Tan0012178 (gene) Snake gourd v1

Overview


Name	Tan0012178
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Calcium-binding EF-hand family protein
Location	LG05: 75481562 .. 75482712 (-)
RNA-Seq Expression	Tan0012178
Synteny	Tan0012178

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGATGACAGAGTCTCCTTTAGCCGCGGCAGATACGGATCGTCCAAGTTTCAAGAAGATGATACGAAATTTCTCAGAACTTCATAAAACTTCGTTCCTTCTGAAGAAGCCGATGAAGAAAGACAAAATAAAGTCATTGCTTTCACTCCAATGTTCTAATTTCCTCGAAAGTTTCACTGTCGACGCTTAAACACGCAATATGTAGATTAAAGCGCAATAACGCTCGTACATTTGCAGAAGTTTAATCAGCCAAGACTTGACCGCTCACTCAAATATAAATTGGTAATCCATGAGAACTCTTCTCTTGTCTTCTGCTGACTGTTTAATTAGCTTTATAGCACTCATATATACATACTTTTTGTTCACTCTTTCAAACATGGCACTAATTGATGATAACCCCATTTCAGGTTTTGAGATTTTGGTTCTCATTTTTCTCAATTTCATACTTGAACGTTTCTTTTCAGGTTTATTGTACATTTTTATCCTCTGTGCTGCGTTTCTTTTTTATCTTTGGAATTCTTGGGTTGCCCACCGCAACATCATTAAACGTCTTGATGCTGCTGAAGCGGCATGCACACCAAGTGTTCGACGAAATGCTCATGAGGGGCATTACTACACAGAACTTGTGGACGTGCCCGTTAATGACAAACACGAAGTGAAACTGACTATTGAAGATGTGAAAACTATGATGGAGGCACTTGATCGTGGCCTTAATAACAATGAAAATAATAATCTTGGGTTGAATGCTGAGATTGAGGGACTATTTGAGGAAGAATTGAGTTTAGATGAAGTTGAGGAAGCTTTTGATTTGTTTGATGAGAATGGTGATGGGTTTATTGATGCAGAGGACTTGAAGAAGGTGCTGTGTGCCTTGGGATTTACAGTGGCTTCAGAAGTAGATCAATGCAGGAGAATGATTAGAGTATTTGACGATGATGGAGATGGGCGTTTAGATTTTAAAGAATTTGCCAAGCTTGTTGAACAAAGCTTTGGTTGAAAACTACTCTGTGTTTGAAGCAAGTATCCCCATTTGTAAGAAAACTGATAGCTTCAACCAATTGTATTAAAATTTTATTGTAATTTGTACTTGAAATGCAAGTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT

mRNA sequence

AGATGACAGAGTCTCCTTTAGCCGCGGCAGATACGGATCGTCCAAGTTTCAAGAAGATGATACGAAATTTCTCAGAACTTCATAAAACTTCGTTCCTTCTGAAGAAGCCGATGAAGAAAGACAAAATAAAGTCATTGCTTTCACTCCAATGTTCTAATTTCCTCGAAAGTTTCACTGTCGACGCTTAAACACGCAATATGTAGATTAAAGCGCAATAACGCTCGTACATTTGCAGAAGTTTAATCAGCCAAGACTTGACCGCTCACTCAAATATAAATTGGTAATCCATGAGAACTCTTCTCTTGTCTTCTGCTGACTGTTTAATTAGCTTTATAGCACTCATATATACATACTTTTTGTTCACTCTTTCAAACATGGCACTAATTGATGATAACCCCATTTCAGGTTTTGAGATTTTGGTTCTCATTTTTCTCAATTTCATACTTGAACGTTTCTTTTCAGGTTTATTGTACATTTTTATCCTCTGTGCTGCGTTTCTTTTTTATCTTTGGAATTCTTGGGTTGCCCACCGCAACATCATTAAACGTCTTGATGCTGCTGAAGCGGCATGCACACCAAGTGTTCGACGAAATGCTCATGAGGGGCATTACTACACAGAACTTGTGGACGTGCCCGTTAATGACAAACACGAAGTGAAACTGACTATTGAAGATGTGAAAACTATGATGGAGGCACTTGATCGTGGCCTTAATAACAATGAAAATAATAATCTTGGGTTGAATGCTGAGATTGAGGGACTATTTGAGGAAGAATTGAGTTTAGATGAAGTTGAGGAAGCTTTTGATTTGTTTGATGAGAATGGTGATGGGTTTATTGATGCAGAGGACTTGAAGAAGGTGCTGTGTGCCTTGGGATTTACAGTGGCTTCAGAAGTAGATCAATGCAGGAGAATGATTAGAGTATTTGACGATGATGGAGATGGGCGTTTAGATTTTAAAGAATTTGCCAAGCTTGTTGAACAAAGCTTTGGTTGAAAACTACTCTGTGTTTGAAGCAAGTATCCCCATTTGTAAGAAAACTGATAGCTTCAACCAATTGTATTAAAATTTTATTGTAATTTGTACTTGAAATGCAAGTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT

Coding sequence (CDS)

ATGAGAACTCTTCTCTTGTCTTCTGCTGACTGTTTAATTAGCTTTATAGCACTCATATATACATACTTTTTGTTCACTCTTTCAAACATGGCACTAATTGATGATAACCCCATTTCAGGTTTTGAGATTTTGGTTCTCATTTTTCTCAATTTCATACTTGAACGTTTCTTTTCAGGTTTATTGTACATTTTTATCCTCTGTGCTGCGTTTCTTTTTTATCTTTGGAATTCTTGGGTTGCCCACCGCAACATCATTAAACGTCTTGATGCTGCTGAAGCGGCATGCACACCAAGTGTTCGACGAAATGCTCATGAGGGGCATTACTACACAGAACTTGTGGACGTGCCCGTTAATGACAAACACGAAGTGAAACTGACTATTGAAGATGTGAAAACTATGATGGAGGCACTTGATCGTGGCCTTAATAACAATGAAAATAATAATCTTGGGTTGAATGCTGAGATTGAGGGACTATTTGAGGAAGAATTGAGTTTAGATGAAGTTGAGGAAGCTTTTGATTTGTTTGATGAGAATGGTGATGGGTTTATTGATGCAGAGGACTTGAAGAAGGTGCTGTGTGCCTTGGGATTTACAGTGGCTTCAGAAGTAGATCAATGCAGGAGAATGATTAGAGTATTTGACGATGATGGAGATGGGCGTTTAGATTTTAAAGAATTTGCCAAGCTTGTTGAACAAAGCTTTGGTTGA

Protein sequence

MRTLLLSSADCLISFIALIYTYFLFTLSNMALIDDNPISGFEILVLIFLNFILERFFSGLLYIFILCAAFLFYLWNSWVAHRNIIKRLDAAEAACTPSVRRNAHEGHYYTELVDVPVNDKHEVKLTIEDVKTMMEALDRGLNNNENNNLGLNAEIEGLFEEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEVDQCRRMIRVFDDDGDGRLDFKEFAKLVEQSFG

Homology

[Close]

BLAST of Tan0012178 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.9e-18
Identity = 51/118 (43.22%), Postives = 76/118 (64.41%), Query Frame = 0


Query: 118 NDKHEVKLTIEDVKTMMEALDRGLNNNENNNLGLNAEIEGLFEE-ELSLDEVEEAFDLFD 177
           +D  ++ ++ E+ + +M +L    N+++        E+  LFEE E SL+EV++AFD+FD
Sbjct: 76  DDDDDIDISREEAEMVMRSLGLFYNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVFD 135

Query: 178 ENGDGFIDAEDLKKVLCALGFTVASEVDQCRRMIRVFDDDGDGRLDFKEFAKLVEQSF 235
           EN DGFIDA +L++VL  LGF   S +D C  MIR  D + DG++DF EF K +E SF
Sbjct: 136 ENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETSF 193

[Close]

BLAST of Tan0012178 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.9e-18
Identity = 59/133 (44.36%), Postives = 81/133 (60.90%), Query Frame = 0


Query: 108 YYTELVDVPVNDKHEVKLTIEDVKTMMEALDRGLNNNENNNLGL-----NAEIEGLFEE- 167
           + T +      D  +  L  EDV  +M++L  GL+ ++ N  GL     + E+  LFEE 
Sbjct: 74  FQTSIKHEEYRDDDDDGLCREDVGMVMKSL--GLSTDQENE-GLQKQYSSKEVSNLFEEK 133

Query: 168 ELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEVDQCRRMIRVFDDDGDGRL 227
           E SL+EV++AFD+FDEN DGFID  DL++VL  LG    S ++ CRRMIR FD   DGR+
Sbjct: 134 EPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRI 193

Query: 228 DFKEFAKLVEQSF 235
           DF  F K +E +F
Sbjct: 194 DFYGFVKFMENNF 203

[Close]

BLAST of Tan0012178 vs. ExPASy Swiss-Prot
Match: Q2QVI1 (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.9e-13
Identity = 33/63 (52.38%), Postives = 46/63 (73.02%), Query Frame = 0


Query: 168 VEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEVDQCRRMIRVFDDDGDGRLDFKEFA 227
           + EAF++FD+NGDGFI  ++L+ VL +LG       D CRRMI + D DGDGR+DFKEF 
Sbjct: 100 MREAFNVFDQNGDGFITVDELRSVLSSLGLKHGRTADDCRRMISMVDADGDGRVDFKEFK 159

Query: 228 KLV 231
           +++
Sbjct: 160 QMM 162

[Close]

BLAST of Tan0012178 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 9.1e-13
Identity = 32/72 (44.44%), Postives = 54/72 (75.00%), Query Frame = 0


Query: 160 EEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEVDQCRRMIRVFDDDGDG 219
           EE+   ++++EAF++FD+NGDGFI  ++LK VL +LG      +D C++MI+  D DGDG
Sbjct: 72  EEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDG 131

Query: 220 RLDFKEFAKLVE 232
           R+++KEF ++++
Sbjct: 132 RVNYKEFRQMMK 143

[Close]

BLAST of Tan0012178 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.6e-12
Identity = 64/207 (30.92%), Postives = 107/207 (51.69%), Query Frame = 0


Query: 34  DDNPISGFEILVLIFLNFILERFFSGLLYIFILCAAFLFYLW--NSWVAHRNIIKRLDAA 93
           D + +S   +L ++   FIL      ++  F     F F+L+  N+++   +I       
Sbjct: 3   DSSLLSPISLLTIVIFLFILNLMM--IIQDFSSSFPFRFHLFFSNAYILFTSI------- 62

Query: 94  EAACTPSVRRNAHEGHYYTELVDVPVNDKHEVKLTIEDVKTMM---EALDRGL-NNNENN 153
                    RN  +      +  V V ++ ++K ++E+VK ++   EAL   L    E  
Sbjct: 63  ---------RNNKQNTELPIIKKVVVPNRADIKTSVEEVKAIIDDSEALYECLIEEGEEY 122

Query: 154 NLGLNAEIEGLFEEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEVDQCR 213
            L  N        E +  + V+EAF LFDEN DGFID  +LK VL  LG+   +++ +CR
Sbjct: 123 LLEKN--------EMMGKEIVKEAFRLFDENQDGFIDENELKHVLSLLGYDECTKM-ECR 182

Query: 214 RMIRVFDDDGDGRLDFKEFAKLVEQSF 235
           +M++V+D++ DG++DF EF KL+E+SF
Sbjct: 183 KMVKVYDENRDGKIDFYEFVKLIEKSF 182

[Close]

BLAST of Tan0012178 vs. NCBI nr
Match: XP_038895011.1 (probable calcium-binding protein CML45 [Benincasa hispida])

HSP 1 Score: 310.8 bits (795), Expect = 9.8e-81
Identity = 162/209 (77.51%), Postives = 179/209 (85.65%), Query Frame = 0


Query: 30  MALIDD----NPISGFEILVLIFLNFILERFFSGLLYIFILCAAFLFYLWNSWVAHRNII 89
           MA ID+    +PIS F+IL LIFLNF+LERFF GLLY+FILCAAFLFYL NSW AHRNII
Sbjct: 1   MAPIDELDYASPISVFQILFLIFLNFVLERFFPGLLYVFILCAAFLFYLCNSWAAHRNII 60

Query: 90  KRLDAAEAACTPSVRRNAHEGHYYTELVDVPVNDKHEVKLTIEDVKTMMEALDRGLNNNE 149
           KRL+AA+A CTPSVRRNAHEGH YT++V +PVND  EVKLTIEDVKTMME LD   NN E
Sbjct: 61  KRLEAADATCTPSVRRNAHEGHCYTDIVVMPVNDTKEVKLTIEDVKTMMETLDHEHNNAE 120

Query: 150 NNNLGLNAEIEGLFEEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEVDQ 209
           +NNL LNAEI GLFEEELSL EV+EAFDLFD+NGDGFID EDLKKVLC +GF VA EVDQ
Sbjct: 121 SNNLELNAEIVGLFEEELSLGEVKEAFDLFDDNGDGFIDVEDLKKVLCGMGFAVALEVDQ 180

Query: 210 CRRMIRVFDDDGDGRLDFKEFAKLVEQSF 235
           CRRMI  FD +GDGR+DF+EFAKLVEQSF
Sbjct: 181 CRRMISGFDSNGDGRIDFEEFAKLVEQSF 209

[Close]

BLAST of Tan0012178 vs. NCBI nr
Match: XP_023001351.1 (probable calcium-binding protein CML47 [Cucurbita maxima])

HSP 1 Score: 301.6 bits (771), Expect = 5.9e-78
Identity = 163/215 (75.81%), Postives = 179/215 (83.26%), Query Frame = 0


Query: 25  FTLSNMALIDD----NPISGFEILVLIFLNFILERFFSGLLYIFILCAAFLFYLWNSWVA 84
           FTLS MA ID+    NPIS F+IL L FLNF+LE  F G LY+FILCAAF FYLWNSWVA
Sbjct: 7   FTLSQMAPIDELDYANPISVFQILFLFFLNFLLEYLFPGPLYVFILCAAFHFYLWNSWVA 66

Query: 85  HRNIIKRLDAA-EAACTPSVRRNAHEGHYYTELVDVPVNDKHEVKLTIEDVKTMMEALDR 144
           HRNIIKR +AA +A CTPS RRNAH GH YT +V +PVND HEVKLTIEDVKTMM+AL  
Sbjct: 67  HRNIIKRFEAADDATCTPSARRNAHMGHCYTGIVGMPVNDAHEVKLTIEDVKTMMDALH- 126

Query: 145 GLNNNENNNLGLNAEIEGLFEEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTV 204
             NN E NNL L AEIEG+FEEELS+ EVEEAFDLFDENGDGFIDAEDLKKVL A+GFTV
Sbjct: 127 --NNMETNNLELKAEIEGVFEEELSVGEVEEAFDLFDENGDGFIDAEDLKKVLDAMGFTV 186

Query: 205 ASEVDQCRRMIRVFDDDGDGRLDFKEFAKLVEQSF 235
           ASEVD+CRRMI  +D +GDGRLDF+EFAKLVEQSF
Sbjct: 187 ASEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSF 218

[Close]

BLAST of Tan0012178 vs. NCBI nr
Match: XP_023519469.1 (probable calcium-binding protein CML47 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 295.8 bits (756), Expect = 3.3e-76
Identity = 160/215 (74.42%), Postives = 179/215 (83.26%), Query Frame = 0


Query: 25  FTLSNMALIDD----NPISGFEILVLIFLNFILERFFSGLLYIFILCAAFLFYLWNSWVA 84
           FTLS MA ID+    NPIS F+IL L FLNF+LE  F G LY+FILCAAF FYLWNSWVA
Sbjct: 7   FTLSQMAPIDELDYANPISVFQILFLFFLNFLLEYLFPGPLYVFILCAAFHFYLWNSWVA 66

Query: 85  HRNIIKRLDAA-EAACTPSVRRNAHEGHYYTELVDVPVNDKHEVKLTIEDVKTMMEALDR 144
           HRNIIKR +AA +A CTPS RRNAH GH YT +V +PVND +EVKLTI+DVKTMMEAL  
Sbjct: 67  HRNIIKRFEAADDATCTPSARRNAHVGHCYTGIVGMPVNDAYEVKLTIDDVKTMMEALH- 126

Query: 145 GLNNNENNNLGLNAEIEGLFEEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTV 204
             NN E +NL L AEIEG+FEEELS+ EV+EAFDLFDENGDGFIDAEDLKKVL A+GFTV
Sbjct: 127 --NNMETSNLELKAEIEGVFEEELSVGEVKEAFDLFDENGDGFIDAEDLKKVLDAMGFTV 186

Query: 205 ASEVDQCRRMIRVFDDDGDGRLDFKEFAKLVEQSF 235
           ASEVD+CRRMI  +D +GDGRLDF+EFAKLVEQSF
Sbjct: 187 ASEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSF 218

[Close]

BLAST of Tan0012178 vs. NCBI nr
Match: KAG7020054.1 (putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 293.1 bits (749), Expect = 2.1e-75
Identity = 158/210 (75.24%), Postives = 175/210 (83.33%), Query Frame = 0


Query: 30  MALIDD----NPISGFEILVLIFLNFILERFFSGLLYIFILCAAFLFYLWNSWVAHRNII 89
           MA ID+    NPIS F+IL L FLNF+LE  F G LY+F+LCAAF FYLWNSWVAHRNII
Sbjct: 1   MAPIDELDYANPISVFQILFLFFLNFLLEYLFPGPLYVFMLCAAFHFYLWNSWVAHRNII 60

Query: 90  KRLDAAE-AACTPSVRRNAHEGHYYTELVDVPVNDKHEVKLTIEDVKTMMEALDRGLNNN 149
           KR +AA+ A CTPS RRNAH GH YT +V +PVND HEVKLTIEDVKTMMEAL    NN 
Sbjct: 61  KRFEAADGATCTPSARRNAHVGHCYTGIVGMPVNDAHEVKLTIEDVKTMMEALH---NNM 120

Query: 150 ENNNLGLNAEIEGLFEEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEVD 209
           E NNL L AEIEG+FEEELS+ EV+EAFDLFDENGDGFIDAEDLKKVL A+GFTVASEVD
Sbjct: 121 ETNNLELKAEIEGVFEEELSVGEVKEAFDLFDENGDGFIDAEDLKKVLDAMGFTVASEVD 180

Query: 210 QCRRMIRVFDDDGDGRLDFKEFAKLVEQSF 235
           +CRRMI  +D +GDGRLDF+EFAKLVEQSF
Sbjct: 181 ECRRMISGYDKNGDGRLDFEEFAKLVEQSF 207

[Close]

BLAST of Tan0012178 vs. NCBI nr
Match: KAA0036089.1 (putative calcium-binding protein CML30 [Cucumis melo var. makuwa] >TYJ98897.1 putative calcium-binding protein CML30 [Cucumis melo var. makuwa])

HSP 1 Score: 290.0 bits (741), Expect = 1.8e-74
Identity = 149/200 (74.50%), Postives = 170/200 (85.00%), Query Frame = 0


Query: 36  NPISGFEILVLIFLNFILERFFSGLLYIFILCAAFLFYLWNSWVAHRNIIKRLDAAEAAC 95
           NPIS F IL L FLNFILERFF GL YIFIL AAFLFYLWNSW A RNII+RL+AA+  C
Sbjct: 11  NPISVFHILCLFFLNFILERFFPGLFYIFILYAAFLFYLWNSWAAQRNIIRRLEAADVTC 70

Query: 96  TPSVRRNAHEGHYYTELVDVPVNDKHEVKLTIEDVKTMMEALDRGLNNN-ENNNLGLNAE 155
           TPSVR+N HE H  T++V  PVNDK EVKL+IEDVKTMME LD   NNN +++NLGL+AE
Sbjct: 71  TPSVRQNDHEEHCCTKIVGTPVNDKREVKLSIEDVKTMMETLDHEHNNNRDSDNLGLSAE 130

Query: 156 IEGLFEEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEVDQCRRMIRVFD 215
           +E +FEEELSL EV+EAFDLFDENGDGFIDAEDL+KVLC LGF VAS++DQC+ MI  FD
Sbjct: 131 LEEMFEEELSLGEVQEAFDLFDENGDGFIDAEDLEKVLCGLGFAVASDIDQCKTMISGFD 190

Query: 216 DDGDGRLDFKEFAKLVEQSF 235
           ++GDGRLDF+EFAKLVEQSF
Sbjct: 191 NNGDGRLDFEEFAKLVEQSF 210

[Close]

BLAST of Tan0012178 vs. ExPASy TrEMBL
Match: A0A6J1KID5 (probable calcium-binding protein CML47 OS=Cucurbita maxima OX=3661 GN=LOC111495509 PE=4 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 2.9e-78
Identity = 163/215 (75.81%), Postives = 179/215 (83.26%), Query Frame = 0


Query: 25  FTLSNMALIDD----NPISGFEILVLIFLNFILERFFSGLLYIFILCAAFLFYLWNSWVA 84
           FTLS MA ID+    NPIS F+IL L FLNF+LE  F G LY+FILCAAF FYLWNSWVA
Sbjct: 7   FTLSQMAPIDELDYANPISVFQILFLFFLNFLLEYLFPGPLYVFILCAAFHFYLWNSWVA 66

Query: 85  HRNIIKRLDAA-EAACTPSVRRNAHEGHYYTELVDVPVNDKHEVKLTIEDVKTMMEALDR 144
           HRNIIKR +AA +A CTPS RRNAH GH YT +V +PVND HEVKLTIEDVKTMM+AL  
Sbjct: 67  HRNIIKRFEAADDATCTPSARRNAHMGHCYTGIVGMPVNDAHEVKLTIEDVKTMMDALH- 126

Query: 145 GLNNNENNNLGLNAEIEGLFEEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTV 204
             NN E NNL L AEIEG+FEEELS+ EVEEAFDLFDENGDGFIDAEDLKKVL A+GFTV
Sbjct: 127 --NNMETNNLELKAEIEGVFEEELSVGEVEEAFDLFDENGDGFIDAEDLKKVLDAMGFTV 186

Query: 205 ASEVDQCRRMIRVFDDDGDGRLDFKEFAKLVEQSF 235
           ASEVD+CRRMI  +D +GDGRLDF+EFAKLVEQSF
Sbjct: 187 ASEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSF 218

[Close]

BLAST of Tan0012178 vs. ExPASy TrEMBL
Match: A0A5A7T3X0 (Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00940 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 8.6e-75
Identity = 149/200 (74.50%), Postives = 170/200 (85.00%), Query Frame = 0


Query: 36  NPISGFEILVLIFLNFILERFFSGLLYIFILCAAFLFYLWNSWVAHRNIIKRLDAAEAAC 95
           NPIS F IL L FLNFILERFF GL YIFIL AAFLFYLWNSW A RNII+RL+AA+  C
Sbjct: 11  NPISVFHILCLFFLNFILERFFPGLFYIFILYAAFLFYLWNSWAAQRNIIRRLEAADVTC 70

Query: 96  TPSVRRNAHEGHYYTELVDVPVNDKHEVKLTIEDVKTMMEALDRGLNNN-ENNNLGLNAE 155
           TPSVR+N HE H  T++V  PVNDK EVKL+IEDVKTMME LD   NNN +++NLGL+AE
Sbjct: 71  TPSVRQNDHEEHCCTKIVGTPVNDKREVKLSIEDVKTMMETLDHEHNNNRDSDNLGLSAE 130

Query: 156 IEGLFEEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEVDQCRRMIRVFD 215
           +E +FEEELSL EV+EAFDLFDENGDGFIDAEDL+KVLC LGF VAS++DQC+ MI  FD
Sbjct: 131 LEEMFEEELSLGEVQEAFDLFDENGDGFIDAEDLEKVLCGLGFAVASDIDQCKTMISGFD 190

Query: 216 DDGDGRLDFKEFAKLVEQSF 235
           ++GDGRLDF+EFAKLVEQSF
Sbjct: 191 NNGDGRLDFEEFAKLVEQSF 210

[Close]

BLAST of Tan0012178 vs. ExPASy TrEMBL
Match: A0A1S3BEM1 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103488781 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 5.6e-74
Identity = 148/200 (74.00%), Postives = 169/200 (84.50%), Query Frame = 0


Query: 36  NPISGFEILVLIFLNFILERFFSGLLYIFILCAAFLFYLWNSWVAHRNIIKRLDAAEAAC 95
           NPIS F IL L FLNFILERFF GL YIFIL AAFLFYLWNSW A RNII+RL+AA+  C
Sbjct: 11  NPISVFHILCLFFLNFILERFFPGLFYIFILYAAFLFYLWNSWAAQRNIIRRLEAADVTC 70

Query: 96  TPSVRRNAHEGHYYTELVDVPVNDKHEVKLTIEDVKTMMEALDRGLNNN-ENNNLGLNAE 155
           TPSVR+N HE H  T++V  PVNDK EVKL+IEDVKTMME LD   NNN +++NLGL+AE
Sbjct: 71  TPSVRQNDHEEHCCTKIVGTPVNDKREVKLSIEDVKTMMETLDHEHNNNRDSDNLGLSAE 130

Query: 156 IEGLFEEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEVDQCRRMIRVFD 215
           +E +FEEELSL EV+EAFDLFDEN DGFIDAEDL+KVLC LGF VAS++DQC+ MI  FD
Sbjct: 131 LEEMFEEELSLGEVQEAFDLFDENDDGFIDAEDLEKVLCGLGFAVASDIDQCKTMISGFD 190

Query: 216 DDGDGRLDFKEFAKLVEQSF 235
           ++GDGRLDF+EFAKLVEQSF
Sbjct: 191 NNGDGRLDFEEFAKLVEQSF 210

[Close]

BLAST of Tan0012178 vs. ExPASy TrEMBL
Match: A0A6J1C8B5 (uncharacterized protein LOC111008903 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008903 PE=4 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 5.6e-66
Identity = 139/186 (74.73%), Postives = 160/186 (86.02%), Query Frame = 0


Query: 30  MALIDD----NPISGFEILVLIFLNFILERFFSGLLYIFILCAAFLFYLWNSWVAHRNII 89
           M  IDD      IS  EIL+LIFLN +LE FFSGLLYIFILCAAFLFYLW SWVAHRN +
Sbjct: 1   MEPIDDIEYAESISASEILLLIFLNIVLEHFFSGLLYIFILCAAFLFYLWKSWVAHRNFV 60

Query: 90  KRLDAAEAA-CTPSVRRNAHEGHYYTELVDVPVNDKHEVKLTIEDVKTMMEALDRGLN-N 149
           +RLDAA++A C  SVR+NA+E HY TE+VDVP+ND  EVKL+IEDVKTMMEALDR  + N
Sbjct: 61  QRLDAADSATCMRSVRQNAYEEHYPTEIVDVPINDTREVKLSIEDVKTMMEALDREHDCN 120

Query: 150 NENNNLGLNAEIEGLFEEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEV 209
           NENNNLGL+AEIEGLFEEE+SL EV+EAFDLFDENGDGFIDAE+LKKVLCA+GFTVAS +
Sbjct: 121 NENNNLGLSAEIEGLFEEEVSLGEVKEAFDLFDENGDGFIDAEELKKVLCAMGFTVASGI 180

[Close]

BLAST of Tan0012178 vs. ExPASy TrEMBL
Match: A0A6J1ECX5 (probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC111431403 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 1.3e-65
Identity = 134/171 (78.36%), Postives = 148/171 (86.55%), Query Frame = 0


Query: 65  ILCAAFLFYLWNSWVAHRNIIKRLDAAE-AACTPSVRRNAHEGHYYTELVDVPVNDKHEV 124
           +LCAAF FYLWNSWVAHRNIIKR +A++ A CTPS RRNAH GH YT +V +PVND HEV
Sbjct: 1   MLCAAFHFYLWNSWVAHRNIIKRFEASDGATCTPSARRNAHVGHCYTGIVGMPVNDAHEV 60

Query: 125 KLTIEDVKTMMEALDRGLNNNENNNLGLNAEIEGLFEEELSLDEVEEAFDLFDENGDGFI 184
           KLTIEDVKTMMEAL    NN E NNL L AEIEG+FEEELS+ EV+EAFDLFDENGDGFI
Sbjct: 61  KLTIEDVKTMMEALH---NNMETNNLELKAEIEGVFEEELSVGEVKEAFDLFDENGDGFI 120

Query: 185 DAEDLKKVLCALGFTVASEVDQCRRMIRVFDDDGDGRLDFKEFAKLVEQSF 235
           DAEDLKKVL A+GFTVASEVD+CRRMI  +D +GDGRLDF+EFAKLVEQSF
Sbjct: 121 DAEDLKKVLDAMGFTVASEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSF 168

[Close]

BLAST of Tan0012178 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.3e-19
Identity = 51/118 (43.22%), Postives = 76/118 (64.41%), Query Frame = 0


Query: 118 NDKHEVKLTIEDVKTMMEALDRGLNNNENNNLGLNAEIEGLFEE-ELSLDEVEEAFDLFD 177
           +D  ++ ++ E+ + +M +L    N+++        E+  LFEE E SL+EV++AFD+FD
Sbjct: 76  DDDDDIDISREEAEMVMRSLGLFYNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVFD 135

Query: 178 ENGDGFIDAEDLKKVLCALGFTVASEVDQCRRMIRVFDDDGDGRLDFKEFAKLVEQSF 235
           EN DGFIDA +L++VL  LGF   S +D C  MIR  D + DG++DF EF K +E SF
Sbjct: 136 ENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETSF 193

[Close]

BLAST of Tan0012178 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.3e-19
Identity = 59/133 (44.36%), Postives = 81/133 (60.90%), Query Frame = 0


Query: 108 YYTELVDVPVNDKHEVKLTIEDVKTMMEALDRGLNNNENNNLGL-----NAEIEGLFEE- 167
           + T +      D  +  L  EDV  +M++L  GL+ ++ N  GL     + E+  LFEE 
Sbjct: 74  FQTSIKHEEYRDDDDDGLCREDVGMVMKSL--GLSTDQENE-GLQKQYSSKEVSNLFEEK 133

Query: 168 ELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEVDQCRRMIRVFDDDGDGRL 227
           E SL+EV++AFD+FDEN DGFID  DL++VL  LG    S ++ CRRMIR FD   DGR+
Sbjct: 134 EPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRI 193

Query: 228 DFKEFAKLVEQSF 235
           DF  F K +E +F
Sbjct: 194 DFYGFVKFMENNF 203

[Close]

BLAST of Tan0012178 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 75.5 bits (184), Expect = 6.4e-14
Identity = 32/72 (44.44%), Postives = 54/72 (75.00%), Query Frame = 0


Query: 160 EEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEVDQCRRMIRVFDDDGDG 219
           EE+   ++++EAF++FD+NGDGFI  ++LK VL +LG      +D C++MI+  D DGDG
Sbjct: 72  EEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDG 131

Query: 220 RLDFKEFAKLVE 232
           R+++KEF ++++
Sbjct: 132 RVNYKEFRQMMK 143

[Close]

BLAST of Tan0012178 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 73.9 bits (180), Expect = 1.9e-13
Identity = 64/207 (30.92%), Postives = 107/207 (51.69%), Query Frame = 0


Query: 34  DDNPISGFEILVLIFLNFILERFFSGLLYIFILCAAFLFYLW--NSWVAHRNIIKRLDAA 93
           D + +S   +L ++   FIL      ++  F     F F+L+  N+++   +I       
Sbjct: 3   DSSLLSPISLLTIVIFLFILNLMM--IIQDFSSSFPFRFHLFFSNAYILFTSI------- 62

Query: 94  EAACTPSVRRNAHEGHYYTELVDVPVNDKHEVKLTIEDVKTMM---EALDRGL-NNNENN 153
                    RN  +      +  V V ++ ++K ++E+VK ++   EAL   L    E  
Sbjct: 63  ---------RNNKQNTELPIIKKVVVPNRADIKTSVEEVKAIIDDSEALYECLIEEGEEY 122

Query: 154 NLGLNAEIEGLFEEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEVDQCR 213
            L  N        E +  + V+EAF LFDEN DGFID  +LK VL  LG+   +++ +CR
Sbjct: 123 LLEKN--------EMMGKEIVKEAFRLFDENQDGFIDENELKHVLSLLGYDECTKM-ECR 182

Query: 214 RMIRVFDDDGDGRLDFKEFAKLVEQSF 235
           +M++V+D++ DG++DF EF KL+E+SF
Sbjct: 183 KMVKVYDENRDGKIDFYEFVKLIEKSF 182

[Close]

BLAST of Tan0012178 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 73.6 bits (179), Expect = 2.4e-13
Identity = 31/73 (42.47%), Postives = 54/73 (73.97%), Query Frame = 0


Query: 160 EEELSLDEVEEAFDLFDENGDGFIDAEDLKKVLCALGFTVASEVDQCRRMIRVFDDDGDG 219
           E+E+  ++++EAF++FD NGDGFI  ++LK VL +LG      +++CR+MI   D DGDG
Sbjct: 74  EDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDG 133

Query: 220 RLDFKEFAKLVEQ 233
           R+++ EF +++++
Sbjct: 134 RVNYMEFRQMMKK 146

The following BLAST results are available for this feature:

BLAST of Tan0012178 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG5	1.9e-18	43.22	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q93Z27	1.9e-18	44.36	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q2QVI1	6.9e-13	52.38	Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9LNE7	9.1e-13	44.44	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9SN89	2.6e-12	30.92	Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ...	[more]

BLAST of Tan0012178 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895011.1	9.8e-81	77.51	probable calcium-binding protein CML45 [Benincasa hispida]	[more]
XP_023001351.1	5.9e-78	75.81	probable calcium-binding protein CML47 [Cucurbita maxima]	[more]
XP_023519469.1	3.3e-76	74.42	probable calcium-binding protein CML47 [Cucurbita pepo subsp. pepo]	[more]
KAG7020054.1	2.1e-75	75.24	putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. a...	[more]
KAA0036089.1	1.8e-74	74.50	putative calcium-binding protein CML30 [Cucumis melo var. makuwa] >TYJ98897.1 pu...	[more]

BLAST of Tan0012178 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KID5	2.9e-78	75.81	probable calcium-binding protein CML47 OS=Cucurbita maxima OX=3661 GN=LOC1114955...	[more]
A0A5A7T3X0	8.6e-75	74.50	Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BEM1	5.6e-74	74.00	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103488781 P...	[more]
A0A6J1C8B5	5.6e-66	74.73	uncharacterized protein LOC111008903 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1ECX5	1.3e-65	78.36	probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]

BLAST of Tan0012178 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29000.1	1.3e-19	43.22	Calcium-binding EF-hand family protein	[more]
AT5G39670.1	1.3e-19	44.36	Calcium-binding EF-hand family protein	[more]
AT1G05990.1	6.4e-14	44.44	EF hand calcium-binding protein family	[more]
AT3G47480.1	1.9e-13	30.92	Calcium-binding EF-hand family protein	[more]
AT4G03290.1	2.4e-13	42.47	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 141..161
None	No IPR available	GENE3D	1.10.238.10		coord: 206..233 e-value: 4.4E-6 score: 28.4
None	No IPR available	GENE3D	1.10.238.10		coord: 158..205 e-value: 1.3E-11 score: 46.1
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 47..234
None	No IPR available	PANTHER	PTHR10891:SF745	CALCIUM-BINDING PROTEIN CML46-RELATED	coord: 47..234
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 167..195 e-value: 2.9E-5 score: 33.5 coord: 205..233 e-value: 1.2E-4 score: 31.4
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 167..230 e-value: 1.3E-11 score: 44.7
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 163..198 score: 15.517639
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 201..235 score: 11.947029
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 167..230 e-value: 1.06817E-15 score: 67.1877
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 214..226
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 176..188
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 124..230

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0012178.1	Tan0012178.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006464	cellular protein modification process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding