Tan0012214 (gene) Snake gourd v1

BLAST of Tan0012214 vs. ExPASy Swiss-Prot
Match: Q5XLY0 (Putative anthocyanidin reductase OS=Ginkgo biloba OX=3311 PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 4.0e-42
Identity = 108/333 (32.43%), Postives = 184/333 (55.26%), Query Frame = 0

Query: 9   SSTVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFKAD 68
           ++TVCVTG +GF+ S L+KRLL  G+ VHAT+R+  +++KV  L +LP A   L LF+A+
Sbjct: 12  TTTVCVTGAAGFMASWLVKRLLEKGYIVHATVRDPENKAKVSHLLNLPGATDRLKLFRAE 71

Query: 69  IYKPDDFEDAIRGCQIVFHVATPLQHTHGSQYKNRTEASIATAKNIAKLCVESGTVRRLI 128
           + +   F+ A+ GC  VFHVATP +        +  + +I    N+ K C +  +++R++
Sbjct: 72  LCEDGSFDAAVAGCNGVFHVATPTEFMPKDPENDLIKPAIEGTLNVLKSCTKVDSIKRVV 131

Query: 129 YTASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPFSNTSFEEYIDSKTLTEKVLLEFGE 188
            T+S  + S   ++ S   +++DE+CWT ++         +  Y  SKTL E+  L++ E
Sbjct: 132 VTSSAATVS--INNSSEQNQYIDESCWTDVNFLTSQKPPGW-AYPVSKTLAEQAALKYAE 191

Query: 189 SEESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRFIEELDGKVPLV 248
                 L+VV++   L+ G ++  +   +  +  S  T       AL+ ++ + G + LV
Sbjct: 192 EH---SLDVVTVIPVLVVGPAVTPTVPSSVELALSLITGDEFKMGALKGMQFVSGSISLV 251

Query: 249 HIDDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLHQKHGNSDEVP-TRN 308
           HIDD+C A IF ME+ S  GR++C         +A +    +PQ ++     D+VP T  
Sbjct: 252 HIDDVCSAQIFLMEKPSAQGRYICFPVNTGIPQLAEFLSKRYPQ-YKVPTKFDDVPATPK 311

Query: 309 IKMNSKKLIERGFIYKYDDDMILEDAYECYKKK 341
           + ++S+KL++ GF +KY  + I + A E  K K
Sbjct: 312 LTISSQKLLDCGFSFKYGIEDIYDQAIEYMKTK 337

BLAST of Tan0012214 vs. ExPASy Swiss-Prot
Match: Q7PCC4 (Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN=ANR PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 2.9e-40
Identity = 102/335 (30.45%), Postives = 175/335 (52.24%), Query Frame = 0

Query: 11  TVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFKADIY 70
           T CV GG+GFV S L+K LL  G+ V+ T+R+ +++ KV  L  L     +L +F+AD+ 
Sbjct: 11  TACVVGGTGFVASLLVKLLLQKGYAVNTTVRDPDNQKKVSHLLELQELG-DLKIFRADLT 70

Query: 71  KPDDFEDAIRGCQIVFHVATPLQHTHGSQYKNRTEASIATAKNIAKLCVESGTVRRLIYT 130
               FE  I GC  VFHVATP+         +  + +I    N+ K C  + +V+R+I T
Sbjct: 71  DELSFEAPIAGCDFVFHVATPVHFASEDPENDMIKPAIQGVVNVMKACTRAKSVKRVILT 130

Query: 131 ASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPFSNTSFEEYIDSKTLTEKVLLEFGESE 190
           +S  + +  + DG+G    +DE  WT +         ++  Y  SKTL EK   +F    
Sbjct: 131 SSAAAVTINQLDGTGL--VVDEKNWTDIEFLTSAKPPTW-GYPASKTLAEKAAWKFA--- 190

Query: 191 ESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRFIEELDGKVPLVHI 250
           E   ++++++   L++G SL S    +  +  S  T        ++ ++ L G V + H+
Sbjct: 191 EENNIDLITVIPTLMAGSSLTSDVPSSIGLAMSLITGNEFLINGMKGMQMLSGSVSIAHV 250

Query: 251 DDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLHQKHGNSDEVPTRNIKM 310
           +D+C+AHIF  E+ S  GR++C ++  S  ++A +    +PQ        D  P   + +
Sbjct: 251 EDVCQAHIFVAEKESASGRYICCAANTSVPELAKFLSKRYPQYKVPTDFGDFPPKSKLII 310

Query: 311 NSKKLIERGFIYKYDDDMILEDAYECYKKKEMFVN 346
           +S+KL++ GF +KY  + I +++ E +K K +  N
Sbjct: 311 SSEKLVKEGFSFKYGIEEIYDESVEYFKAKGLLQN 338

BLAST of Tan0012214 vs. ExPASy Swiss-Prot
Match: Q5FB34 (Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN=ANR PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 4.9e-40
Identity = 102/335 (30.45%), Postives = 174/335 (51.94%), Query Frame = 0

Query: 11  TVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFKADIY 70
           T CV GG+GFV S L+K LL  G+ V+ T+R+ +++ KV  L  L     +L +F+AD+ 
Sbjct: 11  TACVVGGTGFVASLLVKLLLQKGYAVNTTVRDPDNQKKVSHLLELQELG-DLKIFRADLT 70

Query: 71  KPDDFEDAIRGCQIVFHVATPLQHTHGSQYKNRTEASIATAKNIAKLCVESGTVRRLIYT 130
               FE  I GC  VFHVATP+         +  + +I    N+ K C  + +V+R+I T
Sbjct: 71  DELSFEAPIAGCDFVFHVATPVHFASEDPENDMIKPAIQGVVNVMKACTRAKSVKRVILT 130

Query: 131 ASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPFSNTSFEEYIDSKTLTEKVLLEFGESE 190
           +S  + +  + DG+G    +DE  WT +         ++  Y  SKTL EK   +F    
Sbjct: 131 SSAAAVTINQLDGTGL--VVDEKNWTDIEFLTSAKPPTW-GYPASKTLAEKAAWKFA--- 190

Query: 191 ESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRFIEELDGKVPLVHI 250
           E   ++++++   L++G SL S    +  +  S  T        ++ ++ L G V + H+
Sbjct: 191 EENNIDLITVIPTLMAGSSLTSDVPSSIGLAMSLITGNEFLINGMKGMQMLSGSVSIAHV 250

Query: 251 DDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLHQKHGNSDEVPTRNIKM 310
           +D+C AHIF  E+ S  GR++C ++  S  ++A +    +PQ        D  P   + +
Sbjct: 251 EDVCRAHIFVAEKESASGRYICCAANTSVPELAKFLSKRYPQYKVPTDFGDFPPKSKLII 310

Query: 311 NSKKLIERGFIYKYDDDMILEDAYECYKKKEMFVN 346
           +S+KL++ GF +KY  + I +++ E +K K +  N
Sbjct: 311 SSEKLVKEGFSFKYGIEEIYDESVEYFKAKGLLQN 338

BLAST of Tan0012214 vs. ExPASy Swiss-Prot
Match: P51103 (Dihydroflavonol 4-reductase OS=Callistephus chinensis OX=13379 GN=F PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 1.4e-39
Identity = 109/333 (32.73%), Postives = 171/333 (51.35%), Query Frame = 0

Query: 11  TVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFKADIY 70
           TVCVTG +GF+GS L+ RLL  G+ V AT+RN  D  KV  L  LP A TNL L+KAD+ 
Sbjct: 8   TVCVTGAAGFIGSWLVMRLLERGYIVRATVRNPGDMKKVKHLLELPKAETNLTLWKADLT 67

Query: 71  KPDDFEDAIRGCQIVFHVATPLQHTHGSQYKNRTEASIATAKNIAKLCVESGTVRRLIYT 130
           +   F++AI GC  VFHVATP+            + +I    +I + C ++ TV++L+YT
Sbjct: 68  QEGSFDEAIEGCHGVFHVATPMDFESKDPENEIIKPTIEGILSIIRSCAKAKTVKKLVYT 127

Query: 131 ASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPFSNTSFEEYIDSKTLTEKVLLEFGESE 190
           +S  + +  +          DE+ W+ L   Y    T++  Y  SKTL EK  +   E+ 
Sbjct: 128 SSAGTVNVQETQ----LPVYDESHWSDLDFIYSKKMTAW-MYFVSKTLAEKAAM---EAA 187

Query: 191 ESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRFIEELDGKVPLVHI 250
           +   ++ VS+   L+ G  ++ +   + I   S        +  ++       +   VH+
Sbjct: 188 KENNIDFVSIIPPLVVGPFINPTFPPSLITALSLINGAESHYSIIK-------QGQYVHL 247

Query: 251 DDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLH---QKHGNSDEVPTRN 310
           DD+CE HIF  E     GR++C+    +   +A       P+ H   Q  G  +E+PT  
Sbjct: 248 DDLCECHIFLYENPEAKGRYICSKQDATIHQLARMIKQKWPEYHVPTQFAGIDEELPT-- 307

Query: 311 IKMNSKKLIERGFIYKYDDDMILEDAYECYKKK 341
           +  +SKKLI+ GF +KYD + + + A +  K+K
Sbjct: 308 VSFSSKKLIDMGFKFKYDLEDMFKGAIDSCKEK 323

BLAST of Tan0012214 vs. ExPASy Swiss-Prot
Match: P14720 (Dihydroflavonol 4-reductase OS=Petunia hybrida OX=4102 GN=DFRA PE=2 SV=2)

HSP 1 Score: 166.0 bits (419), Expect = 1.9e-39
Identity = 106/335 (31.64%), Postives = 174/335 (51.94%), Query Frame = 0

Query: 11  TVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFKADIY 70
           TVCVTG +GF+GS L+ RLL  G+ VHAT+R+  ++ KV  L  LP A+TNL L+KAD+ 
Sbjct: 17  TVCVTGAAGFIGSWLVMRLLERGYNVHATVRDPENKKKVKHLLELPKADTNLTLWKADLT 76

Query: 71  KPDDFEDAIRGCQIVFHVATPLQHTHGSQYKNRTEASIATAKNIAKLCVESGTVRRLIYT 130
               F++AI+GCQ VFHVATP+            + ++    +I + C ++ TV+RL++T
Sbjct: 77  VEGSFDEAIQGCQGVFHVATPMDFESKDPENEVIKPTVRGMLSIIESCAKANTVKRLVFT 136

Query: 131 ASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPFSNTSFEEYIDSKTLTEKVLLEFGESE 190
           +S  +    +      K F D+T W+ L   Y    T +  Y  SK L EK  +E  + +
Sbjct: 137 SSAGTLDVQEQQ----KLFYDQTSWSDLDFIYAKKMTGW-MYFASKILAEKAAMEEAKKK 196

Query: 191 ESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRFIEELDGKVPLVHI 250
               ++ +S+   L+ G  +  +   + I   S  T     +  ++       +   VH+
Sbjct: 197 ---NIDFISIIPPLVVGPFITPTFPPSLITALSLITGNEAHYCIIK-------QGQYVHL 256

Query: 251 DDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLH---QKHGNSDEVPTRN 310
           DD+CEAHIF  E     GRF+C+S      D+A       P+ +   +  G   ++P   
Sbjct: 257 DDLCEAHIFLYEHPKADGRFICSSHHAIIYDVAKMVREKWPEYYVPTEFKGIDKDLPV-- 316

Query: 311 IKMNSKKLIERGFIYKYDDDMILEDAYECYKKKEM 343
           +  +SKKL + GF +KY  + + + A +  ++K++
Sbjct: 317 VSFSSKKLTDMGFQFKYTLEDMYKGAIDTCRQKQL 334

BLAST of Tan0012214 vs. NCBI nr
Match: QCD77544.1 (anthocyanidin reductase [Vigna unguiculata])

HSP 1 Score: 691.8 bits (1784), Expect = 7.5e-195
Identity = 374/711 (52.60%), Postives = 483/711 (67.93%), Query Frame = 0

Query: 10  STVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFKADI 69
           S VCVTGGSG++GS LI +LL  G+TVHATLR+L +ESKVGLLKSLP+A   LVLF+ADI
Sbjct: 5   SKVCVTGGSGYIGSWLINKLLAKGYTVHATLRDLKNESKVGLLKSLPHAEGKLVLFEADI 64

Query: 70  YKPDDFEDAIRGCQIVFHVATPLQHTHG-SQYKNRTEASIATAKNIAKLCVESGTVRRLI 129
           Y P  F+ AI+GC+ VFHVATPL H  G SQYK+ TEA++A  K+IA  CV SGTV+RLI
Sbjct: 65  YNPTQFDPAIQGCEYVFHVATPLAHEPGSSQYKDTTEAAVAGTKSIAMSCVRSGTVKRLI 124

Query: 130 YTASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPFSNTSFEEYIDSKTLTEKVLLEFGE 189
           YTASVVS +P+K+DG  FK+ MDETCWTPLH S    +  +++YI SKT +EK +L +G 
Sbjct: 125 YTASVVSAAPLKEDGRDFKDVMDETCWTPLHDSLYLHDPFYKDYIFSKTTSEKHVLSYGN 184

Query: 190 SEESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRFIEELDGKVPLV 249
            E  G LEVV+L CGL+ GD+L S+ +  T V  +Q      A+ +L+F+E L GK+PLV
Sbjct: 185 DENGGGLEVVTLPCGLVGGDTLLSTPSTRT-VCIAQIVQNERAYKSLKFLEGLLGKIPLV 244

Query: 250 HIDDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLHQKHGNSDEVPTRNI 309
           HIDD+C+AHIFCME  SI GRFLCASS++S  ++AN+Y L++P+   K  N D    ++I
Sbjct: 245 HIDDVCDAHIFCMETPSITGRFLCASSYISLQEMANHYALYYPEFTVKQENEDG-ERKDI 304

Query: 310 KMNSKKLIERGFIYKYDDDMILEDAYECYKKK-EMFVNSPVLTPI--------------- 369
           K +S KL E+GF+YKYD  MIL+D  +C ++  E++ N     PI               
Sbjct: 305 KWSSTKLCEKGFVYKYDAKMILDDCIKCARRMGELYCNCLWCIPICVKIRKMGEGSKVCV 364

Query: 370 SGGSGFVASSLIKTLLQNGHTVHATLRNFNDESKVELLKSLPNATTNLVLFQADIYEPHQ 429
           +GGSG++ S LI  LL  G+TVHATLR+  +ESKV LLKSLP+A   LVLF+ADIY P Q
Sbjct: 365 TGGSGYIGSWLINKLLAKGYTVHATLRDLKNESKVGLLKSLPHAEGKLVLFEADIYNPTQ 424

Query: 430 FQAAIQGSHVVFHVAT-LFHHTHESQYKNTTEASVAAAKKIAKLCVESRTVRRLIYTGSV 489
           F  AIQG   VFHVAT L H    SQYK+TTEA+VA  K IA  CV S TV+RLIYT SV
Sbjct: 425 FDPAIQGCEYVFHVATPLAHEPGSSQYKDTTEAAVAGTKSIAMSCVRSGTVKRLIYTASV 484

Query: 490 VSASPMKEDGSGFKEFMDETCWTPL--NLSYPFSN-PIFKGYVESKTLTEKELLRFGESE 549
           V+ASP+KEDGS FK+ MDETCWTPL  +L Y F + P++K Y  SKTL+EK LL +G  E
Sbjct: 485 VAASPLKEDGSDFKDVMDETCWTPLHDSLEYLFLDVPLYKDYTYSKTLSEKHLLSYGNDE 544

Query: 550 ESGG-LEVVSLACGVIVGDSLHSYAADSTIVTFSQFTDQTSAFYALRFMEELDGKVPLVH 609
             GG LEVV+LACG++ GD L S A  S ++  +Q       +  L+F+E L GK+PL H
Sbjct: 545 NGGGKLEVVTLACGLVGGDCLVSSALSSRLICIAQIVQNEMTYKTLKFLEGLLGKIPLAH 604

Query: 610 MNDVIEAHIFCMEQSSIHGRFFCATSFLSSSDIANYYRLHHPQLHQKHGSVLDSHSLTIV 669
           ++DV EAHIFCME +S+ GRF CA+S++S  ++AN+Y  +HP+ + K             
Sbjct: 605 IDDVCEAHIFCMESTSVSGRFLCASSYISLQEMANHYARYHPEFNVKQ------------ 664

Query: 670 SFVFHLLKCLNSEDVPIRNIKLDSKKLIDKGFKYKYDGDMILEDALECYKK 699
                       ED   ++IK  S KL DKGF YKYD  MIL+D ++C ++
Sbjct: 665 ----------EDEDGQKKDIKWSSTKLCDKGFVYKYDAKMILDDCIKCARR 691

BLAST of Tan0012214 vs. NCBI nr
Match: RXI07732.1 (hypothetical protein DVH24_009763 [Malus domestica])

HSP 1 Score: 632.9 bits (1631), Expect = 4.1e-177
Identity = 349/716 (48.74%), Postives = 489/716 (68.30%), Query Frame = 0

Query: 9   SSTVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLP--NANTNLVLFK 68
           ++ VCVTGGS ++GSSLIKRLL  G+TVHATLRNL D  KVGLLK LP   A+  L LF+
Sbjct: 3   NTRVCVTGGSSYLGSSLIKRLLEKGYTVHATLRNLGDPFKVGLLKGLPTLGADNRLKLFE 62

Query: 69  ADIYKPDDFEDAIRGCQIVFHVATPLQH-THGSQYKNRTEASIATAKNIAKLCVESGTVR 128
           ADIY PD+FE AI+GC  +FH+ATPLQH    +Q+K+ +EA+++  K+I + C++SGTV+
Sbjct: 63  ADIYNPDEFETAIQGCNYIFHLATPLQHEPKDTQFKDTSEAAVSGIKSIVRSCIKSGTVK 122

Query: 129 RLIYTASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPFSNTSFEEYIDSKTLTEKVLLE 188
           RLIYTASVV+ SP+KD G+GFK+FMDE+CWTPL+LS+ F+N    +Y  SK+L+EK +L+
Sbjct: 123 RLIYTASVVAASPIKDSGNGFKQFMDESCWTPLNLSFAFANKVLIDYTHSKSLSEKEVLK 182

Query: 189 FGESEESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRFIEELDGKV 248
                 SG+LEVVS+ACGL+ GD+L ++   +  V  SQ T  +  +  LRF+EEL GKV
Sbjct: 183 ----HTSGDLEVVSIACGLVGGDTLLTTMPESMGVLISQITKDSRRYQTLRFLEELLGKV 242

Query: 249 PLVHIDDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLHQKHGNSDEVPT 308
           P+VHI+D+CEAHI CME+ SI+GRFLC S++LSS++IA+++  ++P L        E   
Sbjct: 243 PIVHIEDVCEAHILCMEKPSINGRFLCVSAYLSSAEIASHWEKNYPSLKIAE-EFVEDSG 302

Query: 309 RNIKMNSKKLIERGFIYKYDDDMILEDAYECYKKKEMFVNSP-VLTP---ISGGSGFVAS 368
           R+I   S KL + GF YK+D  +IL+D  +  +    F +S   + P   ++GGS ++ S
Sbjct: 303 RDIGWGSTKLKKIGFEYKFDAKVILDDTLKWAQTMGEFASSQNTVVPKVCVTGGSSYLGS 362

Query: 369 SLIKTLLQNGHTVHATLRNFNDESKVELLKSL--PNATTNLVLFQADIYEPHQFQAAIQG 428
           SLIK LL+ G+TVHATLRN  D  KV LLK L  P A + L LF+ADIY P +F+ AIQG
Sbjct: 363 SLIKGLLEKGYTVHATLRNLGDPFKVGLLKGLPTPGADSRLKLFEADIYNPDEFETAIQG 422

Query: 429 SHVVFHVAT-LFHHTHESQYKNTTEASVAAAKKIAKLCVESRTVRRLIYTGSVVSASPMK 488
            + +FH+AT L H   ++Q+K+T+EA+V+  K I + C++S TV+RLIYT SVV+ASP+K
Sbjct: 423 CNYIFHLATPLQHEPKDTQFKDTSEAAVSGIKSIVRSCIKSGTVKRLIYTASVVAASPIK 482

Query: 489 EDGSGFKEFMDETCWTPLNLSYPFSNPIFKGYVESKTLTEKELLRFGESEESGGLEVVSL 548
           + G+GFK+FMDE+CWTPLNLS+ F+N +   Y  SK+L+EKE+L+      SG LEVVS+
Sbjct: 483 DSGNGFKQFMDESCWTPLNLSFAFANKVLIDYTHSKSLSEKEVLK----HTSGDLEVVSI 542

Query: 549 ACGVIVGDSLHSYAADSTIVTFSQFTDQTSAFYALRFMEELDGKVPLVHMNDVIEAHIFC 608
           ACG++ GD+L +   +S  V  SQ T  +  +  LRF+EEL GKVP+VH+ DV EAHI C
Sbjct: 543 ACGLVGGDTLLTAMPESMGVLISQITKDSRRYQTLRFLEELLGKVPIVHIEDVCEAHILC 602

Query: 609 MEQSSIHGRFFCATSFLSSSDIANYYRLHHPQLHQKHGSVLDSHSLTIVSFVFHLLKCLN 668
           ME+ SI+GRF C +++LSS++IA+++  ++P L      V DS                 
Sbjct: 603 MEKPSINGRFLCVSAYLSSAEIASHWEKNYPSLRITEEFVEDSG---------------- 662

Query: 669 SEDVPIRNIKLDSKKLIDKGFKYKYDGDMILEDALECYKKKEMVNNSIKLSPTSEK 715
                 R+I   S KL   GF+Y +D  +IL+D L+  +K   V   I L P++++
Sbjct: 663 ------RDIGWGSTKLKKIGFEYNFDAKVILDDTLKWAQK--WVPAKILLYPSNDQ 685

BLAST of Tan0012214 vs. NCBI nr
Match: OMO64318.1 (NAD-dependent epimerase/dehydratase [Corchorus capsularis])

HSP 1 Score: 617.5 bits (1591), Expect = 1.8e-172
Identity = 336/693 (48.48%), Postives = 457/693 (65.95%), Query Frame = 0

Query: 12  VCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFKADIYK 71
           VCVTGG+G++GSSL+K LL  G+TVHATLRNL D SKV  LKS P A+T L LF+ADIY 
Sbjct: 8   VCVTGGAGYIGSSLVKMLLEKGYTVHATLRNLGDLSKVNFLKSFPKADTRLFLFQADIYN 67

Query: 72  PDDFEDAIRGCQIVFHVATPLQHTHGSQYKNRTEASIATAKNIAKLCVESGTVRRLIYTA 131
           P +FE AI+GC+ VFHVATPLQH  G Q+KN TEA+++  K+IA  C  S TV+RL+YTA
Sbjct: 68  PIEFEHAIQGCKFVFHVATPLQHNQGYQFKNTTEAAVSATKSIATYCQRSKTVKRLVYTA 127

Query: 132 SVVSTSPMKDDGSGFKEFMDETCWTPLH--LSYPFSNTSFEEYIDSKTLTEKVLLEFGES 191
           SVV+ SP++DDGSGFK+ MDETCWT L   +S+P  +   ++Y  SKTL+EK +L +GE+
Sbjct: 128 SVVAASPLRDDGSGFKDSMDETCWTNLDSIISFPDLDDFRKDYTYSKTLSEKEILSYGEN 187

Query: 192 EESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFY---ALRFIEELDGKVP 251
             + ELEVV+LA GL+ GDS  +    T  V  SQ  +     Y   +LR +EE  GKVP
Sbjct: 188 --NNELEVVTLALGLVGGDSPLAYTPATVGVFISQLGNDDVYAYKYQSLRCLEETLGKVP 247

Query: 252 LVHIDDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLHQKHGNSD-EVPT 311
            VHIDD+C AHIFC++  SI GRFLCASS++SS++IA YY  ++P+ +        + P 
Sbjct: 248 AVHIDDVCRAHIFCIQNPSITGRFLCASSYVSSAEIAQYYQENYPEFNATIKQEYLDGPN 307

Query: 312 RNIKMNSKKLIERGFIYKYDDDMILEDAYECYKK--KEMFVNSPVLTPISGGSGFVASSL 371
           R IK  S++LI++GF +KYD   +L+D  +C ++       N      ++G +G++ S L
Sbjct: 308 REIKWGSRRLIDKGFEFKYDLKELLDDCIKCARRTGDRRGFNETKKVCVTGAAGYLGSWL 367

Query: 372 IKTLLQNGHTVHATLRNFNDESKVELLKSLPNATTNLVLFQADIYEPHQFQAAIQGSHVV 431
           +K LL  G+ VHAT RN  D +KV LL SLPNA T L LF+ADIY P +FQ AIQG   V
Sbjct: 368 VKKLLAKGYIVHATTRNLGDPNKVGLLGSLPNAETRLRLFEADIYNPSEFQHAIQGCQFV 427

Query: 432 FHVAT-LFHHTHESQYKNTTEASVAAAKKIAKLCVESRTVRRLIYTGSVVSASPMKEDGS 491
            H+AT L H +  SQ+KNT+EA+VA  K I   C++S TV+RLIYT SVV+ASP+K+DG 
Sbjct: 428 VHMATPLEHQSQSSQFKNTSEAAVAGVKSIVDCCIKSGTVKRLIYTASVVAASPLKQDGK 487

Query: 492 GFKEFMDETCWTPLNLSYPFSNPIFKGYVESKTLTEKELLRFGESEESGGLEVVSLACGV 551
           G K+ +DE+CWT  ++ +P+SN    GY  SKT +EKELL +     +  +E+VSL CG+
Sbjct: 488 GLKDRLDESCWTAKDVVFPYSNEALMGYANSKTASEKELLSY-----NSDIEIVSLVCGL 547

Query: 552 IVGDSLHSYAADSTIVTFSQFTDQTSAFYALRFMEELDGKVPLVHMNDVIEAHIFCMEQS 611
           + G++L S   +S  V  SQ T     +  LRF+EEL GKVP++H+ D  +AHIFC+E+ 
Sbjct: 548 VGGETLLSSIPESMGVLISQATKNKRRYSTLRFLEELLGKVPILHIEDACDAHIFCLEKP 607

Query: 612 SIHGRFFCATSFLSSSDIANYYRLHHPQLHQKHGSVLDSHSLTIVSFVFHLLKCLNSEDV 671
           S+ GRF CA+++LSS+DIA++ +   P +    G V +S                     
Sbjct: 608 SVKGRFLCASAYLSSADIASHCQKLFPDIQIPDGFVENSG-------------------- 667

Query: 672 PIRNIKLDSKKLIDKGFKYKYDGDMILEDALEC 696
             R+I  DS KL + GFKYK+D   +LED++ C
Sbjct: 668 --RDIFWDSAKLKEIGFKYKFDVKTLLEDSINC 671

BLAST of Tan0012214 vs. NCBI nr
Match: KAG6538907.1 (hypothetical protein ZIOFF_004059 [Zingiber officinale])

HSP 1 Score: 606.7 bits (1563), Expect = 3.2e-169
Identity = 358/863 (41.48%), Postives = 484/863 (56.08%), Query Frame = 0

Query: 6   GSCSSTVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLF 65
           G  S  VCVTG +GF+GS L+K+LL  GH VHAT+RN  DESKVG L++L  A   L LF
Sbjct: 4   GRRSCRVCVTGATGFMGSLLVKKLLEKGHVVHATVRNPGDESKVGFLRNLAGAGARLSLF 63

Query: 66  KADIYKPDDFEDAIRGCQIVFHVATPLQHTHGSQYKNRTEASIATAKNIAKLCVESGTVR 125
            AD+Y    F  AI GC+ VF VATP QH   SQ+K+  EA++++ + I +LC  S TVR
Sbjct: 64  SADLYAAHTFAPAIHGCEFVFLVATPFQHIPSSQFKDIAEATVSSVRTILQLCERSRTVR 123

Query: 126 RLIYTASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPFSNTSFEEYIDSKTLTEKVLLE 185
           R+I+T +V ++S MK+DGSGFKE MDE+CWTPLHL +       ++Y  SKTL+EK +L 
Sbjct: 124 RVIFTGTVTASSAMKEDGSGFKEAMDESCWTPLHLPFRHCEDFEKDYTRSKTLSEKEILS 183

Query: 186 FGESEESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRFIEELDGKV 245
           + +      +EVVSL  GL  GD++ S   +++ +  S  T        L+F+  L G V
Sbjct: 184 YNDERRETSMEVVSLTSGLFGGDTILSHLPLSSQIVVSPMTGNPEHRRQLKFLHALLGSV 243

Query: 246 PLVHIDDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHP-QLHQKHGNSDEVP 305
           P+VHIDD+CEAH FCME  S+ GRFLCA+ + +   IA+YY    P QL       DE  
Sbjct: 244 PVVHIDDVCEAHAFCMEPPSMAGRFLCAAGYPALQQIADYYAKKFPDQLPVITKEEDEEA 303

Query: 306 TR-NIKMNSKKLIERGFIYKYDDDMILEDAYECYKKKEMFV-----------------NS 365
            +  +   S KL + GF YK+  + IL+D++E  K+    V                   
Sbjct: 304 AKVYVAGISTKLTDLGFKYKHAVEQILDDSFESAKRTRDVVYQSQSNRDQSIVAMEEGRR 363

Query: 366 PVLTPISGGSGFVASSLIKTLLQNGHTVHATLRNFNDESKVELLKSL--PNATTNLVLFQ 425
                ++G +GF+ S L+K LL+ GH VHAT+RN  DE KV  L++L    A T L LF 
Sbjct: 364 SCRVCVTGATGFMGSLLVKKLLEKGHVVHATVRNPGDEFKVGFLRNLAGAGAGTRLSLFS 423

Query: 426 ADIYEPHQFQAAIQGSHVVFHVATLFHHTHESQYKNTTEASVAAAKKIAKLCVESRTVRR 485
           AD+Y+ H F  AI G   VF VAT F H   SQ+K+  EA+V++ + I +LC  SRTVRR
Sbjct: 424 ADLYDAHTFAQAIHGCEFVFLVATPFQHIPSSQFKDIAEATVSSVRTILQLCERSRTVRR 483

Query: 486 LIYTGSVVSASPMKEDGSGFKEFMDETCWTPLNLSYPFSNPIFKGYVESKTLTEKELLRF 545
           +I+TGSV ++S MKEDGSGFKE MDE+CWTPL+L + +     K Y  SKTL+EKE+L +
Sbjct: 484 VIFTGSVTASSAMKEDGSGFKEAMDESCWTPLHLPFRYCEDSEKDYTRSKTLSEKEILSY 543

Query: 546 GESEESGGLEVVSLACGVIVGDSLHSYAADSTIVTFSQFTDQTSAFYALRFMEELDGKVP 605
            +      +EVVSL  G+  GD++ S+   S+ +  S  T        L+F+  L G VP
Sbjct: 544 NDERRETSMEVVSLTSGLFGGDTILSHLPLSSQIVVSPMTGNPVHRRQLKFLHALLGSVP 603

Query: 606 LVHMNDVIEAHIFCMEQSSIHGRFFCATSFLSSSDIANYYRLHHPQLHQKHGSVLDSHSL 665
           +VH++DV EAH FCME  S+ GRF CA  + +   IA+YY    P              L
Sbjct: 604 VVHIDDVCEAHAFCMEPPSMAGRFLCAAGYPALQQIADYYAKKFPD------------QL 663

Query: 666 TIVSFVFHLLKCLNSEDVPIRNIKLDSK--KLIDKGFKYKYDGDMILEDALECYKKKEMV 725
            +++           ED     + +  K  K+ D GFKYK+  + IL+D+ E  K+    
Sbjct: 664 PVIT---------KEEDEEAAKVYVPGKSTKMTDLGFKYKHTVEQILDDSFESAKR---- 723

Query: 726 NNSIKLSPTSEKDLNEKICMDMPEGNGEKKRFIRIFKEKKEREKKMAVSSFCTVCVTGGS 785
              +     S +D   +  + M EG                          C VCVTG +
Sbjct: 724 TRDVVYQSQSNRD---QSIVAMEEGRRS-----------------------CRVCVTGAT 783

Query: 786 GFVASSLIKRLLLNGHTVHATLRNLNDESKVGLLKSL--PNANTNLVLFKADIYEPDDFE 844
           GF+ S L+K+LL  GH VHAT+RN  DE KVG L++L    A T L LF AD+Y+   F 
Sbjct: 784 GFMGSLLVKKLLEKGHVVHATVRNPGDEFKVGFLRNLAGAGAGTRLSLFSADLYDAHTFA 815

BLAST of Tan0012214 vs. NCBI nr
Match: KAG5579393.1 (hypothetical protein H5410_050020 [Solanum commersonii])

HSP 1 Score: 558.1 bits (1437), Expect = 1.3e-154
Identity = 309/692 (44.65%), Postives = 430/692 (62.14%), Query Frame = 0

Query: 12  VCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFKADIYK 71
           VCVTGG+G++GSSL+K+LL  G+ VHATLRNL DESKVGLLKSL  A+  L LF+ADIYK
Sbjct: 9   VCVTGGAGYIGSSLVKKLLDKGYIVHATLRNLEDESKVGLLKSLSGADKRLKLFEADIYK 68

Query: 72  PDDFEDAIRGCQIVFHVATPLQHTHGSQYKNRTEASIATAKNIAKLCVESGTVRRLIYTA 131
            ++FE+AI+GC+ VFHVATPL H+ GSQYK+RTEA++   K I  +C++SG V+RLIYTA
Sbjct: 69  AEEFEEAIQGCEFVFHVATPLLHSEGSQYKDRTEATVDGVKKIGMICLKSGRVKRLIYTA 128

Query: 132 SVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPFSNTSFEEYIDSKTLTEKVLLEFGESEE 191
           SVV+ SP+K+DG  FK+ MDETCWT L+ S P++     +Y++SK L EK +L+F    E
Sbjct: 129 SVVAASPLKEDGITFKQLMDETCWTSLNFSNPYTKQWLWDYVESKMLAEKEMLKF----E 188

Query: 192 SGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRFIEELDGKVPLVHID 251
              LEVV+L CGL+ G++       +  +  S  T +   + +L+F+EEL GKVP+VHI+
Sbjct: 189 KEGLEVVTLCCGLVGGNTYLPYIPTSGAMFLSMLTQEEILYNSLKFLEELIGKVPIVHIE 248

Query: 252 DICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLHQKHGNSDEVPTRNIKMN 311
           D+CEAH+F M         L  ++ + S                               +
Sbjct: 249 DVCEAHLFFMNNRLA----LTINTIIQS-----------------------------LRS 308

Query: 312 SKKLIERGFIYKYDDDMILEDAYECYKKKEMFVNSPVLTPISGGSGFVASSLIKTLLQNG 371
           +KK+ E   I +              K ++M   S     ++G +G++ SSL+K LL  G
Sbjct: 309 TKKMAEEEEIKQ--------------KTQQMEEKSTYKVCVTGVAGYIGSSLVKKLLDKG 368

Query: 372 HTVHATLRNFNDESKVELLKSLPNATTNLVLFQADIYEPHQFQAAIQGSHVVFHVATLFH 431
           + VHATLRN  DESKV LLKSL  A   L LF+ADIY   +F+ AIQG   VFHVAT   
Sbjct: 369 YIVHATLRNLEDESKVGLLKSLSGADERLKLFEADIYRAEEFEEAIQGYEFVFHVATPLL 428

Query: 432 HTHESQYKNTTEASVAAAKKIAKLCVESRTVRRLIYTGSVVSASPMKEDGSGFKEFMDET 491
           H+  SQYK+ TEA+V   KKI ++C+ S TV+RLIYT S+V+ASP+KEDG  FKE MDET
Sbjct: 429 HSQGSQYKDRTEATVDGVKKIGRICLRSGTVKRLIYTASIVAASPLKEDGITFKELMDET 488

Query: 492 CWTPLNLSYPFSNPIFKGYVESKTLTEKELLRFGESEESGGLEVVSLACGVIVGDSLHSY 551
           CWTPLN S P+++     Y +SK L EKE+L+F    E  GLEVV+L CG++ G +   Y
Sbjct: 489 CWTPLNFSNPYTHQWLWDYTKSKVLAEKEILKF----EKEGLEVVTLCCGLVGGHTYLPY 548

Query: 552 AADSTIVTFSQFTDQTSAFYALRFMEELDGKVPLVHMNDVIEAHIFCMEQ-SSIHGRFFC 611
              ++ +  S FT + + +  L+F+EE++GKVP+VH+ DV EAH+F M    S++GRF C
Sbjct: 549 IPTTSGIFLSVFTQEENLYNKLKFLEEVNGKVPIVHIEDVCEAHLFFMNNVGSLNGRFLC 608

Query: 612 ATSFLSSSDIANYYRLHHPQLHQKHGSVLDSHSLTIVSFVFHLLKCLNSEDVPIRNIKLD 671
           A+SF+S+++I NYY+ ++P+       + D                      P R IK  
Sbjct: 609 ASSFVSTTEIGNYYQHNYPEFKVNQEYLND----------------------PKREIKWG 623

Query: 672 SKKLIDKGFKYKYDGDMILEDALECYKKKEMV 703
           SKKL++KGF +KY    IL+D +   +K +++
Sbjct: 669 SKKLVEKGFVFKYGMKEILDDCVIWARKNDIL 623

BLAST of Tan0012214 vs. ExPASy TrEMBL
Match: A0A4D6KV10 (Anthocyanidin reductase OS=Vigna unguiculata OX=3917 GN=DEO72_LG1g1169 PE=3 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 3.6e-195
Identity = 374/711 (52.60%), Postives = 483/711 (67.93%), Query Frame = 0

Query: 10  STVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFKADI 69
           S VCVTGGSG++GS LI +LL  G+TVHATLR+L +ESKVGLLKSLP+A   LVLF+ADI
Sbjct: 5   SKVCVTGGSGYIGSWLINKLLAKGYTVHATLRDLKNESKVGLLKSLPHAEGKLVLFEADI 64

Query: 70  YKPDDFEDAIRGCQIVFHVATPLQHTHG-SQYKNRTEASIATAKNIAKLCVESGTVRRLI 129
           Y P  F+ AI+GC+ VFHVATPL H  G SQYK+ TEA++A  K+IA  CV SGTV+RLI
Sbjct: 65  YNPTQFDPAIQGCEYVFHVATPLAHEPGSSQYKDTTEAAVAGTKSIAMSCVRSGTVKRLI 124

Query: 130 YTASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPFSNTSFEEYIDSKTLTEKVLLEFGE 189
           YTASVVS +P+K+DG  FK+ MDETCWTPLH S    +  +++YI SKT +EK +L +G 
Sbjct: 125 YTASVVSAAPLKEDGRDFKDVMDETCWTPLHDSLYLHDPFYKDYIFSKTTSEKHVLSYGN 184

Query: 190 SEESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRFIEELDGKVPLV 249
            E  G LEVV+L CGL+ GD+L S+ +  T V  +Q      A+ +L+F+E L GK+PLV
Sbjct: 185 DENGGGLEVVTLPCGLVGGDTLLSTPSTRT-VCIAQIVQNERAYKSLKFLEGLLGKIPLV 244

Query: 250 HIDDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLHQKHGNSDEVPTRNI 309
           HIDD+C+AHIFCME  SI GRFLCASS++S  ++AN+Y L++P+   K  N D    ++I
Sbjct: 245 HIDDVCDAHIFCMETPSITGRFLCASSYISLQEMANHYALYYPEFTVKQENEDG-ERKDI 304

Query: 310 KMNSKKLIERGFIYKYDDDMILEDAYECYKKK-EMFVNSPVLTPI--------------- 369
           K +S KL E+GF+YKYD  MIL+D  +C ++  E++ N     PI               
Sbjct: 305 KWSSTKLCEKGFVYKYDAKMILDDCIKCARRMGELYCNCLWCIPICVKIRKMGEGSKVCV 364

Query: 370 SGGSGFVASSLIKTLLQNGHTVHATLRNFNDESKVELLKSLPNATTNLVLFQADIYEPHQ 429
           +GGSG++ S LI  LL  G+TVHATLR+  +ESKV LLKSLP+A   LVLF+ADIY P Q
Sbjct: 365 TGGSGYIGSWLINKLLAKGYTVHATLRDLKNESKVGLLKSLPHAEGKLVLFEADIYNPTQ 424

Query: 430 FQAAIQGSHVVFHVAT-LFHHTHESQYKNTTEASVAAAKKIAKLCVESRTVRRLIYTGSV 489
           F  AIQG   VFHVAT L H    SQYK+TTEA+VA  K IA  CV S TV+RLIYT SV
Sbjct: 425 FDPAIQGCEYVFHVATPLAHEPGSSQYKDTTEAAVAGTKSIAMSCVRSGTVKRLIYTASV 484

Query: 490 VSASPMKEDGSGFKEFMDETCWTPL--NLSYPFSN-PIFKGYVESKTLTEKELLRFGESE 549
           V+ASP+KEDGS FK+ MDETCWTPL  +L Y F + P++K Y  SKTL+EK LL +G  E
Sbjct: 485 VAASPLKEDGSDFKDVMDETCWTPLHDSLEYLFLDVPLYKDYTYSKTLSEKHLLSYGNDE 544

Query: 550 ESGG-LEVVSLACGVIVGDSLHSYAADSTIVTFSQFTDQTSAFYALRFMEELDGKVPLVH 609
             GG LEVV+LACG++ GD L S A  S ++  +Q       +  L+F+E L GK+PL H
Sbjct: 545 NGGGKLEVVTLACGLVGGDCLVSSALSSRLICIAQIVQNEMTYKTLKFLEGLLGKIPLAH 604

Query: 610 MNDVIEAHIFCMEQSSIHGRFFCATSFLSSSDIANYYRLHHPQLHQKHGSVLDSHSLTIV 669
           ++DV EAHIFCME +S+ GRF CA+S++S  ++AN+Y  +HP+ + K             
Sbjct: 605 IDDVCEAHIFCMESTSVSGRFLCASSYISLQEMANHYARYHPEFNVKQ------------ 664

Query: 670 SFVFHLLKCLNSEDVPIRNIKLDSKKLIDKGFKYKYDGDMILEDALECYKK 699
                       ED   ++IK  S KL DKGF YKYD  MIL+D ++C ++
Sbjct: 665 ----------EDEDGQKKDIKWSSTKLCDKGFVYKYDAKMILDDCIKCARR 691

BLAST of Tan0012214 vs. ExPASy TrEMBL
Match: A0A498KPS8 (Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_009763 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 2.0e-177
Identity = 349/716 (48.74%), Postives = 489/716 (68.30%), Query Frame = 0

Query: 9   SSTVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLP--NANTNLVLFK 68
           ++ VCVTGGS ++GSSLIKRLL  G+TVHATLRNL D  KVGLLK LP   A+  L LF+
Sbjct: 3   NTRVCVTGGSSYLGSSLIKRLLEKGYTVHATLRNLGDPFKVGLLKGLPTLGADNRLKLFE 62

Query: 69  ADIYKPDDFEDAIRGCQIVFHVATPLQH-THGSQYKNRTEASIATAKNIAKLCVESGTVR 128
           ADIY PD+FE AI+GC  +FH+ATPLQH    +Q+K+ +EA+++  K+I + C++SGTV+
Sbjct: 63  ADIYNPDEFETAIQGCNYIFHLATPLQHEPKDTQFKDTSEAAVSGIKSIVRSCIKSGTVK 122

Query: 129 RLIYTASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPFSNTSFEEYIDSKTLTEKVLLE 188
           RLIYTASVV+ SP+KD G+GFK+FMDE+CWTPL+LS+ F+N    +Y  SK+L+EK +L+
Sbjct: 123 RLIYTASVVAASPIKDSGNGFKQFMDESCWTPLNLSFAFANKVLIDYTHSKSLSEKEVLK 182

Query: 189 FGESEESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRFIEELDGKV 248
                 SG+LEVVS+ACGL+ GD+L ++   +  V  SQ T  +  +  LRF+EEL GKV
Sbjct: 183 ----HTSGDLEVVSIACGLVGGDTLLTTMPESMGVLISQITKDSRRYQTLRFLEELLGKV 242

Query: 249 PLVHIDDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLHQKHGNSDEVPT 308
           P+VHI+D+CEAHI CME+ SI+GRFLC S++LSS++IA+++  ++P L        E   
Sbjct: 243 PIVHIEDVCEAHILCMEKPSINGRFLCVSAYLSSAEIASHWEKNYPSLKIAE-EFVEDSG 302

Query: 309 RNIKMNSKKLIERGFIYKYDDDMILEDAYECYKKKEMFVNSP-VLTP---ISGGSGFVAS 368
           R+I   S KL + GF YK+D  +IL+D  +  +    F +S   + P   ++GGS ++ S
Sbjct: 303 RDIGWGSTKLKKIGFEYKFDAKVILDDTLKWAQTMGEFASSQNTVVPKVCVTGGSSYLGS 362

Query: 369 SLIKTLLQNGHTVHATLRNFNDESKVELLKSL--PNATTNLVLFQADIYEPHQFQAAIQG 428
           SLIK LL+ G+TVHATLRN  D  KV LLK L  P A + L LF+ADIY P +F+ AIQG
Sbjct: 363 SLIKGLLEKGYTVHATLRNLGDPFKVGLLKGLPTPGADSRLKLFEADIYNPDEFETAIQG 422

Query: 429 SHVVFHVAT-LFHHTHESQYKNTTEASVAAAKKIAKLCVESRTVRRLIYTGSVVSASPMK 488
            + +FH+AT L H   ++Q+K+T+EA+V+  K I + C++S TV+RLIYT SVV+ASP+K
Sbjct: 423 CNYIFHLATPLQHEPKDTQFKDTSEAAVSGIKSIVRSCIKSGTVKRLIYTASVVAASPIK 482

Query: 489 EDGSGFKEFMDETCWTPLNLSYPFSNPIFKGYVESKTLTEKELLRFGESEESGGLEVVSL 548
           + G+GFK+FMDE+CWTPLNLS+ F+N +   Y  SK+L+EKE+L+      SG LEVVS+
Sbjct: 483 DSGNGFKQFMDESCWTPLNLSFAFANKVLIDYTHSKSLSEKEVLK----HTSGDLEVVSI 542

Query: 549 ACGVIVGDSLHSYAADSTIVTFSQFTDQTSAFYALRFMEELDGKVPLVHMNDVIEAHIFC 608
           ACG++ GD+L +   +S  V  SQ T  +  +  LRF+EEL GKVP+VH+ DV EAHI C
Sbjct: 543 ACGLVGGDTLLTAMPESMGVLISQITKDSRRYQTLRFLEELLGKVPIVHIEDVCEAHILC 602

Query: 609 MEQSSIHGRFFCATSFLSSSDIANYYRLHHPQLHQKHGSVLDSHSLTIVSFVFHLLKCLN 668
           ME+ SI+GRF C +++LSS++IA+++  ++P L      V DS                 
Sbjct: 603 MEKPSINGRFLCVSAYLSSAEIASHWEKNYPSLRITEEFVEDSG---------------- 662

Query: 669 SEDVPIRNIKLDSKKLIDKGFKYKYDGDMILEDALECYKKKEMVNNSIKLSPTSEK 715
                 R+I   S KL   GF+Y +D  +IL+D L+  +K   V   I L P++++
Sbjct: 663 ------RDIGWGSTKLKKIGFEYNFDAKVILDDTLKWAQK--WVPAKILLYPSNDQ 685

BLAST of Tan0012214 vs. ExPASy TrEMBL
Match: A0A1R3H1S3 (NAD-dependent epimerase/dehydratase OS=Corchorus capsularis OX=210143 GN=CCACVL1_21857 PE=4 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 8.7e-173
Identity = 336/693 (48.48%), Postives = 457/693 (65.95%), Query Frame = 0

Query: 12  VCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFKADIYK 71
           VCVTGG+G++GSSL+K LL  G+TVHATLRNL D SKV  LKS P A+T L LF+ADIY 
Sbjct: 8   VCVTGGAGYIGSSLVKMLLEKGYTVHATLRNLGDLSKVNFLKSFPKADTRLFLFQADIYN 67

Query: 72  PDDFEDAIRGCQIVFHVATPLQHTHGSQYKNRTEASIATAKNIAKLCVESGTVRRLIYTA 131
           P +FE AI+GC+ VFHVATPLQH  G Q+KN TEA+++  K+IA  C  S TV+RL+YTA
Sbjct: 68  PIEFEHAIQGCKFVFHVATPLQHNQGYQFKNTTEAAVSATKSIATYCQRSKTVKRLVYTA 127

Query: 132 SVVSTSPMKDDGSGFKEFMDETCWTPLH--LSYPFSNTSFEEYIDSKTLTEKVLLEFGES 191
           SVV+ SP++DDGSGFK+ MDETCWT L   +S+P  +   ++Y  SKTL+EK +L +GE+
Sbjct: 128 SVVAASPLRDDGSGFKDSMDETCWTNLDSIISFPDLDDFRKDYTYSKTLSEKEILSYGEN 187

Query: 192 EESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFY---ALRFIEELDGKVP 251
             + ELEVV+LA GL+ GDS  +    T  V  SQ  +     Y   +LR +EE  GKVP
Sbjct: 188 --NNELEVVTLALGLVGGDSPLAYTPATVGVFISQLGNDDVYAYKYQSLRCLEETLGKVP 247

Query: 252 LVHIDDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLHQKHGNSD-EVPT 311
            VHIDD+C AHIFC++  SI GRFLCASS++SS++IA YY  ++P+ +        + P 
Sbjct: 248 AVHIDDVCRAHIFCIQNPSITGRFLCASSYVSSAEIAQYYQENYPEFNATIKQEYLDGPN 307

Query: 312 RNIKMNSKKLIERGFIYKYDDDMILEDAYECYKK--KEMFVNSPVLTPISGGSGFVASSL 371
           R IK  S++LI++GF +KYD   +L+D  +C ++       N      ++G +G++ S L
Sbjct: 308 REIKWGSRRLIDKGFEFKYDLKELLDDCIKCARRTGDRRGFNETKKVCVTGAAGYLGSWL 367

Query: 372 IKTLLQNGHTVHATLRNFNDESKVELLKSLPNATTNLVLFQADIYEPHQFQAAIQGSHVV 431
           +K LL  G+ VHAT RN  D +KV LL SLPNA T L LF+ADIY P +FQ AIQG   V
Sbjct: 368 VKKLLAKGYIVHATTRNLGDPNKVGLLGSLPNAETRLRLFEADIYNPSEFQHAIQGCQFV 427

Query: 432 FHVAT-LFHHTHESQYKNTTEASVAAAKKIAKLCVESRTVRRLIYTGSVVSASPMKEDGS 491
            H+AT L H +  SQ+KNT+EA+VA  K I   C++S TV+RLIYT SVV+ASP+K+DG 
Sbjct: 428 VHMATPLEHQSQSSQFKNTSEAAVAGVKSIVDCCIKSGTVKRLIYTASVVAASPLKQDGK 487

Query: 492 GFKEFMDETCWTPLNLSYPFSNPIFKGYVESKTLTEKELLRFGESEESGGLEVVSLACGV 551
           G K+ +DE+CWT  ++ +P+SN    GY  SKT +EKELL +     +  +E+VSL CG+
Sbjct: 488 GLKDRLDESCWTAKDVVFPYSNEALMGYANSKTASEKELLSY-----NSDIEIVSLVCGL 547

Query: 552 IVGDSLHSYAADSTIVTFSQFTDQTSAFYALRFMEELDGKVPLVHMNDVIEAHIFCMEQS 611
           + G++L S   +S  V  SQ T     +  LRF+EEL GKVP++H+ D  +AHIFC+E+ 
Sbjct: 548 VGGETLLSSIPESMGVLISQATKNKRRYSTLRFLEELLGKVPILHIEDACDAHIFCLEKP 607

Query: 612 SIHGRFFCATSFLSSSDIANYYRLHHPQLHQKHGSVLDSHSLTIVSFVFHLLKCLNSEDV 671
           S+ GRF CA+++LSS+DIA++ +   P +    G V +S                     
Sbjct: 608 SVKGRFLCASAYLSSADIASHCQKLFPDIQIPDGFVENSG-------------------- 667

Query: 672 PIRNIKLDSKKLIDKGFKYKYDGDMILEDALEC 696
             R+I  DS KL + GFKYK+D   +LED++ C
Sbjct: 668 --RDIFWDSAKLKEIGFKYKFDVKTLLEDSINC 671

BLAST of Tan0012214 vs. ExPASy TrEMBL
Match: A0A0E0QAV4 (Uncharacterized protein OS=Oryza rufipogon OX=4529 PE=4 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 3.9e-165
Identity = 365/871 (41.91%), Postives = 497/871 (57.06%), Query Frame = 0

Query: 7    SCSSTVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFK 66
            S S  VCVTGGSG++ + LIK+LL  G  VHATLRNL DE K  LL+ +P A   LVLF+
Sbjct: 863  SSSRPVCVTGGSGYIATCLIKKLLQRGCGVHATLRNLGDEKKTALLRRMPGAAERLVLFE 922

Query: 67   ADIYKPDDFEDAIRGCQIVFHVATPLQHTH-GSQYKNRTEASIATAKNIAKLCVESGTVR 126
            AD+Y    FE AI GC+ VF +ATPL H    ++YKN TEA++     I + C  S TVR
Sbjct: 923  ADMYDAATFEPAIAGCEFVFLLATPLIHDPLSTKYKNTTEAAVDAMHIILQQCERSKTVR 982

Query: 127  RLIYTASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPFSNTSFEEYIDSKTLTEKVLLE 186
            R+I+TASV + SP+++DG G+K+F++E+CWTPL LS  +SN   + Y+ SKTLTEK+LL 
Sbjct: 983  RVIHTASVTAASPLREDGEGYKDFINESCWTPLDLSNRYSNVMMDAYVSSKTLTEKLLLR 1042

Query: 187  FGESEESGELEVVSLACGLISGDS----LDSSAAVTTIVTFSQFTDQTPAFYALRFIEEL 246
            + ES ES   EVV+L C LI GD+    L  S ++  IV  S  T Q      L+ ++ L
Sbjct: 1043 YNES-ESRAFEVVTLTCALIGGDTDTTQLYHSLSIPLIV--SPLTGQELYHGGLKSLQAL 1102

Query: 247  DGKVPLVHIDDICEAHIFCMEQS-SIHGRFLCASSFLSSSDIANYYHLHHPQLHQKHGNS 306
             G VPL HIDDICEAHIFCMEQ  SI GRFLCA  + +  D  + + + +P++  K    
Sbjct: 1103 LGSVPLAHIDDICEAHIFCMEQQPSIAGRFLCAVGYPNMQDYVDRFAVKYPEIAIKLQGV 1162

Query: 307  DEVPTRNIKMNSKKLIERGFIYKYDDDMILEDAYECYKKKEMFVNS-----------PVL 366
                 R +K ++ KL + GF YK+  +  L+ + E  ++ +   N+           P L
Sbjct: 1163 IGKDVR-VKADTNKLGDLGFKYKFTVEETLDSSVESQQRHQGPSNTSVSDDTPSHTDPFL 1222

Query: 367  TP-----------ISGGSGFVASSLIKTLLQNGHTVHATLRNFNDESKVELLKSLPNATT 426
            T            ++G SG++A+ LIK LLQ G  VHATLRN  DE K   LK LP A  
Sbjct: 1223 TTQMTSSSSSRVCVTGASGYIATCLIKKLLQRGCVVHATLRNLGDEKKTAPLKELPGAVE 1282

Query: 427  NLVLFQADIYEPHQFQAAIQGSHVVFHVATLFHHTHES-QYKNTTEASVAAAKKIAKLCV 486
             LVLF+AD+Y+   F+ AI G   VF +AT  HH   S +YKNTTEA+V A + I + C 
Sbjct: 1283 RLVLFEADMYDADTFEPAIAGCEFVFLLATPLHHDPRSTKYKNTTEAAVDAMRIILQQCE 1342

Query: 487  ESRTVRRLIYTGSVVSASPMKED-GSGFKEFMDETCWTPLNLSYPFSN---PIFKGYVES 546
             S+TVRR+I+T SV +ASP +ED G G+K+F++E+CWTPL+ S+ ++N    +   Y  S
Sbjct: 1343 RSKTVRRVIHTASVTAASPFREDGGEGYKDFINESCWTPLDHSHSYNNTMVDVHNDYSSS 1402

Query: 547  KTLTEKELLRFGESEESGGLEVVSLACGVIVG--DSLHSYAADSTIVTFSQFTDQTSAFY 606
            KTLTEK LLR+ ES ES   EVV+LAC ++ G  D+   Y + S  V  S  T   S   
Sbjct: 1403 KTLTEKLLLRYNES-ESRAFEVVTLACALVGGDADTTQLYHSLSVPVIVSPLTGDESCHN 1462

Query: 607  ALRFMEELDGKVPLVHMNDVIEAHIFCMEQSSIHGRFFCATSFLSSSDIANYYRLHHPQL 666
             L+F++ L G VPL H+ D+ EAHIFC EQ SI GRF CA  + +  D  +++   +P++
Sbjct: 1463 TLKFLQALIGSVPLAHIEDICEAHIFCAEQPSIAGRFLCAVGYPNMQDYVDHFVTKYPEI 1522

Query: 667  HQKHGSVLDSHSLTIVSFVFHLLKCLNSEDVPIRNIKLDSKKLIDKGFKYKYDGDMILED 726
              K                   LK +  +DV    ++ D+ KL+D GFKYKY  +  L  
Sbjct: 1523 TIK-------------------LKEVVGKDV---RVQADTNKLVDLGFKYKYGVEETLIC 1582

Query: 727  ALECYKKKEMVNNSIKLSPTSEKDLNEKICMDMPEGNGEKKRFIRIFKEKKEREKKMAVS 786
            ++EC K+K  ++                                                
Sbjct: 1583 SVECAKRKREMSR----------------------------------------------- 1642

Query: 787  SFCTVCVTGGSGFVASSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFKA 843
                VCVTG +G++A+ L+K+LL  G  VHATLR+L DE K  LL+ +P A   LVLF+A
Sbjct: 1643 ----VCVTGAAGYIATWLVKKLLGRGCVVHATLRDLGDEKKTALLRRMPGAAERLVLFEA 1655

BLAST of Tan0012214 vs. ExPASy TrEMBL
Match: A0A0E0QAV3 (Uncharacterized protein OS=Oryza rufipogon OX=4529 PE=4 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 3.9e-165
Identity = 365/871 (41.91%), Postives = 497/871 (57.06%), Query Frame = 0

Query: 7    SCSSTVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFK 66
            S S  VCVTGGSG++ + LIK+LL  G  VHATLRNL DE K  LL+ +P A   LVLF+
Sbjct: 875  SSSRPVCVTGGSGYIATCLIKKLLQRGCGVHATLRNLGDEKKTALLRRMPGAAERLVLFE 934

Query: 67   ADIYKPDDFEDAIRGCQIVFHVATPLQHTH-GSQYKNRTEASIATAKNIAKLCVESGTVR 126
            AD+Y    FE AI GC+ VF +ATPL H    ++YKN TEA++     I + C  S TVR
Sbjct: 935  ADMYDAATFEPAIAGCEFVFLLATPLIHDPLSTKYKNTTEAAVDAMHIILQQCERSKTVR 994

Query: 127  RLIYTASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPFSNTSFEEYIDSKTLTEKVLLE 186
            R+I+TASV + SP+++DG G+K+F++E+CWTPL LS  +SN   + Y+ SKTLTEK+LL 
Sbjct: 995  RVIHTASVTAASPLREDGEGYKDFINESCWTPLDLSNRYSNVMMDAYVSSKTLTEKLLLR 1054

Query: 187  FGESEESGELEVVSLACGLISGDS----LDSSAAVTTIVTFSQFTDQTPAFYALRFIEEL 246
            + ES ES   EVV+L C LI GD+    L  S ++  IV  S  T Q      L+ ++ L
Sbjct: 1055 YNES-ESRAFEVVTLTCALIGGDTDTTQLYHSLSIPLIV--SPLTGQELYHGGLKSLQAL 1114

Query: 247  DGKVPLVHIDDICEAHIFCMEQS-SIHGRFLCASSFLSSSDIANYYHLHHPQLHQKHGNS 306
             G VPL HIDDICEAHIFCMEQ  SI GRFLCA  + +  D  + + + +P++  K    
Sbjct: 1115 LGSVPLAHIDDICEAHIFCMEQQPSIAGRFLCAVGYPNMQDYVDRFAVKYPEIAIKLQGV 1174

Query: 307  DEVPTRNIKMNSKKLIERGFIYKYDDDMILEDAYECYKKKEMFVNS-----------PVL 366
                 R +K ++ KL + GF YK+  +  L+ + E  ++ +   N+           P L
Sbjct: 1175 IGKDVR-VKADTNKLGDLGFKYKFTVEETLDSSVESQQRHQGPSNTSVSDDTPSHTDPFL 1234

Query: 367  TP-----------ISGGSGFVASSLIKTLLQNGHTVHATLRNFNDESKVELLKSLPNATT 426
            T            ++G SG++A+ LIK LLQ G  VHATLRN  DE K   LK LP A  
Sbjct: 1235 TTQMTSSSSSRVCVTGASGYIATCLIKKLLQRGCVVHATLRNLGDEKKTAPLKELPGAVE 1294

Query: 427  NLVLFQADIYEPHQFQAAIQGSHVVFHVATLFHHTHES-QYKNTTEASVAAAKKIAKLCV 486
             LVLF+AD+Y+   F+ AI G   VF +AT  HH   S +YKNTTEA+V A + I + C 
Sbjct: 1295 RLVLFEADMYDADTFEPAIAGCEFVFLLATPLHHDPRSTKYKNTTEAAVDAMRIILQQCE 1354

Query: 487  ESRTVRRLIYTGSVVSASPMKED-GSGFKEFMDETCWTPLNLSYPFSN---PIFKGYVES 546
             S+TVRR+I+T SV +ASP +ED G G+K+F++E+CWTPL+ S+ ++N    +   Y  S
Sbjct: 1355 RSKTVRRVIHTASVTAASPFREDGGEGYKDFINESCWTPLDHSHSYNNTMVDVHNDYSSS 1414

Query: 547  KTLTEKELLRFGESEESGGLEVVSLACGVIVG--DSLHSYAADSTIVTFSQFTDQTSAFY 606
            KTLTEK LLR+ ES ES   EVV+LAC ++ G  D+   Y + S  V  S  T   S   
Sbjct: 1415 KTLTEKLLLRYNES-ESRAFEVVTLACALVGGDADTTQLYHSLSVPVIVSPLTGDESCHN 1474

Query: 607  ALRFMEELDGKVPLVHMNDVIEAHIFCMEQSSIHGRFFCATSFLSSSDIANYYRLHHPQL 666
             L+F++ L G VPL H+ D+ EAHIFC EQ SI GRF CA  + +  D  +++   +P++
Sbjct: 1475 TLKFLQALIGSVPLAHIEDICEAHIFCAEQPSIAGRFLCAVGYPNMQDYVDHFVTKYPEI 1534

Query: 667  HQKHGSVLDSHSLTIVSFVFHLLKCLNSEDVPIRNIKLDSKKLIDKGFKYKYDGDMILED 726
              K                   LK +  +DV    ++ D+ KL+D GFKYKY  +  L  
Sbjct: 1535 TIK-------------------LKEVVGKDV---RVQADTNKLVDLGFKYKYGVEETLIC 1594

Query: 727  ALECYKKKEMVNNSIKLSPTSEKDLNEKICMDMPEGNGEKKRFIRIFKEKKEREKKMAVS 786
            ++EC K+K  ++                                                
Sbjct: 1595 SVECAKRKREMSR----------------------------------------------- 1654

Query: 787  SFCTVCVTGGSGFVASSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFKA 843
                VCVTG +G++A+ L+K+LL  G  VHATLR+L DE K  LL+ +P A   LVLF+A
Sbjct: 1655 ----VCVTGAAGYIATWLVKKLLGRGCVVHATLRDLGDEKKTALLRRMPGAAERLVLFEA 1667

BLAST of Tan0012214 vs. TAIR 10
Match: AT5G42800.1 (dihydroflavonol 4-reductase )

HSP 1 Score: 163.3 bits (412), Expect = 8.6e-40
Identity = 114/336 (33.93%), Postives = 174/336 (51.79%), Query Frame = 0

Query: 7   SCSSTVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFK 66
           S   TVCVTG SGF+GS L+ RLL  G+ V AT+R+  +  KV  L  LPNA T L L+K
Sbjct: 3   SQKETVCVTGASGFIGSWLVMRLLERGYFVRATVRDPGNLKKVQHLLDLPNAKTLLTLWK 62

Query: 67  ADIYKPDDFEDAIRGCQIVFHVATPLQHTHGSQYKNRTEASIATAKNIAKLCVESGTVRR 126
           AD+ +   ++DAI GC  VFHVATP+            + ++     I K CV++ TVRR
Sbjct: 63  ADLSEEGSYDDAINGCDGVFHVATPMDFESKDPENEVIKPTVNGMLGIMKACVKAKTVRR 122

Query: 127 LIYTASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPFSNTSFEEYIDSKTLTEKVLLEF 186
            ++T+S  + +  +      K   DE  W+ L        T +  Y  SKTL EK   +F
Sbjct: 123 FVFTSSAGTVNVEEHQ----KNVYDENDWSDLEFIMSKKMTGW-MYFVSKTLAEKAAWDF 182

Query: 187 GESEESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRFIEELDGKVP 246
             +EE G L+ +S+   L+ G  + +S   + I   S  T     +  +R       +  
Sbjct: 183 --AEEKG-LDFISIIPTLVVGPFITTSMPPSLITALSPITRNEAHYSIIR-------QGQ 242

Query: 247 LVHIDDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLH--QKHGNSDEVP 306
            VH+DD+C AHIF  EQ++  GR++C+S   +   I+ +    +P+ +        DE  
Sbjct: 243 YVHLDDLCNAHIFLYEQAAAKGRYICSSHDATILTISKFLRPKYPEYNVPSTFEGVDE-N 302

Query: 307 TRNIKMNSKKLIERGFIYKYD-DDMILEDAYECYKK 340
            ++I+ +SKKL + GF +KY  ++M +E    C +K
Sbjct: 303 LKSIEFSSKKLTDMGFNFKYSLEEMFIESIETCRQK 322

BLAST of Tan0012214 vs. TAIR 10
Match: AT4G27250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 160.2 bits (404), Expect = 7.3e-39
Identity = 109/340 (32.06%), Postives = 180/340 (52.94%), Query Frame = 0

Query: 7   SCSSTVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFK 66
           S ++T CVTG SG++GS L+K LL  G+TVHATLR+L   +K    +S    N  L LF+
Sbjct: 8   SKTATYCVTGASGYIGSWLVKSLLQRGYTVHATLRDL---AKSEYFQSKWKENERLRLFR 67

Query: 67  ADIYKPDDFEDAIRGCQIVFHVATPLQHTHGSQYKNR--------TEASIATAKNIAKLC 126
           AD+     F+DA++GC  VFHVA  ++    S + N          E ++   +N+   C
Sbjct: 68  ADLRDDGSFDDAVKGCDGVFHVAASMEFDISSDHVNLESYVQSKVIEPALKGVRNVLSSC 127

Query: 127 VESGTVRRLIYTASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPF-SNTSFEEYIDSKT 186
           ++S +V+R+++T+S +ST   KD+    + F+DETC    H+ +   +  S   Y+ SK 
Sbjct: 128 LKSKSVKRVVFTSS-ISTLTAKDENERMRSFVDETC--KAHVDHVLKTQASGWIYVLSKL 187

Query: 187 LTEKVLLEFGESEESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRF 246
           ++E+    +  ++E G +++VS+    +SG  L      +  V  S  T  +  F  L  
Sbjct: 188 VSEEEAFRY--AKERG-MDLVSVITTTVSGPFLTPFVPSSVQVLLSPITGDSKLFAILSA 247

Query: 247 IEELDGKVPLVHIDDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLHQKH 306
           + +  G + LVHI+DIC AH+F MEQ    G+++C    +   ++  ++         + 
Sbjct: 248 VNKRMGSIALVHIEDICRAHLFLMEQPKAKGQYICCVDNIDMHELMLHHFSKDYLCKVQK 307

Query: 307 GNSDEVPTRNIK--MNSKKLIERGFIYKYDDDMILEDAYE 336
            N DE     +K  ++SKKL E GF YKY  + I++   +
Sbjct: 308 VNEDEEERECMKPIISSKKLRELGFEYKYGIEEIVDQTID 338

BLAST of Tan0012214 vs. TAIR 10
Match: AT1G61720.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 159.1 bits (401), Expect = 1.6e-38
Identity = 101/335 (30.15%), Postives = 177/335 (52.84%), Query Frame = 0

Query: 9   SSTVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFKAD 68
           S   CV GG+G + S LIK LL +G+ V+ T+R+  +E K+  L+ L     +L +FKAD
Sbjct: 10  SKKACVIGGTGNLASILIKHLLQSGYKVNTTVRDPENEKKIAHLRKLQELG-DLKIFKAD 69

Query: 69  IYKPDDFEDAIRGCQIVFHVATPLQHTHGSQYKNRTEASIATAKNIAKLCVESGTVRRLI 128
           +   D FE +  GC+ +FHVATP+        K+  + +I    N+ K C++S +V+R+I
Sbjct: 70  LTDEDSFESSFSGCEYIFHVATPINFKSEDPEKDMIKPAIQGVINVLKSCLKSKSVKRVI 129

Query: 129 YTASVVSTSPMKDDGSGFKEFMDETCWTPLHL---SYPFSNTSFEEYIDSKTLTEKVLLE 188
           YT+S  + S     G+G    M+E  WT +       PF+      Y  SK L EK   E
Sbjct: 130 YTSSAAAVSINNLSGTGI--VMNEENWTDVEFLTEEKPFN----WGYPISKVLAEKTAWE 189

Query: 189 FGESEESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRFIEELDGKV 248
           F    +  ++ +V++   LI+G+SL S    +  ++ S  T +      L+ +++L G +
Sbjct: 190 FA---KENKINLVTVIPALIAGNSLLSDPPSSLSLSMSFITGKEMHVTGLKEMQKLSGSI 249

Query: 249 PLVHIDDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLHQKHGNSDEVPT 308
             VH+DD+  AH+F  E+ +  GR++C +   S  +IA++    +P+ +      + +  
Sbjct: 250 SFVHVDDLARAHLFLAEKETASGRYICCAYNTSVPEIADFLIQRYPKYNVLSEFEEGLSI 309

Query: 309 RNIKMNSKKLIERGFIYKYDDDMILEDAYECYKKK 341
             + ++S+KLI  GF ++Y  + + +   E ++ K
Sbjct: 310 PKLTLSSQKLINEGFRFEYGINEMYDQMIEYFESK 334

BLAST of Tan0012214 vs. TAIR 10
Match: AT4G27250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 150.6 bits (379), Expect = 5.8e-36
Identity = 107/340 (31.47%), Postives = 177/340 (52.06%), Query Frame = 0

Query: 7   SCSSTVCVTGGSGFVGSSLIKRLLLNGHTVHATLRNLNDESKVGLLKSLPNANTNLVLFK 66
           S ++T CVTG SG++GS L+K LL  G+TVHATLR+L   +K    +S    N  L LF+
Sbjct: 8   SKTATYCVTGASGYIGSWLVKSLLQRGYTVHATLRDL---AKSEYFQSKWKENERLRLFR 67

Query: 67  ADIYKPDDFEDAIRGCQIVFHVATPLQHTHGSQYKNR--------TEASIATAKNIAKLC 126
           AD+     F+DA++GC  VFHVA  ++    S + N          E ++   +N+   C
Sbjct: 68  ADLRDDGSFDDAVKGCDGVFHVAASMEFDISSDHVNLESYVQSKVIEPALKGVRNVLSSC 127

Query: 127 VESGTVRRLIYTASVVSTSPMKDDGSGFKEFMDETCWTPLHLSYPF-SNTSFEEYIDSKT 186
           ++S +V+R+++T+S +ST   KD+    + F+DETC    H+ +   +  S   Y+ SK 
Sbjct: 128 LKSKSVKRVVFTSS-ISTLTAKDENERMRSFVDETC--KAHVDHVLKTQASGWIYVLSKL 187

Query: 187 LTEKVLLEFGESEESGELEVVSLACGLISGDSLDSSAAVTTIVTFSQFTDQTPAFYALRF 246
           ++E+    +  ++E G +++VS+    +SG  L      +  V  S  T           
Sbjct: 188 VSEEEAFRY--AKERG-MDLVSVITTTVSGPFLTPFVPSSVQVLLSPIT----------A 247

Query: 247 IEELDGKVPLVHIDDICEAHIFCMEQSSIHGRFLCASSFLSSSDIANYYHLHHPQLHQKH 306
           + +  G + LVHI+DIC AH+F MEQ    G+++C    +   ++  ++         + 
Sbjct: 248 VNKRMGSIALVHIEDICRAHLFLMEQPKAKGQYICCVDNIDMHELMLHHFSKDYLCKVQK 307

Query: 307 GNSDEVPTRNIK--MNSKKLIERGFIYKYDDDMILEDAYE 336
            N DE     +K  ++SKKL E GF YKY  + I++   +
Sbjct: 308 VNEDEEERECMKPIISSKKLRELGFEYKYGIEEIVDQTID 328

BLAST of Tan0012214 vs. TAIR 10
Match: AT2G45400.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 137.5 bits (345), Expect = 5.1e-32
Identity = 108/352 (30.68%), Postives = 163/352 (46.31%), Query Frame = 0

Query: 349 LTPISGGSGFVASSLIKTLLQNGHTVHATLRNFNDESK--VELLKSLPNATTNLVLFQAD 408
           L  ++GGSGFVAS LI  LLQ G++V AT+R  ++ +K  +  L  LP A+  L +F AD
Sbjct: 39  LVCVTGGSGFVASWLIMRLLQRGYSVRATVRTNSEGNKKDISYLTELPFASERLQIFTAD 98

Query: 409 IYEPHQFQAAIQGSHVVFHVATLFHHTHESQYKNTTEASVAAAKKIAKLCVESRTVRRLI 468
           + EP  F+ AI+G   VFHVA           +  T+ +V     I K C++++TV+R  
Sbjct: 99  LNEPESFKPAIEGCKAVFHVAHPMDPNSNETEETVTKRTVQGLMGILKSCLDAKTVKRFF 158

Query: 469 YTGSVVSASPMKEDGSGFKEFMDETCWTPLNLSYPFSNP----IFKGYVESKTLTEKELL 528
           YT S V+      +G G  E +DE+ W+ + +   F N     +   YV SK   E   L
Sbjct: 159 YTSSAVTVFYSGGNGGGGGE-VDESVWSDVEV---FRNQKEKRVSSSYVVSKMAAETAAL 218

Query: 529 RFGESEESGGLEVVSLACGVIVGDSLHSYAADSTIVTFSQFTDQTSAFYALRFMEELDGK 588
            FG      GLEVV+L   ++VG  + S    S  ++ +         Y       L   
Sbjct: 219 EFG---GKNGLEVVTLVIPLVVGPFISSSLPSSVFISLAMLFGNYKEKY-------LFDT 278

Query: 589 VPLVHMNDVIEAHIFCMEQSSIHGRFFCATSFLSSSDIANYYRLHHPQLHQKHGSVLDSH 648
             +VH++DV  A IF +E+    GR+ C++  +   ++  +     PQ            
Sbjct: 279 YNMVHIDDVARAMIFLLEKPVAKGRYICSSVEMKIDEVFEFLSTKFPQ------------ 338

Query: 649 SLTIVSFVFHLLKC-LNSEDVPIRNIKLDSKKLIDKGFKYKYDGDMILEDAL 694
                   F L    LN   V  + + L SKKL   GF++KY  + I   A+
Sbjct: 339 --------FQLPSIDLNKYKVE-KRMGLSSKKLKSAGFEFKYGAEEIFSGAI 355