Tan0012019 (gene) Snake gourd v1

BLAST of Tan0012019 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 8.4e-81
Identity = 152/345 (44.06%), Postives = 215/345 (62.32%), Query Frame = 0

Query: 61  VFDVKDYGAVADGKTENSLAFMAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFDDLS 120
           VF+V DYG  A G  + S A M AW+ ACA +G ST+LIP+GN+ +GEV+  GPC     
Sbjct: 9   VFNVNDYG--AKGAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSKI 68

Query: 121 PKVL--ILGTLVAPTTLTADVWIRFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCRQRM 180
              +  ++     P+   +D W+ F  +  LT++G      LDGQG   W   ++C +  
Sbjct: 69  GFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSG---TGTLDGQGQTAWA-KNNCDKNP 128

Query: 181 TCDIFTTSLKLTDVSNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGIH 240
            C     +L+   + + +V +I+ +NSK FH+ + +C++I      +TAP  S NTDGIH
Sbjct: 129 NCKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIH 188

Query: 241 ISQSTNINVTASDIGVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMGI 300
           +  S  + +T + I  GDDCISIGPGS N++++ + CGPGHGIS+GSLGRY  EK+V GI
Sbjct: 189 VGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGI 248

Query: 301 RVENCTINGTQNGVRVKTWPGDQASNATNLTFQNIVMFNVSNPIIIDQQYCPNHSCDTQE 360
            V+ CT +GT NGVRVKTWP      AT+LTFQ++ M NV NP+I+DQ+YCP   C  Q 
Sbjct: 249 TVKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQA 308

Query: 361 SSSVKLSDINIRNISGTYNSEFAVDLQCSSALPCENIQLSYINLT 404
            S +KLS+IN  NI GT   + AV + CS  +PC N+++  INL+
Sbjct: 309 PSRIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLS 347

BLAST of Tan0012019 vs. ExPASy Swiss-Prot
Match: P49063 (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 298.9 bits (764), Expect = 9.3e-80
Identity = 172/411 (41.85%), Postives = 236/411 (57.42%), Query Frame = 0

Query: 2   KLSALLFSFLLAVSVSARNFSP--DPALESAAAATADAWDFLLPPLDMAEPPAGDPWRGL 61
           K S +L   L+ ++ +  N +P  D A ++AA A  D        +  A    G    G 
Sbjct: 6   KASGVLLVLLVVMATTIANGTPVVDKA-KNAATAVEDTAKNAATAVGGAAASVGAKVSGA 65

Query: 62  R---VFDVKDYGAVADGKTENSLAFMAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCF 121
           +     DVK  GA  D KT++S AF AAW++ACA    ST+ +P+G + V  + F GPC 
Sbjct: 66  KPGAAVDVKASGAKGDSKTDDSAAFAAAWKEACA--AGSTITVPKGEYMVESLEFKGPC- 125

Query: 122 DDLSPKVLIL-GTLVAPTTLTADV----WIRFDSLRHLTLTGGHQPAILDGQGADTWTYG 181
               P  L L G   AP T+        WI F+++   TL G    AI DGQG+  W   
Sbjct: 126 --KGPVTLELNGNFKAPATVKTTKPHAGWIDFENIADFTLNG--NKAIFDGQGSLAWK-A 185

Query: 182 SDCRQRMTCDIFTTSLKLTDVSNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWDS 241
           +DC +   C+    +++ T ++N  +++I+  NSK FHM + +C NI LS   I AP +S
Sbjct: 186 NDCAKTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPES 245

Query: 242 QNTDGIHISQSTNINVTASDIGVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYKE 301
            NTDGIHI +S  +N+  + I  GDDC+SIG G+ N+ V ++ CGPGHGIS+GSLGRY  
Sbjct: 246 LNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPN 305

Query: 302 EKDVMGIRVENCTINGTQNGVRVKTWPGDQASNATNLTFQNIVMFNVSNPIIIDQQYCPN 361
           E+ V G+ V  C I  T NGVR+KTWPG     A+N+ F++I M NVS P++IDQ+YCP 
Sbjct: 306 EQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPY 365

Query: 362 HSCDTQESSSVKLSDINIRNISGTYNSEFAVDLQCSSALPCENIQLSYINL 403
             C     S VKLSD+ I+ I GT  ++ AV L CS  +PC NI LS INL
Sbjct: 366 GHCKAGVPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINL 407

BLAST of Tan0012019 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 5.1e-78
Identity = 150/347 (43.23%), Postives = 222/347 (63.98%), Query Frame = 0

Query: 62  FDVKDYGAVADGKTENSLAFMAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFDDLSP 121
           FD+   GA  +GKT+++ A   AW  AC   G  T+LIP+G+F VG ++F+GPC  D++ 
Sbjct: 41  FDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGPLNFTGPCKGDVT- 100

Query: 122 KVLILGTLVAPTTLTA----DVWIRFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCRQR 181
            + + G L+A T L+       WI    + +L +TG  +   LDGQG   W+  S C ++
Sbjct: 101 -IQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGK---LDGQGPAVWSKNS-CVKK 160

Query: 182 MTCDIFTTSLKLTDVSNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGI 241
             C I   SL +  V+NG VS I+L+NSK FHM +Y C ++ +   N+TAP DS NTDGI
Sbjct: 161 YDCKILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGI 220

Query: 242 HISQSTNINVTASDIGVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMG 301
           H+  S+ + +T + IGVGDDCISIGPG+  ++++ + CGPGHGIS+GSLGRYK+EKDV  
Sbjct: 221 HMGDSSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTD 280

Query: 302 IRVENCTINGTQNGVRVKTWPGDQAS--NATNLTFQNIVMFNVSNPIIIDQQYCPNHSCD 361
           I V++CT+  T NGVR+K +  D AS   A+ + ++NI M +   PIIID +YCPN  C 
Sbjct: 281 INVKDCTLKKTANGVRIKAYE-DAASVLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCT 340

Query: 362 TQESSSVKLSDINIRNISGTYNSEFAVDLQCSSALPCENIQLSYINL 403
              +S V + D+  +NI+GT ++  AV+L C++ +PC  + +  +N+
Sbjct: 341 ANGASKVTVKDVTFKNITGTSSTPEAVNLLCTAKIPCTGVTMDDVNI 380

BLAST of Tan0012019 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 7.3e-77
Identity = 144/331 (43.50%), Postives = 210/331 (63.44%), Query Frame = 0

Query: 76  ENSLAFMAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFDDLSPKVLILGTLVA---P 135
           +++ A   AW++ACA    ST+L+P+GNF VG ++  GPC   +   + + GTL A   P
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIG--LQLQGTLKAPADP 60

Query: 136 TTLTADVWIRFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCRQRMTCDIFTTSLKLTDV 195
           + +    WI  + +  LT+ GG    + DGQG   W      +    C   + +L+L  V
Sbjct: 61  SKIKGLGWINLNKIDLLTIFGG---GVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAV 120

Query: 196 SNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGIHISQSTNINVTASDI 255
           +N I+ +++ ++SK FH+ +  C N+    F I+A   S NTDGIHI +S  +N+  ++I
Sbjct: 121 TNSILRDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEI 180

Query: 256 GVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMGIRVENCTINGTQNGV 315
             GDDCIS+G GS NI++++I CGPGHGISVGSLGRYK E+ V+GI V+NCTI G+QNGV
Sbjct: 181 KTGDDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGV 240

Query: 316 RVKTWPGDQASNATNLTFQNIVMFNVSNPIIIDQQYCPNHSCDTQESSSVKLSDINIRNI 375
           R+KTWP  +   A+ + FQ+I M +V  PI+IDQ YCP + C  +  SSVKLS I+ +NI
Sbjct: 241 RIKTWPKSEPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNI 300

Query: 376 SGTYNSEFAVDLQCSSALPCENIQLSYINLT 404
            GT  ++ AV L CS + PC  ++L+ I+LT
Sbjct: 301 KGTSTTKEAVKLVCSKSFPCNGVELADIDLT 326

BLAST of Tan0012019 vs. ExPASy Swiss-Prot
Match: P35338 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 9.6e-77
Identity = 147/347 (42.36%), Postives = 218/347 (62.82%), Query Frame = 0

Query: 62  FDVKDYGAVADGKTENSLAFMAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFDDLSP 121
           FD+   GA  +GKT+++ A   AW  AC   G  T+LIP+G+F VG+++F+GPC  D++ 
Sbjct: 41  FDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVT- 100

Query: 122 KVLILGTLVAPTTLTA----DVWIRFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCRQR 181
            + + G L+A T L+       WI    + +L +TG      LDGQG   W+  S C ++
Sbjct: 101 -IQVDGNLLATTDLSQYKEHGNWIEILRVDNLVITG---KGNLDGQGPAVWSKNS-CTKK 160

Query: 182 MTCDIFTTSLKLTDVSNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGI 241
             C I   SL +  V+NG VS ++L+NSK FHM +Y C N+ +    +TAP DS NTDGI
Sbjct: 161 YDCKILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYQCKNMLIKDVTVTAPGDSPNTDGI 220

Query: 242 HISQSTNINVTASDIGVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMG 301
           H+  S+ I +T + IGVGDDCISIGPG+  ++++ + CGPGHGIS+GSLGRYK+EKDV  
Sbjct: 221 HMGDSSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTD 280

Query: 302 IRVENCTINGTQNGVRVKTWPGDQAS--NATNLTFQNIVMFNVSNPIIIDQQYCPNHSCD 361
           I V++CT+  T  GVR+K +  D AS    + + ++NI M + +NPI ID +YCPN  C 
Sbjct: 281 INVKDCTLKKTMFGVRIKAYE-DAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCT 340

Query: 362 TQESSSVKLSDINIRNISGTYNSEFAVDLQCSSALPCENIQLSYINL 403
              +S V + D+  +NI+GT ++  A+ L C++ +PC    +  +N+
Sbjct: 341 ANGASKVTVKDVTFKNITGTSSTPEAISLLCTAKVPCTGATMDDVNV 380

BLAST of Tan0012019 vs. NCBI nr
Match: XP_023539060.1 (exopolygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 735.3 bits (1897), Expect = 3.0e-208
Identity = 360/434 (82.95%), Postives = 392/434 (90.32%), Query Frame = 0

Query: 1   MKLSAL-LFSFLLAVSVSARNFSPDPALESAAAATADAWDFLLPPLDMAEPPAGDPWRGL 60
           MKLSAL +F FLLAVSVS RNFSP+PALESAA     +WDFLL P DMAEPP GDPWRGL
Sbjct: 1   MKLSALIIFYFLLAVSVSGRNFSPEPALESAAG----SWDFLLLPPDMAEPPVGDPWRGL 60

Query: 61  RVFDVKDYGAVADGKTENSLAFMAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFDDL 120
            VFDV DYGA+ADGKTENSLAFM AWEDACAYRGSSTLLIPEG+FF+ +V FSGPCF DL
Sbjct: 61  AVFDVTDYGAIADGKTENSLAFMGAWEDACAYRGSSTLLIPEGSFFLAQVLFSGPCFGDL 120

Query: 121 SPKVLILGTLVAPTTLTADVWIRFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCRQRMT 180
           SPKV+ILGTL+APTTLTADVWI F SLRHLT+TG     ILDGQGA+TW YG +CR R++
Sbjct: 121 SPKVIILGTLIAPTTLTADVWIYFKSLRHLTITGNVGSGILDGQGAETWLYGFNCRHRLS 180

Query: 181 CDIFTTSLKLTDVSNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGIHI 240
           C IFTTSLKL+DVSN  VSNISLVNSKGFH+AL  CDNIHLSGFNITAPW+SQNTDGIHI
Sbjct: 181 CGIFTTSLKLSDVSNATVSNISLVNSKGFHVALNGCDNIHLSGFNITAPWNSQNTDGIHI 240

Query: 241 SQSTNINVTASDIGVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMGIR 300
           SQSTNIN+T SDI VGDDC+SIGPGSIN+SVS++RCGPGHGISVGSLGRY+EEKDVMGIR
Sbjct: 241 SQSTNINITTSDISVGDDCVSIGPGSINVSVSNVRCGPGHGISVGSLGRYREEKDVMGIR 300

Query: 301 VENCTINGTQNGVRVKTWP-GDQASNATNLTFQNIVMFNVSNPIIIDQQYCPNHSCDTQE 360
           VENCTI  TQNGVRVKTWP GD AS ATNLTFQNIVMFNVSNPIIIDQQYCPNH CD +E
Sbjct: 301 VENCTIKDTQNGVRVKTWPGGDHASKATNLTFQNIVMFNVSNPIIIDQQYCPNHFCDIKE 360

Query: 361 SSSVKLSDINIRNISGTYNSEFAVDLQCSSALPCENIQLSYINLTAIEPPETDDIGRFNC 420
           SS VKLS+INI+NISGTYNSEFAV+LQCSSA+PCEN+QLSYINLTAIEPPETDDIGRFNC
Sbjct: 361 SSGVKLSNINIKNISGTYNSEFAVNLQCSSAMPCENVQLSYINLTAIEPPETDDIGRFNC 420

Query: 421 KGAINNFTILNSTF 433
           KG++NNF+ILNSTF
Sbjct: 421 KGSLNNFSILNSTF 430

BLAST of Tan0012019 vs. NCBI nr
Match: KAG7028995.1 (Exopolygalacturonase-like 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 733.8 bits (1893), Expect = 8.6e-208
Identity = 359/434 (82.72%), Postives = 393/434 (90.55%), Query Frame = 0

Query: 1   MKLSAL-LFSFLLAVSVSARNFSPDPALESAAAATADAWDFLLPPLDMAEPPAGDPWRGL 60
           MKLS+L +F FLLAVSVS RNFSP+PALESAA     +WDFLL P DMAEPP GDPWRGL
Sbjct: 1   MKLSSLVMFYFLLAVSVSGRNFSPEPALESAAG----SWDFLLLPPDMAEPPVGDPWRGL 60

Query: 61  RVFDVKDYGAVADGKTENSLAFMAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFDDL 120
            VFDV DYGA+ADGKTENSLAFM AWEDACAYRGSSTLLIPEG+FF+ +V FSGPCF DL
Sbjct: 61  AVFDVTDYGAIADGKTENSLAFMGAWEDACAYRGSSTLLIPEGSFFLAQVLFSGPCFGDL 120

Query: 121 SPKVLILGTLVAPTTLTADVWIRFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCRQRMT 180
           SPKV+ILGTL+APTTLTADVWI F SLRHLT+TG     ILDGQGA+TW YG +CR R++
Sbjct: 121 SPKVIILGTLIAPTTLTADVWIYFKSLRHLTITGNVGSGILDGQGAETWLYGFNCRHRLS 180

Query: 181 CDIFTTSLKLTDVSNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGIHI 240
           C IFTTSLKL+DVSN  VSNISLVNSKGFH+AL  CDNIHLSGFNITAPW+SQNTDGIHI
Sbjct: 181 CGIFTTSLKLSDVSNATVSNISLVNSKGFHVALNGCDNIHLSGFNITAPWNSQNTDGIHI 240

Query: 241 SQSTNINVTASDIGVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMGIR 300
           SQSTNIN+TASDI VGDDC+SIGPGSIN+SVS++RCGPGHGISVGSLGRY+EEKDV+GIR
Sbjct: 241 SQSTNINITASDISVGDDCVSIGPGSINVSVSNVRCGPGHGISVGSLGRYREEKDVIGIR 300

Query: 301 VENCTINGTQNGVRVKTWP-GDQASNATNLTFQNIVMFNVSNPIIIDQQYCPNHSCDTQE 360
           VENCTI  TQNGVRVKTWP GD AS ATNLTFQNIVMFNVSNPIIIDQQYCPNH CD +E
Sbjct: 301 VENCTIKDTQNGVRVKTWPGGDHASKATNLTFQNIVMFNVSNPIIIDQQYCPNHLCDIKE 360

Query: 361 SSSVKLSDINIRNISGTYNSEFAVDLQCSSALPCENIQLSYINLTAIEPPETDDIGRFNC 420
           SS VKLS+INI+NISGTYNSEFAV+LQCSSA+PCEN+QLSYINLTAIEPPETDDIGRFNC
Sbjct: 361 SSGVKLSNINIKNISGTYNSEFAVNLQCSSAVPCENVQLSYINLTAIEPPETDDIGRFNC 420

Query: 421 KGAINNFTILNSTF 433
           KG++NNF+ILNSTF
Sbjct: 421 KGSLNNFSILNSTF 430

BLAST of Tan0012019 vs. NCBI nr
Match: XP_038885913.1 (exopolygalacturonase-like [Benincasa hispida])

HSP 1 Score: 699.5 bits (1804), Expect = 1.8e-197
Identity = 335/432 (77.55%), Postives = 374/432 (86.57%), Query Frame = 0

Query: 1   MKLSALLFSFLLAVSVSARNFSPDPALESAAAATADAWDFLLPPLDMAEPPAGDPWRGLR 60
           MKLS L+FSFLLA+S+SARNFS            A ++DFLLPP   AE PAGDPW GLR
Sbjct: 1   MKLSLLVFSFLLALSLSARNFS-----------AAGSFDFLLPPSYSAESPAGDPWMGLR 60

Query: 61  VFDVKDYGAVADGKTENSLAFMAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFDDLS 120
           VFDV DYGA+ADGKTENSLAFM+AWEDAC Y GSS +LIPEGNFFV +V+FSGPCF+D+S
Sbjct: 61  VFDVNDYGAIADGKTENSLAFMSAWEDACGYNGSSMVLIPEGNFFVDQVTFSGPCFNDIS 120

Query: 121 PKVLILGTLVAPTTLTADVWIRFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCRQRMTC 180
           PKVLILGTL+APT+L  D WIRF SLR   LTGG   AILDGQGA TW+ GS CR RMTC
Sbjct: 121 PKVLILGTLIAPTSLADDFWIRFQSLRRFILTGGESSAILDGQGAPTWSSGSACRHRMTC 180

Query: 181 DIFTTSLKLTDVSNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGIHIS 240
           D F TSLKL+++SNGIV+NI LV+SKGFH+++Y CDNIHLSGFNITAPWDSQNTDGIHIS
Sbjct: 181 DAFATSLKLSNISNGIVNNIRLVDSKGFHVSIYGCDNIHLSGFNITAPWDSQNTDGIHIS 240

Query: 241 QSTNINVTASDIGVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMGIRV 300
           QSTNI++T SDIGVGDDC+S+GPG +N+S+SDIRCGPGHGISVGSLG+YK E DVMGIRV
Sbjct: 241 QSTNISITISDIGVGDDCVSLGPGCVNVSISDIRCGPGHGISVGSLGKYKNENDVMGIRV 300

Query: 301 ENCTINGTQNGVRVKTWPGDQASNATNLTFQNIVMFNVSNPIIIDQQYCPNHSCDTQESS 360
           ENCTINGTQNGVRVKTWPGD  SNATNLTFQNIVM NVSNPIIIDQ YCPNHSCD QESS
Sbjct: 301 ENCTINGTQNGVRVKTWPGDLVSNATNLTFQNIVMINVSNPIIIDQHYCPNHSCDPQESS 360

Query: 361 SVKLSDINIRNISGTYNSEFAVDLQCSSALPCENIQLSYINLTAIEPPETDDIGRFNCKG 420
           SVKLSDINIRNISGTYN+EFAV++QCS+  PCEN QLSYINLTAI+PP+T  IG FNCKG
Sbjct: 361 SVKLSDINIRNISGTYNTEFAVNIQCSTTKPCENFQLSYINLTAIQPPQTHKIGHFNCKG 420

Query: 421 AINNFTILNSTF 433
           A+N+FTI NSTF
Sbjct: 421 ALNSFTIFNSTF 421

BLAST of Tan0012019 vs. NCBI nr
Match: XP_022941512.1 (exopolygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 618.6 bits (1594), Expect = 4.0e-173
Identity = 295/352 (83.81%), Postives = 324/352 (92.05%), Query Frame = 0

Query: 82  MAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFDDLSPKVLILGTLVAPTTLTADVWI 141
           M AWEDACAYRGSSTLLIPEG+FF+ +V FSGPCF DLSPKV+ILGTL+APTTLTADVWI
Sbjct: 1   MGAWEDACAYRGSSTLLIPEGSFFLAQVLFSGPCFGDLSPKVIILGTLIAPTTLTADVWI 60

Query: 142 RFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCRQRMTCDIFTTSLKLTDVSNGIVSNIS 201
            F SLRHLT+TG     ILDGQGA+TW YG +CR R++C IFTTSLKL+DVSN  VSNIS
Sbjct: 61  YFKSLRHLTITGNVGSGILDGQGAETWLYGFNCRHRLSCGIFTTSLKLSDVSNATVSNIS 120

Query: 202 LVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGIHISQSTNINVTASDIGVGDDCISI 261
           LVNSKGFH+AL  CDNIHLSGFNITAPW+SQNTDGIHISQSTNIN+TASDI VGDDC+SI
Sbjct: 121 LVNSKGFHVALNGCDNIHLSGFNITAPWNSQNTDGIHISQSTNINITASDISVGDDCVSI 180

Query: 262 GPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMGIRVENCTINGTQNGVRVKTWP-GD 321
           GPGSIN+SVS++RCGPGHGISVGSLGRY+EEKDV+GIRVENCTI  TQNGVRVKTWP GD
Sbjct: 181 GPGSINVSVSNVRCGPGHGISVGSLGRYREEKDVIGIRVENCTIKDTQNGVRVKTWPGGD 240

Query: 322 QASNATNLTFQNIVMFNVSNPIIIDQQYCPNHSCDTQESSSVKLSDINIRNISGTYNSEF 381
            AS ATNLTFQNIVMFNVSNPIIIDQQYCPNH CD +ESS VKLS+INI+NISGTYNSEF
Sbjct: 241 HASKATNLTFQNIVMFNVSNPIIIDQQYCPNHLCDIKESSGVKLSNINIKNISGTYNSEF 300

Query: 382 AVDLQCSSALPCENIQLSYINLTAIEPPETDDIGRFNCKGAINNFTILNSTF 433
           AV+LQCSSA+PCEN+QLSYINLTAIEPPETDDIGRFNCKG++NNF+ILNSTF
Sbjct: 301 AVNLQCSSAVPCENVQLSYINLTAIEPPETDDIGRFNCKGSLNNFSILNSTF 352

BLAST of Tan0012019 vs. NCBI nr
Match: XP_004134495.1 (exopolygalacturonase [Cucumis sativus])

HSP 1 Score: 615.1 bits (1585), Expect = 4.5e-172
Identity = 309/442 (69.91%), Postives = 353/442 (79.86%), Query Frame = 0

Query: 1   MKL--SALLFSFLLAVSVSARNFSPDPALESAAAATADAWDFLLPPLDMAEPPAGDPWRG 60
           MKL  S  LFS LLA+++SA                AD +D  LPP   AEPP GDPW G
Sbjct: 1   MKLISSLSLFSSLLAITLSA----------------ADGFDIALPPSYPAEPPVGDPWSG 60

Query: 61  LRVFDVKDYGAVADGKTENSLAFMAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFDD 120
           LRVFDV DYG VADGKT NSLA MAAWEDAC ++G S LLIPEGN+FV +V+FSGPCF+D
Sbjct: 61  LRVFDVNDYGGVADGKTNNSLALMAAWEDACYHQGGSMLLIPEGNYFVDQVTFSGPCFND 120

Query: 121 LSPKVLILGTLVAPTTLTADVWIRFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCRQRM 180
           LSPKVLILGTL+APT LTADVWI FDSLR L+LT GH  + LDG+GA TW+ GS CR  M
Sbjct: 121 LSPKVLILGTLIAPTNLTADVWIHFDSLRRLSLTAGHL-STLDGRGAQTWSSGSRCRHAM 180

Query: 181 TCDIFTTSLKLTDVSNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGIH 240
           TC +F  SLKL++V NG VS+I+LV+SK FH++++ CDNIHLSGFNITAPW SQNTDG H
Sbjct: 181 TCAMFAISLKLSNVLNGTVSSINLVDSKAFHVSIHQCDNIHLSGFNITAPWYSQNTDGFH 240

Query: 241 ISQSTNINVTASDIGVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMGI 300
           ISQSTNI++T S IGVGDDC+SIGPGS+N+ VS++ CGPGHGIS+GSLGRYK E DV GI
Sbjct: 241 ISQSTNISITNSKIGVGDDCVSIGPGSVNVLVSNVTCGPGHGISIGSLGRYKMENDVRGI 300

Query: 301 RVENCTINGTQNGVRVKTWPGDQASNATNLTFQNIVMFNVSNPIIIDQQYCPNHSCDTQE 360
            VENCTI+GTQNGVRVKTWPGD ASNATNLTFQNIVM NVSNPIIIDQQYCPN SCD++E
Sbjct: 301 WVENCTISGTQNGVRVKTWPGDHASNATNLTFQNIVMINVSNPIIIDQQYCPNSSCDSRE 360

Query: 361 SSSVKLSDINIRNISGTYNSEFAVDLQCSSALPCENIQLSYINLTAIEPPETDD------ 420
           SSSVKLSDINIRNISGTYNSEFA++++CSS   CENI+LSYINLT+I     DD      
Sbjct: 361 SSSVKLSDINIRNISGTYNSEFAINMKCSSTKACENIRLSYINLTSINHQSDDDEDEEEG 420

Query: 421 --IGRFNCKGAINNFTILNSTF 433
              G FN KGA+N+FTI NSTF
Sbjct: 421 DVTGNFNFKGALNSFTIFNSTF 425

BLAST of Tan0012019 vs. ExPASy TrEMBL
Match: A0A6J1FMM1 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111446788 PE=3 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 1.9e-173
Identity = 295/352 (83.81%), Postives = 324/352 (92.05%), Query Frame = 0

Query: 82  MAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFDDLSPKVLILGTLVAPTTLTADVWI 141
           M AWEDACAYRGSSTLLIPEG+FF+ +V FSGPCF DLSPKV+ILGTL+APTTLTADVWI
Sbjct: 1   MGAWEDACAYRGSSTLLIPEGSFFLAQVLFSGPCFGDLSPKVIILGTLIAPTTLTADVWI 60

Query: 142 RFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCRQRMTCDIFTTSLKLTDVSNGIVSNIS 201
            F SLRHLT+TG     ILDGQGA+TW YG +CR R++C IFTTSLKL+DVSN  VSNIS
Sbjct: 61  YFKSLRHLTITGNVGSGILDGQGAETWLYGFNCRHRLSCGIFTTSLKLSDVSNATVSNIS 120

Query: 202 LVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGIHISQSTNINVTASDIGVGDDCISI 261
           LVNSKGFH+AL  CDNIHLSGFNITAPW+SQNTDGIHISQSTNIN+TASDI VGDDC+SI
Sbjct: 121 LVNSKGFHVALNGCDNIHLSGFNITAPWNSQNTDGIHISQSTNINITASDISVGDDCVSI 180

Query: 262 GPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMGIRVENCTINGTQNGVRVKTWP-GD 321
           GPGSIN+SVS++RCGPGHGISVGSLGRY+EEKDV+GIRVENCTI  TQNGVRVKTWP GD
Sbjct: 181 GPGSINVSVSNVRCGPGHGISVGSLGRYREEKDVIGIRVENCTIKDTQNGVRVKTWPGGD 240

Query: 322 QASNATNLTFQNIVMFNVSNPIIIDQQYCPNHSCDTQESSSVKLSDINIRNISGTYNSEF 381
            AS ATNLTFQNIVMFNVSNPIIIDQQYCPNH CD +ESS VKLS+INI+NISGTYNSEF
Sbjct: 241 HASKATNLTFQNIVMFNVSNPIIIDQQYCPNHLCDIKESSGVKLSNINIKNISGTYNSEF 300

Query: 382 AVDLQCSSALPCENIQLSYINLTAIEPPETDDIGRFNCKGAINNFTILNSTF 433
           AV+LQCSSA+PCEN+QLSYINLTAIEPPETDDIGRFNCKG++NNF+ILNSTF
Sbjct: 301 AVNLQCSSAVPCENVQLSYINLTAIEPPETDDIGRFNCKGSLNNFSILNSTF 352

BLAST of Tan0012019 vs. ExPASy TrEMBL
Match: A0A0A0L932 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G164560 PE=3 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 2.2e-172
Identity = 309/442 (69.91%), Postives = 353/442 (79.86%), Query Frame = 0

Query: 1   MKL--SALLFSFLLAVSVSARNFSPDPALESAAAATADAWDFLLPPLDMAEPPAGDPWRG 60
           MKL  S  LFS LLA+++SA                AD +D  LPP   AEPP GDPW G
Sbjct: 1   MKLISSLSLFSSLLAITLSA----------------ADGFDIALPPSYPAEPPVGDPWSG 60

Query: 61  LRVFDVKDYGAVADGKTENSLAFMAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFDD 120
           LRVFDV DYG VADGKT NSLA MAAWEDAC ++G S LLIPEGN+FV +V+FSGPCF+D
Sbjct: 61  LRVFDVNDYGGVADGKTNNSLALMAAWEDACYHQGGSMLLIPEGNYFVDQVTFSGPCFND 120

Query: 121 LSPKVLILGTLVAPTTLTADVWIRFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCRQRM 180
           LSPKVLILGTL+APT LTADVWI FDSLR L+LT GH  + LDG+GA TW+ GS CR  M
Sbjct: 121 LSPKVLILGTLIAPTNLTADVWIHFDSLRRLSLTAGHL-STLDGRGAQTWSSGSRCRHAM 180

Query: 181 TCDIFTTSLKLTDVSNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGIH 240
           TC +F  SLKL++V NG VS+I+LV+SK FH++++ CDNIHLSGFNITAPW SQNTDG H
Sbjct: 181 TCAMFAISLKLSNVLNGTVSSINLVDSKAFHVSIHQCDNIHLSGFNITAPWYSQNTDGFH 240

Query: 241 ISQSTNINVTASDIGVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMGI 300
           ISQSTNI++T S IGVGDDC+SIGPGS+N+ VS++ CGPGHGIS+GSLGRYK E DV GI
Sbjct: 241 ISQSTNISITNSKIGVGDDCVSIGPGSVNVLVSNVTCGPGHGISIGSLGRYKMENDVRGI 300

Query: 301 RVENCTINGTQNGVRVKTWPGDQASNATNLTFQNIVMFNVSNPIIIDQQYCPNHSCDTQE 360
            VENCTI+GTQNGVRVKTWPGD ASNATNLTFQNIVM NVSNPIIIDQQYCPN SCD++E
Sbjct: 301 WVENCTISGTQNGVRVKTWPGDHASNATNLTFQNIVMINVSNPIIIDQQYCPNSSCDSRE 360

Query: 361 SSSVKLSDINIRNISGTYNSEFAVDLQCSSALPCENIQLSYINLTAIEPPETDD------ 420
           SSSVKLSDINIRNISGTYNSEFA++++CSS   CENI+LSYINLT+I     DD      
Sbjct: 361 SSSVKLSDINIRNISGTYNSEFAINMKCSSTKACENIRLSYINLTSINHQSDDDEDEEEG 420

Query: 421 --IGRFNCKGAINNFTILNSTF 433
              G FN KGA+N+FTI NSTF
Sbjct: 421 DVTGNFNFKGALNSFTIFNSTF 425

BLAST of Tan0012019 vs. ExPASy TrEMBL
Match: A0A6J1I855 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470865 PE=3 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 5.3e-171
Identity = 292/352 (82.95%), Postives = 320/352 (90.91%), Query Frame = 0

Query: 82  MAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFDDLSPKVLILGTLVAPTTLTADVWI 141
           M AWEDACAYRGSST LIPEG+FF+ +V FSGPCF DLSPKV+ILGTL+APTTLTADVWI
Sbjct: 1   MGAWEDACAYRGSSTFLIPEGSFFLAQVLFSGPCFGDLSPKVIILGTLIAPTTLTADVWI 60

Query: 142 RFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCRQRMTCDIFTTSLKLTDVSNGIVSNIS 201
            F SLR LT+TG     ILDGQGA+TW YG +CR R++C IFTTSLKL+DVSN  VSNIS
Sbjct: 61  YFKSLRQLTITGNIGSGILDGQGAETWLYGFNCRHRLSCGIFTTSLKLSDVSNATVSNIS 120

Query: 202 LVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGIHISQSTNINVTASDIGVGDDCISI 261
           LVNSKGFH+AL  CDNIHLSGF ITAPW+SQNTDGIHISQSTNIN+TASDI VGDDC+SI
Sbjct: 121 LVNSKGFHVALNGCDNIHLSGFKITAPWNSQNTDGIHISQSTNINITASDISVGDDCVSI 180

Query: 262 GPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMGIRVENCTINGTQNGVRVKTWPG-D 321
           GPGSIN+SVS++ CGPGHGISVGSLGRY+EEKDVMGIRVENCTI  TQNGVRVKTWPG D
Sbjct: 181 GPGSINVSVSNVICGPGHGISVGSLGRYREEKDVMGIRVENCTIKDTQNGVRVKTWPGED 240

Query: 322 QASNATNLTFQNIVMFNVSNPIIIDQQYCPNHSCDTQESSSVKLSDINIRNISGTYNSEF 381
            AS ATNLTFQNIVMFNVSNPIIIDQQYCPNH CD +ESS VKLS+INI+NISGTYNSEF
Sbjct: 241 HASKATNLTFQNIVMFNVSNPIIIDQQYCPNHFCDIKESSGVKLSNINIKNISGTYNSEF 300

Query: 382 AVDLQCSSALPCENIQLSYINLTAIEPPETDDIGRFNCKGAINNFTILNSTF 433
           AV+LQCSSA+PCEN+QLSYINLTAIEPPETDDIGRFNCKG++NNF+ILNSTF
Sbjct: 301 AVNLQCSSAMPCENVQLSYINLTAIEPPETDDIGRFNCKGSLNNFSILNSTF 352

BLAST of Tan0012019 vs. ExPASy TrEMBL
Match: A0A6J1GXB0 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111457998 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 1.3e-153
Identity = 260/351 (74.07%), Postives = 303/351 (86.32%), Query Frame = 0

Query: 82  MAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFDDLSPKVLILGTLVAPTTLTADVWI 141
           MAAWE+ACA++G S  LIPEG F V  V+FSGPCF++LSPKVLI  TL+APT LT DVWI
Sbjct: 1   MAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDVWI 60

Query: 142 RFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCRQRMTCDIFTTSLKLTDVSNGIVSNIS 201
            F SLRHL LTG    A+L+GQGA TW++GS CR+RMTC IFTTSLKLTDVS+ IV +IS
Sbjct: 61  HFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHAIVKDIS 120

Query: 202 LVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGIHISQSTNINVTASDIGVGDDCISI 261
           LV+SKGFH+A++ CD+IHL GFNITAPW+SQNTDGIHIS STNINV  S IGVGDDC+SI
Sbjct: 121 LVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDCVSI 180

Query: 262 GPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMGIRVENCTINGTQNGVRVKTWPGDQ 321
           GPGS ++ VS+IRCGPGHGISVGSLGRYK E DV+GIRVENCTI+ TQNGVRVKTWPG+ 
Sbjct: 181 GPGSFDVLVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWPGNY 240

Query: 322 ASNATNLTFQNIVMFNVSNPIIIDQQYCPNHSCDTQESSSVKLSDINIRNISGTYNSEFA 381
           +SNA +LTFQNIVM NVSNPIIIDQ+YCPNHSCD +ESSSVKLS++N+RNISGTYN++FA
Sbjct: 241 SSNAAHLTFQNIVMNNVSNPIIIDQRYCPNHSCDMKESSSVKLSEMNMRNISGTYNTKFA 300

Query: 382 VDLQCSSALPCENIQLSYINLTAIEPPETDDIGRFNCKGAINNFTILNSTF 433
           V++ CSS +PC N+ LSYINLTA  PPE  +IGRFNC GA+N FT+LNSTF
Sbjct: 301 VNIDCSSIMPCNNVHLSYINLTATHPPEKYEIGRFNCMGALNTFTVLNSTF 351

BLAST of Tan0012019 vs. ExPASy TrEMBL
Match: A0A6J1C8D5 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111009318 PE=3 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 5.2e-150
Identity = 264/355 (74.37%), Postives = 301/355 (84.79%), Query Frame = 0

Query: 82  MAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFDDLSPKVLILGTLVAPTTL-TADVW 141
           M AWE+ACAY G S L +P+G FFVGE  FSGPCF+DLSPKVLILGTL APT L  ADVW
Sbjct: 1   MVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKVLILGTLRAPTALDDADVW 60

Query: 142 IRFDSLRHLTLTGG--HQPAILDGQGADTWTYGSDCRQRMTCDIFTTSLKLTDVSNGIVS 201
           I F SL HL +TGG  +  AILDGQGA TW+  S CR+  TCDIF TSLKL+DVS G VS
Sbjct: 61  IHFKSLHHLDITGGDTNTTAILDGQGAQTWS-NSACRRHFTCDIFATSLKLSDVSYGRVS 120

Query: 202 NISLVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGIHISQSTNINVTASDIGVGDDC 261
           NI +V+SKGFH+AL+ CDNIHLSGFNI AP DSQNTDGIH+SQSTNIN+T+SDIGVGDDC
Sbjct: 121 NIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIHVSQSTNINITSSDIGVGDDC 180

Query: 262 ISIGPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMGIRVENCTINGTQNGVRVKTWP 321
           +SIGPGS+N+SVSDI CGPGHGISVGSLGRY+ E DV+GIRVENC+INGT NGVR+KTWP
Sbjct: 181 VSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGIRVENCSINGTTNGVRIKTWP 240

Query: 322 GDQASNATNLTFQNIVMFNVSNPIIIDQQYCPNHSCDTQESSSVKLSDINIRNISGTYNS 381
           G  +S ATNLTFQNIVM NVSNPI+IDQQYCPNHSC T E+S+VKLS+I +RNISG+YNS
Sbjct: 241 GQHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHEASNVKLSEIKMRNISGSYNS 300

Query: 382 EFAVDLQCSSALPCENIQLSYINLTAIEPPETD-DIGRFNCKGAINNFTILNSTF 433
           EFAV+LQCSS   CE I+LSYINLTA +PP+ +  IGRFNC GA++ FTILNSTF
Sbjct: 301 EFAVNLQCSSGKACEKIELSYINLTATQPPDQNHQIGRFNCNGALHAFTILNSTF 354

BLAST of Tan0012019 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 308.9 bits (790), Expect = 6.4e-84
Identity = 161/342 (47.08%), Postives = 219/342 (64.04%), Query Frame = 0

Query: 63  DVKDYGAVADGKTENSLAFMAAWEDACAYRGSS-TLLIPEGNFFVGEVSFSGPCFDDLSP 122
           DV+ +GA A+   +++ AF+AAW+ AC    SS  L+IP G F VG + FSGPC +  + 
Sbjct: 36  DVRSFGARANDHRDHTKAFVAAWDKACKSSSSSVNLIIPRGEFSVGSLRFSGPCTNVSNL 95

Query: 123 KVLILGTLVAPTTLTADVWIRFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCRQRMTCD 182
            V +  +       +   WI+F  +  LTLTGG      DGQGA  W + ++C     C 
Sbjct: 96  TVRVKASTDLSKYRSGGGWIQFGWINGLTLTGG---GTFDGQGALAWPF-NNCTSDSNCK 155

Query: 183 IFTTSLKLTDVSNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTDGIHISQ 242
           +  TSLK   ++  +V  IS VNSK FH+AL +C +   +  NITAP DS NTDGIHI +
Sbjct: 156 LLPTSLKFVGMNRTVVRRISSVNSKFFHIALVECRDFKGTRLNITAPSDSPNTDGIHIER 215

Query: 243 STNINVTASDIGVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYKEEKDVMGIRVE 302
           S+N+  + S I  GDDC+SIG G+  I+++ I+CGPGHGISVGSLGRY  EKDV G+ V+
Sbjct: 216 SSNVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNEKDVNGLVVK 275

Query: 303 NCTINGTQNGVRVKTWPGDQA-SNATNLTFQNIVMFNVSNPIIIDQQYCPNHSCDTQESS 362
           +C I+GT NG+R+KTW      S ATN+TF+NI+M NV+NPIIIDQ YCP  SC +   S
Sbjct: 276 DCKISGTTNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSYCPFSSCISNVPS 335

Query: 363 SVKLSDINIRNISGTYNSEFAVDLQCSSALPCENIQLSYINL 403
            V+LS+I  +NI GT +S  AV L CS  +PC+ + L  ++L
Sbjct: 336 KVELSEIYFKNIRGTSSSLVAVQLHCSRGMPCKKVYLENVHL 373

BLAST of Tan0012019 vs. TAIR 10
Match: AT2G33160.1 (glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein )

HSP 1 Score: 303.5 bits (776), Expect = 2.7e-82
Identity = 153/350 (43.71%), Postives = 228/350 (65.14%), Query Frame = 0

Query: 60  RVFDVKDYGAVADGKTENSLAFMAAWEDACAYR-GSSTLLIPEGNFFVGEVSFSGPCFDD 119
           ++FDV++YGA AD + +N+ AF  AW +AC +  G ST+ IP G F++ +V+FSGPC   
Sbjct: 30  KIFDVRNYGARADSQRDNAFAFTKAWNEACQWSYGRSTVYIPSGIFYLRQVTFSGPCKSS 89

Query: 120 LSPKVLILGTLVAPT---TLTADVWIRFDSLRHLTLTGGHQPAILDGQGADTWTYGSDCR 179
           ++    I GTL+AP     +  + WI F  + +LT+TGG    +LDGQG+ +W   +DC 
Sbjct: 90  IT--FFIRGTLLAPRNPYAINQEEWILFKYVDNLTVTGG---GLLDGQGSYSWPL-NDCN 149

Query: 180 QRMTCDIFTTSLKLTDVSNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWDSQNTD 239
           +   C     ++    V +  ++ +  +NSK  H  L+  ++ +++G  ITAP DS NTD
Sbjct: 150 KNTNCRTLAMNIGFAFVKSSKINGLRSINSKMGHFNLFSVEDFNITGVTITAPGDSPNTD 209

Query: 240 GIHISQSTNINVTASDIGVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYKEEKDV 299
           GI I +S+++ +    IG GDDCI+I  G+ N+ +SD+RCGPGHGISVGSLGRYKEEK+V
Sbjct: 210 GIKIGKSSHMQIYNVTIGTGDDCIAILDGTSNLDISDVRCGPGHGISVGSLGRYKEEKNV 269

Query: 300 MGIRVENCTINGTQNGVRVKTWPGDQAS-NATNLTFQNIVMFNVSNPIIIDQQYCPNHSC 359
            G+ V N  INGT +G+R+KTW    +  + +N  ++NI M NV NPI+IDQQYCP+  C
Sbjct: 270 QGLTVRNSIINGTTDGLRIKTWAKSVSQISVSNFLYENIQMINVGNPIVIDQQYCPHGQC 329

Query: 360 DT--QESSSVKLSDINIRNISGTYNSEFAVDLQCSSALPCENIQLSYINL 403
           D+  + +S V++ D+   NI GT  S+ A+ +QCS   PC++++LS INL
Sbjct: 330 DSPGKYASHVQIKDVKYNNIWGTSTSKEALKMQCSKTFPCQDVELSNINL 373

BLAST of Tan0012019 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 302.4 bits (773), Expect = 6.0e-82
Identity = 174/412 (42.23%), Postives = 238/412 (57.77%), Query Frame = 0

Query: 2   KLSALLFSFLLAVSVSARNFSP--DPALESAAAATADAWDFLLPPLDMAEPPAGDPWRGL 61
           K S +L   L+ ++ +  N +P  D A ++AA A  D        +  A    G    G 
Sbjct: 6   KASGVLLVLLVVMATTIANGTPVVDTA-KNAATAVEDTAKNAATAVGGAAASVGAKVTGA 65

Query: 62  R----VFDVKDYGAVADGKTENSLAFMAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPC 121
           +      DVK  GA  DGKT++S AF AAW++ACA    ST+ +P+G + V  + F GPC
Sbjct: 66  KPGGASLDVKASGAKGDGKTDDSAAFAAAWKEACA--AGSTITVPKGEYLVESLEFKGPC 125

Query: 122 FDDLSPKVLIL-GTLVAPTTLTADV----WIRFDSLRHLTLTGGHQPAILDGQGADTWTY 181
                P  L L G   AP T+        WI F++L   TL G    AI DGQG+  W  
Sbjct: 126 ---KGPVTLELNGNFKAPATVKTTKPHAGWIDFENLADFTLNG--NKAIFDGQGSLAWK- 185

Query: 182 GSDCRQRMTCDIFTTSLKLTDVSNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWD 241
            +DC +   C+    +++ T ++N  +++I+  NSK FHM + +C NI L+   I AP +
Sbjct: 186 ANDCAKTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPE 245

Query: 242 SQNTDGIHISQSTNINVTASDIGVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYK 301
           S NTDGIHI +S  +N+  + I  GDDC+SIG G+ N+ V ++ CGPGHGIS+GSLGRY 
Sbjct: 246 SLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYP 305

Query: 302 EEKDVMGIRVENCTINGTQNGVRVKTWPGDQASNATNLTFQNIVMFNVSNPIIIDQQYCP 361
            E+ V G+ V  C I  T NGVR+KTWPG     A+N+ F++I M NVS P++IDQ+YCP
Sbjct: 306 NEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCP 365

Query: 362 NHSCDTQESSSVKLSDINIRNISGTYNSEFAVDLQCSSALPCENIQLSYINL 403
              C     S VKLSD+ I+NI GT  ++ AV L CS  +PC NI LS INL
Sbjct: 366 YGHCKAGVPSKVKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINL 408

BLAST of Tan0012019 vs. TAIR 10
Match: AT1G78400.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 300.4 bits (768), Expect = 2.3e-81
Identity = 153/349 (43.84%), Postives = 218/349 (62.46%), Query Frame = 0

Query: 58  GLRVFDVKDYGAVADGKTENSLAFMAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCFD 117
           G +VFDV+ YGA  DGKT+N++AF  AW+DAC ++G   + IP G F++G V+F+GPC  
Sbjct: 31  GRKVFDVRSYGARGDGKTDNTMAFTKAWKDACQWKGLPRVYIPFGTFYLGAVAFTGPCKS 90

Query: 118 DLSPKVLILGTLVA---PTTLTADVWIRFDSLRHLTLTGGHQPAILDGQGADTWTYGSDC 177
            +S   +I GTL+A   P  +  D WI F  + +LT++GG    ILDGQG+ +W   ++C
Sbjct: 91  RIS--FIIKGTLLAPKDPNAIKQDSWIIFRYVDYLTVSGG---GILDGQGSYSWPL-NNC 150

Query: 178 RQRMTCDIFTTSLKLTDVSNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWDSQNT 237
           RQ   C     ++    V    ++ I  +NSK  H+  +   +  ++  NI AP DS NT
Sbjct: 151 RQTHNCRALPMNMGFQFVRFSRLTRIKSINSKMGHLNFFSVQHFDITRVNIKAPGDSPNT 210

Query: 238 DGIHISQSTNINVTASDIGVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYKEEKD 297
           DGI I  S ++ +   DI  GDDCI+I  G+ N+ ++ + CGPGHGISVGSLG++K EK 
Sbjct: 211 DGIKIGSSNHMKIHHVDIATGDDCIAILSGTFNLDINKVNCGPGHGISVGSLGKFKGEKS 270

Query: 298 VMGIRVENCTINGTQNGVRVKTWPGDQASN-ATNLTFQNIVMFNVSNPIIIDQQYCPNHS 357
           V G+ V N   NGT NGVR+KTWP     N  +N  F+N+ M +V +PI IDQ+YCPN  
Sbjct: 271 VQGLIVRNSIFNGTSNGVRIKTWPSPGEPNLVSNFLFKNLQMIDVQSPINIDQRYCPNPP 330

Query: 358 CDTQESSSVKLSDINIRNISGTYNSEFAVDLQCSSALPCENIQLSYINL 403
           C  Q  S +++ D+  +NI GT  ++ AV LQCS  +PC+N+QL  IN+
Sbjct: 331 CSFQSFSKIQIRDVKFQNIWGTSTAKEAVKLQCSKNVPCKNVQLFNINI 373

BLAST of Tan0012019 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 298.9 bits (764), Expect = 6.6e-81
Identity = 172/411 (41.85%), Postives = 236/411 (57.42%), Query Frame = 0

Query: 2   KLSALLFSFLLAVSVSARNFSP--DPALESAAAATADAWDFLLPPLDMAEPPAGDPWRGL 61
           K S +L   L+ ++ +  N +P  D A ++AA A  D        +  A    G    G 
Sbjct: 6   KASGVLLVLLVVMATTIANGTPVVDKA-KNAATAVEDTAKNAATAVGGAAASVGAKVSGA 65

Query: 62  R---VFDVKDYGAVADGKTENSLAFMAAWEDACAYRGSSTLLIPEGNFFVGEVSFSGPCF 121
           +     DVK  GA  D KT++S AF AAW++ACA    ST+ +P+G + V  + F GPC 
Sbjct: 66  KPGAAVDVKASGAKGDSKTDDSAAFAAAWKEACA--AGSTITVPKGEYMVESLEFKGPC- 125

Query: 122 DDLSPKVLIL-GTLVAPTTLTADV----WIRFDSLRHLTLTGGHQPAILDGQGADTWTYG 181
               P  L L G   AP T+        WI F+++   TL G    AI DGQG+  W   
Sbjct: 126 --KGPVTLELNGNFKAPATVKTTKPHAGWIDFENIADFTLNG--NKAIFDGQGSLAWK-A 185

Query: 182 SDCRQRMTCDIFTTSLKLTDVSNGIVSNISLVNSKGFHMALYDCDNIHLSGFNITAPWDS 241
           +DC +   C+    +++ T ++N  +++I+  NSK FHM + +C NI LS   I AP +S
Sbjct: 186 NDCAKTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPES 245

Query: 242 QNTDGIHISQSTNINVTASDIGVGDDCISIGPGSINISVSDIRCGPGHGISVGSLGRYKE 301
            NTDGIHI +S  +N+  + I  GDDC+SIG G+ N+ V ++ CGPGHGIS+GSLGRY  
Sbjct: 246 LNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPN 305

Query: 302 EKDVMGIRVENCTINGTQNGVRVKTWPGDQASNATNLTFQNIVMFNVSNPIIIDQQYCPN 361
           E+ V G+ V  C I  T NGVR+KTWPG     A+N+ F++I M NVS P++IDQ+YCP 
Sbjct: 306 EQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPY 365

Query: 362 HSCDTQESSSVKLSDINIRNISGTYNSEFAVDLQCSSALPCENIQLSYINL 403
             C     S VKLSD+ I+ I GT  ++ AV L CS  +PC NI LS INL
Sbjct: 366 GHCKAGVPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINL 407