Tan0012457 (gene) Snake gourd v1

Overview


Name	Tan0012457
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	RNase H domain-containing protein
Location	LG10: 2762049 .. 2762549 (+)
RNA-Seq Expression	Tan0012457
Synteny	Tan0012457

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATAAAGTCAATGTTGGTGGTATTGGTTGGGTTATCCGTGACTCTAATGGCTCTCTGATTTGTGCAGGAGGGAAGCAAATTAAAAGAAGTTGGCAAATTAAAGTGCTGGAAGCGAAGGCGATTCTTGAGGGTCTTGAAGCGTTTAACAAGTGTTATGTCGTCCATCACAAGTTGCTGATTGTGGAGTCGGACTCGAGCGAAGTGATCAAGTGCCTGAACGAGGATGTTGAAGACCTCTCTGAGTTATACAATTTTGTTAAAGCGATTAATTGTTTTGTTAGGCGCTTTCCTGTTGTTAATTTTGTTAAGTGTCATAGGTCTAAAAACCTCTTAGCTCATAACATTGCTAGAAGTGTTTGTAGTTTAGGTGATTTTGATGGAGCTTTTTGCTCCCCCCATGGCTCTAACGTTTCTAGGTGCGGTTTTGGCGAGTACCCATCTTGGGTCTCCGACCTGTTGCCTGCGGGCTGCACTCCTTGTGGGTCCGTTGTGGGCTAG

mRNA sequence

ATGAATAAAGTCAATGTTGGTGGTATTGGTTGGGTTATCCGTGACTCTAATGGCTCTCTGATTTGTGCAGGAGGGAAGCAAATTAAAAGAAGTTGGCAAATTAAAGTGCTGGAAGCGAAGGCGATTCTTGAGGGTCTTGAAGCGTTTAACAAGTGTTATGTCGTCCATCACAAGTTGCTGATTGTGGAGTCGGACTCGAGCGAAGTGATCAAGTGCCTGAACGAGGATGTTGAAGACCTCTCTGAGTTATACAATTTTGTTAAAGCGATTAATTGTTTTGTTAGGCGCTTTCCTGTTGTTAATTTTGTTAAGTGTCATAGGTCTAAAAACCTCTTAGCTCATAACATTGCTAGAAGTGTTTGTAGTTTAGGTGATTTTGATGGAGCTTTTTGCTCCCCCCATGGCTCTAACGTTTCTAGGTGCGGTTTTGGCGAGTACCCATCTTGGGTCTCCGACCTGTTGCCTGCGGGCTGCACTCCTTGTGGGTCCGTTGTGGGCTAG

Coding sequence (CDS)

ATGAATAAAGTCAATGTTGGTGGTATTGGTTGGGTTATCCGTGACTCTAATGGCTCTCTGATTTGTGCAGGAGGGAAGCAAATTAAAAGAAGTTGGCAAATTAAAGTGCTGGAAGCGAAGGCGATTCTTGAGGGTCTTGAAGCGTTTAACAAGTGTTATGTCGTCCATCACAAGTTGCTGATTGTGGAGTCGGACTCGAGCGAAGTGATCAAGTGCCTGAACGAGGATGTTGAAGACCTCTCTGAGTTATACAATTTTGTTAAAGCGATTAATTGTTTTGTTAGGCGCTTTCCTGTTGTTAATTTTGTTAAGTGTCATAGGTCTAAAAACCTCTTAGCTCATAACATTGCTAGAAGTGTTTGTAGTTTAGGTGATTTTGATGGAGCTTTTTGCTCCCCCCATGGCTCTAACGTTTCTAGGTGCGGTTTTGGCGAGTACCCATCTTGGGTCTCCGACCTGTTGCCTGCGGGCTGCACTCCTTGTGGGTCCGTTGTGGGCTAG

Protein sequence

MNKVNVGGIGWVIRDSNGSLICAGGKQIKRSWQIKVLEAKAILEGLEAFNKCYVVHHKLLIVESDSSEVIKCLNEDVEDLSELYNFVKAINCFVRRFPVVNFVKCHRSKNLLAHNIARSVCSLGDFDGAFCSPHGSNVSRCGFGEYPSWVSDLLPAGCTPCGSVVG

Homology

[Close]

BLAST of Tan0012457 vs. NCBI nr
Match: XP_021808158.1 (uncharacterized protein LOC110751913 [Prunus avium])

HSP 1 Score: 68.9 bits (167), Expect = 4.6e-08
Identity = 48/131 (36.64%), Postives = 63/131 (48.09%), Query Frame = 0


Query: 7   GGIGWVIRDSNGSLICAGGKQIKRSWQIKVLEAKAILEGLEAFNKCYVVHHKLLIVESDS 66
           GG+GWVIRDS+G L+CAGG+   R     ++E  AI   L A     + H   ++VESD+
Sbjct: 99  GGVGWVIRDSHGLLLCAGGEGDLRGSSALLMELLAIRHALSACAHFQLTH---VMVESDT 158

Query: 67  SEVIKCLNEDVEDLSELYNFVKAINCFVRRFPVVNFVKCHRSKNLLAHNIARSVCSLGDF 126
              I  LN      S+L   V  I   V  F  V+FV   +S N +AH +A         
Sbjct: 159 QRGISMLNGQATVDSDLEGIVFDIQQLVATFSQVSFVFAPKSCNRVAHTVA--------- 215

Query: 127 DGAFCSPHGSN 138
              F S HG N
Sbjct: 219 --GFVSKHGGN 215

[Close]

BLAST of Tan0012457 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 67.0 bits (162), Expect = 1.7e-07
Identity = 41/112 (36.61%), Postives = 63/112 (56.25%), Query Frame = 0


Query: 7   GGIGWVIRDSNGSLICAGGKQIKRSWQIKVLEAKAILEGLEAFNKCYVVHHKLLIVESDS 66
           GGIGW+IR  +G ++ AG + ++    +K+LEA AILEGL       V+  + L +E+DS
Sbjct: 94  GGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVL--RPLHIETDS 153

Query: 67  SEVIKCLNEDVEDLSELYNFVKAINCFVRRFPVVNFVKCHRSKNLLAHNIAR 119
           +EV   LN   EDL++    V+ I        ++ F K  R  N  AH++A+
Sbjct: 154 AEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLAQ 203

[Close]

BLAST of Tan0012457 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 65.9 bits (159), Expect = 3.9e-07
Identity = 39/114 (34.21%), Postives = 60/114 (52.63%), Query Frame = 0


Query: 5   NVGGIGWVIRDSNGSLICAGGKQIKRSWQIKVLEAKAILEGLEAFNKCYVVHHKLLIVES 64
           N GGIGW++RD  G +I A  + I+    I  LE  AI EGL A  +    H + + +ES
Sbjct: 100 NTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQ---EHCRPIHLES 159

Query: 65  DSSEVIKCLNEDVEDLSELYNFVKAINCFVRRFPVVNFVKCHRSKNLLAHNIAR 119
           DS E I  L+   +D +E+   ++ I   ++   +V+     R  N +AH +AR
Sbjct: 160 DSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVAHGLAR 210

[Close]

BLAST of Tan0012457 vs. NCBI nr
Match: XP_021841246.1 (uncharacterized protein LOC110781352 [Spinacia oleracea])

HSP 1 Score: 64.7 bits (156), Expect = 8.6e-07
Identity = 36/110 (32.73%), Postives = 61/110 (55.45%), Query Frame = 0


Query: 8   GIGWVIRDSNGSLICAGGKQIKRSWQIKVLEAKAILEGLEAFNKCYVVHHKLLIVESDSS 67
           G+G +IRD  G+++ A   Q+   W++ ++EAK+I+ GL+   +C     + ++VESD  
Sbjct: 99  GMGVIIRDEEGAVVRAACLQVAHQWEVSIVEAKSIVLGLKLAIQCGA---RDVVVESDCL 158

Query: 68  EVIKCLNEDVEDLSELYNFVKAINCFVRRFPVVNFVKCHRSKNLLAHNIA 118
           +VIK +N ++ D   L    + I      F  +NFV  +R  NL  H +A
Sbjct: 159 KVIKLVNGEIRDGLYLGMIGREIREIASLFDSINFVHVYREANLADHTMA 205

[Close]

BLAST of Tan0012457 vs. NCBI nr
Match: KNA03763.1 (hypothetical protein SOVF_206000, partial [Spinacia oleracea])

HSP 1 Score: 64.7 bits (156), Expect = 8.6e-07
Identity = 40/110 (36.36%), Postives = 58/110 (52.73%), Query Frame = 0


Query: 8   GIGWVIRDSNGSLICAGGKQIKRSWQIKVLEAKAILEGLEAFNKCYVVHHKLLIVESDSS 67
           G+G VI D  GS       Q++ SW +++ EAKAI+ GL+  N+C   H + +IVE D+S
Sbjct: 140 GLGVVISDYEGSARRVACSQVRHSWSVEITEAKAIVLGLQVANQC---HERNIIVECDNS 199

Query: 68  EVIKCLNEDVEDLSELYNFVKAINCFVRRFPVVNFVKCHRSKNLLAHNIA 118
            +IK L E+  D S L   ++ +      F  V F    R  N  AH +A
Sbjct: 200 LIIKKLKENKVDGSYLGVILREVKHLSSLFHSVVFSHVFREANEAAHTMA 246

[Close]

BLAST of Tan0012457 vs. ExPASy TrEMBL
Match: A0A6P5S2T0 (uncharacterized protein LOC110751913 OS=Prunus avium OX=42229 GN=LOC110751913 PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 2.2e-08
Identity = 48/131 (36.64%), Postives = 63/131 (48.09%), Query Frame = 0


Query: 7   GGIGWVIRDSNGSLICAGGKQIKRSWQIKVLEAKAILEGLEAFNKCYVVHHKLLIVESDS 66
           GG+GWVIRDS+G L+CAGG+   R     ++E  AI   L A     + H   ++VESD+
Sbjct: 99  GGVGWVIRDSHGLLLCAGGEGDLRGSSALLMELLAIRHALSACAHFQLTH---VMVESDT 158

Query: 67  SEVIKCLNEDVEDLSELYNFVKAINCFVRRFPVVNFVKCHRSKNLLAHNIARSVCSLGDF 126
              I  LN      S+L   V  I   V  F  V+FV   +S N +AH +A         
Sbjct: 159 QRGISMLNGQATVDSDLEGIVFDIQQLVATFSQVSFVFAPKSCNRVAHTVA--------- 215

Query: 127 DGAFCSPHGSN 138
              F S HG N
Sbjct: 219 --GFVSKHGGN 215

[Close]

BLAST of Tan0012457 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 8.4e-08
Identity = 41/112 (36.61%), Postives = 63/112 (56.25%), Query Frame = 0


Query: 7   GGIGWVIRDSNGSLICAGGKQIKRSWQIKVLEAKAILEGLEAFNKCYVVHHKLLIVESDS 66
           GGIGW+IR  +G ++ AG + ++    +K+LEA AILEGL       V+  + L +E+DS
Sbjct: 94  GGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVL--RPLHIETDS 153

Query: 67  SEVIKCLNEDVEDLSELYNFVKAINCFVRRFPVVNFVKCHRSKNLLAHNIAR 119
           +EV   LN   EDL++    V+ I        ++ F K  R  N  AH++A+
Sbjct: 154 AEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLAQ 203

[Close]

BLAST of Tan0012457 vs. ExPASy TrEMBL
Match: A0A803MUM1 (Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.9e-07
Identity = 39/109 (35.78%), Postives = 60/109 (55.05%), Query Frame = 0


Query: 9   IGWVIRDSNGSLICAGGKQIKRSWQIKVLEAKAILEGLEAFNKCYVVHHKLLIVESDSSE 68
           +G V+RD  G ++ AG +Q+ +SW   ++EAKA++ GL+   +C V   K +IVESD   
Sbjct: 186 MGVVVRDHLGEVVRAGCQQVHQSWDASIVEAKAMVLGLKLAMQCNV---KRVIVESDCER 245

Query: 69  VIKCLNEDVEDLSELYNFVKAINCFVRRFPVVNFVKCHRSKNLLAHNIA 118
           ++K +N   +D S L    K I C    F  + F    R  N +AH +A
Sbjct: 246 LVKLVNGVGKDGSFLGLLAKEIKCVAEFFDSIVFAHVFREANSVAHAMA 291

[Close]

BLAST of Tan0012457 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.9e-07
Identity = 39/114 (34.21%), Postives = 60/114 (52.63%), Query Frame = 0


Query: 5   NVGGIGWVIRDSNGSLICAGGKQIKRSWQIKVLEAKAILEGLEAFNKCYVVHHKLLIVES 64
           N GGIGW++RD  G +I A  + I+    I  LE  AI EGL A  +    H + + +ES
Sbjct: 100 NTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQ---EHCRPIHLES 159

Query: 65  DSSEVIKCLNEDVEDLSELYNFVKAINCFVRRFPVVNFVKCHRSKNLLAHNIAR 119
           DS E I  L+   +D +E+   ++ I   ++   +V+     R  N +AH +AR
Sbjct: 160 DSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVAHGLAR 210

[Close]

BLAST of Tan0012457 vs. ExPASy TrEMBL
Match: A0A0K9QAP7 (RNase H domain-containing protein (Fragment) OS=Spinacia oleracea OX=3562 GN=SOVF_206000 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 4.2e-07
Identity = 40/110 (36.36%), Postives = 58/110 (52.73%), Query Frame = 0


Query: 8   GIGWVIRDSNGSLICAGGKQIKRSWQIKVLEAKAILEGLEAFNKCYVVHHKLLIVESDSS 67
           G+G VI D  GS       Q++ SW +++ EAKAI+ GL+  N+C   H + +IVE D+S
Sbjct: 140 GLGVVISDYEGSARRVACSQVRHSWSVEITEAKAIVLGLQVANQC---HERNIIVECDNS 199

Query: 68  EVIKCLNEDVEDLSELYNFVKAINCFVRRFPVVNFVKCHRSKNLLAHNIA 118
            +IK L E+  D S L   ++ +      F  V F    R  N  AH +A
Sbjct: 200 LIIKKLKENKVDGSYLGVILREVKHLSSLFHSVVFSHVFREANEAAHTMA 246

[Close]

BLAST of Tan0012457 vs. TAIR 10
Match: AT1G27870.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 41.2 bits (95), Expect = 9.5e-04
Identity = 28/113 (24.78%), Postives = 61/113 (53.98%), Query Frame = 0


Query: 10  GWVIRDSNGSLICAG---GKQIKRSWQIKVLEAKAILEGLEAFNKCYVVHHKLLIVESDS 69
           GWV+RDSNGS + AG   G+++  + +    E +A++  ++    C+   +K +  E D+
Sbjct: 78  GWVVRDSNGSYLLAGQAIGRKVDNALE---SEIQALIISMQ---HCWSHGYKRVCFEGDN 137

Query: 70  SEVIKCLNEDVEDLSELYNFVKAINCFVRRFPVVNFVKCHRSKNLLAHNIARS 120
             +   +N   +    ++N+++ I+ ++R+F  ++F    R  N  A  +A++
Sbjct: 138 KMLFDLINGS-KVYFGVHNWIRDIHWWLRKFESIHFNWIRRHHNTPADILAKA 183

The following BLAST results are available for this feature:

BLAST of Tan0012457 vs.


Match Name	E-value	Identity	Description

BLAST of Tan0012457 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_021808158.1	4.6e-08	36.64	uncharacterized protein LOC110751913 [Prunus avium]	[more]
XP_022155262.1	1.7e-07	36.61	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022143535.1	3.9e-07	34.21	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_021841246.1	8.6e-07	32.73	uncharacterized protein LOC110781352 [Spinacia oleracea]	[more]
KNA03763.1	8.6e-07	36.36	hypothetical protein SOVF_206000, partial [Spinacia oleracea]	[more]

BLAST of Tan0012457 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6P5S2T0	2.2e-08	36.64	uncharacterized protein LOC110751913 OS=Prunus avium OX=42229 GN=LOC110751913 PE...	[more]
A0A6J1DNV9	8.4e-08	36.61	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A803MUM1	1.9e-07	35.78	Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1	[more]
A0A6J1CP26	1.9e-07	34.21	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A0K9QAP7	4.2e-07	36.36	RNase H domain-containing protein (Fragment) OS=Spinacia oleracea OX=3562 GN=SOV...	[more]

BLAST of Tan0012457 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G27870.1	9.5e-04	24.78	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..123 e-value: 6.2E-12 score: 47.6
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 5..119 e-value: 1.1E-19 score: 70.4
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 4..123
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 7..118 e-value: 7.6575E-18 score: 72.732
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 6..122

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0012457.1	Tan0012457.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity