPTDDRDYEEVKRSSINTAEIHGLQPRVVSLENPRDLN-VGASPAFETSQ 480

Query: 481 GEQQSNIDSAVNNSNNSGRHGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGRFT 540
           GE Q+NIDSAVNNSNNSGR GGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRV GG+FT
Sbjct: 481 GEDQTNIDSAVNNSNNSGR-GGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQFT 540

Query: 541 DRRQLDAHIADASNQRENRTRRWW 559
           DRRQL+A IAD+SNQRENRTRRWW
Sbjct: 541 DRRQLNARIADSSNQRENRTRRWW 559

[Close]

BLAST of Tan0012556 vs. NCBI nr
Match: XP_004142724.1 (E3 ubiquitin-protein ligase Praja-2 [Cucumis sativus] >KGN54476.1 hypothetical protein Csa_012302 [Cucumis sativus])

HSP 1 Score: 897.1 bits (2317), Expect = 7.6e-257
Identity = 463/568 (81.51%), Postives = 496/568 (87.32%), Query Frame = 0


Query: 1   MNIDSQRILEEFPQSTARV-MDSNQRVDEHGPIQPPTCACCQRVLVPDNDATVELQDIGI 60
           M+ID QRILEEFPQS   V M++NQRVDEH PIQ PTCA CQRVLVPD+DA  E + I I
Sbjct: 1   MDIDLQRILEEFPQSNVHVIMNNNQRVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRR-RHGSSESGENLFSQEFTHMINLVRQSQSSI 120
           CGDCKFLLLEDIETPVRDSYHRMTPRGRR    SSES ENLFSQEFTHMIN+VRQ QSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120

Query: 121 SGHGDRYVDGSNAADVMQHSSSHTTPNQSRRWRQVYSDSENDGLDSIDSMFGESESNFSF 180
           S HGDRY DGSNA D MQHSSSHTTPNQSRRWRQVYSDSENDGLDS DSMF ESESNFSF
Sbjct: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASM-DGHSFLDTDIFVMPDDESDDTDTDIDPMHAGVG 240
           GPYRHSYGDSD VS RTYGG+SD S+ DGHSFLDTD+F++P  E  DTDTDIDPM AGVG
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGESDVSLDDGHSFLDTDVFILP-GEGSDTDTDIDPMRAGVG 240

Query: 241 HWDSSDEEDDLGELTEADTEQDALEVESSEATPQLQNFHVSSATGRGNSGNWHEQLSSPE 300
           HWDSSDEEDD GE TEA+TEQ   EV SSEA PQLQNFHVSSA+ RGNS NW+EQLSSPE
Sbjct: 241 HWDSSDEEDDHGEFTEAETEQ---EVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPE 300

Query: 301 YE---PGQIRRNRRFYSITDYARSELLSFVGDSGDYLDQQGFEELLEQIAE-TTSARRGA 360
           YE   PG IRRNRRFYSIT+YA+SELLS+VGDSGDYLD+QGFEELLEQIAE TTS+RRGA
Sbjct: 301 YEGSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGA 360

Query: 361 PPAAVSSVKNLPRLVVTNEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPFCILPWLS 420
           PPAAVS VKNLPRLV++ EHLKHD+ISCAICKDFL +GVEVNQLPCLHLYHP CILPWLS
Sbjct: 361 PPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLS 420

Query: 421 ARNSCPLCRYELPTDDRDYEEGKRSSINTAEIQGLRHRVVDLESPSNINGVGANQEFETS 480
           ARNSCPLCRYELPTDDRDYEE K+SSIN A + GL+ RVV LE+PS +NGVGAN EFETS
Sbjct: 421 ARNSCPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETS 480

Query: 481 QGEQQSNIDSAVNNSNNSGRHGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGRF 540
           Q E Q NIDSAV +SNNSGR G RRRWLFVAAPIVGLVGIALMLWFGNPLCDQRV GG+ 
Sbjct: 481 QEEDQRNIDSAVISSNNSGR-GARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQL 540

Query: 541 TDRRQLDAHIADASNQRENRTRRWWSFV 562
           TD RQLDAH A++SNQRENR+RRWW  V
Sbjct: 541 TDGRQLDAHSAESSNQRENRSRRWWQIV 563

[Close]

BLAST of Tan0012556 vs. NCBI nr
Match: XP_008463301.1 (PREDICTED: uncharacterized protein LOC103501491 [Cucumis melo] >TYK06209.1 E3 ubiquitin-protein ligase Praja-2 [Cucumis melo var. makuwa])

HSP 1 Score: 895.2 bits (2312), Expect = 2.9e-256
Identity = 463/563 (82.24%), Postives = 493/563 (87.57%), Query Frame = 0


Query: 1   MNIDSQRILEEFPQSTARV-MDSNQRVDEHGPIQPPTCACCQRVLVPDNDATVELQDIGI 60
           M+ID QRILEEFPQS   V M+SNQRVDEH PIQ PTCA CQRVL  D+DAT E + I I
Sbjct: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRR-RHGSSESGENLFSQEFTHMINLVRQSQSSI 120
           CGDCKFLLLEDIETPVRDSYHRMTPRGRR    SSES ENLFSQEFTHMINLVRQ QSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSI 120

Query: 121 SGHGDRYVDGSNAADVMQHSSSHTTPNQSRRWRQVYSDSENDGLDSIDSMFGESESNFSF 180
           S HGDRY DGSNA D MQHSSSHTTPNQSRRWRQVYSDSENDGLDS DSMFGESESNFSF
Sbjct: 121 SSHGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASMDGHSFLDTDIFVMPDDESDDTDTDIDPMHAGVGH 240
           GPYRHSYGDSD VS RTYGGDSDAS+DGHSFLDTDIF++P  E  DTDTDIDPM AG+G+
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILP-GEGSDTDTDIDPMRAGIGN 240

Query: 241 WDSSDEEDDLGELTEADTEQDALEVESSEATPQLQNFHVSSATGRGNSGNWHEQLSSPEY 300
           WDSSDEEDD GE TEA+TEQ   E+ SSEA PQLQN +VSSA+ RGNS NW EQLSSPEY
Sbjct: 241 WDSSDEEDDHGEFTEAETEQ---EIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEY 300

Query: 301 E---PGQIRRNRRFYSITDYARSELLSFVGDSGDYLDQQGFEELLEQIAETTSARRGAPP 360
           E   PG IRRNRRFYSIT+YA+SELLS+VGDSGDYLD+QGFE+LLEQIAETTS+RRGAPP
Sbjct: 301 EGSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAETTSSRRGAPP 360

Query: 361 AAVSSVKNLPRLVVTNEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPFCILPWLSAR 420
           AAVS VKNLPRLV++ EHLKHD+ISCAICKDFLLVGVEVNQLPCLHLYHP CILPWLSAR
Sbjct: 361 AAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSAR 420

Query: 421 NSCPLCRYELPTDDRDYEEGKRSSINTAEIQGLRHRVVDLESPSNINGVGANQEFETSQG 480
           NSCPLCRYELPTDDRDYEE KRSSINTA    L+  VV LE+PS +NGVGAN EFE+SQ 
Sbjct: 421 NSCPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSEFESSQE 480

Query: 481 EQQSNIDSAVNNSNNSGRHGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGRFTD 540
           E   NIDSAV +SNNSGR GGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGG+ TD
Sbjct: 481 EDYRNIDSAVISSNNSGR-GGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGQLTD 540

Query: 541 RRQLDAHIADASNQRENRTRRWW 559
            RQLDA  A++SNQRENRTRRWW
Sbjct: 541 GRQLDARSAESSNQRENRTRRWW 556

[Close]

BLAST of Tan0012556 vs. NCBI nr
Match: XP_022950071.1 (uncharacterized protein LOC111453262 isoform X2 [Cucurbita moschata])

HSP 1 Score: 859.4 bits (2219), Expect = 1.8e-245
Identity = 445/564 (78.90%), Postives = 481/564 (85.28%), Query Frame = 0


Query: 1   MNIDSQRILEEFPQSTAR-VMDSNQRVDEHGPIQPPTCACCQRVLVPDNDATVELQDIGI 60
           M+IDSQRILEEFP STAR +MD+NQ+V+E GP+QP TCA C+ VL PDNDAT E + IGI
Sbjct: 1   MDIDSQRILEEFPHSTARIIMDNNQQVNELGPVQPLTCAFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRR-RHGSSESGENLFSQEFTHMINLVRQSQSSI 120
           CGDCKFLL EDIETP+RDS HR TPRGRR RHGSSES ENLFSQEFT MINLVRQ QSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SGHGDRYVDGSNAADVMQHSSSHTTPNQSRRWRQVYSDSENDGLDSIDSMFGESESNFSF 180
           SGH DRY DGSNAAD MQHSSSHTTPNQSRRWRQVYSDSENDGLDS+DSMFGESESNFSF
Sbjct: 121 SGHDDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASMDGHSFLDTDIFVMPDDESD-DTDTDIDPMHAGVG 240
           GPYRHSYGDSD VSYRTYGGDSDAS+DGHSFLDTD FV+PD+ S  DTDTDIDPM AGV 
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGSGVDTDTDIDPMRAGVV 240

Query: 241 HWDSSDEEDDLGELTEADTEQDALEVESSEATPQLQNFHVSSATGRGNSGNWHEQLSSPE 300
           HWDSSDEEDD GELTEADTE DA           LQ+FHVSSATGRGN GNW+E+LSS E
Sbjct: 241 HWDSSDEEDDRGELTEADTEHDA-----------LQDFHVSSATGRGNYGNWNEELSSSE 300

Query: 301 YEPG---QIRRNRRFYSITDYARSELLSFVGDSGDYLDQQGFEELLEQIAETTSARRGAP 360
           YE     QIRRN RFYSIT+YA SE +S+VGDSGDYLD+Q FEELLEQIAETTS+RRGAP
Sbjct: 301 YEGSAREQIRRNMRFYSITNYAHSESVSYVGDSGDYLDRQSFEELLEQIAETTSSRRGAP 360

Query: 361 PAAVSSVKNLPRLVVTNEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPFCILPWLSA 420
           PAA+S VKNL RLV++ EHLKHD+ISCAICKDF LVGVEVNQLPCLHLYHP CILPWL+A
Sbjct: 361 PAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCLHLYHPPCILPWLTA 420

Query: 421 RNSCPLCRYELPTDDRDYEEGKRSSINTAEIQGLRHRVVDLESPSNINGVGANQEFETSQ 480
           RNSCPLCRYELPTDDRDYEEGK+SS N AEIQ L+H+V  LES S++N VGAN EF+TSQ
Sbjct: 421 RNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSDLNEVGANPEFDTSQ 480

Query: 481 GEQQSNIDSAVNNSNNSGRHGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGRFT 540
           GE+ SNID AV+NS NSGR  GRR WLFVAAP+VGLVGIALM W GNP       GGR T
Sbjct: 481 GEELSNIDPAVSNSYNSGR--GRRSWLFVAAPVVGLVGIALMFWVGNP------SGGRIT 540

Query: 541 DRRQLDAHIADASNQRENRTRRWW 559
           DRRQLD   AD+SNQRENRTRRWW
Sbjct: 541 DRRQLDTRGADSSNQRENRTRRWW 545

[Close]

BLAST of Tan0012556 vs. NCBI nr
Match: XP_023543725.1 (E3 ubiquitin-protein ligase Praja-2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 858.2 bits (2216), Expect = 3.9e-245
Identity = 447/589 (75.89%), Postives = 485/589 (82.34%), Query Frame = 0


Query: 1   MNIDSQRILEEFPQSTAR-VMDSNQRVDEHGPIQPPTCACCQRVLVPDNDATVELQDIGI 60
           M+IDSQRILEEFPQSTA  +MD+NQ+V+E GP+QP TCA C+ VL P+NDAT E + IGI
Sbjct: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRR-RHGSSESGENLFSQEFTHMINLVRQSQSSI 120
           CGDCKFLL EDIETP+RDS HR TPRGRR RHGSSES ENLFSQEFT MINLVRQ QSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SGHGDRYVDGSNAADVMQHSSSHTTPNQSRRWRQVYSDSENDGLDSIDSMFGESESNFSF 180
           SGHGDRY DGSNAAD MQHSSSHTTPNQSRRWRQVYSDSENDGLDS+DSMFGESESNFSF
Sbjct: 121 SGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASMDGHSFLDTDIFVMPDDESD-DTDTDIDPMHAGVG 240
           GPYRHSYGDSD VSYRTYGGDSDAS+DGHSFLDTD FV+PD+  D DTDTDIDPM AGV 
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGVV 240

Query: 241 HWDSSDEEDDLGELTEADTEQDALEVESSEATPQLQNFHVSSATGRGNSGNWHEQLSSPE 300
           HWDSSDEEDD GELTEADTE DA           LQ+FHVSSATGRGN GNW+E+LSS E
Sbjct: 241 HWDSSDEEDDRGELTEADTEHDA-----------LQDFHVSSATGRGNYGNWNEELSSSE 300

Query: 301 YE----------------------------PGQIRRNRRFYSITDYARSELLSFVGDSGD 360
           YE                            PGQIRRN RFYSIT+YA+SE +S+VGDSGD
Sbjct: 301 YEGSARDSITNNARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGD 360

Query: 361 YLDQQGFEELLEQIAETTSARRGAPPAAVSSVKNLPRLVVTNEHLKHDNISCAICKDFLL 420
           YLD+Q FEELLEQIAETTS+RRGAPPAA+S VKNL RLV++ EHLKHD+ISCAICKDF L
Sbjct: 361 YLDRQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFL 420

Query: 421 VGVEVNQLPCLHLYHPFCILPWLSARNSCPLCRYELPTDDRDYEEGKRSSINTAEIQGLR 480
           VGVEVNQLPCLHLYHP CILPWL+ARNSCPLCRYELPTDDRDYEEGK+SS N AEIQ L+
Sbjct: 421 VGVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQ 480

Query: 481 HRVVDLESPSNINGVGANQEFETSQGEQQSNIDSAVNNSNNSGRHGGRRRWLFVAAPIVG 540
           H+V  LES S++NGVGAN EF+TSQGE+ SNID AV+NS NSGR  GRR WLFVAAP+VG
Sbjct: 481 HQVTRLESRSDLNGVGANPEFDTSQGEELSNIDPAVSNSYNSGR--GRRSWLFVAAPVVG 540

Query: 541 LVGIALMLWFGNPLCDQRVPGGRFTDRRQLDAHIADASNQRENRTRRWW 559
           LVGIALM W GNP       GGR TDRRQLD   AD+SNQRE RTRRWW
Sbjct: 541 LVGIALMFWVGNP------SGGRITDRRQLDTRGADSSNQREKRTRRWW 570

[Close]

BLAST of Tan0012556 vs. ExPASy TrEMBL
Match: A0A0A0L2Z4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G337330 PE=4 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.7e-257
Identity = 463/568 (81.51%), Postives = 496/568 (87.32%), Query Frame = 0


Query: 1   MNIDSQRILEEFPQSTARV-MDSNQRVDEHGPIQPPTCACCQRVLVPDNDATVELQDIGI 60
           M+ID QRILEEFPQS   V M++NQRVDEH PIQ PTCA CQRVLVPD+DA  E + I I
Sbjct: 1   MDIDLQRILEEFPQSNVHVIMNNNQRVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRR-RHGSSESGENLFSQEFTHMINLVRQSQSSI 120
           CGDCKFLLLEDIETPVRDSYHRMTPRGRR    SSES ENLFSQEFTHMIN+VRQ QSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120

Query: 121 SGHGDRYVDGSNAADVMQHSSSHTTPNQSRRWRQVYSDSENDGLDSIDSMFGESESNFSF 180
           S HGDRY DGSNA D MQHSSSHTTPNQSRRWRQVYSDSENDGLDS DSMF ESESNFSF
Sbjct: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASM-DGHSFLDTDIFVMPDDESDDTDTDIDPMHAGVG 240
           GPYRHSYGDSD VS RTYGG+SD S+ DGHSFLDTD+F++P  E  DTDTDIDPM AGVG
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGESDVSLDDGHSFLDTDVFILP-GEGSDTDTDIDPMRAGVG 240

Query: 241 HWDSSDEEDDLGELTEADTEQDALEVESSEATPQLQNFHVSSATGRGNSGNWHEQLSSPE 300
           HWDSSDEEDD GE TEA+TEQ   EV SSEA PQLQNFHVSSA+ RGNS NW+EQLSSPE
Sbjct: 241 HWDSSDEEDDHGEFTEAETEQ---EVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPE 300

Query: 301 YE---PGQIRRNRRFYSITDYARSELLSFVGDSGDYLDQQGFEELLEQIAE-TTSARRGA 360
           YE   PG IRRNRRFYSIT+YA+SELLS+VGDSGDYLD+QGFEELLEQIAE TTS+RRGA
Sbjct: 301 YEGSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGA 360

Query: 361 PPAAVSSVKNLPRLVVTNEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPFCILPWLS 420
           PPAAVS VKNLPRLV++ EHLKHD+ISCAICKDFL +GVEVNQLPCLHLYHP CILPWLS
Sbjct: 361 PPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLS 420

Query: 421 ARNSCPLCRYELPTDDRDYEEGKRSSINTAEIQGLRHRVVDLESPSNINGVGANQEFETS 480
           ARNSCPLCRYELPTDDRDYEE K+SSIN A + GL+ RVV LE+PS +NGVGAN EFETS
Sbjct: 421 ARNSCPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETS 480

Query: 481 QGEQQSNIDSAVNNSNNSGRHGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGRF 540
           Q E Q NIDSAV +SNNSGR G RRRWLFVAAPIVGLVGIALMLWFGNPLCDQRV GG+ 
Sbjct: 481 QEEDQRNIDSAVISSNNSGR-GARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQL 540

Query: 541 TDRRQLDAHIADASNQRENRTRRWWSFV 562
           TD RQLDAH A++SNQRENR+RRWW  V
Sbjct: 541 TDGRQLDAHSAESSNQRENRSRRWWQIV 563

[Close]

BLAST of Tan0012556 vs. ExPASy TrEMBL
Match: A0A5D3C795 (E3 ubiquitin-protein ligase Praja-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold287G00690 PE=4 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.4e-256
Identity = 463/563 (82.24%), Postives = 493/563 (87.57%), Query Frame = 0


Query: 1   MNIDSQRILEEFPQSTARV-MDSNQRVDEHGPIQPPTCACCQRVLVPDNDATVELQDIGI 60
           M+ID QRILEEFPQS   V M+SNQRVDEH PIQ PTCA CQRVL  D+DAT E + I I
Sbjct: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRR-RHGSSESGENLFSQEFTHMINLVRQSQSSI 120
           CGDCKFLLLEDIETPVRDSYHRMTPRGRR    SSES ENLFSQEFTHMINLVRQ QSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSI 120

Query: 121 SGHGDRYVDGSNAADVMQHSSSHTTPNQSRRWRQVYSDSENDGLDSIDSMFGESESNFSF 180
           S HGDRY DGSNA D MQHSSSHTTPNQSRRWRQVYSDSENDGLDS DSMFGESESNFSF
Sbjct: 121 SSHGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASMDGHSFLDTDIFVMPDDESDDTDTDIDPMHAGVGH 240
           GPYRHSYGDSD VS RTYGGDSDAS+DGHSFLDTDIF++P  E  DTDTDIDPM AG+G+
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILP-GEGSDTDTDIDPMRAGIGN 240

Query: 241 WDSSDEEDDLGELTEADTEQDALEVESSEATPQLQNFHVSSATGRGNSGNWHEQLSSPEY 300
           WDSSDEEDD GE TEA+TEQ   E+ SSEA PQLQN +VSSA+ RGNS NW EQLSSPEY
Sbjct: 241 WDSSDEEDDHGEFTEAETEQ---EIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEY 300

Query: 301 E---PGQIRRNRRFYSITDYARSELLSFVGDSGDYLDQQGFEELLEQIAETTSARRGAPP 360
           E   PG IRRNRRFYSIT+YA+SELLS+VGDSGDYLD+QGFE+LLEQIAETTS+RRGAPP
Sbjct: 301 EGSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAETTSSRRGAPP 360

Query: 361 AAVSSVKNLPRLVVTNEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPFCILPWLSAR 420
           AAVS VKNLPRLV++ EHLKHD+ISCAICKDFLLVGVEVNQLPCLHLYHP CILPWLSAR
Sbjct: 361 AAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSAR 420

Query: 421 NSCPLCRYELPTDDRDYEEGKRSSINTAEIQGLRHRVVDLESPSNINGVGANQEFETSQG 480
           NSCPLCRYELPTDDRDYEE KRSSINTA    L+  VV LE+PS +NGVGAN EFE+SQ 
Sbjct: 421 NSCPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSEFESSQE 480

Query: 481 EQQSNIDSAVNNSNNSGRHGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGRFTD 540
           E   NIDSAV +SNNSGR GGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGG+ TD
Sbjct: 481 EDYRNIDSAVISSNNSGR-GGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGQLTD 540

Query: 541 RRQLDAHIADASNQRENRTRRWW 559
            RQLDA  A++SNQRENRTRRWW
Sbjct: 541 GRQLDARSAESSNQRENRTRRWW 556

[Close]

BLAST of Tan0012556 vs. ExPASy TrEMBL
Match: A0A1S3CIY7 (uncharacterized protein LOC103501491 OS=Cucumis melo OX=3656 GN=LOC103501491 PE=4 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.4e-256
Identity = 463/563 (82.24%), Postives = 493/563 (87.57%), Query Frame = 0


Query: 1   MNIDSQRILEEFPQSTARV-MDSNQRVDEHGPIQPPTCACCQRVLVPDNDATVELQDIGI 60
           M+ID QRILEEFPQS   V M+SNQRVDEH PIQ PTCA CQRVL  D+DAT E + I I
Sbjct: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRR-RHGSSESGENLFSQEFTHMINLVRQSQSSI 120
           CGDCKFLLLEDIETPVRDSYHRMTPRGRR    SSES ENLFSQEFTHMINLVRQ QSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSI 120

Query: 121 SGHGDRYVDGSNAADVMQHSSSHTTPNQSRRWRQVYSDSENDGLDSIDSMFGESESNFSF 180
           S HGDRY DGSNA D MQHSSSHTTPNQSRRWRQVYSDSENDGLDS DSMFGESESNFSF
Sbjct: 121 SSHGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASMDGHSFLDTDIFVMPDDESDDTDTDIDPMHAGVGH 240
           GPYRHSYGDSD VS RTYGGDSDAS+DGHSFLDTDIF++P  E  DTDTDIDPM AG+G+
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILP-GEGSDTDTDIDPMRAGIGN 240

Query: 241 WDSSDEEDDLGELTEADTEQDALEVESSEATPQLQNFHVSSATGRGNSGNWHEQLSSPEY 300
           WDSSDEEDD GE TEA+TEQ   E+ SSEA PQLQN +VSSA+ RGNS NW EQLSSPEY
Sbjct: 241 WDSSDEEDDHGEFTEAETEQ---EIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEY 300

Query: 301 E---PGQIRRNRRFYSITDYARSELLSFVGDSGDYLDQQGFEELLEQIAETTSARRGAPP 360
           E   PG IRRNRRFYSIT+YA+SELLS+VGDSGDYLD+QGFE+LLEQIAETTS+RRGAPP
Sbjct: 301 EGSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAETTSSRRGAPP 360

Query: 361 AAVSSVKNLPRLVVTNEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPFCILPWLSAR 420
           AAVS VKNLPRLV++ EHLKHD+ISCAICKDFLLVGVEVNQLPCLHLYHP CILPWLSAR
Sbjct: 361 AAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSAR 420

Query: 421 NSCPLCRYELPTDDRDYEEGKRSSINTAEIQGLRHRVVDLESPSNINGVGANQEFETSQG 480
           NSCPLCRYELPTDDRDYEE KRSSINTA    L+  VV LE+PS +NGVGAN EFE+SQ 
Sbjct: 421 NSCPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSEFESSQE 480

Query: 481 EQQSNIDSAVNNSNNSGRHGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGRFTD 540
           E   NIDSAV +SNNSGR GGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGG+ TD
Sbjct: 481 EDYRNIDSAVISSNNSGR-GGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGQLTD 540

Query: 541 RRQLDAHIADASNQRENRTRRWW 559
            RQLDA  A++SNQRENRTRRWW
Sbjct: 541 GRQLDARSAESSNQRENRTRRWW 556

[Close]

BLAST of Tan0012556 vs. ExPASy TrEMBL
Match: A0A6J1GDT0 (uncharacterized protein LOC111453262 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453262 PE=4 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 8.5e-246
Identity = 445/564 (78.90%), Postives = 481/564 (85.28%), Query Frame = 0


Query: 1   MNIDSQRILEEFPQSTAR-VMDSNQRVDEHGPIQPPTCACCQRVLVPDNDATVELQDIGI 60
           M+IDSQRILEEFP STAR +MD+NQ+V+E GP+QP TCA C+ VL PDNDAT E + IGI
Sbjct: 1   MDIDSQRILEEFPHSTARIIMDNNQQVNELGPVQPLTCAFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRR-RHGSSESGENLFSQEFTHMINLVRQSQSSI 120
           CGDCKFLL EDIETP+RDS HR TPRGRR RHGSSES ENLFSQEFT MINLVRQ QSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SGHGDRYVDGSNAADVMQHSSSHTTPNQSRRWRQVYSDSENDGLDSIDSMFGESESNFSF 180
           SGH DRY DGSNAAD MQHSSSHTTPNQSRRWRQVYSDSENDGLDS+DSMFGESESNFSF
Sbjct: 121 SGHDDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASMDGHSFLDTDIFVMPDDESD-DTDTDIDPMHAGVG 240
           GPYRHSYGDSD VSYRTYGGDSDAS+DGHSFLDTD FV+PD+ S  DTDTDIDPM AGV 
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGSGVDTDTDIDPMRAGVV 240

Query: 241 HWDSSDEEDDLGELTEADTEQDALEVESSEATPQLQNFHVSSATGRGNSGNWHEQLSSPE 300
           HWDSSDEEDD GELTEADTE DA           LQ+FHVSSATGRGN GNW+E+LSS E
Sbjct: 241 HWDSSDEEDDRGELTEADTEHDA-----------LQDFHVSSATGRGNYGNWNEELSSSE 300

Query: 301 YEPG---QIRRNRRFYSITDYARSELLSFVGDSGDYLDQQGFEELLEQIAETTSARRGAP 360
           YE     QIRRN RFYSIT+YA SE +S+VGDSGDYLD+Q FEELLEQIAETTS+RRGAP
Sbjct: 301 YEGSAREQIRRNMRFYSITNYAHSESVSYVGDSGDYLDRQSFEELLEQIAETTSSRRGAP 360

Query: 361 PAAVSSVKNLPRLVVTNEHLKHDNISCAICKDFLLVGVEVNQLPCLHLYHPFCILPWLSA 420
           PAA+S VKNL RLV++ EHLKHD+ISCAICKDF LVGVEVNQLPCLHLYHP CILPWL+A
Sbjct: 361 PAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCLHLYHPPCILPWLTA 420

Query: 421 RNSCPLCRYELPTDDRDYEEGKRSSINTAEIQGLRHRVVDLESPSNINGVGANQEFETSQ 480
           RNSCPLCRYELPTDDRDYEEGK+SS N AEIQ L+H+V  LES S++N VGAN EF+TSQ
Sbjct: 421 RNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSDLNEVGANPEFDTSQ 480

Query: 481 GEQQSNIDSAVNNSNNSGRHGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGRFT 540
           GE+ SNID AV+NS NSGR  GRR WLFVAAP+VGLVGIALM W GNP       GGR T
Sbjct: 481 GEELSNIDPAVSNSYNSGR--GRRSWLFVAAPVVGLVGIALMFWVGNP------SGGRIT 540

Query: 541 DRRQLDAHIADASNQRENRTRRWW 559
           DRRQLD   AD+SNQRENRTRRWW
Sbjct: 541 DRRQLDTRGADSSNQRENRTRRWW 545

[Close]

BLAST of Tan0012556 vs. ExPASy TrEMBL
Match: A0A6J1GEL6 (E3 ubiquitin-protein ligase Praja-2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453262 PE=4 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.2e-244
Identity = 447/589 (75.89%), Postives = 483/589 (82.00%), Query Frame = 0


Query: 1   MNIDSQRILEEFPQSTAR-VMDSNQRVDEHGPIQPPTCACCQRVLVPDNDATVELQDIGI 60
           M+IDSQRILEEFP STAR +MD+NQ+V+E GP+QP TCA C+ VL PDNDAT E + IGI
Sbjct: 1   MDIDSQRILEEFPHSTARIIMDNNQQVNELGPVQPLTCAFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRR-RHGSSESGENLFSQEFTHMINLVRQSQSSI 120
           CGDCKFLL EDIETP+RDS HR TPRGRR RHGSSES ENLFSQEFT MINLVRQ QSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SGHGDRYVDGSNAADVMQHSSSHTTPNQSRRWRQVYSDSENDGLDSIDSMFGESESNFSF 180
           SGH DRY DGSNAAD MQHSSSHTTPNQSRRWRQVYSDSENDGLDS+DSMFGESESNFSF
Sbjct: 121 SGHDDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASMDGHSFLDTDIFVMPDDESD-DTDTDIDPMHAGVG 240
           GPYRHSYGDSD VSYRTYGGDSDAS+DGHSFLDTD FV+PD+ S  DTDTDIDPM AGV 
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGSGVDTDTDIDPMRAGVV 240

Query: 241 HWDSSDEEDDLGELTEADTEQDALEVESSEATPQLQNFHVSSATGRGNSGNWHEQLSSPE 300
           HWDSSDEEDD GELTEADTE DA           LQ+FHVSSATGRGN GNW+E+LSS E
Sbjct: 241 HWDSSDEEDDRGELTEADTEHDA-----------LQDFHVSSATGRGNYGNWNEELSSSE 300

Query: 301 YE----------------------------PGQIRRNRRFYSITDYARSELLSFVGDSGD 360
           YE                            PGQIRRN RFYSIT+YA SE +S+VGDSGD
Sbjct: 301 YEGSARDSITNNAQSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAHSESVSYVGDSGD 360

Query: 361 YLDQQGFEELLEQIAETTSARRGAPPAAVSSVKNLPRLVVTNEHLKHDNISCAICKDFLL 420
           YLD+Q FEELLEQIAETTS+RRGAPPAA+S VKNL RLV++ EHLKHD+ISCAICKDF L
Sbjct: 361 YLDRQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFL 420

Query: 421 VGVEVNQLPCLHLYHPFCILPWLSARNSCPLCRYELPTDDRDYEEGKRSSINTAEIQGLR 480
           VGVEVNQLPCLHLYHP CILPWL+ARNSCPLCRYELPTDDRDYEEGK+SS N AEIQ L+
Sbjct: 421 VGVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQ 480

Query: 481 HRVVDLESPSNINGVGANQEFETSQGEQQSNIDSAVNNSNNSGRHGGRRRWLFVAAPIVG 540
           H+V  LES S++N VGAN EF+TSQGE+ SNID AV+NS NSGR  GRR WLFVAAP+VG
Sbjct: 481 HQVTRLESRSDLNEVGANPEFDTSQGEELSNIDPAVSNSYNSGR--GRRSWLFVAAPVVG 540

Query: 541 LVGIALMLWFGNPLCDQRVPGGRFTDRRQLDAHIADASNQRENRTRRWW 559
           LVGIALM W GNP       GGR TDRRQLD   AD+SNQRENRTRRWW
Sbjct: 541 LVGIALMFWVGNP------SGGRITDRRQLDTRGADSSNQRENRTRRWW 570

[Close]

BLAST of Tan0012556 vs. TAIR 10
Match: AT5G01980.1 (RING/U-box superfamily protein )

HSP 1 Score: 341.3 bits (874), Expect = 1.5e-93
Identity = 230/547 (42.05%), Postives = 306/547 (55.94%), Query Frame = 0


Query: 32  IQPPTCACCQRVLVPDNDATVELQDIGICGDCKFLLLEDIETPVRDSYHRMTPRGRR--- 91
           I    C  C RV+     A+ +L+   +C DCKFLLLED  TP      R T R RR   
Sbjct: 19  IPSSACNLCGRVV---QSASDDLEIFSLCVDCKFLLLEDFGTPAPPLARRQTIRRRRMRR 78

Query: 92  -----RHGSSES--GENLFSQEFTHMINLVRQSQSSISGHGDRYVDGSNAADVMQHSSSH 151
                RH SSES    +L SQ+FTH+I++ RQS S++    D   D          +SSH
Sbjct: 79  RRRTTRHDSSESVGVGDLSSQQFTHLISIARQSLSTVQASEDLRDD--------LRTSSH 138

Query: 152 TTPNQSRRWRQVYSDSENDGLDSIDSMFGESESNFSFGPYRHSYGDSDAVSYRTYGGDSD 211
           TTP+ S RWR ++S+SE+D  D+    FGE+ESN SF  YR  + D+D +S+  YGG+SD
Sbjct: 139 TTPSGSTRWR-LFSESESDDFDN----FGETESNASFSLYRFPHADNDTISFSAYGGESD 198

Query: 212 ASMDGHSFLDTDIFVMPDDESD-DTDTDIDPMHAGVGHWDSSDEEDDLGELTEADTEQDA 271
           AS D H+    DIFV PDD SD D DTDIDPM AG+  W+S +E          D E + 
Sbjct: 199 ASTDRHA----DIFVQPDDRSDIDFDTDIDPMRAGLNQWNSDEE----------DREWEE 258

Query: 272 LEVESSEATPQLQNFHVSSATGRGNSGNWHEQLSSPEYEPG---QIRRNRRFYSITDYAR 331
               S  A  + +N+  S +     S +   +  SPE E     +I   R   S   +  
Sbjct: 259 GAGPSGVAGTRYRNYLASPS----ESYSSMTRFDSPELERSFRQRIIERRHSLSRNIFTG 318

Query: 332 SELLSF---VGDSGDYLDQQGFEELLEQIAETTSARRGAPPAAVSSVKNLPRLVVTNEHL 391
            E L F     + GDYLD++GF+ELLEQ+AE+ ++RRGAPPA+VS V+NLPR+++  EH+
Sbjct: 319 LEDLDFSPYAANVGDYLDERGFDELLEQLAESDNSRRGAPPASVSCVRNLPRVIIAEEHV 378

Query: 392 KHDNISCAICKDFLLVGVEVNQLPCLHLYHPFCILPWLSARNSCPLCRYELPTDDRDYEE 451
               + CAICK+   +  E  QLPCLHLYH  CI+PWLSARNSCPLCRYELPTDD+DYEE
Sbjct: 379 M-KGLVCAICKELFSLRNETTQLPCLHLYHAHCIVPWLSARNSCPLCRYELPTDDKDYEE 438

Query: 452 GKRSSINTAEIQGLRHRVVDLESPSNINGVGANQEFETSQGEQQSNIDSAVNNSNNSGRH 511
           GKR+ ++ +E           +S S+ +G  + +E    +GE +S +             
Sbjct: 439 GKRNVLDVSE-----------DSSSSDDGTESGEEEYVERGESESGVSRV---------- 492

Query: 512 GGRRRWLFV-AAPIVGLVGIALMLWFGNPLCDQRVPGGRFTDRRQLDAHIADASNQRENR 561
             R RWLF+ AAP+V LVG+ L +W  +P             RR     IA + +QRENR
Sbjct: 499 -SRGRWLFLAAAPVVSLVGVVLAMWLSSP------------HRR----GIAISHSQRENR 492

[Close]

BLAST of Tan0012556 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 125.6 bits (314), Expect = 1.3e-28
Identity = 56/123 (45.53%), Postives = 78/123 (63.41%), Query Frame = 0


Query: 325 GDSGDYLDQQGFEELLEQIAETTSARRGAPPAAVSSVKNLPRLVVTNEHLKHDNISCAIC 384
           G+ GDY    G E+L++Q+AE    R G PPA+ S++  LP + VT + LK +   CA+C
Sbjct: 160 GNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVC 219

Query: 385 KDFLLVGVEVNQLPCLHLYHPFCILPWLSARNSCPLCRYELPTDDRDYEEGKRSSINTAE 444
            D    G +V Q+PC H++H  C+LPWL   NSCP+CR+ELPTDD DYE   + S  + +
Sbjct: 220 MDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGD 279

Query: 445 IQG 448
            QG
Sbjct: 280 GQG 282

[Close]

BLAST of Tan0012556 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 111.7 bits (278), Expect = 1.9e-24
Identity = 55/139 (39.57%), Postives = 76/139 (54.68%), Query Frame = 0


Query: 325 GDSGDYLDQQGFEELLEQIAETTSARRGAPPAAVSSVKNLPRLVVTNEHLKHDNISCAIC 384
           G++GDY    G EEL EQ++  T+ RRG PPA  S++  LP + +   HL+  + +C +C
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTT-RRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 385 KDFLLVGVEVNQLPCLHLYHPFCILPWLSARNSCPLCRYELP------------TDDRDY 444
           KD   +G E  Q+PC H+YH  CI+PWL   NSCP+CR ELP            T  R+Y
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNY 254

Query: 445 EEGKRSSINTAEIQGLRHR 452
                SS + +   G   R
Sbjct: 255 RSSSSSSSSNSRENGNERR 272

[Close]

BLAST of Tan0012556 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 111.7 bits (278), Expect = 1.9e-24
Identity = 55/139 (39.57%), Postives = 76/139 (54.68%), Query Frame = 0


Query: 325 GDSGDYLDQQGFEELLEQIAETTSARRGAPPAAVSSVKNLPRLVVTNEHLKHDNISCAIC 384
           G++GDY    G EEL EQ++  T+ RRG PPA  S++  LP + +   HL+  + +C +C
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTT-RRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 385 KDFLLVGVEVNQLPCLHLYHPFCILPWLSARNSCPLCRYELP------------TDDRDY 444
           KD   +G E  Q+PC H+YH  CI+PWL   NSCP+CR ELP            T  R+Y
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNY 254

Query: 445 EEGKRSSINTAEIQGLRHR 452
                SS + +   G   R
Sbjct: 255 RSSSSSSSSNSRENGNERR 272

[Close]

BLAST of Tan0012556 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 111.7 bits (278), Expect = 1.9e-24
Identity = 55/139 (39.57%), Postives = 76/139 (54.68%), Query Frame = 0


Query: 325 GDSGDYLDQQGFEELLEQIAETTSARRGAPPAAVSSVKNLPRLVVTNEHLKHDNISCAIC 384
           G++GDY    G EEL EQ++  T+ RRG PPA  S++  LP + +   HL+  + +C +C
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTT-RRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 385 KDFLLVGVEVNQLPCLHLYHPFCILPWLSARNSCPLCRYELP------------TDDRDY 444
           KD   +G E  Q+PC H+YH  CI+PWL   NSCP+CR ELP            T  R+Y
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNY 254

Query: 445 EEGKRSSINTAEIQGLRHR 452
                SS + +   G   R
Sbjct: 255 RSSSSSSSSNSRENGNERR 272

The following BLAST results are available for this feature:

BLAST of Tan0012556 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPN7	1.8e-27	45.53	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	1.2e-26	46.55	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
O22197	2.7e-23	39.57	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q9SPL2	5.1e-22	33.62	E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV...	[more]
Q94AK4	8.8e-22	47.06	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]

BLAST of Tan0012556 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038882118.1	1.1e-268	85.11	E3 ubiquitin-protein ligase Praja-2 [Benincasa hispida] >XP_038882119.1 E3 ubiqu...	[more]
XP_004142724.1	7.6e-257	81.51	E3 ubiquitin-protein ligase Praja-2 [Cucumis sativus] >KGN54476.1 hypothetical p...	[more]
XP_008463301.1	2.9e-256	82.24	PREDICTED: uncharacterized protein LOC103501491 [Cucumis melo] >TYK06209.1 E3 ub...	[more]
XP_022950071.1	1.8e-245	78.90	uncharacterized protein LOC111453262 isoform X2 [Cucurbita moschata]	[more]
XP_023543725.1	3.9e-245	75.89	E3 ubiquitin-protein ligase Praja-2 isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Tan0012556 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2Z4	3.7e-257	81.51	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G337330 P...	[more]
A0A5D3C795	1.4e-256	82.24	E3 ubiquitin-protein ligase Praja-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3CIY7	1.4e-256	82.24	uncharacterized protein LOC103501491 OS=Cucumis melo OX=3656 GN=LOC103501491 PE=...	[more]
A0A6J1GDT0	8.5e-246	78.90	uncharacterized protein LOC111453262 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1GEL6	1.2e-244	75.89	E3 ubiquitin-protein ligase Praja-2-like isoform X1 OS=Cucurbita moschata OX=366...	[more]

BLAST of Tan0012556 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01980.1	1.5e-93	42.05	RING/U-box superfamily protein	[more]
AT3G19950.1	1.3e-28	45.53	RING/U-box superfamily protein	[more]
AT2G40830.2	1.9e-24	39.57	RING-H2 finger C1A	[more]
AT2G40830.1	1.9e-24	39.57	RING-H2 finger C1A	[more]
AT2G40830.3	1.9e-24	39.57	RING-H2 finger C1A	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 381..421 e-value: 2.2E-5 score: 33.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 381..422 score: 11.441815
None	No IPR available	PFAM	PF13923	zf-C3HC4_2	coord: 381..421 e-value: 2.6E-8 score: 33.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 220..249
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 277..300
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 277..295
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 464..494
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 228..242
None	No IPR available	PANTHER	PTHR15710:SF41	OS06G0101300 PROTEIN	coord: 323..508
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 323..508
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 368..428
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 342..428 e-value: 7.4E-17 score: 62.8

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0012556.1	Tan0012556.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity