Tan0011300 (gene) Snake gourd v1

Overview
NameTan0011300
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionDelta 9 desaturase
LocationLG04: 84951004 .. 84953800 (-)
RNA-Seq ExpressionTan0011300
SyntenyTan0011300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGAAGCCTCTAAGGTCATTTGGGAAGAGAAAATGGGCCAAGATTGACAAAGACCTTGCTCGTTCTCTCTTCTTCATGCATTTGCTTTGTATTTTTGCACCATTTCACTTCAATTGGAGTGCATTTTGGGTTGCTTTTACATTATACTTTATCACCGGTCTCTTAGGCATTACTATTTCTTATCATAGAAATCTAGCACATCGAAGTTTTAATCTTCCCAAATGGCTTGAATACTTGTTTGCTTATTGTGGAGCTCATGCACTTCAGGTTCGTTCTTTTTACCATTAGAAATGCTATATTAATAAACATGTGCGTTGATTATAAAGGAACGGATAATAAATACACTTTAAAACATTTCAAATTTTCTCAATATATATATATTCATTGAAATTTGAAAATTTTCATTATTTTTTGAACTCCAGGGTGATCCAATTGATTGGGTGAGTACACACAGGTGTCACCATCGATTTGTTGATACTGAAAGAGACCCGCATAGCCCTATTCAAGGATTTTGGTTTAGTCACATTACTTGGCTTTTTGATTCGTATGCTTTGACCGAAAAAGTTTGTCCAAAAAATTCTAATGATTTTCGAAAGATAGAGAGAAACGTCTTTGTGTTGTTTACGAAGCAAGGAAGACCAGAAAATGTTGGGGACTTGGAGAAACAAACATTTTATAGATTTATTCACAGGACATACTTTCTTCATCCAATTATTCTTGCACTCATACTCTATGCTCTTGGTGGAGCTCCTTTTCTTATATGGGGAATGGTACAATAAAAGCACTCTCATATTTAATTTGTCTTGTTTCAATTATTAATCTATTTTTATTTATGTTAAGGTCATTTTTTAAAATAAGATATTAAGAAAAATGAAAATTTATTTGAGTAAGGTTTGAACTTCAACATCTCAAAGGTTCAACCATCCTCCCAAATAGTGGCCATGAATGGGAATAAAGTGCTAAGTTATTATTATTATTATTATTATTATTATTATTTATTTTTGAAGAAATGCTGAAGTGCATATATTAGAGTCTCCATATTCTTTGTCCCAAAACAAAAACTTATATATACATTATTTATTTTTATAAATTGAAAGGTTTTTAGAATGTCGAATCTTGTGAGAAGAATTCTCTGTTTATTTTTTCATTTGTTCTTATATTAAATAAGAATTATTTAAAAATGTGTAGTTTTATATTTAAGTTATATATTTTATGTCAAAATGAAAATATCATATATCAGTTAACGTATTTCTTTTTTAGTTGGATGATTAGAACTTCTAATCTTTTAATTGAAAATATAATCTCTTAATCAGTTGAACGATGTTAATACTGATAAGTTAAAGAATTTCTTATATAATATATAATCTAGTTTATAGTCATTAAAAATGTGTACAAAAGAAATGTGACATATTAAGTTATCTGTGCAGGGTGTAAGGATCATAGTGGTCTTACATATAACATTTATGGTGAATTCTATATGTCATATATGGGGAAAACGACAGTGGAGAACAAACGATTTATCAAGAAATAATTGGTAATTTTCTTTAATTTATAGTTTTTTTCCATTATCAAGAACACAAATGATTTAAATGATTGATTATACTAGACTTAGTTTGAGATAGTATAGCTTTTAAAATAATTATGGTAGTTGTGCTTTAGAAAAGTCAGTTGTGAAATAAAGTAAAATAATGTGTTCGGTAAGTCTCAATTTTAAATTAGAAAAAACTGATTAGGGTGTTTGATAAGTTAATACTAAATTATTGTGATTTAGTTATAATGAGTTAAGTATGCTTATTATTTCAATTATTTTTATATTGATATTTAATTTATAATTTATTATAAGATAATTGTTTAAAAATTTATATGATTTATTTCAACTAATTGTTTATCTAAATTATTTGATAAAAATTTAAGAAAATAATTGTTATACAAAATTGAATAAATCAGAGTTCCATTGATATAAGAGAAAATAGAGGTAAAAAATAAAAATAAAAATATAGAATGATAAAAATACTCAACCAAAAAAAGTAATTTCTATCCAGGTACAATTCTAGTCCCTAGTTTTTAGAAATAGATTTAAATTATTTTTTTTTATTAATTTTAATGTTGAATGAAATCTTATTAAGTTAAAAAAAAGAAGAAGTATTTTTTCTCCCTTCATTCTCTCCCCCTCATAAATAGTCTCTATTGAATAAGAACTATTCAGGAGAGTTTTATCAAATCATAGAGACTATTTTTAGATTTTATGAAAGTATAAGTACGAAATTCTAACTATTTAAATCATATAGACTAAATTTTAACATTTTCAAAACCAAACATACCAAATTTATATTTTAATCTTAATAATTATTAGTCAATTTCAATAGATTAATGTGTTATAACTTCAATTAAAAGGTCAAAATTTCAATTTTCGCCTCATAAATGGTCGAACTCAAATAATAATAATAAAAAATATTACGATTGTGATATAGTTTTAAATCCACCCTAATTTTTTTTTAGTTCAAAATACACCTCCTGTTTATAGAATTAATTATGGCATTGTATTTTACTATTTGTTATTAAATTTTACCTTCTTTATAGGTGGGTGGGTATACTTTCATTTGGAGAAGGTTGGCACAATAACCACCATGCCTTTGAATATTCAGCTAGACATGGGCTTGAATGGTGGCAAATTGACTTTGGTTGGTATGTTGTTTTGTTTCTTCAAGCCATTGGTGTCGCCACTAATGTCAAAGTACCCTCTCAAAGCCACAAGAAAAAGTTGGCATTTGTCAAATTACCTGAAGAAAATTAA

mRNA sequence

ATGGTGAAGCCTCTAAGGTCATTTGGGAAGAGAAAATGGGCCAAGATTGACAAAGACCTTGCTCGTTCTCTCTTCTTCATGCATTTGCTTTGTATTTTTGCACCATTTCACTTCAATTGGAGTGCATTTTGGGTTGCTTTTACATTATACTTTATCACCGGTCTCTTAGGCATTACTATTTCTTATCATAGAAATCTAGCACATCGAAGTTTTAATCTTCCCAAATGGCTTGAATACTTGTTTGCTTATTGTGGAGCTCATGCACTTCAGGGTGATCCAATTGATTGGGTGAGTACACACAGGTGTCACCATCGATTTGTTGATACTGAAAGAGACCCGCATAGCCCTATTCAAGGATTTTGGTTTAGTCACATTACTTGGCTTTTTGATTCGTATGCTTTGACCGAAAAAGTTTGTCCAAAAAATTCTAATGATTTTCGAAAGATAGAGAGAAACGTCTTTGTGTTGTTTACGAAGCAAGGAAGACCAGAAAATGTTGGGGACTTGGAGAAACAAACATTTTATAGATTTATTCACAGGACATACTTTCTTCATCCAATTATTCTTGCACTCATACTCTATGCTCTTGGTGGAGCTCCTTTTCTTATATGGGGAATGGGTGTAAGGATCATAGTGGTCTTACATATAACATTTATGGTGAATTCTATATGTCATATATGGGGAAAACGACAGTGGAGAACAAACGATTTATCAAGAAATAATTGGTGGGTGGGTATACTTTCATTTGGAGAAGGTTGGCACAATAACCACCATGCCTTTGAATATTCAGCTAGACATGGGCTTGAATGGTGGCAAATTGACTTTGGTTGGTATGTTGTTTTGTTTCTTCAAGCCATTGGTGTCGCCACTAATGTCAAAGTACCCTCTCAAAGCCACAAGAAAAAGTTGGCATTTGTCAAATTACCTGAAGAAAATTAA

Coding sequence (CDS)

ATGGTGAAGCCTCTAAGGTCATTTGGGAAGAGAAAATGGGCCAAGATTGACAAAGACCTTGCTCGTTCTCTCTTCTTCATGCATTTGCTTTGTATTTTTGCACCATTTCACTTCAATTGGAGTGCATTTTGGGTTGCTTTTACATTATACTTTATCACCGGTCTCTTAGGCATTACTATTTCTTATCATAGAAATCTAGCACATCGAAGTTTTAATCTTCCCAAATGGCTTGAATACTTGTTTGCTTATTGTGGAGCTCATGCACTTCAGGGTGATCCAATTGATTGGGTGAGTACACACAGGTGTCACCATCGATTTGTTGATACTGAAAGAGACCCGCATAGCCCTATTCAAGGATTTTGGTTTAGTCACATTACTTGGCTTTTTGATTCGTATGCTTTGACCGAAAAAGTTTGTCCAAAAAATTCTAATGATTTTCGAAAGATAGAGAGAAACGTCTTTGTGTTGTTTACGAAGCAAGGAAGACCAGAAAATGTTGGGGACTTGGAGAAACAAACATTTTATAGATTTATTCACAGGACATACTTTCTTCATCCAATTATTCTTGCACTCATACTCTATGCTCTTGGTGGAGCTCCTTTTCTTATATGGGGAATGGGTGTAAGGATCATAGTGGTCTTACATATAACATTTATGGTGAATTCTATATGTCATATATGGGGAAAACGACAGTGGAGAACAAACGATTTATCAAGAAATAATTGGTGGGTGGGTATACTTTCATTTGGAGAAGGTTGGCACAATAACCACCATGCCTTTGAATATTCAGCTAGACATGGGCTTGAATGGTGGCAAATTGACTTTGGTTGGTATGTTGTTTTGTTTCTTCAAGCCATTGGTGTCGCCACTAATGTCAAAGTACCCTCTCAAAGCCACAAGAAAAAGTTGGCATTTGTCAAATTACCTGAAGAAAATTAA

Protein sequence

MVKPLRSFGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITISYHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGFWFSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHRTYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRNNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHKKKLAFVKLPEEN
Homology
BLAST of Tan0011300 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 414.5 bits (1064), Expect = 1.1e-114
Identity = 181/296 (61.15%), Postives = 226/296 (76.35%), Query Frame = 0

Query: 10  KRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITISYHRNLAHR 69
           +R+W  +D      +  MHLL + APF FNW A  VAF LY +TGLLGIT+S+HRNL+H+
Sbjct: 95  EREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHK 154

Query: 70  SFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGFWFSHITWLF 129
           +F LPKWLEYLFAYCGA ALQG+PIDWVSTHR HH+F D++RDPHSP+ GFWFSH+ W+F
Sbjct: 155 AFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMF 214

Query: 130 DSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHRTYFLHPIIL 189
           D+  +T++                       G P NVGDLEKQ FYRF+  TY LHP+ L
Sbjct: 215 DTNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLAL 274

Query: 190 ALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRNNWWVGILSF 249
           A+ LYA+GG PF++WGMGVRI+ V HIT++VNS CH+WGK+ W T DLS+NNWWV  L+F
Sbjct: 275 AVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAF 334

Query: 250 GEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHKKKLAF 306
           GEGWHNNHHAFE+SARHGLEWWQ+D  WYVV FLQAIG+AT+VK+PS++ K+++AF
Sbjct: 335 GEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMAF 368

BLAST of Tan0011300 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.4e-100
Identity = 163/307 (53.09%), Postives = 212/307 (69.06%), Query Frame = 0

Query: 1   MVKPLRSFGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITI 60
           M +  R+  +RKW ++D   A +  F+H LC+ APF+F W A  VA  +Y + G LGIT+
Sbjct: 22  MGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGG-LGITV 81

Query: 61  SYHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGF 120
           SYHRNLAHRSF +PKWLEY FAYCG  A+QGDPIDWVSTHR HH+F D++RDPHSP +GF
Sbjct: 82  SYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGF 141

Query: 121 WFSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHR 180
           WFSH+ WLFD+  L EK                       GR  NV DL++Q +Y+F+ R
Sbjct: 142 WFSHLLWLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQR 201

Query: 181 TYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRN 240
           T   H +    +LY  GG  FL WGMG+ + +  H+T ++NS+CH+WG R W+TND SRN
Sbjct: 202 TVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRN 261

Query: 241 NWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHK 300
            WW+ + SFGE WHNNHHAFE SAR GLEWWQID  WY+V FL+ IG+AT+VK+PS+S +
Sbjct: 262 VWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQR 305

Query: 301 KKLAFVK 308
           +++A V+
Sbjct: 322 RRMAMVR 305

BLAST of Tan0011300 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 4.5e-100
Identity = 161/295 (54.58%), Postives = 206/295 (69.83%), Query Frame = 0

Query: 11  RKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITISYHRNLAHRS 70
           RKW   D      +  +H+LC+FAP  F+W +FWV F LY I G+ G T+S+HRNL HRS
Sbjct: 38  RKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRS 97

Query: 71  FNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGFWFSHITWLFD 130
           F LPK+LEY FAY G HALQGDP+ WVSTHR HH++ DT  DPHSPI+GFWF HI WLFD
Sbjct: 98  FKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFD 157

Query: 131 SYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHRTYFLHPIILA 190
           S  + EK                       GR EN GDL KQ++YRF+ RT+  H  + A
Sbjct: 158 SKYIIEKC----------------------GRYENAGDLMKQSYYRFLERTFVYHVYLQA 217

Query: 191 LILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRNNWWVGILSFG 250
            +LY  GG PF++WGM VR I+  H++++VNS+CH WG R W T DLS NNW++ +L+ G
Sbjct: 218 ALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSG 277

Query: 251 EGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHKKKLAF 306
           EGWHNNHHAFEYSARHG+EWWQID  WY++  L+ +G+AT++KVPS+ HK+K++F
Sbjct: 278 EGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPSEIHKRKMSF 310

BLAST of Tan0011300 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 361.3 bits (926), Expect = 1.1e-98
Identity = 165/306 (53.92%), Postives = 208/306 (67.97%), Query Frame = 0

Query: 1   MVKPLRSFGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITI 60
           +V+  RSF +R W   D          HLL + APF+F+W AFWV   L FI G+  IT+
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITL 138

Query: 61  SYHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGF 120
           SYHRNL+HRSF+LPKWLEYLFAY G  A QGDPI+WVS HR HH+  +T+RDPHSP QGF
Sbjct: 139 SYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGF 198

Query: 121 WFSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHR 180
           WFSH+ W+FD+ ++ E                        G  ENV DL +Q FYRF+ R
Sbjct: 199 WFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFLQR 258

Query: 181 TYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRN 240
           T  LH +  + + Y  GG P L+WG+G+ I V LH+TF+VNS+CHIWG R W T+D S+N
Sbjct: 259 TVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKN 318

Query: 241 NWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHK 300
           NWWV IL+ GEGWHNNHHAFE+SARHGLEWWQ+D  W ++ FL+AIG+ATNVK+P+++  
Sbjct: 319 NWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQM 361

Query: 301 KKLAFV 307
           K  A V
Sbjct: 379 KGKALV 361

BLAST of Tan0011300 vs. ExPASy Swiss-Prot
Match: Q9SID2 (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=2)

HSP 1 Score: 359.4 bits (921), Expect = 4.2e-98
Identity = 161/300 (53.67%), Postives = 205/300 (68.33%), Query Frame = 0

Query: 8   FGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITISYHRNLA 67
           F  R+W ++D     + F +H L + APF+F WSA WV F  Y I G LGIT+SYHRNLA
Sbjct: 31  FWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGG-LGITVSYHRNLA 90

Query: 68  HRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGFWFSHITW 127
           HRSF +PKWLEYL AYC   A+QGDPIDWVSTHR HH+F D+ERDPHSP +GFWFSH+ W
Sbjct: 91  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 150

Query: 128 LFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHRTYFLHPI 187
           ++DS  L  K                       GR  NV DL++Q FYRF+ +T   H +
Sbjct: 151 IYDSAYLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHIL 210

Query: 188 ILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRNNWWVGIL 247
            L   L+ LGG  F+ WGMGV   + +H+T ++NS+CHIWG R W+TND SRN WW+ + 
Sbjct: 211 GLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVF 270

Query: 248 SFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHKKKLAFVK 307
           SFGE WHNNHHAFE SAR GLEWWQID  WY+V F + IG+AT+VKVP+++ ++++A V+
Sbjct: 271 SFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQRRRMAIVR 307

BLAST of Tan0011300 vs. NCBI nr
Match: XP_022153632.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia] >XP_022153633.1 palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 563.1 bits (1450), Expect = 1.5e-156
Identity = 249/312 (79.81%), Postives = 285/312 (91.35%), Query Frame = 0

Query: 1   MVKPLRSFGKR-KWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGIT 60
           M  P +SFGKR KW K+DKD+A SLFF+HLL +FAPF+FNW AFW+AF LY  TGL GI+
Sbjct: 31  MAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFAPFYFNWRAFWIAFALYVATGLFGIS 90

Query: 61  ISYHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQG 120
           +SYHRNLAH+SF LPKWLEYLFAYCGAHALQGDPIDWV+THRCHHRFVDTERDPHSPIQG
Sbjct: 91  VSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPIDWVNTHRCHHRFVDTERDPHSPIQG 150

Query: 121 FWFSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIH 180
           FWFSHITWL DSY+LT++VCPK   DF+K+ERN+FV+F+K GRP+NVGDLEKQTFYRFIH
Sbjct: 151 FWFSHITWLLDSYSLTKRVCPKYFTDFQKVERNIFVMFSKHGRPDNVGDLEKQTFYRFIH 210

Query: 181 RTYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSR 240
           +TYFLHPIILA+ILYA GGAPFLIWGMGVRI+VVLHIT M+NS+CHIWGK+ WRT DLSR
Sbjct: 211 KTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVLHITCMINSVCHIWGKQPWRTGDLSR 270

Query: 241 NNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSH 300
           NNWWV ++SFGEGWHNNHHAFEYSARHG+EWWQIDFGWYVV+FL+AIG+ATNVKVPSQ+ 
Sbjct: 271 NNWWVSLVSFGEGWHNNHHAFEYSARHGIEWWQIDFGWYVVMFLEAIGIATNVKVPSQNQ 330

Query: 301 KKKLAFVKLPEE 312
           K+KLA +  P+E
Sbjct: 331 KQKLA-IDAPKE 341

BLAST of Tan0011300 vs. NCBI nr
Match: XP_011652978.2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus] >KAE8649060.1 hypothetical protein Csa_015380 [Cucumis sativus])

HSP 1 Score: 540.4 bits (1391), Expect = 1.0e-149
Identity = 243/304 (79.93%), Postives = 272/304 (89.47%), Query Frame = 0

Query: 2   VKPLRSFGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITIS 61
           VKPLRSF K+KWAKI++ +A SL F+HLLCIFAPFHFNWSAFWVAF LY ITGL GI++S
Sbjct: 13  VKPLRSFRKKKWAKINRSVAGSLLFVHLLCIFAPFHFNWSAFWVAFVLYVITGLFGISVS 72

Query: 62  YHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGFW 121
           YHRNLAHRSFNLPKWLEYLFAYCG HALQGDPI WV  HRCHHRFVDTE+DPHSPIQGF 
Sbjct: 73  YHRNLAHRSFNLPKWLEYLFAYCGVHALQGDPIGWVKIHRCHHRFVDTEKDPHSPIQGFL 132

Query: 122 FSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPE-NVGDLEKQTFYRFIHR 181
           FSH+TWL DSY LTEKV PK  +D+ KIERNV ++  KQGRPE NVGDLEKQ FYRF+ +
Sbjct: 133 FSHVTWLLDSYVLTEKVGPKYFDDYEKIERNVVMVNMKQGRPENNVGDLEKQAFYRFLRK 192

Query: 182 TYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRN 241
           TYFLH ++LA++LYA+GG PFLIWGMGVRI+VVLHITFMVNS+CHIWGK+ W+TNDLS N
Sbjct: 193 TYFLHLLLLAVLLYAMGGVPFLIWGMGVRIVVVLHITFMVNSVCHIWGKQLWKTNDLSTN 252

Query: 242 NWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHK 301
           NW VG+L FGEGWHNNHHAFEYSARHGLEWW+IDFGWYV++FLQAIGVAT+VKVP Q HK
Sbjct: 253 NWLVGLLGFGEGWHNNHHAFEYSARHGLEWWEIDFGWYVIMFLQAIGVATHVKVPLQHHK 312

Query: 302 KKLA 305
           KKLA
Sbjct: 313 KKLA 316

BLAST of Tan0011300 vs. NCBI nr
Match: XP_022153644.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 532.7 bits (1371), Expect = 2.1e-147
Identity = 227/299 (75.92%), Postives = 267/299 (89.30%), Query Frame = 0

Query: 1   MVKPLRSFGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITI 60
           M+KP R + +RKW K+DKD+A SLF +HLLCI APF FNWSAFWVAF LY +TGL G+T+
Sbjct: 11  MMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLYIVTGLFGMTL 70

Query: 61  SYHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGF 120
           SYHRNLAH+SF LPKWLEYLFAYCG  ALQGDP+DWVSTHRCHH+FVDTERDPHSPI+GF
Sbjct: 71  SYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTERDPHSPIRGF 130

Query: 121 WFSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHR 180
           WFSHI WLFDSY LT+KVCPK  ++F+K+ERN+FVLF+K G+PENV DLEKQ FYRFIH+
Sbjct: 131 WFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLEKQAFYRFIHQ 190

Query: 181 TYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRN 240
           TY LHPI+LA+ILY +GG PFL+WGM VRI+VVLH T MVNS+CHIWGK+ W T D+S+N
Sbjct: 191 TYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGKQSWTTGDMSKN 250

Query: 241 NWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSH 300
           NWWV +++FGEGWHNNHHAFEYSA+ GLEWWQ+DFGWY++LFLQAIGVAT+VK+PSQSH
Sbjct: 251 NWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVATHVKLPSQSH 309

BLAST of Tan0011300 vs. NCBI nr
Match: XP_022979557.1 (delta-9 acyl-lipid desaturase 1-like [Cucurbita maxima])

HSP 1 Score: 512.7 bits (1319), Expect = 2.2e-141
Identity = 228/312 (73.08%), Postives = 265/312 (84.94%), Query Frame = 0

Query: 1   MVKPLRSFGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITI 60
           MVKP RSF +RKW K+DK +A  +  +HLLCIFAPFHF W AF VA TL  ITGL G+TI
Sbjct: 28  MVKPPRSFWRRKWTKLDKRVAHVILLLHLLCIFAPFHFTWPAFRVAVTLQIITGLFGMTI 87

Query: 61  SYHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGF 120
           SYHRNLAH+SF +PKWLEYLFAYCG HALQGDPIDWVSTHRCHH+FVDTERDPHSPIQGF
Sbjct: 88  SYHRNLAHKSFQIPKWLEYLFAYCGVHALQGDPIDWVSTHRCHHQFVDTERDPHSPIQGF 147

Query: 121 WFSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHR 180
           W SHI WLFDSY LT+KV PK S++ +KIE+N+FV F+K  +PENVGDLE+QTFYRFIH+
Sbjct: 148 WMSHIMWLFDSYTLTDKVSPKYSSNLQKIEKNMFVYFSKYRKPENVGDLERQTFYRFIHK 207

Query: 181 TYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRN 240
           TY LHPI LA ILY +GG PFLIWGM VRI+V+ H TFMVNS+CH+WG++ WRT DLSRN
Sbjct: 208 TYLLHPIALAAILYTVGGVPFLIWGMCVRIVVIFHSTFMVNSVCHMWGQQHWRTGDLSRN 267

Query: 241 NWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHK 300
           NWWVG+ SFGEGWHNNHHAF+YSAR GLEWWQ+D GWYV+ FLQ IG+AT+VK+PS +H 
Sbjct: 268 NWWVGLTSFGEGWHNNHHAFKYSARLGLEWWQLDAGWYVIRFLQVIGLATDVKLPSPAHM 327

Query: 301 KKLAFVKLPEEN 313
           + LA  K PE++
Sbjct: 328 QALALNK-PEDH 338

BLAST of Tan0011300 vs. NCBI nr
Match: KAG6581933.1 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 509.2 bits (1310), Expect = 2.5e-140
Identity = 226/312 (72.44%), Postives = 264/312 (84.62%), Query Frame = 0

Query: 1   MVKPLRSFGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITI 60
           MVKP RSF +RKW K+DK +A  +  +HLLCI APFHF W AF VA TL  ITGL G+TI
Sbjct: 29  MVKPPRSFWRRKWTKLDKRVAHVILLLHLLCILAPFHFTWPAFRVAVTLQIITGLFGMTI 88

Query: 61  SYHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGF 120
           SYHRNLAH+SF +PKWLEYLFAYCG HALQGDPIDWVSTHRCHH+FVDTERDPHSPIQGF
Sbjct: 89  SYHRNLAHKSFQIPKWLEYLFAYCGVHALQGDPIDWVSTHRCHHQFVDTERDPHSPIQGF 148

Query: 121 WFSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHR 180
           W SHI WLFDSY LT+KV PK S+D +KIE+N+FV F+K  +PENVGDLE+QTFYRFIH+
Sbjct: 149 WMSHIMWLFDSYTLTDKVSPKYSSDLQKIEKNMFVYFSKYRKPENVGDLERQTFYRFIHK 208

Query: 181 TYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRN 240
           TY LHP+ LA ILY +GG PFLIWGM VRI+V+ H TFMVNS+CH+WG++ WRT+DLSRN
Sbjct: 209 TYLLHPMALAAILYTVGGVPFLIWGMCVRIVVIFHSTFMVNSVCHMWGQQHWRTDDLSRN 268

Query: 241 NWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHK 300
           NWWVG+ SFGEGWHNNHHAF+YSAR GLEWWQ+D GWYV+  LQ IG+AT+VK+PS +H 
Sbjct: 269 NWWVGLTSFGEGWHNNHHAFKYSARLGLEWWQLDAGWYVIRILQVIGLATDVKLPSPAHM 328

Query: 301 KKLAFVKLPEEN 313
           + LA  K PE++
Sbjct: 329 QALALNK-PEDH 339

BLAST of Tan0011300 vs. ExPASy TrEMBL
Match: A0A6J1DHZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021091 PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 7.0e-157
Identity = 249/312 (79.81%), Postives = 285/312 (91.35%), Query Frame = 0

Query: 1   MVKPLRSFGKR-KWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGIT 60
           M  P +SFGKR KW K+DKD+A SLFF+HLL +FAPF+FNW AFW+AF LY  TGL GI+
Sbjct: 31  MAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFAPFYFNWRAFWIAFALYVATGLFGIS 90

Query: 61  ISYHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQG 120
           +SYHRNLAH+SF LPKWLEYLFAYCGAHALQGDPIDWV+THRCHHRFVDTERDPHSPIQG
Sbjct: 91  VSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPIDWVNTHRCHHRFVDTERDPHSPIQG 150

Query: 121 FWFSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIH 180
           FWFSHITWL DSY+LT++VCPK   DF+K+ERN+FV+F+K GRP+NVGDLEKQTFYRFIH
Sbjct: 151 FWFSHITWLLDSYSLTKRVCPKYFTDFQKVERNIFVMFSKHGRPDNVGDLEKQTFYRFIH 210

Query: 181 RTYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSR 240
           +TYFLHPIILA+ILYA GGAPFLIWGMGVRI+VVLHIT M+NS+CHIWGK+ WRT DLSR
Sbjct: 211 KTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVLHITCMINSVCHIWGKQPWRTGDLSR 270

Query: 241 NNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSH 300
           NNWWV ++SFGEGWHNNHHAFEYSARHG+EWWQIDFGWYVV+FL+AIG+ATNVKVPSQ+ 
Sbjct: 271 NNWWVSLVSFGEGWHNNHHAFEYSARHGIEWWQIDFGWYVVMFLEAIGIATNVKVPSQNQ 330

Query: 301 KKKLAFVKLPEE 312
           K+KLA +  P+E
Sbjct: 331 KQKLA-IDAPKE 341

BLAST of Tan0011300 vs. ExPASy TrEMBL
Match: A0A6J1DJP0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021101 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 1.0e-147
Identity = 227/299 (75.92%), Postives = 267/299 (89.30%), Query Frame = 0

Query: 1   MVKPLRSFGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITI 60
           M+KP R + +RKW K+DKD+A SLF +HLLCI APF FNWSAFWVAF LY +TGL G+T+
Sbjct: 11  MMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLYIVTGLFGMTL 70

Query: 61  SYHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGF 120
           SYHRNLAH+SF LPKWLEYLFAYCG  ALQGDP+DWVSTHRCHH+FVDTERDPHSPI+GF
Sbjct: 71  SYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTERDPHSPIRGF 130

Query: 121 WFSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHR 180
           WFSHI WLFDSY LT+KVCPK  ++F+K+ERN+FVLF+K G+PENV DLEKQ FYRFIH+
Sbjct: 131 WFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLEKQAFYRFIHQ 190

Query: 181 TYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRN 240
           TY LHPI+LA+ILY +GG PFL+WGM VRI+VVLH T MVNS+CHIWGK+ W T D+S+N
Sbjct: 191 TYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGKQSWTTGDMSKN 250

Query: 241 NWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSH 300
           NWWV +++FGEGWHNNHHAFEYSA+ GLEWWQ+DFGWY++LFLQAIGVAT+VK+PSQSH
Sbjct: 251 NWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVATHVKLPSQSH 309

BLAST of Tan0011300 vs. ExPASy TrEMBL
Match: A0A6J1IP09 (delta-9 acyl-lipid desaturase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111479233 PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.1e-141
Identity = 228/312 (73.08%), Postives = 265/312 (84.94%), Query Frame = 0

Query: 1   MVKPLRSFGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITI 60
           MVKP RSF +RKW K+DK +A  +  +HLLCIFAPFHF W AF VA TL  ITGL G+TI
Sbjct: 28  MVKPPRSFWRRKWTKLDKRVAHVILLLHLLCIFAPFHFTWPAFRVAVTLQIITGLFGMTI 87

Query: 61  SYHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGF 120
           SYHRNLAH+SF +PKWLEYLFAYCG HALQGDPIDWVSTHRCHH+FVDTERDPHSPIQGF
Sbjct: 88  SYHRNLAHKSFQIPKWLEYLFAYCGVHALQGDPIDWVSTHRCHHQFVDTERDPHSPIQGF 147

Query: 121 WFSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHR 180
           W SHI WLFDSY LT+KV PK S++ +KIE+N+FV F+K  +PENVGDLE+QTFYRFIH+
Sbjct: 148 WMSHIMWLFDSYTLTDKVSPKYSSNLQKIEKNMFVYFSKYRKPENVGDLERQTFYRFIHK 207

Query: 181 TYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRN 240
           TY LHPI LA ILY +GG PFLIWGM VRI+V+ H TFMVNS+CH+WG++ WRT DLSRN
Sbjct: 208 TYLLHPIALAAILYTVGGVPFLIWGMCVRIVVIFHSTFMVNSVCHMWGQQHWRTGDLSRN 267

Query: 241 NWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHK 300
           NWWVG+ SFGEGWHNNHHAF+YSAR GLEWWQ+D GWYV+ FLQ IG+AT+VK+PS +H 
Sbjct: 268 NWWVGLTSFGEGWHNNHHAFKYSARLGLEWWQLDAGWYVIRFLQVIGLATDVKLPSPAHM 327

Query: 301 KKLAFVKLPEEN 313
           + LA  K PE++
Sbjct: 328 QALALNK-PEDH 338

BLAST of Tan0011300 vs. ExPASy TrEMBL
Match: A0A1S4E0J1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494694 PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 2.1e-140
Identity = 230/312 (73.72%), Postives = 261/312 (83.65%), Query Frame = 0

Query: 1   MVK-PLRSFGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGIT 60
           MVK P+RSF +RKW  +DK +A  + F+HLLCIFAPF FNWSAF VA  L  ITGL G+T
Sbjct: 33  MVKPPVRSFWRRKWTTLDKRVAYIISFVHLLCIFAPFQFNWSAFRVAVALKVITGLFGMT 92

Query: 61  ISYHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQG 120
           ISYHRNLAH+SF LPKWLEYLFAYCG HALQGDPIDWVSTHRCHH+FVDTE+DPHSPI G
Sbjct: 93  ISYHRNLAHKSFQLPKWLEYLFAYCGVHALQGDPIDWVSTHRCHHQFVDTEKDPHSPING 152

Query: 121 FWFSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIH 180
           FW SH+ WLFDSY LT KVCPK S+D +KIERN+FV+FTK G+P+NVGDLEKQ FYRFIH
Sbjct: 153 FWISHMMWLFDSYTLTNKVCPKYSSDLKKIERNMFVIFTKHGKPDNVGDLEKQRFYRFIH 212

Query: 181 RTYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSR 240
           +TY LH + LA+ILY +GG PF IWGM VRI+VV H TFMVNS+CHIWGK+ WRT DLSR
Sbjct: 213 KTYMLHHLTLAIILYNVGGLPFFIWGMCVRIVVVFHSTFMVNSVCHIWGKQPWRTGDLSR 272

Query: 241 NNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSH 300
           NNW VG+ SFGEGWHNNHHAF YSAR GLEWWQ+D GWYV+ FLQ IG+ATNVK+PS SH
Sbjct: 273 NNWLVGLTSFGEGWHNNHHAFSYSARLGLEWWQLDAGWYVIKFLQVIGLATNVKLPSPSH 332

Query: 301 KKKLAFVKLPEE 312
            + LA    P+E
Sbjct: 333 MQNLAVDHKPKE 344

BLAST of Tan0011300 vs. ExPASy TrEMBL
Match: A0A1S3BZB9 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494694 PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 2.1e-140
Identity = 230/312 (73.72%), Postives = 261/312 (83.65%), Query Frame = 0

Query: 1   MVK-PLRSFGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGIT 60
           MVK P+RSF +RKW  +DK +A  + F+HLLCIFAPF FNWSAF VA  L  ITGL G+T
Sbjct: 34  MVKPPVRSFWRRKWTTLDKRVAYIISFVHLLCIFAPFQFNWSAFRVAVALKVITGLFGMT 93

Query: 61  ISYHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQG 120
           ISYHRNLAH+SF LPKWLEYLFAYCG HALQGDPIDWVSTHRCHH+FVDTE+DPHSPI G
Sbjct: 94  ISYHRNLAHKSFQLPKWLEYLFAYCGVHALQGDPIDWVSTHRCHHQFVDTEKDPHSPING 153

Query: 121 FWFSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIH 180
           FW SH+ WLFDSY LT KVCPK S+D +KIERN+FV+FTK G+P+NVGDLEKQ FYRFIH
Sbjct: 154 FWISHMMWLFDSYTLTNKVCPKYSSDLKKIERNMFVIFTKHGKPDNVGDLEKQRFYRFIH 213

Query: 181 RTYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSR 240
           +TY LH + LA+ILY +GG PF IWGM VRI+VV H TFMVNS+CHIWGK+ WRT DLSR
Sbjct: 214 KTYMLHHLTLAIILYNVGGLPFFIWGMCVRIVVVFHSTFMVNSVCHIWGKQPWRTGDLSR 273

Query: 241 NNWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSH 300
           NNW VG+ SFGEGWHNNHHAF YSAR GLEWWQ+D GWYV+ FLQ IG+ATNVK+PS SH
Sbjct: 274 NNWLVGLTSFGEGWHNNHHAFSYSARLGLEWWQLDAGWYVIKFLQVIGLATNVKLPSPSH 333

Query: 301 KKKLAFVKLPEE 312
            + LA    P+E
Sbjct: 334 MQNLAVDHKPKE 345

BLAST of Tan0011300 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 414.5 bits (1064), Expect = 7.8e-116
Identity = 181/296 (61.15%), Postives = 226/296 (76.35%), Query Frame = 0

Query: 10  KRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITISYHRNLAHR 69
           +R+W  +D      +  MHLL + APF FNW A  VAF LY +TGLLGIT+S+HRNL+H+
Sbjct: 95  EREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHK 154

Query: 70  SFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGFWFSHITWLF 129
           +F LPKWLEYLFAYCGA ALQG+PIDWVSTHR HH+F D++RDPHSP+ GFWFSH+ W+F
Sbjct: 155 AFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMF 214

Query: 130 DSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHRTYFLHPIIL 189
           D+  +T++                       G P NVGDLEKQ FYRF+  TY LHP+ L
Sbjct: 215 DTNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLAL 274

Query: 190 ALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRNNWWVGILSF 249
           A+ LYA+GG PF++WGMGVRI+ V HIT++VNS CH+WGK+ W T DLS+NNWWV  L+F
Sbjct: 275 AVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAF 334

Query: 250 GEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHKKKLAF 306
           GEGWHNNHHAFE+SARHGLEWWQ+D  WYVV FLQAIG+AT+VK+PS++ K+++AF
Sbjct: 335 GEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMAF 368

BLAST of Tan0011300 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 366.3 bits (939), Expect = 2.4e-101
Identity = 163/307 (53.09%), Postives = 212/307 (69.06%), Query Frame = 0

Query: 1   MVKPLRSFGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITI 60
           M +  R+  +RKW ++D   A +  F+H LC+ APF+F W A  VA  +Y + G LGIT+
Sbjct: 22  MGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGG-LGITV 81

Query: 61  SYHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGF 120
           SYHRNLAHRSF +PKWLEY FAYCG  A+QGDPIDWVSTHR HH+F D++RDPHSP +GF
Sbjct: 82  SYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGF 141

Query: 121 WFSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHR 180
           WFSH+ WLFD+  L EK                       GR  NV DL++Q +Y+F+ R
Sbjct: 142 WFSHLLWLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQR 201

Query: 181 TYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRN 240
           T   H +    +LY  GG  FL WGMG+ + +  H+T ++NS+CH+WG R W+TND SRN
Sbjct: 202 TVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRN 261

Query: 241 NWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHK 300
            WW+ + SFGE WHNNHHAFE SAR GLEWWQID  WY+V FL+ IG+AT+VK+PS+S +
Sbjct: 262 VWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQR 305

Query: 301 KKLAFVK 308
           +++A V+
Sbjct: 322 RRMAMVR 305

BLAST of Tan0011300 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 361.3 bits (926), Expect = 7.8e-100
Identity = 165/306 (53.92%), Postives = 208/306 (67.97%), Query Frame = 0

Query: 1   MVKPLRSFGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITI 60
           +V+  RSF +R W   D          HLL + APF+F+W AFWV   L FI G+  IT+
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITL 138

Query: 61  SYHRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGF 120
           SYHRNL+HRSF+LPKWLEYLFAY G  A QGDPI+WVS HR HH+  +T+RDPHSP QGF
Sbjct: 139 SYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGF 198

Query: 121 WFSHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHR 180
           WFSH+ W+FD+ ++ E                        G  ENV DL +Q FYRF+ R
Sbjct: 199 WFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFLQR 258

Query: 181 TYFLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRN 240
           T  LH +  + + Y  GG P L+WG+G+ I V LH+TF+VNS+CHIWG R W T+D S+N
Sbjct: 259 TVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKN 318

Query: 241 NWWVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHK 300
           NWWV IL+ GEGWHNNHHAFE+SARHGLEWWQ+D  W ++ FL+AIG+ATNVK+P+++  
Sbjct: 319 NWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQM 361

Query: 301 KKLAFV 307
           K  A V
Sbjct: 379 KGKALV 361

BLAST of Tan0011300 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 359.4 bits (921), Expect = 3.0e-99
Identity = 161/300 (53.67%), Postives = 205/300 (68.33%), Query Frame = 0

Query: 8   FGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITISYHRNLA 67
           F  R+W ++D     + F +H L + APF+F WSA WV F  Y I G LGIT+SYHRNLA
Sbjct: 31  FWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGG-LGITVSYHRNLA 90

Query: 68  HRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGFWFSHITW 127
           HRSF +PKWLEYL AYC   A+QGDPIDWVSTHR HH+F D+ERDPHSP +GFWFSH+ W
Sbjct: 91  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 150

Query: 128 LFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHRTYFLHPI 187
           ++DS  L  K                       GR  NV DL++Q FYRF+ +T   H +
Sbjct: 151 IYDSAYLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHIL 210

Query: 188 ILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRNNWWVGIL 247
            L   L+ LGG  F+ WGMGV   + +H+T ++NS+CHIWG R W+TND SRN WW+ + 
Sbjct: 211 GLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVF 270

Query: 248 SFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHKKKLAFVK 307
           SFGE WHNNHHAFE SAR GLEWWQID  WY+V F + IG+AT+VKVP+++ ++++A V+
Sbjct: 271 SFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQRRRMAIVR 307

BLAST of Tan0011300 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 329.7 bits (844), Expect = 2.5e-90
Identity = 152/302 (50.33%), Postives = 200/302 (66.23%), Query Frame = 0

Query: 3   KPLRSFGKRKWAKIDKDLARSLFFMHLLCIFAPFHFNWSAFWVAFTLYFITGLLGITISY 62
           K  R++  RKW + D   A ++  +HLLC+ APF++ W AF     L  +T L  IT SY
Sbjct: 18  KEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRFGIILAILTNLC-ITFSY 77

Query: 63  HRNLAHRSFNLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIQGFWF 122
           HRNL HRSF LPKWLEY FAY    ALQGDP+DWVS HR HH+F D++RDPHSPI+GFWF
Sbjct: 78  HRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRDPHSPIEGFWF 137

Query: 123 SHITWLFDSYALTEKVCPKNSNDFRKIERNVFVLFTKQGRPENVGDLEKQTFYRFIHRTY 182
           SH+ W+FD+  + EK                       GR  NV DL++Q FYRF+ +T 
Sbjct: 138 SHVLWIFDTDYIREKC----------------------GRRNNVMDLKQQWFYRFLKKTL 197

Query: 183 FLHPIILALILYALGGAPFLIWGMGVRIIVVLHITFMVNSICHIWGKRQWRTNDLSRNNW 242
            LH +    ++Y  GG P+L W +G   ++  H T++VNS CHI G + W+TND SRN W
Sbjct: 198 VLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHICGSQAWQTNDTSRNVW 257

Query: 243 WVGILSFGEGWHNNHHAFEYSARHGLEWWQIDFGWYVVLFLQAIGVATNVKVPSQSHKKK 302
           W+ +L+ GE WHNNHHAFE SARHGLEW+Q+D  WY++ F QA+G+ATNVK+P+ + K+K
Sbjct: 258 WLALLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQALGLATNVKLPTDAQKRK 296

Query: 303 LA 305
           +A
Sbjct: 318 MA 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949X01.1e-11461.15Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
O657973.4e-10053.09Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
P0DOW24.5e-10054.58Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
Q9LVZ31.1e-9853.92Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q9SID24.2e-9853.67Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
XP_022153632.11.5e-15679.81palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
XP_011652978.21.0e-14979.93palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi... [more]
XP_022153644.12.1e-14775.92palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
XP_022979557.12.2e-14173.08delta-9 acyl-lipid desaturase 1-like [Cucurbita maxima][more]
KAG6581933.12.5e-14072.44Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partia... [more]
Match NameE-valueIdentityDescription
A0A6J1DHZ77.0e-15779.81palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DJP01.0e-14775.92palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1IP091.1e-14173.08delta-9 acyl-lipid desaturase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111479233... [more]
A0A1S4E0J12.1e-14073.72palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like is... [more]
A0A1S3BZB92.1e-14073.72palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like is... [more]
Match NameE-valueIdentityDescription
AT3G15850.17.8e-11661.15fatty acid desaturase 5 [more]
AT1G06080.12.4e-10153.09delta 9 desaturase 1 [more]
AT3G15870.17.8e-10053.92Fatty acid desaturase family protein [more]
AT2G31360.13.0e-9953.6716:0delta9 desaturase 2 [more]
AT1G06100.12.5e-9050.33Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 40..62
score: 24.03
coord: 100..129
score: 52.28
coord: 63..83
score: 30.08
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 10..158
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 158..307
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 40..293
e-value: 2.71766E-71
score: 216.653
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 43..275
e-value: 1.5E-20
score: 74.1
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 158..307
coord: 10..158

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0011300.1Tan0011300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0009979 16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water