Tan0012012 (gene) Snake gourd v1

Overview


Name	Tan0012012
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	AB hydrolase-1 domain-containing protein
Location	LG02: 9699877 .. 9702206 (+)
RNA-Seq Expression	Tan0012012
Synteny	Tan0012012

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAGAGAGATTAAACAACATATATCAAGAATGCAAAAATTTTTGCTACCATTTTTGTTTCTTATTTTTCTCTCTTTCTCCTCCCTAACACGTAGTTCAATTCTCCTCCATGATCACTCTCAATCGAGCACTTCGCACTTTGTACTCGTCCATGGCGCTTGTCTCGGTGCCTGGTCTTGGTACAAGGTCACCACTTTGTTGCAAACAGCCGGTCACAATGTTACCGCACTCGACATGGCTGCTGCCGGGATCAACCAGACGCAACCTGAGTCGTTGACATCGCTTACCGAGTACTACGAGCCACTCTTGAGCTTCATGGAAGCCCTGCCTTCCGATGAGAAGATCGTTCTCGTCGGCCACAGCCTCGGCGGCCTCGGCATCTCGATGGCCATGGAGAGATTCCCTGAGAAAATCTCCGTTGCCATTTTTGTCACTGCAGCCATGCCTGGTCCCATTGGAGGGATTTTTACCATAGATGAACTGGTTTAAAATCTAACTCTAATTCCTTCAAATTTTCACTCCATATCTTGTTCTATACAAGTAATGAAGTTTTTTTTTTGACAACAAAATTAGAAGGGAATACTTGAAATGTTCAGAGCCTTCTACTCAGAGGAATCCAAAGCTTCTGATTCAAACCTATTCCTATTCCCGAAAGAAGAATTAGAAACCAAGCTTTTTCCACTCTCGCCACCTGAGGTTCGTACTTCTGATCTCTTTTAACTTAAAGTTCAGGTCGGGCAGAGAAGGAAGGAGAGAGTGATTGTCCCTCTTAATTTGGGGTTTTTTTTAAAAAAAAATTAAATATATATCAAATATAGTCTTATTTTATTAAGTTTCAATTTGGTCTTTCAATTTTTTAAGATCCTCAATTTTTAACAAAGTTTTCACATGAAAAAAAGAGTAAAAATGTGTGGTTAAGTTATTGTTTTATCTAACATATTTGGGCACAATATGCAACTTGAACTTGTCATATCTAGAAGAAATAGTTTGTGTTGATGGACGGGTTAAATTTTGTTTATGGATGAAAACATTTGGGTGGCTTGTCCTTCAAATACATTAGTAAGACAATAACTTTCATTATACTAAAACGAAAAAAAAGCTTAAAAGCTAAGGATGAAATTGGAAATTTTATATATATATATATATATATTAACAACTAATAAGTTAAGATTTCAACTTTTGTCGTTTTAGGCATTTTTAAATTTATTCTCTATTGTTTAAAAATTTTCAATTTAGTCTCTTCATGTATTAACATTTTTCAATTTAGTCTCTCATAGTAGGTAAATGTTAAAATTAGTTTATGACAAATTAACATGACATAATACTTAGTGAATTGAAGGAATTAAAAATTCAAATTTTACAAAAAATTCTTAGAAATGTGGAGGATGAACCAAAAACTTCCCTATCTCAAGTCTCATTTGATAATCATATGATTTTTTTTTTCTATTTTTAGTTTATGAAAATTAAACCTATAAATACTATTTTCACCCATGAGTTTCTTTATTTTGTTGTCTCCTTATGTTTTCAAAAACCAAATCCAAGTTTTGAAAACTAAAAAAAAAAAGTAGTTTTTAAAAACTTGTTTTTATTTTTGGAATTTATTTAATGAAGAATTCTAATGTTTTTTTTTTTGTTTTTGAAGAAAGATGAAACTCATTATAGAGAAATTAAAGATGGAGAAAACAAAGATAATTTTAAAAAACAAAATGATATTATCGTCAAATGGGACCTTAGATAACCATTGTCGTCCACTACTAAATCTCAAATTCACTAGGTATTATAAGTTTATCACTAGTCAATCTCAACGCTCTTTAAAAAGGGAAAAACTAAAGCGAACTATAGATCATCAGCTACCTCTAGTAATAACTAATAGTATTGGAATGTTGGCTGGCATATATATATATATATATAGGATCTAACATTGGCAACAACACTGGTGAGGCCGCAAGCAATGTTCGGGATGTTGGAGTCAATGAAGGAACTAGCACTGAGCAGAGACAAATATGGGTCAGTGAAAAGGGCTTTCATTATATCAGAGAAGGACTTGATGACGCCCAAGATATTAGTGTGGGCAATGCTCACATTCAACAACCCCGATCTTCAGGCTCAGATTCAAGGCTCAGATCACATGGTCATGGCTTCCAAGCCCCTTCAACTTGCCCAACAACTCGCCACCATCGCTCAACACTTTGCTTCTTCTTCTTCTTCTTATAATTAATGATGATGATGATATGTAATTATTATATATATATATGTGTATATGTGTGTATTATGGTCTCTCTTTTCTTCCTCTTCACATTCACAATAATAAAGTGTTTGGGTTTGAATTGA

mRNA sequence

GCAGAGAGATTAAACAACATATATCAAGAATGCAAAAATTTTTGCTACCATTTTTGTTTCTTATTTTTCTCTCTTTCTCCTCCCTAACACGTAGTTCAATTCTCCTCCATGATCACTCTCAATCGAGCACTTCGCACTTTGTACTCGTCCATGGCGCTTGTCTCGGTGCCTGGTCTTGGTACAAGGTCACCACTTTGTTGCAAACAGCCGGTCACAATGTTACCGCACTCGACATGGCTGCTGCCGGGATCAACCAGACGCAACCTGAGTCGTTGACATCGCTTACCGAGTACTACGAGCCACTCTTGAGCTTCATGGAAGCCCTGCCTTCCGATGAGAAGATCGTTCTCGTCGGCCACAGCCTCGGCGGCCTCGGCATCTCGATGGCCATGGAGAGATTCCCTGAGAAAATCTCCGTTGCCATTTTTGTCACTGCAGCCATGCCTGGTCCCATTGGAGGGATTTTTACCATAGATGAACTGAAGGGAATACTTGAAATGTTCAGAGCCTTCTACTCAGAGGAATCCAAAGCTTCTGATTCAAACCTATTCCTATTCCCGAAAGAAGAATTAGAAACCAAGCTTTTTCCACTCTCGCCACCTGAGGATCTAACATTGGCAACAACACTGGTGAGGCCGCAAGCAATGTTCGGGATGTTGGAGTCAATGAAGGAACTAGCACTGAGCAGAGACAAATATGGGTCAGTGAAAAGGGCTTTCATTATATCAGAGAAGGACTTGATGACGCCCAAGATATTAGTGTGGGCAATGCTCACATTCAACAACCCCGATCTTCAGGCTCAGATTCAAGGCTCAGATCACATGGTCATGGCTTCCAAGCCCCTTCAACTTGCCCAACAACTCGCCACCATCGCTCAACACTTTGCTTCTTCTTCTTCTTCTTATAATTAATGATGATGATGATATGTAATTATTATATATATATATGTGTATATGTGTGTATTATGGTCTCTCTTTTCTTCCTCTTCACATTCACAATAATAAAGTGTTTGGGTTTGAATTGA

Coding sequence (CDS)

ATGCAAAAATTTTTGCTACCATTTTTGTTTCTTATTTTTCTCTCTTTCTCCTCCCTAACACGTAGTTCAATTCTCCTCCATGATCACTCTCAATCGAGCACTTCGCACTTTGTACTCGTCCATGGCGCTTGTCTCGGTGCCTGGTCTTGGTACAAGGTCACCACTTTGTTGCAAACAGCCGGTCACAATGTTACCGCACTCGACATGGCTGCTGCCGGGATCAACCAGACGCAACCTGAGTCGTTGACATCGCTTACCGAGTACTACGAGCCACTCTTGAGCTTCATGGAAGCCCTGCCTTCCGATGAGAAGATCGTTCTCGTCGGCCACAGCCTCGGCGGCCTCGGCATCTCGATGGCCATGGAGAGATTCCCTGAGAAAATCTCCGTTGCCATTTTTGTCACTGCAGCCATGCCTGGTCCCATTGGAGGGATTTTTACCATAGATGAACTGAAGGGAATACTTGAAATGTTCAGAGCCTTCTACTCAGAGGAATCCAAAGCTTCTGATTCAAACCTATTCCTATTCCCGAAAGAAGAATTAGAAACCAAGCTTTTTCCACTCTCGCCACCTGAGGATCTAACATTGGCAACAACACTGGTGAGGCCGCAAGCAATGTTCGGGATGTTGGAGTCAATGAAGGAACTAGCACTGAGCAGAGACAAATATGGGTCAGTGAAAAGGGCTTTCATTATATCAGAGAAGGACTTGATGACGCCCAAGATATTAGTGTGGGCAATGCTCACATTCAACAACCCCGATCTTCAGGCTCAGATTCAAGGCTCAGATCACATGGTCATGGCTTCCAAGCCCCTTCAACTTGCCCAACAACTCGCCACCATCGCTCAACACTTTGCTTCTTCTTCTTCTTCTTATAATTAA

Protein sequence

MQKFLLPFLFLIFLSFSSLTRSSILLHDHSQSSTSHFVLVHGACLGAWSWYKVTTLLQTAGHNVTALDMAAAGINQTQPESLTSLTEYYEPLLSFMEALPSDEKIVLVGHSLGGLGISMAMERFPEKISVAIFVTAAMPGPIGGIFTIDELKGILEMFRAFYSEESKASDSNLFLFPKEELETKLFPLSPPEDLTLATTLVRPQAMFGMLESMKELALSRDKYGSVKRAFIISEKDLMTPKILVWAMLTFNNPDLQAQIQGSDHMVMASKPLQLAQQLATIAQHFASSSSSYN

Homology

[Close]

BLAST of Tan0012012 vs. ExPASy Swiss-Prot
Match: O80477 (Methylesterase 3 OS=Arabidopsis thaliana OX=3702 GN=MES3 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.3e-45
Identity = 109/256 (42.58%), Postives = 153/256 (59.77%), Query Frame = 0


Query: 36  HFVLVHGACLGAWSWYKVTTLLQTAGHNVTALDMAAAGINQTQP-ESLTSLTEYYEPLLS 95
           H VLVHGAC GAW WYKV   L+ +GH VTA+D+AA+GI+ T+    +++  +Y EPL+ 
Sbjct: 9   HVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYSEPLMQ 68

Query: 96  FMEALPSDEKIVLVGHSLGGLGISMAMERFPEKISVAIFVTAAMPG-PIGGIFTIDELK- 155
            M +LP DEK+VLVGHSLGGL ++MAM+ FP KISV++FVTA MP       F  D+L+ 
Sbjct: 69  LMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSFVWDKLRK 128

Query: 156 --GILEMFRAFYSEESKASDSNLFLFPKEELETKLFPLSPPEDLTLATTLVRPQAMFGML 215
                E     ++ E     S  ++F  E +   L+ LSP +DL LA  LVR   +    
Sbjct: 129 ETSREEWLDTVFTSEKPDFPSEFWIFGPEFMAKNLYQLSPVQDLELAKMLVRANPLI-KK 188

Query: 216 ESMKELALSRDKYGSVKRAFIISEKDLMTPKILVWAMLTFNNPDLQAQIQGSDHMVMASK 275
           +  +  + S + YGSV R FI+  KDL++P+    +M++   P    +I+ +DHM M SK
Sbjct: 189 DMAERRSFSEEGYGSVTRIFIVCGKDLVSPEDYQRSMISNFPPKEVMEIKDADHMPMFSK 248

Query: 276 PLQLAQQLATIAQHFA 287
           P QL   L  IA  +A
Sbjct: 249 PQQLCALLLEIANKYA 263

[Close]

BLAST of Tan0012012 vs. ExPASy Swiss-Prot
Match: O80475 (Methylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=MES8 PE=3 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 7.3e-44
Identity = 105/254 (41.34%), Postives = 151/254 (59.45%), Query Frame = 0


Query: 36  HFVLVHGACLGAWSWYKVTTLLQTAGHNVTALDMAAAGINQTQPESLTSLTEYYEPLLSF 95
           HFVLVHG+CLGAW WYKV  LL+ +GH VTALD+AA GI+      +++  +Y EPL+  
Sbjct: 27  HFVLVHGSCLGAWCWYKVKPLLEASGHRVTALDLAACGIDTRSITDISTCEQYSEPLIQL 86

Query: 96  MEALPSDEKIVLVGHSLGGLGISMAMERFPEKISVAIFVTAAMPGPIGGIFTIDELKGIL 155
           M +LP+DEK+VLVGHS GGL +++AM++FP+KISV++FVT+ MP       T +    +L
Sbjct: 87  MTSLPNDEKVVLVGHSYGGLTLAIAMDKFPDKISVSVFVTSFMPD------TKNSPSFVL 146

Query: 156 EMFRAFYSEESKASD--SNLFLFPKEELETKLFPLSPPEDLTLATTLVRPQAMF-GMLES 215
           E F +  + E           +F  E  + ++  LSP EDL L   L RP ++F   L  
Sbjct: 147 EKFASTMTPEDWMGSELEPYVVFSAEFTKHRILQLSPIEDLELRLLLKRPGSLFLNDLSR 206

Query: 216 MKELALSRDKYGSVKRAFIISEKDLMTPKILVWAMLTFNNPDLQAQIQGSDHMVMASKPL 275
           MK    S   YGSV RA+I+S+ D    +     M+    P+L  +++G+DH+ +  KP 
Sbjct: 207 MKN--FSEKGYGSVPRAYIVSKDDHTISEEYQRWMIDNYPPNLVIEMEGTDHLPLFCKPQ 266

Query: 276 QLAQQLATIAQHFA 287
            L+  L  IA  F+
Sbjct: 267 LLSDHLLAIADKFS 272

[Close]

BLAST of Tan0012012 vs. ExPASy Swiss-Prot
Match: Q6RYA0 (Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.0e-42
Identity = 102/258 (39.53%), Postives = 153/258 (59.30%), Query Frame = 0


Query: 36  HFVLVHGACLGAWSWYKVTTLLQTAGHNVTALDMAAAGINQTQPESLTSLTEYYEPLLSF 95
           HFVLVHGAC G WSWYK+  LL+ AGH VTALD+AA+G +  + E L +L +Y  PL+  
Sbjct: 6   HFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASGTDLRKIEELRTLYDYTLPLMEL 65

Query: 96  MEALPSDEKIVLVGHSLGGLGISMAMERFPEKISVAIFVTAAMPGPI-GGIFTIDEL--- 155
           ME+L +DEK++LVGHSLGG+ + +AME++P+KI  A+F+ A MP  +    F +++    
Sbjct: 66  MESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFMPDSVHNSSFVLEQYNER 125

Query: 156 ----KGILEMFRAFYSEESKASDSNLFLFPKEELETKLFPLSPPEDLTLATTLVRPQAMF 215
                 +   F  + S E     +++F  PK  L  KL+ L  PEDL LA++LVRP ++F
Sbjct: 126 TPAENWLDTQFLPYGSPEEPL--TSMFFGPK-FLAHKLYQLCSPEDLALASSLVRPSSLF 185

Query: 216 GMLESMKELALSRDKYGSVKRAFIISEKDLMTPKILVWAMLTFNNPDLQAQIQGSDHMVM 275
            M +  K    + +++GSVKR +I+  +D   P+      +         +I+G+DHM M
Sbjct: 186 -MEDLSKAKYFTDERFGSVKRVYIVCTEDKGIPEEFQRWQIDNIGVTEAIEIKGADHMAM 245

Query: 276 ASKPLQLAQQLATIAQHF 286
             +P +L   L  IA  +
Sbjct: 246 LCEPQKLCASLLEIAHKY 259

[Close]

BLAST of Tan0012012 vs. ExPASy Swiss-Prot
Match: Q8S8S9 (Methylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=MES1 PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.8e-42
Identity = 107/256 (41.80%), Postives = 152/256 (59.38%), Query Frame = 0


Query: 36  HFVLVHGACLGAWSWYKVTTLLQTAGHNVTALDMAAAGINQTQP-ESLTSLTEYYEPLLS 95
           HFVLVHG+C GAW WYKV  LL+  GH VTA+D+AA+GI+ T+    + +  +Y EPL  
Sbjct: 9   HFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYSEPLTK 68

Query: 96  FMEALPSDEKIVLVGHSLGGLGISMAMERFPEKISVAIFVTAAMPG-PIGGIFTIDELKG 155
            + +LP+DEK+VLVGHS GGL +++AME+FPEKISVA+F+TA MP       F +D+   
Sbjct: 69  LLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVLDKFGS 128

Query: 156 IL--EMFRAFYSEESKASDSNL-FLFPKEELETKLFPLSPPEDLTLATTLVRPQAMF-GM 215
            +  E +     E   + +S L   F  + ++  L+ LSP EDL L   L+RP ++F   
Sbjct: 129 NMPQEAWMGTEFEPYGSDNSGLSMFFSPDFMKLGLYQLSPVEDLELGLLLMRPGSLFIND 188

Query: 216 LESMKELALSRDKYGSVKRAFIISEKDLMTPKILVWAMLTFNNPDLQAQIQGSDHMVMAS 275
           L  MK    S + YGSV R FI+ ++D   P+     M+     +L  +++ +DHM M  
Sbjct: 189 LSKMKN--FSDEGYGSVPRVFIVCKEDKAIPEERQRWMIDNFPVNLVMEMEETDHMPMFC 248

Query: 276 KPLQLAQQLATIAQHF 286
           KP QL+     IA  F
Sbjct: 249 KPQQLSDYFLKIADKF 262

[Close]

BLAST of Tan0012012 vs. ExPASy Swiss-Prot
Match: O80476 (Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 4.0e-42
Identity = 103/255 (40.39%), Postives = 151/255 (59.22%), Query Frame = 0


Query: 36  HFVLVHGACLGAWSWYKVTTLLQTAGHNVTALDMAAAGINQTQP-ESLTSLTEYYEPLLS 95
           HFVLVHGAC GAW WYKV  LL+  GH VTALD+AA+GI+ T+    +++  +Y EPL+ 
Sbjct: 9   HFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEPLMQ 68

Query: 96  FMEALPSDEKIVLVGHSLGGLGISMAMERFPEKISVAIFVTAAMPGPIGGIFTIDELKGI 155
            M +LP+DEK+VLVGHS GGL +++AM++FP+KISV++FVTA MP        ++E    
Sbjct: 69  LMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEKFAS 128

Query: 156 LEMFRAFYSEESKASDSN----LFLFPKEELETKLFPLSPPEDLTLATTLVRPQAMFGML 215
                 +   E +   S+       F  + ++ +L+ LSP EDL L   L RP ++F + 
Sbjct: 129 SMTPEGWMGSELETYGSDNSGLSVFFSTDFMKHRLYQLSPVEDLELGLLLKRPSSLF-IN 188

Query: 216 ESMKELALSRDKYGSVKRAFIISEKDLMTPKILVWAMLTFNNPDLQAQIQGSDHMVMASK 275
           E  K    S   YGSV RA+I+ ++D +  +     M+     +L  +++ +DHM M  K
Sbjct: 189 ELSKMENFSEKGYGSVPRAYIVCKEDNIISEDHQRWMIHNYPANLVIEMEETDHMPMFCK 248

Query: 276 PLQLAQQLATIAQHF 286
           P  L+  L  IA +F
Sbjct: 249 PQLLSDHLLAIADNF 262

[Close]

BLAST of Tan0012012 vs. NCBI nr
Match: XP_022988863.1 (probable esterase PIR7A [Cucurbita maxima])

HSP 1 Score: 452.2 bits (1162), Expect = 3.4e-123
Identity = 243/291 (83.51%), Postives = 260/291 (89.35%), Query Frame = 0


Query: 1   MQKFLLPFLFLIFLSFSSLTRSSILLHDHSQSSTSHFVLVHGACLGAWSWYKVTTLLQTA 60
           MQK LLPFL LI +SFSS T SS    D S+S+ SHFVLVHGACLGAWSWYKV TLLQTA
Sbjct: 10  MQKLLLPFLVLILVSFSSPTWSS----DQSESTYSHFVLVHGACLGAWSWYKVITLLQTA 69

Query: 61  GHNVTALDMAAAGINQTQPESLTSLTEYYEPLLSFMEALPSDEKIVLVGHSLGGLGISMA 120
           GH VTALDMAAAGINQTQPESL SLTEY+EPLLSFMEALPSDE+IVLVGHSLGGLGISMA
Sbjct: 70  GHKVTALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMA 129

Query: 121 MERFPEKISVAIFVTAAMPGPIGGIFTIDELKGILEMFRAFYSEE-SKASDSNLFLFPKE 180
           MERFPEK+SVAIFVTAAMPGPI G FTI++L  IL+M RAFYSEE SKAS+SNLFLF +E
Sbjct: 130 MERFPEKVSVAIFVTAAMPGPIVGSFTIEKLMKILQMLRAFYSEEDSKASNSNLFLFSQE 189

Query: 181 ELETKLFPLSPPEDLTLATTLVRPQAMFGMLESMKELALSRDKYGSVKRAFIISEKDLMT 240
           ELE KLFPLSPPEDLTLATTLVRPQAMFG+LESMKEL L+RDKYGSVKRAFIISEKD MT
Sbjct: 190 ELEAKLFPLSPPEDLTLATTLVRPQAMFGILESMKELRLTRDKYGSVKRAFIISEKDEMT 249

Query: 241 PKILVWAMLTFNNPDLQAQIQGSDHMVMASKPLQLAQQLATIAQHFASSSS 291
             I+VWAMLTFN PD QA ++GSDHMVMASKPLQLAQQL TIAQ FASSS+
Sbjct: 250 SMIMVWAMLTFNKPDRQAVVKGSDHMVMASKPLQLAQQLTTIAQDFASSST 296

[Close]

BLAST of Tan0012012 vs. NCBI nr
Match: XP_022931632.1 (methylesterase 2-like [Cucurbita moschata])

HSP 1 Score: 448.7 bits (1153), Expect = 3.7e-122
Identity = 240/291 (82.47%), Postives = 259/291 (89.00%), Query Frame = 0


Query: 1   MQKFLLPFLFLIFLSFSSLTRSSILLHDHSQSSTSHFVLVHGACLGAWSWYKVTTLLQTA 60
           MQK LL FL LI + FSS TRSS    D S+S+ SHFVLVHGACLGAWSWYKV TLLQTA
Sbjct: 1   MQKLLLSFLVLILVLFSSPTRSS----DQSESTYSHFVLVHGACLGAWSWYKVITLLQTA 60

Query: 61  GHNVTALDMAAAGINQTQPESLTSLTEYYEPLLSFMEALPSDEKIVLVGHSLGGLGISMA 120
           GH VTALDMAAAGINQTQPESL SLTEY+EPLLSFMEALPSDE+IVLVGHSLGGLGISMA
Sbjct: 61  GHKVTALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMA 120

Query: 121 MERFPEKISVAIFVTAAMPGPIGGIFTIDELKGILEMFRAFYSEE-SKASDSNLFLFPKE 180
           ME FPEK+SVAIFVTAAMPGPI G FTI+EL  ILEM RAFYSEE SKASDSNLFLF +E
Sbjct: 121 MESFPEKVSVAIFVTAAMPGPIVGSFTIEELMKILEMLRAFYSEEDSKASDSNLFLFSQE 180

Query: 181 ELETKLFPLSPPEDLTLATTLVRPQAMFGMLESMKELALSRDKYGSVKRAFIISEKDLMT 240
           EL+TKLFPLSPP+D+ LATTL RPQAMFG+LESMKEL L+RDKYGSVKRAFIISEKD MT
Sbjct: 181 ELKTKLFPLSPPQDVALATTLERPQAMFGILESMKELRLTRDKYGSVKRAFIISEKDEMT 240

Query: 241 PKILVWAMLTFNNPDLQAQIQGSDHMVMASKPLQLAQQLATIAQHFASSSS 291
            KI+VWAMLTFN PD QA+++GSDHMVMASKPL+LAQQL TIAQ FASSS+
Sbjct: 241 SKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASSST 287

[Close]

BLAST of Tan0012012 vs. NCBI nr
Match: KAG6588637.1 (Methylesterase 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 448.4 bits (1152), Expect = 4.9e-122
Identity = 239/291 (82.13%), Postives = 259/291 (89.00%), Query Frame = 0


Query: 1   MQKFLLPFLFLIFLSFSSLTRSSILLHDHSQSSTSHFVLVHGACLGAWSWYKVTTLLQTA 60
           MQK LL FL LI + FSS TRSS    D S+S+ SHFVLVHGACLGAWSWYKV TLLQTA
Sbjct: 1   MQKLLLSFLVLILVLFSSPTRSS----DQSESTYSHFVLVHGACLGAWSWYKVITLLQTA 60

Query: 61  GHNVTALDMAAAGINQTQPESLTSLTEYYEPLLSFMEALPSDEKIVLVGHSLGGLGISMA 120
           GH VTALDMAAAGINQTQPESL SLTEY+EPLLSFMEALPSDE+IVLVGHSLGGLGISMA
Sbjct: 61  GHKVTALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMA 120

Query: 121 MERFPEKISVAIFVTAAMPGPIGGIFTIDELKGILEMFRAFYSEE-SKASDSNLFLFPKE 180
           MERFPEK+SVAIFVTAAMPGPI G FT++EL  ILEM RAFYSEE SKASDS LFLF +E
Sbjct: 121 MERFPEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLFLFSQE 180

Query: 181 ELETKLFPLSPPEDLTLATTLVRPQAMFGMLESMKELALSRDKYGSVKRAFIISEKDLMT 240
           EL+TKLFPLSPP+D+ LATTL RPQAMFG+LESMKEL L+RDKYGSVKRAFIISEKD MT
Sbjct: 181 ELKTKLFPLSPPQDVALATTLERPQAMFGILESMKELRLTRDKYGSVKRAFIISEKDEMT 240

Query: 241 PKILVWAMLTFNNPDLQAQIQGSDHMVMASKPLQLAQQLATIAQHFASSSS 291
            KI+VWAMLTFN PD QA+++GSDHMVMASKPL+LAQQL TIAQ FASSS+
Sbjct: 241 SKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASSST 287

[Close]

BLAST of Tan0012012 vs. NCBI nr
Match: XP_023530119.1 (methylesterase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 444.5 bits (1142), Expect = 7.1e-121
Identity = 236/290 (81.38%), Postives = 256/290 (88.28%), Query Frame = 0


Query: 1   MQKFLLPFLFLIFLSFSSLTRSSILLHDHSQSSTSHFVLVHGACLGAWSWYKVTTLLQTA 60
           M K LLPFL LI +SFSS TRSS    D S+ + SHFVLVHGACLGAWSWYKVTT LQTA
Sbjct: 1   MHKLLLPFLVLIVVSFSSPTRSS----DQSELTYSHFVLVHGACLGAWSWYKVTTFLQTA 60

Query: 61  GHNVTALDMAAAGINQTQPESLTSLTEYYEPLLSFMEALPSDEKIVLVGHSLGGLGISMA 120
           GH VTALDMAAAGINQTQPESL SLTEY+EPLLSFME LPSDE++VLVGHSLGGLGISMA
Sbjct: 61  GHKVTALDMAAAGINQTQPESLASLTEYFEPLLSFMEVLPSDERVVLVGHSLGGLGISMA 120

Query: 121 MERFPEKISVAIFVTAAMPGPIGGIFTIDELKGILEMFRAFYSEE-SKASDSNLFLFPKE 180
           MERFPEK+SVAIFVTAAMPGPI G FTI+ L  ILE  R FYSEE SKAS+SNLFLF +E
Sbjct: 121 MERFPEKVSVAIFVTAAMPGPIVGSFTIEHLMKILETLRGFYSEEDSKASNSNLFLFSQE 180

Query: 181 ELETKLFPLSPPEDLTLATTLVRPQAMFGMLESMKELALSRDKYGSVKRAFIISEKDLMT 240
           ELETKLFPLSPP+D+ LATTLVRPQAMFG+LESMKEL L+R+KYGSVKRAFIISEKD MT
Sbjct: 181 ELETKLFPLSPPKDVALATTLVRPQAMFGILESMKELRLTREKYGSVKRAFIISEKDEMT 240

Query: 241 PKILVWAMLTFNNPDLQAQIQGSDHMVMASKPLQLAQQLATIAQHFASSS 290
            KI+VWAMLTFN PD +A+++GSDHMVMASKPLQLAQQL TIAQ FASSS
Sbjct: 241 SKIMVWAMLTFNKPDREAEVKGSDHMVMASKPLQLAQQLTTIAQDFASSS 286

[Close]

BLAST of Tan0012012 vs. NCBI nr
Match: XP_038887398.1 (probable esterase PIR7A [Benincasa hispida])

HSP 1 Score: 439.1 bits (1128), Expect = 3.0e-119
Identity = 233/294 (79.25%), Postives = 258/294 (87.76%), Query Frame = 0


Query: 1   MQKFLLPFLFLIFLSFSSLTRSSILLHDHSQSSTSHFVLVHGACLGAWSWYKVTTLLQTA 60
           MQK LL F  LIFL F S T+SS +LHD SQ S SH VLVHGACLGAWSWYKVTTLLQTA
Sbjct: 1   MQKLLLLFFLLIFLCFCSQTQSSSVLHDDSQGSKSHIVLVHGACLGAWSWYKVTTLLQTA 60

Query: 61  GHNVTALDMAAAGINQTQPESLTSLTEYYEPLLSFMEALPSDEKIVLVGHSLGGLGISMA 120
           GHNVTALDMAA+GIN TQ ESLTSLTEYY+PLLSFMEAL  DEKIVLVGHSLGGLGISMA
Sbjct: 61  GHNVTALDMAASGINPTQAESLTSLTEYYQPLLSFMEALAPDEKIVLVGHSLGGLGISMA 120

Query: 121 MERFPEKISVAIFVTAAMPGPIGGIFTIDELKGILEMFRAFYSEE-SKASDSNLFLFPKE 180
           MERFPEKISVAIFV+AAMPGPI G+ +I+E K ILEM+ AFY+EE SKAS+ +LFLF +E
Sbjct: 121 MERFPEKISVAIFVSAAMPGPILGLSSIEEQKKILEMYSAFYTEEKSKASNPDLFLFSQE 180

Query: 181 ELETKLFPLSPPEDLTLATTLVRPQAMFGMLESMKELALSRDKYGSVKRAFIISEKDLMT 240
           +LETKLFPLSPPEDLTLATTLVRPQAMFG+LESMKEL LS++KYGSVKRAFIISEKD MT
Sbjct: 181 DLETKLFPLSPPEDLTLATTLVRPQAMFGLLESMKELQLSQEKYGSVKRAFIISEKDQMT 240

Query: 241 PKILVWAMLTFNNPDLQAQIQGSDHMVMASKPLQLAQQLATIAQHFASSSSSYN 294
            KI+VWAML FN PD  A+++GSDHMVM SKPL+L QQL+TIAQ F+SSS+  N
Sbjct: 241 TKIMVWAMLIFNKPDRVAEVKGSDHMVMTSKPLELTQQLSTIAQDFSSSSTYIN 294

[Close]

BLAST of Tan0012012 vs. ExPASy TrEMBL
Match: A0A6J1JNJ9 (probable esterase PIR7A OS=Cucurbita maxima OX=3661 GN=LOC111486088 PE=4 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.6e-123
Identity = 243/291 (83.51%), Postives = 260/291 (89.35%), Query Frame = 0


Query: 1   MQKFLLPFLFLIFLSFSSLTRSSILLHDHSQSSTSHFVLVHGACLGAWSWYKVTTLLQTA 60
           MQK LLPFL LI +SFSS T SS    D S+S+ SHFVLVHGACLGAWSWYKV TLLQTA
Sbjct: 10  MQKLLLPFLVLILVSFSSPTWSS----DQSESTYSHFVLVHGACLGAWSWYKVITLLQTA 69

Query: 61  GHNVTALDMAAAGINQTQPESLTSLTEYYEPLLSFMEALPSDEKIVLVGHSLGGLGISMA 120
           GH VTALDMAAAGINQTQPESL SLTEY+EPLLSFMEALPSDE+IVLVGHSLGGLGISMA
Sbjct: 70  GHKVTALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMA 129

Query: 121 MERFPEKISVAIFVTAAMPGPIGGIFTIDELKGILEMFRAFYSEE-SKASDSNLFLFPKE 180
           MERFPEK+SVAIFVTAAMPGPI G FTI++L  IL+M RAFYSEE SKAS+SNLFLF +E
Sbjct: 130 MERFPEKVSVAIFVTAAMPGPIVGSFTIEKLMKILQMLRAFYSEEDSKASNSNLFLFSQE 189

Query: 181 ELETKLFPLSPPEDLTLATTLVRPQAMFGMLESMKELALSRDKYGSVKRAFIISEKDLMT 240
           ELE KLFPLSPPEDLTLATTLVRPQAMFG+LESMKEL L+RDKYGSVKRAFIISEKD MT
Sbjct: 190 ELEAKLFPLSPPEDLTLATTLVRPQAMFGILESMKELRLTRDKYGSVKRAFIISEKDEMT 249

Query: 241 PKILVWAMLTFNNPDLQAQIQGSDHMVMASKPLQLAQQLATIAQHFASSSS 291
             I+VWAMLTFN PD QA ++GSDHMVMASKPLQLAQQL TIAQ FASSS+
Sbjct: 250 SMIMVWAMLTFNKPDRQAVVKGSDHMVMASKPLQLAQQLTTIAQDFASSST 296

[Close]

BLAST of Tan0012012 vs. ExPASy TrEMBL
Match: A0A6J1EU76 (methylesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111437792 PE=4 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 1.8e-122
Identity = 240/291 (82.47%), Postives = 259/291 (89.00%), Query Frame = 0


Query: 1   MQKFLLPFLFLIFLSFSSLTRSSILLHDHSQSSTSHFVLVHGACLGAWSWYKVTTLLQTA 60
           MQK LL FL LI + FSS TRSS    D S+S+ SHFVLVHGACLGAWSWYKV TLLQTA
Sbjct: 1   MQKLLLSFLVLILVLFSSPTRSS----DQSESTYSHFVLVHGACLGAWSWYKVITLLQTA 60

Query: 61  GHNVTALDMAAAGINQTQPESLTSLTEYYEPLLSFMEALPSDEKIVLVGHSLGGLGISMA 120
           GH VTALDMAAAGINQTQPESL SLTEY+EPLLSFMEALPSDE+IVLVGHSLGGLGISMA
Sbjct: 61  GHKVTALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMA 120

Query: 121 MERFPEKISVAIFVTAAMPGPIGGIFTIDELKGILEMFRAFYSEE-SKASDSNLFLFPKE 180
           ME FPEK+SVAIFVTAAMPGPI G FTI+EL  ILEM RAFYSEE SKASDSNLFLF +E
Sbjct: 121 MESFPEKVSVAIFVTAAMPGPIVGSFTIEELMKILEMLRAFYSEEDSKASDSNLFLFSQE 180

Query: 181 ELETKLFPLSPPEDLTLATTLVRPQAMFGMLESMKELALSRDKYGSVKRAFIISEKDLMT 240
           EL+TKLFPLSPP+D+ LATTL RPQAMFG+LESMKEL L+RDKYGSVKRAFIISEKD MT
Sbjct: 181 ELKTKLFPLSPPQDVALATTLERPQAMFGILESMKELRLTRDKYGSVKRAFIISEKDEMT 240

Query: 241 PKILVWAMLTFNNPDLQAQIQGSDHMVMASKPLQLAQQLATIAQHFASSSS 291
            KI+VWAMLTFN PD QA+++GSDHMVMASKPL+LAQQL TIAQ FASSS+
Sbjct: 241 SKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASSST 287

[Close]

BLAST of Tan0012012 vs. ExPASy TrEMBL
Match: A0A1S3C0U9 (methylesterase 2-like OS=Cucumis melo OX=3656 GN=LOC103495205 PE=4 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 5.1e-109
Identity = 215/298 (72.15%), Postives = 254/298 (85.23%), Query Frame = 0


Query: 1   MQKFLLP-FLFLIFLSFSSLTRSS--ILLHDHSQSSTSHFVLVHGACLGAWSWYKVTTLL 60
           MQK LLP F  LIFLSFS+ T++S  +L H +SQS+ SHFVLVHGACLGAWSWYKVTT L
Sbjct: 1   MQKLLLPLFFLLIFLSFSTQTQTSFDLLHHHYSQSTKSHFVLVHGACLGAWSWYKVTTFL 60

Query: 61  QTAGHNVTALDMAAAGINQTQPESLTSLTEYYEPLLSFMEALPSDEKIVLVGHSLGGLGI 120
           QTAGHNVTALDMAAAGI+ TQP+SL ++T+Y++PLL+FMEAL +DEKIVLVGHSLGGLGI
Sbjct: 61  QTAGHNVTALDMAAAGIDPTQPQSLKTITDYFQPLLNFMEALQADEKIVLVGHSLGGLGI 120

Query: 121 SMAMERFPEKISVAIFVTAAMPGPIGGIFTIDELKGILEMFRAFYSEE---SKASD-SNL 180
           SMAMERFPEKISVAIFVTAAMPGPI  +F+++E + +L M+  FY+EE   SK SD SNL
Sbjct: 121 SMAMERFPEKISVAIFVTAAMPGPIPWLFSMEEQRKVLGMYGGFYTEEDFKSKGSDNSNL 180

Query: 181 FLFPKEELETKLFPLSPPEDLTLATTLVRPQAMFGMLESMKELALSRDKYGSVKRAFIIS 240
           F+F +EELETKLFPLSPPEDLTLA TLVRPQAMFG+ ES+KEL LS++ YGSVKRAFIIS
Sbjct: 181 FMFSEEELETKLFPLSPPEDLTLAKTLVRPQAMFGLFESVKELKLSKENYGSVKRAFIIS 240

Query: 241 EKDLMTPKILVWAMLTFNNPDLQAQIQGSDHMVMASKPLQLAQQLATIAQHFASSSSS 292
           EKD M  KI+VWAM+  N P    ++ GSDHMVM SKPL+LA+QL+TIAQ FA+++SS
Sbjct: 241 EKDKMATKIMVWAMIISNRPHHVGEVHGSDHMVMTSKPLELARQLSTIAQDFAAAASS 298

[Close]

BLAST of Tan0012012 vs. ExPASy TrEMBL
Match: A0A0A0K4V8 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G074970 PE=4 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 1.2e-105
Identity = 210/304 (69.08%), Postives = 243/304 (79.93%), Query Frame = 0


Query: 1   MQKFLLPFLFLIFLSFSSLTRSSILLH--------DHSQSSTSHFVLVHGACLGAWSWYK 60
           MQK LLPF  LIFLSFS+ T++S L H          SQS+ SHFVLVHGACLGAWSWY+
Sbjct: 1   MQKLLLPFFLLIFLSFSTQTQTSFLFHHGHHDNDGHDSQSNKSHFVLVHGACLGAWSWYQ 60

Query: 61  VTTLLQTAGHNVTALDMAAAGINQTQPESLTSLTEYYEPLLSFMEALPSDEKIVLVGHSL 120
           VTT LQTAGH VTA+DMAAAGI+ TQPESLTSLT+Y++PLL+F EAL +D+KIVLVGHSL
Sbjct: 61  VTTFLQTAGHKVTAVDMAAAGIDPTQPESLTSLTDYFQPLLNFTEALQADDKIVLVGHSL 120

Query: 121 GGLGISMAMERFPEKISVAIFVTAAMPGPIGGIFTIDELKGILEMFRAFYSEESKASD-- 180
           GGLGISMAMERFPEKISVAIFVTAAMPGPI G  +I+E K  L M++AF+  +   S+  
Sbjct: 121 GGLGISMAMERFPEKISVAIFVTAAMPGPIIGFQSIEEQKTTLGMYKAFHKGDDTKSEVS 180

Query: 181 ---SNLFLFPKEELETKLFPLSPPEDLTLATTLVRPQAMFGMLESMKELALSRDKYGSVK 240
                LF+F +EELETKLFPLSPP+DLTLA TLVRPQAMFG+LESMKEL LS++ YGSVK
Sbjct: 181 KKPPRLFMFSEEELETKLFPLSPPQDLTLARTLVRPQAMFGLLESMKELRLSKENYGSVK 240

Query: 241 RAFIISEKDLMTPKILVWAMLTFNNPDLQAQIQGSDHMVMASKPLQLAQQLATIAQHFAS 292
           RAFIIS+ D MT K +VWAML  N PD   ++ GSDHMVM SKPL+LAQ L TIAQ +A+
Sbjct: 241 RAFIISQNDKMTSKFMVWAMLLLNKPDRVEEVHGSDHMVMTSKPLELAQLLGTIAQDYAA 300

[Close]

BLAST of Tan0012012 vs. ExPASy TrEMBL
Match: A0A6J1C2S8 (methylesterase 3-like OS=Momordica charantia OX=3673 GN=LOC111007844 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 1.2e-102
Identity = 213/293 (72.70%), Postives = 238/293 (81.23%), Query Frame = 0


Query: 5   LLPF-LFLIFLSFSSLTRSSIL-LHDHSQSSTS--HFVLVHGACLGAWSWYKVTTLLQTA 64
           LLPF L LI LS SS T+SS L LH   QSS+   HFVLVHGACLGAWSWYKVTT LQ A
Sbjct: 18  LLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGACLGAWSWYKVTTYLQLA 77

Query: 65  GHNVTALDMAAAGINQTQPESLTSLTEYYEPLLSFMEALPSDEKIVLVGHSLGGLGISMA 124
           GHNVTALDMA AGIN TQP+SL+SLT+YYEPLL FM+ALPSDEK+VLVGHSLGGLGISMA
Sbjct: 78  GHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDEKVVLVGHSLGGLGISMA 137

Query: 125 MERFPEKISVAIFVTAAMPGPIGGIFTIDELKGILEMFRAFY---SEESKASDSNLFLFP 184
           ME FPEKISVAIFVTAAMPGPI     ++E K ILEM+  F     E SK SD+ LF+F 
Sbjct: 138 MEEFPEKISVAIFVTAAMPGPIS---NLEEKKKILEMYGTFNKKDEERSKVSDAKLFIFT 197

Query: 185 KEELETKLFPLSPPEDLTLATTLVRPQAMFGMLESMKELALSRDKYGSVKRAFIISEKDL 244
           +E+L TKLFPLSPPEDL LATTLVRPQ MF +++SM+EL L+RD YGSVKRAFIISE D 
Sbjct: 198 EEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHYGSVKRAFIISEDDE 257

Query: 245 MTPKILVWAMLTFNNPDLQAQIQGSDHMVMASKPLQLAQQLATIAQHFASSSS 291
           M  +I+VWAML  N PD  A+I+GSDHMVMASKPL LAQQL+TIA  FASS+S
Sbjct: 258 MALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIALEFASSTS 307

[Close]

BLAST of Tan0012012 vs. TAIR 10
Match: AT2G23610.1 (methyl esterase 3 )

HSP 1 Score: 184.9 bits (468), Expect = 9.4e-47
Identity = 109/256 (42.58%), Postives = 153/256 (59.77%), Query Frame = 0


Query: 36  HFVLVHGACLGAWSWYKVTTLLQTAGHNVTALDMAAAGINQTQP-ESLTSLTEYYEPLLS 95
           H VLVHGAC GAW WYKV   L+ +GH VTA+D+AA+GI+ T+    +++  +Y EPL+ 
Sbjct: 9   HVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYSEPLMQ 68

Query: 96  FMEALPSDEKIVLVGHSLGGLGISMAMERFPEKISVAIFVTAAMPG-PIGGIFTIDELK- 155
            M +LP DEK+VLVGHSLGGL ++MAM+ FP KISV++FVTA MP       F  D+L+ 
Sbjct: 69  LMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSFVWDKLRK 128

Query: 156 --GILEMFRAFYSEESKASDSNLFLFPKEELETKLFPLSPPEDLTLATTLVRPQAMFGML 215
                E     ++ E     S  ++F  E +   L+ LSP +DL LA  LVR   +    
Sbjct: 129 ETSREEWLDTVFTSEKPDFPSEFWIFGPEFMAKNLYQLSPVQDLELAKMLVRANPLI-KK 188

Query: 216 ESMKELALSRDKYGSVKRAFIISEKDLMTPKILVWAMLTFNNPDLQAQIQGSDHMVMASK 275
           +  +  + S + YGSV R FI+  KDL++P+    +M++   P    +I+ +DHM M SK
Sbjct: 189 DMAERRSFSEEGYGSVTRIFIVCGKDLVSPEDYQRSMISNFPPKEVMEIKDADHMPMFSK 248

Query: 276 PLQLAQQLATIAQHFA 287
           P QL   L  IA  +A
Sbjct: 249 PQQLCALLLEIANKYA 263

[Close]

BLAST of Tan0012012 vs. TAIR 10
Match: AT2G23590.1 (methyl esterase 8 )

HSP 1 Score: 179.1 bits (453), Expect = 5.2e-45
Identity = 105/254 (41.34%), Postives = 151/254 (59.45%), Query Frame = 0


Query: 36  HFVLVHGACLGAWSWYKVTTLLQTAGHNVTALDMAAAGINQTQPESLTSLTEYYEPLLSF 95
           HFVLVHG+CLGAW WYKV  LL+ +GH VTALD+AA GI+      +++  +Y EPL+  
Sbjct: 27  HFVLVHGSCLGAWCWYKVKPLLEASGHRVTALDLAACGIDTRSITDISTCEQYSEPLIQL 86

Query: 96  MEALPSDEKIVLVGHSLGGLGISMAMERFPEKISVAIFVTAAMPGPIGGIFTIDELKGIL 155
           M +LP+DEK+VLVGHS GGL +++AM++FP+KISV++FVT+ MP       T +    +L
Sbjct: 87  MTSLPNDEKVVLVGHSYGGLTLAIAMDKFPDKISVSVFVTSFMPD------TKNSPSFVL 146

Query: 156 EMFRAFYSEESKASD--SNLFLFPKEELETKLFPLSPPEDLTLATTLVRPQAMF-GMLES 215
           E F +  + E           +F  E  + ++  LSP EDL L   L RP ++F   L  
Sbjct: 147 EKFASTMTPEDWMGSELEPYVVFSAEFTKHRILQLSPIEDLELRLLLKRPGSLFLNDLSR 206

Query: 216 MKELALSRDKYGSVKRAFIISEKDLMTPKILVWAMLTFNNPDLQAQIQGSDHMVMASKPL 275
           MK    S   YGSV RA+I+S+ D    +     M+    P+L  +++G+DH+ +  KP 
Sbjct: 207 MKN--FSEKGYGSVPRAYIVSKDDHTISEEYQRWMIDNYPPNLVIEMEGTDHLPLFCKPQ 266

Query: 276 QLAQQLATIAQHFA 287
            L+  L  IA  F+
Sbjct: 267 LLSDHLLAIADKFS 272

[Close]

BLAST of Tan0012012 vs. TAIR 10
Match: AT2G23620.1 (methyl esterase 1 )

HSP 1 Score: 174.5 bits (441), Expect = 1.3e-43
Identity = 107/256 (41.80%), Postives = 152/256 (59.38%), Query Frame = 0


Query: 36  HFVLVHGACLGAWSWYKVTTLLQTAGHNVTALDMAAAGINQTQP-ESLTSLTEYYEPLLS 95
           HFVLVHG+C GAW WYKV  LL+  GH VTA+D+AA+GI+ T+    + +  +Y EPL  
Sbjct: 9   HFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYSEPLTK 68

Query: 96  FMEALPSDEKIVLVGHSLGGLGISMAMERFPEKISVAIFVTAAMPG-PIGGIFTIDELKG 155
            + +LP+DEK+VLVGHS GGL +++AME+FPEKISVA+F+TA MP       F +D+   
Sbjct: 69  LLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVLDKFGS 128

Query: 156 IL--EMFRAFYSEESKASDSNL-FLFPKEELETKLFPLSPPEDLTLATTLVRPQAMF-GM 215
            +  E +     E   + +S L   F  + ++  L+ LSP EDL L   L+RP ++F   
Sbjct: 129 NMPQEAWMGTEFEPYGSDNSGLSMFFSPDFMKLGLYQLSPVEDLELGLLLMRPGSLFIND 188

Query: 216 LESMKELALSRDKYGSVKRAFIISEKDLMTPKILVWAMLTFNNPDLQAQIQGSDHMVMAS 275
           L  MK    S + YGSV R FI+ ++D   P+     M+     +L  +++ +DHM M  
Sbjct: 189 LSKMKN--FSDEGYGSVPRVFIVCKEDKAIPEERQRWMIDNFPVNLVMEMEETDHMPMFC 248

Query: 276 KPLQLAQQLATIAQHF 286
           KP QL+     IA  F
Sbjct: 249 KPQQLSDYFLKIADKF 262

[Close]

BLAST of Tan0012012 vs. TAIR 10
Match: AT2G23600.1 (acetone-cyanohydrin lyase )

HSP 1 Score: 173.3 bits (438), Expect = 2.8e-43
Identity = 103/255 (40.39%), Postives = 151/255 (59.22%), Query Frame = 0


Query: 36  HFVLVHGACLGAWSWYKVTTLLQTAGHNVTALDMAAAGINQTQP-ESLTSLTEYYEPLLS 95
           HFVLVHGAC GAW WYKV  LL+  GH VTALD+AA+GI+ T+    +++  +Y EPL+ 
Sbjct: 9   HFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEPLMQ 68

Query: 96  FMEALPSDEKIVLVGHSLGGLGISMAMERFPEKISVAIFVTAAMPGPIGGIFTIDELKGI 155
            M +LP+DEK+VLVGHS GGL +++AM++FP+KISV++FVTA MP        ++E    
Sbjct: 69  LMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEKFAS 128

Query: 156 LEMFRAFYSEESKASDSN----LFLFPKEELETKLFPLSPPEDLTLATTLVRPQAMFGML 215
                 +   E +   S+       F  + ++ +L+ LSP EDL L   L RP ++F + 
Sbjct: 129 SMTPEGWMGSELETYGSDNSGLSVFFSTDFMKHRLYQLSPVEDLELGLLLKRPSSLF-IN 188

Query: 216 ESMKELALSRDKYGSVKRAFIISEKDLMTPKILVWAMLTFNNPDLQAQIQGSDHMVMASK 275
           E  K    S   YGSV RA+I+ ++D +  +     M+     +L  +++ +DHM M  K
Sbjct: 189 ELSKMENFSEKGYGSVPRAYIVCKEDNIISEDHQRWMIHNYPANLVIEMEETDHMPMFCK 248

Query: 276 PLQLAQQLATIAQHF 286
           P  L+  L  IA +F
Sbjct: 249 PQLLSDHLLAIADNF 262

[Close]

BLAST of Tan0012012 vs. TAIR 10
Match: AT3G50440.1 (methyl esterase 10 )

HSP 1 Score: 171.4 bits (433), Expect = 1.1e-42
Identity = 104/266 (39.10%), Postives = 150/266 (56.39%), Query Frame = 0


Query: 27  HDHSQSSTSHFVLVHGACLGAWSWYKVTTLLQTAGHNVTALDMAAAGINQTQPESLTSLT 86
           H   Q    HFV VHG+C GAW W+K+   L+  GH VTA+D+  +G++  Q   +  ++
Sbjct: 25  HHMQQQQLHHFVFVHGSCHGAWCWFKLAAKLKLDGHRVTAIDLGGSGVDTRQLHEVRLVS 84

Query: 87  EYYEPLLSFMEALPSDEKIVLVGHSLGGLGISMAMERFPEKISVAIFVTAAMP---GP-- 146
            Y EPL+SFME+LP +EK+VLVGHS GG+G S+AMERFP K+SV IF++A MP    P  
Sbjct: 85  AYLEPLMSFMESLPENEKVVLVGHSYGGIGTSLAMERFPTKVSVGIFLSAYMPHHDSPPA 144

Query: 147 --IGGIFTIDELKGILEMFRAFYSEESKASDSNLFLFPKEELETKLFPLSPPEDLTLATT 206
             I   FT   L     M   F  EE      +  LF    L+ K +     EDL LA  
Sbjct: 145 VLIQEYFT--RLPEGFAMDCEFTFEEGLEHPPSSVLFGTSFLKEKAYSNCQLEDLELAMA 204

Query: 207 LVRPQAMFGMLESMKELALSRDKYGSVKRAFIISEKDLMTPKILVWAMLTFNNPDLQAQI 266
           L++P  ++   E   E  +++++YGS KR FI+ E D + P+ +   M++   P    +I
Sbjct: 205 LMKPSWLY-TKEMGGEDLITKERYGSGKRVFIVCEGDNVVPEEIQKWMISNYEPHEVKRI 264

Query: 267 QGSDHMVMASKPLQLAQQLATIAQHF 286
           + + HM M +KP +L+Q L  IA  +
Sbjct: 265 EEAGHMAMLTKPHELSQLLQEIAAKY 287

The following BLAST results are available for this feature:

BLAST of Tan0012012 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80477	1.3e-45	42.58	Methylesterase 3 OS=Arabidopsis thaliana OX=3702 GN=MES3 PE=2 SV=1	[more]
O80475	7.3e-44	41.34	Methylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=MES8 PE=3 SV=1	[more]
Q6RYA0	1.0e-42	39.53	Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1	[more]
Q8S8S9	1.8e-42	41.80	Methylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=MES1 PE=1 SV=1	[more]
O80476	4.0e-42	40.39	Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1	[more]

BLAST of Tan0012012 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022988863.1	3.4e-123	83.51	probable esterase PIR7A [Cucurbita maxima]	[more]
XP_022931632.1	3.7e-122	82.47	methylesterase 2-like [Cucurbita moschata]	[more]
KAG6588637.1	4.9e-122	82.13	Methylesterase 8, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023530119.1	7.1e-121	81.38	methylesterase 3-like [Cucurbita pepo subsp. pepo]	[more]
XP_038887398.1	3.0e-119	79.25	probable esterase PIR7A [Benincasa hispida]	[more]

BLAST of Tan0012012 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JNJ9	1.6e-123	83.51	probable esterase PIR7A OS=Cucurbita maxima OX=3661 GN=LOC111486088 PE=4 SV=1	[more]
A0A6J1EU76	1.8e-122	82.47	methylesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111437792 PE=4 SV=1	[more]
A0A1S3C0U9	5.1e-109	72.15	methylesterase 2-like OS=Cucumis melo OX=3656 GN=LOC103495205 PE=4 SV=1	[more]
A0A0A0K4V8	1.2e-105	69.08	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G074...	[more]
A0A6J1C2S8	1.2e-102	72.70	methylesterase 3-like OS=Momordica charantia OX=3673 GN=LOC111007844 PE=4 SV=1	[more]

BLAST of Tan0012012 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23610.1	9.4e-47	42.58	methyl esterase 3	[more]
AT2G23590.1	5.2e-45	41.34	methyl esterase 8	[more]
AT2G23620.1	1.3e-43	41.80	methyl esterase 1	[more]
AT2G23600.1	2.8e-43	40.39	acetone-cyanohydrin lyase	[more]
AT3G50440.1	1.1e-42	39.10	methyl esterase 10	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF12697	Abhydrolase_6	coord: 37..276 e-value: 9.7E-15 score: 55.8
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 33..286 e-value: 2.0E-54 score: 187.1
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 32..280
None	No IPR available	PANTHER	PTHR10992	METHYLESTERASE FAMILY MEMBER	coord: 24..284
None	No IPR available	PANTHER	PTHR10992:SF1002	ESTERASE PIR7A-RELATED	coord: 24..284

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0012012.1	Tan0012012.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009694	jasmonic acid metabolic process
biological_process	GO:0009696	salicylic acid metabolic process
molecular_function	GO:0080030	methyl indole-3-acetate esterase activity
molecular_function	GO:0080032	methyl jasmonate esterase activity
molecular_function	GO:0080031	methyl salicylate esterase activity