Tan0012149 (gene) Snake gourd v1

Overview
NameTan0012149
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Description(R)-mandelonitrile lyase 1-like
LocationLG08: 9662458 .. 9665116 (-)
RNA-Seq ExpressionTan0012149
SyntenyTan0012149
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCTACAAATAAAGCTTTTCAAAACCATGGACCATTCCTTGGCTACCTTTCTCTTGCTCATCCTTGTCTCTATCTTTCATTTCCAATTGCGAGTCCTCCCTTCACACACTCTTCCCAATCAAGGTAAATCTCCTCCCACTCTTAAAGAAAAAAAAAAAAAATCCATGTCGTACCTGATCTCTAATGTATTTTTTGTTGGATATGGTTTTGAAATTAATTCTCTGTAATAATTATTATTGTTCTAAATTTCCTGTTCCAGATGCTAGCTACATGAAGTTTGTACATGATGCCAATGACTTAACAAAGAAAGAAGAATATGATTACATAATAATAGGAGGAGGAACGGCCGGTTGCCCATTAGCTGCAACATTGTCATCAAAATTCTCTGTTCTCCTTCTTGAAAGAGGCAGTGTCCCTAACAAATATCCCTCTGTACTGAAAGAACAAGATCTATTGAACATTTTTTCTACAGAAGATGATGGCAAAAATCCCTTCAACCGGTTCATCTCTGAGGACGGTGTAGAGAACATAAGAGGACGAGTCCTTGGCGGCAGTAGCATGATCAATGCTGGCTTCTACTCGAGGGGCCATCGAGAGTTCTTTGAAACTGCAGACGTTGATTGGGACATGGAATTGGTGGAAAAGTCTTATCAATGGGTTGAACAGACTGTGGTGTCTCGACCAATTTTGAGTGCTTGGCAATCTAGTTTTAGAAGTGCGCTATTGGAAGCTGGGGTTGTACCTGATAATGGGTTTGATTTGAGACACCTTGTAGGGACTAAAATTGGAGGTTCCATCTTTGATGACAACGGTAACAGACATGGCGCTGTGGAGCTTCTCAACAAGGCTGAACCCGAAAATCTTAAAGTTGTAATCCATGCAACAGTAGAAAGAATCATCTTCTCTGGTCAGTGCATAATGAATCTAACTTAATTTCATTCCATTAATCGAATACTAAAAACCTGTTTGGTAACGTTTTCTTTTTTTTTTCCTATTTCTGTTTGGAAACATATTTTAAGAAAAAATTGTCATAAATTTTACTCAATTTTGGTGAATTAGTTGAAGTGAGAGATGGTATTACAAATCAATTTTAAATCAGATTTGAAGGATTAAATTTATCAATATTAATATTAATATTATTATTCATTTACTTTGGAGCTGAAATTGTATTAAATATTATAAATCTTTTATAATGGTTTTATAGTTCGTTTAATGCATGTTATATATTTTATGATATTTATATATATATATATAATTTTATTTCAACTAAAAAAATAATAAACAATTATCAAGCAGATTTTTTATTCTTTTTGCAAGGAAAAAAACGAGAAATAATTATTTAACATGTTTCTATATTTGGTTCTTAAAAAATAAGAAACCGAAAACAAGAAATGGGTAACTTTATCAAACAAGCCTAAATTTTCACACAATTTCTGAACTCTTCGTAGGTGTATCTGCAACTGGGGTCTCATATTCTGATTCAAAAAGTAACTTGCATACAGCATTCATTCGAAAGAAAGGAGAGATTATTTTAAGTGCTGGAGCCATTGGAAGTCCTCAACTTCTTCTTCTAAGTGGGATTGGTCCAAAATCTCATCTTTCATCCTTAAAACTACCTGTCGTCCTTCACCAACCACATGTCGGCAATTCCATGTCCGACAATCCCCGTTACGGAGCCAACATTATGCTTCCATTCCAAATAGTTCCTACATCTGCAAAAGTCGTCGGTATTTTAGAAAATAATATCTATTTCCAAACAATCGGCAGTATTTTACCATTTTCACTTCCACCGTCCTTTAGTCTTCTTCCTCCTCAATCTGATTCAGTAACTATGACCCTAGCCACCATCTTTGGAAAATTCTCTGAGGTCCTTTCGGTGGGATCGTTACGGCTTAATTCTTCCACTGATGTGAAGAAGAGTCCCATTGTCCAATTCAATTATTATTCTCATCCTGATGATGTTGCTAAATGTGTTAGAGGACTTCGAAAATTGGGAGAATTGCTTCAAACCCAAACAATGGAAAAGATTAAAACGCAAGATTTAGAGGGTAAGAAAACATTTCAATTTCTTGGACCCATGTTTCCGAAAAATATGTCGGATGATAATTCGGTTGGAGAATTCTGTAAGAAAACAGTGACAACATATTGGCATTACCATGGAGGATGTTTGGTTGGAAAAGTTGTCGACAGTAATTACAGAGTCATTGGAATTAAAAATCTTCGTGTCGTTGATGGCTCCACTTTCTCCGATTCACCTGGAACCAATCCTATGGCCACCCTCATGATGCTCGGCCGGTCAGTTTTTATATATATAAACAGCAAATATGGAAATTTATGAAATAAAATGTGGAAATCCTTTTTTTTTTTATAATTTAATTAACAACAGTATTAATAATTTCTTGAAAAAAAAATTACTAGAGGTTCTACGCCAAGAGATTATTTTATTGTCTTTTTATTTCCATAAACTAACAATTCCACCAAATTATTTTACAGGTATGTTGGGGTTAAGATATTGCAAGAAAGATCAAGTTAAGGAGTGTAAACTATCCAAGACCATTTCATAATGTTCATGTTTGTCCAATCCATGTATCCTATTTCCAATTTTAATTTGAATAATATTATGGTCGTTGGTCTTTACTTTTCTTTTCTCCCATCAAC

mRNA sequence

TCTCTACAAATAAAGCTTTTCAAAACCATGGACCATTCCTTGGCTACCTTTCTCTTGCTCATCCTTGTCTCTATCTTTCATTTCCAATTGCGAGTCCTCCCTTCACACACTCTTCCCAATCAAGATGCTAGCTACATGAAGTTTGTACATGATGCCAATGACTTAACAAAGAAAGAAGAATATGATTACATAATAATAGGAGGAGGAACGGCCGGTTGCCCATTAGCTGCAACATTGTCATCAAAATTCTCTGTTCTCCTTCTTGAAAGAGGCAGTGTCCCTAACAAATATCCCTCTGTACTGAAAGAACAAGATCTATTGAACATTTTTTCTACAGAAGATGATGGCAAAAATCCCTTCAACCGGTTCATCTCTGAGGACGGTGTAGAGAACATAAGAGGACGAGTCCTTGGCGGCAGTAGCATGATCAATGCTGGCTTCTACTCGAGGGGCCATCGAGAGTTCTTTGAAACTGCAGACGTTGATTGGGACATGGAATTGGTGGAAAAGTCTTATCAATGGGTTGAACAGACTGTGGTGTCTCGACCAATTTTGAGTGCTTGGCAATCTAGTTTTAGAAGTGCGCTATTGGAAGCTGGGGTTGTACCTGATAATGGGTTTGATTTGAGACACCTTGTAGGGACTAAAATTGGAGGTTCCATCTTTGATGACAACGGTAACAGACATGGCGCTGTGGAGCTTCTCAACAAGGCTGAACCCGAAAATCTTAAAGTTGTAATCCATGCAACAGTAGAAAGAATCATCTTCTCTGGTGTATCTGCAACTGGGGTCTCATATTCTGATTCAAAAAGTAACTTGCATACAGCATTCATTCGAAAGAAAGGAGAGATTATTTTAAGTGCTGGAGCCATTGGAAGTCCTCAACTTCTTCTTCTAAGTGGGATTGGTCCAAAATCTCATCTTTCATCCTTAAAACTACCTGTCGTCCTTCACCAACCACATGTCGGCAATTCCATGTCCGACAATCCCCGTTACGGAGCCAACATTATGCTTCCATTCCAAATAGTTCCTACATCTGCAAAAGTCGTCGGTATTTTAGAAAATAATATCTATTTCCAAACAATCGGCAGTATTTTACCATTTTCACTTCCACCGTCCTTTAGTCTTCTTCCTCCTCAATCTGATTCAGTAACTATGACCCTAGCCACCATCTTTGGAAAATTCTCTGAGGTCCTTTCGGTGGGATCGTTACGGCTTAATTCTTCCACTGATGTGAAGAAGAGTCCCATTGTCCAATTCAATTATTATTCTCATCCTGATGATGTTGCTAAATGTGTTAGAGGACTTCGAAAATTGGGAGAATTGCTTCAAACCCAAACAATGGAAAAGATTAAAACGCAAGATTTAGAGGGTAAGAAAACATTTCAATTTCTTGGACCCATGTTTCCGAAAAATATGTCGGATGATAATTCGGTTGGAGAATTCTGTAAGAAAACAGTGACAACATATTGGCATTACCATGGAGGATGTTTGGTTGGAAAAGTTGTCGACAGTAATTACAGAGTCATTGGAATTAAAAATCTTCGTGTCGTTGATGGCTCCACTTTCTCCGATTCACCTGGAACCAATCCTATGGCCACCCTCATGATGCTCGGCCGGTATGTTGGGGTTAAGATATTGCAAGAAAGATCAAGTTAAGGAGTGTAAACTATCCAAGACCATTTCATAATGTTCATGTTTGTCCAATCCATGTATCCTATTTCCAATTTTAATTTGAATAATATTATGGTCGTTGGTCTTTACTTTTCTTTTCTCCCATCAAC

Coding sequence (CDS)

ATGGACCATTCCTTGGCTACCTTTCTCTTGCTCATCCTTGTCTCTATCTTTCATTTCCAATTGCGAGTCCTCCCTTCACACACTCTTCCCAATCAAGATGCTAGCTACATGAAGTTTGTACATGATGCCAATGACTTAACAAAGAAAGAAGAATATGATTACATAATAATAGGAGGAGGAACGGCCGGTTGCCCATTAGCTGCAACATTGTCATCAAAATTCTCTGTTCTCCTTCTTGAAAGAGGCAGTGTCCCTAACAAATATCCCTCTGTACTGAAAGAACAAGATCTATTGAACATTTTTTCTACAGAAGATGATGGCAAAAATCCCTTCAACCGGTTCATCTCTGAGGACGGTGTAGAGAACATAAGAGGACGAGTCCTTGGCGGCAGTAGCATGATCAATGCTGGCTTCTACTCGAGGGGCCATCGAGAGTTCTTTGAAACTGCAGACGTTGATTGGGACATGGAATTGGTGGAAAAGTCTTATCAATGGGTTGAACAGACTGTGGTGTCTCGACCAATTTTGAGTGCTTGGCAATCTAGTTTTAGAAGTGCGCTATTGGAAGCTGGGGTTGTACCTGATAATGGGTTTGATTTGAGACACCTTGTAGGGACTAAAATTGGAGGTTCCATCTTTGATGACAACGGTAACAGACATGGCGCTGTGGAGCTTCTCAACAAGGCTGAACCCGAAAATCTTAAAGTTGTAATCCATGCAACAGTAGAAAGAATCATCTTCTCTGGTGTATCTGCAACTGGGGTCTCATATTCTGATTCAAAAAGTAACTTGCATACAGCATTCATTCGAAAGAAAGGAGAGATTATTTTAAGTGCTGGAGCCATTGGAAGTCCTCAACTTCTTCTTCTAAGTGGGATTGGTCCAAAATCTCATCTTTCATCCTTAAAACTACCTGTCGTCCTTCACCAACCACATGTCGGCAATTCCATGTCCGACAATCCCCGTTACGGAGCCAACATTATGCTTCCATTCCAAATAGTTCCTACATCTGCAAAAGTCGTCGGTATTTTAGAAAATAATATCTATTTCCAAACAATCGGCAGTATTTTACCATTTTCACTTCCACCGTCCTTTAGTCTTCTTCCTCCTCAATCTGATTCAGTAACTATGACCCTAGCCACCATCTTTGGAAAATTCTCTGAGGTCCTTTCGGTGGGATCGTTACGGCTTAATTCTTCCACTGATGTGAAGAAGAGTCCCATTGTCCAATTCAATTATTATTCTCATCCTGATGATGTTGCTAAATGTGTTAGAGGACTTCGAAAATTGGGAGAATTGCTTCAAACCCAAACAATGGAAAAGATTAAAACGCAAGATTTAGAGGGTAAGAAAACATTTCAATTTCTTGGACCCATGTTTCCGAAAAATATGTCGGATGATAATTCGGTTGGAGAATTCTGTAAGAAAACAGTGACAACATATTGGCATTACCATGGAGGATGTTTGGTTGGAAAAGTTGTCGACAGTAATTACAGAGTCATTGGAATTAAAAATCTTCGTGTCGTTGATGGCTCCACTTTCTCCGATTCACCTGGAACCAATCCTATGGCCACCCTCATGATGCTCGGCCGGTATGTTGGGGTTAAGATATTGCAAGAAAGATCAAGTTAA

Protein sequence

MDHSLATFLLLILVSIFHFQLRVLPSHTLPNQDASYMKFVHDANDLTKKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVENIRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILSAWQSSFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVVIHATVERIIFSGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTIGSILPFSLPPSFSLLPPQSDSVTMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHPDDVAKCVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEFCKKTVTTYWHYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQERSS
Homology
[Close]
BLAST of Tan0012149 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 2.8e-158
Identity = 288/550 (52.36%), Postives = 380/550 (69.09%), Query Frame = 0 

Query: 1   MDHSLATFLLLILVSIFHFQLRVLPSHTL---PNQDASYMKFVHDANDLTKKEEYDYIII 60
           M+ S  + +LL+L  +F   L+    H+L    N D SY++F +DA DL  +  YDY+I+
Sbjct: 1   MEKSTMSAILLVL-HLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIV 60

Query: 61  GGGTAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISE 120
           GGGT+GCPLAATLS K+ VL+LERGS+P  YP+VL     +     EDDGK P  RF+SE
Sbjct: 61  GGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSE 120

Query: 121 DGVENIRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILS 180
           DG++N+RGRVLGG+SMINAG Y+R +   +  + VDWDM+LV K+Y+WVE T+V +P   
Sbjct: 121 DGIDNVRGRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQ 180

Query: 181 AWQSSFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVV 240
            WQS   +A LEAGV P++GF L H  GT+I GS FD+ G RH A ELLNK    NL+V 
Sbjct: 181 PWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVG 240

Query: 241 IHATVERIIFS---GVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGIG 300
           +HA+VE+IIFS   G++ATGV Y DS    H AF+R KGE+I+SAG IG+PQLLLLSG+G
Sbjct: 241 VHASVEKIIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVG 300

Query: 301 PKSHLSSLKLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTIG 360
           P+S+LSSL +PVVL  P+VG  + DNPR   NI+ P  I PT   V+GI  +N ++Q   
Sbjct: 301 PESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSF 360

Query: 361 SILPFSLPPSFSLLPPQSDSV-TMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNY 420
           S LPF+ PP FS  P  S  +   T A    K +  LS GSL L SS++V+ SP V+FNY
Sbjct: 361 SSLPFTTPP-FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNY 420

Query: 421 YSHPDDVAKCVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEF 480
           YS+P D++ CV G++K+GELL T  ++  K +DL G + F  LG   PK+ +DD +   F
Sbjct: 421 YSNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETF 480

Query: 481 CKKTVTTYWHYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYV 540
           C+++V +YWHYHGGCLVGKV+D ++RV GI  LRVVDGSTF  +P ++P    +MLGRYV
Sbjct: 481 CRESVASYWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYV 540

Query: 541 GVKILQERSS 544
           G+KILQERS+
Sbjct: 541 GIKILQERSA 546

[Close]
BLAST of Tan0012149 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 2.6e-156
Identity = 280/545 (51.38%), Postives = 377/545 (69.17%), Query Frame = 0 

Query: 4   SLATFLLLILVSIFHFQLRVLPS-HTLPNQDASYMKFVHDANDLTKKEEYDYIIIGGGTA 63
           +++  LL++ + + H Q   + S  T  + D SY+ F +DA DL  +  YDY+I+GGGT+
Sbjct: 5   TMSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTS 64

Query: 64  GCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVEN 123
           GCPLAATLS K+ VL+LERGS+P  YP+VL     +     EDDGK P  RF+SEDG++N
Sbjct: 65  GCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDN 124

Query: 124 IRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILSAWQSS 183
           +RGRVLGG+S+INAG Y+R +   +  + VDWDM+LV ++Y+WVE T+V +P   +WQS 
Sbjct: 125 VRGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQSV 184

Query: 184 FRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVVIHATV 243
            ++A LEAGV P++GF L H  GT+I GS FD+ G RH A ELLNK    NL+V +HA+V
Sbjct: 185 TKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASV 244

Query: 244 ERIIFS---GVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHL 303
           E+IIFS   G++ATGV Y DS    H AF+R KGE+I+SAG IG+PQLLLLSG+GP+S+L
Sbjct: 245 EKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYL 304

Query: 304 SSLKLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTIGSILPF 363
           SSL +PVVL  P+VG  + DNPR   NI+ P  I PT   V+GI  +N ++Q   S LPF
Sbjct: 305 SSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLPF 364

Query: 364 SLPPSFSLLPPQSDSV-TMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHPD 423
           + PP F   P  S  +   T A    K +  LS GSL L SS++V+ SP V+FNYYS+  
Sbjct: 365 TTPP-FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLT 424

Query: 424 DVAKCVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEFCKKTV 483
           D++ CV G++K+GELL T  ++  K +DL G + F  LG   PK+ +DD +   FC+++V
Sbjct: 425 DLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESV 484

Query: 484 TTYWHYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKIL 543
            +YWHYHGGCLVGKV+D ++RV GI  LRVVDGSTF  +P ++P    +MLGRYVG+KIL
Sbjct: 485 ASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKIL 544

[Close]
BLAST of Tan0012149 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 2.1e-153
Identity = 274/546 (50.18%), Postives = 372/546 (68.13%), Query Frame = 0 

Query: 4   SLATFLLLILVSIFHFQLRVLPS-HTLPNQDASYMKFVHDANDLTKKEEYDYIIIGGGTA 63
           +++  LL++ + + H Q   + S     + D SY+ FV+DA D   +  YDYII+GGGTA
Sbjct: 5   TMSAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTA 64

Query: 64  GCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVEN 123
           GCPLAATLS+ +SVL+LERGS+P +YP++L     +     EDDGK P  RF+SEDG++N
Sbjct: 65  GCPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDN 124

Query: 124 IRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILSAWQSS 183
           +RGRVLGG+SMINAG Y R +  FF    ++WDM+LV ++Y+WVE T+V  P    WQ+ 
Sbjct: 125 VRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTV 184

Query: 184 FRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVVIHATV 243
             +A LEAG++P+NGF + HL GT++ GS FD+NG RH + ELLNK +P NL+V + A V
Sbjct: 185 IGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAV 244

Query: 244 ERIIF----SGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSH 303
           E+IIF    SGV+A GV Y+DS    H AF+R +GE+ILSAG IGSPQLLLLSG+GP+S+
Sbjct: 245 EKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESY 304

Query: 304 LSSLKLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTIGSILP 363
           L+SL + VV   P+VG  + DNPR   NI+ P  I  ++  V+GI  +  ++Q   S LP
Sbjct: 305 LTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSISSLP 364

Query: 364 FSLPPSFSLLPPQSDSV-TMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHP 423
           F  PP FS  P  S  +   T A I  K    LS G++ LNSS+DV+  P V+FNYYS+ 
Sbjct: 365 FDTPP-FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNL 424

Query: 424 DDVAKCVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEFCKKT 483
            D++ CV G++KLGE+L T  +E  K +DL G   F  LG   P+N +DD +   FC+++
Sbjct: 425 TDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRES 484

Query: 484 VTTYWHYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKI 543
           V +YWHYHGGCLVGKV+D  +RV GI  LRVVDGSTF  +P ++P    +MLGRY+G++I
Sbjct: 485 VASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGIQI 544

[Close]
BLAST of Tan0012149 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 4.1e-149
Identity = 270/549 (49.18%), Postives = 366/549 (66.67%), Query Frame = 0 

Query: 1   MDHSLATFLLLILVSIFHFQLRVLPSHTLPN---QDASYMKFVHDANDLTKKEEYDYIII 60
           M  S  + +L++ + +F   L+     +L N    D SY++FV+DAND   +  YDYII+
Sbjct: 1   MVKSTMSAILVLALHLFVLHLQYSEVQSLANTSAHDFSYLEFVYDANDTELEGTYDYIIV 60

Query: 61  GGGTAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISE 120
           GGGTAGCPLAATLS+ +SVL+LERG++P +YP++L     +     EDDG+ P  RF+S 
Sbjct: 61  GGGTAGCPLAATLSANYSVLVLERGTLPTEYPNLLTSDGFIYNLQQEDDGQTPVERFVSG 120

Query: 121 DGVENIRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILS 180
           DG++N+RGRVLGG+SMINAG Y R +  FF    ++WDM+LV K+Y WVE T+V +P   
Sbjct: 121 DGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNKTYDWVEDTIVFKPDFQ 180

Query: 181 AWQSSFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVV 240
            WQ+   +A LE G++PDNGF L HL GT++ GS FD+NG RH + ELLNK +P NL+V 
Sbjct: 181 FWQNLTGTAFLEVGILPDNGFSLDHLEGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVA 240

Query: 241 IHATVERIIF----SGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGI 300
           +HA VE+IIF    SGV+A GV Y+DS    H AF+R  GE+ILSAG IGSPQLLLLSG+
Sbjct: 241 VHAAVEKIIFSSDSSGVTAIGVIYTDSNGTTHQAFVRGDGEVILSAGPIGSPQLLLLSGV 300

Query: 301 GPKSHLSSLKLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTI 360
           G +S+L+SL + VV   P+VG  + DNPR   NI+ P  I  ++  V+GI  +  ++Q  
Sbjct: 301 GLESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCS 360

Query: 361 GSILPFSLPPSFSLLP-PQSDSVTMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFN 420
            S LPFS  P F   P P       T A I  K    LS G++ L S++DV+ +P V FN
Sbjct: 361 ISSLPFSTAP-FGFFPNPTYPLPNTTFAHIVNKVPGPLSHGTVLLQSTSDVRVAPNVTFN 420

Query: 421 YYSHPDDVAKCVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGE 480
           YYS+  D+A CV G++K+GE L +  ++  K +DL G + F  LG   P+N +DD +   
Sbjct: 421 YYSNTTDLAHCVSGMKKIGEFLSSDALKPYKVEDLPGIEGFDILGIPLPENQTDDAAFET 480

Query: 481 FCKKTVTTYWHYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRY 540
           FC++ V +YWHYHGGCLVG+V+D ++RV GI  LRVVDGSTF  +P ++P    +MLGRY
Sbjct: 481 FCREAVASYWHYHGGCLVGEVLDDDFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRY 540

Query: 541 VGVKILQER 542
           +G KILQER
Sbjct: 541 MGTKILQER 546

[Close]
BLAST of Tan0012149 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 1.0e-147
Identity = 265/553 (47.92%), Postives = 372/553 (67.27%), Query Frame = 0 

Query: 1   MDHSL--ATFLLLILVSIFHFQLRVLPSHTLP--NQDASYMKFVHDANDLTKKEEYDYII 60
           +D SL     ++L+L+ + H       S+  P  N+   +M+F+ +A D   ++ YDYII
Sbjct: 5   IDSSLLYTALVVLLLLGVVH------RSNARPRVNRPPGFMRFISNATDFASEDYYDYII 64

Query: 61  IGGGTAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFIS 120
           +GGGTAGCPLAATLS  F VLLLERG VP   P+V+     L   +  ++  +P   FIS
Sbjct: 65  VGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFIS 124

Query: 121 EDGVENIRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPIL 180
           E+GV N RGRVLGGSS INAGFYSR  ++FFE + + WD+  V +SY+WVE+ +V RP L
Sbjct: 125 EEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQL 184

Query: 181 SAWQSSFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKV 240
             WQ++ R ALLE GV P NGF L H VGTKIGGS FD  G RH + +LL  A   N++V
Sbjct: 185 RTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRV 244

Query: 241 VIHATVERIIF--------SGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLL 300
            ++ATVER++         S VSA GV Y D     H A IR +GE+ILSAGA+GSPQLL
Sbjct: 245 AVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLL 304

Query: 301 LLSGIGPKSHLSSLKLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNI 360
            LSGIGP+S+LS+  +PV L QPHVG+ + DNPR G +I+ P  +  +  +VVG+ E+  
Sbjct: 305 FLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGA 364

Query: 361 YFQTIGSILPFSLPPSFSLLPPQSDSVTMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPI 420
           + +   +++PF+ P     +   +  + + + TI  K    +S+G LRL +STDV+ +P+
Sbjct: 365 FLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPV 424

Query: 421 VQFNYYSHPDDVAKCVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDN 480
           V+FNY+S P D+ +CV G RK+GE+L+++ M+    ++  G + F+F+G   P + S+D 
Sbjct: 425 VRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDL 484

Query: 481 SVGEFCKKTVTTYWHYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMM 540
            + +FC++TV+T WHYHGG +VGKVVDS+ +VIG+ +LR+VDGSTF+ SPGTNP ATLMM
Sbjct: 485 VMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMM 544

Query: 541 LGRYVGVKILQER 542
           LGRY+G+K+L+ER
Sbjct: 545 LGRYMGLKMLRER 550

[Close]
BLAST of Tan0012149 vs. NCBI nr
Match: XP_022153314.1 ((R)-mandelonitrile lyase 1-like [Momordica charantia])

HSP 1 Score: 879.4 bits (2271), Expect = 1.6e-251
Identity = 431/539 (79.96%), Postives = 483/539 (89.61%), Query Frame = 0 

Query: 3   HSLATFLLLILVSIFHFQLRVLPSHTLPNQDASYMKFVHDANDLTKKEEYDYIIIGGGTA 62
           +S+A FLLL +VSIFHFQL+VL SHT+PNQD SYMKFV DA DL +KEEYDYII+GGGTA
Sbjct: 4   YSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGGGTA 63

Query: 63  GCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVEN 122
           GCPLA TLSSKFSVLLLERGS PNK+PSVL E+ LLN F+ EDD KNPF RFISEDGVEN
Sbjct: 64  GCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDGVEN 123

Query: 123 IRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILSAWQSS 182
           +RGRVLGGSSMINAGFYSRGHRE+FE A V+WDMELVEK+YQWVE+TVVSRP LSA Q +
Sbjct: 124 LRGRVLGGSSMINAGFYSRGHREYFEKAGVEWDMELVEKAYQWVEETVVSRPSLSALQPT 183

Query: 183 FRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVVIHATV 242
           FRSALLE+GV+PDNGF LRHLVGT+IGGSIFD  GNRHGAVELLNKA P NLKV I ATV
Sbjct: 184 FRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNRHGAVELLNKANPTNLKVAIRATV 243

Query: 243 ERIIFSGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSL 302
           ERIIFSG+SA+GVSYSDS+  LHTAFIR KGEIILSAGAIGSPQLLLLSGIGPKS LSSL
Sbjct: 244 ERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQLSSL 303

Query: 303 KLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTIGSILPFSLP 362
           KLPVVLHQP+VG  MSDNPR+G +I+LPF  VPTSAKVVG LENNI+ QT+ S LPFS+P
Sbjct: 304 KLPVVLHQPYVGEFMSDNPRFGPSIVLPFPFVPTSAKVVGTLENNIHLQTVASPLPFSIP 363

Query: 363 PSFSLLPPQSDSVTMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHPDDVAK 422
           PSFSLLPPQS+SV ++L    GKFSEVLS GSLRLNSSTDVKKSPIV+FNYYSHPDD+A+
Sbjct: 364 PSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDDLAR 423

Query: 423 CVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEFCKKTVTTYW 482
           CV G+RK+G+L++T TMEKIKT+D EGKK FQF+GP  P+N+SDD++VGEFC K++TTYW
Sbjct: 424 CVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSLTTYW 483

Query: 483 HYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQER 542
           HYHGGCLVGKVVD +YRVIGI NLRVVDGSTF DSPGTNPMATLMMLGRYVG+K+LQ+R
Sbjct: 484 HYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFIDSPGTNPMATLMMLGRYVGLKVLQQR 542

[Close]
BLAST of Tan0012149 vs. NCBI nr
Match: XP_022153315.1 ((R)-mandelonitrile lyase 1-like [Momordica charantia])

HSP 1 Score: 873.6 bits (2256), Expect = 8.7e-250
Identity = 428/539 (79.41%), Postives = 481/539 (89.24%), Query Frame = 0 

Query: 3   HSLATFLLLILVSIFHFQLRVLPSHTLPNQDASYMKFVHDANDLTKKEEYDYIIIGGGTA 62
           +S+A FLLL +VSIFHFQL+VL SHT+PNQD SYMKFV DA DL +KEEYDYII+GGGTA
Sbjct: 4   YSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGGGTA 63

Query: 63  GCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVEN 122
           GCPLA TLSSKFSVLLLERGS PNK+PSVL E+ LLN F+ EDD KNPF RFISEDGVEN
Sbjct: 64  GCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDGVEN 123

Query: 123 IRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILSAWQSS 182
           +RGRVLGGSSMINAGFYSRGH+EFFE A V+WDMELVEK+YQWVE+TVVSRP LSA Q +
Sbjct: 124 LRGRVLGGSSMINAGFYSRGHQEFFEKAGVEWDMELVEKAYQWVEETVVSRPSLSALQPT 183

Query: 183 FRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVVIHATV 242
           FRSALLE+GV+PDNGF LRHLVGT+IGGSIFD  GN HGAVELLNKA P N+KV I  TV
Sbjct: 184 FRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNPHGAVELLNKANPTNIKVAIRTTV 243

Query: 243 ERIIFSGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSL 302
           ERIIFSG+SA+GVSYSDS+  LHTAFIR KGEIILSAGAIGSPQLLLLSGIGPKS LSSL
Sbjct: 244 ERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQLSSL 303

Query: 303 KLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTIGSILPFSLP 362
           KLPVVLHQP+VG  MSDNPR+G +I+LPF  VPTSAKVVG LENNI+ QT+ S LPFS+P
Sbjct: 304 KLPVVLHQPYVGEFMSDNPRFGPSIVLPFPXVPTSAKVVGTLENNIHLQTVASPLPFSIP 363

Query: 363 PSFSLLPPQSDSVTMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHPDDVAK 422
           PSFSLLPPQS+SV ++L    GKFSEVLS GSLRLNSSTDVKKSPIV+FNYYSHPDD+A+
Sbjct: 364 PSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDDLAR 423

Query: 423 CVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEFCKKTVTTYW 482
           CV G+RK+G+L++T TMEKIKT+D EGKK FQF+GP  P+N+SDD++VGEFC K++TTYW
Sbjct: 424 CVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSLTTYW 483

Query: 483 HYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQER 542
           HYHGGCLVGKVVD +YRVIGI NLRVVDGSTF DSPGTNPMATLMMLGRYVG+K+LQ+R
Sbjct: 484 HYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFIDSPGTNPMATLMMLGRYVGLKVLQQR 542

[Close]
BLAST of Tan0012149 vs. NCBI nr
Match: XP_038889314.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 861.3 bits (2224), Expect = 4.5e-246
Identity = 425/543 (78.27%), Postives = 480/543 (88.40%), Query Frame = 0 

Query: 1   MDHSLATFLLLILVSIFHFQLRVLPSHTLPNQDASYMKFVHDANDLTKKEEYDYIIIGGG 60
           M+HS AT L+L L+ I   QL VL  + +P +D  YMKFVH A+DL  KEEYDYIIIGGG
Sbjct: 1   MEHSKATILILSLM-ISISQLGVLTYNAIPIKDVRYMKFVHKASDLPAKEEYDYIIIGGG 60

Query: 61  TAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGV 120
           TAGCPLAATLSSKFSVL+LERGS PNKYPSVL EQ LLN+F+  DDGKNPF RFISEDGV
Sbjct: 61  TAGCPLAATLSSKFSVLVLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFISEDGV 120

Query: 121 ENIRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILSAWQ 180
           ENIR RVLGG SM+NAGFYSRGH++FFETA VDWDMELVEK+YQWVE+TV SRPIL+AWQ
Sbjct: 121 ENIRARVLGGGSMVNAGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVASRPILNAWQ 180

Query: 181 SSFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVVIHA 240
           S+FRSALLEAGVVPDNGFDLRHLVGTK GGSIFDD GNRHGAVELLNKAEP N+KV I A
Sbjct: 181 SAFRSALLEAGVVPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPTNIKVAIEA 240

Query: 241 TVERIIFSGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLS 300
           TV++I+FSG+SA GV YSDSK  LH AFIRKKGEII+SAGAIGSPQLLLLSG+GPKSHLS
Sbjct: 241 TVQKILFSGLSARGVLYSDSKGKLHRAFIRKKGEIIVSAGAIGSPQLLLLSGVGPKSHLS 300

Query: 301 SLKLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTIGSILPFS 360
           SLKLPVVLHQP+VG  MSDNPR+G NI+LPFQ+VP+S KVVGIL++NIY Q+  S  PF 
Sbjct: 301 SLKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPFF 360

Query: 361 LPPSFSLLPPQSDSVTMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHPDDV 420
            PPSFSLLPPQ +S+  +LA   GKFSEV S GSLRLNSST+VKKSPIV+FNYYSHPDD+
Sbjct: 361 APPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDL 420

Query: 421 AKCVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEFCKKTVTT 480
           A+CV+G+RK+G+LL+TQTMEKIKT DLEG K FQFLG   P+N+S+D+SVGE+C+KTVTT
Sbjct: 421 ARCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVGEYCQKTVTT 480

Query: 481 YWHYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQE 540
           YWHYHGGCLVGKVVD NY+VIGIKNLRVVDGSTFS+SPGTNPMATLMMLGRYVG+K+LQ+
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLQQ 540

Query: 541 RSS 544
           RSS
Sbjct: 541 RSS 542

[Close]
BLAST of Tan0012149 vs. NCBI nr
Match: XP_022153313.1 ((R)-mandelonitrile lyase 1-like [Momordica charantia])

HSP 1 Score: 858.2 bits (2216), Expect = 3.8e-245
Identity = 417/539 (77.37%), Postives = 477/539 (88.50%), Query Frame = 0 

Query: 3   HSLATFLLLILVSIFHFQLRVLPSHTLPNQDASYMKFVHDANDLTKKEEYDYIIIGGGTA 62
           +S+ TFLLL ++SIFHFQL VL SHT+ NQD SYMKFVHDA DL +  EYDYII+GGG A
Sbjct: 4   NSMDTFLLLFIISIFHFQLGVLSSHTISNQDVSYMKFVHDAKDLPETGEYDYIIVGGGAA 63

Query: 63  GCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVEN 122
           GCPLA TLSSKFS+L+LERGS PNK+PSVL EQ     F+ EDDGKNPF RF+SEDGVEN
Sbjct: 64  GCPLATTLSSKFSILILERGSEPNKFPSVLTEQGRSKAFTAEDDGKNPFQRFVSEDGVEN 123

Query: 123 IRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILSAWQSS 182
           +RGRVLGG +MIN GFYSRGHREFF+TA V+WDMELVEK+YQWVE+TVVSRP LSAWQS+
Sbjct: 124 LRGRVLGGGTMINGGFYSRGHREFFDTAGVEWDMELVEKAYQWVEETVVSRPNLSAWQSA 183

Query: 183 FRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVVIHATV 242
           FRSALLE+GV+PDNGFDLRHLVGT+IGGS FD  GNRHGAVELLNKA P NLKVVI ATV
Sbjct: 184 FRSALLESGVLPDNGFDLRHLVGTRIGGSTFDGKGNRHGAVELLNKANPANLKVVIRATV 243

Query: 243 ERIIFSGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSL 302
           ERIIFSG+SA+GVSYSDS+  LHTAFIRKKGEI LSAGAIGSPQLLLLSG+GPKS LSSL
Sbjct: 244 ERIIFSGLSASGVSYSDSEGKLHTAFIRKKGEIFLSAGAIGSPQLLLLSGVGPKSDLSSL 303

Query: 303 KLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTIGSILPFSLP 362
           KLPVVLHQPHVG  MSDNPR+  NI+LPF IV T+ KVVG L+NNI+FQ+      FS+P
Sbjct: 304 KLPVVLHQPHVGQFMSDNPRFVTNIVLPFPIVSTTGKVVGTLKNNIHFQSFAGSSLFSVP 363

Query: 363 PSFSLLPPQSDSVTMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHPDDVAK 422
           PSFSLLPP+S+SV M+LAT+ GKFS+V S+GSLRLNSSTDVKKSPIV+FNYYSHPDD+A+
Sbjct: 364 PSFSLLPPRSNSVNMSLATVVGKFSKVDSMGSLRLNSSTDVKKSPIVRFNYYSHPDDLAR 423

Query: 423 CVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEFCKKTVTTYW 482
           CV G+RK+G+L++T TMEKIKT++ EGKK FQFLGP  P+N+SD +SVGEFC KT+TTYW
Sbjct: 424 CVVGVRKIGDLIKTPTMEKIKTRNFEGKKVFQFLGPPLPENLSDYSSVGEFCHKTLTTYW 483

Query: 483 HYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQER 542
           HYHGGCLVGKVVD +YRVIGI NLRVVDGSTF++SPGTNPMATLMMLGRYVG+K++Q+R
Sbjct: 484 HYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFTESPGTNPMATLMMLGRYVGLKVMQQR 542

[Close]
BLAST of Tan0012149 vs. NCBI nr
Match: XP_038889315.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 857.8 bits (2215), Expect = 5.0e-245
Identity = 420/544 (77.21%), Postives = 480/544 (88.24%), Query Frame = 0 

Query: 1   MDHSLATFLLL-ILVSIFHFQLRVLPSHTLPNQDASYMKFVHDANDLTKKEEYDYIIIGG 60
           M+HS +T L+L +++SIFH  L +L SH +PNQD  YMKFVH A DL  KEEYDYIIIGG
Sbjct: 1   MEHSRSTILILSLMISIFH--LGLLSSHAIPNQDVGYMKFVHSATDLPAKEEYDYIIIGG 60

Query: 61  GTAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDG 120
           GTAGCPL++TLSSKFSVLLLERGS PNKYPSVL EQ LLN F+ EDDGKNPF  FISEDG
Sbjct: 61  GTAGCPLSSTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFAAEDDGKNPFQHFISEDG 120

Query: 121 VENIRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILSAW 180
           VENIRGRVLGG SM+N GFYSRGH++FFETA VDWDMELVEK+YQWVE+TVVS+PIL+AW
Sbjct: 121 VENIRGRVLGGGSMVNVGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVVSQPILNAW 180

Query: 181 QSSFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVVIH 240
           QS+FRSA L+AGVVPDNGFDLRH VGTKIGGSIFD+ GNRHGAVELLNKA P N+KV I 
Sbjct: 181 QSAFRSASLDAGVVPDNGFDLRHRVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVGIE 240

Query: 241 ATVERIIFSGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHL 300
           ATV+RI+FSG+SA GV YSDSK  LH AFIRK+GEII+SAGA+GSPQLLLLSG+GPKSHL
Sbjct: 241 ATVQRILFSGLSARGVLYSDSKGKLHRAFIRKEGEIIVSAGAVGSPQLLLLSGVGPKSHL 300

Query: 301 SSLKLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTIGSILPF 360
           SSLKLPVVLHQP+VG  MSDNPR+G NI+LPFQ+VP+S KVVGIL++NIY Q+  S  PF
Sbjct: 301 SSLKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPF 360

Query: 361 SLPPSFSLLPPQSDSVTMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHPDD 420
             PPSFSLLPPQ +S+  +LA   GKFSEV S GSLRLNSST+VKKSPIV+FNYYSHPDD
Sbjct: 361 FAPPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDD 420

Query: 421 VAKCVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEFCKKTVT 480
           +A+CV+G+RK+G+LL+TQTMEKIKT DLEG K FQFLG   P+N+S+D+SV E+C+KTVT
Sbjct: 421 LARCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVEEYCQKTVT 480

Query: 481 TYWHYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQ 540
           TYWHYHGGCLVGKVVD NY+VIGI+NLRVVDGSTF DSPGTNPMATLMMLGRYVG+K+LQ
Sbjct: 481 TYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFCDSPGTNPMATLMMLGRYVGLKVLQ 540

Query: 541 ERSS 544
           +RSS
Sbjct: 541 QRSS 542

[Close]
BLAST of Tan0012149 vs. ExPASy TrEMBL
Match: A0A6J1DIQ0 ((R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020835 PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 7.7e-252
Identity = 431/539 (79.96%), Postives = 483/539 (89.61%), Query Frame = 0 

Query: 3   HSLATFLLLILVSIFHFQLRVLPSHTLPNQDASYMKFVHDANDLTKKEEYDYIIIGGGTA 62
           +S+A FLLL +VSIFHFQL+VL SHT+PNQD SYMKFV DA DL +KEEYDYII+GGGTA
Sbjct: 4   YSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGGGTA 63

Query: 63  GCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVEN 122
           GCPLA TLSSKFSVLLLERGS PNK+PSVL E+ LLN F+ EDD KNPF RFISEDGVEN
Sbjct: 64  GCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDGVEN 123

Query: 123 IRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILSAWQSS 182
           +RGRVLGGSSMINAGFYSRGHRE+FE A V+WDMELVEK+YQWVE+TVVSRP LSA Q +
Sbjct: 124 LRGRVLGGSSMINAGFYSRGHREYFEKAGVEWDMELVEKAYQWVEETVVSRPSLSALQPT 183

Query: 183 FRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVVIHATV 242
           FRSALLE+GV+PDNGF LRHLVGT+IGGSIFD  GNRHGAVELLNKA P NLKV I ATV
Sbjct: 184 FRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNRHGAVELLNKANPTNLKVAIRATV 243

Query: 243 ERIIFSGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSL 302
           ERIIFSG+SA+GVSYSDS+  LHTAFIR KGEIILSAGAIGSPQLLLLSGIGPKS LSSL
Sbjct: 244 ERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQLSSL 303

Query: 303 KLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTIGSILPFSLP 362
           KLPVVLHQP+VG  MSDNPR+G +I+LPF  VPTSAKVVG LENNI+ QT+ S LPFS+P
Sbjct: 304 KLPVVLHQPYVGEFMSDNPRFGPSIVLPFPFVPTSAKVVGTLENNIHLQTVASPLPFSIP 363

Query: 363 PSFSLLPPQSDSVTMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHPDDVAK 422
           PSFSLLPPQS+SV ++L    GKFSEVLS GSLRLNSSTDVKKSPIV+FNYYSHPDD+A+
Sbjct: 364 PSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDDLAR 423

Query: 423 CVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEFCKKTVTTYW 482
           CV G+RK+G+L++T TMEKIKT+D EGKK FQF+GP  P+N+SDD++VGEFC K++TTYW
Sbjct: 424 CVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSLTTYW 483

Query: 483 HYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQER 542
           HYHGGCLVGKVVD +YRVIGI NLRVVDGSTF DSPGTNPMATLMMLGRYVG+K+LQ+R
Sbjct: 484 HYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFIDSPGTNPMATLMMLGRYVGLKVLQQR 542

[Close]
BLAST of Tan0012149 vs. ExPASy TrEMBL
Match: A0A6J1DGG8 ((R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020836 PE=3 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 4.2e-250
Identity = 428/539 (79.41%), Postives = 481/539 (89.24%), Query Frame = 0 

Query: 3   HSLATFLLLILVSIFHFQLRVLPSHTLPNQDASYMKFVHDANDLTKKEEYDYIIIGGGTA 62
           +S+A FLLL +VSIFHFQL+VL SHT+PNQD SYMKFV DA DL +KEEYDYII+GGGTA
Sbjct: 4   YSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGGGTA 63

Query: 63  GCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVEN 122
           GCPLA TLSSKFSVLLLERGS PNK+PSVL E+ LLN F+ EDD KNPF RFISEDGVEN
Sbjct: 64  GCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDGVEN 123

Query: 123 IRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILSAWQSS 182
           +RGRVLGGSSMINAGFYSRGH+EFFE A V+WDMELVEK+YQWVE+TVVSRP LSA Q +
Sbjct: 124 LRGRVLGGSSMINAGFYSRGHQEFFEKAGVEWDMELVEKAYQWVEETVVSRPSLSALQPT 183

Query: 183 FRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVVIHATV 242
           FRSALLE+GV+PDNGF LRHLVGT+IGGSIFD  GN HGAVELLNKA P N+KV I  TV
Sbjct: 184 FRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNPHGAVELLNKANPTNIKVAIRTTV 243

Query: 243 ERIIFSGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSL 302
           ERIIFSG+SA+GVSYSDS+  LHTAFIR KGEIILSAGAIGSPQLLLLSGIGPKS LSSL
Sbjct: 244 ERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQLSSL 303

Query: 303 KLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTIGSILPFSLP 362
           KLPVVLHQP+VG  MSDNPR+G +I+LPF  VPTSAKVVG LENNI+ QT+ S LPFS+P
Sbjct: 304 KLPVVLHQPYVGEFMSDNPRFGPSIVLPFPXVPTSAKVVGTLENNIHLQTVASPLPFSIP 363

Query: 363 PSFSLLPPQSDSVTMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHPDDVAK 422
           PSFSLLPPQS+SV ++L    GKFSEVLS GSLRLNSSTDVKKSPIV+FNYYSHPDD+A+
Sbjct: 364 PSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDDLAR 423

Query: 423 CVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEFCKKTVTTYW 482
           CV G+RK+G+L++T TMEKIKT+D EGKK FQF+GP  P+N+SDD++VGEFC K++TTYW
Sbjct: 424 CVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSLTTYW 483

Query: 483 HYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQER 542
           HYHGGCLVGKVVD +YRVIGI NLRVVDGSTF DSPGTNPMATLMMLGRYVG+K+LQ+R
Sbjct: 484 HYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFIDSPGTNPMATLMMLGRYVGLKVLQQR 542

[Close]
BLAST of Tan0012149 vs. ExPASy TrEMBL
Match: A0A6J1DH55 ((R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020834 PE=3 SV=1)

HSP 1 Score: 858.2 bits (2216), Expect = 1.8e-245
Identity = 417/539 (77.37%), Postives = 477/539 (88.50%), Query Frame = 0 

Query: 3   HSLATFLLLILVSIFHFQLRVLPSHTLPNQDASYMKFVHDANDLTKKEEYDYIIIGGGTA 62
           +S+ TFLLL ++SIFHFQL VL SHT+ NQD SYMKFVHDA DL +  EYDYII+GGG A
Sbjct: 4   NSMDTFLLLFIISIFHFQLGVLSSHTISNQDVSYMKFVHDAKDLPETGEYDYIIVGGGAA 63

Query: 63  GCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVEN 122
           GCPLA TLSSKFS+L+LERGS PNK+PSVL EQ     F+ EDDGKNPF RF+SEDGVEN
Sbjct: 64  GCPLATTLSSKFSILILERGSEPNKFPSVLTEQGRSKAFTAEDDGKNPFQRFVSEDGVEN 123

Query: 123 IRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILSAWQSS 182
           +RGRVLGG +MIN GFYSRGHREFF+TA V+WDMELVEK+YQWVE+TVVSRP LSAWQS+
Sbjct: 124 LRGRVLGGGTMINGGFYSRGHREFFDTAGVEWDMELVEKAYQWVEETVVSRPNLSAWQSA 183

Query: 183 FRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVVIHATV 242
           FRSALLE+GV+PDNGFDLRHLVGT+IGGS FD  GNRHGAVELLNKA P NLKVVI ATV
Sbjct: 184 FRSALLESGVLPDNGFDLRHLVGTRIGGSTFDGKGNRHGAVELLNKANPANLKVVIRATV 243

Query: 243 ERIIFSGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSL 302
           ERIIFSG+SA+GVSYSDS+  LHTAFIRKKGEI LSAGAIGSPQLLLLSG+GPKS LSSL
Sbjct: 244 ERIIFSGLSASGVSYSDSEGKLHTAFIRKKGEIFLSAGAIGSPQLLLLSGVGPKSDLSSL 303

Query: 303 KLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTIGSILPFSLP 362
           KLPVVLHQPHVG  MSDNPR+  NI+LPF IV T+ KVVG L+NNI+FQ+      FS+P
Sbjct: 304 KLPVVLHQPHVGQFMSDNPRFVTNIVLPFPIVSTTGKVVGTLKNNIHFQSFAGSSLFSVP 363

Query: 363 PSFSLLPPQSDSVTMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHPDDVAK 422
           PSFSLLPP+S+SV M+LAT+ GKFS+V S+GSLRLNSSTDVKKSPIV+FNYYSHPDD+A+
Sbjct: 364 PSFSLLPPRSNSVNMSLATVVGKFSKVDSMGSLRLNSSTDVKKSPIVRFNYYSHPDDLAR 423

Query: 423 CVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEFCKKTVTTYW 482
           CV G+RK+G+L++T TMEKIKT++ EGKK FQFLGP  P+N+SD +SVGEFC KT+TTYW
Sbjct: 424 CVVGVRKIGDLIKTPTMEKIKTRNFEGKKVFQFLGPPLPENLSDYSSVGEFCHKTLTTYW 483

Query: 483 HYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQER 542
           HYHGGCLVGKVVD +YRVIGI NLRVVDGSTF++SPGTNPMATLMMLGRYVG+K++Q+R
Sbjct: 484 HYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFTESPGTNPMATLMMLGRYVGLKVMQQR 542

[Close]
BLAST of Tan0012149 vs. ExPASy TrEMBL
Match: A0A6J1HRM5 ((R)-mandelonitrile lyase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465498 PE=3 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.6e-239
Identity = 414/539 (76.81%), Postives = 470/539 (87.20%), Query Frame = 0 

Query: 5   LATFLLLILVSIFHFQLRVLPSHTLPNQDASYMKFVHDANDLTKKEEYDYIIIGGGTAGC 64
           +AT L LIL+SIF   LRVL SHT+PNQD SYMK VHDA D+ + EEYDYIIIGGGTAGC
Sbjct: 1   MATILPLILISIFF--LRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGC 60

Query: 65  PLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVENIR 124
           PLAATLSSKFSVLLLERGS PNKYPSVL EQ LLN+F  EDDG+NPF RF+SE+GVE IR
Sbjct: 61  PLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIR 120

Query: 125 GRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILSAWQSSFR 184
           GRVLGG SM+NAGFYSRGHREFFE+A VDWDMELVEK Y+WVE+TVVSRP LSAWQ +FR
Sbjct: 121 GRVLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFR 180

Query: 185 SALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVVIHATVER 244
            ALLE GV PDNGFDLRHLVGTK GGSIFD++GNRHGAVELLN  EP NLKV + ATV++
Sbjct: 181 RALLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQK 240

Query: 245 IIFSGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKL 304
           IIFS +SA GVSYSDS+   HTAFIRKKGEIILSAGAIGSPQLLLLSG+GPKSHLSSLKL
Sbjct: 241 IIFSDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKL 300

Query: 305 PVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTIGSILPFSLPPS 364
           PVVLHQPHVG  MSDNPR+ A ++LPF +V +SA+VVGIL+ NI+ QT  S  PF + P+
Sbjct: 301 PVVLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPT 360

Query: 365 FSLLPPQSDSVTMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHPDDVAKCV 424
           F LLPPQS  +  +LA + GKFS+VLS GSL LNSSTDVK +PIV+FNYY++P+D+A+CV
Sbjct: 361 FGLLPPQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCV 420

Query: 425 RGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEFCKKTVTTYWHY 484
           RGLRK+G+ L+T+TME+IKTQDLEG K FQFLGP  P+N+SD++SV E+CK+TVTTYWHY
Sbjct: 421 RGLRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHY 480

Query: 485 HGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQERSS 544
           HGGCLVGKVVD NYRVIGIKNLRVVDGSTFS SPGTNPMATLMMLGRYVG+K+LQERSS
Sbjct: 481 HGGCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 537

[Close]
BLAST of Tan0012149 vs. ExPASy TrEMBL
Match: A0A6J1FC90 ((R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111444087 PE=3 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 2.2e-238
Identity = 414/543 (76.24%), Postives = 472/543 (86.92%), Query Frame = 0 

Query: 1   MDHSLATFLLLILVSIFHFQLRVLPSHTLPNQDASYMKFVHDANDLTKKEEYDYIIIGGG 60
           ++HS+AT L LIL+SI  F LRVL S+T+PNQD SYMK VHDA +L + E+YDYIIIGGG
Sbjct: 4   IEHSMATILPLILISI--FLLRVLSSNTIPNQDVSYMKLVHDATELPENEDYDYIIIGGG 63

Query: 61  TAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGV 120
           TAGCPLAATLSSKFSVLLLERGS PNKYPSVL EQ LLN+F  EDDG+NPF RF+SEDGV
Sbjct: 64  TAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSEDGV 123

Query: 121 ENIRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILSAWQ 180
           E IRGRVLGG SM+NAGFYSRGH EFFE+A VDWDMELVEK Y+WVE+TVVSRP LSAWQ
Sbjct: 124 EIIRGRVLGGGSMVNAGFYSRGHGEFFESAGVDWDMELVEKGYEWVEKTVVSRPRLSAWQ 183

Query: 181 SSFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVVIHA 240
           S+FR ALLE GV PDNGFDLRHLVGTK GGSIFD++GNRHGAVELLN  EP NLKV + A
Sbjct: 184 SAFRRALLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRA 243

Query: 241 TVERIIFSGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLS 300
           TV++IIFS +SA GVSYSDSK   HTAFIRKKGEIILSAGAIGSPQLLLLSG+GPKSHLS
Sbjct: 244 TVQKIIFSDLSAIGVSYSDSKGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLS 303

Query: 301 SLKLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTIGSILPFS 360
           SLKLPVVLHQPHVG  MSDNPR+ A I+LPF +V +SA+VVGIL+ NI+ QT  S  PF 
Sbjct: 304 SLKLPVVLHQPHVGEFMSDNPRFSAAIVLPFPLVASSAQVVGILDGNIHLQTFASPAPFF 363

Query: 361 LPPSFSLLPPQSDSVTMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHPDDV 420
           + P+F LLPPQS  +  +LA + GKF++VLS GSL LNSSTDVK +PIV+FNYYS+P+D+
Sbjct: 364 ILPTFGLLPPQSTLINPSLALLIGKFTDVLSKGSLHLNSSTDVKNNPIVRFNYYSNPNDL 423

Query: 421 AKCVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEFCKKTVTT 480
           A+CVRGLRK+G+LL+T+ MEKIKT+DLEG K F FLGP  P+N+SD +SV E+CK+TVTT
Sbjct: 424 AQCVRGLRKVGDLLKTRMMEKIKTRDLEGNKGFLFLGPPLPENLSDISSVEEYCKETVTT 483

Query: 481 YWHYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQE 540
           YWHYHGGCLVGKVVD NYRVIG++NLRVVDGSTFS SPGTNPMATLMMLGRYVG+K+LQE
Sbjct: 484 YWHYHGGCLVGKVVDGNYRVIGVENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQE 543

Query: 541 RSS 544
           RSS
Sbjct: 544 RSS 544

[Close]
BLAST of Tan0012149 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 525.0 bits (1351), Expect = 7.1e-149
Identity = 265/553 (47.92%), Postives = 372/553 (67.27%), Query Frame = 0 

Query: 1   MDHSL--ATFLLLILVSIFHFQLRVLPSHTLP--NQDASYMKFVHDANDLTKKEEYDYII 60
           +D SL     ++L+L+ + H       S+  P  N+   +M+F+ +A D   ++ YDYII
Sbjct: 5   IDSSLLYTALVVLLLLGVVH------RSNARPRVNRPPGFMRFISNATDFASEDYYDYII 64

Query: 61  IGGGTAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFIS 120
           +GGGTAGCPLAATLS  F VLLLERG VP   P+V+     L   +  ++  +P   FIS
Sbjct: 65  VGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFIS 124

Query: 121 EDGVENIRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPIL 180
           E+GV N RGRVLGGSS INAGFYSR  ++FFE + + WD+  V +SY+WVE+ +V RP L
Sbjct: 125 EEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQL 184

Query: 181 SAWQSSFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKV 240
             WQ++ R ALLE GV P NGF L H VGTKIGGS FD  G RH + +LL  A   N++V
Sbjct: 185 RTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRV 244

Query: 241 VIHATVERIIF--------SGVSATGVSYSDSKSNLHTAFIRKKGEIILSAGAIGSPQLL 300
            ++ATVER++         S VSA GV Y D     H A IR +GE+ILSAGA+GSPQLL
Sbjct: 245 AVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLL 304

Query: 301 LLSGIGPKSHLSSLKLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNI 360
            LSGIGP+S+LS+  +PV L QPHVG+ + DNPR G +I+ P  +  +  +VVG+ E+  
Sbjct: 305 FLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGA 364

Query: 361 YFQTIGSILPFSLPPSFSLLPPQSDSVTMTLATIFGKFSEVLSVGSLRLNSSTDVKKSPI 420
           + +   +++PF+ P     +   +  + + + TI  K    +S+G LRL +STDV+ +P+
Sbjct: 365 FLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPV 424

Query: 421 VQFNYYSHPDDVAKCVRGLRKLGELLQTQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDN 480
           V+FNY+S P D+ +CV G RK+GE+L+++ M+    ++  G + F+F+G   P + S+D 
Sbjct: 425 VRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDL 484

Query: 481 SVGEFCKKTVTTYWHYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMM 540
            + +FC++TV+T WHYHGG +VGKVVDS+ +VIG+ +LR+VDGSTF+ SPGTNP ATLMM
Sbjct: 485 VMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMM 544

Query: 541 LGRYVGVKILQER 542
           LGRY+G+K+L+ER
Sbjct: 545 LGRYMGLKMLRER 550

[Close]
BLAST of Tan0012149 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 413.3 bits (1061), Expect = 3.0e-115
Identity = 242/572 (42.31%), Postives = 325/572 (56.82%), Query Frame = 0 

Query: 10  LLILVSIFHFQLRVLPSHTLPNQDASYMKFVHDANDLTKKEEYDYIIIGGGTAGCPLAAT 69
           L + +S+F      L S    +  A    F+ DA        YDYIIIGGGTAGCPLAAT
Sbjct: 10  LFVALSLF------LHSPICSSDKAPNYSFMRDATGSPTTSYYDYIIIGGGTAGCPLAAT 69

Query: 70  LSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFS---TEDDGKNPFNRFISEDGVENIRGR 129
           LS   SVLLLERG  P   P++ +    L+ F    ++    +P  RF+SEDGV N R R
Sbjct: 70  LSQNASVLLLERGDSPYNNPNITR----LSAFGAALSDLSESSPSQRFVSEDGVINARAR 129

Query: 130 VLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQTVVSRPILSAWQSSFRSA 189
           VLGG S +NAGFY+R   ++    ++ WD  L  +SYQWVE  V  +P +  WQ++ R  
Sbjct: 130 VLGGGSALNAGFYTRAGTKY--VRNMGWDGALANESYQWVEAKVAFQPPMGRWQTAVRDG 189

Query: 190 LLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKAEPENLKVVIHATVERII 249
           LLEAG+VP+NGF   H+ GTK GG+IFD NGNRH A +LL  A+P+ + V++HATV RI+
Sbjct: 190 LLEAGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRIL 249

Query: 250 F-----SGVSATGVSYSDSKSNLHTAFIRK--KGEIILSAGAIGSPQLLLLSGIGPKSHL 309
           F     +   A GV Y D     H A++++    EIILSAG +GSPQLL+LSG+GP + L
Sbjct: 250 FRTRGTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQL 309

Query: 310 SSLKLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGILENNIYFQTI------ 369
            +  + VV+ QPHVG  M DNP     +  P  +  +  +VVGI     Y +        
Sbjct: 310 QAQNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFG 369

Query: 370 ----GSILPFSLPPSFSLLPP-----QSDSVTMTLAT-------IFGKFSEVLSVGSLRL 429
               GS    S    +++  P     +S+S+T   +        +  K    LS G L L
Sbjct: 370 GGGGGSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLEL 429

Query: 430 NSSTDVKKSPIVQFNYYSHPDDVAKCVRGLRKLGELLQTQTMEKIKTQD--------LEG 489
             + + K +PIV FNY+ HPDD+ +CVRG++ +  ++Q++   + K  D        L  
Sbjct: 430 -KTRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADVSFEYLLNLTA 489

Query: 490 KKTFQFLGPMFPKNMSDDNSVGEFCKKTVTTYWHYHGGCLVGKVVDSNYRVIGIKNLRVV 542
                   P      S   S  EFC+ TVTT WHYHGGC+VG+VVD +Y+VIGI  LRV+
Sbjct: 490 STPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVI 549

[Close]
BLAST of Tan0012149 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 408.3 bits (1048), Expect = 9.7e-114
Identity = 239/531 (45.01%), Postives = 314/531 (59.13%), Query Frame = 0 

Query: 52  YDYIIIGGGTAGCPLAATLSSKFSVLLLERGSVP--NKYPSVLKEQDLLNIFSTEDDGKN 111
           YDYI+IGGGTAGCPLAATLS  FSVL+LERG VP  N   S L+     +I   +    +
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADISASS 122

Query: 112 PFNRFISEDGVENIRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQT 171
               F+S DGV N R RVLGG S INAGFYSR    F + A   WD +LV++SY WVE+ 
Sbjct: 123 ASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRA--GWDPKLVKESYPWVERE 182

Query: 172 VVSRPILSAWQSSFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKA 231
           +V +P L+ WQ + R +LLE GV P NGF   H+ GTKIGG+IFD  G RH A ELL  A
Sbjct: 183 IVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYA 242

Query: 232 EPENLKVVIHATVERIIF--SGV--SATGVSYSDSKSNLHTAFI--RKKGEIILSAGAIG 291
            P+ L+V+I+ATV++I+F  SG     TGV + D K N H A +  RK  E+ILS+GAIG
Sbjct: 243 NPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIG 302

Query: 292 SPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGI 351
           SPQ+L+LSGIGPK  L  LK+PVVL   HVG  M+DNP     +     I  +  + VGI
Sbjct: 303 SPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGI 362

Query: 352 LENNIYFQTIGSI--LPFSLPPS----------FSLLP-----PQSDSVTMTL------- 411
            +  +Y +        P S+             FS +P     P++    +T        
Sbjct: 363 TKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHE 422

Query: 412 ----ATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHPDDVAKCVRGLRKLGELLQ 471
               + I  K +  +S G L L  +T+V  +P V FNY+ HP D+ +CV  +R + +++ 
Sbjct: 423 AFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVT 482

Query: 472 TQTMEKIKTQDLEGKKTFQFLG-----PMFPKNMSDDNSVGEFCKKTVTTYWHYHGGCLV 531
           +         D +       L       + PK ++D  S+ +FCK TV T WHYHGGCLV
Sbjct: 483 SNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLV 542

Query: 532 GKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQER 542
           GKVV  N +V+G+  LRV+DGSTF +SPGTNP AT+MM+GRY+GVKIL+ER
Sbjct: 543 GKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 587

[Close]
BLAST of Tan0012149 vs. TAIR 10
Match: AT1G72970.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 394.8 bits (1013), Expect = 1.1e-109
Identity = 236/526 (44.87%), Postives = 307/526 (58.37%), Query Frame = 0 

Query: 52  YDYIIIGGGTAGCPLAATLSSKFSVLLLERGSVP--NKYPSVLKEQDLLNIFSTEDDGKN 111
           YDYI+IGGGTAGCPLAATLS  FSVL+LERG VP  N   S L+     +I   +    +
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADISASS 122

Query: 112 PFNRFISEDGVENIRGRVLGGSSMINAGFYSRGHREFFETADVDWDMELVEKSYQWVEQT 171
               F+S DGV N R RVLGG S INAGFYSR    F + A   WD +LV++SY WVE+ 
Sbjct: 123 ASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRA--GWDPKLVKESYPWVERE 182

Query: 172 VVSRPILSAWQSSFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDNGNRHGAVELLNKA 231
           +V +P L+ WQ + R +LLE GV P NGF   H+ GTKIGG+IFD  G RH A ELL  A
Sbjct: 183 IVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYA 242

Query: 232 EPENLKVVIHATVERIIF--SGV--SATGVSYSDSKSNLHTAFI--RKKGEIILSAGAIG 291
            P+ L+V+I+ATV++I+F  SG     TGV + D K N H A +  RK  E+ILS+GAIG
Sbjct: 243 NPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIG 302

Query: 292 SPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGNSMSDNPRYGANIMLPFQIVPTSAKVVGI 351
           SPQ+L+LSGIGPK  L  LK+PVVL   HVG  M+DNP     +     I  +  + VGI
Sbjct: 303 SPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGI 362

Query: 352 LENNIYFQTIGSI--LPFSLPPS----------FSLLP-----PQSDSVTMTL------- 411
            +  +Y +        P S+             FS +P     P++    +T        
Sbjct: 363 TKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHE 422

Query: 412 ----ATIFGKFSEVLSVGSLRLNSSTDVKKSPIVQFNYYSHPDDVAKCVRGLRKLGELLQ 471
               + I  K +  +S G L L  +T+V  +P V FNY+ HP D                
Sbjct: 423 AFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPCD---------------- 482

Query: 472 TQTMEKIKTQDLEGKKTFQFLGPMFPKNMSDDNSVGEFCKKTVTTYWHYHGGCLVGKVVD 531
            Q + K+ +  ++     +      PK ++D  S+ +FCK TV T WHYHGGCLVGKVV 
Sbjct: 483 KQNVHKMLSLSVKANINLR------PKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKVVS 542

Query: 532 SNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQER 542
            N +V+G+  LRV+DGSTF +SPGTNP AT+MM+GRY+GVKIL+ER
Sbjct: 543 PNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 560

[Close]
BLAST of Tan0012149 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 386.3 bits (991), Expect = 3.9e-107
Identity = 232/553 (41.95%), Postives = 317/553 (57.32%), Query Frame = 0 

Query: 34  ASYMKFVHDANDLTKKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGSVPNKYPSVLK 93
           A Y  F+ DA        +DYIIIGGGT+GC LAATLS   SVL+LERG  P   P+   
Sbjct: 28  AGYYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATD 87

Query: 94  EQDLLNIFSTEDDGKNPFNRFISEDGVENIRGRVLGGSSMINAGFYSRGHREFFETADVD 153
            ++     S     K+    FISEDGV N R RVLGG S++NAGFY+R   E+ +  + +
Sbjct: 88  IENFATTLSNTSP-KSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVK--ETE 147

Query: 154 WDMELVEKSYQWVEQTVVSRPILSAWQSSFRSALLEAGVVPDNGFDLRHLVGTKIGGSIF 213
           W  + VE +Y+WVE+ V  +P +  WQ++F+  LLEAG  P NGF   H+ GTKIGG+IF
Sbjct: 148 WKTDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIF 207

Query: 214 DDNGNRHGAVELLNKAEPENLKVVIHATVERIIFS-----GVSATGVSYSDSKSNLHTAF 273
           D  G+RH A +LL  A P N+ V +HA+V +I+F+        A GV + D+   LH A 
Sbjct: 208 DRAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAE 267

Query: 274 IRKK--GEIILSAGAIGSPQLLLLSGIGPKSHLSSLKL-PVVLHQPHVGNSMSDNPRYGA 333
           + K    E+ILSAGAIGSPQLL+LSGIGP +HL++  + P+VL  P VG  M DNP    
Sbjct: 268 LEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAI 327

Query: 334 NIMLPFQIVPTSAKVVGILENNIYFQ-TIGSILPFSLPPSF---------SLLPPQSDSV 393
            I  P  +  +  +VVGI +   Y +   G I  +S    F          +   ++ S 
Sbjct: 328 FIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTST 387

Query: 394 TM-TLAT----------------------IFGKFSEVLSVGSLRLNSSTDVKKSPIVQFN 453
           T  TL+T                      I  K +  +S G L L  +T+   +P V+FN
Sbjct: 388 TSPTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELR-NTNPDDNPSVRFN 447

Query: 454 YYSHPDDVAKCVRGLRKLGELLQTQTMEKIKTQD--LEGKKTFQFLGP--MFPKNMSDDN 513
           YY  P+D+  CV G+  + +++ ++   K K  D  + G        P  + P++++   
Sbjct: 448 YYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLMLSVPTNLRPRHITSMF 507

Query: 514 SVGEFCKKTVTTYWHYHGGCLVGKVVDSNYRVIGIKNLRVVDGSTFSDSPGTNPMATLMM 542
           ++ +FC  TV T WHYHGGC VG+VVD NYRV+GI +LRV+DGSTF  SPGTNP AT+MM
Sbjct: 508 NLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMM 567

The following BLAST results are available for this feature:
BLAST of Tan0012149 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P527062.8e-15852.36(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q945K22.6e-15651.38(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
P527072.1e-15350.18(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
O500484.1e-14949.18(R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1[more]
Q9SSM21.0e-14747.92(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ... [more]
BLAST of Tan0012149 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022153314.11.6e-25179.96(R)-mandelonitrile lyase 1-like [Momordica charantia][more]
XP_022153315.18.7e-25079.41(R)-mandelonitrile lyase 1-like [Momordica charantia][more]
XP_038889314.14.5e-24678.27(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
XP_022153313.13.8e-24577.37(R)-mandelonitrile lyase 1-like [Momordica charantia][more]
XP_038889315.15.0e-24577.21(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
BLAST of Tan0012149 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DIQ07.7e-25279.96(R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020835 P... [more]
A0A6J1DGG84.2e-25079.41(R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020836 P... [more]
A0A6J1DH551.8e-24577.37(R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020834 P... [more]
A0A6J1HRM52.6e-23976.81(R)-mandelonitrile lyase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465498 PE=3... [more]
A0A6J1FC902.2e-23876.24(R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111444087 PE... [more]
BLAST of Tan0012149 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G73050.17.1e-14947.92Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.13.0e-11542.31Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.19.7e-11445.01Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.21.1e-10944.87Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.13.9e-10741.95Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 349..543
e-value: 1.1E-33
score: 114.8
coord: 35..351
e-value: 1.6E-59
score: 200.1
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 391..531
e-value: 1.1E-28
score: 100.5
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 52..530
e-value: 6.1E-171
score: 571.6
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 36..540
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 52..321
e-value: 5.1E-33
score: 114.7
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 125..148
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 280..294
NoneNo IPR availableGENE3D3.30.410.40coord: 172..480
e-value: 6.1E-171
score: 571.6
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 7..542
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 7..542
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 372..487

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0012149.1Tan0012149.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors