Tan0012289 (gene) Snake gourd v1

BLAST of Tan0012289 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 2.2e-75
Identity = 195/539 (36.18%), Postives = 299/539 (55.47%), Query Frame = 0

Query: 9   QWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLFGFFSGLAA 68
           +W  LV  IW+Q+  GTNF+F +YSS LK  L ISQ++LN LA ASD GK FG+ SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 69  NYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICWINTVCYMV 128
            Y PL +VL   + +G VGYG+Q+L ITN +  P + L+F   +LAG SICW NT C+++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYS-LVFLCCLLAGLSICWFNTACFIL 162

Query: 129 AIRNFPSNRQVAVGISTSYQGLSAKIYTNIVDSVFSKRSSKTAETFLLLNSVLPLGVC-- 188
            IR+FP+NR +A+ ++ S+ G+SA +Y+      F+  +  ++  +LLLNS++PL V   
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYS----LAFNAINPSSSNLYLLLNSLVPLVVSFA 222

Query: 189 ----VLASPLARVVKGVEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSPLSGLV 248
               VL  P        + +      F ++ V+ V T    +++S  + S  L+     +
Sbjct: 223 ALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLN----FI 282

Query: 249 GILVFLFLPLIVAVVANVKETVKERKERRVYHFTVEEQKGDVEQRIDEDVERISPKNEGK 308
           G +V L  PL   ++   ++        R+ H    E  G V   IDE ++        K
Sbjct: 283 GAVVLLVFPLCAPLLVYARDYFLPVINARLNH----ESSGYVMLNIDE-LKNQKTSVSSK 342

Query: 309 LG-------KESEDEEVMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESR 368
            G       KE     + +E   + ++ R+ FWLY+  Y  G T+GL + NNLGQIA+S 
Sbjct: 343 TGYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSL 402

Query: 369 RSSSVSSLVSLSSSFGFFGRLMPSLVDHFFSRRKFMKSKPGWMVALMGAMCGGFFLLSCP 428
             +S ++LV++ SSF FFGRL+ +  D  F  ++F  ++ GW    +      FFLL+  
Sbjct: 403 GQNS-TTLVTIYSSFSFFGRLLSAAPD--FMHKRFRLTRTGWFAIALLPTPIAFFLLAVS 462

Query: 429 TNM--SLCASTAIIAVCTGAITSISVSTTTELFGANNFCINHNVVVANIPFGSFLFGYMA 488
           ++   +L  +TA+I + +G I + +VS T++LFG N+  +NHN+++ NIP GS L+GY+A
Sbjct: 463 SSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIA 522

Query: 489 AFLYHKQA-----PATGK---CIGVECYRTTFLIWGCFCSFGTLLALLLFARTTTFYSR 525
           A +Y   A     P       CIG +CY  TF+ WGC    G + +L L+ RT   Y R
Sbjct: 523 ASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHR 564

BLAST of Tan0012289 vs. NCBI nr
Match: XP_038895111.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Benincasa hispida])

HSP 1 Score: 812.4 bits (2097), Expect = 2.3e-231
Identity = 430/523 (82.22%), Postives = 466/523 (89.10%), Query Frame = 0

Query: 3   SSSRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLFGF 62
           SSS V QWLSLVGIIWLQSINGTNFNFPSYSSQLK  LS+SQLQLNNLAFASDAGKLF  
Sbjct: 2   SSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 61

Query: 63  FSGLAANYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICWIN 122
           FSGLAANYLPLWLVL IG+ LGLVGYG+QYLF+TNQ+ SP+  +IF LTVLAGNSICWIN
Sbjct: 62  FSGLAANYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWIN 121

Query: 123 TVCYMVAIRNFPSNRQVAVGISTSYQGLSAKIYTNIVDSVFSKRSSKTAETFLLLNSVLP 182
           TVCYMVAI NF +NRQ+AVGISTSYQGLSAKIYTNIV S+FSK SSKT ETFLLLNSVLP
Sbjct: 122 TVCYMVAITNFGTNRQIAVGISTSYQGLSAKIYTNIVSSIFSKHSSKTTETFLLLNSVLP 181

Query: 183 LGVCVLASPLARVVKGVEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSPLSGLV 242
           +GVCVLASPLAR++K VEEQG L+VGFFVIFVIT+ATG+FA VTSVGSIS MLSPLSGLV
Sbjct: 182 MGVCVLASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSGLV 241

Query: 243 GILVFLFLPLIVAVVANVKETVKERKERRVYHFTVEEQKGDVEQRIDEDVERISPKNEGK 302
           GI+VFL LPL+VAVV  VKE++KE KERRVYHFTVEE K         DVER+  +N+ K
Sbjct: 242 GIVVFLVLPLVVAVVEKVKESLKEGKERRVYHFTVEENK---------DVERVRGENQSK 301

Query: 303 LGKESEDEEVMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRRSSSVSS 362
           L ++ + EE MEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESR SSSVSS
Sbjct: 302 LERDGDSEEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSS 361

Query: 363 LVSLSSSFGFFGRLMPSLVDHFFSRRKFMKSKPGWMVALMGAMCGGFFLLSCPTNMSLCA 422
           LVSLSSSFGFFGRLMPS++D+F SR+K MKSKPGWMV LMG +CGGFFLL CPT++SLC 
Sbjct: 362 LVSLSSSFGFFGRLMPSILDYFLSRKKCMKSKPGWMVGLMGVLCGGFFLLLCPTDVSLCV 421

Query: 423 STAIIAVCTGAITSISVSTTTELFGANNFCINHNVVVANIPFGSFLFGYMAAFLYHKQA- 482
           STAIIAVCTGAITSISVSTT+ELFGANNF INHN++VANIPFGSFLFGYMAAFLYHKQA 
Sbjct: 422 STAIIAVCTGAITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYHKQAG 481

Query: 483 -PATGKCIGVECYRTTFLIWGCFCSFGTLLALLLFARTTTFYS 524
              TGKCIGVECYRTTF IWGCF SFGTLLALLLFART TFYS
Sbjct: 482 GDGTGKCIGVECYRTTFFIWGCFSSFGTLLALLLFARTKTFYS 515

BLAST of Tan0012289 vs. NCBI nr
Match: XP_022970645.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 793.5 bits (2048), Expect = 1.1e-225
Identity = 427/530 (80.57%), Postives = 461/530 (86.98%), Query Frame = 0

Query: 5   SRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLFGFFS 64
           S V QWLSL+GIIWLQSINGTNFNFPSYSSQLKHHLSISQ QLNNLAFASDAGKLF   S
Sbjct: 3   SSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVS 62

Query: 65  GLAANYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICWINTV 124
           GLAANYLPLW VLIIGS LGLVGYG+QYLFITNQ HSP+  LIFFLTVLAGNSICWINTV
Sbjct: 63  GLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWINTV 122

Query: 125 CYMVAIRNFPSNRQVAVGISTSYQGLSAKIYTNIVDSVFSKRSSKTAETFLLLNSVLPLG 184
           CY+VAIRNFPSNRQVAVGISTSYQGLSAKIYT+IV  +FSKRS KTAETFLL NSV PLG
Sbjct: 123 CYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLG 182

Query: 185 VCVLASPLARVVKGVEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSPLSGLVGI 244
           VCVL SP+AR+V+ VEEQG+L+VGFFVIFVIT+ATGVFAVVTSVGSIS MLSPLSGLVGI
Sbjct: 183 VCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVGI 242

Query: 245 LVFLFLPLIVAVVANVKETVKERKERRVYHFTVEEQKGDVEQRIDEDVERISPKNEGKLG 304
           LVFL LP++V VV +VK++V+E KERRVYH T+EE              RI P+NE KL 
Sbjct: 243 LVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED------------TRIRPENEAKLN 302

Query: 305 KESEDEEVMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRRSSSVSSLV 364
           ++  D E MEEIGA EM+KR+NFWLY GVY FGATLGLAFLNNLGQIAESR S SVSSLV
Sbjct: 303 RDGGD-EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLV 362

Query: 365 SLSSSFGFFGRLMPSLVDHFFSRRKFMKSKPGWMVALMGAMCGGFFLLSCPTNMSLCAST 424
           SLSSSFGFFGRLMPSL+DHF SRRK MKSKPGW+VALMGAMCGGFFLLS PT++ LC ST
Sbjct: 363 SLSSSFGFFGRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVST 422

Query: 425 AIIAVCTGAITSISVSTTTELFGANNFCINHNVVVANIPFGSFLFGYMAAFLYHKQAP-A 484
           A+IAVCTGAITSISVSTT+ELFGANNF INHNVVVANIP GSFLFGYMAA+LY KQA   
Sbjct: 423 AVIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGG 482

Query: 485 TGKCIGVECYRTTFLIWGCFCSFGTLLALLLFARTTTFYS-RKSPMPEHS 533
            GKCIGVECYRTTF+IWGC CSFGTLLALLLFART +FYS  KSP  + S
Sbjct: 483 AGKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGS 519

BLAST of Tan0012289 vs. NCBI nr
Match: KAG6583327.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019101.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 783.5 bits (2022), Expect = 1.2e-222
Identity = 423/531 (79.66%), Postives = 460/531 (86.63%), Query Frame = 0

Query: 5   SRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLFGFFS 64
           S V QWLSL+GIIWLQSINGTNFNFPSYSSQLKHHLSISQ QLNNLAFASDAGKLF   S
Sbjct: 3   SSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVS 62

Query: 65  GLAANYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICWINTV 124
           GLAANYLPLW +LIIGS LGLVGYG+QYLFITNQ HSP+  LIFFLTVLAGNSICWINTV
Sbjct: 63  GLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWINTV 122

Query: 125 CYMVAIRNFPSNRQVAVGISTSYQGLSAKIYTNIVDSVFSKRSSKTAETFLLLNSVLPLG 184
           CY+VAIRNFPSNRQVAVGISTSYQGLSAKIYT+IV  +FSKRS KTAETFLL NSV PLG
Sbjct: 123 CYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLG 182

Query: 185 VCVLASPLARVVKGVEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSPLSGLVGI 244
           VCVL  P+AR+V+ VEE+G+L+VGFFVIFVIT+ATGVFAVVTSVGSIS MLSPLSGLVGI
Sbjct: 183 VCVLVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVGI 242

Query: 245 LVFLFLPLIVAVVANVKETVKERKERRVYHFTVEEQKGDVEQRIDEDVERISPKNEGKLG 304
           LVFL LP++V VV +VK++V+E KERRVYH T+EE              RI P+NE KL 
Sbjct: 243 LVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED------------TRIRPENEAKLN 302

Query: 305 KESEDEEVMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRRSSSVSSLV 364
           ++  D E MEEIGA EM+KR+NFWLY GVY FGATLGLAFLNNLGQIAESR S S SSLV
Sbjct: 303 RDGGD-EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESASSLV 362

Query: 365 SLSSSFGFFGRLMPSLVDHFFSRRKFMKSKPGWMVALMGAMCGG-FFLLSCPTNMSLCAS 424
           SLSSSFGFFGRLMPSL+DHF SRRK MKSKPGW+VALMGAMCGG FFLLS PT++ LC S
Sbjct: 363 SLSSSFGFFGRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVS 422

Query: 425 TAIIAVCTGAITSISVSTTTELFGANNFCINHNVVVANIPFGSFLFGYMAAFLYHKQAPA 484
           TA+IAVCTGAITSISVSTT+ELFGANNF INHNVVVANIPFGSFLFGYMAA+LY KQA  
Sbjct: 423 TAVIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQARG 482

Query: 485 -TGKCIGVECYRTTFLIWGCFCSFGTLLALLLFARTTTFYS-RKSPMPEHS 533
             GKCIGVECYRT+F+IWGC CSFGTLLALLLFART +FYS  KSP  + S
Sbjct: 483 DAGKCIGVECYRTSFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGS 520

BLAST of Tan0012289 vs. NCBI nr
Match: XP_023519273.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 781.9 bits (2018), Expect = 3.4e-222
Identity = 420/529 (79.40%), Postives = 455/529 (86.01%), Query Frame = 0

Query: 5   SRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLFGFFS 64
           S V QWLSL+GIIWLQSINGTNFNFPSYSSQLKHHLSISQ QLNNLAFASDAGKLF   S
Sbjct: 3   SSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVS 62

Query: 65  GLAANYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICWINTV 124
           GLAANYLPLW +LIIGS LGLVGYG+QYLFITNQ HSP+  LIFFLTVLAGNSICWINTV
Sbjct: 63  GLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWINTV 122

Query: 125 CYMVAIRNFPSNRQVAVGISTSYQGLSAKIYTNIVDSVFSKRSSKTAETFLLLNSVLPLG 184
           CY+VAIRNFPSNRQVAVGISTSYQGLSAKIYT+IV  +F+KRS KTAETFLL NSV PLG
Sbjct: 123 CYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFTKRSDKTAETFLLSNSVFPLG 182

Query: 185 VCVLASPLARVVKGVEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSPLSGLVGI 244
           VCVL  P+AR+V+ VEE+G+L VGFFVIFVIT+ATGVFAVVTSVGSIS MLSPLSGLVGI
Sbjct: 183 VCVLVCPMARIVELVEEKGNLGVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVGI 242

Query: 245 LVFLFLPLIVAVVANVKETVKERKERRVYHFTVEEQKGDVEQRIDEDVERISPKNEGKLG 304
           LVFL LP++V VV +VK++V+E KERRVYH T+EE              RI P+NE KL 
Sbjct: 243 LVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED------------TRIRPENEAKLN 302

Query: 305 KESEDEEVMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRRSSSVSSLV 364
           ++  D + MEEIGA+EMV R+NFWLY GVY FGATLGLAFLNNLGQIAESR S SVSSLV
Sbjct: 303 RDGGD-DAMEEIGAREMVTRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLV 362

Query: 365 SLSSSFGFFGRLMPSLVDHFFSRRKFMKSKPGWMVALMGAMCGG-FFLLSCPTNMSLCAS 424
           SLSSSFGFFGRLMPSL+DHF SRRK MKSKPGWMV LMGAMCGG FFLLS PT++ LC S
Sbjct: 363 SLSSSFGFFGRLMPSLLDHFLSRRKMMKSKPGWMVGLMGAMCGGFFFLLSGPTDLILCVS 422

Query: 425 TAIIAVCTGAITSISVSTTTELFGANNFCINHNVVVANIPFGSFLFGYMAAFLYHKQAP- 484
           TA+IAVCTGAITSISVSTT+ELFGANNF INHNVVVANIPFGSFLFGYMAA+LY KQA  
Sbjct: 423 TAVIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATG 482

Query: 485 ATGKCIGVECYRTTFLIWGCFCSFGTLLALLLFARTTTFYSRKSPMPEH 532
             GKCIGVECYRTTF+IWGC CSFGTLLALLLFART +FYS       H
Sbjct: 483 GAGKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSQMAH 518

BLAST of Tan0012289 vs. NCBI nr
Match: XP_022964928.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 781.9 bits (2018), Expect = 3.4e-222
Identity = 423/531 (79.66%), Postives = 460/531 (86.63%), Query Frame = 0

Query: 5   SRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLFGFFS 64
           S V QWLSL+GIIWLQSINGTNFNFPSYSS LKHHLSISQ QLNNLAFASDAGKLF   S
Sbjct: 57  SSVFQWLSLLGIIWLQSINGTNFNFPSYSSPLKHHLSISQFQLNNLAFASDAGKLFACVS 116

Query: 65  GLAANYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICWINTV 124
           GLAANYLPLW +LIIGS LGLVGYG+QYLFITNQ HSP+  LIFFL+VLAGNSICWINTV
Sbjct: 117 GLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLSVLAGNSICWINTV 176

Query: 125 CYMVAIRNFPSNRQVAVGISTSYQGLSAKIYTNIVDSVFSKRSSKTAETFLLLNSVLPLG 184
           CY+VAIRNFPSNRQVAVGISTSYQGLSAKIYT+IV  +FSKRS KTAETFLL NSV PLG
Sbjct: 177 CYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLG 236

Query: 185 VCVLASPLARVVKGVEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSPLSGLVGI 244
           VCVL  P+AR+V+ VEE+G+L+VGFFVIFVIT+ATGVFAVVTSVGSIS MLSPLSGLVGI
Sbjct: 237 VCVLVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVGI 296

Query: 245 LVFLFLPLIVAVVANVKETVKERKERRVYHFTVEEQKGDVEQRIDEDVERISPKNEGKLG 304
           LVFL LP++V VV +VK++V+E KERRVYH T+EE              RI P+NE KL 
Sbjct: 297 LVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED------------TRIRPENEAKLN 356

Query: 305 KESEDEEVMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRRSSSVSSLV 364
           ++  D E MEEIGA EM+KR+NFWLY GVY FGATLGLAFLNNLGQIAES  S SVSSLV
Sbjct: 357 RDGGD-EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESWGSESVSSLV 416

Query: 365 SLSSSFGFFGRLMPSLVDHFFSRRKFMKSKPGWMVALMGAMCGG-FFLLSCPTNMSLCAS 424
           SLSSSFGFFGRLMPSL+DHF SRRK MKSKPGW+VALMGAMCGG FFLLS PT++ LC S
Sbjct: 417 SLSSSFGFFGRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVS 476

Query: 425 TAIIAVCTGAITSISVSTTTELFGANNFCINHNVVVANIPFGSFLFGYMAAFLYHKQAPA 484
           TA+IAVCTGAITSISVSTT+ELFGANNF INHNVVVANIPFGSFLFGYMAA+LY KQA  
Sbjct: 477 TAVIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATG 536

Query: 485 -TGKCIGVECYRTTFLIWGCFCSFGTLLALLLFARTTTFYS-RKSPMPEHS 533
            TGKCIGVECYRTTF+IWGC CS GTLLALLLFART +FYS  KSPM + S
Sbjct: 537 DTGKCIGVECYRTTFMIWGCLCSLGTLLALLLFARTRSFYSTTKSPMAQGS 574

BLAST of Tan0012289 vs. ExPASy TrEMBL
Match: A0A6J1I3F0 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469568 PE=4 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 5.5e-226
Identity = 427/530 (80.57%), Postives = 461/530 (86.98%), Query Frame = 0

Query: 5   SRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLFGFFS 64
           S V QWLSL+GIIWLQSINGTNFNFPSYSSQLKHHLSISQ QLNNLAFASDAGKLF   S
Sbjct: 3   SSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVS 62

Query: 65  GLAANYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICWINTV 124
           GLAANYLPLW VLIIGS LGLVGYG+QYLFITNQ HSP+  LIFFLTVLAGNSICWINTV
Sbjct: 63  GLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWINTV 122

Query: 125 CYMVAIRNFPSNRQVAVGISTSYQGLSAKIYTNIVDSVFSKRSSKTAETFLLLNSVLPLG 184
           CY+VAIRNFPSNRQVAVGISTSYQGLSAKIYT+IV  +FSKRS KTAETFLL NSV PLG
Sbjct: 123 CYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLG 182

Query: 185 VCVLASPLARVVKGVEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSPLSGLVGI 244
           VCVL SP+AR+V+ VEEQG+L+VGFFVIFVIT+ATGVFAVVTSVGSIS MLSPLSGLVGI
Sbjct: 183 VCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVGI 242

Query: 245 LVFLFLPLIVAVVANVKETVKERKERRVYHFTVEEQKGDVEQRIDEDVERISPKNEGKLG 304
           LVFL LP++V VV +VK++V+E KERRVYH T+EE              RI P+NE KL 
Sbjct: 243 LVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED------------TRIRPENEAKLN 302

Query: 305 KESEDEEVMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRRSSSVSSLV 364
           ++  D E MEEIGA EM+KR+NFWLY GVY FGATLGLAFLNNLGQIAESR S SVSSLV
Sbjct: 303 RDGGD-EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLV 362

Query: 365 SLSSSFGFFGRLMPSLVDHFFSRRKFMKSKPGWMVALMGAMCGGFFLLSCPTNMSLCAST 424
           SLSSSFGFFGRLMPSL+DHF SRRK MKSKPGW+VALMGAMCGGFFLLS PT++ LC ST
Sbjct: 363 SLSSSFGFFGRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVST 422

Query: 425 AIIAVCTGAITSISVSTTTELFGANNFCINHNVVVANIPFGSFLFGYMAAFLYHKQAP-A 484
           A+IAVCTGAITSISVSTT+ELFGANNF INHNVVVANIP GSFLFGYMAA+LY KQA   
Sbjct: 423 AVIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGG 482

Query: 485 TGKCIGVECYRTTFLIWGCFCSFGTLLALLLFARTTTFYS-RKSPMPEHS 533
            GKCIGVECYRTTF+IWGC CSFGTLLALLLFART +FYS  KSP  + S
Sbjct: 483 AGKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGS 519

BLAST of Tan0012289 vs. ExPASy TrEMBL
Match: A0A6J1HMC6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464885 PE=4 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.6e-222
Identity = 423/531 (79.66%), Postives = 460/531 (86.63%), Query Frame = 0

Query: 5   SRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLFGFFS 64
           S V QWLSL+GIIWLQSINGTNFNFPSYSS LKHHLSISQ QLNNLAFASDAGKLF   S
Sbjct: 57  SSVFQWLSLLGIIWLQSINGTNFNFPSYSSPLKHHLSISQFQLNNLAFASDAGKLFACVS 116

Query: 65  GLAANYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICWINTV 124
           GLAANYLPLW +LIIGS LGLVGYG+QYLFITNQ HSP+  LIFFL+VLAGNSICWINTV
Sbjct: 117 GLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLSVLAGNSICWINTV 176

Query: 125 CYMVAIRNFPSNRQVAVGISTSYQGLSAKIYTNIVDSVFSKRSSKTAETFLLLNSVLPLG 184
           CY+VAIRNFPSNRQVAVGISTSYQGLSAKIYT+IV  +FSKRS KTAETFLL NSV PLG
Sbjct: 177 CYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLG 236

Query: 185 VCVLASPLARVVKGVEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSPLSGLVGI 244
           VCVL  P+AR+V+ VEE+G+L+VGFFVIFVIT+ATGVFAVVTSVGSIS MLSPLSGLVGI
Sbjct: 237 VCVLVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVGI 296

Query: 245 LVFLFLPLIVAVVANVKETVKERKERRVYHFTVEEQKGDVEQRIDEDVERISPKNEGKLG 304
           LVFL LP++V VV +VK++V+E KERRVYH T+EE              RI P+NE KL 
Sbjct: 297 LVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED------------TRIRPENEAKLN 356

Query: 305 KESEDEEVMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRRSSSVSSLV 364
           ++  D E MEEIGA EM+KR+NFWLY GVY FGATLGLAFLNNLGQIAES  S SVSSLV
Sbjct: 357 RDGGD-EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESWGSESVSSLV 416

Query: 365 SLSSSFGFFGRLMPSLVDHFFSRRKFMKSKPGWMVALMGAMCGG-FFLLSCPTNMSLCAS 424
           SLSSSFGFFGRLMPSL+DHF SRRK MKSKPGW+VALMGAMCGG FFLLS PT++ LC S
Sbjct: 417 SLSSSFGFFGRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVS 476

Query: 425 TAIIAVCTGAITSISVSTTTELFGANNFCINHNVVVANIPFGSFLFGYMAAFLYHKQAPA 484
           TA+IAVCTGAITSISVSTT+ELFGANNF INHNVVVANIPFGSFLFGYMAA+LY KQA  
Sbjct: 477 TAVIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATG 536

Query: 485 -TGKCIGVECYRTTFLIWGCFCSFGTLLALLLFARTTTFYS-RKSPMPEHS 533
            TGKCIGVECYRTTF+IWGC CS GTLLALLLFART +FYS  KSPM + S
Sbjct: 537 DTGKCIGVECYRTTFMIWGCLCSLGTLLALLLFARTRSFYSTTKSPMAQGS 574

BLAST of Tan0012289 vs. ExPASy TrEMBL
Match: A0A1S3C569 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497028 PE=4 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 8.2e-222
Identity = 425/529 (80.34%), Postives = 457/529 (86.39%), Query Frame = 0

Query: 1   MHSSSRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLF 60
           M SSS V QWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQLQLNNLAFASDAGKLF
Sbjct: 1   MPSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60

Query: 61  GFFSGLAANYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICW 120
             FSGLAANYLPLWLVL IGS LGL+GYG+QYLFITNQ HSP+ WLIFFLTVLAGNSICW
Sbjct: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICW 120

Query: 121 INTVCYMVAIRNF-PSNRQVAVGISTSYQGLSAKIYTNIVDSVFS-KRSSKTAETFLLLN 180
           INTVCYMVAI NF  SNRQVAVGISTSYQGLSAK+YT+IV S+FS K SSKTAETFLLLN
Sbjct: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180

Query: 181 SVLPLGVCVLASPLARVVKGVEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSPL 240
           SVLPLGVCVL SPLAR VK VEEQG L+VGFFVIFVIT+ATG FA +TSVGS+S MLS L
Sbjct: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240

Query: 241 SGLVGILVFLFLPLIVAVVANVKETVKERKERRVYHFTVEEQKGDVEQRIDEDVERISPK 300
           SGLVGI+VFL LPL+V V   VKE V+E KE RVYHFTVEE         + DVERI   
Sbjct: 241 SGLVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEE---------NNDVERIMRG 300

Query: 301 NEGKLGKESEDEEVMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRRSS 360
              +  + +++ E MEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESR SS
Sbjct: 301 ENERKVERTDEGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSS 360

Query: 361 SVSSLVSLSSSFGFFGRLMPSLVDHFFSRR-KFMKSKPGWMVALMGAMCGGFFLLSCPTN 420
           SVSSLVSLSSSFGFFGRL+PS++D+F SRR KFMKSKPGWMV LMGA+CGGFFLL  P++
Sbjct: 361 SVSSLVSLSSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSD 420

Query: 421 MSLCASTAIIAVCTGAITSISVSTTTELFGANNFCINHNVVVANIPFGSFLFGYMAAFLY 480
            SLC STAIIA+CTGAITSISVSTTT+LFGA NF INHNVVVANIPFGSF+FGYMAAFLY
Sbjct: 421 TSLCMSTAIIAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLY 480

Query: 481 HKQA---PATGKCIGVECYRTTFLIWGCFCSFGTLLALLLFARTTTFYS 524
           HKQA    A GKCIGVECYRTTFLIWGCF SFGT LALLLFART +FYS
Sbjct: 481 HKQAGHGVAAGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYS 520

BLAST of Tan0012289 vs. ExPASy TrEMBL
Match: A0A0A0LYD4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G533690 PE=4 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 2.7e-217
Identity = 418/527 (79.32%), Postives = 455/527 (86.34%), Query Frame = 0

Query: 3   SSSRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLFGF 62
           SSS V QWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQLQLNNLAFASDAGKLF  
Sbjct: 4   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 63

Query: 63  FSGLAANYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICWIN 122
           FSGLAANYLPLWLVL IGS LGL+GYG+QYLFITNQ HSP+ WLIFFLTVLAGNSICWIN
Sbjct: 64  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 123

Query: 123 TVCYMVAIRNF-PSNRQVAVGISTSYQGLSAKIYTNIVDSVFS-KRSSKTAETFLLLNSV 182
           TVCYMVAI NF  S+RQVAVGISTSYQGLSAK+YT+IV S+FS K SSKTAETFLLLNSV
Sbjct: 124 TVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSV 183

Query: 183 LPLGVCVLASPLARVVKGVEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSPLSG 242
           LPLGVCVL SPLAR VK VEEQG L+VGFFVIFVIT+ATG+FA +TSVGS+S MLS L G
Sbjct: 184 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGG 243

Query: 243 LVGILVFLFLPLIVAVVANVKETVKERKERRVYHFTVEEQKGDVEQRIDEDVERISPKNE 302
           LVGI+VFL LPL+V VV  VKE V+E KE +VYHFTVEE+          D ER+  +NE
Sbjct: 244 LVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEK---------NDEERMRGENE 303

Query: 303 GKLGKESEDEEVMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRRSSSV 362
            K+ + ++D E MEEIGAKEMVKRINFWLY GVYLFGATLGLAFLNNLGQIAESR SSSV
Sbjct: 304 RKV-ERTDDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSV 363

Query: 363 SSLVSLSSSFGFFGRLMPSLVDHFFSRR-KFMKSKPGWMVALMGAMCGGFFLLSCPTNMS 422
           SSLVSLSSSFGFFGRL+PS++D+F SRR KFMKSKPGWMV LMG +CGGFFLL  P++ S
Sbjct: 364 SSLVSLSSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTS 423

Query: 423 LCASTAIIAVCTGAITSISVSTTTELFGANNFCINHNVVVANIPFGSFLFGYMAAFLYHK 482
           LC STAIIA+CTGAITSISVSTTT+LFGA NF INHN+VVANIPFGSF+FGYMAAFLY K
Sbjct: 424 LCMSTAIIAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYRK 483

Query: 483 QA---PATGKCIGVECYRTTFLIWGCFCSFGTLLALLLFARTTTFYS 524
           QA      GKCIGVECYRTTFLIWG F SFGT LALLLFART +FYS
Sbjct: 484 QAGHGVDPGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYS 520

BLAST of Tan0012289 vs. ExPASy TrEMBL
Match: A0A5D3BBQ0 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00460 PE=4 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 1.0e-208
Identity = 408/529 (77.13%), Postives = 437/529 (82.61%), Query Frame = 0

Query: 1   MHSSSRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLF 60
           M SSS V QWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQLQLNNLAFASDAGKLF
Sbjct: 1   MPSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60

Query: 61  GFFSGLAANYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICW 120
             FSGLAANYLPLWLVL IGS LGL+GYG+QYLFITNQ HSP+ WLIFFLTVLAGNSICW
Sbjct: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICW 120

Query: 121 INTVCYMVAIRNF-PSNRQVAVGISTSYQGLSAKIYTNIVDSVFS-KRSSKTAETFLLLN 180
           INTVCYMVAI NF  SNRQVAVGISTSYQGLSAK+YT+IV S+FS K SSKTAETFLLLN
Sbjct: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180

Query: 181 SVLPLGVCVLASPLARVVKGVEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSPL 240
           SVLPLGVCVL SPLAR VK VEEQG L+VGFFVIFVIT+ATG FA +TSVGS+S MLS L
Sbjct: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240

Query: 241 SGLVGILVFLFLPLIVAVVANVKETVKERKERRVYHFTVEEQKGDVEQRIDEDVERISPK 300
           SGLVGI+VFL LPL+V V   VKE V+E KE RVYHFTVEE         + DVERI   
Sbjct: 241 SGLVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEE---------NNDVERIMRG 300

Query: 301 NEGKLGKESEDEEVMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRRSS 360
              +  + +++ E MEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESR SS
Sbjct: 301 ENERKVERTDEGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSS 360

Query: 361 SVSSLVSLSSSFGFFGRLMPSLVDHFFSRR-KFMKSKPGWMVALMGAMCGGFFLLSCPTN 420
           SVSSLVSLSSSFGFFGRL+PS++D+F SRR KFMKSKPGWMV LMGA+CG          
Sbjct: 361 SVSSLVSLSSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCG---------- 420

Query: 421 MSLCASTAIIAVCTGAITSISVSTTTELFGANNFCINHNVVVANIPFGSFLFGYMAAFLY 480
                         GAITSISVSTTT+LFGA NF INHNVVVANIPFGSF+FGYMAAFLY
Sbjct: 421 --------------GAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLY 480

Query: 481 HKQA---PATGKCIGVECYRTTFLIWGCFCSFGTLLALLLFARTTTFYS 524
           HKQA    A GKCIGVECYRTTFLIWGCF SFGT LALLLFART +FYS
Sbjct: 481 HKQAGHGVAAGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYS 496

BLAST of Tan0012289 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 438.0 bits (1125), Expect = 1.1e-122
Identity = 258/530 (48.68%), Postives = 343/530 (64.72%), Query Frame = 0

Query: 4   SSRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLFGFF 63
           S ++L WLSLV I+WLQS NGTN +FP+YSSQLK  L ISQ +LN L+FASDAGK+ GF 
Sbjct: 2   SRKILPWLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGFI 61

Query: 64  SGLAANYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICWINT 123
           SG+AA YLPL LVL+ G  LG  GYGLQYL I  ++                  ICWINT
Sbjct: 62  SGIAAVYLPLPLVLLAGGSLGFAGYGLQYLSIIKKI------------------ICWINT 121

Query: 124 VCYMVAIRNFPSNRQVAVGISTSYQGLSAKIYTNIVDSVFSKRSSKTAETFLLLNSVLPL 183
            CY+VAI +FP NRQVAVGI+ SYQGLS KIYT++V S F     + A  +LLLNS++PL
Sbjct: 122 ACYIVAINSFPVNRQVAVGITASYQGLSGKIYTDMVHSFFHTSQREEASGYLLLNSLVPL 181

Query: 184 GVCVLASPLARVVKG---VEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSPLSG 243
             C++ +P+     G   +     +KVGF V+FV+T+ATG++AV TS+ S+  +L     
Sbjct: 182 VACLVTAPMLMRHGGDKTMSYSKDVKVGFIVLFVLTIATGIYAVATSLVSVPAVLV---- 241

Query: 244 LVGILVFLFLPLIVAVVANVKETVKERKERRVYHFTVEEQKGDVEQRIDE--DVERISPK 303
           LVGI +FL  PL + +    KE +  RK ++  H        D+E  +D+   VE    K
Sbjct: 242 LVGIALFLLAPLAIPIGVGFKELMSSRKTQQKVH--------DLEAPVDKFYFVEEDHTK 301

Query: 304 NEGKLGKESEDEEVMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRRSS 363
            E +   E     V EE+   ++ K+++FW+YFG+YLFG T+GL F NNLGQIAESR S+
Sbjct: 302 EEEEF--EKAIIGVKEEVEWTQLWKKLDFWIYFGLYLFGPTVGLVFTNNLGQIAESRGST 361

Query: 364 SVSSLVSLSSSFGFFGRLMPSLVDHFFSRRKFMKSKPGWMVALMGAMCGGFFLLSCPTNM 423
           + SSLV+LSSSFGFFGRL+PSL+D+FFSR K+M S P  M   + AM   F LL   +++
Sbjct: 362 ATSSLVALSSSFGFFGRLLPSLLDYFFSRNKYMPSSPVSMAGSLVAMVASFLLLLIDSDI 421

Query: 424 SLCASTAIIAVCTGAITSISVSTTTELFGANNFCINHNVVVANIPFGSFLFGYMAAFLYH 483
           +L   TA+I + +GA+TS+SV+ T ELFG  +F +NHN+VV +IP GSF FG +AA +Y 
Sbjct: 422 ALYIGTAMIGIFSGALTSLSVTMTAELFGTKHFGVNHNIVVGSIPLGSFSFGLLAAKIYR 481

Query: 484 KQAPA---TGKCIGVECYRTTFLIWGCFCSFGTLLALLLFARTTTFYSRK 526
             A      GKC G+ C++TT + WG  CS   LLA +L+ R   FYS+K
Sbjct: 482 DGAALYGDDGKCFGMHCFQTTLVFWGMLCSIAALLAAVLYIRNRKFYSQK 499

BLAST of Tan0012289 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 284.6 bits (727), Expect = 1.6e-76
Identity = 195/539 (36.18%), Postives = 299/539 (55.47%), Query Frame = 0

Query: 9   QWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLFGFFSGLAA 68
           +W  LV  IW+Q+  GTNF+F +YSS LK  L ISQ++LN LA ASD GK FG+ SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 69  NYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICWINTVCYMV 128
            Y PL +VL   + +G VGYG+Q+L ITN +  P + L+F   +LAG SICW NT C+++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYS-LVFLCCLLAGLSICWFNTACFIL 162

Query: 129 AIRNFPSNRQVAVGISTSYQGLSAKIYTNIVDSVFSKRSSKTAETFLLLNSVLPLGVC-- 188
            IR+FP+NR +A+ ++ S+ G+SA +Y+      F+  +  ++  +LLLNS++PL V   
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYS----LAFNAINPSSSNLYLLLNSLVPLVVSFA 222

Query: 189 ----VLASPLARVVKGVEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSPLSGLV 248
               VL  P        + +      F ++ V+ V T    +++S  + S  L+     +
Sbjct: 223 ALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLN----FI 282

Query: 249 GILVFLFLPLIVAVVANVKETVKERKERRVYHFTVEEQKGDVEQRIDEDVERISPKNEGK 308
           G +V L  PL   ++   ++        R+ H    E  G V   IDE ++        K
Sbjct: 283 GAVVLLVFPLCAPLLVYARDYFLPVINARLNH----ESSGYVMLNIDE-LKNQKTSVSSK 342

Query: 309 LG-------KESEDEEVMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESR 368
            G       KE     + +E   + ++ R+ FWLY+  Y  G T+GL + NNLGQIA+S 
Sbjct: 343 TGYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSL 402

Query: 369 RSSSVSSLVSLSSSFGFFGRLMPSLVDHFFSRRKFMKSKPGWMVALMGAMCGGFFLLSCP 428
             +S ++LV++ SSF FFGRL+ +  D  F  ++F  ++ GW    +      FFLL+  
Sbjct: 403 GQNS-TTLVTIYSSFSFFGRLLSAAPD--FMHKRFRLTRTGWFAIALLPTPIAFFLLAVS 462

Query: 429 TNM--SLCASTAIIAVCTGAITSISVSTTTELFGANNFCINHNVVVANIPFGSFLFGYMA 488
           ++   +L  +TA+I + +G I + +VS T++LFG N+  +NHN+++ NIP GS L+GY+A
Sbjct: 463 SSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIA 522

Query: 489 AFLYHKQA-----PATGK---CIGVECYRTTFLIWGCFCSFGTLLALLLFARTTTFYSR 525
           A +Y   A     P       CIG +CY  TF+ WGC    G + +L L+ RT   Y R
Sbjct: 523 ASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHR 564

BLAST of Tan0012289 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 283.5 bits (724), Expect = 3.5e-76
Identity = 209/562 (37.19%), Postives = 310/562 (55.16%), Query Frame = 0

Query: 9   QWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLFGFFSGLAA 68
           +W+ LV  IW+Q+  GTNF+F +YSS LK  L ISQ+QLN LA ASD GK+FG+ SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 69  NYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICWINTVCYMV 128
            Y PLW VL   + +G VGYG+Q+L ITN +  P   L+F   +LAG SICW NTVC+++
Sbjct: 67  LYFPLWTVLFAAAIMGFVGYGVQWLVITNVISLP-YILVFLCCLLAGLSICWFNTVCFVL 126

Query: 129 AIRNFPSNRQVAVGISTSYQGLSAKIYTNIVDSVFSKRSSKTAETFLLLNSVLPLGVCVL 188
            IRNFP+NR +A+ ++ S+ G+SA +YT   +++    +  + E +LLLN+++PL V   
Sbjct: 127 CIRNFPANRSLALSLTVSFNGVSAALYTLAYNAI----NPVSTELYLLLNALVPLFVSFA 186

Query: 189 A-------SPLARVVKGVEEQGSLKVGFFVIFVITVATGVFAVV-TSVGSISTMLSPLSG 248
           A        PL  +      + SL   F ++ ++ V  GV+ ++  S  S  T    L G
Sbjct: 187 ALIPILRQPPLEPLPPDGVRRDSLM--FLLLNILAVLNGVYLLLFRSKTSDVTSARLLFG 246

Query: 249 LVGILVFLFLPLIVAVVANVKETVKE------RKERRVYHFT----VEEQKGDVE----- 308
             G ++ L LPL +  +   +           R E   +       +E  KG V      
Sbjct: 247 --GSILLLILPLCLPGLVYARNWYLHNIHSSFRLEGSGFILVDVDELEMHKGMVTREASL 306

Query: 309 ---QRIDEDVERISPKNEGKLGKESED-------------EEVMEEIGAKEMVKRINFWL 368
              Q +++DV R     + K   E +D               + EE     ++ R +FWL
Sbjct: 307 EGYQLLNDDVVRAVNTPDQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRSDFWL 366

Query: 369 YFGVYLFGATLGLAFLNNLGQIAES-RRSSSVSSLVSLSSSFGFFGRLMPSLVDHFFSRR 428
           Y+  Y  G T+GL + NNLGQIA+S  +SS  ++LV+L SSF FFGRL+ +  D+   R 
Sbjct: 367 YYIAYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPDYI--RA 426

Query: 429 KFMKSKPGWM-VALMGAMCGGFFLLSCPTNMSLCASTAIIAVCTGAITSISVSTTTELFG 488
           K   ++ GW+ VAL+      F L S  +  +L A TA+I + +G I + +VS T+ELFG
Sbjct: 427 KVYFARTGWLAVALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSELFG 486

Query: 489 ANNFCINHNVVVANIPFGSFLFGYMAAFLYHKQAPATGK-----CIGVECYRTTFLIWGC 525
            N+  +NHN+++ NIP GS ++G++AA +Y   + A  K     C+G +CY  TF+ WGC
Sbjct: 487 PNSVGVNHNILITNIPIGSLVYGFLAALVYESHSVAGSKTESVICMGRDCYLQTFMWWGC 546

BLAST of Tan0012289 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 1.3e-73
Identity = 202/566 (35.69%), Postives = 314/566 (55.48%), Query Frame = 0

Query: 9   QWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLFGFFSGLAA 68
           +W+ LV  IW+Q+  GTNF+F +YSS LK  L ISQ+QLN LA ASD GK+FG+ SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 69  NYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICWINTVCYMV 128
            Y PLW VL   +F+G VGYG+Q+L IT+ +  P   ++F   +LAG SICW NTVC+++
Sbjct: 67  MYFPLWTVLFAAAFMGFVGYGVQWLVITHFISLP-YIMVFLCCLLAGLSICWFNTVCFVL 126

Query: 129 AIRNFPSNRQVAVGISTSYQGLSAKIYTNIVDSVFSKRSSKTAETFLLLNSVLPLGVCVL 188
            I NFP+NR +A+ ++ S+ G+SA +YT   +++    +  + E +LLLN+++PL V   
Sbjct: 127 CISNFPANRSLALSLTVSFNGVSAALYTLAYNAI----NPTSPELYLLLNALIPLIVSFT 186

Query: 189 A-SPLAR-------VVKGVEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSP--- 248
           A  P+ R          GV     +   F ++ ++    GV+ ++   GS S+ L+    
Sbjct: 187 AIIPILRQPPFEPLPPDGVRRDSLM---FLLLNILAALNGVYLLL--FGSNSSDLTSARL 246

Query: 249 -LSGLVGILVF-LFLPLIVAVVANVKETVK-------------ERKERRVYH-FTVEEQK 308
              G + +LVF L +P +V        T+              +  E  ++      E  
Sbjct: 247 LFGGAILLLVFPLCIPGLVIARNWYNRTIHTSFRLEGSGFILVDPDELELHKGMLAHEAN 306

Query: 309 GDVEQRIDEDVERISPKNEGKLGKESEDE---------EVMEEIGAKE----MVKRINFW 368
            +  Q + +DV + +P     + +E  DE         + +E +G +     ++ R +FW
Sbjct: 307 REGYQLLSDDVVQ-NPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRSDFW 366

Query: 369 LYFGVYLFGATLGLAFLNNLGQIAES-RRSSSVSSLVSLSSSFGFFGRLMPSLVDHFFSR 428
           LY+  Y  G T+GL + NNLGQIA+S  +SS+ ++LV+L S+F FFGRL+ +  D+   R
Sbjct: 367 LYYITYFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPDYI--R 426

Query: 429 RKFMKSKPGWM-VALMGAMCGGFFLLSCPTNMSLCASTAIIAVCTGAITSISVSTTTELF 488
            K   ++ GW+ +AL+      F L S  T  +L A TA++ + +G I + +VS T+ELF
Sbjct: 427 AKVYFARTGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSITSELF 486

Query: 489 GANNFCINHNVVVANIPFGSFLFGYMAAFLYHKQAPATGK--------CIGVECYRTTFL 525
           G N+  +NHN+++ NIP GS ++G++AA +Y        K        C+G +CY  TF+
Sbjct: 487 GPNSVGVNHNILITNIPIGSLIYGFLAALVYDSHGFTGTKSMTSESVVCMGRDCYYLTFV 546

BLAST of Tan0012289 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 8.4e-70
Identity = 186/536 (34.70%), Postives = 288/536 (53.73%), Query Frame = 0

Query: 9   QWLSLVGIIWLQSINGTNFNFPSYSSQLKHHLSISQLQLNNLAFASDAGKLFGFFSGLAA 68
           +W  LV  IW+Q+  GTNF+F +YSS +K  + +SQ +LN +A ASD GK  G+ SG A 
Sbjct: 23  RWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSRLNYMAVASDLGKALGWSSGFAI 82

Query: 69  NYLPLWLVLIIGSFLGLVGYGLQYLFITNQLHSPANWLIFFLTVLAGNSICWINTVCYMV 128
            Y P+  VL   + +GLVGYG+Q+L I + +  P + L+     LAG SICW NTV +++
Sbjct: 83  AYFPVSGVLFAAAAMGLVGYGVQWLSIADVIDLPYS-LVLVCCSLAGLSICWFNTVSFIL 142

Query: 129 AIRNFPSNRQVAVGISTSYQGLSAKIYTNIVDSVFSKRSSKTAETFLLLNSVLPLGVCVL 188
            IR+F +N  +A+ +  S+ G+SA +YT +     S +SS +++ +LLLNS++PL V VL
Sbjct: 143 CIRHFKANHSLALSLVVSFNGISAALYT-LGHEAISGKSSASSDIYLLLNSLIPLIVSVL 202

Query: 189 A------SPLARVVKGVEEQGSLKVGFFVIFVITVATGVFAVVTSVGSISTMLSPLSGLV 248
           A      +P +            ++ F V  V+ + T  + ++ S G+     SP    +
Sbjct: 203 ALWPVLTNPNSSETDTTRTHDETRI-FVVFNVLALVTCFYLLLPSSGTYLAS-SPRWHFL 262

Query: 249 GILVFLFLPLIVAVVANVKETVKERKERRVYHFTVEEQKGDVEQRIDEDVERISPKNEGK 308
           G +  L  PL V  +  +   ++       +H       G     I+E         +  
Sbjct: 263 GAIFLLLFPLCVPFLDYIHRALES-----CFH---HHSSGYAAVNIEEPKILKIKSQKSN 322

Query: 309 LGKESEDEEVMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAES--RRSSSV 368
             +ES+   + +E     +V+++ FWLY+  Y  G T+GL + NNLGQIA+S  + SS+ 
Sbjct: 323 AEEESDQVRLGDEHSLGMLVRKLEFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGQSSSNA 382

Query: 369 SSLVSLSSSFGFFGRLMPSLVDHFFSRRKFMKSKPGWMVALMGAMCGGFFLL--SCPTNM 428
            SLV+L S+F F GRL+ S  D    + K++ ++ GW    +      FF+L  S  TN 
Sbjct: 383 KSLVTLFSAFSFLGRLLSSAPDFTRKKLKYL-TRTGWFTISLLPTPLAFFILAYSPKTNQ 442

Query: 429 S--LCASTAIIAVCTGAITSISVSTTTELFGANNFCINHNVVVANIPFGSFLFGYMAAFL 488
           +  L  +TA+I + +G + + +VS T+ELFG N+  +N N+++ NIP GS  +GYMA  +
Sbjct: 443 TALLEVATALIGLSSGFVFAAAVSITSELFGRNSVGVNQNILITNIPIGSLFYGYMAGSV 502

Query: 489 YHKQAPATGK--------CIGVECYRTTFLIWGCFCSFGTLLALLLFARTTTFYSR 525
           Y   A    K        C+G +CY  TFL WGC    G + +L LF RT   Y R
Sbjct: 503 YDTNASLGRKSVVADSVVCVGRKCYFVTFLFWGCLSVLGFVCSLFLFIRTRPVYHR 545