Tan0012421 (gene) Snake gourd v1

Overview
NameTan0012421
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptiontRNA_int_end_N2 domain-containing protein
LocationLG10: 66656377 .. 66658422 (+)
RNA-Seq ExpressionTan0012421
SyntenyTan0012421
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGACATTTACTGAGCAAAACATGAGAGATGAAGAAGAATGTCTTTGTTCATTTTTAAATTTTTACATGCATGAAGTTGCAATTCAGTTGAGAATTTCAAGAATTTAGACTCACAGAATCCTTCAATAGGATTGAGCTATTTGGTTATTTTTATGTGTGTGTTTCAATATTTTTATTTGTCTCTAGGAAGCATGCTTCGACTACTCGATGGAATGATCAGATGGGAATGGCAGAAGTTGTAGAGAACAAGGCGACCTTTGAGCGACGGCGGCATTGTACGTTGTGGCAAGATTTATTGTTCCATTGAGGAAACTTTGTAGGTCAAACTATGATTTTATTATGGTTTTGACATCTCTATTGGTTTTGATATTGATTACAACATTTTTTGGGGAGTTATTCTCTTTCACATTGTCTTTAAGTGATTACTATAAGATAAGGTAATATCATGAAAAATAAAAGAAACTGGTAGTGTTTTGGAGAGTTTTTTTTTTTGTTGCTTTTTGGTTCCTTATTTAGAACATTACAATACAAATAAAGGGAAGAAATTCAAGCTTACAATGTACACGACGAAGCACTAGTAGTAGTCTTTATGTCTTGGTAAATAAATGAATTCATCATTTTTTAGTACTTTTTAACTAAAGTCCTTTTTCCTATCATTATCTCTATAGTAATGACATTTTATTGAGCTTATTTGTGTCCCACTATATAATTCATTTTATGTATCACTTATTCTTCTTTTCTATTTACAATATATATATACATCTATTGTTACTTGGGTAATGTTTATTTTAATGTGTCTCTTGTTTCAGAAAAGTTAGAAGTGTGTTGCCTTGAAGTGTTCCTTGTGCTTTCTAAATGTTGACTGTAGTATGAACCTTGGCTCAAAAATAGAGTTGTACCATTTAAGCAGAAATATGTAGGATCATGATCTCTTTTGAGACATTTAAATGACAATTTTTAAGCATGCTATCAAACATTCTTTTTTATGTTTTCAGATTTCTTATTGAAGTTGGGGCCTTGCATCTTCTTGATTATGATAATTTAAATCTTTCTTTGAAAGACGTATACAAGAAGGTAGCTGAAGGAAAAAATGGATGTATTTGGGAGCAGTTTGAGGTTTATAGGCACCTCAAATCTCTTGGTTTCATTATTGGAAATCATAAAGTTCCTTGGTCTGTGAAGGGTGTTAGGAATGGAAATGACATTTCTTCTCGAAGTTCTATATTTGAGAACAAAGGAGCGATAAATTTTGAATCAGAAGATGAGAGGTCGATCTCTGAGCTATTAGATTCCACTCAACTCAATGAAGTGACACCCATTTTTGATGTTTTTCTTCCACATAGCAAGTTTAGAAAATCTTCTCCTGGTGACACAAATTTTATGGTCTGCTCGACTAGGTAAATTCTTTTGCCAAAAATCATTGTAACTTCCTTCAGATTCTGTCCTATTGGTTATTTGTTTTGCATGTTGTCTTTGTTATTTTTGTATAAACTTCTTGGAAGGCATAGCTAAAAATGACTTGCACCTGATTTTCCTTTTTTAAGAACTATTGTCCTCTGATTTGACTTTTTAATGAAATTTCTTTTCAAAATAATATTATTAATAATAATAATAGTAATAATAATAATAATTTGCAAAAGGTGTTTTTTAGTGAGGCATGTAGGAATAGGATGTAAGCAGATTTGTTGTGAAATTGTATACACAATAGATTAACTTCATTTTCAGCCTTCTGAGTATTTCGCATCATTTTTTAGGAAAATAGTAGGTTGTATTCTTGAAATAACACCAAAATGAGGTCTATAATAGGCTTCAACTTGTGTCGTTTCTTTCTTCTGCAAAATTTTTATAGATTATTCAACTTATTTGTATGATCCTTTAATTACTGTGATATTATTTTTTTTATAGGGGATACCCACCTCCAAGAAAAATATTGAAGTTATTGATAGAACATCGAGAGGCAATCGAATGAAATATTGTCATGTTGAACATGGACGTGTTTGTTTCTTCTCACTTGATAAGGTGGAGTTGCCCGTCTTACCGTGA

mRNA sequence

ATGACGACATTTACTGAGCAAAACATGAGAGATGAAGAAGAATGTCTTTGTTCATTTTTAAATTTTTACATGCATGAAGTTGCAATTCAGAAGCATGCTTCGACTACTCGATGGAATGATCAGATGGGAATGGCAGAAGTTGTAGAGAACAAGGCGACCTTTGAGCGACGGCGGCATTGTACGTTGTGGCAAGATTTATTATTTCTTATTGAAGTTGGGGCCTTGCATCTTCTTGATTATGATAATTTAAATCTTTCTTTGAAAGACGTATACAAGAAGGTAGCTGAAGGAAAAAATGGATGTATTTGGGAGCAGTTTGAGGTTTATAGGCACCTCAAATCTCTTGGTTTCATTATTGGAAATCATAAAGTTCCTTGGTCTGTGAAGGGTGTTAGGAATGGAAATGACATTTCTTCTCGAAGTTCTATATTTGAGAACAAAGGAGCGATAAATTTTGAATCAGAAGATGAGAGGTCGATCTCTGAGCTATTAGATTCCACTCAACTCAATGAAGTGACACCCATTTTTGATGTTTTTCTTCCACATAGCAAGTTTAGAAAATCTTCTCCTGGTGACACAAATTTTATGGGGATACCCACCTCCAAGAAAAATATTGAAGTTATTGATAGAACATCGAGAGGCAATCGAATGAAATATTGTCATGTTGAACATGGACGTGTTTGTTTCTTCTCACTTGATAAGGTGGAGTTGCCCGTCTTACCGTGA

Coding sequence (CDS)

ATGACGACATTTACTGAGCAAAACATGAGAGATGAAGAAGAATGTCTTTGTTCATTTTTAAATTTTTACATGCATGAAGTTGCAATTCAGAAGCATGCTTCGACTACTCGATGGAATGATCAGATGGGAATGGCAGAAGTTGTAGAGAACAAGGCGACCTTTGAGCGACGGCGGCATTGTACGTTGTGGCAAGATTTATTATTTCTTATTGAAGTTGGGGCCTTGCATCTTCTTGATTATGATAATTTAAATCTTTCTTTGAAAGACGTATACAAGAAGGTAGCTGAAGGAAAAAATGGATGTATTTGGGAGCAGTTTGAGGTTTATAGGCACCTCAAATCTCTTGGTTTCATTATTGGAAATCATAAAGTTCCTTGGTCTGTGAAGGGTGTTAGGAATGGAAATGACATTTCTTCTCGAAGTTCTATATTTGAGAACAAAGGAGCGATAAATTTTGAATCAGAAGATGAGAGGTCGATCTCTGAGCTATTAGATTCCACTCAACTCAATGAAGTGACACCCATTTTTGATGTTTTTCTTCCACATAGCAAGTTTAGAAAATCTTCTCCTGGTGACACAAATTTTATGGGGATACCCACCTCCAAGAAAAATATTGAAGTTATTGATAGAACATCGAGAGGCAATCGAATGAAATATTGTCATGTTGAACATGGACGTGTTTGTTTCTTCTCACTTGATAAGGTGGAGTTGCCCGTCTTACCGTGA

Protein sequence

MTTFTEQNMRDEEECLCSFLNFYMHEVAIQKHASTTRWNDQMGMAEVVENKATFERRRHCTLWQDLLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNGCIWEQFEVYRHLKSLGFIIGNHKVPWSVKGVRNGNDISSRSSIFENKGAINFESEDERSISELLDSTQLNEVTPIFDVFLPHSKFRKSSPGDTNFMGIPTSKKNIEVIDRTSRGNRMKYCHVEHGRVCFFSLDKVELPVLP
Homology
[Close]
BLAST of Tan0012421 vs. NCBI nr
Match: XP_022156818.1 (uncharacterized protein LOC111023660 [Momordica charantia])

HSP 1 Score: 339.0 bits (868), Expect = 3.4e-89
Identity = 171/249 (68.67%), Postives = 203/249 (81.53%), Query Frame = 0 

Query: 6   EQNMRDEEECLCSFLNFYMHEVAIQKHASTTRWNDQMGMAEVVENKATFER--------R 65
           EQ++ DEEECLC+  N  M ++  +KHAST RWNDQMGMAEV+EN+ +           +
Sbjct: 21  EQDVEDEEECLCASGN--MRKLQFRKHASTARWNDQMGMAEVLENRGSLWTTTGIVRCGK 80

Query: 66  RHCTLWQDLLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNGCIWEQFEVYRHLKSLGF 125
            +C+ +++ LFL+EVGALHLLD+DN +LSLKDVYKKVAEGKNGC+WEQFEVYRHLKSLGF
Sbjct: 81  IYCS-FEETLFLMEVGALHLLDHDNSSLSLKDVYKKVAEGKNGCLWEQFEVYRHLKSLGF 140

Query: 126 IIGNHKVPWSVKGVRNGNDISSRSSIFENKGAINFESEDERSISELLDSTQLNEVTPIFD 185
           I+G HKVPWSVKGVRNG+DIS +SSI EN+GA + ES+DERSISELL S QL++V PIFD
Sbjct: 141 IVGKHKVPWSVKGVRNGSDISPQSSI-ENEGAWDLESKDERSISELLSSIQLDQVMPIFD 200

Query: 186 VFLPHSKFRKSSPGDTNFM-----GIPTSKKNIEVIDRTSRGNRMKYCHVEHGRVCFFSL 242
           VFLPHSKFRKSSPGD NFM     G P  KK+IE ++RTSRG  +KYCHVEHGRVCFFS 
Sbjct: 201 VFLPHSKFRKSSPGDPNFMVCLTRGYPPPKKDIEFLERTSRGIHIKYCHVEHGRVCFFSF 260

[Close]
BLAST of Tan0012421 vs. NCBI nr
Match: XP_023538818.1 (tRNA-splicing endonuclease subunit Sen54-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 337.8 bits (865), Expect = 7.7e-89
Identity = 172/249 (69.08%), Postives = 201/249 (80.72%), Query Frame = 0 

Query: 6   EQNMRDEEECLCSFLNFYMHEVAIQKHASTTRWNDQMGMAEVVENKATFER--------R 65
           EQ++++EEECLCS  N  M ++  +KHAST RWND+MGMAEV+ENK +           +
Sbjct: 21  EQDIKEEEECLCSSGN--MRKLQFRKHASTARWNDEMGMAEVLENKGSLWTTSGIVRCGK 80

Query: 66  RHCTLWQDLLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNGCIWEQFEVYRHLKSLGF 125
            +C++ ++ LFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ CIWEQFEVYRHLKSLG+
Sbjct: 81  IYCSI-EETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSECIWEQFEVYRHLKSLGY 140

Query: 126 IIGNHKVPWSVKGVRNGNDISSRSSIFENKGAINFESEDERSISELLDSTQLNEVTPIFD 185
           I+G HKVPWSVKG RNG DISSRSSI ENKG+ +F SEDE+SI EL+D+ QLNEVTPIFD
Sbjct: 141 IVGKHKVPWSVKGARNGGDISSRSSI-ENKGSTDFGSEDEKSICELIDAIQLNEVTPIFD 200

Query: 186 VFLPHSKFRKSSPGDTNFM-----GIPTSKKNIEVIDRTSRGNRMKYCHVEHGRVCFFSL 242
           V+LPHSKFRKSSPGD NFM     G P  K +IEVI+R S G  MKYCHVEHGRVCFFS 
Sbjct: 201 VYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGIPMKYCHVEHGRVCFFSF 260

[Close]
BLAST of Tan0012421 vs. NCBI nr
Match: KAG7028441.1 (tRNA-splicing endonuclease subunit Sen54 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 336.3 bits (861), Expect = 2.2e-88
Identity = 171/249 (68.67%), Postives = 201/249 (80.72%), Query Frame = 0 

Query: 6   EQNMRDEEECLCSFLNFYMHEVAIQKHASTTRWNDQMGMAEVVENKATFER--------R 65
           EQ++++EEECLCS  N  M ++  +KHAST RWND+MGMAEV+ENK +           +
Sbjct: 21  EQDIKEEEECLCSSGN--MRKLQFRKHASTARWNDEMGMAEVLENKGSLWTTSGIVRCGK 80

Query: 66  RHCTLWQDLLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNGCIWEQFEVYRHLKSLGF 125
            +C++ ++ LFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ CIWEQFEVYRHLKSLG+
Sbjct: 81  IYCSI-EETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSECIWEQFEVYRHLKSLGY 140

Query: 126 IIGNHKVPWSVKGVRNGNDISSRSSIFENKGAINFESEDERSISELLDSTQLNEVTPIFD 185
           I+G HKVPWSVKG RNG DISS+SSI ENKG+ +F SEDE+SI EL+D+ QLNEVTPIFD
Sbjct: 141 IVGKHKVPWSVKGARNGGDISSQSSI-ENKGSTDFGSEDEKSICELIDAIQLNEVTPIFD 200

Query: 186 VFLPHSKFRKSSPGDTNFM-----GIPTSKKNIEVIDRTSRGNRMKYCHVEHGRVCFFSL 242
           V+LPHSKFRKSSPGD NFM     G P  K +IEVI+R S G  MKYCHVEHGRVCFFS 
Sbjct: 201 VYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGIPMKYCHVEHGRVCFFSF 260

[Close]
BLAST of Tan0012421 vs. NCBI nr
Match: KAG6596967.1 (Chromatin assembly factor 1 subunit FAS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 336.3 bits (861), Expect = 2.2e-88
Identity = 171/249 (68.67%), Postives = 201/249 (80.72%), Query Frame = 0 

Query: 6   EQNMRDEEECLCSFLNFYMHEVAIQKHASTTRWNDQMGMAEVVENKATFER--------R 65
           EQ++++EEECLCS  N  M ++  +KHAST RWND+MGMAEV+ENK +           +
Sbjct: 292 EQDIKEEEECLCSSGN--MRKLQFRKHASTARWNDEMGMAEVLENKGSLWTTSGIVRCGK 351

Query: 66  RHCTLWQDLLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNGCIWEQFEVYRHLKSLGF 125
            +C++ ++ LFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ CIWEQFEVYRHLKSLG+
Sbjct: 352 IYCSI-EETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSECIWEQFEVYRHLKSLGY 411

Query: 126 IIGNHKVPWSVKGVRNGNDISSRSSIFENKGAINFESEDERSISELLDSTQLNEVTPIFD 185
           I+G HKVPWSVKG RNG DISS+SSI ENKG+ +F SEDE+SI EL+D+ QLNEVTPIFD
Sbjct: 412 IVGKHKVPWSVKGARNGGDISSQSSI-ENKGSTDFGSEDEKSICELIDAIQLNEVTPIFD 471

Query: 186 VFLPHSKFRKSSPGDTNFM-----GIPTSKKNIEVIDRTSRGNRMKYCHVEHGRVCFFSL 242
           V+LPHSKFRKSSPGD NFM     G P  K +IEVI+R S G  MKYCHVEHGRVCFFS 
Sbjct: 472 VYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGIPMKYCHVEHGRVCFFSF 531

[Close]
BLAST of Tan0012421 vs. NCBI nr
Match: XP_022940787.1 (tRNA-splicing endonuclease subunit Sen54 [Cucurbita moschata])

HSP 1 Score: 335.1 bits (858), Expect = 5.0e-88
Identity = 170/249 (68.27%), Postives = 201/249 (80.72%), Query Frame = 0 

Query: 6   EQNMRDEEECLCSFLNFYMHEVAIQKHASTTRWNDQMGMAEVVENKATFER--------R 65
           EQ++++EEECLCS  N  M ++  +KHAST RWND+MGMAEV+ENK +           +
Sbjct: 21  EQDIKEEEECLCSSGN--MRKLQFRKHASTARWNDEMGMAEVLENKGSLWTTSGIVRCGK 80

Query: 66  RHCTLWQDLLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNGCIWEQFEVYRHLKSLGF 125
            +C++ ++ LFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ CIWEQFEVYRHLKSLG+
Sbjct: 81  IYCSI-EETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSECIWEQFEVYRHLKSLGY 140

Query: 126 IIGNHKVPWSVKGVRNGNDISSRSSIFENKGAINFESEDERSISELLDSTQLNEVTPIFD 185
           I+G HKVPWSVKG +NG DISS+SSI ENKG+ +F SEDE+SI EL+D+ QLNEVTPIFD
Sbjct: 141 IVGKHKVPWSVKGAKNGGDISSQSSI-ENKGSTDFGSEDEKSICELIDAIQLNEVTPIFD 200

Query: 186 VFLPHSKFRKSSPGDTNFM-----GIPTSKKNIEVIDRTSRGNRMKYCHVEHGRVCFFSL 242
           V+LPHSKFRKSSPGD NFM     G P  K +IEVI+R S G  MKYCHVEHGRVCFFS 
Sbjct: 201 VYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGIPMKYCHVEHGRVCFFSF 260

[Close]
BLAST of Tan0012421 vs. ExPASy TrEMBL
Match: A0A6J1DRN3 (uncharacterized protein LOC111023660 OS=Momordica charantia OX=3673 GN=LOC111023660 PE=3 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 1.7e-89
Identity = 171/249 (68.67%), Postives = 203/249 (81.53%), Query Frame = 0 

Query: 6   EQNMRDEEECLCSFLNFYMHEVAIQKHASTTRWNDQMGMAEVVENKATFER--------R 65
           EQ++ DEEECLC+  N  M ++  +KHAST RWNDQMGMAEV+EN+ +           +
Sbjct: 21  EQDVEDEEECLCASGN--MRKLQFRKHASTARWNDQMGMAEVLENRGSLWTTTGIVRCGK 80

Query: 66  RHCTLWQDLLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNGCIWEQFEVYRHLKSLGF 125
            +C+ +++ LFL+EVGALHLLD+DN +LSLKDVYKKVAEGKNGC+WEQFEVYRHLKSLGF
Sbjct: 81  IYCS-FEETLFLMEVGALHLLDHDNSSLSLKDVYKKVAEGKNGCLWEQFEVYRHLKSLGF 140

Query: 126 IIGNHKVPWSVKGVRNGNDISSRSSIFENKGAINFESEDERSISELLDSTQLNEVTPIFD 185
           I+G HKVPWSVKGVRNG+DIS +SSI EN+GA + ES+DERSISELL S QL++V PIFD
Sbjct: 141 IVGKHKVPWSVKGVRNGSDISPQSSI-ENEGAWDLESKDERSISELLSSIQLDQVMPIFD 200

Query: 186 VFLPHSKFRKSSPGDTNFM-----GIPTSKKNIEVIDRTSRGNRMKYCHVEHGRVCFFSL 242
           VFLPHSKFRKSSPGD NFM     G P  KK+IE ++RTSRG  +KYCHVEHGRVCFFS 
Sbjct: 201 VFLPHSKFRKSSPGDPNFMVCLTRGYPPPKKDIEFLERTSRGIHIKYCHVEHGRVCFFSF 260

[Close]
BLAST of Tan0012421 vs. ExPASy TrEMBL
Match: A0A6J1FKK4 (tRNA-splicing endonuclease subunit Sen54 OS=Cucurbita moschata OX=3662 GN=LOC111446255 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 2.4e-88
Identity = 170/249 (68.27%), Postives = 201/249 (80.72%), Query Frame = 0 

Query: 6   EQNMRDEEECLCSFLNFYMHEVAIQKHASTTRWNDQMGMAEVVENKATFER--------R 65
           EQ++++EEECLCS  N  M ++  +KHAST RWND+MGMAEV+ENK +           +
Sbjct: 21  EQDIKEEEECLCSSGN--MRKLQFRKHASTARWNDEMGMAEVLENKGSLWTTSGIVRCGK 80

Query: 66  RHCTLWQDLLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNGCIWEQFEVYRHLKSLGF 125
            +C++ ++ LFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ CIWEQFEVYRHLKSLG+
Sbjct: 81  IYCSI-EETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSECIWEQFEVYRHLKSLGY 140

Query: 126 IIGNHKVPWSVKGVRNGNDISSRSSIFENKGAINFESEDERSISELLDSTQLNEVTPIFD 185
           I+G HKVPWSVKG +NG DISS+SSI ENKG+ +F SEDE+SI EL+D+ QLNEVTPIFD
Sbjct: 141 IVGKHKVPWSVKGAKNGGDISSQSSI-ENKGSTDFGSEDEKSICELIDAIQLNEVTPIFD 200

Query: 186 VFLPHSKFRKSSPGDTNFM-----GIPTSKKNIEVIDRTSRGNRMKYCHVEHGRVCFFSL 242
           V+LPHSKFRKSSPGD NFM     G P  K +IEVI+R S G  MKYCHVEHGRVCFFS 
Sbjct: 201 VYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGIPMKYCHVEHGRVCFFSF 260

[Close]
BLAST of Tan0012421 vs. ExPASy TrEMBL
Match: A0A6J1I8D9 (tRNA-splicing endonuclease subunit Sen54 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111471897 PE=3 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.6e-87
Identity = 170/249 (68.27%), Postives = 201/249 (80.72%), Query Frame = 0 

Query: 6   EQNMRDEEECLCSFLNFYMHEVAIQKHASTTRWNDQMGMAEVVENKATFER--------R 65
           EQ++++EEECL S  N  M ++  +KHAST RWND+MGMAEV+ENK +           +
Sbjct: 21  EQDIKEEEECLRSSGN--MRKLQFRKHASTARWNDEMGMAEVLENKGSLWTTSGIVRCGK 80

Query: 66  RHCTLWQDLLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNGCIWEQFEVYRHLKSLGF 125
            +C++ ++ LFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ CIWEQFEVYRHLKSLG+
Sbjct: 81  IYCSI-EETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSECIWEQFEVYRHLKSLGY 140

Query: 126 IIGNHKVPWSVKGVRNGNDISSRSSIFENKGAINFESEDERSISELLDSTQLNEVTPIFD 185
           I+G HKVPWSVKG RNG DISSRSSI ENKG+ +FESEDE+SI ELL++ QLNE+TPIFD
Sbjct: 141 IVGKHKVPWSVKGARNGGDISSRSSI-ENKGSTDFESEDEKSICELLNAVQLNELTPIFD 200

Query: 186 VFLPHSKFRKSSPGDTNFM-----GIPTSKKNIEVIDRTSRGNRMKYCHVEHGRVCFFSL 242
           V+LPHSKFRKSSPGD NFM     G P  K +IEVI+R S G  MKYCHVEHGRVCFFS 
Sbjct: 201 VYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGIPMKYCHVEHGRVCFFSF 260

[Close]
BLAST of Tan0012421 vs. ExPASy TrEMBL
Match: A0A6J1ICR8 (tRNA-splicing endonuclease subunit Sen54 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111471897 PE=3 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.6e-87
Identity = 170/249 (68.27%), Postives = 201/249 (80.72%), Query Frame = 0 

Query: 6   EQNMRDEEECLCSFLNFYMHEVAIQKHASTTRWNDQMGMAEVVENKATFER--------R 65
           EQ++++EEECL S  N  M ++  +KHAST RWND+MGMAEV+ENK +           +
Sbjct: 25  EQDIKEEEECLRSSGN--MRKLQFRKHASTARWNDEMGMAEVLENKGSLWTTSGIVRCGK 84

Query: 66  RHCTLWQDLLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNGCIWEQFEVYRHLKSLGF 125
            +C++ ++ LFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ CIWEQFEVYRHLKSLG+
Sbjct: 85  IYCSI-EETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSECIWEQFEVYRHLKSLGY 144

Query: 126 IIGNHKVPWSVKGVRNGNDISSRSSIFENKGAINFESEDERSISELLDSTQLNEVTPIFD 185
           I+G HKVPWSVKG RNG DISSRSSI ENKG+ +FESEDE+SI ELL++ QLNE+TPIFD
Sbjct: 145 IVGKHKVPWSVKGARNGGDISSRSSI-ENKGSTDFESEDEKSICELLNAVQLNELTPIFD 204

Query: 186 VFLPHSKFRKSSPGDTNFM-----GIPTSKKNIEVIDRTSRGNRMKYCHVEHGRVCFFSL 242
           V+LPHSKFRKSSPGD NFM     G P  K +IEVI+R S G  MKYCHVEHGRVCFFS 
Sbjct: 205 VYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGIPMKYCHVEHGRVCFFSF 264

[Close]
BLAST of Tan0012421 vs. ExPASy TrEMBL
Match: A0A6J1I794 (tRNA-splicing endonuclease subunit Sen54 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471897 PE=3 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.6e-87
Identity = 170/249 (68.27%), Postives = 201/249 (80.72%), Query Frame = 0 

Query: 6   EQNMRDEEECLCSFLNFYMHEVAIQKHASTTRWNDQMGMAEVVENKATFER--------R 65
           EQ++++EEECL S  N  M ++  +KHAST RWND+MGMAEV+ENK +           +
Sbjct: 35  EQDIKEEEECLRSSGN--MRKLQFRKHASTARWNDEMGMAEVLENKGSLWTTSGIVRCGK 94

Query: 66  RHCTLWQDLLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNGCIWEQFEVYRHLKSLGF 125
            +C++ ++ LFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ CIWEQFEVYRHLKSLG+
Sbjct: 95  IYCSI-EETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSECIWEQFEVYRHLKSLGY 154

Query: 126 IIGNHKVPWSVKGVRNGNDISSRSSIFENKGAINFESEDERSISELLDSTQLNEVTPIFD 185
           I+G HKVPWSVKG RNG DISSRSSI ENKG+ +FESEDE+SI ELL++ QLNE+TPIFD
Sbjct: 155 IVGKHKVPWSVKGARNGGDISSRSSI-ENKGSTDFESEDEKSICELLNAVQLNELTPIFD 214

Query: 186 VFLPHSKFRKSSPGDTNFM-----GIPTSKKNIEVIDRTSRGNRMKYCHVEHGRVCFFSL 242
           V+LPHSKFRKSSPGD NFM     G P  K +IEVI+R S G  MKYCHVEHGRVCFFS 
Sbjct: 215 VYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGIPMKYCHVEHGRVCFFSF 274

[Close]
BLAST of Tan0012421 vs. TAIR 10
Match: AT3G57360.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G02370.2); Has 122 Blast hits to 122 proteins in 54 species: Archae - 0; Bacteria - 0; Metazoa - 74; Fungi - 4; Plants - 41; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 144.8 bits (364), Expect = 8.9e-35
Identity = 79/223 (35.43%), Postives = 130/223 (58.30%), Query Frame = 0 

Query: 33  ASTTRWNDQMGMAEVVENKATF--------ERRRHCTLWQDLLFLIEVGALHLL-DYDNL 92
           +S  RW  ++GMAEV   +             + +C + ++ L+L E+G L +L + D++
Sbjct: 41  SSKARWITELGMAEVEVKRGKLWTTTGIIRSGKTYCFI-EEALYLSEIGELQILGNEDDI 100

Query: 93  NLSLKDVYKKVAEGKNGCIWEQFEVYRHLKSLGFIIGNHKVPWSVKGVRNGNDISSRSSI 152
            + LKD+Y+K+AE K+GC WE +EVYR+LK LG+I+G H V W++K     N        
Sbjct: 101 VIPLKDLYEKIAEEKSGCSWENYEVYRYLKGLGYILGRHGVSWTLKDAARPNG------- 160

Query: 153 FENKGAINFE-SEDERSISELLDSTQLNEVTPIFDVFLPHSKFRKSSPGDTNFMGI---- 212
            E + A   E   D  ++++LL   Q+ +   +FDV+LP+S+F+KSSPG+ +F+      
Sbjct: 161 -EEESACAGECPADNDTVTKLLGDMQICDAKAVFDVYLPNSRFKKSSPGEPSFVACFSGD 220

Query: 213 -PTSKKNIEVIDRTSRGNRMKYCHVEHGRVCFFSLDKVELPVL 241
            P SK++I+V+ +      + +CH+  GR  FFS   ++LPVL
Sbjct: 221 SPPSKEDIKVLQKRIAA-PLTFCHIAQGRASFFSFSSIDLPVL 253

[Close]
BLAST of Tan0012421 vs. TAIR 10
Match: AT3G02370.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G57360.1). )

HSP 1 Score: 119.8 bits (299), Expect = 3.1e-27
Identity = 64/149 (42.95%), Postives = 98/149 (65.77%), Query Frame = 0 

Query: 64  QDLLFLIEVGALHLL-DYDNLNLSLKDVYKKVAEGKNGCIWEQFEVYRHLKSLGFIIGNH 123
           ++ L+L E+G L LL D D++ +SLKD+Y ++AEGK GC WE +EVYR+LK LG+I+G H
Sbjct: 28  EEALYLSEIGELQLLGDEDDVVISLKDLYGEIAEGKYGCCWENYEVYRYLKGLGYILGRH 87

Query: 124 KVPWSVKGVRNGNDISSRSSIFENKGAINFESEDERSISELLDSTQLNEVTPIFDVFLPH 183
            VPW+ K   N    ++ S   E+  A  F  +D  S+++LL    + +  P+FDV+LP+
Sbjct: 88  GVPWTTKYAVN----TTPSDEDESLCAAEF-FQDRDSVTKLLSDMHICDARPVFDVYLPN 147

Query: 184 SKFRKSSPGDTNFM-----GIPTSKKNIE 207
           S+F+KSSPG+ +F+       P SK+ I+
Sbjct: 148 SQFKKSSPGEPSFVTCFSGDSPPSKEEIK 171

[Close]
BLAST of Tan0012421 vs. TAIR 10
Match: AT3G02370.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G57360.1); Has 95 Blast hits to 95 proteins in 40 species: Archae - 0; Bacteria - 0; Metazoa - 53; Fungi - 2; Plants - 39; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 119.4 bits (298), Expect = 4.0e-27
Identity = 60/134 (44.78%), Postives = 92/134 (68.66%), Query Frame = 0 

Query: 64  QDLLFLIEVGALHLL-DYDNLNLSLKDVYKKVAEGKNGCIWEQFEVYRHLKSLGFIIGNH 123
           ++ L+L E+G L LL D D++ +SLKD+Y ++AEGK GC WE +EVYR+LK LG+I+G H
Sbjct: 28  EEALYLSEIGELQLLGDEDDVVISLKDLYGEIAEGKYGCCWENYEVYRYLKGLGYILGRH 87

Query: 124 KVPWSVKGVRNGNDISSRSSIFENKGAINFESEDERSISELLDSTQLNEVTPIFDVFLPH 183
            VPW+ K   N    ++ S   E+  A  F  +D  S+++LL    + +  P+FDV+LP+
Sbjct: 88  GVPWTTKYAVN----TTPSDEDESLCAAEF-FQDRDSVTKLLSDMHICDARPVFDVYLPN 147

Query: 184 SKFRKSSPGDTNFM 197
           S+F+KSSPG+ +F+
Sbjct: 148 SQFKKSSPGEPSFV 156

The following BLAST results are available for this feature:
BLAST of Tan0012421 vs.
Match NameE-valueIdentityDescription
BLAST of Tan0012421 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022156818.13.4e-8968.67uncharacterized protein LOC111023660 [Momordica charantia][more]
XP_023538818.17.7e-8969.08tRNA-splicing endonuclease subunit Sen54-like [Cucurbita pepo subsp. pepo][more]
KAG7028441.12.2e-8868.67tRNA-splicing endonuclease subunit Sen54 [Cucurbita argyrosperma subsp. argyrosp... [more]
KAG6596967.12.2e-8868.67Chromatin assembly factor 1 subunit FAS1, partial [Cucurbita argyrosperma subsp.... [more]
XP_022940787.15.0e-8868.27tRNA-splicing endonuclease subunit Sen54 [Cucurbita moschata][more]
BLAST of Tan0012421 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DRN31.7e-8968.67uncharacterized protein LOC111023660 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1FKK42.4e-8868.27tRNA-splicing endonuclease subunit Sen54 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1I8D91.6e-8768.27tRNA-splicing endonuclease subunit Sen54 isoform X3 OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1ICR81.6e-8768.27tRNA-splicing endonuclease subunit Sen54 isoform X2 OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1I7941.6e-8768.27tRNA-splicing endonuclease subunit Sen54 isoform X1 OS=Cucurbita maxima OX=3661 ... [more]
BLAST of Tan0012421 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G57360.18.9e-3535.43unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G02370.23.1e-2742.95unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT3G02370.14.0e-2744.78unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024337tRNA-splicing endonuclease, subunit Sen54PANTHERPTHR21027TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54coord: 24..241

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0012421.1Tan0012421.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0000379 tRNA-type intron splice site recognition and cleavage
cellular_component GO:0000214 tRNA-intron endonuclease complex
molecular_function GO:0004519 endonuclease activity