Tan0012317 (gene) Snake gourd v1

Overview


Name	Tan0012317
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	RING-type E3 ubiquitin transferase
Location	LG01: 105228112 .. 105230981 (-)
RNA-Seq Expression	Tan0012317
Synteny	Tan0012317

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCTACGGATGTCAATTTCAACCTTCTTTCACTTGATGCATGTTGATGACAACCTTCCTCAAAATCTAGGTATCAAAATTTCAAATTTTATTAAAAATGAGTTGAAGATAACCTCTTGCTCTAGTTGAATTTTATGTACAGTAAAGAAGGTTGAAGATGGACTCTTGCTTTCATGGAATTTTATCGTACTCTCGCTCGTTGAAGATAGCCCCTCGCTCGCTTTGAGTTTTAGTGTACTCTCGATCTCAACGTGAAGTCTAAGATAAGGTATGGTTGTTTTAAAAGAACAATTCTCCATTGTTTTCACCAAAAAAAAAAAAAAACTATCTTCTTCTTCCCGGAGAAGGCAGCCGTTAATGCATCTTCCCATTCCAAAACTCAAACACCACAAAATTAAGGCTACCAACAAGACAATTGTTTACACCATCTCCCACTCATCCTTTTCTTCATCTCCACGAAACACTCTGAAACTAAACAAACCTCAAGATAGAGAAATGGGCGATGGCAATGTGAATTGCTTTATAAAATCTTTAAAACAAACCCAGAAAGAAAGCCATTCCTTCCTCCTCCCCTCCCTCCATCATTCTCCCATAATTTATAAAATCTTTCCTTCAGATTCCAATTCCCAAATCAGAAAAAAAATAATCACCAATTTCCAACATGTCTTTGAGCCCTCCTCGACAAAGAACCAACAACCGCCATACCACAACCTTCCACCTCTACTGGTGCTACCAATGCCACCGGATGGTCACCCTCGCCGCCGCAGACCCCTCCGAGCTCTTCTGCCCGCGCTGTTCCGGCCAGTTCCTCGAAGAACTCCATCTCACCCGCCCCGAACTCCATCCCTCGCCGGAGACTCGTTTACTTGAAGCCCTCTCCCTAATGCTCGACCCGCCGGTTCGCCTCTTTAGACTCCCCGACTCGAACCGCCGCCGTCCACCGCTGCACCAGTTCGACGATGTCGACCGACGGCCGTTCAGTGATCTCGAAGGCGACGAGTGGTTCCACGGGCGAACACGGCGGCGCACCCGAAGCATGGACGAGAGGGCCGATTCCGGCCTGCAACCCCCTAATCCGAACCGGTCTCGGACTGTCATCATTCTCAGACCCACAGACCCATCTCGGCCGCTTCAGCCCATAATACCAACGAGAGCAGACCCAAGAGACTACTTCACCGGACCACAACTCGACGAACTAATCGAAGAACTGACGCAGAACGACCGGCCGGGGCCGCCACCGGCGAACGAGGCGGCGATAGAGGGCATACCGACGGTGAAGATAGAGGCTGAGCATCTGAAGAACGACTCGATGTGTCCAGTTTGTAAGGAGGAGTTTGAGGTTGGGGGAGAAGCAAGGGAATTGAGCTGCAAGCATATATACCATTCGGAGTGCATAGTGCCATGGCTTCGGCTTCACAATTCTTGTCCGGTTTGCAGGCAAGAGATGCGGCCATCGCCGCCGGAGAATCAAGCGGCGGAAACTGAAAGTTCTGAAGATGGAGAAGGGATTGGGAGAAGATGTGCCAGGTGGTGGGGTCAGTTGGTGTCTAGGCCTTTTCGGAATAGGTATAGGCAGCTTAGTCCTCCACAAGACCGCTACAATTCTTCTCGAGGTGATGTCTAATTTATTTCTTATATATATATGTTCGATATATTGGAAAGTTTCTAGATAATATTGATATAAATATAAGGTATTCAATTAAATAAAGTTTGTATGAGCATAAACTAGAGATGCACATACAATAATTAGGGATCTCTCGATGGTGGTGTATTTTTAACATTCATTATCTGAATAATGGGCTACAAAATCATGTTTAAAACTTTTCTTATTTTCATTTACGTGAAATTCATAAGGCTTATATATTGGATTGTCATTCTTCTCATCATTCCAACCCTTCCGGGAAGAAATCGATAGAAGTATTACCTTCTCGAAAAATAAAAATGAATACAATCATTTTATGACAAAAAAATACGTTAGATAAAATTTAACAAAATACTACATAAAAAGCAAATATTTAACAAAATACTACATATCAAAGCAAATATCAACACTACAAAGGGTTAAAAAAAAACTCTACTTTCTTAAGGACCATAGGCTCAAATAACCTCATTTTAAAGTTGTTTGAAAAATGAGTTATAATAACATTGATTATAATAGTATGTAAAATTATATAATATTATTTAAAATGCAGAATTACTTAATATAAGGATATAATATTGCAAATATAAGAATAAAAATTTGATGAATTTTTCTACATTGAACATAAAAGCATAAAAGTTGATTTTTTTTAAAAAAAAAAACGCTTCTGGTAAAAAAACATATTCTTTCAATCATAGATTCTGACTTAGACATTTTATTCCATTTTCCAGAAAGTAATTCAAGGGGTGGTTTATTCCACCTCTTTCCCTTTGCCCTTCTACTGTCGATGTTAATGCTGCAAATAACTCGGATTTTACTTGAACGTATTCGTTAGGAATGTGAATTCGTATGCAACGGATTTTACTTGAACGTATTCGTTAGGAATGTGAATTCGTATGCAAATGTGAAGACACTGTGGAAAAGACAGCATATAATTAGATTCCTTACCACGGTGCATTTGGTATGATGCCACTCCACTGCCTTCTTTCAAATGTCTTCTAATTATATTCTACAGTTTCAGCAAAAGCACTAATCAGATGATTCAACTTTTTGATCTGCAGGATTGAGATAGTCCACAAAGATCGCAGTCTCCAACATACATCATTTCTTATACCTGATTGTGTTAGCTCAAAGCATGTTCCATGTTCTAAACTAAAACATTTTCCTGAAGCACATGCCCATATATTAAACTGGCAGGATGTAATTTCTACCCACTCATGCAATAAATGCA

mRNA sequence

CTTCTACGGATGTCAATTTCAACCTTCTTTCACTTGATGCATGTTGATGACAACCTTCCTCAAAATCTAGGTATCAAAATTTCAAATTTTATTAAAAATGAGTTGAAGATAACCTCTTGCTCTAGTTGAATTTTATGTACAGTAAAGAAGGTTGAAGATGGACTCTTGCTTTCATGGAATTTTATCGTACTCTCGCTCGTTGAAGATAGCCCCTCGCTCGCTTTGAGTTTTAGTGTACTCTCGATCTCAACGTGAAGTCTAAGATAAGGTATGGTTGTTTTAAAAGAACAATTCTCCATTGTTTTCACCAAAAAAAAAAAAAAACTATCTTCTTCTTCCCGGAGAAGGCAGCCGTTAATGCATCTTCCCATTCCAAAACTCAAACACCACAAAATTAAGGCTACCAACAAGACAATTGTTTACACCATCTCCCACTCATCCTTTTCTTCATCTCCACGAAACACTCTGAAACTAAACAAACCTCAAGATAGAGAAATGGGCGATGGCAATGTGAATTGCTTTATAAAATCTTTAAAACAAACCCAGAAAGAAAGCCATTCCTTCCTCCTCCCCTCCCTCCATCATTCTCCCATAATTTATAAAATCTTTCCTTCAGATTCCAATTCCCAAATCAGAAAAAAAATAATCACCAATTTCCAACATGTCTTTGAGCCCTCCTCGACAAAGAACCAACAACCGCCATACCACAACCTTCCACCTCTACTGGTGCTACCAATGCCACCGGATGGTCACCCTCGCCGCCGCAGACCCCTCCGAGCTCTTCTGCCCGCGCTGTTCCGGCCAGTTCCTCGAAGAACTCCATCTCACCCGCCCCGAACTCCATCCCTCGCCGGAGACTCGTTTACTTGAAGCCCTCTCCCTAATGCTCGACCCGCCGGTTCGCCTCTTTAGACTCCCCGACTCGAACCGCCGCCGTCCACCGCTGCACCAGTTCGACGATGTCGACCGACGGCCGTTCAGTGATCTCGAAGGCGACGAGTGGTTCCACGGGCGAACACGGCGGCGCACCCGAAGCATGGACGAGAGGGCCGATTCCGGCCTGCAACCCCCTAATCCGAACCGGTCTCGGACTGTCATCATTCTCAGACCCACAGACCCATCTCGGCCGCTTCAGCCCATAATACCAACGAGAGCAGACCCAAGAGACTACTTCACCGGACCACAACTCGACGAACTAATCGAAGAACTGACGCAGAACGACCGGCCGGGGCCGCCACCGGCGAACGAGGCGGCGATAGAGGGCATACCGACGGTGAAGATAGAGGCTGAGCATCTGAAGAACGACTCGATGTGTCCAGTTTGTAAGGAGGAGTTTGAGGTTGGGGGAGAAGCAAGGGAATTGAGCTGCAAGCATATATACCATTCGGAGTGCATAGTGCCATGGCTTCGGCTTCACAATTCTTGTCCGGTTTGCAGGCAAGAGATGCGGCCATCGCCGCCGGAGAATCAAGCGGCGGAAACTGAAAGTTCTGAAGATGGAGAAGGGATTGGGAGAAGATGTGCCAGGTGGTGGGGTCAGTTGGTGTCTAGGCCTTTTCGGAATAGGTATAGGCAGCTTAGTCCTCCACAAGACCGCTACAATTCTTCTCGAGGAATGTGAATTCGTATGCAACGGATTTTACTTGAACGTATTCGTTAGGAATGTGAATTCGTATGCAAATGTGAAGACACTGTGGAAAAGACAGCATATAATTAGATTCCTTACCACGGTGCATTTGGATTGAGATAGTCCACAAAGATCGCAGTCTCCAACATACATCATTTCTTATACCTGATTGTGTTAGCTCAAAGCATGTTCCATGTTCTAAACTAAAACATTTTCCTGAAGCACATGCCCATATATTAAACTGGCAGGATGTAATTTCTACCCACTCATGCAATAAATGCA

Coding sequence (CDS)

ATGTCTTTGAGCCCTCCTCGACAAAGAACCAACAACCGCCATACCACAACCTTCCACCTCTACTGGTGCTACCAATGCCACCGGATGGTCACCCTCGCCGCCGCAGACCCCTCCGAGCTCTTCTGCCCGCGCTGTTCCGGCCAGTTCCTCGAAGAACTCCATCTCACCCGCCCCGAACTCCATCCCTCGCCGGAGACTCGTTTACTTGAAGCCCTCTCCCTAATGCTCGACCCGCCGGTTCGCCTCTTTAGACTCCCCGACTCGAACCGCCGCCGTCCACCGCTGCACCAGTTCGACGATGTCGACCGACGGCCGTTCAGTGATCTCGAAGGCGACGAGTGGTTCCACGGGCGAACACGGCGGCGCACCCGAAGCATGGACGAGAGGGCCGATTCCGGCCTGCAACCCCCTAATCCGAACCGGTCTCGGACTGTCATCATTCTCAGACCCACAGACCCATCTCGGCCGCTTCAGCCCATAATACCAACGAGAGCAGACCCAAGAGACTACTTCACCGGACCACAACTCGACGAACTAATCGAAGAACTGACGCAGAACGACCGGCCGGGGCCGCCACCGGCGAACGAGGCGGCGATAGAGGGCATACCGACGGTGAAGATAGAGGCTGAGCATCTGAAGAACGACTCGATGTGTCCAGTTTGTAAGGAGGAGTTTGAGGTTGGGGGAGAAGCAAGGGAATTGAGCTGCAAGCATATATACCATTCGGAGTGCATAGTGCCATGGCTTCGGCTTCACAATTCTTGTCCGGTTTGCAGGCAAGAGATGCGGCCATCGCCGCCGGAGAATCAAGCGGCGGAAACTGAAAGTTCTGAAGATGGAGAAGGGATTGGGAGAAGATGTGCCAGGTGGTGGGGTCAGTTGGTGTCTAGGCCTTTTCGGAATAGGTATAGGCAGCTTAGTCCTCCACAAGACCGCTACAATTCTTCTCGAGGAATGTGA

Protein sequence

MSLSPPRQRTNNRHTTTFHLYWCYQCHRMVTLAAADPSELFCPRCSGQFLEELHLTRPELHPSPETRLLEALSLMLDPPVRLFRLPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGRTRRRTRSMDERADSGLQPPNPNRSRTVIILRPTDPSRPLQPIIPTRADPRDYFTGPQLDELIEELTQNDRPGPPPANEAAIEGIPTVKIEAEHLKNDSMCPVCKEEFEVGGEARELSCKHIYHSECIVPWLRLHNSCPVCRQEMRPSPPENQAAETESSEDGEGIGRRCARWWGQLVSRPFRNRYRQLSPPQDRYNSSRGM

Homology

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BLAST of Tan0012317 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 7.4e-34
Identity = 98/275 (35.64%), Postives = 142/275 (51.64%), Query Frame = 0


Query: 21  YWCYQCHRMVTLAAADPSELFCPRCSGQFLEELHLTRPELH----PSPETRLLEALSLML 80
           +WC++C R V L A D     C  C G F+EE+ +     H      P   L+EA S  +
Sbjct: 8   HWCHRCQRAVWLRARD---AVCSYCGGGFVEEIDIGPSRAHRDVERDPTFDLMEAFSAFM 67

Query: 81  DPPVRLFRLPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGRTRRRTRSMDERADSGLQP 140
                  RL + +  R    +        FS+L     F G+   R    D   +S ++ 
Sbjct: 68  RS-----RLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGD---NSSVEA 127

Query: 141 PNPNRSRTVIILRPTDPSRPLQPIIPTRADPRDYFTGPQLDELIEELTQ-NDRPGPPPAN 200
                +  + I R T+                DYF GP L+ELIE+L+      GPPPA 
Sbjct: 128 FVNGAAPGIGIARGTNAG--------------DYFFGPGLEELIEQLSSGTHHRGPPPAP 187

Query: 201 EAAIEGIPTVKIEAEHLK-NDSMCPVCKEEFEVGGEARELSCKHIYHSECIVPWLRLHNS 260
           +++I+ +PT+KI  +HLK +DS CPVCK+EFE+  EA+++ C HIYHS+CIVPWL  HNS
Sbjct: 188 KSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNS 247

Query: 261 CPVCRQEMRPSPPENQAAETESSEDGEGIGRRCAR 290
           CPVCR+E+   P    ++ T+SS++    GR  +R
Sbjct: 248 CPVCRKEL---PSRGSSSSTQSSQNRSTNGRENSR 254

[Close]

BLAST of Tan0012317 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-32
Identity = 94/297 (31.65%), Postives = 153/297 (51.52%), Query Frame = 0


Query: 20  LYWCYQCHRMVTLAAADPSELFCPRCSGQFLEELHLTRPELHPSPETRLLEALSLMLDPP 79
           +++CYQC++ VT++ +  ++ FCP C+  FLEE     P    +      ++   M DP 
Sbjct: 20  MFFCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSSDSFFPMADPF 79

Query: 80  VRLFRLPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGRTRRRTRSMDERADSGLQPPNP 139
             L  L   +    P            S ++    F    + + RS  +   S    P  
Sbjct: 80  STLLPLIFGSSAAAP------------SGMDFMSLFGPSMQPQARSTQQNPQSDAFDPFT 139

Query: 140 NRSRTVIILRPTD-------PSRPLQPIIPTRADPRDYFTGPQLDELIEELTQND--RPG 199
                +  LR +         + P  P      +  DYF GP L++LI++L +ND  R G
Sbjct: 140 FLQNHLQTLRSSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYG 199

Query: 200 PPPANEAAIEGIPTVKIEAEHLKND-SMCPVCKEEFEVGGEARELSCKHIYHSECIVPWL 259
            PPA+++AI+ +PTVK+  + LK++ + C VC +EFE G + +++ CKH++H +C++PWL
Sbjct: 200 TPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWL 259

Query: 260 RLHNSCPVCRQEMRPSPP--ENQAAETESSEDGEGI--GRRCARWWGQLVSRPFRNR 303
            LHNSCPVCR E+    P  EN++  ++ S DG+G   G++  R+  QL   PFR +
Sbjct: 260 ELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQGSVEGQQTPRFSIQL-PWPFRRQ 303

[Close]

BLAST of Tan0012317 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.5e-31
Identity = 99/294 (33.67%), Postives = 137/294 (46.60%), Query Frame = 0


Query: 21  YWCYQCHRMVTLAAADPSELFCPRCSGQFLEELHLTRPELHPSPETRLLEALSLMLDPPV 80
           +WC++C R V L   +P    C  C G F+EEL + +                     P 
Sbjct: 8   HWCHRCQRAVRLHGQEP---VCFYCGGGFVEELDMAQAS-------------------PF 67

Query: 81  RLFR----LPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGRTRRRTRSMDERADSGLQP 140
            +FR    + + ++    +  F    R   ++   D    G    RT S       GL P
Sbjct: 68  DMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRG----RTISSGPENFPGLAP 127

Query: 141 PNPNRSRTVIILRPTDPSRPL-----QPIIPTRADPRDYFTGPQLDELIEELTQ-NDRPG 200
                 +    L   +    L       I  TR +  DYF GP L+EL E+L+    R G
Sbjct: 128 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 187

Query: 201 PPPANEAAIEGIPTVKIEAEHLK-NDSMCPVCKEEFEVGGEARELSCKHIYHSECIVPWL 260
           PPPA  +AI+ +PT+KI   HL+ +DS CPVCK+EFE+G EA+++ C HIYHS+CIVPWL
Sbjct: 188 PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWL 247

Query: 261 RLHNSCPVCRQEM----RPSPPENQAAETESSEDGEGIGRRCARWWGQLVSRPF 300
             HNSCPVCRQE+     PS  +N+   T +           +R  G     PF
Sbjct: 248 VQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPF 275

[Close]

BLAST of Tan0012317 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.5e-31
Identity = 98/287 (34.15%), Postives = 143/287 (49.83%), Query Frame = 0


Query: 21  YWCYQCHRMVTLAAADP-SELFCPRCSGQFLEELHLTRPE-----LHPSPETRLLEALSL 80
           ++CYQC+R V +  + P S+  CP C+  FLEE     P      L+P+P +     L L
Sbjct: 23  FFCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLFL 82

Query: 81  ML-DPPVRLFRL--PDSNRRRPPLHQFDDVDR----RPFSDLEGDEWFHGRTRRRTRSMD 140
            L DP   L  L  P S+          D +      P     GD +           ++
Sbjct: 83  TLSDPFASLLPLLFPSSSSTTTSSSASIDPNNPSLSGPTRSGRGDPFAFDPFTFIQNHLN 142

Query: 141 ERADSGLQPPNPNRSRTVIILRPTDPSRPLQPIIPTRADPRDYFTGPQLDELIEELTQND 200
           +   SG Q         VI   P+D    L       A+  DYF GP L++LI++L +ND
Sbjct: 143 DLRSSGAQ------IEFVIQNNPSDQGFRLP------ANIGDYFIGPGLEQLIQQLAEND 202

Query: 201 --RPGPPPANEAAIEGIPTVKIEAEHLKND-SMCPVCKEEFEVGGEARELSCKHIYHSEC 260
             R G PPA+++AIE +P V I   +L ++ + C VC ++FE G EA+++ CKH+YH +C
Sbjct: 203 PNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDC 262

Query: 261 IVPWLRLHNSCPVCRQEMRPSPPENQ-----AAETESSEDGEGIGRR 287
           ++PWL LHNSCPVCR E+    P+ +     A  T    DG+  G+R
Sbjct: 263 LLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTSGGNDGDNSGQR 297

[Close]

BLAST of Tan0012317 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.3e-25
Identity = 92/296 (31.08%), Postives = 137/296 (46.28%), Query Frame = 0


Query: 2   SLSPPRQRTNNRHTTTFHL---YWCYQCHRMVTLAAADPSE--LFCPRCSGQFLEELHLT 61
           +++P  + T    TTT  L   YWCY C R +++     +   + CP C+G F+EE+  +
Sbjct: 9   TITPTTESTTTTTTTTTTLTTSYWCYSCTRFISVWEDQDANAGVLCPYCNGGFIEEIEDS 68

Query: 62  RPEL-----HPSPETRLLEALSLMLDPPVRLFRLPDSNRRRP--PLHQFDDVDRRPFSDL 121
                      +PE R +E      +    + R   SNRR    P+            + 
Sbjct: 69  SNSTVAAIPASTPEVRSVE------ETHRSIIRRRRSNRRTSFNPVIVLHGGGGGGAGER 128

Query: 122 EGDEWFHGRTRRRTR---SMDERADSGLQPPNPNRSRTVIILRPTDPSRPLQPIIPTRAD 181
             +E   G TR R       D+ + SGL+P   + S  ++                    
Sbjct: 129 VENEEGDGATRERRAYEFYYDDGSGSGLRPLPDSVSEILM-------------------- 188

Query: 182 PRDYFTGPQLDELIEELTQND-------RPGPPPANEAAIEGIPTVKIEAEHLKNDSMCP 241
                 G   + L+E+L+Q +       R G PPA+++AIE +P V+I   H K ++ C 
Sbjct: 189 ------GSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDCHTKAEANCA 248

Query: 242 VCKEEFEVGGEARELSCKHIYHSECIVPWLRLHNSCPVCRQEMRPSPPENQAAETE 276
           VC E FE G E RE+ CKHI+H +CIVPWL + NSCPVCR E+ PS P  ++ E E
Sbjct: 249 VCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFEL-PSDPIQRSNEEE 271

[Close]

BLAST of Tan0012317 vs. NCBI nr
Match: XP_022941465.1 (E3 ubiquitin-protein ligase RZF1-like [Cucurbita moschata])

HSP 1 Score: 526.9 bits (1356), Expect = 1.2e-145
Identity = 255/308 (82.79%), Postives = 276/308 (89.61%), Query Frame = 0


Query: 11  NNRHT-TTFHLYWCYQCHRMVTLAAA-DPSELFCPRCSGQFLEELHLTRPELHPSPETRL 70
           ++RHT ++FH YWC+QCHRMVTLA   DPSEL C RCSGQFLEELHLTRP+   SPETRL
Sbjct: 5   SSRHTRSSFHFYWCHQCHRMVTLATTRDPSELICSRCSGQFLEELHLTRPQFDNSPETRL 64

Query: 71  LEALSLMLDPPVRLFRLPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGRTRRRTRSMDE 130
           LEALSLMLDPPVRLFRLPD +RRRP LHQFD+ DR+ FSD EGDEWFH RTRRR RS+DE
Sbjct: 65  LEALSLMLDPPVRLFRLPDIDRRRPMLHQFDNFDRQSFSDPEGDEWFHWRTRRRNRSLDE 124

Query: 131 RADSGLQPPNPNRSRTVIILRPTDPSRPLQPIIPTRADPRDYFTGPQLDELIEELTQNDR 190
           R +S LQPPN NRSRTVIILRPTDPSRP+QPIIP RADPRDYFTGP+LDELIEELTQNDR
Sbjct: 125 RDNSSLQPPNQNRSRTVIILRPTDPSRPVQPIIPPRADPRDYFTGPRLDELIEELTQNDR 184

Query: 191 PGPPPANEAAIEGIPTVKIEAEHLKNDSMCPVCKEEFEVGGEARELSCKHIYHSECIVPW 250
           PGP PA+EAAIE IPTVKI AEHLKND+MCPVCKEEFE+G EAREL CKH+YHSECIVPW
Sbjct: 185 PGPQPASEAAIERIPTVKIAAEHLKNDTMCPVCKEEFEIGEEARELMCKHVYHSECIVPW 244

Query: 251 LRLHNSCPVCRQEMRPSPPENQAAETESSEDGEGIGRRCARWWGQLVSRPFRNRYRQLSP 310
           LRLHNSCPVCRQEM PSPPENQAA  ESS+D EGIGRRC RWWGQLV+RPFR+RYRQLSP
Sbjct: 245 LRLHNSCPVCRQEMPPSPPENQAA--ESSDDEEGIGRRCTRWWGQLVNRPFRSRYRQLSP 304

Query: 311 PQDRYNSS 317
           PQD +NSS
Sbjct: 305 PQDSHNSS 310

[Close]

BLAST of Tan0012317 vs. NCBI nr
Match: XP_022981032.1 (E3 ubiquitin-protein ligase RZF1-like [Cucurbita maxima])

HSP 1 Score: 525.8 bits (1353), Expect = 2.6e-145
Identity = 257/308 (83.44%), Postives = 274/308 (88.96%), Query Frame = 0


Query: 11  NNRHT-TTFHLYWCYQCHRMVTLA-AADPSELFCPRCSGQFLEELHLTRPELHPSPETRL 70
           ++RHT  +FHLYWC+QCHRMVTLA A DPSEL C RCSGQFLEELHLTRP+   SPETRL
Sbjct: 5   SSRHTRPSFHLYWCHQCHRMVTLATAGDPSELICSRCSGQFLEELHLTRPQFDNSPETRL 64

Query: 71  LEALSLMLDPPVRLFRLPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGRTRRRTRSMDE 130
           LEALSLMLDPPVRLFRLPD +RRRP  HQFD+ DRR FSD EGDEWFHGRTRRR RS+DE
Sbjct: 65  LEALSLMLDPPVRLFRLPDLDRRRPMSHQFDNFDRRSFSDPEGDEWFHGRTRRRNRSLDE 124

Query: 131 RADSGLQPPNPNRSRTVIILRPTDPSRPLQPIIPTRADPRDYFTGPQLDELIEELTQNDR 190
           R +S L PPN NRSRT IILRPTDPSRP+Q IIP RADP DYFTGP+LDELIEELTQNDR
Sbjct: 125 RDNSSLLPPNQNRSRTFIILRPTDPSRPVQTIIPPRADPGDYFTGPRLDELIEELTQNDR 184

Query: 191 PGPPPANEAAIEGIPTVKIEAEHLKNDSMCPVCKEEFEVGGEARELSCKHIYHSECIVPW 250
           PGP PA+EAAIE IPTVKI AEHLKNDSMCPVCKEEFE+G EAREL CKH+YHSECIVPW
Sbjct: 185 PGPQPASEAAIERIPTVKIAAEHLKNDSMCPVCKEEFEIGEEARELMCKHVYHSECIVPW 244

Query: 251 LRLHNSCPVCRQEMRPSPPENQAAETESSEDGEGIGRRCARWWGQLVSRPFRNRYRQLSP 310
           LRLHNSCPVCRQEM PSPPENQAA  ESSED EGIGRRC RWWGQLV+RPFR+RYRQLSP
Sbjct: 245 LRLHNSCPVCRQEMPPSPPENQAA--ESSEDEEGIGRRCTRWWGQLVNRPFRSRYRQLSP 304

Query: 311 PQDRYNSS 317
           PQDR+NSS
Sbjct: 305 PQDRHNSS 310

[Close]

BLAST of Tan0012317 vs. NCBI nr
Match: XP_023525796.1 (E3 ubiquitin-protein ligase RZF1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 523.9 bits (1348), Expect = 1.0e-144
Identity = 250/308 (81.17%), Postives = 272/308 (88.31%), Query Frame = 0


Query: 11  NNRHT-TTFHLYWCYQCHRMVTLA-AADPSELFCPRCSGQFLEELHLTRPELHPSPETRL 70
           ++RHT ++FH YWC+QCHRMVTLA A DPSEL CPRCS QFLEELHLTRP+   SPETRL
Sbjct: 5   SSRHTRSSFHFYWCHQCHRMVTLATAGDPSELICPRCSAQFLEELHLTRPQFDNSPETRL 64

Query: 71  LEALSLMLDPPVRLFRLPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGRTRRRTRSMDE 130
           L+ALSLMLDPPVRLFRLPD +RRRP  HQFD+ DRR FSD EGDEWFH RTRRR RS+DE
Sbjct: 65  LQALSLMLDPPVRLFRLPDLDRRRPTSHQFDNFDRRSFSDPEGDEWFHWRTRRRNRSLDE 124

Query: 131 RADSGLQPPNPNRSRTVIILRPTDPSRPLQPIIPTRADPRDYFTGPQLDELIEELTQNDR 190
           R +S LQPPN NRSRTVIILRPTD SRP+QPIIP R DPRDYFTGP+LDELIEELTQNDR
Sbjct: 125 RDNSSLQPPNQNRSRTVIILRPTDQSRPVQPIIPPRTDPRDYFTGPRLDELIEELTQNDR 184

Query: 191 PGPPPANEAAIEGIPTVKIEAEHLKNDSMCPVCKEEFEVGGEARELSCKHIYHSECIVPW 250
           PGP PA+EAAIE IPTVKI AEHLKNDS CPVCKEEFE+G EAREL CKH+YHSECIVPW
Sbjct: 185 PGPQPASEAAIERIPTVKIAAEHLKNDSACPVCKEEFEIGEEARELMCKHVYHSECIVPW 244

Query: 251 LRLHNSCPVCRQEMRPSPPENQAAETESSEDGEGIGRRCARWWGQLVSRPFRNRYRQLSP 310
           LRLHNSCPVCRQEM PSPPENQ AE+    +GEGIGRRC RWWGQLV+RPFR+RYRQ+SP
Sbjct: 245 LRLHNSCPVCRQEMPPSPPENQEAESSEDGEGEGIGRRCTRWWGQLVNRPFRSRYRQISP 304

Query: 311 PQDRYNSS 317
           PQDR+NSS
Sbjct: 305 PQDRHNSS 312

[Close]

BLAST of Tan0012317 vs. NCBI nr
Match: KAG7031679.1 (E3 ubiquitin-protein ligase RZF1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 521.5 bits (1342), Expect = 4.9e-144
Identity = 250/309 (80.91%), Postives = 272/309 (88.03%), Query Frame = 0


Query: 11  NNRHT--TTFHLYWCYQCHRMVTLA-AADPSELFCPRCSGQFLEELHLTRPELHPSPETR 70
           ++RHT  ++FH YWC+QCHRMVTLA   DPSEL C RCSGQFLEELHLTR +   SPETR
Sbjct: 5   SSRHTRSSSFHFYWCHQCHRMVTLATTGDPSELICSRCSGQFLEELHLTRRQFDNSPETR 64

Query: 71  LLEALSLMLDPPVRLFRLPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGRTRRRTRSMD 130
           LLEALSLMLDPPVRLFRLPD +RRRP LHQFD+ DR+ FSD EGDEWFH RTRRR RS+D
Sbjct: 65  LLEALSLMLDPPVRLFRLPDIDRRRPMLHQFDNFDRQSFSDPEGDEWFHWRTRRRNRSLD 124

Query: 131 ERADSGLQPPNPNRSRTVIILRPTDPSRPLQPIIPTRADPRDYFTGPQLDELIEELTQND 190
           ER +S LQPPN NRSRTVIILRPTDPSRP+QPIIP RADPRDYFTGP+LDELIEELTQND
Sbjct: 125 ERENSSLQPPNQNRSRTVIILRPTDPSRPVQPIIPPRADPRDYFTGPRLDELIEELTQND 184

Query: 191 RPGPPPANEAAIEGIPTVKIEAEHLKNDSMCPVCKEEFEVGGEARELSCKHIYHSECIVP 250
           RPGP PA+EAAIE IPTVKI  EHLKND+MCPVCKEEFE+G EAREL CKH+YHSECIVP
Sbjct: 185 RPGPQPASEAAIERIPTVKIATEHLKNDTMCPVCKEEFEIGEEARELMCKHVYHSECIVP 244

Query: 251 WLRLHNSCPVCRQEMRPSPPENQAAETESSEDGEGIGRRCARWWGQLVSRPFRNRYRQLS 310
           WLRLHNSCPVCRQEM PSPPENQ AE+    +GEGIGRRC RWWGQLV+RP R+RYRQLS
Sbjct: 245 WLRLHNSCPVCRQEMPPSPPENQEAESSEDGEGEGIGRRCTRWWGQLVNRPSRSRYRQLS 304

Query: 311 PPQDRYNSS 317
           PPQDR+NSS
Sbjct: 305 PPQDRHNSS 313

[Close]

BLAST of Tan0012317 vs. NCBI nr
Match: XP_022937137.1 (E3 ubiquitin-protein ligase RZF1-like [Cucurbita moschata] >XP_022937138.1 E3 ubiquitin-protein ligase RZF1-like [Cucurbita moschata])

HSP 1 Score: 493.8 bits (1270), Expect = 1.1e-135
Identity = 241/321 (75.08%), Postives = 271/321 (84.42%), Query Frame = 0


Query: 1   MSLSPPRQRTNN-RHTTTFHLYWCYQCHRMVTLAAADPSELFCPRCSGQFLEELHLTRPE 60
           M+LSPP + TN+ RH  TFHLYWC+QCHR VTLA+A+PS+L CPRC G F+EE+HL  PE
Sbjct: 1   MALSPPPRTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLPE 60

Query: 61  LHPSPETRLLEALSLMLDPPVRLF--RLPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHG 120
           L PSPETRLLEALSLMLDPPVRLF  R PD +RRRPPLH+FDD DRR FSD EGDEW H 
Sbjct: 61  LDPSPETRLLEALSLMLDPPVRLFNYRHPDPDRRRPPLHRFDDFDRRSFSDPEGDEWLHW 120

Query: 121 RTRRRTRSMDERADSGLQPPNPNRSRTVIILRPTDPSRPLQPIIPTRADPRDYFTGPQLD 180
           RTRRR+RS+DER +SG QPPNPN+SRTVI+L P D  +P+QPIIP RA+PRDYFTGP+LD
Sbjct: 121 RTRRRSRSLDERDNSGRQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYFTGPRLD 180

Query: 181 ELIEELTQNDRPGPPPANEAAIEGIPTVKIEAEHLKNDSMCPVCKEEFEVGGEARELSCK 240
           ELIE+LTQNDRPGPPPA E AIE IPTVKI AEHLKNDS CPVCKEEFEVGGEARELSCK
Sbjct: 181 ELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSQCPVCKEEFEVGGEARELSCK 240

Query: 241 HIYHSECIVPWLRLHNSCPVCRQEMRPSPPENQAAETESSEDGEGIGRRCARWWGQLVSR 300
           H+YHSECIVPWLRLHNSCPVCRQE+ P PPEN AA   +SE+G  IGR C RWW +L S 
Sbjct: 241 HLYHSECIVPWLRLHNSCPVCRQEIPPLPPENGAA--GNSENGVRIGRACTRWWNRLASW 300

Query: 301 PFRNRYRQLSP--PQDRYNSS 317
            FRNRYRQ+SP  P +R+N+S
Sbjct: 301 LFRNRYRQISPSSPPNRFNAS 319

[Close]

BLAST of Tan0012317 vs. ExPASy TrEMBL
Match: A0A6J1FTR5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446755 PE=4 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 5.7e-146
Identity = 255/308 (82.79%), Postives = 276/308 (89.61%), Query Frame = 0


Query: 11  NNRHT-TTFHLYWCYQCHRMVTLAAA-DPSELFCPRCSGQFLEELHLTRPELHPSPETRL 70
           ++RHT ++FH YWC+QCHRMVTLA   DPSEL C RCSGQFLEELHLTRP+   SPETRL
Sbjct: 5   SSRHTRSSFHFYWCHQCHRMVTLATTRDPSELICSRCSGQFLEELHLTRPQFDNSPETRL 64

Query: 71  LEALSLMLDPPVRLFRLPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGRTRRRTRSMDE 130
           LEALSLMLDPPVRLFRLPD +RRRP LHQFD+ DR+ FSD EGDEWFH RTRRR RS+DE
Sbjct: 65  LEALSLMLDPPVRLFRLPDIDRRRPMLHQFDNFDRQSFSDPEGDEWFHWRTRRRNRSLDE 124

Query: 131 RADSGLQPPNPNRSRTVIILRPTDPSRPLQPIIPTRADPRDYFTGPQLDELIEELTQNDR 190
           R +S LQPPN NRSRTVIILRPTDPSRP+QPIIP RADPRDYFTGP+LDELIEELTQNDR
Sbjct: 125 RDNSSLQPPNQNRSRTVIILRPTDPSRPVQPIIPPRADPRDYFTGPRLDELIEELTQNDR 184

Query: 191 PGPPPANEAAIEGIPTVKIEAEHLKNDSMCPVCKEEFEVGGEARELSCKHIYHSECIVPW 250
           PGP PA+EAAIE IPTVKI AEHLKND+MCPVCKEEFE+G EAREL CKH+YHSECIVPW
Sbjct: 185 PGPQPASEAAIERIPTVKIAAEHLKNDTMCPVCKEEFEIGEEARELMCKHVYHSECIVPW 244

Query: 251 LRLHNSCPVCRQEMRPSPPENQAAETESSEDGEGIGRRCARWWGQLVSRPFRNRYRQLSP 310
           LRLHNSCPVCRQEM PSPPENQAA  ESS+D EGIGRRC RWWGQLV+RPFR+RYRQLSP
Sbjct: 245 LRLHNSCPVCRQEMPPSPPENQAA--ESSDDEEGIGRRCTRWWGQLVNRPFRSRYRQLSP 304

Query: 311 PQDRYNSS 317
           PQD +NSS
Sbjct: 305 PQDSHNSS 310

[Close]

BLAST of Tan0012317 vs. ExPASy TrEMBL
Match: A0A6J1ISV3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111480302 PE=4 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 257/308 (83.44%), Postives = 274/308 (88.96%), Query Frame = 0


Query: 11  NNRHT-TTFHLYWCYQCHRMVTLA-AADPSELFCPRCSGQFLEELHLTRPELHPSPETRL 70
           ++RHT  +FHLYWC+QCHRMVTLA A DPSEL C RCSGQFLEELHLTRP+   SPETRL
Sbjct: 5   SSRHTRPSFHLYWCHQCHRMVTLATAGDPSELICSRCSGQFLEELHLTRPQFDNSPETRL 64

Query: 71  LEALSLMLDPPVRLFRLPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGRTRRRTRSMDE 130
           LEALSLMLDPPVRLFRLPD +RRRP  HQFD+ DRR FSD EGDEWFHGRTRRR RS+DE
Sbjct: 65  LEALSLMLDPPVRLFRLPDLDRRRPMSHQFDNFDRRSFSDPEGDEWFHGRTRRRNRSLDE 124

Query: 131 RADSGLQPPNPNRSRTVIILRPTDPSRPLQPIIPTRADPRDYFTGPQLDELIEELTQNDR 190
           R +S L PPN NRSRT IILRPTDPSRP+Q IIP RADP DYFTGP+LDELIEELTQNDR
Sbjct: 125 RDNSSLLPPNQNRSRTFIILRPTDPSRPVQTIIPPRADPGDYFTGPRLDELIEELTQNDR 184

Query: 191 PGPPPANEAAIEGIPTVKIEAEHLKNDSMCPVCKEEFEVGGEARELSCKHIYHSECIVPW 250
           PGP PA+EAAIE IPTVKI AEHLKNDSMCPVCKEEFE+G EAREL CKH+YHSECIVPW
Sbjct: 185 PGPQPASEAAIERIPTVKIAAEHLKNDSMCPVCKEEFEIGEEARELMCKHVYHSECIVPW 244

Query: 251 LRLHNSCPVCRQEMRPSPPENQAAETESSEDGEGIGRRCARWWGQLVSRPFRNRYRQLSP 310
           LRLHNSCPVCRQEM PSPPENQAA  ESSED EGIGRRC RWWGQLV+RPFR+RYRQLSP
Sbjct: 245 LRLHNSCPVCRQEMPPSPPENQAA--ESSEDEEGIGRRCTRWWGQLVNRPFRSRYRQLSP 304

Query: 311 PQDRYNSS 317
           PQDR+NSS
Sbjct: 305 PQDRHNSS 310

[Close]

BLAST of Tan0012317 vs. ExPASy TrEMBL
Match: A0A6J1FAA6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111443524 PE=4 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 5.3e-136
Identity = 241/321 (75.08%), Postives = 271/321 (84.42%), Query Frame = 0


Query: 1   MSLSPPRQRTNN-RHTTTFHLYWCYQCHRMVTLAAADPSELFCPRCSGQFLEELHLTRPE 60
           M+LSPP + TN+ RH  TFHLYWC+QCHR VTLA+A+PS+L CPRC G F+EE+HL  PE
Sbjct: 1   MALSPPPRTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLPE 60

Query: 61  LHPSPETRLLEALSLMLDPPVRLF--RLPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHG 120
           L PSPETRLLEALSLMLDPPVRLF  R PD +RRRPPLH+FDD DRR FSD EGDEW H 
Sbjct: 61  LDPSPETRLLEALSLMLDPPVRLFNYRHPDPDRRRPPLHRFDDFDRRSFSDPEGDEWLHW 120

Query: 121 RTRRRTRSMDERADSGLQPPNPNRSRTVIILRPTDPSRPLQPIIPTRADPRDYFTGPQLD 180
           RTRRR+RS+DER +SG QPPNPN+SRTVI+L P D  +P+QPIIP RA+PRDYFTGP+LD
Sbjct: 121 RTRRRSRSLDERDNSGRQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYFTGPRLD 180

Query: 181 ELIEELTQNDRPGPPPANEAAIEGIPTVKIEAEHLKNDSMCPVCKEEFEVGGEARELSCK 240
           ELIE+LTQNDRPGPPPA E AIE IPTVKI AEHLKNDS CPVCKEEFEVGGEARELSCK
Sbjct: 181 ELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSQCPVCKEEFEVGGEARELSCK 240

Query: 241 HIYHSECIVPWLRLHNSCPVCRQEMRPSPPENQAAETESSEDGEGIGRRCARWWGQLVSR 300
           H+YHSECIVPWLRLHNSCPVCRQE+ P PPEN AA   +SE+G  IGR C RWW +L S 
Sbjct: 241 HLYHSECIVPWLRLHNSCPVCRQEIPPLPPENGAA--GNSENGVRIGRACTRWWNRLASW 300

Query: 301 PFRNRYRQLSP--PQDRYNSS 317
            FRNRYRQ+SP  P +R+N+S
Sbjct: 301 LFRNRYRQISPSSPPNRFNAS 319

[Close]

BLAST of Tan0012317 vs. ExPASy TrEMBL
Match: A0A6J1IKH7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111476446 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.5e-135
Identity = 242/321 (75.39%), Postives = 269/321 (83.80%), Query Frame = 0


Query: 1   MSLSPPRQRTNN-RHTTTFHLYWCYQCHRMVTLAAADPSELFCPRCSGQFLEELHLTRPE 60
           M+LSPP   TN+ RH  TFHLYWC+QCHR VTLA+A+PS+L CPRC G F+EE+HL  PE
Sbjct: 1   MALSPPPHTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLPE 60

Query: 61  LHPSPETRLLEALSLMLDPPVRLF--RLPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHG 120
           L PSPETRLLEALSLMLDPPVRLF  R PD +RRRPPLH+FDD DRR FSD EGDEW H 
Sbjct: 61  LDPSPETRLLEALSLMLDPPVRLFNYRHPDPDRRRPPLHRFDDFDRRSFSDPEGDEWLHW 120

Query: 121 RTRRRTRSMDERADSGLQPPNPNRSRTVIILRPTDPSRPLQPIIPTRADPRDYFTGPQLD 180
           RTRRR+RS+DER +SG QPPNPN+SRTVI+L P D  +P+QPIIP RA+PRDYFTGP+LD
Sbjct: 121 RTRRRSRSLDERDNSGRQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYFTGPRLD 180

Query: 181 ELIEELTQNDRPGPPPANEAAIEGIPTVKIEAEHLKNDSMCPVCKEEFEVGGEARELSCK 240
           ELIE+LTQNDRPGPPPA E AIE IPTVKI AEHLKNDS CPVCKEEFEVGGEARELSCK
Sbjct: 181 ELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSHCPVCKEEFEVGGEARELSCK 240

Query: 241 HIYHSECIVPWLRLHNSCPVCRQEMRPSPPENQAAETESSEDGEGIGRRCARWWGQLVSR 300
           HIYHSECIVPWLRLHNSCPVCRQE+ P PPEN AA   +SE    IGR C RWW +LVS 
Sbjct: 241 HIYHSECIVPWLRLHNSCPVCRQEIPPLPPENGAA--GNSETEVRIGRACTRWWNRLVSW 300

Query: 301 PFRNRYRQLSP--PQDRYNSS 317
            FRNRYRQ+SP  P +R+N+S
Sbjct: 301 LFRNRYRQISPSSPPNRFNAS 319

[Close]

BLAST of Tan0012317 vs. ExPASy TrEMBL
Match: A0A0A0L0L2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G370570 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.4e-128
Identity = 228/319 (71.47%), Postives = 261/319 (81.82%), Query Frame = 0


Query: 1   MSLSPPRQRTNNRHTTTFHLYWCYQCHRMVTLAAADPSELFCPRCSGQFLEELHLTRPEL 60
           MSL+PP+  TNNRHTTTF LYWC+QCHR VTLA+A PSEL CPRC GQF+EE+ LT PE 
Sbjct: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF 60

Query: 61  HPSPETRLLEALSLMLDPPVRLF--RLPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGR 120
           +PSPE RL EALSLML+ P+R+F  R P+ NR  PP H+F++ DRR FSD EGDE    R
Sbjct: 61  NPSPEGRLFEALSLMLNQPIRIFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR 120

Query: 121 TRRRTRSMDERADSGLQPPNPNRSRTVIILRPTDPSRPLQPIIPTRADPRDYFTGPQLDE 180
            R R+RS+DER + G QPPNPNRSRTVI+  P D  +P+QPI+P R  PRDYFTGPQLDE
Sbjct: 121 RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPILPRRISPRDYFTGPQLDE 180

Query: 181 LIEELTQNDRPGPPPANEAAIEGIPTVKIEAEHLKNDSMCPVCKEEFEVGGEARELSCKH 240
           LIEELTQNDRPGP PA+E AIE IPTVKIEAEHLKN+S CPVCKEEFEVGGEARELSCKH
Sbjct: 181 LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH 240

Query: 241 IYHSECIVPWLRLHNSCPVCRQEMRPSPPENQAAETESSEDGEGIGRRCARWWGQLVSRP 300
           IYHSECIVPWLRLHNSCPVCRQEM    PEN+A+   SS + EG+GRRCARWW  LV  P
Sbjct: 241 IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEAS---SSSEEEGMGRRCARWWSHLVPWP 300

Query: 301 FRNRYRQLSP-PQDRYNSS 317
           FRNRYRQ+SP P++R+++S
Sbjct: 301 FRNRYRQISPFPRNRFDAS 316

[Close]

BLAST of Tan0012317 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 231.1 bits (588), Expect = 1.2e-60
Identity = 151/343 (44.02%), Postives = 192/343 (55.98%), Query Frame = 0


Query: 1   MSLSPPRQRTN---NRHTTTFHLYWCYQCHRMVTLAAADPSELFCPRCSGQFLEEL---- 60
           MSLS P  RT+   N    TF LYWCY C RMV +A+++PSE+ CPRC  QF+ E+    
Sbjct: 1   MSLSQPITRTDSAPNGAFRTFGLYWCYHCDRMVRIASSNPSEIACPRCLRQFVVEIETRQ 60

Query: 61  -------HLTRPELHPSPETRLLEALSLMLDPPVRLFRLPDSNRRRPPLHQFDDVDRRPF 120
                  H T P    SPE RLLEALSLM + P  + R       R       + + RP 
Sbjct: 61  RPRFTFNHAT-PPFDASPEARLLEALSLMFE-PATIGRFGADPFLRARSRNILEPESRPR 120

Query: 121 SDLEGDEWFHGRTRRRTRSMDERADSGLQPPNPNRSRTVIILRPTDPSRPLQPII--PTR 180
                         RR  S+D   + GL  P     RT +ILRP +P+ PL  II  P +
Sbjct: 121 PQ-----------HRRRHSLDNVNNGGLPLPR----RTYVILRPNNPTSPLGNIIAPPNQ 180

Query: 181 ADPR-----DYFTG-PQLDELIEELTQNDRPGPPPANEAAIEGIPTVKIEAEHLKND-SM 240
           A PR     DYFTG   L++LIE+LTQ+DRPGPPPA+E  I  +P+VKI  +HL ND S 
Sbjct: 181 APPRHVNSHDYFTGASSLEQLIEQLTQDDRPGPPPASEPTINSLPSVKITPQHLTNDMSQ 240

Query: 241 CPVCKEEFEVGGEARELSCKHIYHSECIVPWLRLHNSCPVCRQEMRPSPPENQAAETESS 300
           C VC EEF VGG+A EL CKHIYH +CIVPWLRL+NSCP+CR+++   P  N  AE+   
Sbjct: 241 CTVCMEEFIVGGDATELPCKHIYHKDCIVPWLRLNNSCPICRRDL---PLVNTVAESRER 300

Query: 301 ED---GEGIGRRCARWWGQLVSRPFRNRYRQLSPPQDRYNSSR 318
            +    +   RR  RW       PFR RY+++SP +    + R
Sbjct: 301 SNPIRQDMPERRRPRWMQLGNIWPFRARYQRVSPEETANQNPR 323

[Close]

BLAST of Tan0012317 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 142.1 bits (357), Expect = 7.6e-34
Identity = 94/297 (31.65%), Postives = 153/297 (51.52%), Query Frame = 0


Query: 20  LYWCYQCHRMVTLAAADPSELFCPRCSGQFLEELHLTRPELHPSPETRLLEALSLMLDPP 79
           +++CYQC++ VT++ +  ++ FCP C+  FLEE     P    +      ++   M DP 
Sbjct: 20  MFFCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSSDSFFPMADPF 79

Query: 80  VRLFRLPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGRTRRRTRSMDERADSGLQPPNP 139
             L  L   +    P            S ++    F    + + RS  +   S    P  
Sbjct: 80  STLLPLIFGSSAAAP------------SGMDFMSLFGPSMQPQARSTQQNPQSDAFDPFT 139

Query: 140 NRSRTVIILRPTD-------PSRPLQPIIPTRADPRDYFTGPQLDELIEELTQND--RPG 199
                +  LR +         + P  P      +  DYF GP L++LI++L +ND  R G
Sbjct: 140 FLQNHLQTLRSSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYG 199

Query: 200 PPPANEAAIEGIPTVKIEAEHLKND-SMCPVCKEEFEVGGEARELSCKHIYHSECIVPWL 259
            PPA+++AI+ +PTVK+  + LK++ + C VC +EFE G + +++ CKH++H +C++PWL
Sbjct: 200 TPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWL 259

Query: 260 RLHNSCPVCRQEMRPSPP--ENQAAETESSEDGEGI--GRRCARWWGQLVSRPFRNR 303
            LHNSCPVCR E+    P  EN++  ++ S DG+G   G++  R+  QL   PFR +
Sbjct: 260 ELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQGSVEGQQTPRFSIQL-PWPFRRQ 303

[Close]

BLAST of Tan0012317 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 138.3 bits (347), Expect = 1.1e-32
Identity = 99/294 (33.67%), Postives = 137/294 (46.60%), Query Frame = 0


Query: 21  YWCYQCHRMVTLAAADPSELFCPRCSGQFLEELHLTRPELHPSPETRLLEALSLMLDPPV 80
           +WC++C R V L   +P    C  C G F+EEL + +                     P 
Sbjct: 8   HWCHRCQRAVRLHGQEP---VCFYCGGGFVEELDMAQAS-------------------PF 67

Query: 81  RLFR----LPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGRTRRRTRSMDERADSGLQP 140
            +FR    + + ++    +  F    R   ++   D    G    RT S       GL P
Sbjct: 68  DMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRG----RTISSGPENFPGLAP 127

Query: 141 PNPNRSRTVIILRPTDPSRPL-----QPIIPTRADPRDYFTGPQLDELIEELTQ-NDRPG 200
                 +    L   +    L       I  TR +  DYF GP L+EL E+L+    R G
Sbjct: 128 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 187

Query: 201 PPPANEAAIEGIPTVKIEAEHLK-NDSMCPVCKEEFEVGGEARELSCKHIYHSECIVPWL 260
           PPPA  +AI+ +PT+KI   HL+ +DS CPVCK+EFE+G EA+++ C HIYHS+CIVPWL
Sbjct: 188 PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWL 247

Query: 261 RLHNSCPVCRQEM----RPSPPENQAAETESSEDGEGIGRRCARWWGQLVSRPF 300
             HNSCPVCRQE+     PS  +N+   T +           +R  G     PF
Sbjct: 248 VQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPF 275

[Close]

BLAST of Tan0012317 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 138.3 bits (347), Expect = 1.1e-32
Identity = 99/294 (33.67%), Postives = 137/294 (46.60%), Query Frame = 0


Query: 21  YWCYQCHRMVTLAAADPSELFCPRCSGQFLEELHLTRPELHPSPETRLLEALSLMLDPPV 80
           +WC++C R V L   +P    C  C G F+EEL + +                     P 
Sbjct: 8   HWCHRCQRAVRLHGQEP---VCFYCGGGFVEELDMAQAS-------------------PF 67

Query: 81  RLFR----LPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGRTRRRTRSMDERADSGLQP 140
            +FR    + + ++    +  F    R   ++   D    G    RT S       GL P
Sbjct: 68  DMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRG----RTISSGPENFPGLAP 127

Query: 141 PNPNRSRTVIILRPTDPSRPL-----QPIIPTRADPRDYFTGPQLDELIEELTQ-NDRPG 200
                 +    L   +    L       I  TR +  DYF GP L+EL E+L+    R G
Sbjct: 128 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 187

Query: 201 PPPANEAAIEGIPTVKIEAEHLK-NDSMCPVCKEEFEVGGEARELSCKHIYHSECIVPWL 260
           PPPA  +AI+ +PT+KI   HL+ +DS CPVCK+EFE+G EA+++ C HIYHS+CIVPWL
Sbjct: 188 PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWL 247

Query: 261 RLHNSCPVCRQEM----RPSPPENQAAETESSEDGEGIGRRCARWWGQLVSRPF 300
             HNSCPVCRQE+     PS  +N+   T +           +R  G     PF
Sbjct: 248 VQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPF 275

[Close]

BLAST of Tan0012317 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 138.3 bits (347), Expect = 1.1e-32
Identity = 99/294 (33.67%), Postives = 137/294 (46.60%), Query Frame = 0


Query: 21  YWCYQCHRMVTLAAADPSELFCPRCSGQFLEELHLTRPELHPSPETRLLEALSLMLDPPV 80
           +WC++C R V L   +P    C  C G F+EEL + +                     P 
Sbjct: 8   HWCHRCQRAVRLHGQEP---VCFYCGGGFVEELDMAQAS-------------------PF 67

Query: 81  RLFR----LPDSNRRRPPLHQFDDVDRRPFSDLEGDEWFHGRTRRRTRSMDERADSGLQP 140
            +FR    + + ++    +  F    R   ++   D    G    RT S       GL P
Sbjct: 68  DMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRG----RTISSGPENFPGLAP 127

Query: 141 PNPNRSRTVIILRPTDPSRPL-----QPIIPTRADPRDYFTGPQLDELIEELTQ-NDRPG 200
                 +    L   +    L       I  TR +  DYF GP L+EL E+L+    R G
Sbjct: 128 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 187

Query: 201 PPPANEAAIEGIPTVKIEAEHLK-NDSMCPVCKEEFEVGGEARELSCKHIYHSECIVPWL 260
           PPPA  +AI+ +PT+KI   HL+ +DS CPVCK+EFE+G EA+++ C HIYHS+CIVPWL
Sbjct: 188 PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWL 247

Query: 261 RLHNSCPVCRQEM----RPSPPENQAAETESSEDGEGIGRRCARWWGQLVSRPF 300
             HNSCPVCRQE+     PS  +N+   T +           +R  G     PF
Sbjct: 248 VQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPF 275

The following BLAST results are available for this feature:

BLAST of Tan0012317 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AK4	7.4e-34	35.64	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]
Q8LPN7	1.1e-32	31.65	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
O22197	1.5e-31	33.67	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
P0CH30	1.5e-31	34.15	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q9SNB6	1.3e-25	31.08	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]

BLAST of Tan0012317 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022941465.1	1.2e-145	82.79	E3 ubiquitin-protein ligase RZF1-like [Cucurbita moschata]	[more]
XP_022981032.1	2.6e-145	83.44	E3 ubiquitin-protein ligase RZF1-like [Cucurbita maxima]	[more]
XP_023525796.1	1.0e-144	81.17	E3 ubiquitin-protein ligase RZF1-like [Cucurbita pepo subsp. pepo]	[more]
KAG7031679.1	4.9e-144	80.91	E3 ubiquitin-protein ligase RZF1, partial [Cucurbita argyrosperma subsp. argyros...	[more]
XP_022937137.1	1.1e-135	75.08	E3 ubiquitin-protein ligase RZF1-like [Cucurbita moschata] >XP_022937138.1 E3 ub...	[more]

BLAST of Tan0012317 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FTR5	5.7e-146	82.79	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446755...	[more]
A0A6J1ISV3	1.3e-145	83.44	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111480302 P...	[more]
A0A6J1FAA6	5.3e-136	75.08	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111443524...	[more]
A0A6J1IKH7	3.5e-135	75.39	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111476446 P...	[more]
A0A0A0L0L2	2.4e-128	71.47	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G370570 PE...	[more]

BLAST of Tan0012317 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G60360.1	1.2e-60	44.02	RING/U-box superfamily protein	[more]
AT3G19950.1	7.6e-34	31.65	RING/U-box superfamily protein	[more]
AT2G40830.2	1.1e-32	33.67	RING-H2 finger C1A	[more]
AT2G40830.1	1.1e-32	33.67	RING-H2 finger C1A	[more]
AT2G40830.3	1.1e-32	33.67	RING-H2 finger C1A	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 218..258 e-value: 1.3E-6 score: 37.9
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 217..259 e-value: 9.5E-12 score: 45.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 218..259 score: 12.135206
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 181..265 e-value: 9.6E-22 score: 78.5
IPR039525	E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon	PFAM	PF14369	zinc_ribbon_9	coord: 20..53 e-value: 1.3E-12 score: 47.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 151..170
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 261..281
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 120..144
None	No IPR available	PANTHER	PTHR15710:SF29	SUBFAMILY NOT NAMED	coord: 14..274
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 14..274
None	No IPR available	CDD	cd16667	RING-H2_RNF126_like	coord: 218..259 e-value: 2.7246E-22 score: 86.2176
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 198..265

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0012317.1	Tan0012317.1	mRNA
Tan0012317.2	Tan0012317.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0061630	ubiquitin protein ligase activity