Tan0012103 (gene) Snake gourd v1

BLAST of Tan0012103 vs. ExPASy Swiss-Prot
Match: Q7TXM1 (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=fadD26 PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 6.6e-42
Identity = 160/579 (27.63%), Postives = 259/579 (44.73%), Query Frame = 0

Query: 55  LTYKQLHDSVQFISDSLLRQLRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPC 114
           LT+ Q++     I++ L       D V +L   GL+ V    G  +AG ++VP+S P   
Sbjct: 41  LTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQ-- 100

Query: 115 FGNENYDHLARALSQTKPRAAIAHQAYIARVFRYLTSSPIDEKLASLLQSVQWISMEILK 174
           +G  + D ++  L  +KP A +   + +  V +Y  S   D + A ++  V  + +    
Sbjct: 101 YGIHD-DRVSAVLQDSKPVAILTTSSVVGDVTKYAASH--DGQPAPVVVEVDLLDL---- 160

Query: 175 PCNDANQKPSLISNNHQPNFHPSSYHGCKPDEPYLIQYTSGATGVSKPVVVTAGAAVHNV 234
              D+ ++    S  H      ++Y          +QYTSG+T     V+V+    + NV
Sbjct: 161 ---DSPRQMPAFSRQHT----GAAY----------LQYTSGSTRTPAGVIVSHTNVIANV 220

Query: 235 RAARKSYDLN----PNAVVVSWLPQYHDCGLMF-LLLTVVTGATCVLTSPISFVTRPITW 294
             +   Y  +    P   VVSWLP YHD GL+  +   +V     VL SP+SF+ RP  W
Sbjct: 221 TQSMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAVLMSPMSFLRRPARW 280

Query: 295 LHLITAFRATCTPVPSFTLPLVLKRVKEDPVVNITGLDLGSLKNLILINEPIYRSAVEEF 354
           + L+       +  P+F   L ++R  +    ++ GLDL  +  ++  +E I+ + V  F
Sbjct: 281 MQLLATSGRCFSAAPNFAFELAVRRTSDQ---DMAGLDLRDVVGIVSGSERIHVATVRRF 340

Query: 355 VEVFKAVGLDPGCVSPSYGLAENCTFVSTAWSGGGRRWWFPAMPSYRKLLPSARL---GE 414
           +E F    L P  + PSYGLAE   +V+   +G   +        Y +L          +
Sbjct: 341 IERFAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPK---TVRFDYEQLTAGQARPCGTD 400

Query: 415 GSVEIEVV-----------VVNAETREVVDDGVEGEIWV----------SSPSNASETFH 474
           GSV  E++           +VN ET      GV GEIWV            P   ++ F 
Sbjct: 401 GSVGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFD 460

Query: 475 SKLSN--KSSPN--FVRTGDRGVIKGADRYLFVTGRCSDVIKLQNNQEIHPHYIESVAYN 534
           +KL +   ++P   ++RTGD GVI  +D  LF+ GR  D++ + + +  +P  IE+    
Sbjct: 461 AKLVDPAPAAPEGPWLRTGDLGVI--SDGELFIMGRIKDLL-IVDGRNHYPDDIEATI-- 520

Query: 535 NFPAHLRGGCLAAF----KISETIAVVAEMQRFDKNDGESM---RRICEGIRKAVLIEEG 594
                + GG  AA      I+E +  + E +R      E M   R +   +  A+     
Sbjct: 521 ---QEITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHS 579

BLAST of Tan0012103 vs. ExPASy Swiss-Prot
Match: P95141 (4-hydroxyphenylalkanoate adenylyltransferase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=fadD29 PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 8.6e-42
Identity = 159/594 (26.77%), Postives = 263/594 (44.28%), Query Frame = 0

Query: 29  FRSKPAFIWSEDGTADAGLNEEGSFLLTYKQLHDSVQFISDSLLRQLRRRDTVVILCSPG 88
           + ++ A+ + +  T  AG  E     +T+ Q+H     +++ L       D V IL   G
Sbjct: 54  YPNRAAYKFIDYDTDPAGFTE----TVTWWQVHRRAMIVAEELWIYASSGDRVAILAPQG 113

Query: 89  LDLVQLIYGCQRAGLVSVPISPPDPCFGNENYDHLARALSQTKPRAAIAHQAYIARVFRY 148
           L+ +    G  +AGL++VP+  P P FG  + + ++ AL  + P   +   + I  V  Y
Sbjct: 114 LEYIIAFMGVLQAGLIAVPL--PVPQFGIHD-ERISSALRDSAPSIILTTSSVIDEVTTY 173

Query: 149 LT-SSPIDEKLASLLQSVQWISMEILKPCNDANQKPSLISNNHQPNFHPSSYHGCKPDEP 208
              +     + A ++ +V  + +   +  +                  P+ +   +P   
Sbjct: 174 APHACAAQGQSAPIVVAVDALDLSSSRALD------------------PTRFE--RPSTA 233

Query: 209 YLIQYTSGATGVSKPVVVTAGAAVHNVRAARKSYDLNPNAV---VVSWLPQYHDCGLMF- 268
           YL QYTSG+T     VV++    + N       Y  +   V    VSWLP YHD GLM  
Sbjct: 234 YL-QYTSGSTRAPAGVVLSHKNVITNCVQLMSDYIGDSEKVPSTPVSWLPFYHDMGLMLG 293

Query: 269 LLLTVVTGATCVLTSPISFVTRPITWLHLITAFRATCTPVPSFTLPLVLKRVKEDPVVNI 328
           ++L ++   T VL SP++F+ RP  W+ L+   RA  +  P+F   L ++R  +D   ++
Sbjct: 294 IILPMINQDTAVLMSPMAFLQRPARWMQLLAKHRAQISSAPNFGFELAVRRTSDD---DM 353

Query: 329 TGLDLGSLKNLILINEPIYRSAVEEFVEVFKAVGLDPGCVSPSYGLAENCTFVSTAWSGG 388
            GLDLG ++ ++   E +  + +  F E F    L    + PSYGLAE   +V+TA  G 
Sbjct: 354 AGLDLGHVRTIVTGAERVNVATLRRFTERFAPFNLSETAIRPSYGLAEATVYVATA--GP 413

Query: 389 GRRWWFPAMPSYRKLLPSARLGEGSVE------------IEVVVVNAETREVVDDGVEGE 448
           GR          +  +  A+  E   E              V +V+ ETR     G  GE
Sbjct: 414 GRAPKSVCFDYQQLSVGQAKRAENGSEGANLVSYGAPRASTVRIVDPETRMENPAGTVGE 473

Query: 449 IWVS----------SPSNASETFHSKL----SNKSSPNFVRTGDRGVIKGADRYLFVTGR 508
           IWV           +P     TF ++L       S   ++RTGD GVI   +  LF+TGR
Sbjct: 474 IWVQGDNVGLGYWRNPQQTEATFRARLVTPSPGTSEGPWLRTGDLGVIFEGE--LFITGR 533

Query: 509 CSDVIKLQNNQEIHPHYIESVAYNNFPAHLRGGCLAAFKISE-------TIAVVAEMQRF 568
             +++ +      +P  IE+         + GG + A  + +       TI  + +  R 
Sbjct: 534 IKELLVVDGANH-YPEDIEATI-----QEITGGRVVAIAVPDDRTEKLVTIIELMKRGRT 593

Query: 569 DKNDGESMRRICEGIRKAVLIEEGIELGLVVLVKRGNVPKTTSGKVMRSAAKER 585
           D+ +   +R +   +  A+     + +  VV+V  G++P TTSGKV RSA+ ER
Sbjct: 594 DEEEKNRLRTVKREVASAISRSHRLRVADVVMVAPGSIPVTTSGKVRRSASVER 606

BLAST of Tan0012103 vs. ExPASy Swiss-Prot
Match: Q7TXK5 (4-hydroxyphenylalkanoate adenylyltransferase OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=fadD29 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 1.1e-41
Identity = 159/595 (26.72%), Postives = 265/595 (44.54%), Query Frame = 0

Query: 29  FRSKPAFIWSEDGTADAGLNEEGSFLLTYKQLHDSVQFISDSLLRQLRRRDTVVILCSPG 88
           + ++ A+ + +  T  AG  E     +T+ Q+H     +++ L       D V IL   G
Sbjct: 54  YPNRAAYKFIDYDTDPAGFTE----TVTWWQVHRRAMIVAEELWIYASSGDRVAILAPQG 113

Query: 89  LDLVQLIYGCQRAGLVSVPISPPDPCFGNENYDHLARALSQTKPRAAIAHQAYIARVFRY 148
           L+ +    G  +AGL++VP+  P P FG  + + ++ AL  + P   +   + I  V  Y
Sbjct: 114 LEYIIAFMGVLQAGLIAVPL--PVPQFGIHD-ERISSALRDSAPSIILTTSSVIDEVTTY 173

Query: 149 LT-SSPIDEKLASLLQSVQWISMEILKPCNDANQKPSLISNNHQPNFHPSSYHGCKPDEP 208
              +     + A ++ +V  + +   +  +                  P+ +   +P   
Sbjct: 174 APHACAAQGQSAPIVVAVDALDLSSSRALD------------------PTRFE--RPSTA 233

Query: 209 YLIQYTSGATGVSKPVVVTAGAAVHNVRAARKSYDLNPNAV---VVSWLPQYHDCGLMF- 268
           YL QYTSG+T     VV++    + N       Y  +   V    VSWLP YHD GLM  
Sbjct: 234 YL-QYTSGSTRAPAGVVLSHKNVITNCVQLMSDYIGDSEKVPSTPVSWLPFYHDMGLMLG 293

Query: 269 LLLTVVTGATCVLTSPISFVTRPITWLHLITAFRATCTPVPSFTLPLVLKRVKEDPVVNI 328
           ++L ++   T VL SP++F+ RP  W+ L+   RA  +  P+F   L ++R  +D   ++
Sbjct: 294 IILPMINQDTAVLMSPMAFLQRPARWMQLLAKHRAQISSAPNFGFELAVRRTSDD---DM 353

Query: 329 TGLDLGSLKNLILINEPIYRSAVEEFVEVFKAVGLDPGCVSPSYGLAENCTFVSTAWSGG 388
            GLDLG ++ ++   E +  + +  F E F    L    + PSYGLAE   +V+TA  G 
Sbjct: 354 AGLDLGHVRTIVTGAERVNVATLRRFTERFAPFNLSETAIRPSYGLAEATVYVATAGPGR 413

Query: 389 GRRWWFPAMPSYRKLL--PSARLGEGSVEIEVV-----------VVNAETREVVDDGVEG 448
             +        Y++L    + R   GS    +V           +V+ ETR     G  G
Sbjct: 414 APK---SVCFDYQQLSVGQAKRTENGSEGANLVSYGAPRASTVRIVDPETRMENPAGTVG 473

Query: 449 EIWVS----------SPSNASETFHSKL----SNKSSPNFVRTGDRGVIKGADRYLFVTG 508
           EIWV           +P     TF ++L       S   ++RTGD GVI   +  LF+TG
Sbjct: 474 EIWVQGDNVGLGYWRNPQQTEATFRARLVTPSPGTSEGPWLRTGDLGVIFEGE--LFITG 533

Query: 509 RCSDVIKLQNNQEIHPHYIESVAYNNFPAHLRGGCLAAFKISE-------TIAVVAEMQR 568
           R  +++ +      +P  IE+         + GG + A  + +       TI  + +  R
Sbjct: 534 RIKELLVVDGANH-YPEDIEATI-----QEITGGRVVAIAVPDDRTEKLVTIIELMKRGR 593

Query: 569 FDKNDGESMRRICEGIRKAVLIEEGIELGLVVLVKRGNVPKTTSGKVMRSAAKER 585
            D+ +   +R +   +  A+     + +  VV+V  G++P TTSGKV RSA+ ER
Sbjct: 594 TDEEEKNRLRTVKREVASAISRSHRLRVADVVMVAPGSIPVTTSGKVRRSASVER 606

BLAST of Tan0012103 vs. ExPASy Swiss-Prot
Match: P9WQ42 (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=fadD26 PE=3 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 1.5e-41
Identity = 159/579 (27.46%), Postives = 259/579 (44.73%), Query Frame = 0

Query: 55  LTYKQLHDSVQFISDSLLRQLRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPC 114
           LT+ Q++     I++ L       D V +L   GL+ V    G  +AG ++VP+S P   
Sbjct: 41  LTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQ-- 100

Query: 115 FGNENYDHLARALSQTKPRAAIAHQAYIARVFRYLTSSPIDEKLASLLQSVQWISMEILK 174
           +G  + D ++  L  +KP A +   + +  V +Y  S   D + A ++  V  + +    
Sbjct: 101 YGIHD-DRVSAVLQDSKPVAILTTSSVVGDVTKYAASH--DGQPAPVVVEVDLLDL---- 160

Query: 175 PCNDANQKPSLISNNHQPNFHPSSYHGCKPDEPYLIQYTSGATGVSKPVVVTAGAAVHNV 234
              D+ ++    S  H      ++Y          +QYTSG+T     V+V+    + NV
Sbjct: 161 ---DSPRQMPAFSRQHT----GAAY----------LQYTSGSTRTPAGVIVSHTNVIANV 220

Query: 235 RAARKSYDLN----PNAVVVSWLPQYHDCGLMF-LLLTVVTGATCVLTSPISFVTRPITW 294
             +   Y  +    P   VVSWLP YHD GL+  +   +V     +L SP+SF+ RP  W
Sbjct: 221 TQSMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAMLMSPMSFLRRPARW 280

Query: 295 LHLITAFRATCTPVPSFTLPLVLKRVKEDPVVNITGLDLGSLKNLILINEPIYRSAVEEF 354
           + L+       +  P+F   L ++R  +    ++ GLDL  +  ++  +E I+ + V  F
Sbjct: 281 MQLLATSGRCFSAAPNFAFELAVRRTSDQ---DMAGLDLRDVVGIVSGSERIHVATVRRF 340

Query: 355 VEVFKAVGLDPGCVSPSYGLAENCTFVSTAWSGGGRRWWFPAMPSYRKLLPSARL---GE 414
           +E F    L P  + PSYGLAE   +V+   +G   +        Y +L          +
Sbjct: 341 IERFAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPK---TVRFDYEQLTAGQARPCGTD 400

Query: 415 GSVEIEVV-----------VVNAETREVVDDGVEGEIWV----------SSPSNASETFH 474
           GSV  E++           +VN ET      GV GEIWV            P   ++ F 
Sbjct: 401 GSVGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFD 460

Query: 475 SKLSN--KSSPN--FVRTGDRGVIKGADRYLFVTGRCSDVIKLQNNQEIHPHYIESVAYN 534
           +KL +   ++P   ++RTGD GVI  +D  LF+ GR  D++ + + +  +P  IE+    
Sbjct: 461 AKLVDPAPAAPEGPWLRTGDLGVI--SDGELFIMGRIKDLL-IVDGRNHYPDDIEATI-- 520

Query: 535 NFPAHLRGGCLAAF----KISETIAVVAEMQRFDKNDGESM---RRICEGIRKAVLIEEG 594
                + GG  AA      I+E +  + E +R      E M   R +   +  A+     
Sbjct: 521 ---QEITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHS 579

BLAST of Tan0012103 vs. ExPASy Swiss-Prot
Match: P9WQ43 (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=fadD26 PE=1 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 1.5e-41
Identity = 159/579 (27.46%), Postives = 259/579 (44.73%), Query Frame = 0

Query: 55  LTYKQLHDSVQFISDSLLRQLRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPC 114
           LT+ Q++     I++ L       D V +L   GL+ V    G  +AG ++VP+S P   
Sbjct: 41  LTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQ-- 100

Query: 115 FGNENYDHLARALSQTKPRAAIAHQAYIARVFRYLTSSPIDEKLASLLQSVQWISMEILK 174
           +G  + D ++  L  +KP A +   + +  V +Y  S   D + A ++  V  + +    
Sbjct: 101 YGIHD-DRVSAVLQDSKPVAILTTSSVVGDVTKYAASH--DGQPAPVVVEVDLLDL---- 160

Query: 175 PCNDANQKPSLISNNHQPNFHPSSYHGCKPDEPYLIQYTSGATGVSKPVVVTAGAAVHNV 234
              D+ ++    S  H      ++Y          +QYTSG+T     V+V+    + NV
Sbjct: 161 ---DSPRQMPAFSRQHT----GAAY----------LQYTSGSTRTPAGVIVSHTNVIANV 220

Query: 235 RAARKSYDLN----PNAVVVSWLPQYHDCGLMF-LLLTVVTGATCVLTSPISFVTRPITW 294
             +   Y  +    P   VVSWLP YHD GL+  +   +V     +L SP+SF+ RP  W
Sbjct: 221 TQSMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAMLMSPMSFLRRPARW 280

Query: 295 LHLITAFRATCTPVPSFTLPLVLKRVKEDPVVNITGLDLGSLKNLILINEPIYRSAVEEF 354
           + L+       +  P+F   L ++R  +    ++ GLDL  +  ++  +E I+ + V  F
Sbjct: 281 MQLLATSGRCFSAAPNFAFELAVRRTSDQ---DMAGLDLRDVVGIVSGSERIHVATVRRF 340

Query: 355 VEVFKAVGLDPGCVSPSYGLAENCTFVSTAWSGGGRRWWFPAMPSYRKLLPSARL---GE 414
           +E F    L P  + PSYGLAE   +V+   +G   +        Y +L          +
Sbjct: 341 IERFAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPK---TVRFDYEQLTAGQARPCGTD 400

Query: 415 GSVEIEVV-----------VVNAETREVVDDGVEGEIWV----------SSPSNASETFH 474
           GSV  E++           +VN ET      GV GEIWV            P   ++ F 
Sbjct: 401 GSVGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFD 460

Query: 475 SKLSN--KSSPN--FVRTGDRGVIKGADRYLFVTGRCSDVIKLQNNQEIHPHYIESVAYN 534
           +KL +   ++P   ++RTGD GVI  +D  LF+ GR  D++ + + +  +P  IE+    
Sbjct: 461 AKLVDPAPAAPEGPWLRTGDLGVI--SDGELFIMGRIKDLL-IVDGRNHYPDDIEATI-- 520

Query: 535 NFPAHLRGGCLAAF----KISETIAVVAEMQRFDKNDGESM---RRICEGIRKAVLIEEG 594
                + GG  AA      I+E +  + E +R      E M   R +   +  A+     
Sbjct: 521 ---QEITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHS 579

BLAST of Tan0012103 vs. NCBI nr
Match: XP_022962563.1 (uncharacterized protein LOC111462961 [Cucurbita moschata])

HSP 1 Score: 983.0 bits (2540), Expect = 1.2e-282
Identity = 519/652 (79.60%), Postives = 556/652 (85.28%), Query Frame = 0

Query: 1   MLFENFDPLFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADAGLNEEGSFLLTYKQL 60
           M +ENFDP+FPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTAD  LN EGSF LTY QL
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADV-LN-EGSF-LTYGQL 73

Query: 61  HDSVQFISDSLLRQLRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFGNENY 120
           HDSVQFISD L+RQ+RRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDP F NEN 
Sbjct: 74  HDSVQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENC 133

Query: 121 DHLARALSQTKPRAAIAHQAYIARVFRYLTSSPIDEKLASLLQSVQWISMEILKPCN--D 180
            HLARALSQTKPRAAIAHQAYIA VFRYL+ SP D KLA LLQSVQWISME L+ C   +
Sbjct: 134 HHLARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLQSVQWISMETLRTCTAAE 193

Query: 181 ANQKPSLISNNHQPNFH--PSSYHGCKPDEPYLIQYTSGATGVSKPVVVTAGAAVHNVRA 240
           A+Q  S+IS+  QP F+   SSY+GCKP+EPYLIQYTSGATG+ KPVVVTAGAA HNVRA
Sbjct: 194 ADQVVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRA 253

Query: 241 ARKSYDLNPNAVVVSWLPQYHDCGLMFLLLTVVTGATCVLTSPISFVTRPITWLHLITAF 300
           ARK+YDLNPN V+VSWLPQYHDCGLMFLLLTVV+GATCVLTSPISFVTRPITWLHLIT F
Sbjct: 254 ARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTF 313

Query: 301 RATCTPVPSFTLPLVLKRVKEDPVVNITGLDLGSLKNLILINEPIYRSAVEEFVEVFKAV 360
           +ATCTPVPSFTLPLVLKRVKE+      GLDL SL+NLILINEP+YRS VEEFV+VFKAV
Sbjct: 314 KATCTPVPSFTLPLVLKRVKEE-TPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAV 373

Query: 361 GLDPGCVSPSYGLAENCTFVSTAWSG--GGRRWWFPAMPSYRKLLPSARL-GEGSVEIEV 420
           GL+PGCVSPSYGLAENCTFVSTAWSG  GGRR WFPAMPSYRKLLPSARL  E S EIEV
Sbjct: 374 GLNPGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEV 433

Query: 421 VVVNAETREVVDDGVEGEIWVSSPSNAS----------ETFHSKLSNKSSPNFVRTGDRG 480
           VVVN ET E+V+DGVEGEIW+SSPSNAS          +TFH KLSNKSS  FVRTGDRG
Sbjct: 434 VVVNGETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKLSNKSSHKFVRTGDRG 493

Query: 481 VIKGADRYLFVTGRCSDVIKLQNNQEIHPHYIESVAYNNFPAHLRGGCLAAFKISETIAV 540
           VIKG DR+LFV GRCSDVI L NNQEIHPHYIES+AYNN  ++LRGGCLAA KIS+TIAV
Sbjct: 494 VIKGTDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAV 553

Query: 541 VAEMQRFDKNDGESMRRICEGIRKAVLIEEGIELGLVVLVKRGNVPKTTSGKVMRSAAKE 600
           VAEMQR DKND ES+R+ICEGIRKA LIEEGIELGLVVLVKRGNVPKTTSGKV R AAKE
Sbjct: 554 VAEMQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKE 613

Query: 601 RLAGGGMSVLMAVKFGKICGALKDLMKDSEGKGSVSDENEEFRSRPILLSLL 636
           +LAGGGMSVLMAVKFGK CG  KDL                  +RP+LLSLL
Sbjct: 614 KLAGGGMSVLMAVKFGKTCGVTKDLE----------------TNRPLLLSLL 645

BLAST of Tan0012103 vs. NCBI nr
Match: XP_023546350.1 (uncharacterized protein LOC111805474 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 982.2 bits (2538), Expect = 2.0e-282
Identity = 515/652 (78.99%), Postives = 556/652 (85.28%), Query Frame = 0

Query: 1   MLFENFDPLFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADAGLNEEGSFLLTYKQL 60
           M +ENFDP+FPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTAD  LN EGSF LTY QL
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADV-LN-EGSF-LTYGQL 73

Query: 61  HDSVQFISDSLLRQLRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFGNENY 120
           HDSVQFIS+ L+RQ+RRRDTV++LCSPGLDLVQLIYGCQRAGLVSVPISPPDPCF N+N 
Sbjct: 74  HDSVQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENKNC 133

Query: 121 DHLARALSQTKPRAAIAHQAYIARVFRYLTSSPIDEKLASLLQSVQWISMEILKPCN--D 180
            HLARALSQTKPRAAIAHQAYIA VFRYL+ SP D KLA LL+ VQWISME L+ C   +
Sbjct: 134 HHLARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLRGVQWISMETLRTCTAAE 193

Query: 181 ANQKPSLISNNHQPNFH--PSSYHGCKPDEPYLIQYTSGATGVSKPVVVTAGAAVHNVRA 240
           A+Q  S+IS+  QP F+   SSY+GCKP+EPYLIQYTSGATG+ KPVVVTAGAA HNVR 
Sbjct: 194 ADQVVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRV 253

Query: 241 ARKSYDLNPNAVVVSWLPQYHDCGLMFLLLTVVTGATCVLTSPISFVTRPITWLHLITAF 300
           ARK+YDLNPN V+VSWLPQYHDCGLMFLLLTVV+GATCVLTSPISFVTRPITWLHLIT F
Sbjct: 254 ARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTF 313

Query: 301 RATCTPVPSFTLPLVLKRVKEDPVVNITGLDLGSLKNLILINEPIYRSAVEEFVEVFKAV 360
           +ATCTPVPSFTLPLVLKRVKE+      GLDL SL+NLILINEP+YRS VEEFV+VFK V
Sbjct: 314 KATCTPVPSFTLPLVLKRVKEE-TPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKTV 373

Query: 361 GLDPGCVSPSYGLAENCTFVSTAWSG--GGRRWWFPAMPSYRKLLPSARL-GEGSVEIEV 420
           GL+PGCVSPSYGLAENCTFVSTAWSG  GGRR WFPAMPSYRKLLP A+L  E S EIEV
Sbjct: 374 GLNPGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPCAKLTDESSAEIEV 433

Query: 421 VVVNAETREVVDDGVEGEIWVSSPSNAS----------ETFHSKLSNKSSPNFVRTGDRG 480
           VVVN ET E+VDDGVEGEIW+SSPSNAS          +TFH KLSNKSS  FVRTGDRG
Sbjct: 434 VVVNGETGEIVDDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKLSNKSSHKFVRTGDRG 493

Query: 481 VIKGADRYLFVTGRCSDVIKLQNNQEIHPHYIESVAYNNFPAHLRGGCLAAFKISETIAV 540
           VIKG DR+LFV GRCSDVIKL NNQEIHPHYIES+AYNNF A+LRGGCLAA KIS+TIAV
Sbjct: 494 VIKGTDRFLFVIGRCSDVIKLNNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKISDTIAV 553

Query: 541 VAEMQRFDKNDGESMRRICEGIRKAVLIEEGIELGLVVLVKRGNVPKTTSGKVMRSAAKE 600
           VAEMQR DKND ES+R+ICEGIRKA LIEEGIELGLVVLVKRGNVPKTTSGKV R AAKE
Sbjct: 554 VAEMQRDDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKE 613

Query: 601 RLAGGGMSVLMAVKFGKICGALKDLMKDSEGKGSVSDENEEFRSRPILLSLL 636
           +LAGGGMSVLMAVKFG  CG  KDL    EG            +RP+LLSLL
Sbjct: 614 KLAGGGMSVLMAVKFGTTCGVTKDL----EG------------NRPLLLSLL 645

BLAST of Tan0012103 vs. NCBI nr
Match: KAG7029389.1 (fadD26, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 976.1 bits (2522), Expect = 1.5e-280
Identity = 516/652 (79.14%), Postives = 553/652 (84.82%), Query Frame = 0

Query: 1   MLFENFDPLFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADAGLNEEGSFLLTYKQL 60
           M +ENFDP+FPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTAD  LN EGSF LTY QL
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADV-LN-EGSF-LTYGQL 60

Query: 61  HDSVQFISDSLLRQLRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFGNENY 120
           HDSVQFISD L+RQ+RRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDP F NEN 
Sbjct: 61  HDSVQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENC 120

Query: 121 DHLARALSQTKPRAAIAHQAYIARVFRYLTSSPIDEKLASLLQSVQWISMEILKPCN--D 180
            HLARALSQTKP AAIAHQAYIA VFRYL+ SPID KLA LL+SVQWISME L+ C   +
Sbjct: 121 HHLARALSQTKPWAAIAHQAYIASVFRYLSLSPIDGKLALLLRSVQWISMESLRTCTAAE 180

Query: 181 ANQKPSLISNNHQPNFH--PSSYHGCKPDEPYLIQYTSGATGVSKPVVVTAGAAVHNVRA 240
           A+Q  S+IS   QP F+   SSY+GCKP+EPYLIQYTSGATG+ KPVVVTAGAA HNVR 
Sbjct: 181 ADQVVSVISRKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRV 240

Query: 241 ARKSYDLNPNAVVVSWLPQYHDCGLMFLLLTVVTGATCVLTSPISFVTRPITWLHLITAF 300
           ARK+YDLNPN V+VSWLPQYHDCGLMFLLLTVV+GATCVLTSPISFVTRPITWLHLIT F
Sbjct: 241 ARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTF 300

Query: 301 RATCTPVPSFTLPLVLKRVKEDPVVNITGLDLGSLKNLILINEPIYRSAVEEFVEVFKAV 360
           +ATCTPVPSFTLPLVLKRVKE+      GLDL SL+NLILINEP+YRS VEEFV+VFKAV
Sbjct: 301 KATCTPVPSFTLPLVLKRVKEE-TPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAV 360

Query: 361 GLDPGCVSPSYGLAENCTFVSTAWSG--GGRRWWFPAMPSYRKLLPSARL-GEGSVEIEV 420
           GL+PGCVSPSYGLAENCTFVSTAWSG  GGRR WFPAMPSYRKLLPSARL  E S EIEV
Sbjct: 361 GLNPGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEV 420

Query: 421 VVVNAETREVVDDGVEGEIWVSSPSNAS----------ETFHSKLSNKSSPNFVRTGDRG 480
           VVVN ET E+V+DGVEGEIW+SSPSNAS          +TFH KLSNKSS  FVRTGDRG
Sbjct: 421 VVVNGETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKLSNKSSHKFVRTGDRG 480

Query: 481 VIKGADRYLFVTGRCSDVIKLQNNQEIHPHYIESVAYNNFPAHLRGGCLAAFKISETIAV 540
           VIKG DR+LFV GRCSDVI L NNQEIHPHYIES+AYNN  ++LRGGCLAA KIS+TIAV
Sbjct: 481 VIKGTDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAV 540

Query: 541 VAEMQRFDKNDGESMRRICEGIRKAVLIEEGIELGLVVLVKRGNVPKTTSGKVMRSAAKE 600
           VAEMQR DKND ES+R+ICEGIRKA LIEEGIELGLVVLVKRGNVPKTTSGKV R AAKE
Sbjct: 541 VAEMQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKE 600

Query: 601 RLAGGGMSVLMAVKFGKICGALKDLMKDSEGKGSVSDENEEFRSRPILLSLL 636
           +LAGGGMSVLMAVKFGK CG  KDL                  +RP+LLS L
Sbjct: 601 KLAGGGMSVLMAVKFGKTCGITKDLE----------------TNRPLLLSFL 632

BLAST of Tan0012103 vs. NCBI nr
Match: KAG6598448.1 (hypothetical protein SDJN03_08226, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 966.1 bits (2496), Expect = 1.5e-277
Identity = 514/652 (78.83%), Postives = 551/652 (84.51%), Query Frame = 0

Query: 1   MLFENFDPLFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADAGLNEEGSFLLTYKQL 60
           M +ENFDP+FPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTAD  LN EGSF LTY QL
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADV-LN-EGSF-LTYGQL 60

Query: 61  HDSVQFISDSLLRQLRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFGNENY 120
           HDSVQFISD L+RQ+RRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDP F NEN 
Sbjct: 61  HDSVQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENC 120

Query: 121 DHLARALSQTKPRAAIAHQAYIARVFRYLTSSPIDEKLASLLQSVQWISMEILKPCN--D 180
            HLARALSQTKP AAIAHQAYIA VFRYL+ S ID KLA LL+SVQWISME L+ C   +
Sbjct: 121 HHLARALSQTKPWAAIAHQAYIASVFRYLSLSLIDGKLALLLRSVQWISMESLRTCTAAE 180

Query: 181 ANQKPSLISNNHQPNFH--PSSYHGCKPDEPYLIQYTSGATGVSKPVVVTAGAAVHNVRA 240
           A+Q  S+IS   QP F+   SSY+GCKP+EPYLIQYTSGATG+ KPVVVTAGAA HNVR 
Sbjct: 181 ADQVVSVISRKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRV 240

Query: 241 ARKSYDLNPNAVVVSWLPQYHDCGLMFLLLTVVTGATCVLTSPISFVTRPITWLHLITAF 300
           ARK+YDLNPN V+VSWLPQYHDCGLMFLLLTVV+GATCVLTSPISFVTRPITWLHLIT F
Sbjct: 241 ARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTF 300

Query: 301 RATCTPVPSFTLPLVLKRVKEDPVVNITGLDLGSLKNLILINEPIYRSAVEEFVEVFKAV 360
           +ATCTPVPSFTLPLVLKRVKE+      GLDL SL+NLILINEP+YRS VEEFV+VFKAV
Sbjct: 301 KATCTPVPSFTLPLVLKRVKEE-TPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAV 360

Query: 361 GLDPGCVSPSYGLAENCTFVSTAWSG--GGRRWWFPAMPSYRKLLPSARL-GEGSVEIEV 420
           GL+PGCVSPSYGLAENCTFVSTAWSG  GGRR WFPAMPSYRKLLPSARL  E S EIEV
Sbjct: 361 GLNPGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEV 420

Query: 421 VVVNAETREVVDDGVEGEIWVSSPSNAS----------ETFHSKLSNKSSPNFVRTGDRG 480
           VVVN ET E+V+DGVEGEIW+SSPSNAS          +TFH KLSNKSS  FVRTGDRG
Sbjct: 421 VVVNGETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKLSNKSSHKFVRTGDRG 480

Query: 481 VIKGADRYLFVTGRCSDVIKLQNNQEIHPHYIESVAYNNFPAHLRGGCLAAFKISETIAV 540
           VIKG DR+LFV GRCSDVI L NNQEIHPHYIES+AYNN  ++LRGGCLAA KIS+TIAV
Sbjct: 481 VIKGTDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAV 540

Query: 541 VAEMQRFDKNDGESMRRICEGIRKAVLIEEGIELGLVVLVKRGNVPKTTSGKVMRSAAKE 600
           VAEMQR DKND ES+R+ICEGIRKA LI EGIELGLVVLVKRGNVPKTTSGKV R AAKE
Sbjct: 541 VAEMQRQDKNDAESLRKICEGIRKAALI-EGIELGLVVLVKRGNVPKTTSGKVKRWAAKE 600

Query: 601 RLAGGGMSVLMAVKFGKICGALKDLMKDSEGKGSVSDENEEFRSRPILLSLL 636
           +LAGGGMSVLMAVKFGK CG  KDL                  +RP+LLS L
Sbjct: 601 KLAGGGMSVLMAVKFGKTCGVTKDLE----------------TNRPLLLSFL 631

BLAST of Tan0012103 vs. NCBI nr
Match: XP_022997536.1 (uncharacterized protein LOC111492429 [Cucurbita maxima])

HSP 1 Score: 963.4 bits (2489), Expect = 9.8e-277
Identity = 509/652 (78.07%), Postives = 551/652 (84.51%), Query Frame = 0

Query: 1   MLFENFDPLFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADAGLNEEGSFLLTYKQL 60
           M +ENFDP+FPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTAD  LN EGSF LTY QL
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADV-LN-EGSF-LTYGQL 60

Query: 61  HDSVQFISDSLLRQLRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFGNENY 120
           HDSVQFIS+ L+RQ+RRRDTV++LCSPGLDLVQLIYGCQRAGLVSVPISPPDPCF NEN 
Sbjct: 61  HDSVQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENC 120

Query: 121 DHLARALSQTKPRAAIAHQAYIARVFRYLTSSPIDEKLASLLQSVQWISMEILKPCN--D 180
            H ARALSQTKPRA IAH+ YI  VFRYL+SS ID KLA LLQSVQWISME L+ C   +
Sbjct: 121 HHFARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAE 180

Query: 181 ANQKPSLISNNHQPNFH--PSSYHGCKPDEPYLIQYTSGATGVSKPVVVTAGAAVHNVRA 240
           A+Q  S+IS+  +P F+   SSY+GCKP+EPYLIQYTSGATG+ KPVVVTAGAA HNVRA
Sbjct: 181 ADQVVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRA 240

Query: 241 ARKSYDLNPNAVVVSWLPQYHDCGLMFLLLTVVTGATCVLTSPISFVTRPITWLHLITAF 300
           ARK+YDLNPN V+VSWLPQYHDCGLMFLLLTVV+GATCVLTSPISFVTRPITWLHLIT F
Sbjct: 241 ARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTF 300

Query: 301 RATCTPVPSFTLPLVLKRVKEDPVVNITGLDLGSLKNLILINEPIYRSAVEEFVEVFKAV 360
           +ATCTPVPSFTLPLVLKRVKE+       LDL  L+NLILINEP+YRS VEEFV+VFKAV
Sbjct: 301 KATCTPVPSFTLPLVLKRVKEETPPG-GDLDLCCLRNLILINEPVYRSGVEEFVDVFKAV 360

Query: 361 GLDPGCVSPSYGLAENCTFVSTAWSG--GGRRWWFPAMPSYRKLLPSARLGEG-SVEIEV 420
           GL+PGCVSPSYGLAEN TFVSTAWSG  GG R  FPAMPSYRKLLP ARL +G S EIEV
Sbjct: 361 GLNPGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIEV 420

Query: 421 VVVNAETREVVDDGVEGEIWVSSPSNAS----------ETFHSKLSNKSSPNFVRTGDRG 480
           VVVN ET E+VDDGVEGEIW+SSPSNAS          +TFH KLSNKSS  FVRTGDRG
Sbjct: 421 VVVNGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRG 480

Query: 481 VIKGADRYLFVTGRCSDVIKLQNNQEIHPHYIESVAYNNFPAHLRGGCLAAFKISETIAV 540
           VIKGADR+LFV GRCSDVI L NNQEIHPHYIES+AYNNF A+LRGGCLAA K+S+TIAV
Sbjct: 481 VIKGADRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDTIAV 540

Query: 541 VAEMQRFDKNDGESMRRICEGIRKAVLIEEGIELGLVVLVKRGNVPKTTSGKVMRSAAKE 600
           VAEMQR DKND E++R+ICEGIRKA LIEEGIELGLVVLVKRGNVPKTTSGKV R  AKE
Sbjct: 541 VAEMQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKE 600

Query: 601 RLAGGGMSVLMAVKFGKICGALKDLMKDSEGKGSVSDENEEFRSRPILLSLL 636
           +LAGGGMSVLMAVKFG ICG  KDL           D N     RP+LLSLL
Sbjct: 601 KLAGGGMSVLMAVKFGTICGVTKDL-----------DGN-----RPLLLSLL 632

BLAST of Tan0012103 vs. ExPASy TrEMBL
Match: A0A6J1HF51 (uncharacterized protein LOC111462961 OS=Cucurbita moschata OX=3662 GN=LOC111462961 PE=4 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 5.8e-283
Identity = 519/652 (79.60%), Postives = 556/652 (85.28%), Query Frame = 0

Query: 1   MLFENFDPLFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADAGLNEEGSFLLTYKQL 60
           M +ENFDP+FPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTAD  LN EGSF LTY QL
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADV-LN-EGSF-LTYGQL 73

Query: 61  HDSVQFISDSLLRQLRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFGNENY 120
           HDSVQFISD L+RQ+RRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDP F NEN 
Sbjct: 74  HDSVQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENC 133

Query: 121 DHLARALSQTKPRAAIAHQAYIARVFRYLTSSPIDEKLASLLQSVQWISMEILKPCN--D 180
            HLARALSQTKPRAAIAHQAYIA VFRYL+ SP D KLA LLQSVQWISME L+ C   +
Sbjct: 134 HHLARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLQSVQWISMETLRTCTAAE 193

Query: 181 ANQKPSLISNNHQPNFH--PSSYHGCKPDEPYLIQYTSGATGVSKPVVVTAGAAVHNVRA 240
           A+Q  S+IS+  QP F+   SSY+GCKP+EPYLIQYTSGATG+ KPVVVTAGAA HNVRA
Sbjct: 194 ADQVVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRA 253

Query: 241 ARKSYDLNPNAVVVSWLPQYHDCGLMFLLLTVVTGATCVLTSPISFVTRPITWLHLITAF 300
           ARK+YDLNPN V+VSWLPQYHDCGLMFLLLTVV+GATCVLTSPISFVTRPITWLHLIT F
Sbjct: 254 ARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTF 313

Query: 301 RATCTPVPSFTLPLVLKRVKEDPVVNITGLDLGSLKNLILINEPIYRSAVEEFVEVFKAV 360
           +ATCTPVPSFTLPLVLKRVKE+      GLDL SL+NLILINEP+YRS VEEFV+VFKAV
Sbjct: 314 KATCTPVPSFTLPLVLKRVKEE-TPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAV 373

Query: 361 GLDPGCVSPSYGLAENCTFVSTAWSG--GGRRWWFPAMPSYRKLLPSARL-GEGSVEIEV 420
           GL+PGCVSPSYGLAENCTFVSTAWSG  GGRR WFPAMPSYRKLLPSARL  E S EIEV
Sbjct: 374 GLNPGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEV 433

Query: 421 VVVNAETREVVDDGVEGEIWVSSPSNAS----------ETFHSKLSNKSSPNFVRTGDRG 480
           VVVN ET E+V+DGVEGEIW+SSPSNAS          +TFH KLSNKSS  FVRTGDRG
Sbjct: 434 VVVNGETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKLSNKSSHKFVRTGDRG 493

Query: 481 VIKGADRYLFVTGRCSDVIKLQNNQEIHPHYIESVAYNNFPAHLRGGCLAAFKISETIAV 540
           VIKG DR+LFV GRCSDVI L NNQEIHPHYIES+AYNN  ++LRGGCLAA KIS+TIAV
Sbjct: 494 VIKGTDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAV 553

Query: 541 VAEMQRFDKNDGESMRRICEGIRKAVLIEEGIELGLVVLVKRGNVPKTTSGKVMRSAAKE 600
           VAEMQR DKND ES+R+ICEGIRKA LIEEGIELGLVVLVKRGNVPKTTSGKV R AAKE
Sbjct: 554 VAEMQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKE 613

Query: 601 RLAGGGMSVLMAVKFGKICGALKDLMKDSEGKGSVSDENEEFRSRPILLSLL 636
           +LAGGGMSVLMAVKFGK CG  KDL                  +RP+LLSLL
Sbjct: 614 KLAGGGMSVLMAVKFGKTCGVTKDLE----------------TNRPLLLSLL 645

BLAST of Tan0012103 vs. ExPASy TrEMBL
Match: A0A6J1K7R6 (uncharacterized protein LOC111492429 OS=Cucurbita maxima OX=3661 GN=LOC111492429 PE=4 SV=1)

HSP 1 Score: 963.4 bits (2489), Expect = 4.7e-277
Identity = 509/652 (78.07%), Postives = 551/652 (84.51%), Query Frame = 0

Query: 1   MLFENFDPLFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADAGLNEEGSFLLTYKQL 60
           M +ENFDP+FPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTAD  LN EGSF LTY QL
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADV-LN-EGSF-LTYGQL 60

Query: 61  HDSVQFISDSLLRQLRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFGNENY 120
           HDSVQFIS+ L+RQ+RRRDTV++LCSPGLDLVQLIYGCQRAGLVSVPISPPDPCF NEN 
Sbjct: 61  HDSVQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENC 120

Query: 121 DHLARALSQTKPRAAIAHQAYIARVFRYLTSSPIDEKLASLLQSVQWISMEILKPCN--D 180
            H ARALSQTKPRA IAH+ YI  VFRYL+SS ID KLA LLQSVQWISME L+ C   +
Sbjct: 121 HHFARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAE 180

Query: 181 ANQKPSLISNNHQPNFH--PSSYHGCKPDEPYLIQYTSGATGVSKPVVVTAGAAVHNVRA 240
           A+Q  S+IS+  +P F+   SSY+GCKP+EPYLIQYTSGATG+ KPVVVTAGAA HNVRA
Sbjct: 181 ADQVVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRA 240

Query: 241 ARKSYDLNPNAVVVSWLPQYHDCGLMFLLLTVVTGATCVLTSPISFVTRPITWLHLITAF 300
           ARK+YDLNPN V+VSWLPQYHDCGLMFLLLTVV+GATCVLTSPISFVTRPITWLHLIT F
Sbjct: 241 ARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTF 300

Query: 301 RATCTPVPSFTLPLVLKRVKEDPVVNITGLDLGSLKNLILINEPIYRSAVEEFVEVFKAV 360
           +ATCTPVPSFTLPLVLKRVKE+       LDL  L+NLILINEP+YRS VEEFV+VFKAV
Sbjct: 301 KATCTPVPSFTLPLVLKRVKEETPPG-GDLDLCCLRNLILINEPVYRSGVEEFVDVFKAV 360

Query: 361 GLDPGCVSPSYGLAENCTFVSTAWSG--GGRRWWFPAMPSYRKLLPSARLGEG-SVEIEV 420
           GL+PGCVSPSYGLAEN TFVSTAWSG  GG R  FPAMPSYRKLLP ARL +G S EIEV
Sbjct: 361 GLNPGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIEV 420

Query: 421 VVVNAETREVVDDGVEGEIWVSSPSNAS----------ETFHSKLSNKSSPNFVRTGDRG 480
           VVVN ET E+VDDGVEGEIW+SSPSNAS          +TFH KLSNKSS  FVRTGDRG
Sbjct: 421 VVVNGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRG 480

Query: 481 VIKGADRYLFVTGRCSDVIKLQNNQEIHPHYIESVAYNNFPAHLRGGCLAAFKISETIAV 540
           VIKGADR+LFV GRCSDVI L NNQEIHPHYIES+AYNNF A+LRGGCLAA K+S+TIAV
Sbjct: 481 VIKGADRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDTIAV 540

Query: 541 VAEMQRFDKNDGESMRRICEGIRKAVLIEEGIELGLVVLVKRGNVPKTTSGKVMRSAAKE 600
           VAEMQR DKND E++R+ICEGIRKA LIEEGIELGLVVLVKRGNVPKTTSGKV R  AKE
Sbjct: 541 VAEMQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKE 600

Query: 601 RLAGGGMSVLMAVKFGKICGALKDLMKDSEGKGSVSDENEEFRSRPILLSLL 636
           +LAGGGMSVLMAVKFG ICG  KDL           D N     RP+LLSLL
Sbjct: 601 KLAGGGMSVLMAVKFGTICGVTKDL-----------DGN-----RPLLLSLL 632

BLAST of Tan0012103 vs. ExPASy TrEMBL
Match: A0A0A0LL23 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361690 PE=4 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 7.1e-257
Identity = 476/648 (73.46%), Postives = 528/648 (81.48%), Query Frame = 0

Query: 1   MLFENFDPLFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADAGLNEEGSFLLTYKQL 60
           ML+ENFDPLFP+QPVVD YLPVWASLPAF+SKPAFIWSEDGTA A +NE+    LTY+QL
Sbjct: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFQSKPAFIWSEDGTAKA-INEDS--FLTYRQL 60

Query: 61  HDSVQFISDSLLRQLRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFGNENY 120
           HDSVQ I++ LLRQLRRRDTVV+LCS GLD VQLIYGCQRAGLVSVPISPPD    NEN 
Sbjct: 61  HDSVQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENC 120

Query: 121 DHLARALSQTKPRAAIAHQAYIARVFRYLTSSPIDEKLASLLQSVQWISMEILKPCNDAN 180
            HLARALSQTKPRAAIAHQ+YI  VFRY++SS  D+KLA LL+ V+WISME LK     +
Sbjct: 121 HHLARALSQTKPRAAIAHQSYINTVFRYISSS-ADKKLALLLKGVRWISMESLK---QPH 180

Query: 181 QKPSLISNNHQPNF-HPSSYHGCKPDEPYLIQYTSGATGVSKPVVVTAGAAVHNVRAARK 240
           ++  L  + HQ +F H SSY GC PD+PYLIQYTSGAT +SK VV+TAGAA HNVRAARK
Sbjct: 181 KESELNQHKHQSSFYHSSSYSGCNPDDPYLIQYTSGATAISKAVVITAGAAAHNVRAARK 240

Query: 241 SYDLNPNAVVVSWLPQYHDCGLMFLLLTVVTGATCVLTSPISFVTRPITWLHLITAFRAT 300
           +YDLNPN ++VSWLPQYHDCGLMFLLLTV+TGATCVLTSPISFVT+PITWLHLITAF+AT
Sbjct: 241 AYDLNPNDLIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKAT 300

Query: 301 CTPVPSFTLPLVLKRVKEDPVVNITGLDLGSLKNLILINEPIYRSAVEEFVEVFKAVGLD 360
           CTPVPSFTLPLVLKRV          LDL SL+NLILINEPIYRS VEEFVEVFK VGLD
Sbjct: 301 CTPVPSFTLPLVLKRVGYSS-GRAGNLDLRSLRNLILINEPIYRSTVEEFVEVFKTVGLD 360

Query: 361 PGCVSPSYGLAENCTFVSTAWSGGGRRWWFPAMPSYRKLLPSARLGEGSV-EIEVVVVNA 420
           PGCVSPSYGLAENCTFVSTAW GGG    FPAMPSYR+LLPS RL +G   EIEVVVVN 
Sbjct: 361 PGCVSPSYGLAENCTFVSTAWCGGG---GFPAMPSYRQLLPSGRLRDGMCKEIEVVVVNE 420

Query: 421 ETREVVDDGVEGEIWVSSPSNA----------SETFHSKLSNKSSPNFVRTGDRGVIKGA 480
           ET EVV+DGVEGEIWVSSPSNA           ETFHSKL NKSS NFVRTGDRGVIKG+
Sbjct: 421 ETGEVVEDGVEGEIWVSSPSNALGYLGHPSVTEETFHSKLKNKSSLNFVRTGDRGVIKGS 480

Query: 481 DRYLFVTGRCSDVIKL-QNNQEIHPHYIESVAYNNFPAHLRGGCLAAFKISETIAVVAEM 540
           DR+LFV GRCSDVIKL  NNQ+IHPHYIES +YNNF A+LRGGC+AA K+S TIA+VAEM
Sbjct: 481 DRFLFVIGRCSDVIKLNNNNQQIHPHYIESTSYNNFSAYLRGGCIAAVKVSGTIALVAEM 540

Query: 541 QRFDKNDGESMRRICEGIRKAVLIEEGIELGLVVLVKRGNVPKTTSGKVMRSAAKERLAG 600
           QR D++D E +R+ICE IRKAVLIEEGIELGLVVLVKRGNV KTTSGKV R   KE+LAG
Sbjct: 541 QRDDRHDAELLRKICEDIRKAVLIEEGIELGLVVLVKRGNVSKTTSGKVKRWVVKEKLAG 600

Query: 601 GGMSVLMAVKFGKICGALKDLMKDSEGKGSVSDENEEFRSRPILLSLL 636
           GG+ VLMA +FGK C  LK                 EF +RP+L+S+L
Sbjct: 601 GGLGVLMAFEFGKNCVDLK--------------RKGEFETRPVLISML 623

BLAST of Tan0012103 vs. ExPASy TrEMBL
Match: A0A498HUN9 (AMP-binding domain-containing protein OS=Malus domestica OX=3750 GN=DVH24_012901 PE=4 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 1.5e-206
Identity = 375/635 (59.06%), Postives = 473/635 (74.49%), Query Frame = 0

Query: 1   MLFENFDPLFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADAGLNEEGSFLLTYKQL 60
           M +EN+DP +PDQPVVDLY PVWASLPAFRSKPAF+W EDG+A+     E +  LTY Q+
Sbjct: 2   MNYENYDPSYPDQPVVDLYFPVWASLPAFRSKPAFVWVEDGSAN-----EATSTLTYGQM 61

Query: 61  HDSVQFISDSLLRQLRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFGNENY 120
           +DSVQ I+  LL  L+R D VVILCSPGL+LV+ I+GCQRAGL+SVP+SPPDP F N+N+
Sbjct: 62  NDSVQCIASQLLIPLQRGDAVVILCSPGLELVETIFGCQRAGLLSVPVSPPDPSFTNQNF 121

Query: 121 DHLARALSQTKPRAAIAHQAYIARVFRYLTSSPIDEKLASLLQSVQWISMEILKPCNDAN 180
            HL RALSQTKP+AA+AH  YI R+  Y++    D+ L   L++++WI+++ +K  +   
Sbjct: 122 HHLIRALSQTKPKAALAHSTYITRIQHYISLPSSDKDLVQRLKNLRWIAIDDIKTKDTTL 181

Query: 181 QKPSLISNNHQPNFHPSSYHGCKPDEPYLIQYTSGATGVSKPVVVTAGAAVHNVRAARKS 240
           Q        H P      Y+GC+ ++ YL+QYTSGATG+ KPV+VTAG+A HNVR ARK+
Sbjct: 182 Q--------HSP------YNGCRAEDLYLVQYTSGATGIPKPVLVTAGSAAHNVRTARKA 241

Query: 241 YDLNPNAVVVSWLPQYHDCGLMFLLLTVVTGATCVLTSPISFVTRPITWLHLITAFRATC 300
           YDL+PN+V+VSWLPQYHDCGLMFLLLT+V+GATCVLTSP +FV RP  WL LI+ F ATC
Sbjct: 242 YDLHPNSVIVSWLPQYHDCGLMFLLLTIVSGATCVLTSPGAFVNRPRLWLELISNFNATC 301

Query: 301 TPVPSFTLPLVLKRVKEDPVVNITGLDLGSLKNLILINEPIYRSAVEEFVEVFKAVGLDP 360
           TPVPSFTLPLV+KR   D     + ++L S+KNLI+INEPIYR AVE+FV+VFK  GL+P
Sbjct: 302 TPVPSFTLPLVVKRGGVDK--GTSPINLWSMKNLIIINEPIYRDAVEQFVDVFKPYGLNP 361

Query: 361 GCVSPSYGLAENCTFVSTAWSGGGRRWWFPAMPSYRKLLPSARLGEGSVEIE---VVVVN 420
             +SPSYGLAENCTFVSTAW           +PS+ KLLPSARL E   ++E   +VVVN
Sbjct: 362 SSISPSYGLAENCTFVSTAWRSD------TPLPSHNKLLPSARLNENDDQLEDMNIVVVN 421

Query: 421 AETREVVDDGVEGEIWVSSPSNAS----------ETFHSKLSNKSSPNFVRTGDRGVIKG 480
            ET E V+DG+EGEIWVSSPSNAS          E ++ +L NK S  F+RTGDRGV+KG
Sbjct: 422 EETHEAVEDGIEGEIWVSSPSNASGYLAHPNLTREVYYGRLKNKLSRPFLRTGDRGVVKG 481

Query: 481 ADRYLFVTGRCSDVIKLQNN---QEIHPHYIESVAYNNFPAHLRGGCLAAFKISETIAVV 540
            DRYLFV GRC DVI+L N+    EIHPHY+E+VAYN+ P  LRGGCLAAF++S T+ VV
Sbjct: 482 EDRYLFVMGRCVDVIRLPNDDVGMEIHPHYVETVAYNSNPGFLRGGCLAAFEVSNTVVVV 541

Query: 541 AEMQRFDKNDGESMRRICEGIRKAVLIEEGIELGLVVLVKRGNVPKTTSGKVMRSAAKER 600
           AE QR +KN G  +R+IC+G+R  V++EEG+E+G VVLV+ G VPKTTSGK+ R AAK++
Sbjct: 542 AETQRMEKNIG-VLRKICDGVRNGVVMEEGVEVGTVVLVRSGCVPKTTSGKIQRWAAKDK 601

Query: 601 LAGGGMSVLMAVKFGKICGALKDL---MKDSEGKG 617
           L GG M VLM V+FG   G++  L   + +SEG+G
Sbjct: 602 LVGGKMGVLMEVRFGDYGGSVSSLGAEVGESEGRG 608

BLAST of Tan0012103 vs. ExPASy TrEMBL
Match: A0A5N5GWT5 (Disco-interacting protein 2 B-A-like OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_015476 PE=4 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 2.0e-206
Identity = 375/635 (59.06%), Postives = 474/635 (74.65%), Query Frame = 0

Query: 1   MLFENFDPLFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADAGLNEEGSFLLTYKQL 60
           M +EN+DP +PDQPVVDLYLPVWASLPAFRSKPAF+W +DG+A+     E +  LT+ QL
Sbjct: 2   MDYENYDPSYPDQPVVDLYLPVWASLPAFRSKPAFVWVDDGSAN-----EATSTLTFGQL 61

Query: 61  HDSVQFISDSLLRQLRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFGNENY 120
           +DSVQ I+  LL  L+R D VVILCSPGL+LV+ I+GCQRAGL+SVP+SPPDP F N N+
Sbjct: 62  NDSVQSIASQLLTPLQRGDAVVILCSPGLELVETIFGCQRAGLLSVPVSPPDPSFTNPNF 121

Query: 121 DHLARALSQTKPRAAIAHQAYIARVFRYLTSSPIDEKLASLLQSVQWISMEILKPCNDAN 180
            HL RALSQTKP+AA+AH  YI R+  Y++    D+ L   LQ+++WI+++ +K  +   
Sbjct: 122 HHLIRALSQTKPKAALAHSTYITRIQHYISLPSSDKDLVQRLQNLRWIAIDDIKTKDTTL 181

Query: 181 QKPSLISNNHQPNFHPSSYHGCKPDEPYLIQYTSGATGVSKPVVVTAGAAVHNVRAARKS 240
           Q        H P      Y GC+ ++ YL+QYTSGATG+ KPV+VTAG+A HNVR ARK+
Sbjct: 182 Q--------HSP------YKGCRAEDLYLVQYTSGATGIPKPVLVTAGSAAHNVRTARKA 241

Query: 241 YDLNPNAVVVSWLPQYHDCGLMFLLLTVVTGATCVLTSPISFVTRPITWLHLITAFRATC 300
           YDL+PN+V+VSWLPQYHDCGLMFLLLT+V+GATCVLTSP +FV RP  WL LI+ F ATC
Sbjct: 242 YDLHPNSVIVSWLPQYHDCGLMFLLLTIVSGATCVLTSPGAFVNRPRLWLELISNFNATC 301

Query: 301 TPVPSFTLPLVLKRVKEDPVVNITGLDLGSLKNLILINEPIYRSAVEEFVEVFKAVGLDP 360
           TPVPSFTLPLV+KR   D     + ++L S+KNLI+INEPIYR AVE+FV+VFK  GL+P
Sbjct: 302 TPVPSFTLPLVVKRGGVDK--GTSPINLWSMKNLIIINEPIYRDAVEQFVDVFKPYGLNP 361

Query: 361 GCVSPSYGLAENCTFVSTAWSGGGRRWWFPAMPSYRKLLPSARLGEGSVEIE---VVVVN 420
             +SPSYGLAENCTFVSTAW           +PS+ KLLPSARL E   ++E   +VVVN
Sbjct: 362 SSISPSYGLAENCTFVSTAWRSD------TPLPSHNKLLPSARLNENDDQLEDMNIVVVN 421

Query: 421 AETREVVDDGVEGEIWVSSPSNAS----------ETFHSKLSNKSSPNFVRTGDRGVIKG 480
            ET E V+DG+EGEIWVSSPSNAS          E ++ +L NK S  F+RTGDRGV+KG
Sbjct: 422 EETHEAVEDGIEGEIWVSSPSNASGYLAHPNLTREVYYGRLKNKLSRPFLRTGDRGVVKG 481

Query: 481 ADRYLFVTGRCSDVIKLQNN---QEIHPHYIESVAYNNFPAHLRGGCLAAFKISETIAVV 540
            DRYLFVTGRC+DVI+L+N+    EIHPHY+E+ AYN+ P  LRGGCLAAF++S T+ VV
Sbjct: 482 EDRYLFVTGRCADVIRLRNDDVGMEIHPHYVETAAYNSNPGFLRGGCLAAFEVSNTVVVV 541

Query: 541 AEMQRFDKNDGESMRRICEGIRKAVLIEEGIELGLVVLVKRGNVPKTTSGKVMRSAAKER 600
           AE QR +K+ G  +R+IC+G+R  V++EEG+E+G VVLV+ G VPKTTSGK+ R AAK++
Sbjct: 542 AETQRMEKDIG-VLRKICDGVRHGVVMEEGVEVGTVVLVRSGCVPKTTSGKIQRWAAKDK 601

Query: 601 LAGGGMSVLMAVKFGKICGALKDL---MKDSEGKG 617
           L GG M VLM V+FG   G++  L   + +SEG+G
Sbjct: 602 LVGGKMGVLMEVRFGDYGGSVPSLGAGVGESEGRG 608