Tan0011229 (gene) Snake gourd v1

Overview
NameTan0011229
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptioncharged multivesicular body protein 7
LocationLG11: 16040829 .. 16043500 (-)
RNA-Seq ExpressionTan0011229
SyntenyTan0011229
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAAGGAATCAAAGGGATCGTGTGTTAGAGAGTTCATCAGGGAAAAAGTCCCTGACTGGGATGACGAAGTGGTGGCTACAGCTCGGTTCAAGGCATTTAGTGGGCAAAAATCTGATTGGGAACCCAGATACCTATTTTGGAGGGATTTGATCCTCACAATTGCTCATCAATTCAACTTCATCTTCATTAAATCTTCTGAAATAAGGACTCAGTGGTTTTCTCGAGGAGGGTTGGCTCCACTGTGCCTTGACCATGTTCTGGTATTCGTTGACTAAGATGATTGTTTGCTATCTCTAATTTATTGAACTTTGTTGTTCTGTCCTGTAATAGTTCACCATCCCTTTCAGCATCTAATGTATATTGAGGGCGACATTATAAGACGTAGTGACATGTTGGACCCGAGGAGTGGCCAACTCTCTTACTTGTTTAAAAAACTAAGCAATTTGATGGGTACATCCAAAAAGAACGCCGATGATTTGATTCGCGATGATTATATAGTTCTTGCCTCTGTATTAAAGGTATATGCTTTAAGTCTCCTCTCTCTCTTGCTCAAATCCTTTTTAGATTCACTGCTTTTCCTTTTCTTTAGAGACATGACTTATTAAGATCTGCTTTGAAATTTACTTCCAGGTTCATCTTAATTTTTCATTACTTTTGGTTTATGCTTCTATAACTTTAATATGAAATGTTTCCATGCCAAGGATAGAGCAACTGAAGTCGTCAAGTGTTTATCTCAAAGTAATTGGACCTCTTCCTGCATTGTTACAATGGTGAAGTTCCAGAACCTCTGTGGAGGACCTGATGAAGCGACTGCCATCTTGAGTTACTTAATTGGATGTGGTAAAGCAAGGTATCTCTCTAGGGAAAAAAAGGATCTCTTAGAGGTATGACCATTGTTTCCTTGTTTCATATTTCATCCTTCATATGAATACATTCTTATTTTCTTTTTATAATTTATAAATATGAAAGAGGTGCTTGACCTGCTTTATTTTTAAGGAAGCCTGAGGACTTTACTTAACTGGCGAATTGGTTACAAAGTAAGAAATGAGTTGTGTCAATTAAACTTCAGTGTATATTAGAGAATGTAAAGAATGTACCATGATTGGATTCTTACCTGCTACCTACAAATTTCCATCAATGACTATAGTTTTTCAGTGACTTGAGCTTTCTTTTGATTTCTCCTTTCCCCCCCCTGTAATATCCAGGGTGTAAAGGTTTCTCTCTCAGCAGTAACAGTTCCTGGTATCACAACTCTGGATTATGACATTTTGCACTTAATTTGGACAACAGAAAAGCTTCAGCAACAACTTGATGTGATTGACCAGCGCTATGATGTGTAACTATTTATGGCTCTTAACAGTTAGTTTATAAATTGTACTAAATTGTTCGAGTCATGGCTGCAAATGTATTAAATCCCATTGATGACATCAGAAGGCGTCTTAAGTAGTTCACGATGAGATAGGGGGTGAAAACATGAGCAATTCGGTATCTCGAGTGTTTGATTTCATCATTTACCAAAATGTTCACTTAGAATGTTGACCACGCCAACTTCTGGTTGTTAAAATAGAAGGCTTAATGGAAGTTTTCAGTGTTTGAGATTACGATGGGTGGAATGACTGTAATTATAATATAAAAGTCTCCTTCCAATATGCGTATGGAAGTATATCTTCCATATGGTGGGTTGTCTTACGAGATTAAACCAATTTTTCATTGCAGGTCGAGACAATCCGCATTAGCTTCTTTAAAGTCTGGAAACAAAAAAACTGCACTGAAACATGCAAGAGAGTTAAAGATCACCACAGAAAGTCGAGAAAAAGTTGCATCCCTCTTAAACAGAGTGGAGGAAGTCCTAAATGCTATTGCAGATGCCGAATCAACAAAAACGGTAGTTTACCATCTTTAGTTCTCTTTTTTGATTAATGGAAGGTGCTTTGCCCGTACCTTTGTCTTTCTAGTGGCTTGTTTGTTTACTTTCTACTTAGTGCTTAAGAACACTTTTTTAAGTGCTTGGAAAGTCATTCAACACAGGTCCTAAAAACAAGAGAGGCTTTTAGCTTGAAACCATTAAAAACAAATACGGATTTTGAGTTACTAAAAAAAATTGTTTGATATCATACTCTGTTGACAGGTTTCTGAGGCTATTCAAATAGGTGCCCGAGTAATGAAAGAACACGAGGTTAGTTGGGATCAAGTCCAGCACAGTTTGCAGGAACTAGAAGCAAGCATTGATGTACAAAAGCAAGTTGCAAGTGCTATAGGTACGTGTAGTCTGGATTTTCGCCCAGTTTCTCGTCTTTAGACGATTTTAGAAGTTCCTATTTACTTTGTTTAGTAACCAATTAATCTTCGACCTTCTCTTTCATTATGATTGAATATGGTTCTGGGAATCATAGATTCAGCTCCATCTGGCTTGATTCTGGAAGATGAAGATATTGAGGAGGAGTTTAAGAAGCTCGAGTTGGAAGTAACAGGCCAAAGCCTCGACGCGTCGACATCTGAATCTGGGGTTACTATTGCAACTGGTGAAACAGTGGCTACAGTTTCGGACGATTCATTGAGCACTGCATTATCAAATCTAAAGCTTGTGGAAGATACAGGAAAGGAGACAGTGAATCAGAAGTCAAATTCTAAGAACAAGTCGAAAATAATGGAGCTTGGCATTTCTTAG

mRNA sequence

ATGGAAAAGGAATCAAAGGGATCGTGTGTTAGAGAGTTCATCAGGGAAAAAGTCCCTGACTGGGATGACGAAGTGGTGGCTACAGCTCGGTTCAAGGCATTTAGTGGGCAAAAATCTGATTGGGAACCCAGATACCTATTTTGGAGGGATTTGATCCTCACAATTGCTCATCAATTCAACTTCATCTTCATTAAATCTTCTGAAATAAGGACTCAGTGGTTTTCTCGAGGAGGGTTGGCTCCACTGTGCCTTGACCATGTTCTGCATCTAATGTATATTGAGGGCGACATTATAAGACGTAGTGACATGTTGGACCCGAGGAGTGGCCAACTCTCTTACTTGTTTAAAAAACTAAGCAATTTGATGGGTACATCCAAAAAGAACGCCGATGATTTGATTCGCGATGATTATATAGTTCTTGCCTCTGTATTAAAGGATAGAGCAACTGAAGTCGTCAAGTGTTTATCTCAAAGTAATTGGACCTCTTCCTGCATTGTTACAATGGTGAAGTTCCAGAACCTCTGTGGAGGACCTGATGAAGCGACTGCCATCTTGAGTTACTTAATTGGATGTGGTAAAGCAAGGTATCTCTCTAGGGAAAAAAAGGATCTCTTAGAGGGTGTAAAGGTTTCTCTCTCAGCAGTAACAGTTCCTGGTATCACAACTCTGGATTATGACATTTTGCACTTAATTTGGACAACAGAAAAGCTTCAGCAACAACTTGATGTGATTGACCAGCGCTATGATGTGTCGAGACAATCCGCATTAGCTTCTTTAAAGTCTGGAAACAAAAAAACTGCACTGAAACATGCAAGAGAGTTAAAGATCACCACAGAAAGTCGAGAAAAAGTTGCATCCCTCTTAAACAGAGTGGAGGAAGTCCTAAATGCTATTGCAGATGCCGAATCAACAAAAACGGTTTCTGAGGCTATTCAAATAGGTGCCCGAGTAATGAAAGAACACGAGGTTAGTTGGGATCAAGTCCAGCACAGTTTGCAGGAACTAGAAGCAAGCATTGATGTACAAAAGCAAGTTGCAAGTGCTATAGATTCAGCTCCATCTGGCTTGATTCTGGAAGATGAAGATATTGAGGAGGAGTTTAAGAAGCTCGAGTTGGAAGTAACAGGCCAAAGCCTCGACGCGTCGACATCTGAATCTGGGGTTACTATTGCAACTGGTGAAACAGTGGCTACAGTTTCGGACGATTCATTGAGCACTGCATTATCAAATCTAAAGCTTGTGGAAGATACAGGAAAGGAGACAGTGAATCAGAAGTCAAATTCTAAGAACAAGTCGAAAATAATGGAGCTTGGCATTTCTTAG

Coding sequence (CDS)

ATGGAAAAGGAATCAAAGGGATCGTGTGTTAGAGAGTTCATCAGGGAAAAAGTCCCTGACTGGGATGACGAAGTGGTGGCTACAGCTCGGTTCAAGGCATTTAGTGGGCAAAAATCTGATTGGGAACCCAGATACCTATTTTGGAGGGATTTGATCCTCACAATTGCTCATCAATTCAACTTCATCTTCATTAAATCTTCTGAAATAAGGACTCAGTGGTTTTCTCGAGGAGGGTTGGCTCCACTGTGCCTTGACCATGTTCTGCATCTAATGTATATTGAGGGCGACATTATAAGACGTAGTGACATGTTGGACCCGAGGAGTGGCCAACTCTCTTACTTGTTTAAAAAACTAAGCAATTTGATGGGTACATCCAAAAAGAACGCCGATGATTTGATTCGCGATGATTATATAGTTCTTGCCTCTGTATTAAAGGATAGAGCAACTGAAGTCGTCAAGTGTTTATCTCAAAGTAATTGGACCTCTTCCTGCATTGTTACAATGGTGAAGTTCCAGAACCTCTGTGGAGGACCTGATGAAGCGACTGCCATCTTGAGTTACTTAATTGGATGTGGTAAAGCAAGGTATCTCTCTAGGGAAAAAAAGGATCTCTTAGAGGGTGTAAAGGTTTCTCTCTCAGCAGTAACAGTTCCTGGTATCACAACTCTGGATTATGACATTTTGCACTTAATTTGGACAACAGAAAAGCTTCAGCAACAACTTGATGTGATTGACCAGCGCTATGATGTGTCGAGACAATCCGCATTAGCTTCTTTAAAGTCTGGAAACAAAAAAACTGCACTGAAACATGCAAGAGAGTTAAAGATCACCACAGAAAGTCGAGAAAAAGTTGCATCCCTCTTAAACAGAGTGGAGGAAGTCCTAAATGCTATTGCAGATGCCGAATCAACAAAAACGGTTTCTGAGGCTATTCAAATAGGTGCCCGAGTAATGAAAGAACACGAGGTTAGTTGGGATCAAGTCCAGCACAGTTTGCAGGAACTAGAAGCAAGCATTGATGTACAAAAGCAAGTTGCAAGTGCTATAGATTCAGCTCCATCTGGCTTGATTCTGGAAGATGAAGATATTGAGGAGGAGTTTAAGAAGCTCGAGTTGGAAGTAACAGGCCAAAGCCTCGACGCGTCGACATCTGAATCTGGGGTTACTATTGCAACTGGTGAAACAGTGGCTACAGTTTCGGACGATTCATTGAGCACTGCATTATCAAATCTAAAGCTTGTGGAAGATACAGGAAAGGAGACAGTGAATCAGAAGTCAAATTCTAAGAACAAGTCGAAAATAATGGAGCTTGGCATTTCTTAG

Protein sequence

MEKESKGSCVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFNFIFIKSSEIRTQWFSRGGLAPLCLDHVLHLMYIEGDIIRRSDMLDPRSGQLSYLFKKLSNLMGTSKKNADDLIRDDYIVLASVLKDRATEVVKCLSQSNWTSSCIVTMVKFQNLCGGPDEATAILSYLIGCGKARYLSREKKDLLEGVKVSLSAVTVPGITTLDYDILHLIWTTEKLQQQLDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIADAESTKTVSEAIQIGARVMKEHEVSWDQVQHSLQELEASIDVQKQVASAIDSAPSGLILEDEDIEEEFKKLELEVTGQSLDASTSESGVTIATGETVATVSDDSLSTALSNLKLVEDTGKETVNQKSNSKNKSKIMELGIS
Homology
BLAST of Tan0011229 vs. ExPASy Swiss-Prot
Match: Q6PBQ2 (Charged multivesicular body protein 7 OS=Danio rerio OX=7955 GN=chmp7 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.8e-17
Identity = 89/377 (23.61%), Postives = 175/377 (46.42%), Query Frame = 0

Query: 19  PDWDDEVVATARFKAFSGQK----SDWEPRYLFWRDLILTIAHQFNFIFIKSSEIRTQWF 78
           PDWDD+   +  F AF   +    +DW+ +  FW  LI+    +   + +   ++    F
Sbjct: 14  PDWDDDERMSFLFSAFKENRDVDCTDWDGKIDFWSPLIIEHCRRCGSVCVNLQDLNEN-F 73

Query: 79  SRGGLAPLCLDHVLHLMYIEGDIIRRSDM-LDPRSGQLSY---------LFKKLSNLMGT 138
            R G  PL L  V+  M   G + + SD   +  SG LS+         L   LS L+G+
Sbjct: 74  RRKGSVPLGLSTVIQSMIRSGKVQKESDFAANVDSGWLSWGVGLLLVRPLKWTLSALLGS 133

Query: 139 SKKNADDLIRDDYIVLASVLKDRATEVVKCLSQSNWTSSCIVTMVKFQNLCGG--PDEAT 198
            +     +  ++  V+  ++K++A E++     S  ++  +++  + ++L     PDE+T
Sbjct: 134 GR-----VPLEESFVVIELVKEKAAELLAAYRGSALSARSLLSFQELRSLSSHICPDEST 193

Query: 199 AILSYLIGCGKARYLSREKK---DLLEGVKV----SLSAVTVPGITTLDYDILHLIWTTE 258
             ++ L        L REK     L EG K+          V  ++ +D  I  L  + +
Sbjct: 194 LCMALL-------QLQREKHVTVSLHEGEKLVKFSQAGQGRVSPVSEVDLGIYQLQCSEK 253

Query: 259 KLQQQLDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVL 318
            L+++++ +    +  +Q A + LK G K  AL+  R  K   +  +++ + L  V+ +L
Sbjct: 254 LLEERVEALGHEAEKCKQQAKSLLKEGKKSQALRCLRGSKRVEKKADRLFAQLETVKGIL 313

Query: 319 NAIADAESTKTVSEAIQIGARVMK--EHEVSWDQVQHSLQELEASIDVQKQVASAIDSAP 371
           + IA++++ + V +A Q G   ++     V+ ++ ++ + +++   D Q +V   + S  
Sbjct: 314 DRIANSQTDRLVMQAYQAGVAALRISLKGVTVERAENLVDQIQELCDTQDEVNQTLASGA 373

BLAST of Tan0011229 vs. ExPASy Swiss-Prot
Match: Q871Y8 (Vacuolar-sorting protein snf7 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=vsp-3 PE=3 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 6.5e-04
Identity = 44/169 (26.04%), Postives = 86/169 (50.89%), Query Frame = 0

Query: 237 LQQQLDVIDQR-----YDVSRQSALASLKSGNKKTALKHA-RELKITTESREKVASLLNR 296
           L+ QLD++ +R       +  Q A+A       KTA K A R  K+   + E     +  
Sbjct: 28  LRTQLDMLQKRERHLQNQIDEQDAIARKNVSTNKTAAKQALRRKKVAESTLETTLGQITT 87

Query: 297 VEEVLNAIADAESTKTVSEAIQIGARVM-KEH-EVSWDQVQHSLQELEASIDVQKQVASA 356
           +E+ +NAI  A   +    A+Q     M K H +++ ++V   + +L+ + D+  ++A+A
Sbjct: 88  LEQQINAIESANINRETLAAMQAAREAMGKIHGKLTPEKVDEEMAKLQEANDLSNEIATA 147

Query: 357 IDSAPSGLILEDEDIEEEFKKLELEVTGQSLDASTSESGVTIATGETVA 398
           I SA  G  +++ ++E+E +KL+ E     +D+   E+G +I   + ++
Sbjct: 148 ITSANIGQPIDEGELEDELEKLQQE----EVDSKLHETGGSIPVHDKIS 192

BLAST of Tan0011229 vs. NCBI nr
Match: KAG6579154.1 (Charged multivesicular body protein 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016673.1 Charged multivesicular body protein 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 744.2 bits (1920), Expect = 6.5e-211
Identity = 386/437 (88.33%), Postives = 410/437 (93.82%), Query Frame = 0

Query: 1   MEKESKGSCVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN 60
           MEKESK   VREFIREKVPDWD+E+VATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN
Sbjct: 1   MEKESKELLVREFIREKVPDWDNELVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN 60

Query: 61  FIFIKSSEIRTQWFSRGGLAPLCLDHVLHLMYIEGDIIRRSDMLDPRSGQLSYLFKKLSN 120
           FIF+K SEI+ QWFSRGGLAPLCLDHVLHLM IEGDIIRRSDMLDPR GQLSYLFKKLSN
Sbjct: 61  FIFLKPSEIKNQWFSRGGLAPLCLDHVLHLMQIEGDIIRRSDMLDPRGGQLSYLFKKLSN 120

Query: 121 LMGTSKKNADDLIRDDYIVLASVLKDRATEVVKCLSQSNWTSSCIVTMVKFQNLCGGPDE 180
           LMGTSKKN D  + DDYIVLA VL+DRA EVVKCLS SNWTSSC++TMVKFQN+CGGPDE
Sbjct: 121 LMGTSKKNLDGFLSDDYIVLACVLQDRAAEVVKCLSHSNWTSSCVITMVKFQNICGGPDE 180

Query: 181 ATAILSYLIGCGKARYLSREKKDLLEGVKVSLSAVTVPGITTLDYDILHLIWTTEKLQQQ 240
           ATAILSYL  CGKARYLS+E+K+L+EGVK+SLSA  VPGITTLDYDILHLIWTTE+LQ+Q
Sbjct: 181 ATAILSYLTECGKARYLSKEQKELVEGVKLSLSAAKVPGITTLDYDILHLIWTTERLQRQ 240

Query: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD 300
           LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD
Sbjct: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD 300

Query: 301 AESTKTVSEAIQIGARVMKEHEVSWDQVQHSLQELEASIDVQKQVASAIDSAPSGLILED 360
           AESTKTVSEAIQIGARVMKEHEVSWD +QHSLQELEASID+QKQVAS IDSAPSG ILE+
Sbjct: 301 AESTKTVSEAIQIGARVMKEHEVSWDILQHSLQELEASIDIQKQVASVIDSAPSGSILEE 360

Query: 361 EDIEEEFKKLELEVTGQSLDASTSESGVTIATGETVATVSDDSLSTALSNLKLVEDTGKE 420
           EDIEEEFKKLELEV GQ+LDASTS++GV IATG  VATVSDDSLS ALSNLKLVE+TGKE
Sbjct: 361 EDIEEEFKKLELEVAGQNLDASTSDTGVNIATGNQVATVSDDSLSAALSNLKLVEETGKE 420

Query: 421 TVNQKSNSKNKSKIMEL 438
           TV QKSNSK+KSKIMEL
Sbjct: 421 TVIQKSNSKSKSKIMEL 437

BLAST of Tan0011229 vs. NCBI nr
Match: XP_023551290.1 (charged multivesicular body protein 7 [Cucurbita pepo subsp. pepo] >XP_023551291.1 charged multivesicular body protein 7 [Cucurbita pepo subsp. pepo] >XP_023551292.1 charged multivesicular body protein 7 [Cucurbita pepo subsp. pepo] >XP_023551293.1 charged multivesicular body protein 7 [Cucurbita pepo subsp. pepo] >XP_023551294.1 charged multivesicular body protein 7 [Cucurbita pepo subsp. pepo] >XP_023551295.1 charged multivesicular body protein 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 743.4 bits (1918), Expect = 1.1e-210
Identity = 385/437 (88.10%), Postives = 410/437 (93.82%), Query Frame = 0

Query: 1   MEKESKGSCVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN 60
           MEKESK   VREFIREKVPDWD+EVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN
Sbjct: 1   MEKESKELLVREFIREKVPDWDNEVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN 60

Query: 61  FIFIKSSEIRTQWFSRGGLAPLCLDHVLHLMYIEGDIIRRSDMLDPRSGQLSYLFKKLSN 120
           FIF+K SEI+ QWFSRGGLAPLCLDHVLHLM IEGDIIRRSDMLDPR GQLSYLFKKLSN
Sbjct: 61  FIFLKPSEIKNQWFSRGGLAPLCLDHVLHLMQIEGDIIRRSDMLDPRGGQLSYLFKKLSN 120

Query: 121 LMGTSKKNADDLIRDDYIVLASVLKDRATEVVKCLSQSNWTSSCIVTMVKFQNLCGGPDE 180
           LMGTSKKN D L+ DDYI LA VL+DRA EVVKCLS SNWTSSC++TMVKFQN+CGGPDE
Sbjct: 121 LMGTSKKNPDSLLSDDYIALARVLQDRAAEVVKCLSHSNWTSSCVITMVKFQNICGGPDE 180

Query: 181 ATAILSYLIGCGKARYLSREKKDLLEGVKVSLSAVTVPGITTLDYDILHLIWTTEKLQQQ 240
           ATAILSYL  CGKARYLS+E+K+L+EGVK+SLSA  VPGITTLDYDILHLIWTTE+LQ+Q
Sbjct: 181 ATAILSYLTECGKARYLSKEQKELVEGVKLSLSAAKVPGITTLDYDILHLIWTTERLQRQ 240

Query: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD 300
           LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESR+KVASLLNRVEEVLNAIAD
Sbjct: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESRKKVASLLNRVEEVLNAIAD 300

Query: 301 AESTKTVSEAIQIGARVMKEHEVSWDQVQHSLQELEASIDVQKQVASAIDSAPSGLILED 360
           AESTKTVSEAIQIGARVMKEHEVSWDQ+QHSLQELEASID+QKQVAS IDSAPSG ILE+
Sbjct: 301 AESTKTVSEAIQIGARVMKEHEVSWDQLQHSLQELEASIDIQKQVASVIDSAPSGSILEE 360

Query: 361 EDIEEEFKKLELEVTGQSLDASTSESGVTIATGETVATVSDDSLSTALSNLKLVEDTGKE 420
           EDI+EEFKKLELEV GQ+LDASTS++GV IATG  VATVSDDSLS ALSNLKLVE+TGKE
Sbjct: 361 EDIKEEFKKLELEVAGQNLDASTSDTGVNIATGNQVATVSDDSLSAALSNLKLVEETGKE 420

Query: 421 TVNQKSNSKNKSKIMEL 438
           TV QKSNSK+K KIMEL
Sbjct: 421 TVIQKSNSKSKLKIMEL 437

BLAST of Tan0011229 vs. NCBI nr
Match: XP_022938857.1 (charged multivesicular body protein 7 [Cucurbita moschata] >XP_022938858.1 charged multivesicular body protein 7 [Cucurbita moschata] >XP_022938859.1 charged multivesicular body protein 7 [Cucurbita moschata] >XP_022938860.1 charged multivesicular body protein 7 [Cucurbita moschata] >XP_022938861.1 charged multivesicular body protein 7 [Cucurbita moschata])

HSP 1 Score: 743.0 bits (1917), Expect = 1.4e-210
Identity = 385/437 (88.10%), Postives = 409/437 (93.59%), Query Frame = 0

Query: 1   MEKESKGSCVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN 60
           MEKESK   VREFIREKVPDWD+E+VATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN
Sbjct: 1   MEKESKELLVREFIREKVPDWDNELVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN 60

Query: 61  FIFIKSSEIRTQWFSRGGLAPLCLDHVLHLMYIEGDIIRRSDMLDPRSGQLSYLFKKLSN 120
           FIF+K SEI+ QWFSRGGLAPLCLDHVLHLM IEGDIIRRSDMLDPR GQLSYLFKKLSN
Sbjct: 61  FIFLKPSEIKNQWFSRGGLAPLCLDHVLHLMQIEGDIIRRSDMLDPRGGQLSYLFKKLSN 120

Query: 121 LMGTSKKNADDLIRDDYIVLASVLKDRATEVVKCLSQSNWTSSCIVTMVKFQNLCGGPDE 180
           LMGTSKKN D L+ DDYIVLA VL+DRA EVVKCLS SNWTSSC++TMVKFQN+CGGPDE
Sbjct: 121 LMGTSKKNLDGLLSDDYIVLACVLQDRAAEVVKCLSHSNWTSSCVITMVKFQNICGGPDE 180

Query: 181 ATAILSYLIGCGKARYLSREKKDLLEGVKVSLSAVTVPGITTLDYDILHLIWTTEKLQQQ 240
           ATA LSYL  CGKARYLS+E+K+L+EGVK+SLSA  VPGITTLDYDILHLIWTTE+LQ+Q
Sbjct: 181 ATATLSYLTECGKARYLSKEQKELVEGVKLSLSAAKVPGITTLDYDILHLIWTTERLQRQ 240

Query: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD 300
           LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD
Sbjct: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD 300

Query: 301 AESTKTVSEAIQIGARVMKEHEVSWDQVQHSLQELEASIDVQKQVASAIDSAPSGLILED 360
           AESTKTVSEAIQIGAR MKEHEVSWD +QHSLQELEASID+QKQVAS IDSAPSGLILE+
Sbjct: 301 AESTKTVSEAIQIGARAMKEHEVSWDILQHSLQELEASIDIQKQVASVIDSAPSGLILEE 360

Query: 361 EDIEEEFKKLELEVTGQSLDASTSESGVTIATGETVATVSDDSLSTALSNLKLVEDTGKE 420
           EDIEEEFKKLELEV GQ+LDASTS++G  IATG  VATVSDDSLS ALSNLKLVE+TGKE
Sbjct: 361 EDIEEEFKKLELEVAGQNLDASTSDTGANIATGNQVATVSDDSLSAALSNLKLVEETGKE 420

Query: 421 TVNQKSNSKNKSKIMEL 438
           TV QKSNSK+KSKIMEL
Sbjct: 421 TVIQKSNSKSKSKIMEL 437

BLAST of Tan0011229 vs. NCBI nr
Match: XP_038875996.1 (uncharacterized protein LOC120068336 isoform X2 [Benincasa hispida])

HSP 1 Score: 740.0 bits (1909), Expect = 1.2e-209
Identity = 390/443 (88.04%), Postives = 414/443 (93.45%), Query Frame = 0

Query: 1   MEKESKGSCVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN 60
           MEKESKGS VREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRY  WRDLI+TIA +FN
Sbjct: 1   MEKESKGSRVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYQVWRDLIITIARKFN 60

Query: 61  FIFIKSSEIRTQWFSRGGLAPLCLDHVLHLMYIEGDIIRRSDMLDPRSGQLSYLFKKLSN 120
           FIFIK SEI+ QWFSRGGL+PLCLDHVLH+MYIEGDIIRR DMLDPRSGQLSYLFKKLSN
Sbjct: 61  FIFIKPSEIKNQWFSRGGLSPLCLDHVLHVMYIEGDIIRRGDMLDPRSGQLSYLFKKLSN 120

Query: 121 LMGTSKKNADDLIRDDYIVLASVLKDRATEVVKCLSQSNWTSSCIVTMVKFQNLCGGPDE 180
           LMGTSKKN D L+RDDY+VLA VL+DRA EV+KCLS SNWTSSCI+TMVKFQN+CGGPDE
Sbjct: 121 LMGTSKKNPDSLLRDDYVVLACVLQDRAAEVIKCLSLSNWTSSCIITMVKFQNICGGPDE 180

Query: 181 ATAILSYLIGCGKARYLSREKKDLLEGVKVSLSAVTVPGITTLDYDILHLIWTTEKLQQQ 240
           AT ILSYLIG GKARYLS+EKK+LLEGVK+SL+A+TVPGITTLDYDILHLIWTTEKLQQQ
Sbjct: 181 ATVILSYLIGYGKARYLSKEKKELLEGVKISLTAMTVPGITTLDYDILHLIWTTEKLQQQ 240

Query: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD 300
           LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD
Sbjct: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD 300

Query: 301 AESTKTVSEAIQIGARVMKEHEVSWDQVQHSLQELEASIDVQKQVASAI--DSAPSGLIL 360
           AESTKTVSEAIQIGARVMKEHEVSWDQ+Q+SLQE+E SID+QKQVASAI  DSAPSG I 
Sbjct: 301 AESTKTVSEAIQIGARVMKEHEVSWDQLQYSLQEIEVSIDLQKQVASAISTDSAPSGSIP 360

Query: 361 EDEDIEEEFKKLELEVT-GQSLDASTSESGVTIATGETVATVSDDSLSTALSNLKLVEDT 420
           EDEDIEEEFKKLELEVT GQ+LD STSES V IATGETVATVSDD LS ALSNLKLVE+T
Sbjct: 361 EDEDIEEEFKKLELEVTAGQNLDPSTSESVVNIATGETVATVSDDLLSDALSNLKLVEET 420

Query: 421 GKETVNQKSNSKNKSKIMELGIS 441
           G  T  QKSNSK+KSK+MELGIS
Sbjct: 421 GNVTAIQKSNSKSKSKMMELGIS 443

BLAST of Tan0011229 vs. NCBI nr
Match: XP_038875993.1 (uncharacterized protein LOC120068336 isoform X1 [Benincasa hispida] >XP_038875994.1 uncharacterized protein LOC120068336 isoform X1 [Benincasa hispida] >XP_038875995.1 uncharacterized protein LOC120068336 isoform X1 [Benincasa hispida])

HSP 1 Score: 733.8 bits (1893), Expect = 8.7e-208
Identity = 391/452 (86.50%), Postives = 414/452 (91.59%), Query Frame = 0

Query: 1   MEKESKGSCVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN 60
           MEKESKGS VREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRY  WRDLI+TIA +FN
Sbjct: 1   MEKESKGSRVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYQVWRDLIITIARKFN 60

Query: 61  FIFIKSSEIRTQWFSRGGLAPLCLDHVLHLMYIEGDIIRRSDMLDPRSGQLSYLFKKLSN 120
           FIFIK SEI+ QWFSRGGL+PLCLDHVLH+MYIEGDIIRR DMLDPRSGQLSYLFKKLSN
Sbjct: 61  FIFIKPSEIKNQWFSRGGLSPLCLDHVLHVMYIEGDIIRRGDMLDPRSGQLSYLFKKLSN 120

Query: 121 LMGTSKKNADDLIRDDYIVLASVL---------KDRATEVVKCLSQSNWTSSCIVTMVKF 180
           LMGTSKKN D L+RDDY+VLA VL         KDRA EV+KCLS SNWTSSCI+TMVKF
Sbjct: 121 LMGTSKKNPDSLLRDDYVVLACVLQFDMKYFHAKDRAAEVIKCLSLSNWTSSCIITMVKF 180

Query: 181 QNLCGGPDEATAILSYLIGCGKARYLSREKKDLLEGVKVSLSAVTVPGITTLDYDILHLI 240
           QN+CGGPDEAT ILSYLIG GKARYLS+EKK+LLEGVK+SL+A+TVPGITTLDYDILHLI
Sbjct: 181 QNICGGPDEATVILSYLIGYGKARYLSKEKKELLEGVKISLTAMTVPGITTLDYDILHLI 240

Query: 241 WTTEKLQQQLDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRV 300
           WTTEKLQQQLDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRV
Sbjct: 241 WTTEKLQQQLDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRV 300

Query: 301 EEVLNAIADAESTKTVSEAIQIGARVMKEHEVSWDQVQHSLQELEASIDVQKQVASAI-- 360
           EEVLNAIADAESTKTVSEAIQIGARVMKEHEVSWDQ+Q+SLQE+E SID+QKQVASAI  
Sbjct: 301 EEVLNAIADAESTKTVSEAIQIGARVMKEHEVSWDQLQYSLQEIEVSIDLQKQVASAIST 360

Query: 361 DSAPSGLILEDEDIEEEFKKLELEVT-GQSLDASTSESGVTIATGETVATVSDDSLSTAL 420
           DSAPSG I EDEDIEEEFKKLELEVT GQ+LD STSES V IATGETVATVSDD LS AL
Sbjct: 361 DSAPSGSIPEDEDIEEEFKKLELEVTAGQNLDPSTSESVVNIATGETVATVSDDLLSDAL 420

Query: 421 SNLKLVEDTGKETVNQKSNSKNKSKIMELGIS 441
           SNLKLVE+TG  T  QKSNSK+KSK+MELGIS
Sbjct: 421 SNLKLVEETGNVTAIQKSNSKSKSKMMELGIS 452

BLAST of Tan0011229 vs. ExPASy TrEMBL
Match: A0A6J1FF90 (charged multivesicular body protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111444939 PE=4 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 7.0e-211
Identity = 385/437 (88.10%), Postives = 409/437 (93.59%), Query Frame = 0

Query: 1   MEKESKGSCVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN 60
           MEKESK   VREFIREKVPDWD+E+VATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN
Sbjct: 1   MEKESKELLVREFIREKVPDWDNELVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN 60

Query: 61  FIFIKSSEIRTQWFSRGGLAPLCLDHVLHLMYIEGDIIRRSDMLDPRSGQLSYLFKKLSN 120
           FIF+K SEI+ QWFSRGGLAPLCLDHVLHLM IEGDIIRRSDMLDPR GQLSYLFKKLSN
Sbjct: 61  FIFLKPSEIKNQWFSRGGLAPLCLDHVLHLMQIEGDIIRRSDMLDPRGGQLSYLFKKLSN 120

Query: 121 LMGTSKKNADDLIRDDYIVLASVLKDRATEVVKCLSQSNWTSSCIVTMVKFQNLCGGPDE 180
           LMGTSKKN D L+ DDYIVLA VL+DRA EVVKCLS SNWTSSC++TMVKFQN+CGGPDE
Sbjct: 121 LMGTSKKNLDGLLSDDYIVLACVLQDRAAEVVKCLSHSNWTSSCVITMVKFQNICGGPDE 180

Query: 181 ATAILSYLIGCGKARYLSREKKDLLEGVKVSLSAVTVPGITTLDYDILHLIWTTEKLQQQ 240
           ATA LSYL  CGKARYLS+E+K+L+EGVK+SLSA  VPGITTLDYDILHLIWTTE+LQ+Q
Sbjct: 181 ATATLSYLTECGKARYLSKEQKELVEGVKLSLSAAKVPGITTLDYDILHLIWTTERLQRQ 240

Query: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD 300
           LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD
Sbjct: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD 300

Query: 301 AESTKTVSEAIQIGARVMKEHEVSWDQVQHSLQELEASIDVQKQVASAIDSAPSGLILED 360
           AESTKTVSEAIQIGAR MKEHEVSWD +QHSLQELEASID+QKQVAS IDSAPSGLILE+
Sbjct: 301 AESTKTVSEAIQIGARAMKEHEVSWDILQHSLQELEASIDIQKQVASVIDSAPSGLILEE 360

Query: 361 EDIEEEFKKLELEVTGQSLDASTSESGVTIATGETVATVSDDSLSTALSNLKLVEDTGKE 420
           EDIEEEFKKLELEV GQ+LDASTS++G  IATG  VATVSDDSLS ALSNLKLVE+TGKE
Sbjct: 361 EDIEEEFKKLELEVAGQNLDASTSDTGANIATGNQVATVSDDSLSAALSNLKLVEETGKE 420

Query: 421 TVNQKSNSKNKSKIMEL 438
           TV QKSNSK+KSKIMEL
Sbjct: 421 TVIQKSNSKSKSKIMEL 437

BLAST of Tan0011229 vs. ExPASy TrEMBL
Match: A0A6J1K252 (charged multivesicular body protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111489435 PE=4 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 1.2e-207
Identity = 381/437 (87.19%), Postives = 406/437 (92.91%), Query Frame = 0

Query: 1   MEKESKGSCVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN 60
           MEKESK   VREFIREKVPDWD+EVVATA FKAFSGQKSDWEPRYLFWRDLIL I+HQFN
Sbjct: 1   MEKESKELLVREFIREKVPDWDNEVVATAWFKAFSGQKSDWEPRYLFWRDLILKISHQFN 60

Query: 61  FIFIKSSEIRTQWFSRGGLAPLCLDHVLHLMYIEGDIIRRSDMLDPRSGQLSYLFKKLSN 120
           FIFIK SEI+ QWFSRGGLAPLCLDHVLHLM IEGDIIRRSDMLDPR GQLSYLFKKLSN
Sbjct: 61  FIFIKPSEIKNQWFSRGGLAPLCLDHVLHLMQIEGDIIRRSDMLDPRGGQLSYLFKKLSN 120

Query: 121 LMGTSKKNADDLIRDDYIVLASVLKDRATEVVKCLSQSNWTSSCIVTMVKFQNLCGGPDE 180
           +MGTSKKN D L+ DDYIVLA VL+DRA EVVKCLS SNWTSSC++TMVKFQN+CGGPDE
Sbjct: 121 MMGTSKKNPDGLLSDDYIVLACVLQDRAAEVVKCLSHSNWTSSCVITMVKFQNICGGPDE 180

Query: 181 ATAILSYLIGCGKARYLSREKKDLLEGVKVSLSAVTVPGITTLDYDILHLIWTTEKLQQQ 240
           ATAILSYL  CGKARYLS+E+K+L+EGVK+SLSA  VPGITTLDYDILHLIWTTE+LQ+Q
Sbjct: 181 ATAILSYLTECGKARYLSKEQKELVEGVKLSLSAAKVPGITTLDYDILHLIWTTERLQRQ 240

Query: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD 300
           LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD
Sbjct: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD 300

Query: 301 AESTKTVSEAIQIGARVMKEHEVSWDQVQHSLQELEASIDVQKQVASAIDSAPSGLILED 360
           AESTKTVSEAIQIGARVMKEHEVSWDQ+QHSL ELEASID+QKQV S IDSAPSG ILE+
Sbjct: 301 AESTKTVSEAIQIGARVMKEHEVSWDQLQHSLHELEASIDIQKQVESVIDSAPSGSILEE 360

Query: 361 EDIEEEFKKLELEVTGQSLDASTSESGVTIATGETVATVSDDSLSTALSNLKLVEDTGKE 420
           EDIEEEFKKLELEV GQ+LDA+TS++GV IATG  VATVSDDSLS ALSNLKLV +T KE
Sbjct: 361 EDIEEEFKKLELEVAGQNLDATTSDTGVNIATGHQVATVSDDSLSAALSNLKLVGETVKE 420

Query: 421 TVNQKSNSKNKSKIMEL 438
           TV QKSNSK+KSKIMEL
Sbjct: 421 TVIQKSNSKSKSKIMEL 437

BLAST of Tan0011229 vs. ExPASy TrEMBL
Match: A0A0A0KMY2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G118690 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 4.4e-205
Identity = 377/441 (85.49%), Postives = 405/441 (91.84%), Query Frame = 0

Query: 1   MEKESKGSCVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN 60
           MEKESKGSCVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILT+A QFN
Sbjct: 1   MEKESKGSCVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTVARQFN 60

Query: 61  FIFIKSSEIRTQWFSRGGLAPLCLDHVLHLMYIEGDIIRRSDMLDPRSGQLSYLFKKLSN 120
           F+ IK SEI+ QWF RGGL PLCLDHVLHLMY  GDIIRRSDMLDPRSGQLSY+FKKLSN
Sbjct: 61  FLIIKPSEIKNQWFYRGGLTPLCLDHVLHLMYTGGDIIRRSDMLDPRSGQLSYMFKKLSN 120

Query: 121 LMGTSKKNADDLIRDDYIVLASVLKDRATEVVKCLSQSNWTSSCIVTMVKFQNLCGGPDE 180
           LMGTSKKN D L+RDDYIVLA VL+DRA EV+KCLS S+WTSSCI+TMVKFQN+CGGPDE
Sbjct: 121 LMGTSKKNPDSLLRDDYIVLACVLQDRAAEVIKCLSLSSWTSSCIITMVKFQNICGGPDE 180

Query: 181 ATAILSYLIGCGKARYLSREKKDLLEGVKVSLSAVTVPGITTLDYDILHLIWTTEKLQQQ 240
           AT ILSYLI CGKA++LS+EKK+LLEGVKVSLSA TVPGIT+LDYDILHL+WT EKLQQQ
Sbjct: 181 ATVILSYLIECGKAKFLSKEKKELLEGVKVSLSATTVPGITSLDYDILHLVWTAEKLQQQ 240

Query: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD 300
           LDVIDQRYDVS+QSAL SLKSGN+KTALKHARELKITTESREKVASL NRVEEVLNAIAD
Sbjct: 241 LDVIDQRYDVSKQSALVSLKSGNRKTALKHARELKITTESREKVASLFNRVEEVLNAIAD 300

Query: 301 AESTKTVSEAIQIGARVMKEHEVSWDQVQHSLQELEASIDVQKQVASAIDSAPSGLILED 360
           AE TKTVSEAIQIGARVMKEHEV+WDQ+Q SLQELEAS+D+QKQVA+AIDS PS  I +D
Sbjct: 301 AELTKTVSEAIQIGARVMKEHEVNWDQLQDSLQELEASVDIQKQVANAIDSVPSSSIPDD 360

Query: 361 EDIEEEFKKLELEVT-GQSLDASTSESGVTIATGETVATVSDDSLSTALSNLKLVEDTGK 420
           EDIEEEFKKLELE+T GQ LDASTSESGV IATGETVA V DDSLSTALSNLKLVE+T K
Sbjct: 361 EDIEEEFKKLELELTAGQILDASTSESGVNIATGETVAAVCDDSLSTALSNLKLVEETEK 420

Query: 421 ETVNQKSNSKNKSKIMELGIS 441
           E  N  S+SK KSKIME+GIS
Sbjct: 421 ENGN-SSHSKRKSKIMEVGIS 440

BLAST of Tan0011229 vs. ExPASy TrEMBL
Match: A0A6J1CX81 (charged multivesicular body protein 7 OS=Momordica charantia OX=3673 GN=LOC111015645 PE=4 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 7.7e-202
Identity = 374/441 (84.81%), Postives = 402/441 (91.16%), Query Frame = 0

Query: 1   MEKESKGSCVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN 60
           MEKE KGS VREFI++KV DWDDEVV TARFKAFSGQKSDWEPRYLFWRDLILTIAH+FN
Sbjct: 1   MEKELKGSHVREFIKDKVTDWDDEVVTTARFKAFSGQKSDWEPRYLFWRDLILTIAHKFN 60

Query: 61  FIFIKSSEIRTQWFSRGGLAPLCLDHVLHLMYIEGDIIRRSDMLDPRSGQLSYLFKKLSN 120
           FIFIK  EIR QWFS+GGLAPLCLDHVLH MYI+GDII +SDMLDPRSGQLS+LFKKLSN
Sbjct: 61  FIFIKPIEIRNQWFSQGGLAPLCLDHVLHQMYIDGDIIIQSDMLDPRSGQLSHLFKKLSN 120

Query: 121 LMGTSKKNADDLIRDDYIVLASVLKDRATEVVKCLSQSNWTSSCIVTMVKFQNLCGGPDE 180
           LMGTSKKN +DL+RD+Y+VLASVLKDRA EVVKCLS SNWTSSCI+TM+KFQN+CGGPDE
Sbjct: 121 LMGTSKKNPNDLLRDEYVVLASVLKDRAAEVVKCLSHSNWTSSCIITMMKFQNICGGPDE 180

Query: 181 ATAILSYLIGCGKARYLSREKKDLLEGVKVSLSAVTVPGITTLDYDILHLIWTTEKLQQQ 240
           ATAILSYL GCGKARYLSREK++ LEGVKVSLSA TVP ITTLD DILHLIWTTEKLQQQ
Sbjct: 181 ATAILSYLFGCGKARYLSREKEEFLEGVKVSLSAATVPSITTLDCDILHLIWTTEKLQQQ 240

Query: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD 300
           LDVIDQRY+VSRQSALASLKSGNKKTALKHARELKI+TESREKVASLLNRVEEVLNAIAD
Sbjct: 241 LDVIDQRYNVSRQSALASLKSGNKKTALKHARELKISTESREKVASLLNRVEEVLNAIAD 300

Query: 301 AESTKTVSEAIQIGARVMKEHEVSWDQVQHSLQELEASIDVQKQVASAIDSAPSGLILED 360
           AESTKTVSEAIQIGARVMKEHEVSW+++QHS+QELE SID+ KQVASAIDSAPSG ILED
Sbjct: 301 AESTKTVSEAIQIGARVMKEHEVSWEELQHSMQELEDSIDLHKQVASAIDSAPSGSILED 360

Query: 361 EDIEEEFKKLELEVTGQSLDASTSESGVTIATGETVATVSDDSLSTALSNLKLVEDTGKE 420
           EDIEEEF+K ELEVTGQ++D  T  SG         A+VSDD LS ALSNLKLVEDTGKE
Sbjct: 361 EDIEEEFRKFELEVTGQNIDVPTPNSG---------ASVSDDLLSIALSNLKLVEDTGKE 420

Query: 421 -TVNQKSNSKNKSKIMELGIS 441
            TVNQ SNS +KSKIMELGIS
Sbjct: 421 TTVNQNSNSNSKSKIMELGIS 432

BLAST of Tan0011229 vs. ExPASy TrEMBL
Match: A0A1S3CRA4 (charged multivesicular body protein 7 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503835 PE=4 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 2.2e-196
Identity = 364/442 (82.35%), Postives = 390/442 (88.24%), Query Frame = 0

Query: 1   MEKESKGSCVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFN 60
           MEKESKGSCVREFIREKV DWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILT+A Q N
Sbjct: 1   MEKESKGSCVREFIREKVLDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTVARQLN 60

Query: 61  FIFIKSSEIRTQWFSRGGLAPLCLDHVLHLMYIEGDIIRRSDMLDPRSGQLSYLFKKLSN 120
           F+ IK SEI+ QWFSRGGL PLCLDHVLHLMY  GDIIRRSDMLDPRSGQLSY+FK+LSN
Sbjct: 61  FLIIKPSEIKNQWFSRGGLTPLCLDHVLHLMYTGGDIIRRSDMLDPRSGQLSYMFKRLSN 120

Query: 121 LMGTSKKNADDLIRDDYIVLASVLKDRATEVVKCLSQSNWTSSCIVTMVKFQNLCGGPDE 180
           LMGTSKKN + L+RDDYI+LA VL+DRATEV+KCLS SNWTSS I+TMVKFQN+CGGPDE
Sbjct: 121 LMGTSKKNPESLLRDDYIILACVLQDRATEVIKCLSLSNWTSSYIITMVKFQNICGGPDE 180

Query: 181 ATAILSYLIGCGKARYLSREKKDLLEGVKVSLSAVTVPGITTLDYDILHLIWTTEKLQQQ 240
           AT ILSYLI CGKA++LS+ K  LLEGVKVS SA TVPGITTLDYDILHL+WT EKLQQQ
Sbjct: 181 ATVILSYLIECGKAKFLSKGKTKLLEGVKVSFSATTVPGITTLDYDILHLVWTAEKLQQQ 240

Query: 241 LDVIDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIAD 300
           LD I+QRYDVS+QSAL SLKSGNKK ALKHARELKITTESREKVASL NRVEEVLNAI D
Sbjct: 241 LDAINQRYDVSKQSALVSLKSGNKKAALKHARELKITTESREKVASLFNRVEEVLNAIGD 300

Query: 301 AESTKTVSEAIQIGARVMKEHEVSWDQVQHSLQELEASIDVQKQVASAIDSAPSGLILED 360
           AE TK+VSEAIQIGARVMKEHEV+WDQ+QHSLQELE SID+QKQVA+ IDS PS  I  D
Sbjct: 301 AELTKSVSEAIQIGARVMKEHEVNWDQLQHSLQELETSIDIQKQVANTIDSVPSASIPND 360

Query: 361 -EDIEEEFKKLELEVT-GQSLDASTSESGVTIATGETVATVSDDSLSTALSNLKLVEDTG 420
            EDIEE FKKLELE+T  Q LDASTSES V IATGETV  V DDSLS+ LSNLKLVE+  
Sbjct: 361 EEDIEEVFKKLELELTAAQILDASTSESAVNIATGETVVVVCDDSLSSTLSNLKLVEEVE 420

Query: 421 KETVNQKSNSKNKSKIMELGIS 441
           KE  NQKSNSK  SKIMELGIS
Sbjct: 421 KEDANQKSNSKRNSKIMELGIS 442

BLAST of Tan0011229 vs. TAIR 10
Match: AT3G62080.2 (SNF7 family protein )

HSP 1 Score: 413.3 bits (1061), Expect = 2.4e-115
Identity = 226/432 (52.31%), Postives = 309/432 (71.53%), Query Frame = 0

Query: 4   ESKGSCVREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFNFIF 63
           E     V+EFIR +VPDWDDEVVA ARFKAFSGQ+SDWE ++ FWRDLI+ ++ QF    
Sbjct: 39  EMDPEAVKEFIRREVPDWDDEVVAMARFKAFSGQRSDWELKFQFWRDLIIKVSRQFGLFI 98

Query: 64  IKSSEIRTQWFSRGGLAPLCLDHVLHLMYIEGDIIRRSDMLDPRSGQLSYLFKKLSNLMG 123
           I   +++  WF RGG+ PLC+D V+ LM+ EGD++R SD+ DP SG+++ L + + NLM 
Sbjct: 99  IDPVQVKKAWFDRGGMTPLCIDDVVLLMHSEGDVVRISDLDDPGSGRIARLLRTVKNLMV 158

Query: 124 TSKKNADDLIRDDYIVLASVLKDRATEVVKCLSQSNWTSSCIVTMVKFQNLCGGPDEATA 183
                 ++++ ++ +VL  +LK++A +VVK LS+ +WTS+C+VT+ KF+NLC G +EA+A
Sbjct: 159 QQPVKQEEIL-ENTLVLVPLLKEKAADVVKILSEGHWTSTCVVTLKKFRNLCNGSNEASA 218

Query: 184 ILSYLIGCGKARYLSREKKDLLEGVKVSLSAVTVPGITTLDYDILHLIWTTEKLQQQLDV 243
           +LS+L GCGKA  +S  + +L+EGVKVS S   +PGI+TLD DILHL+ TTEKLQ QL+V
Sbjct: 219 VLSHLSGCGKAHKISINRGELIEGVKVSFSQTALPGISTLDCDILHLLRTTEKLQDQLEV 278

Query: 244 IDQRYDVSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIADAES 303
           +DQR + S++SALASLKSG++K AL+HARELK+ TESREK  SLLNRVEEVLN IAD+ES
Sbjct: 279 MDQRCEKSKKSALASLKSGHRKVALRHARELKVVTESREKCTSLLNRVEEVLNTIADSES 338

Query: 304 TKTVSEAIQIGARVMKEHEVSWDQVQHSLQELEASIDVQKQVASAIDSAPSGLILEDEDI 363
           TK VSEAI+ GARVMK+ ++S D V   L+ELE +I+ QKQV  A++SAP   I +DEDI
Sbjct: 339 TKMVSEAIKTGARVMKDIKISADDVHDYLEELEETIESQKQVEKALESAPYPDI-DDEDI 398

Query: 364 EEEFKKLELEVTGQSLDASTSESGVTIATGETVATVSDDSLSTALSNLKL--VEDTGKET 423
           EEE  +LE+++  +S       S V  AT +T      DSL+   S LKL   + T +E 
Sbjct: 399 EEELLELEMDLESES-------SQVLPATSDTA-----DSLTEMFSELKLGKTKQTLEEQ 456

Query: 424 VNQKSNSKNKSK 434
             + +  K+  K
Sbjct: 459 ATEPAQMKDSGK 456

BLAST of Tan0011229 vs. TAIR 10
Match: AT3G62080.1 (SNF7 family protein )

HSP 1 Score: 412.5 bits (1059), Expect = 4.2e-115
Identity = 225/426 (52.82%), Postives = 308/426 (72.30%), Query Frame = 0

Query: 10  VREFIREKVPDWDDEVVATARFKAFSGQKSDWEPRYLFWRDLILTIAHQFNFIFIKSSEI 69
           V+EFIR +VPDWDDEVVA ARFKAFSGQ+SDWE ++ FWRDLI+ ++ QF    I   ++
Sbjct: 6   VKEFIRREVPDWDDEVVAMARFKAFSGQRSDWELKFQFWRDLIIKVSRQFGLFIIDPVQV 65

Query: 70  RTQWFSRGGLAPLCLDHVLHLMYIEGDIIRRSDMLDPRSGQLSYLFKKLSNLMGTSKKNA 129
           +  WF RGG+ PLC+D V+ LM+ EGD++R SD+ DP SG+++ L + + NLM       
Sbjct: 66  KKAWFDRGGMTPLCIDDVVLLMHSEGDVVRISDLDDPGSGRIARLLRTVKNLMVQQPVKQ 125

Query: 130 DDLIRDDYIVLASVLKDRATEVVKCLSQSNWTSSCIVTMVKFQNLCGGPDEATAILSYLI 189
           ++++ ++ +VL  +LK++A +VVK LS+ +WTS+C+VT+ KF+NLC G +EA+A+LS+L 
Sbjct: 126 EEIL-ENTLVLVPLLKEKAADVVKILSEGHWTSTCVVTLKKFRNLCNGSNEASAVLSHLS 185

Query: 190 GCGKARYLSREKKDLLEGVKVSLSAVTVPGITTLDYDILHLIWTTEKLQQQLDVIDQRYD 249
           GCGKA  +S  + +L+EGVKVS S   +PGI+TLD DILHL+ TTEKLQ QL+V+DQR +
Sbjct: 186 GCGKAHKISINRGELIEGVKVSFSQTALPGISTLDCDILHLLRTTEKLQDQLEVMDQRCE 245

Query: 250 VSRQSALASLKSGNKKTALKHARELKITTESREKVASLLNRVEEVLNAIADAESTKTVSE 309
            S++SALASLKSG++K AL+HARELK+ TESREK  SLLNRVEEVLN IAD+ESTK VSE
Sbjct: 246 KSKKSALASLKSGHRKVALRHARELKVVTESREKCTSLLNRVEEVLNTIADSESTKMVSE 305

Query: 310 AIQIGARVMKEHEVSWDQVQHSLQELEASIDVQKQVASAIDSAPSGLILEDEDIEEEFKK 369
           AI+ GARVMK+ ++S D V   L+ELE +I+ QKQV  A++SAP   I +DEDIEEE  +
Sbjct: 306 AIKTGARVMKDIKISADDVHDYLEELEETIESQKQVEKALESAPYPDI-DDEDIEEELLE 365

Query: 370 LELEVTGQSLDASTSESGVTIATGETVATVSDDSLSTALSNLKL--VEDTGKETVNQKSN 429
           LE+++  +S       S V  AT +T      DSL+   S LKL   + T +E   + + 
Sbjct: 366 LEMDLESES-------SQVLPATSDTA-----DSLTEMFSELKLGKTKQTLEEQATEPAQ 417

Query: 430 SKNKSK 434
            K+  K
Sbjct: 426 MKDSGK 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6PBQ21.8e-1723.61Charged multivesicular body protein 7 OS=Danio rerio OX=7955 GN=chmp7 PE=2 SV=1[more]
Q871Y86.5e-0426.04Vacuolar-sorting protein snf7 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-... [more]
Match NameE-valueIdentityDescription
KAG6579154.16.5e-21188.33Charged multivesicular body protein 7, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023551290.11.1e-21088.10charged multivesicular body protein 7 [Cucurbita pepo subsp. pepo] >XP_023551291... [more]
XP_022938857.11.4e-21088.10charged multivesicular body protein 7 [Cucurbita moschata] >XP_022938858.1 charg... [more]
XP_038875996.11.2e-20988.04uncharacterized protein LOC120068336 isoform X2 [Benincasa hispida][more]
XP_038875993.18.7e-20886.50uncharacterized protein LOC120068336 isoform X1 [Benincasa hispida] >XP_03887599... [more]
Match NameE-valueIdentityDescription
A0A6J1FF907.0e-21188.10charged multivesicular body protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111444... [more]
A0A6J1K2521.2e-20787.19charged multivesicular body protein 7 OS=Cucurbita maxima OX=3661 GN=LOC11148943... [more]
A0A0A0KMY24.4e-20585.49Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G118690 PE=4 SV=1[more]
A0A6J1CX817.7e-20284.81charged multivesicular body protein 7 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A1S3CRA42.2e-19682.35charged multivesicular body protein 7 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT3G62080.22.4e-11552.31SNF7 family protein [more]
AT3G62080.14.2e-11552.82SNF7 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 284..304
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 419..440
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 419..434
NoneNo IPR availablePANTHERPTHR22761CHARGED MULTIVESICULAR BODY PROTEINcoord: 104..414
NoneNo IPR availablePANTHERPTHR22761:SF7SNF7 FAMILY PROTEINcoord: 104..414
IPR005024Snf7 familyPFAMPF03357Snf7coord: 233..380
e-value: 1.5E-14
score: 53.9

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0011229.1Tan0011229.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway
biological_process GO:0006900 vesicle budding from membrane
biological_process GO:0007034 vacuolar transport
cellular_component GO:0009898 cytoplasmic side of plasma membrane
cellular_component GO:0000815 ESCRT III complex
cellular_component GO:0005771 multivesicular body