Tan0012345 (gene) Snake gourd v1

Overview


Name	Tan0012345
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Asparagine--tRNA ligase
Location	LG06: 12403546 .. 12408293 (-)
RNA-Seq Expression	Tan0012345
Synteny	Tan0012345

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCGAAAACCAAAACACACAAAAACTGAAATGAACAACTCTCCAAGAAACTCCCAATCTCCAACTCAAACGATTCCAATTCAAACTCTGTAAATCTCTCTCAGTTCATCCCTCAAACACACACCGCAAATCATTTCTCTGAAACAATCCCTTTCCTTCCATTAACACCCTCTCTCGATTTCCCCCTCTTCTTCCCCAAGAAAAGCATCTCAATCTTCTTCTATATCTCTCCTTCCATGTCTCATCTCCCTCACCCATCAATTCCCCTTTCCAATTCCCCATTTCTCTCCTCTTTCTTCCTCCAATGGCCGCCCAACACCCCCCACTCTTCACCCCCCTCACCTTCTCCAAGTATTCGAAGCGGGTTCTTCTCAAAACTGTGCTCGACAGCACCGACGGGGGTCTCGGGTTTGTGGATCACAAATTGGTGGTTGGAGGCTGGGTGAAGTCCTCCAAAGAGGTCGTCAGAGAGGCTCCGTCGCCGGCTGATGCCACTGTCAGGGCGGCCGGTGAGGCCCTGCCGCCCAGGGCTAAGGATGTGAGCTGCATTGAGCTTATACAGTCGCGGATTCCTATCGTTAGGTCTTTGCTTAAGATGCTGTATGGAAATAATTTACACGGCGGTGAGAAATTGGAGACGCCTGTTCAGAAGCCGCCGCTTCCTTCCACTGCTTTTCTGGTTGTTAATGACGGCTCGTGTGTTGCAAGCCTTCAGGTACCCTCTAATTTTATGCACGCCAACTGTTTGTAAGTTTGTTACTTATGGTATAGGATTTGGTTATCTTAAAGTTTGTATCCAATTTTACTCTTTATGTCTTTTTATTCTTTATCTTAAAGTTTCATATTCTTTTTATGCCTAACAAGTGTTTGATTTTTGTCTTACTGAGTGTTTGATATACGTCGGACATGGATATGTTACCCAAACTAAATGATAATAAAATCTATATTTTACCCTTTATATTGCTTGTATGCATACATCTGCAATGGAGATTTAAAGAATTTACTGTGTTGGTGTGCATAGGGATGCATTTTTATGTTATCCCCCAACCCACTAGAAAAGAGAGAAGAAAATGAGGAAATAAAGAACTTTTAACAAAATTTAAAGTTGATTCAGAGTTAAGGAAGGGTCTAATATTTTTCATGAGCACTTGTTAGAGGATAATTGGTTGCTTAAGCTGTGGATATGCTTGGCTTTTTGCATTCACATAATGACAAGAAACTTGCAGGTTGTTATTGAGTCCTCGTTACATCATCCTGGCCAGCTGATACCAACGGGAACTTGCATCTTGGTGGAAGGCATATTGAAGCGGGCACCGGTACCTGGGAAACATGTTGTTCGGCTTGAAGTGGAGAAAATCCTTCATGTAGGGAAGGTTGAACAGGAAAAGTATCCATTATCCCAAAAGAAGTTGCCAATGGATTTTTTGAGGAAATTTTCTTACTTTCGTCCACGAACAACTACGGTAATATGCACAAGCTTCAACTATACATTGCATTTATTTCCTATTTTTGTGGTTCTGTTTGCATGCCTATTATTAAAGCGCCTATTAGTTTTAAGATTGTGCTTAAGCAATAGCAGCATGTGAATTAGAATAAAGGTTTCCATTCATATGTTATATCCGGTGATTCATAAAGTAGAGACTTGAGATCAGGAAAATGGAGATTTATACAGTTCAAATTGTGTATATAGTTCGATGAGATCATGGCTTAAAGTGAAGTAAAAACTTTCACTTCTTTGTAGATCTCTTTGGTAGGATTGCTTGCATTGACCTATATAGCACCTGTGCGAAGTGCTCTGGTGCTAAAGCTTGAGAAGAAAGTTTTGCATATTTAATCGTTGAAACCAGTGAAGTATTGGACCTTGATCTTCATTGATATTCTTTACTTTATTCAGGTAGCATCTGTTATGAGAATCCGTAATGCCTTGACTTTTGCAACTCACACGTTCTTCCAAAACCATGGCTTTCTTTATGTGCAATTACCCACAATCACAACCACTGACAGCGAGGGATTCAGTGAAAAGTTCCACGTTACAACAGTTGCCGGTAAATCAGCAGGTAAGAAAGTAGAACCAACAGAAGATAAAGAAACTGATGGTGTGAGTCTTGAAACTGTAAAGGCTGCTGCAATAGAGAAAAGCAATCTCGTCAAAGAACTCGAGAGAAGTGAAAGCAACAGGGAAGCCTTAGTTGCTGCAGTTCAGGATTTAAAGAAAACAAATGAACTTGCATTACAATTAGAAGCCAGAGGAAAATTAAAATCCAAATCGAAGAAAGATAAAGTTCACTTTTCTGAAAATTTCTTCCCACAAGAAACTTATCTTACGGTTTCTGGACAACTCCATTTGGAGACCTATGCGTGTGCCCTTGGCAATGTTTACTCATGTGGACCAAGGTTTCGAGCAGATAAAATTGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAGGTTGAAATTGCTTTTGCTGAACTTGAGGTATGAATTTATGATCTTTTACTTATTCCTTTAGATTGTGCTTAGTATTTTACATCTAAGGCTCCGTTTGATAACAATTTTGTTTTTGGTTTTTAGTTTTTGAAATTTAAACCTAAAAATACTACTTTTACCAATGAGTTTATATGTATTCTTATCTACTTTGTATCTATGTTTTCAAAAACTAAGCCAACTTTTGAAAACAAAAAAAGTAGCTTTCAAAAACTTGAATTTGTTTTTAGAATTTGGCTAAGAGTTCAAATGCTTACTTAAGGGAGATGATCTCCATTGTAGAGAAATTGGGTGAAAATAAGCTTAACTTTTAAAAACTAAAAACGAAAAACAAAATGGTTATCAAACGGGGCCTAAATTATCTGTTTAAGATTCCAGGTTGATTTAGCCATTAAAACCTTTATTTTCTGTGATCAGTAGTTGTAATATCATTGCTGATTGCACCATTGAAATGAAAATTTTGCATCCTTCTAACTTGCATTTTGTATAATTTGGGGAGTCCAGGATGTCATGAACTGTGCTGATGACCTTGTAAAGTTCCTCTCCAAATGGGTATTGGAGCACTGTCTGGATGATATGAAATTCGTGCAAAAACGAATCGACAAGACAAGCATTCATCGCCTTGAATCGTTTATCACAAGTTCATTTGAGAAGGTCTCTTACACTGCAGCCATAGAAATTCTGGATAAGGTGAAAATTCAGATTATCTAATCTTCTATCACGTGACTCAATTTTTCATTTCCACCACTGCCTATCTGAAGTTGAAAAAAAATATATCTTCACTGTCAGGCAACCGATCAAAAATCACAACACTTGAAAAAACTTAAATTTGGCGCTGCTTTAACTGCTGATCACCTCAGGTGTGTAAAATATGCTATTTCAGTCAATAAGCAAAATGAGTTTTTTTTTTTTTTCAATTAAAATTATTTCTTCTTTTTACATGAAACAGTTATCTAGCTGATGCCATCTATAAGAAGCCCGTGATAGTATTTAACTACCCAAAAGAAACCAAGCCATTTAATGTGCGTCTGAATGATGATGGGCAAACAGTAGCAGCATTTGATGTGGTTCTTCCAAAGGTTAGCACATGGGCATATATTGACTCTTGGGAGAATTTATTTTTGGATGGATTCTGTATTCAATGAAAATTTATTTCTTAAACTGGGCCGACTGTTTGTAGGGTGGAGTAGTAATTTCTGGCAGCCAAAAGGAGGAGCGTTTTGATGTATTGATCACAAGGTATGACATTTTAGTGGCTTGAATCCTATTTCGGTATATGGACTCTCTTGTTTATTTCATTTTGATCTTAAATATTCCATAATATAATTTTATTCACTGAACTTTACCTAAAAAAACCATTTTAGACTTTGTAATTATTTTTTCAATAAATTATTCAACAGAAACTTGGACATTGCCCACATTATTTTATTTATTTACTACGTCTACATAGACACATATTTATATTCACGTGTAGTAGGTTAGACGTCAAGGTTCCATTATGGATTTAGGTTTTTCTTTTTTAAGATAATTAATGAAACACTAACGGAAAGGGCTAAAGTAGTTTTTTATGCAAAATTTGGAGGAAAATACACAGAATATCTTAAAGTTGAGGGACCAAAAGCCAAATAAGTCTAATCATCATGAGTTGGTCTAGCGTCACTCGTGTCACTAGGGCATGTGAAACAAGTAAATGGACTTCGAAGAAATGGGTTCAAACTCCATGATAGCTACTTGAGATAAAATTTAACTAGGTTTTACTAGTTTCGTGATGGTTCTATGTTTTCATAACGCTCATGGATATAAAAACAAAACAAGATAAGCATGAAAGTTCCTCTTTTTAATATGGTTGTTGGCAGGATGAAGGAGATGGGTCTGCCAAGGGAGCAGTATGAGTGGTACTTGGAGATTCAAAGGCATGGAACAGTGAAACACTCTGGTTTTAGTTTGGCATTTGATCGTTTCGTTCTGTTTACTACTGGTCTCACTGATATTAAAGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGCCAGCAATTAAACCGAGTTCAAGGGCAAAATCGTCCAAAAATGTTTTTCGAAGCAGCTGGCAAGATCAATGGTGATGCTGAAATTGAACTGAAAATTGTGTTTGTTAGTGTAAAAGCCGAAGAAAAATGACTTGTGGTTGGGAGATTTGTGTTTGTTGCAAAGATTTGTTCTTGTAAATAAAAGAGAATTTGGAATCAAATGAGATATTTGTGGAGAAGCTCGGGTTCATATT

mRNA sequence

TCGAAAACCAAAACACACAAAAACTGAAATGAACAACTCTCCAAGAAACTCCCAATCTCCAACTCAAACGATTCCAATTCAAACTCTGTAAATCTCTCTCAGTTCATCCCTCAAACACACACCGCAAATCATTTCTCTGAAACAATCCCTTTCCTTCCATTAACACCCTCTCTCGATTTCCCCCTCTTCTTCCCCAAGAAAAGCATCTCAATCTTCTTCTATATCTCTCCTTCCATGTCTCATCTCCCTCACCCATCAATTCCCCTTTCCAATTCCCCATTTCTCTCCTCTTTCTTCCTCCAATGGCCGCCCAACACCCCCCACTCTTCACCCCCCTCACCTTCTCCAAGTATTCGAAGCGGGTTCTTCTCAAAACTGTGCTCGACAGCACCGACGGGGGTCTCGGGTTTGTGGATCACAAATTGGTGGTTGGAGGCTGGGTGAAGTCCTCCAAAGAGGTCGTCAGAGAGGCTCCGTCGCCGGCTGATGCCACTGTCAGGGCGGCCGGTGAGGCCCTGCCGCCCAGGGCTAAGGATGTGAGCTGCATTGAGCTTATACAGTCGCGGATTCCTATCGTTAGGTCTTTGCTTAAGATGCTGTATGGAAATAATTTACACGGCGGTGAGAAATTGGAGACGCCTGTTCAGAAGCCGCCGCTTCCTTCCACTGCTTTTCTGGTTGTTAATGACGGCTCGTGTGTTGCAAGCCTTCAGGTTGTTATTGAGTCCTCGTTACATCATCCTGGCCAGCTGATACCAACGGGAACTTGCATCTTGGTGGAAGGCATATTGAAGCGGGCACCGGTACCTGGGAAACATGTTGTTCGGCTTGAAGTGGAGAAAATCCTTCATGTAGGGAAGGTTGAACAGGAAAAGTATCCATTATCCCAAAAGAAGTTGCCAATGGATTTTTTGAGGAAATTTTCTTACTTTCGTCCACGAACAACTACGGTAGCATCTGTTATGAGAATCCGTAATGCCTTGACTTTTGCAACTCACACGTTCTTCCAAAACCATGGCTTTCTTTATGTGCAATTACCCACAATCACAACCACTGACAGCGAGGGATTCAGTGAAAAGTTCCACGTTACAACAGTTGCCGGTAAATCAGCAGGTAAGAAAGTAGAACCAACAGAAGATAAAGAAACTGATGGTGTGAGTCTTGAAACTGTAAAGGCTGCTGCAATAGAGAAAAGCAATCTCGTCAAAGAACTCGAGAGAAGTGAAAGCAACAGGGAAGCCTTAGTTGCTGCAGTTCAGGATTTAAAGAAAACAAATGAACTTGCATTACAATTAGAAGCCAGAGGAAAATTAAAATCCAAATCGAAGAAAGATAAAGTTCACTTTTCTGAAAATTTCTTCCCACAAGAAACTTATCTTACGGTTTCTGGACAACTCCATTTGGAGACCTATGCGTGTGCCCTTGGCAATGTTTACTCATGTGGACCAAGGTTTCGAGCAGATAAAATTGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAGGTTGAAATTGCTTTTGCTGAACTTGAGGATGTCATGAACTGTGCTGATGACCTTGTAAAGTTCCTCTCCAAATGGGTATTGGAGCACTGTCTGGATGATATGAAATTCGTGCAAAAACGAATCGACAAGACAAGCATTCATCGCCTTGAATCGTTTATCACAAGTTCATTTGAGAAGGTCTCTTACACTGCAGCCATAGAAATTCTGGATAAGGCAACCGATCAAAAATCACAACACTTGAAAAAACTTAAATTTGGCGCTGCTTTAACTGCTGATCACCTCAGTTATCTAGCTGATGCCATCTATAAGAAGCCCGTGATAGTATTTAACTACCCAAAAGAAACCAAGCCATTTAATGTGCGTCTGAATGATGATGGGCAAACAGTAGCAGCATTTGATGTGGTTCTTCCAAAGGGTGGAGTAGTAATTTCTGGCAGCCAAAAGGAGGAGCGTTTTGATGTATTGATCACAAGGATGAAGGAGATGGGTCTGCCAAGGGAGCAGTATGAGTGGTACTTGGAGATTCAAAGGCATGGAACAGTGAAACACTCTGGTTTTAGTTTGGCATTTGATCGTTTCGTTCTGTTTACTACTGGTCTCACTGATATTAAAGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGCCAGCAATTAAACCGAGTTCAAGGGCAAAATCGTCCAAAAATGTTTTTCGAAGCAGCTGGCAAGATCAATGGTGATGCTGAAATTGAACTGAAAATTGTGTTTGTTAGTGTAAAAGCCGAAGAAAAATGACTTGTGGTTGGGAGATTTGTGTTTGTTGCAAAGATTTGTTCTTGTAAATAAAAGAGAATTTGGAATCAAATGAGATATTTGTGGAGAAGCTCGGGTTCATATT

Coding sequence (CDS)

ATGGCCGCCCAACACCCCCCACTCTTCACCCCCCTCACCTTCTCCAAGTATTCGAAGCGGGTTCTTCTCAAAACTGTGCTCGACAGCACCGACGGGGGTCTCGGGTTTGTGGATCACAAATTGGTGGTTGGAGGCTGGGTGAAGTCCTCCAAAGAGGTCGTCAGAGAGGCTCCGTCGCCGGCTGATGCCACTGTCAGGGCGGCCGGTGAGGCCCTGCCGCCCAGGGCTAAGGATGTGAGCTGCATTGAGCTTATACAGTCGCGGATTCCTATCGTTAGGTCTTTGCTTAAGATGCTGTATGGAAATAATTTACACGGCGGTGAGAAATTGGAGACGCCTGTTCAGAAGCCGCCGCTTCCTTCCACTGCTTTTCTGGTTGTTAATGACGGCTCGTGTGTTGCAAGCCTTCAGGTTGTTATTGAGTCCTCGTTACATCATCCTGGCCAGCTGATACCAACGGGAACTTGCATCTTGGTGGAAGGCATATTGAAGCGGGCACCGGTACCTGGGAAACATGTTGTTCGGCTTGAAGTGGAGAAAATCCTTCATGTAGGGAAGGTTGAACAGGAAAAGTATCCATTATCCCAAAAGAAGTTGCCAATGGATTTTTTGAGGAAATTTTCTTACTTTCGTCCACGAACAACTACGGTAGCATCTGTTATGAGAATCCGTAATGCCTTGACTTTTGCAACTCACACGTTCTTCCAAAACCATGGCTTTCTTTATGTGCAATTACCCACAATCACAACCACTGACAGCGAGGGATTCAGTGAAAAGTTCCACGTTACAACAGTTGCCGGTAAATCAGCAGGTAAGAAAGTAGAACCAACAGAAGATAAAGAAACTGATGGTGTGAGTCTTGAAACTGTAAAGGCTGCTGCAATAGAGAAAAGCAATCTCGTCAAAGAACTCGAGAGAAGTGAAAGCAACAGGGAAGCCTTAGTTGCTGCAGTTCAGGATTTAAAGAAAACAAATGAACTTGCATTACAATTAGAAGCCAGAGGAAAATTAAAATCCAAATCGAAGAAAGATAAAGTTCACTTTTCTGAAAATTTCTTCCCACAAGAAACTTATCTTACGGTTTCTGGACAACTCCATTTGGAGACCTATGCGTGTGCCCTTGGCAATGTTTACTCATGTGGACCAAGGTTTCGAGCAGATAAAATTGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAGGTTGAAATTGCTTTTGCTGAACTTGAGGATGTCATGAACTGTGCTGATGACCTTGTAAAGTTCCTCTCCAAATGGGTATTGGAGCACTGTCTGGATGATATGAAATTCGTGCAAAAACGAATCGACAAGACAAGCATTCATCGCCTTGAATCGTTTATCACAAGTTCATTTGAGAAGGTCTCTTACACTGCAGCCATAGAAATTCTGGATAAGGCAACCGATCAAAAATCACAACACTTGAAAAAACTTAAATTTGGCGCTGCTTTAACTGCTGATCACCTCAGTTATCTAGCTGATGCCATCTATAAGAAGCCCGTGATAGTATTTAACTACCCAAAAGAAACCAAGCCATTTAATGTGCGTCTGAATGATGATGGGCAAACAGTAGCAGCATTTGATGTGGTTCTTCCAAAGGGTGGAGTAGTAATTTCTGGCAGCCAAAAGGAGGAGCGTTTTGATGTATTGATCACAAGGATGAAGGAGATGGGTCTGCCAAGGGAGCAGTATGAGTGGTACTTGGAGATTCAAAGGCATGGAACAGTGAAACACTCTGGTTTTAGTTTGGCATTTGATCGTTTCGTTCTGTTTACTACTGGTCTCACTGATATTAAAGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGCCAGCAATTAA

Protein sequence

MAAQHPPLFTPLTFSKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVREAPSPADATVRAAGEALPPRAKDVSCIELIQSRIPIVRSLLKMLYGNNLHGGEKLETPVQKPPLPSTAFLVVNDGSCVASLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPGKHVVRLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGFLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKSNLVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKDKVHFSENFFPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCADDLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATDQKSQHLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFDVVLPKGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKASN

Homology

[Close]

BLAST of Tan0012345 vs. ExPASy Swiss-Prot
Match: Q9SW95 (Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 PE=1 SV=2)

HSP 1 Score: 604.7 bits (1558), Expect = 1.1e-171
Identity = 332/628 (52.87%), Postives = 440/628 (70.06%), Query Frame = 0


Query: 11  PLTFSKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVREAPSPADATVRA--- 70
           P+T SKYSKRV LKT+LD +D G G    ++V+GGWVKS++ V + +P P    V A   
Sbjct: 20  PITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLPVVAAPSP 79

Query: 71  -AGEALPPRAKDVSCIELIQSRIPIVRSLLKMLYGNNLHGG------EKLETPVQK--PP 130
            +G        ++ C E+IQS++ I +    +L G    GG      +K E   QK  PP
Sbjct: 80  SSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSG----GGKTYPIFDKTELAGQKAVPP 139

Query: 131 LPSTAFLVVNDGSCVASLQVVIESSLH--HPGQLIPTGTCILVEGILKRAPV--PGKHVV 190
                + +++DGS ++SLQVV++S+L      QL+  GTCI+ EG+L R P+    KHV+
Sbjct: 140 PEYVFYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVL-RLPLAASAKHVI 199

Query: 191 RLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTF 250
            LE EK+LHVG V+ EKYPLS+K+LP+  LR FS+FRPRTTTV SV R+ +ALT A+HTF
Sbjct: 200 ELEAEKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTF 259

Query: 251 FQNHGFLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAA 310
            Q HGF YVQ+P ITTT   GF E F VTT+ GK+  K+ +    +E DG S++TVKA  
Sbjct: 260 LQYHGFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEEKKPPVQEKDGFSIDTVKAVI 319

Query: 311 IEKSNLVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKS--KKDKVHFSENF 370
            EK+ L+  L+RS+SNRE +VAAV DLKKTN+LA Q+E + K K+ +  K +K+ FS++F
Sbjct: 320 KEKTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDF 379

Query: 371 FPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELED 430
           F ++TYLT SG+ HLE+YA ALG VY+ GPRF ADKI++A+H AE W VE E+AFAEL+D
Sbjct: 380 FGRDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDD 439

Query: 431 VMNCADDLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILD 490
            M+CAD+  KFL K+VLE+  +DMKF+ KR+DKT   RLE+  +SS  + SYT  I +L 
Sbjct: 440 AMDCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQ 499

Query: 491 KATDQKSQHLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVA 550
           KAT  K +   K ++G ALT +HLSYL D IYK PVIV  YPK  K F VRLNDD +TVA
Sbjct: 500 KATTTKFE--TKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLNDDKKTVA 559

Query: 551 AFDVVLPKGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAF 610
           AFD+V+PK GVVI+GSQ EERF++L  R+ E G  RE++EWYL+++RHGTVKHSG SL+ 
Sbjct: 560 AFDLVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSM 619

Query: 611 DRFVLFTTGLTDIKDVIPFPRSFGKASN 621
           ++ +L+ TGL DIKD IPFPRS+GKA+N
Sbjct: 620 EQMLLYATGLPDIKDAIPFPRSWGKANN 638

[Close]

BLAST of Tan0012345 vs. ExPASy Swiss-Prot
Match: Q9SW96 (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 2.0e-131
Identity = 251/609 (41.22%), Postives = 370/609 (60.76%), Query Frame = 0


Query: 15  SKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVREAPSPADATVRAAGEALPP 74
           +++S RVL++T+LD  DGG G     + +GGWVKS ++  +                   
Sbjct: 27  AQFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRDQGKR------------------ 86

Query: 75  RAKDVSCIELIQSRIPIVRSLLKMLYGNNLHGGEKLETPVQKPPLPSTAFLVVNDGSCVA 134
                                                         + +FL VNDGSC A
Sbjct: 87  ----------------------------------------------TFSFLAVNDGSCPA 146

Query: 135 SLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPGK---HVVRLEVEKILHVGKVEQEK 194
           +LQV+++ SL+    L+ TGTC+ V+G+LK  P  GK     + L V K++ VG V+  K
Sbjct: 147 NLQVMVDPSLYDVSNLVATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDASK 206

Query: 195 YPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGFLYVQLPTITTT 254
           YPL + KL ++ LR   + R RT ++++V RIRNAL FATH+FFQ H FLY+  P ITT+
Sbjct: 207 YPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITTS 266

Query: 255 DSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKSNLVKELERSESNR 314
           D EG  E F  TT+   +   + +  ++       +E  +   IE+ N+V EL+ +++++
Sbjct: 267 DCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKASK 326

Query: 315 EALVAAVQDLKKTNELALQLEARGKLK-SKSKKD-KVHFSENFFPQETYLTVSGQLHLET 374
           EA+ AAV +LK   E    ++ R +L+    KKD  + +S++FF ++ +LTVSGQL +ET
Sbjct: 327 EAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQVET 386

Query: 375 YACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCADDLVKFLSKWVL 434
           YACAL NVY+ GP FRA+   +++H AE WMVE EIAFA+LED MNCA+  VK++  W+L
Sbjct: 387 YACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWLL 446

Query: 435 EHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATDQKSQHLKKLKFGA 494
           E C  DM+ + K  D   I RL+   ++ F +++YT AIE+L++A  +  +    +++G 
Sbjct: 447 EKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGKEFDNNVEWGI 506

Query: 495 ALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFDVVLPKGGVVISGSQ 554
            L ++H  YL + +++KP+IV+NYPK  K F +RLNDD +TVAA DV++PK G +I GSQ
Sbjct: 507 DLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQ 566

Query: 555 KEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVI 614
           +EER+DV+  R++EMGLP E YEWYL+++R+GTVKH GF L F+R +LF TGL +I+DVI
Sbjct: 567 REERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHCGFGLGFERMILFATGLDNIRDVI 570

Query: 615 PFPRSFGKA 619
           PFPR  GKA
Sbjct: 627 PFPRYPGKA 570

[Close]

BLAST of Tan0012345 vs. ExPASy Swiss-Prot
Match: Q9SSK1 (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 6.3e-122
Identity = 230/502 (45.82%), Postives = 333/502 (66.33%), Query Frame = 0


Query: 123 AFLVVNDGSCVASLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPG--KHVVRLEVEK 182
           AFL VNDGSC A+LQV+++SSL+   +L+ TGTC+ V+G+LK  P     K  + L VE 
Sbjct: 68  AFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGKGLKQSIELSVET 127

Query: 183 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGF 242
           ++ VG V+   YPL + KL  +FLR   + R RT  +++V RIRNAL FATH+FFQ H F
Sbjct: 128 VIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSF 187

Query: 243 LYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKSNL 302
           LY+  P ITT+D EG  E F VTT+   +   + +  ++       +E  +    E+   
Sbjct: 188 LYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGEA 247

Query: 303 VKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKD--KVHFSENFFPQETY 362
           V +L+ +++++E + A+V  L         +E R +LK    K+  K+ +S +FF ++ +
Sbjct: 248 VAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAF 307

Query: 363 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCAD 422
           LTVSGQL +ETYACAL +VY+ GP FRA+   +++H AE WMVE EIAFA++ D MNCA+
Sbjct: 308 LTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAE 367

Query: 423 DLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATDQ- 482
             VK++ KW+++ C DDM+ + K +D+    RL     +SF++V+YT AIE L+KA  Q 
Sbjct: 368 AYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQG 427

Query: 483 KSQHLKKLKFGAALTADHLSYLADAIY-KKPVIVFNYPKETKPFNVRLNDDGQTVAAFDV 542
           K     K+++G  L ++H  YL +  + +KP+IV+NYPK  K F +RLNDD +TVAA DV
Sbjct: 428 KVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDV 487

Query: 543 VLPKGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFV 602
           ++PK G +I GSQ+EER+DV+  R++EMGLP E YEWYL+++R+GTVKH GF L F+R +
Sbjct: 488 LVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKHCGFGLGFERMI 547

Query: 603 LFTTGLTDIKDVIPFPRSFGKA 619
            F TG+ +I+DVIPFPR  GKA
Sbjct: 548 QFATGIDNIRDVIPFPRYPGKA 569

[Close]

BLAST of Tan0012345 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.2e-96
Identity = 194/498 (38.96%), Postives = 294/498 (59.04%), Query Frame = 0


Query: 124 FLVVNDGSCVASLQVVIESSLHHPGQLI---PTGTCILVEGILKRAPVPGKHVVRLEVEK 183
           F+ +NDGS +++ Q++    +    QLI    TG  +   G +  +P   ++ + ++   
Sbjct: 40  FIEINDGSTLSNFQIIATPDIAGYDQLINQLSTGVSVSAIGTIVESPGKEQN-LEMQATA 99

Query: 184 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGF 243
           I  +GK + E Y L +K+   +FLR  ++ RPRT T+ +V R+RNAL FATH FFQ  GF
Sbjct: 100 ITIIGKCDPEVYLLQKKRHTFEFLRSIAHLRPRTNTIGAVTRVRNALAFATHQFFQKRGF 159

Query: 244 LYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKSNL 303
           LY+  P IT +D EG  + F VTT                              ++++N 
Sbjct: 160 LYIHTPIITGSDCEGAGKMFQVTT------------------------------LDQNN- 219

Query: 304 VKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKDKVHFSENFFPQETYLT 363
                                                 +++ + +V ++++FF + TYLT
Sbjct: 220 -------------------------------------PARTPEGRVDYTQDFFGKPTYLT 279

Query: 364 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCADDL 423
           VSGQL+ E YACAL +VY+ GP FRA+   +++H AE WM+E E+AFA+L D M+CA+D 
Sbjct: 280 VSGQLNGEIYACALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEMAFADLNDNMDCAEDY 339

Query: 424 VKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATDQKSQ 483
           +K++ K+VL++C +DM+F  K +    I RLE  I +SFE+ SYT A+ IL+KA D+K +
Sbjct: 340 LKYILKYVLDNCQEDMEFFNKHVATDLISRLEHVINTSFERASYTYAVRILEKA-DKKFE 399

Query: 484 HLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFDVVLPK 543
           +   +K+G  L ++H  +LA+  + KPVI+ +YPK+ K F +R N+D +TVAA DV++PK
Sbjct: 400 Y--PVKWGLDLQSEHERFLAEEFFGKPVILTDYPKDIKAFYMRTNEDNKTVAAMDVLVPK 459

Query: 544 GGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTT 603
            G +I GSQ+EER  VL +++KE  LP E+Y WYLE+++ G+V HSGF   F+R V FTT
Sbjct: 460 VGEIIGGSQREERLSVLESKLKEFNLPAEEYWWYLELRKFGSVPHSGFGAGFERLVQFTT 465

Query: 604 GLTDIKDVIPFPRSFGKA 619
           G+ +I+DVIPFPR  GKA
Sbjct: 520 GMENIRDVIPFPRHPGKA 465

[Close]

BLAST of Tan0012345 vs. ExPASy Swiss-Prot
Match: P52276 (Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=asnS PE=3 SV=2)

HSP 1 Score: 350.9 bits (899), Expect = 2.9e-95
Identity = 199/503 (39.56%), Postives = 290/503 (57.65%), Query Frame = 0


Query: 116 KPPLPSTAFLVVNDGSCVASLQVVIESSLHHPGQL---IPTGTCILVEGILKRAPVPGK- 175
           K  L   +F+ VNDGS +A+LQVV++ SL    +L   + TG  ++VEG  K AP PGK 
Sbjct: 28  KRTLKDFSFVEVNDGSSLANLQVVLDGSLADYDRLLSQLQTGAALVVEG--KLAPSPGKG 87

Query: 176 HVVRLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFAT 235
             V L+  K+  +G  +   YPL +K+   +FLR   + RPRT T+ +VMR+RNA   A 
Sbjct: 88  QRVELKATKLELLGGADPGSYPLQKKRHSFEFLRTIGHLRPRTNTIGAVMRVRNACATAI 147

Query: 236 HTFFQNHGFLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVK 295
           H FFQ  GFL+V  P IT +D EG  + F VTT+                          
Sbjct: 148 HQFFQERGFLWVHTPIITASDCEGAGDLFKVTTL-------------------------- 207

Query: 296 AAAIEKSNLVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKDKVHFSEN 355
                      +L+R   N E                                 + +S++
Sbjct: 208 -----------DLQRVPKNGEG--------------------------------IDYSQD 267

Query: 356 FFPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELE 415
           FF ++ YLTVSGQL  E  A A  NVY+ GP FRA+   +++H AE WMVE E+AF +LE
Sbjct: 268 FFGKQAYLTVSGQLEAEVMALAFQNVYTFGPTFRAENSNTSRHLAEFWMVEPEMAFCDLE 327

Query: 416 DVMNCADDLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEIL 475
                A++ +K++ K+VLE C +DM+F  +RID T +   ++ I + F  ++Y+ AI++L
Sbjct: 328 GDRQWAEEFLKYIFKFVLEKCPEDMEFFDQRIDNTVLATADNIINNEFAWLTYSEAIKLL 387

Query: 476 DKATDQKSQHLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTV 535
           +KA DQK ++   + +G  L ++H  YLA+ ++K+P IV +YPK+ K F +RLNDDG+TV
Sbjct: 388 EKA-DQKFEY--PVAWGVDLQSEHERYLAETVFKRPTIVTDYPKDIKAFYMRLNDDGKTV 447

Query: 536 AAFDVVLPKGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLA 595
           AA D++ PK G +I GSQ+EER D+L  RM+E G+P E   WYL+++R+G+V H+GF L 
Sbjct: 448 AAMDILAPKIGEIIGGSQREERLDILTQRMQEQGVPEEDLWWYLDLRRYGSVPHAGFGLG 456

Query: 596 FDRFVLFTTGLTDIKDVIPFPRS 615
           F+R V F TG+ +I+DVIPFPR+
Sbjct: 508 FERIVQFMTGMANIRDVIPFPRT 456

[Close]

BLAST of Tan0012345 vs. NCBI nr
Match: XP_023004185.1 (asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita maxima])

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 562/622 (90.35%), Postives = 591/622 (95.02%), Query Frame = 0


Query: 1   MAAQHPPLFTPLTFSKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVREAPSP 60
           MAA+HP  FTP+TFSKYSKRVLLKTVLD +DGGLGFVD KLV+GGWVKSSKEV REA  P
Sbjct: 1   MAAEHPTAFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60

Query: 61  ADATVRAAGEALPPRAKDVSCIELIQSRIPIVRSLLKMLYGNNLHGGEKLETPVQK--PP 120
           ADA+VR AG+ALP RAKDVSCIE++QSRIPIVRSLL+MLYGN+LH GEKLETPV K  PP
Sbjct: 61  ADASVRTAGDALPRRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETPVHKPPPP 120

Query: 121 LPSTAFLVVNDGSCVASLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPGKHVVRLEV 180
           LPST FLVVNDGSCVASLQVV+ESSLHHPGQL+PTGTCILVEGILKRAP PGKH+VRLEV
Sbjct: 121 LPSTTFLVVNDGSCVASLQVVVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180

Query: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNH 240
           EKILHVGKVEQEKYPL+QKKLPMD LRKFSYFRPRTTTVASVMRI NALTFATHTFFQNH
Sbjct: 181 EKILHVGKVEQEKYPLTQKKLPMDLLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQNH 240

Query: 241 GFLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKS 300
           GFLYVQLPTITTTDSEGFSEKFHVTT AGKS  KK+EPTEDKETDGVSLETVKAAA+EK 
Sbjct: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGKSEDKKIEPTEDKETDGVSLETVKAAAVEKR 300

Query: 301 NLVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKDKVHFSENFFPQETY 360
           N+VKELERSESNREAL AAVQDLKKTNELALQLEARGK KS+ KKDKVH SENFFPQETY
Sbjct: 301 NIVKELERSESNREALAAAVQDLKKTNELALQLEARGKQKSQLKKDKVHISENFFPQETY 360

Query: 361 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCAD 420
           LTVSGQLHLETYACALGNVYSCGPRFRAD+I SAKHAAEMWMVEVEIAFAELEDV+NCAD
Sbjct: 361 LTVSGQLHLETYACALGNVYSCGPRFRADRIVSAKHAAEMWMVEVEIAFAELEDVINCAD 420

Query: 421 DLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATDQK 480
           DLVKFLSKWVLEHCLDDMKFVQKRIDKTS+HRLES ITSSFEKVSYTAA+EILDKATDQK
Sbjct: 421 DLVKFLSKWVLEHCLDDMKFVQKRIDKTSVHRLESTITSSFEKVSYTAAVEILDKATDQK 480

Query: 481 SQHLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFDVVL 540
           SQ LK+LKFGAALT DHLSYLADAI+KKPVIV+NYPKETKPFNVRLNDDG+TVAAFDVVL
Sbjct: 481 SQRLKRLKFGAALTDDHLSYLADAIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVL 540

Query: 541 PKGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF 600
           PKGGVVISGSQKEERFDVL++RMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF
Sbjct: 541 PKGGVVISGSQKEERFDVLLSRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF 600

Query: 601 TTGLTDIKDVIPFPRSFGKASN 621
           TTGLTDIKDVIPFPRS GK S+
Sbjct: 601 TTGLTDIKDVIPFPRSLGKVSH 622

[Close]

BLAST of Tan0012345 vs. NCBI nr
Match: XP_023514428.1 (asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 561/622 (90.19%), Postives = 589/622 (94.69%), Query Frame = 0


Query: 1   MAAQHPPLFTPLTFSKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVREAPSP 60
           MAA+HP +F P+TFSKYSKRVLLKTVLD +DGGLGFVD KLV+GGWVKSSKEV REA  P
Sbjct: 1   MAAEHPTVFAPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAVVP 60

Query: 61  ADATVRAAGEALPPRAKDVSCIELIQSRIPIVRSLLKMLYGNNLHGGEKLETPVQK--PP 120
           ADA+VR AG+ALPPRAKDVSCIE++QSRIPIVRSLL+MLYGN+LH GEKLET V K  PP
Sbjct: 61  ADASVRTAGDALPPRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETSVHKPPPP 120

Query: 121 LPSTAFLVVNDGSCVASLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPGKHVVRLEV 180
           LPST FLVVNDGSCVASLQVV+ESSLHHPGQL+PTGTCILVEGILKR P PGKH+VRLEV
Sbjct: 121 LPSTTFLVVNDGSCVASLQVVVESSLHHPGQLMPTGTCILVEGILKRTPAPGKHLVRLEV 180

Query: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNH 240
           EKILHVGKVEQEKYPL+QKKLPMD LRKFSYFRPRTTTVASVMR+RNALTFATHTFFQNH
Sbjct: 181 EKILHVGKVEQEKYPLTQKKLPMDLLRKFSYFRPRTTTVASVMRVRNALTFATHTFFQNH 240

Query: 241 GFLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKS 300
           GFLYVQLPTITTTDSEGFSEKFHVTT AGKS  KK+EPTEDKETDGVSLETVKAAA+EK 
Sbjct: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGKSEDKKIEPTEDKETDGVSLETVKAAAVEKR 300

Query: 301 NLVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKDKVHFSENFFPQETY 360
           N+VKELERSESNREAL AAVQDLKKTNELALQLEARGK K K KKDKVH SE FFPQETY
Sbjct: 301 NIVKELERSESNREALAAAVQDLKKTNELALQLEARGKQKLKLKKDKVHVSETFFPQETY 360

Query: 361 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCAD 420
           LTVSGQLHLETYACALGNVYSCGPRFRAD+IESAKHAAEMWMVEVEIAFAELEDV+NCAD
Sbjct: 361 LTVSGQLHLETYACALGNVYSCGPRFRADRIESAKHAAEMWMVEVEIAFAELEDVINCAD 420

Query: 421 DLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATDQK 480
           DLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLES ITSSFE+VSYTAAIEILDKATDQK
Sbjct: 421 DLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESTITSSFERVSYTAAIEILDKATDQK 480

Query: 481 SQHLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFDVVL 540
           SQ LK+LKFGAALT DHLSYLADAI+KKPVIV+NYPKETKPFNVRLNDDG+TVAAFDVVL
Sbjct: 481 SQRLKRLKFGAALTDDHLSYLADAIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVL 540

Query: 541 PKGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF 600
           PKGGVVISGSQKEER DVL+TRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF
Sbjct: 541 PKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF 600

Query: 601 TTGLTDIKDVIPFPRSFGKASN 621
           TTGLTDIKDVIPFPRS GK S+
Sbjct: 601 TTGLTDIKDVIPFPRSLGKVSH 622

[Close]

BLAST of Tan0012345 vs. NCBI nr
Match: XP_038899280.1 (asparagine--tRNA ligase, cytoplasmic 2 [Benincasa hispida])

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 558/621 (89.86%), Postives = 585/621 (94.20%), Query Frame = 0


Query: 1   MAAQHPPLFTPLTFSKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVRE-APS 60
           MAA+HP +FTP+TFSKYS RVLLKT+LD+ DGGL FV  KLV+GGWVKSSKEV+RE APS
Sbjct: 1   MAAEHPTVFTPVTFSKYSNRVLLKTLLDTADGGLAFVGQKLVIGGWVKSSKEVIREAAPS 60

Query: 61  PADATVRAAGEALPPRAKDVSCIELIQSRIPIVRSLLKMLYGNNLHGGEKLETPVQKPPL 120
           PADA+VRA   ALPP+AKDVSCIE+IQSRIPIVRSLLKMLYGNNLHGGEKLET V KPPL
Sbjct: 61  PADASVRAVANALPPKAKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLETAVPKPPL 120

Query: 121 PSTAFLVVNDGSCVASLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPGKHVVRLEVE 180
           PSTAFL VNDGSCVASLQVV+ESSLHHP  L+PTGTCILVEGILK+ PVPGKHVVRLEVE
Sbjct: 121 PSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKQVPVPGKHVVRLEVE 180

Query: 181 KILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHG 240
           KILHVGKVEQ+KYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRI NALTFATHTFFQNH 
Sbjct: 181 KILHVGKVEQQKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQNHS 240

Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKSN 300
           FLYVQLPTITTTDSEGFSEKFHVTT+AGK  GKKVEPTEDKE DGVSLETVKAA  EKSN
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFHVTTLAGK--GKKVEPTEDKEHDGVSLETVKAAITEKSN 300

Query: 301 LVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKDKVHFSENFFPQETYL 360
           LVKELERSESN+EALVAAVQDLKKTNELALQLEA+ K K+KS+KDKV    NFFPQETYL
Sbjct: 301 LVKELERSESNKEALVAAVQDLKKTNELALQLEAKEKSKTKSRKDKV-IPGNFFPQETYL 360

Query: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCADD 420
           T SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELE+ MNCADD
Sbjct: 361 TASGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELENAMNCADD 420

Query: 421 LVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATDQKS 480
           LVKFLSKWVL+HCLDDMKFVQKRIDKTSIHRLES I+ SFEKVSY AAIEIL+KATDQKS
Sbjct: 421 LVKFLSKWVLDHCLDDMKFVQKRIDKTSIHRLESVISCSFEKVSYIAAIEILEKATDQKS 480

Query: 481 QHLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFDVVLP 540
           QHLKKLKFGAALT DHLSYLADAI+KKPVIV+NYPKETKPFNVRLNDDG+TVAAFDVVLP
Sbjct: 481 QHLKKLKFGAALTVDHLSYLADAIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLP 540

Query: 541 KGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFT 600
           KGGVVISGSQKEERFDVL+TRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFT
Sbjct: 541 KGGVVISGSQKEERFDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFT 600

Query: 601 TGLTDIKDVIPFPRSFGKASN 621
           TGLTDIKDVIPFPRSFGK SN
Sbjct: 601 TGLTDIKDVIPFPRSFGKVSN 618

[Close]

BLAST of Tan0012345 vs. NCBI nr
Match: XP_022959835.1 (asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita moschata])

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 554/622 (89.07%), Postives = 584/622 (93.89%), Query Frame = 0


Query: 1   MAAQHPPLFTPLTFSKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVREAPSP 60
           MAA+HP +FTP+TFSKYSKRVLLKTVLD +DGGLGFVD KLV+GGWVKSSKEV REA  P
Sbjct: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60

Query: 61  ADATVRAAGEALPPRAKDVSCIELIQSRIPIVRSLLKMLYGNNLHGGEKLETPVQK--PP 120
           ADA+VR A +ALPPRAKDVSCIE++QSRIPIVRSLL+MLYGN+LH GEKLET V K  PP
Sbjct: 61  ADASVRTACDALPPRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETSVHKPPPP 120

Query: 121 LPSTAFLVVNDGSCVASLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPGKHVVRLEV 180
           LPST FLVVNDGSCVASLQV +ESSLHHPGQL+PTGTCILVEGILKRAP PGKH+VRLEV
Sbjct: 121 LPSTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180

Query: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNH 240
           EKILHVGKVEQEKYPL+QKKL +D LRKFSYFRPRTTTVA+VMR+ NALTFATHTFFQNH
Sbjct: 181 EKILHVGKVEQEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNH 240

Query: 241 GFLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKS 300
           GFLYVQLPTITTTDSEGFSEKFHVTT AG S  KK+E TEDKETDGV+LETVKAAAIEK 
Sbjct: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVKKIESTEDKETDGVNLETVKAAAIEKK 300

Query: 301 NLVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKDKVHFSENFFPQETY 360
           N+VKELERSESNREAL AAVQDLKKTNELALQLEARGK KSK KKDKVH SENFFPQETY
Sbjct: 301 NIVKELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKDKVHVSENFFPQETY 360

Query: 361 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCAD 420
           LTVSGQLHLETYACALGNVYSCGPRFRAD+IESAKHAAEMWMVEVEIAFAELEDV+NCAD
Sbjct: 361 LTVSGQLHLETYACALGNVYSCGPRFRADRIESAKHAAEMWMVEVEIAFAELEDVINCAD 420

Query: 421 DLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATDQK 480
           DLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLES ITSSFEKVSYTAAIEILDKATDQK
Sbjct: 421 DLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESTITSSFEKVSYTAAIEILDKATDQK 480

Query: 481 SQHLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFDVVL 540
           SQ  K+LKFGAALT DHLSYLADA +KKPVIV+NYPKETKPFNVRLNDDG+TVAAFDVVL
Sbjct: 481 SQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVL 540

Query: 541 PKGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF 600
           PKGGVVISGSQKEER DVL+TRMKEMGLPREQYEWYLEI+RHGTVKHSGFSLAFDRFVLF
Sbjct: 541 PKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRRHGTVKHSGFSLAFDRFVLF 600

Query: 601 TTGLTDIKDVIPFPRSFGKASN 621
           TTGLTDIKD IPFPRS GK S+
Sbjct: 601 TTGLTDIKDAIPFPRSLGKVSH 622

[Close]

BLAST of Tan0012345 vs. NCBI nr
Match: KAG6593030.1 (Asparagine--tRNA ligase, cytoplasmic 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 547/624 (87.66%), Postives = 579/624 (92.79%), Query Frame = 0


Query: 1   MAAQHPPLFTPLTFSKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVREAPSP 60
           MAA+HP +FTP+TFSKYSKRVLLKTVLD +DGGLGFVD KLV+GGWVKSSKEV REA  P
Sbjct: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60

Query: 61  ADATVRAAGEALPPRAKDVSCIELIQSRIPIVRSLLKMLYGNNLHGGEKLETPVQK---- 120
           ADA+VR AG+ALPPRAKDVSCIE++QSRIPIVRSLL+MLYGN+LH GEKLET V K    
Sbjct: 61  ADASVRTAGDALPPRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETSVHKPPPP 120

Query: 121 PPLPSTAFLVVNDGSCVASLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPGKHVVRL 180
           PP P T FLVVNDGSCVASLQV +ESSLHHPGQL+PTGTCILVEGILKRAP PGKH+VRL
Sbjct: 121 PPPPPTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRL 180

Query: 181 EVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQ 240
           EVEKILHVGKVE EKYPL+QKKL +DFLRKFSYFRPRTTTVA+VMR+ NALTFATHTFFQ
Sbjct: 181 EVEKILHVGKVEPEKYPLTQKKLLIDFLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQ 240

Query: 241 NHGFLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIE 300
           NHGFLYVQLPTITTTDSEGFSEKFHVTT AG S  +K+E TEDKETDGVSLETVKAAAIE
Sbjct: 241 NHGFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIE 300

Query: 301 KSNLVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKDKVHFSENFFPQE 360
           K N+V ELERSESNREAL AAVQDLKKTNE+ALQLEARGK KSK KKDKVH SENFFPQE
Sbjct: 301 KKNIVNELERSESNREALAAAVQDLKKTNEIALQLEARGKQKSKLKKDKVHVSENFFPQE 360

Query: 361 TYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNC 420
           TYLTVSGQLHLETYACALGNVYSCGPRFRAD+IESAKHAAEMWMVEVEIAFAELEDV+NC
Sbjct: 361 TYLTVSGQLHLETYACALGNVYSCGPRFRADRIESAKHAAEMWMVEVEIAFAELEDVINC 420

Query: 421 ADDLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATD 480
           ADDLVKFLSKWV EHCLDDMKFVQKRIDKTSIH LES I SSFEKVSYTAAI+ILDKATD
Sbjct: 421 ADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAAIKILDKATD 480

Query: 481 QKSQHLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFDV 540
           QKSQ LK+LKFGAALT DHLSYLAD  +KKPVIV+NYPKETKPFNVRLNDDG+TVAAFDV
Sbjct: 481 QKSQCLKRLKFGAALTDDHLSYLADVFFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDV 540

Query: 541 VLPKGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFV 600
           VLPKGGVVISGSQKEER DVL+TRMKEMGLPREQYEWYLEI+RHGTVKHSGFSLAFDRFV
Sbjct: 541 VLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRRHGTVKHSGFSLAFDRFV 600

Query: 601 LFTTGLTDIKDVIPFPRSFGKASN 621
           LFTTGLTDIKD IPFPRS GK S+
Sbjct: 601 LFTTGLTDIKDAIPFPRSLGKVSH 624

[Close]

BLAST of Tan0012345 vs. ExPASy TrEMBL
Match: A0A6J1KTV9 (asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497586 PE=4 SV=1)

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 562/622 (90.35%), Postives = 591/622 (95.02%), Query Frame = 0


Query: 1   MAAQHPPLFTPLTFSKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVREAPSP 60
           MAA+HP  FTP+TFSKYSKRVLLKTVLD +DGGLGFVD KLV+GGWVKSSKEV REA  P
Sbjct: 1   MAAEHPTAFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60

Query: 61  ADATVRAAGEALPPRAKDVSCIELIQSRIPIVRSLLKMLYGNNLHGGEKLETPVQK--PP 120
           ADA+VR AG+ALP RAKDVSCIE++QSRIPIVRSLL+MLYGN+LH GEKLETPV K  PP
Sbjct: 61  ADASVRTAGDALPRRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETPVHKPPPP 120

Query: 121 LPSTAFLVVNDGSCVASLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPGKHVVRLEV 180
           LPST FLVVNDGSCVASLQVV+ESSLHHPGQL+PTGTCILVEGILKRAP PGKH+VRLEV
Sbjct: 121 LPSTTFLVVNDGSCVASLQVVVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180

Query: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNH 240
           EKILHVGKVEQEKYPL+QKKLPMD LRKFSYFRPRTTTVASVMRI NALTFATHTFFQNH
Sbjct: 181 EKILHVGKVEQEKYPLTQKKLPMDLLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQNH 240

Query: 241 GFLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKS 300
           GFLYVQLPTITTTDSEGFSEKFHVTT AGKS  KK+EPTEDKETDGVSLETVKAAA+EK 
Sbjct: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGKSEDKKIEPTEDKETDGVSLETVKAAAVEKR 300

Query: 301 NLVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKDKVHFSENFFPQETY 360
           N+VKELERSESNREAL AAVQDLKKTNELALQLEARGK KS+ KKDKVH SENFFPQETY
Sbjct: 301 NIVKELERSESNREALAAAVQDLKKTNELALQLEARGKQKSQLKKDKVHISENFFPQETY 360

Query: 361 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCAD 420
           LTVSGQLHLETYACALGNVYSCGPRFRAD+I SAKHAAEMWMVEVEIAFAELEDV+NCAD
Sbjct: 361 LTVSGQLHLETYACALGNVYSCGPRFRADRIVSAKHAAEMWMVEVEIAFAELEDVINCAD 420

Query: 421 DLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATDQK 480
           DLVKFLSKWVLEHCLDDMKFVQKRIDKTS+HRLES ITSSFEKVSYTAA+EILDKATDQK
Sbjct: 421 DLVKFLSKWVLEHCLDDMKFVQKRIDKTSVHRLESTITSSFEKVSYTAAVEILDKATDQK 480

Query: 481 SQHLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFDVVL 540
           SQ LK+LKFGAALT DHLSYLADAI+KKPVIV+NYPKETKPFNVRLNDDG+TVAAFDVVL
Sbjct: 481 SQRLKRLKFGAALTDDHLSYLADAIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVL 540

Query: 541 PKGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF 600
           PKGGVVISGSQKEERFDVL++RMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF
Sbjct: 541 PKGGVVISGSQKEERFDVLLSRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF 600

Query: 601 TTGLTDIKDVIPFPRSFGKASN 621
           TTGLTDIKDVIPFPRS GK S+
Sbjct: 601 TTGLTDIKDVIPFPRSLGKVSH 622

[Close]

BLAST of Tan0012345 vs. ExPASy TrEMBL
Match: A0A6J1H7G6 (asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460779 PE=4 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 554/622 (89.07%), Postives = 584/622 (93.89%), Query Frame = 0


Query: 1   MAAQHPPLFTPLTFSKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVREAPSP 60
           MAA+HP +FTP+TFSKYSKRVLLKTVLD +DGGLGFVD KLV+GGWVKSSKEV REA  P
Sbjct: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60

Query: 61  ADATVRAAGEALPPRAKDVSCIELIQSRIPIVRSLLKMLYGNNLHGGEKLETPVQK--PP 120
           ADA+VR A +ALPPRAKDVSCIE++QSRIPIVRSLL+MLYGN+LH GEKLET V K  PP
Sbjct: 61  ADASVRTACDALPPRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETSVHKPPPP 120

Query: 121 LPSTAFLVVNDGSCVASLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPGKHVVRLEV 180
           LPST FLVVNDGSCVASLQV +ESSLHHPGQL+PTGTCILVEGILKRAP PGKH+VRLEV
Sbjct: 121 LPSTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180

Query: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNH 240
           EKILHVGKVEQEKYPL+QKKL +D LRKFSYFRPRTTTVA+VMR+ NALTFATHTFFQNH
Sbjct: 181 EKILHVGKVEQEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNH 240

Query: 241 GFLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKS 300
           GFLYVQLPTITTTDSEGFSEKFHVTT AG S  KK+E TEDKETDGV+LETVKAAAIEK 
Sbjct: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVKKIESTEDKETDGVNLETVKAAAIEKK 300

Query: 301 NLVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKDKVHFSENFFPQETY 360
           N+VKELERSESNREAL AAVQDLKKTNELALQLEARGK KSK KKDKVH SENFFPQETY
Sbjct: 301 NIVKELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKDKVHVSENFFPQETY 360

Query: 361 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCAD 420
           LTVSGQLHLETYACALGNVYSCGPRFRAD+IESAKHAAEMWMVEVEIAFAELEDV+NCAD
Sbjct: 361 LTVSGQLHLETYACALGNVYSCGPRFRADRIESAKHAAEMWMVEVEIAFAELEDVINCAD 420

Query: 421 DLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATDQK 480
           DLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLES ITSSFEKVSYTAAIEILDKATDQK
Sbjct: 421 DLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESTITSSFEKVSYTAAIEILDKATDQK 480

Query: 481 SQHLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFDVVL 540
           SQ  K+LKFGAALT DHLSYLADA +KKPVIV+NYPKETKPFNVRLNDDG+TVAAFDVVL
Sbjct: 481 SQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVL 540

Query: 541 PKGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF 600
           PKGGVVISGSQKEER DVL+TRMKEMGLPREQYEWYLEI+RHGTVKHSGFSLAFDRFVLF
Sbjct: 541 PKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRRHGTVKHSGFSLAFDRFVLF 600

Query: 601 TTGLTDIKDVIPFPRSFGKASN 621
           TTGLTDIKD IPFPRS GK S+
Sbjct: 601 TTGLTDIKDAIPFPRSLGKVSH 622

[Close]

BLAST of Tan0012345 vs. ExPASy TrEMBL
Match: A0A5D3BD07 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1970G00300 PE=3 SV=1)

HSP 1 Score: 1068.1 bits (2761), Expect = 1.3e-308
Identity = 546/621 (87.92%), Postives = 578/621 (93.08%), Query Frame = 0


Query: 1   MAAQHPPLFTPLTFSKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVREAPSP 60
           MAAQ P +F+P+TFSKYS RVLLKT+LD+TDGGL FV  KLVVGGWVKSSKEV+R+AP P
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAP-P 60

Query: 61  ADATVRAAGEALPPRA-KDVSCIELIQSRIPIVRSLLKMLYGNNLHGGEKLETPVQKPPL 120
           A A   A  ++LPP+  KDVSCIE+IQSRIPIVRSLLKMLYGNNLHGGEKL+TPV KPPL
Sbjct: 61  APAPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPL 120

Query: 121 PSTAFLVVNDGSCVASLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPGKHVVRLEVE 180
           PSTAFL +NDGSCVASLQVV+ESSLHHP  L+PTGTCILVEG+LK+ PVPGKHVVRLEVE
Sbjct: 121 PSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVE 180

Query: 181 KILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHG 240
           KILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+ 
Sbjct: 181 KILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNS 240

Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKSN 300
           FLYVQLPTITTTDSEGFSEKFHVTT+AGK  GK+VE TEDKE DG+SLETVKAA  EKSN
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFHVTTLAGK--GKRVESTEDKEPDGLSLETVKAAITEKSN 300

Query: 301 LVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKDKVHFSENFFPQETYL 360
           LVKELERSESNREALVAAVQDLKKTN+LALQLEAR K KSKS+KDKV    NF PQETYL
Sbjct: 301 LVKELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKV-IPGNFLPQETYL 360

Query: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCADD 420
           TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAEL+D MNCADD
Sbjct: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADD 420

Query: 421 LVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATDQKS 480
           LVKFLSKWVLEHC+DDMKFVQKRIDKTSIHRLES I+ SFEKVSYTAAIEIL+KATDQKS
Sbjct: 421 LVKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKS 480

Query: 481 QHLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFDVVLP 540
           QHLKKLKFGAALTADHLSYLAD I+KKPVIV+NYPKETKPFNVRLNDDG+TVAAFDVVLP
Sbjct: 481 QHLKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLP 540

Query: 541 KGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFT 600
           KGGVVISGSQKEER DVL+TRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFT
Sbjct: 541 KGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFT 600

Query: 601 TGLTDIKDVIPFPRSFGKASN 621
           TGLTDIKDVIPFPRSFGK  N
Sbjct: 601 TGLTDIKDVIPFPRSFGKVIN 617

[Close]

BLAST of Tan0012345 vs. ExPASy TrEMBL
Match: A0A1S3C6Z5 (Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1)

HSP 1 Score: 1063.1 bits (2748), Expect = 4.3e-307
Identity = 546/621 (87.92%), Postives = 577/621 (92.91%), Query Frame = 0


Query: 1   MAAQHPPLFTPLTFSKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVREAPSP 60
           MAAQ P +F+P+TFSKYS RVLLKT+LD+TDGGL FV  KLVVGGWVKSSKEV+R+AP P
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAP-P 60

Query: 61  ADATVRAAGEALPPRA-KDVSCIELIQSRIPIVRSLLKMLYGNNLHGGEKLETPVQKPPL 120
           A     A  ++LPP+  KDVSCIE+IQSRIPIVRSLLKMLYGNNLHGGEKL+TPV KPPL
Sbjct: 61  AP----APADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPL 120

Query: 121 PSTAFLVVNDGSCVASLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPGKHVVRLEVE 180
           PSTAFL +NDGSCVASLQVV+ESSLHHP  L+PTGTCILVEG+LK+ PVPGKHVVRLEVE
Sbjct: 121 PSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVE 180

Query: 181 KILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHG 240
           KILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+ 
Sbjct: 181 KILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNS 240

Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKSN 300
           FLYVQLPTITTTDSEGFSEKFHVTT+AGK  GK+VE TEDKE DG+SLETVKAA  EKSN
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFHVTTLAGK--GKRVESTEDKEPDGLSLETVKAAITEKSN 300

Query: 301 LVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKDKVHFSENFFPQETYL 360
           LVKELERSESNREALVAAVQDLKKTNELALQLEAR K KSKS+KDKV    NF PQETYL
Sbjct: 301 LVKELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKV-IPGNFLPQETYL 360

Query: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCADD 420
           TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAEL+D MNCADD
Sbjct: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADD 420

Query: 421 LVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATDQKS 480
           LVKFLSKWVLEHC+DDMKFVQKRIDKTSIHRLES I+ SFEKVSYTAAIEIL+KATDQKS
Sbjct: 421 LVKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKS 480

Query: 481 QHLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFDVVLP 540
           QHLKKLKFGAALTADHLSYLAD I+KKPVIV+NYPKETKPFNVRLNDDG+TVAAFDVVLP
Sbjct: 481 QHLKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLP 540

Query: 541 KGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFT 600
           KGGVVISGSQKEER DVL+TRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFT
Sbjct: 541 KGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFT 600

Query: 601 TGLTDIKDVIPFPRSFGKASN 621
           TGLTDIKDVIPFPRSFGK  N
Sbjct: 601 TGLTDIKDVIPFPRSFGKVIN 613

[Close]

BLAST of Tan0012345 vs. ExPASy TrEMBL
Match: A0A6J1GR04 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456649 PE=3 SV=1)

HSP 1 Score: 1055.8 bits (2729), Expect = 6.9e-305
Identity = 535/622 (86.01%), Postives = 576/622 (92.60%), Query Frame = 0


Query: 1   MAAQHPPLFTPLTFSKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVREAPSP 60
           MAAQ P +FTP+TFSKY+KRVLL+TV +S DGGLGFVD KLVVGGWVKSSKEV+ +A  P
Sbjct: 1   MAAQQPTVFTPVTFSKYTKRVLLRTVFESGDGGLGFVDRKLVVGGWVKSSKEVIGDAVRP 60

Query: 61  -ADATVRAAGEALPPRAKDVSCIELIQSRIPIVRSLLKMLYGNNLHGGEKLETPVQKPPL 120
             DA+V+AAG+ LP +AKD SCIE++QSRIPIVRSLL+MLYGNNLHGGEKLET V +PPL
Sbjct: 61  LVDASVKAAGDVLPLKAKDASCIEIVQSRIPIVRSLLRMLYGNNLHGGEKLETNVHEPPL 120

Query: 121 PSTAFLVVNDGSCVASLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPGKHVVRLEVE 180
           PSTAFLVVNDGSCVA LQVV+ESSLH P  L+P+GTCILVEGILKR PVPGKH VRLEVE
Sbjct: 121 PSTAFLVVNDGSCVADLQVVVESSLHQPIHLMPSGTCILVEGILKRIPVPGKHTVRLEVE 180

Query: 181 KILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHG 240
           KILHVGKVE+EKYPLSQK++PMD LRKFSYFRPRT+TVASVMRIRNALTFATHTFFQNH 
Sbjct: 181 KILHVGKVEKEKYPLSQKRIPMDVLRKFSYFRPRTSTVASVMRIRNALTFATHTFFQNHS 240

Query: 241 FLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKSN 300
           FLYVQLPTITTTDSEGFSEKFHVTT+A K AGK+VEPTEDKETDGVSLETVKAA +EKSN
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFHVTTLATKLAGKRVEPTEDKETDGVSLETVKAAVMEKSN 300

Query: 301 LVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKDKVHFSENFFPQETYL 360
           LVKELERSE N+EAL AAVQDLK+TNELALQLEARGK K +S KDKV+F ENFFPQETYL
Sbjct: 301 LVKELERSEGNKEALAAAVQDLKRTNELALQLEARGKSKPESSKDKVNFHENFFPQETYL 360

Query: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCADD 420
           TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELED MNCADD
Sbjct: 361 TVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDAMNCADD 420

Query: 421 LVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKAT-DQK 480
           LVKFLSKWVL+HC+DDMKFV+KRIDKTSI RLESFI+ SFEKVSYTAAIE L+KAT DQK
Sbjct: 421 LVKFLSKWVLDHCVDDMKFVEKRIDKTSIPRLESFISCSFEKVSYTAAIESLEKATPDQK 480

Query: 481 SQHLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFDVVL 540
            QHLK LKFGAALTADHLSYLAD IYKKPVIV+NYP+ TKPFNVRLNDDG+TVAAFDVVL
Sbjct: 481 HQHLKSLKFGAALTADHLSYLADTIYKKPVIVYNYPEGTKPFNVRLNDDGKTVAAFDVVL 540

Query: 541 PKGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF 600
           PKGGVVISGSQKEER DVL+ RM EMGLPREQYEWYL+I RHG VKHSGFSLAFDRF+LF
Sbjct: 541 PKGGVVISGSQKEERLDVLMRRMGEMGLPREQYEWYLDIHRHGAVKHSGFSLAFDRFLLF 600

Query: 601 TTGLTDIKDVIPFPRSFGKASN 621
           TTGLTDIKDVIPFPRSFGKAS+
Sbjct: 601 TTGLTDIKDVIPFPRSFGKASD 622

[Close]

BLAST of Tan0012345 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 604.7 bits (1558), Expect = 8.0e-173
Identity = 332/628 (52.87%), Postives = 440/628 (70.06%), Query Frame = 0


Query: 11  PLTFSKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVREAPSPADATVRA--- 70
           P+T SKYSKRV LKT+LD +D G G    ++V+GGWVKS++ V + +P P    V A   
Sbjct: 20  PITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLPVVAAPSP 79

Query: 71  -AGEALPPRAKDVSCIELIQSRIPIVRSLLKMLYGNNLHGG------EKLETPVQK--PP 130
            +G        ++ C E+IQS++ I +    +L G    GG      +K E   QK  PP
Sbjct: 80  SSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSG----GGKTYPIFDKTELAGQKAVPP 139

Query: 131 LPSTAFLVVNDGSCVASLQVVIESSLH--HPGQLIPTGTCILVEGILKRAPV--PGKHVV 190
                + +++DGS ++SLQVV++S+L      QL+  GTCI+ EG+L R P+    KHV+
Sbjct: 140 PEYVFYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVL-RLPLAASAKHVI 199

Query: 191 RLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTF 250
            LE EK+LHVG V+ EKYPLS+K+LP+  LR FS+FRPRTTTV SV R+ +ALT A+HTF
Sbjct: 200 ELEAEKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTF 259

Query: 251 FQNHGFLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAA 310
            Q HGF YVQ+P ITTT   GF E F VTT+ GK+  K+ +    +E DG S++TVKA  
Sbjct: 260 LQYHGFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEEKKPPVQEKDGFSIDTVKAVI 319

Query: 311 IEKSNLVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKS--KKDKVHFSENF 370
            EK+ L+  L+RS+SNRE +VAAV DLKKTN+LA Q+E + K K+ +  K +K+ FS++F
Sbjct: 320 KEKTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDF 379

Query: 371 FPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELED 430
           F ++TYLT SG+ HLE+YA ALG VY+ GPRF ADKI++A+H AE W VE E+AFAEL+D
Sbjct: 380 FGRDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDD 439

Query: 431 VMNCADDLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILD 490
            M+CAD+  KFL K+VLE+  +DMKF+ KR+DKT   RLE+  +SS  + SYT  I +L 
Sbjct: 440 AMDCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQ 499

Query: 491 KATDQKSQHLKKLKFGAALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVA 550
           KAT  K +   K ++G ALT +HLSYL D IYK PVIV  YPK  K F VRLNDD +TVA
Sbjct: 500 KATTTKFE--TKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLNDDKKTVA 559

Query: 551 AFDVVLPKGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAF 610
           AFD+V+PK GVVI+GSQ EERF++L  R+ E G  RE++EWYL+++RHGTVKHSG SL+ 
Sbjct: 560 AFDLVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSM 619

Query: 611 DRFVLFTTGLTDIKDVIPFPRSFGKASN 621
           ++ +L+ TGL DIKD IPFPRS+GKA+N
Sbjct: 620 EQMLLYATGLPDIKDAIPFPRSWGKANN 638

[Close]

BLAST of Tan0012345 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 471.1 bits (1211), Expect = 1.4e-132
Identity = 251/609 (41.22%), Postives = 370/609 (60.76%), Query Frame = 0


Query: 15  SKYSKRVLLKTVLDSTDGGLGFVDHKLVVGGWVKSSKEVVREAPSPADATVRAAGEALPP 74
           +++S RVL++T+LD  DGG G     + +GGWVKS ++  +                   
Sbjct: 27  AQFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRDQGKR------------------ 86

Query: 75  RAKDVSCIELIQSRIPIVRSLLKMLYGNNLHGGEKLETPVQKPPLPSTAFLVVNDGSCVA 134
                                                         + +FL VNDGSC A
Sbjct: 87  ----------------------------------------------TFSFLAVNDGSCPA 146

Query: 135 SLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPGK---HVVRLEVEKILHVGKVEQEK 194
           +LQV+++ SL+    L+ TGTC+ V+G+LK  P  GK     + L V K++ VG V+  K
Sbjct: 147 NLQVMVDPSLYDVSNLVATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDASK 206

Query: 195 YPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGFLYVQLPTITTT 254
           YPL + KL ++ LR   + R RT ++++V RIRNAL FATH+FFQ H FLY+  P ITT+
Sbjct: 207 YPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITTS 266

Query: 255 DSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKSNLVKELERSESNR 314
           D EG  E F  TT+   +   + +  ++       +E  +   IE+ N+V EL+ +++++
Sbjct: 267 DCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKASK 326

Query: 315 EALVAAVQDLKKTNELALQLEARGKLK-SKSKKD-KVHFSENFFPQETYLTVSGQLHLET 374
           EA+ AAV +LK   E    ++ R +L+    KKD  + +S++FF ++ +LTVSGQL +ET
Sbjct: 327 EAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQVET 386

Query: 375 YACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCADDLVKFLSKWVL 434
           YACAL NVY+ GP FRA+   +++H AE WMVE EIAFA+LED MNCA+  VK++  W+L
Sbjct: 387 YACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWLL 446

Query: 435 EHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATDQKSQHLKKLKFGA 494
           E C  DM+ + K  D   I RL+   ++ F +++YT AIE+L++A  +  +    +++G 
Sbjct: 447 EKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGKEFDNNVEWGI 506

Query: 495 ALTADHLSYLADAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFDVVLPKGGVVISGSQ 554
            L ++H  YL + +++KP+IV+NYPK  K F +RLNDD +TVAA DV++PK G +I GSQ
Sbjct: 507 DLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQ 566

Query: 555 KEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVI 614
           +EER+DV+  R++EMGLP E YEWYL+++R+GTVKH GF L F+R +LF TGL +I+DVI
Sbjct: 567 REERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHCGFGLGFERMILFATGLDNIRDVI 570

Query: 615 PFPRSFGKA 619
           PFPR  GKA
Sbjct: 627 PFPRYPGKA 570

[Close]

BLAST of Tan0012345 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 439.5 bits (1129), Expect = 4.5e-123
Identity = 230/502 (45.82%), Postives = 333/502 (66.33%), Query Frame = 0


Query: 123 AFLVVNDGSCVASLQVVIESSLHHPGQLIPTGTCILVEGILKRAPVPG--KHVVRLEVEK 182
           AFL VNDGSC A+LQV+++SSL+   +L+ TGTC+ V+G+LK  P     K  + L VE 
Sbjct: 68  AFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGKGLKQSIELSVET 127

Query: 183 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHGF 242
           ++ VG V+   YPL + KL  +FLR   + R RT  +++V RIRNAL FATH+FFQ H F
Sbjct: 128 VIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSF 187

Query: 243 LYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIEKSNL 302
           LY+  P ITT+D EG  E F VTT+   +   + +  ++       +E  +    E+   
Sbjct: 188 LYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGEA 247

Query: 303 VKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKD--KVHFSENFFPQETY 362
           V +L+ +++++E + A+V  L         +E R +LK    K+  K+ +S +FF ++ +
Sbjct: 248 VAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAF 307

Query: 363 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCAD 422
           LTVSGQL +ETYACAL +VY+ GP FRA+   +++H AE WMVE EIAFA++ D MNCA+
Sbjct: 308 LTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAE 367

Query: 423 DLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATDQ- 482
             VK++ KW+++ C DDM+ + K +D+    RL     +SF++V+YT AIE L+KA  Q 
Sbjct: 368 AYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQG 427

Query: 483 KSQHLKKLKFGAALTADHLSYLADAIY-KKPVIVFNYPKETKPFNVRLNDDGQTVAAFDV 542
           K     K+++G  L ++H  YL +  + +KP+IV+NYPK  K F +RLNDD +TVAA DV
Sbjct: 428 KVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDV 487

Query: 543 VLPKGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFV 602
           ++PK G +I GSQ+EER+DV+  R++EMGLP E YEWYL+++R+GTVKH GF L F+R +
Sbjct: 488 LVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKHCGFGLGFERMI 547

Query: 603 LFTTGLTDIKDVIPFPRSFGKA 619
            F TG+ +I+DVIPFPR  GKA
Sbjct: 548 QFATGIDNIRDVIPFPRYPGKA 569

[Close]

BLAST of Tan0012345 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 330.5 bits (846), Expect = 2.9e-90
Identity = 194/499 (38.88%), Postives = 291/499 (58.32%), Query Frame = 0


Query: 121 STAFLVVNDGSCVASLQVVIESSLHHPGQL----IPTGTCILVEGILKRAPVPGKHVVRL 180
           S  F+ +NDGSC+++LQ V+ S      Q+    I TG  + V+G +  A    K  V L
Sbjct: 126 SVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIV-ASQGTKQKVEL 185

Query: 181 EVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQ 240
           +VEKI+ VG+ +   YP+ +K++  +FLR  ++ RPRT T  +V R+RN L +ATH FFQ
Sbjct: 186 KVEKIIVVGECD-SSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQ 245

Query: 241 NHGFLYVQLPTITTTDSEGFSEKFHVTTVAGKSAGKKVEPTEDKETDGVSLETVKAAAIE 300
             GF++V  P IT +D EG  E+F VTT+          P+  + TD  S++ +      
Sbjct: 246 ESGFVWVASPIITASDCEGAGEQFCVTTLI---------PSSHENTD-TSIDAI------ 305

Query: 301 KSNLVKELERSESNREALVAAVQDLKKTNELALQLEARGKLKSKSKKDKVHFSENFFPQE 360
                                                      K+K   + +S++FF + 
Sbjct: 306 ------------------------------------------PKTKGGLIDWSQDFFGKP 365

Query: 361 TYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNC 420
            +LTVSGQL+ ETYA AL +VY+ GP FRA+   +++H AE WM+E E+AFA+L+D M C
Sbjct: 366 AFLTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMAC 425

Query: 421 ADDLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESFITSSFEKVSYTAAIEILDKATD 480
           A   ++++ K+VL++C +DM+F    I+K  I RL       F ++ YT AIEIL KA  
Sbjct: 426 ATAYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEILLKANK 485

Query: 481 QKSQHLKKLKFGAALTADHLSYLA-DAIYKKPVIVFNYPKETKPFNVRLNDDGQTVAAFD 540
           +       +K+G  L ++H  Y+  +A   +PVI+ +YPKE K F +R NDDG+TVAA D
Sbjct: 486 KFD---FPVKWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMD 545

Query: 541 VVLPKGGVVISGSQKEERFDVLITRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRF 600
           +++P+ G +I GSQ+EER +VL  R+ E+ L +E Y WYL+++R+G+V H+GF L F+R 
Sbjct: 546 MLVPRIGELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERL 561

Query: 601 VLFTTGLTDIKDVIPFPRS 615
           V F TG+ +I+DVIPFPR+
Sbjct: 606 VQFVTGIDNIRDVIPFPRT 561

[Close]

BLAST of Tan0012345 vs. TAIR 10
Match: AT1G68420.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 1.8e-15
Identity = 35/77 (45.45%), Postives = 57/77 (74.03%), Query Frame = 0


Query: 374 LGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELEDVMNCADDLVKFLSKWVLEHCL 433
           +G+VY+  P FRA+K  +++H AE WMVEVE+AFA +E+ MNC++ +VK +   +LE C 
Sbjct: 1   MGDVYTFAPTFRAEKSHTSRHLAEFWMVEVELAFAGVEEAMNCSEAVVKDMCTTLLEKCR 60

Query: 434 DDMKFVQKRIDKTSIHR 451
           DDM+++ +++D+  I R
Sbjct: 61  DDMEYMVEKVDEFCIDR 77

The following BLAST results are available for this feature:

BLAST of Tan0012345 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SW95	1.1e-171	52.87	Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 ...	[more]
Q9SW96	2.0e-131	41.22	Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 ...	[more]
Q9SSK1	6.3e-122	45.82	Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 ...	[more]
Q6MEC9	1.2e-96	38.96	Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G...	[more]
P52276	2.9e-95	39.56	Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117...	[more]

BLAST of Tan0012345 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023004185.1	0.0e+00	90.35	asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita maxima]	[more]
XP_023514428.1	0.0e+00	90.19	asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_038899280.1	0.0e+00	89.86	asparagine--tRNA ligase, cytoplasmic 2 [Benincasa hispida]	[more]
XP_022959835.1	0.0e+00	89.07	asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita moschata]	[more]
KAG6593030.1	0.0e+00	87.66	Asparagine--tRNA ligase, cytoplasmic 2, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of Tan0012345 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KTV9	0.0e+00	90.35	asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1H7G6	0.0e+00	89.07	asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A5D3BD07	1.3e-308	87.92	Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3C6Z5	4.3e-307	87.92	Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1	[more]
A0A6J1GR04	6.9e-305	86.01	Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456649 PE=3 SV=1	[more]

BLAST of Tan0012345 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07420.1	8.0e-173	52.87	asparaginyl-tRNA synthetase 2	[more]
AT5G56680.1	1.4e-132	41.22	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT1G70980.1	4.5e-123	45.82	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT4G17300.1	2.9e-90	38.88	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT1G68420.1	1.8e-15	45.45	Class II aaRS and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 290..324
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 193..619 e-value: 1.8E-86 score: 292.6
None	No IPR available	PANTHER	PTHR22594	ASPARTYL/LYSYL-TRNA SYNTHETASE	coord: 16..620
None	No IPR available	PANTHER	PTHR22594:SF36	ASPARAGINE--TRNA LIGASE, CYTOPLASMIC 2-RELATED	coord: 16..620
None	No IPR available	CDD	cd04318	EcAsnRS_like_N	coord: 123..186 e-value: 8.09617E-15 score: 67.9757
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 201..614
IPR002312	Aspartyl/Asparaginyl-tRNA synthetase, class IIb	PRINTS	PR01042	TRNASYNTHASP	coord: 359..371 score: 41.78 coord: 535..551 score: 27.94 coord: 575..589 score: 33.94 coord: 375..388 score: 32.47
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 202..264 e-value: 4.2E-9 score: 35.9
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 344..613 e-value: 1.5E-51 score: 175.3
IPR004522	Asparagine-tRNA ligase	TIGRFAM	TIGR00457	TIGR00457	coord: 122..617 e-value: 2.5E-127 score: 423.4
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 377..610 score: 8.775076

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0012345.1	Tan0012345.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006418	tRNA aminoacylation for protein translation
biological_process	GO:0006421	asparaginyl-tRNA aminoacylation
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004816	asparagine-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding