Tan0010927 (gene) Snake gourd v1

Overview
NameTan0010927
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPectinesterase
LocationLG06: 13834761 .. 13841938 (-)
RNA-Seq ExpressionTan0010927
SyntenyTan0010927
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAACACTAAAGTTTCATTGAAGAAAAAGAATGAGCTTCTTGTCTTAGCTCTCTTTGCTACTTTACTCACAAATGCTATTGTTGTCATTTCTGAAGTCAATTATGATGAGAATAGTCATAATAATGTGGGTGTTGTAAAACACTCATGTGCCACCACTCTTTATCCACAACTATGTCATTCAACAATATCCTCTATCATGGGAGCATCCGAGTTTTTGAGTGTAAAAGATATTGTTGAAATCTCGTTGAGTGTTGCTATAGATGCAGCTAAGCATAACCATGAAAACATCAAGAAACTTTTGATGTCGCTCAGTGATCTTACTAAACGAGAGAGAATGGCTCTCCGTGATTGTATCGAGACCACCGACAAGACGCTCGACGACCTTGACAAGGTCGTAGAGGATCTTCGTGAGTATCCAAAAAAGAAATCTCTCACCCTATATGCTGATGACCTCAAAACATTTCTCAGTTCCACCATGACCAACCAGGTACTATTACTTATAAGGCAATTTTGGGATAACTTTTGAAATAAATGTGTCCATGCCTTTTTATATCTTCTGTTACAGATTTATATATATATAATAATTAATGATCTGTTGCTTATTTTAAACCAAATTATATATTTCCAGGAGACTTGTTTGGAGGGATTCTCAGGCGACGAAGCTGAAAAAAGGGTGTTGAAATTGATTGAAACCCCACATATTCACGTGACAAAGCTGTGTAGTAATGTGCTAGCCTTGATCAAGAATTTGACTGACACTGATTTAACTTTAAGGGGCTTGGTCCCTGAGCACTCTAACAAGATAGCTTCATCCTGGCATGCTGATGACTCCAAAATTGTACTCTTTTCAGACGTTACCAACGAGGTACTAATCTCTTTCTCCTCATATATTTTTAAGAGTTCAAATTTAATTCATATAATTTCAACAAAACTTAAAATTAATTAGTTTTGTTGTTATTTTAGAGATAATCTTCATCCAAATTAGTTATATAATAATAATAATAATTTTCACGGCACAAAACATACAAATGTGAACCTATTTTTAAAATTTATAGAGAAAAGACTAACATAGATTTTTTTAAAGATAATAATAAGCTAACCATATGAACCAATTTTTAACCTTTATTGAAAGTAAAATAGTAGATTTGAACATTTGAAAATATATGGATCAAAATGATATTTTAAGTTTTTTTTAACTTTCGGATACTTTAGGATCCATTTAATTTTATTCTTGAAGTTTATGTTTAAATTGAATCAAAATGAGTCTACATTGGTTAGTTAGAAGTTACTTTCTTTTTAAAAAAAAAATCTAAACTTAACAAAACAATGATAAAAAATTCCATTCAAAAAATGACACAGAGAGGTTTTCTTTTTCAAAATCAGAAGAAAATTATTAAAAGTAGGAGGGACAAAATGTAGACATTTAAAAGTATGATTAAAATAAAACAAGTCTCAAAAGTAGAGTAACCTTTTATATTCTAAAACCTTTGTTTGGTAATAATACCTTTATATTTTGTTTTAGGAAAATTATTGTTTTTGTCTCTGTTTGGATTATAAGTTAATTTGGGTCCTAAATTTAAAAGTTTACGCTTTGTTCTTGATATGAGGGTGATATTTTATTTTTGTCCCCTTAACAAAAAAAAAAAAAAATGATATGACATTTTAAATTAATTTAAATGCTTACATGATATTTTTTTTATTAAAGAAAATCTAGAAAGACCCTTGACCACCCAAAGTTATTCAAGGAGATGTGATTAACAATTTTGCACCGTTAAATATAGGAGAGATAAATTGTTAAAATACTTAAAAATTTGTATTCACTAATTATTAAGGAGTAGGCACGTTTTGAAATCTACGTCCAATGATTATTAAGAGGTATCATGGTTGAATAATTTTTTTTCCTCCTCCTTATCACCGTCGGCCTATTGTATCTGTTCTCCTTCTCCGGCACCACCAGCCACACCGTCGTACCATCTACCAAATGGTTGAAGATTTTATGGATTTTCAAACAATTTTCAGATTTGTGGTTGACAGAAACTAGGTGAAAGAGAGAGAGAGGCGGCCGCAGGGAGAGGGTGGGTGGTGTGGATGGCAGTATAAGAAAAAAAAAACCTTTTTCAAAGTTTAAAGTCTAACCGCATTGGATAGTTATTTAGATTTGATTTGATAAATATTTTATTTTTAAGAGTTAAGTATATATATACTATTTTCATGTATGAATTTACTTATTTCGTTATCTATATTTTCAAAAAACATGTAGCGTTTTGAAAATTTAAAAAAGCACAGAATCAAAAATTTGTTTTTGTTTTTAGAATTCACTTAAGAATTCAAATGCTTATTTGAAAAGAGATGAAATCATGATAGAGAAATTAAGAGAAAACAAACTTAGGGTTTGTTTTGTAGGCCATCCATGTTTTGTTTTATGTTTTCGGATTCACTGAATTCAATAAATACGTGTTTTGACATACGTGTTTTTTAAAACTGTTTTTCAGATTCTGTGATCTAAATGGTATAAATTCTTAAAACAACATTTTTATGGTTTCATTGTATCTAGATTCTAAATTTGAAATTTTAAAATTCTGAATTATATTAAACAAAGATAAAAGTCGTAATAAATATAGTATCTCATGTTTATAAACTTAATTAATTTACTTTTGTTAAATCAAAATAGGTATAATATAATATATTATAAAATATAAAATATTATATTATGAAATAATAATTATATAAAATTTTATAAAAAAAAGGTGTTCATAAATTAATTAATAATGTCTTATCTTCAACCTAATATTTAGTTTTGCAACTACAACTAATTTTATGATCTATAAATATATTATATAGAACACACTTTAAACTATTGTTATTTCTAAATTTGCTTCCCAACACATATTTGAAAACATGAAATACAACTTTGTTTTCATTAAATCCTTTGTTTTCAAATTTATGTTTTCGGATTGTCTACCAAACAGACCCTTAATTTTCAAAAACCAAAAACGAAGGTCCGTTGGATAACCAATTTGGTTTTTGAAAATTAAGCCTATAAATGCTAACTTTCTACCAATGAGTTTCTATATTTTCTTATCTAGTTTGTACCTATGTTTTAAAAAACAAATCTAAGTTTTGAATACTAAAAAAGTTAGTGTTCAAAAACATATTTTTATTTTTAAAATTTGGCTAGAGACATAATGGTTAATCAAGAAACATGGAAAGCATTGTAGAGAATATGGAGAAAATTTGTGGAAAAACAACCATAATTTTCAAAAAACCAAATGATTATCATGGGCCTAAATGATTATTAAACGAGCCCACTTAGGCCCCGTTTGATAACCATTTTGTTTTTTGTTTTTTGTTTTTGAAAGTTAAGCTTATGTTCACCTAATTTCTCTACAATAGAGATCATATCCCTTAAGTAAACATTTGAACTCTTAGTCAAATTCTAAAAACAAAGTCAAGTTTTTGAAAGCTACTTTTTTTATTTTTCAAAAGTTGGCTTGGTTTTAAAAACATAGGTACAAAGTAGATAAGAAAACATATAAACTCATTGGTATAAGTAGTATTTTTAGGTTTAAATTTCAAAAACTAAAAACAAAAATCAAAATCGTTATCAAACGGGGCCTTAGTTTTTAATTTTTAGTTTTTGAAAATTAAGTCTATACATATTACTTTCACCTATGAATTTCTTTGCGTATATTTTTTAAAACTAACCCAAATTTTGAAAATAAAAAAAGTATTAGTTCTTAAAAACTTGCTTTTGTTTTTTAAATTTGGCTAAGAATTCAAATGTTTTTTTAAGAAATATGAAAGTCATTTTAGAAAATTGTAGAGAAATGATGAGATAATAATTCAATTGTTTTCTTAGGAATTATAAGAGCTAATGTAGACAATTATAGGAAAATAATGATGTGAAAGAATGTATAATTTTCGAAAACCAAAAACCAAAAACCAAATGATTATGTGATAGAGCCTAAAGTATAAGATTTTAAAATATAGGAACTAAAAGAAGCTATTACCATGAATATGGGGACCAACTGAAGTTGTTCCCAAAATCTCATAGACCAAAAAAAATATTTTTTTCTGCTTTTGTTTTGATTTAAATTTTAGTAGACCAGAAAAATAAAATATAGGTGGTTAATTTTATTTTTTTTTTTCATTTCTGAAATTTAACAAGGACTTCAAGAATTTTTTTAATTTACATTTGTGCCAGCGAAAAATTTATTAATATCATAAAAGCCAATTTATTATAATTAGGCATCATTGGTTGATAACAAATATTTGTATTAAAAGAATAAATTTATTATTATTTATTATCACAATAATAAGATTTAATAATAATTTATATTAATGATTGCAGTTCTAATAAAAATAATATAAATATTTGTAGTTCAAGTATTTTTATTTTAATTAATTACTGTAGGCTAACCAAATAAATGGTCAAAATAAAACATTTTTCTATCAAAGAAAATAGTAATAATTTTTAATTAAAAAAAGAGAGAAATTTTATTATAGATTATTGTTATATTAATTAAAAATGAGACTAGCATACTAAGTTGATGAATTTTTAAATTAGTTAGAAATTTGAATGGATATTTTACCAATTATTATATGTCTCATCCCCTATTTTAATTCTCGACTGTTTTTAGTAAACAAAATCAACATATCAGTAAACATCAAAGGAATAATACTTTCATTCCATATTAATCTTCATCCTAATTTCCCATCGTTTAAATTCTCATTTTGAATAACAAAAAGCAAATTTTAAATAGGACAAAAGTAATTTTAGTAGGCTATTTTAATTTGGATTTTGAATTATAACAGGAGGAAGGTGAGAATAGGGGCAGGGAGGGTTTAGAAGATGGAATCAAGTGGCCAAAATGGATGTCAATTAGCGACGAGAGGCTTTTGGAGTCATCGTCAGAAGCGGCGGCGGCGGACGTGGTGGTGGCGGCGGACGGAAGTGGAAATTTTGGAACAGTGTCGGAGGCAGTGGCGGCGGCGCCGAATAAGAGCAGCAAAAGATATGTAATAAAAATAAAGGCCGGAGTTTACAAGGAAAATGTTGAAGTCCCAAGAAGTAAAAAGAATATTATGTTTTGGGGAGATGGAAGATCCAATACCATTATAACAGGTAGCAGAAGTTTTGGGACTGGCTGGACAACCTTCAATTCCGCCACAGTCGGTAAACCAAAGCAACTAACTTACTTTTAAACTAGTGCTTTATTACAAACCACTTTAAAATGAAGTGAAGTTATCCATATTATTACATTGTTATTGACATGTTGACATAACAATATGAATATGACGTCGATAAAAGAGGACCTTATAAAATTGATCCAATATTAAAGAAATGATAACAATAGCTAACATAAATGTGAATATAATATTATAGAAGTATTTTAATGTTTATTTTCTAATTTATATATATTCTTAATGTTGTTAAAAAAAATTTAGAAAAATATCTATCATTTTATAGATATTTTCATCGGCATTTCCATAAAACTGAGACTTCGACATTACCGTTGGCATTTTAAATCTGGGTTATTAATAGGTTAAAACAAAATTATTATCATTTAAAAAAAATTAACTAAAGATTATCCATTTTTAAAAAATACAAAATGTTTTTTTTGTGAAAAAGTGATTTTATACCATGTTCTTCTTTGTAAGTATTTCTGGAACAAAATAGTTAATTAGATTTTTTTTGTTTCGTCCTAAACTTGTATAACTTTTAAAATTGTGTTTAATAGTTTTCGAAGGTTACAAAATATCTAAACATAGGTTCTTGATCCTTCAAAATATTCTTTTAATAGACTGTTTAACTATATTAAAAGTATCTAATTAAGTTACTAAGTAGTTAATTTTGTGTCTAATACATTTCAAAAAGTTTATAAATTGTCTCCTAAATTAATAATTTATTAAATATAAAATTAAAAGTTCAAAGATTTATTGAATAGTTTTTAAAATTAAAGGAGTGTTAATTATTAGACTCATATAAAAGTTGAGATTTATTAGATACTTTCTAAAATTCAAAGATGTAAACTAGGATTCTCATAATTTCACTTTATTTATTTTTTAAGATTTCTCAAACCATTTTTTATTATTGAAAAATTTAAAACTTTTAAATGGTTAAAAACAATTTTGTCCTTCTCTAAAATAAATTTTAACAATGGGCTCCTTTCTACAAATTATTCAATTGCTATAATGTGGTTGATTCATAATAGTAGGTGGTGAGAATTCAATAATTTTCATTGAATTTGCACCCAAATGTTGTGAACTCATTTTTCTAACACAATATTTTTCTCTTGAAAAAAATTAATTTTTTTTAATGTGATTACCACGTGGATTGCAAAATACATAGGAACGGACTCAAGTTACACCCAAATATTTTCCATTTTCATGCCCCCCTTTGTAAAGTTTCCAAAAGTGCTTTAGTTTTAGTCATTTAAAAACACTTTAAAAATATATGGCCAACAACAAACACTTATTTTGTTGGCAGCTGCGGTGGGGGATGGATTCTTAGCTCGAGATATAACTATTGAAAACACAGCTGGAGCAGCCAATCACCAAGCCGTAGCTCTCCGCGTTGGATCAGATCACTCAGCCTTCTATCGTTGTGGCATTCAAGCCTACCAAGACACACTCTACGTTCACTCCAACCGCCAATTCTTCGTGAACTGTGTAATTGCAGGCACAATCGACTTCATATTCGGAAACGCAGCAGTAGTAATTCAAGACTCAGTGCTCACCCCTCGTCGACCAGACCCTAACCAACAGAACATGGTAACAGCACAAGGAAGAATCGAGGCCAATCAAAACACGGGAATTGTGATCCAAAAGTGCACGATTAAAGCAACCTCGGACTTAGAGCCAGTGATAAAAGAATTCCCAAGTTTCCTTGGTAGACCATGGAAGGAATATGCTAGGGTTGTGGTTATGCAAACAGATATTACCAATGTGATTGACCCAAAAGGGTGGGCGCCATGGAGTGGTGATTTTGCTCTTAAGACACTCTTCTTTGCAGAATATGACAACAAGGGAGATGGAGCCAATACATGTTGTAGGGTGGAGTGGTCCTCGCTTATTAATGCTGATCAAGCTAAGGCTTTTACGGCTGGACAATTCATTAGTGGTGCCGATTGGCTCCCATCCACTGCCTTCCCCTATCAGCTTGGCCTCTAA

mRNA sequence

ATGGCAAACACTAAAGTTTCATTGAAGAAAAAGAATGAGCTTCTTGTCTTAGCTCTCTTTGCTACTTTACTCACAAATGCTATTGTTGTCATTTCTGAAGTCAATTATGATGAGAATAGTCATAATAATGTGGGTGTTGTAAAACACTCATGTGCCACCACTCTTTATCCACAACTATGTCATTCAACAATATCCTCTATCATGGGAGCATCCGAGTTTTTGAGTGTAAAAGATATTGTTGAAATCTCGTTGAGTGTTGCTATAGATGCAGCTAAGCATAACCATGAAAACATCAAGAAACTTTTGATGTCGCTCAGTGATCTTACTAAACGAGAGAGAATGGCTCTCCGTGATTGTATCGAGACCACCGACAAGACGCTCGACGACCTTGACAAGGTCGTAGAGGATCTTCGTGAGTATCCAAAAAAGAAATCTCTCACCCTATATGCTGATGACCTCAAAACATTTCTCAGTTCCACCATGACCAACCAGGAGACTTGTTTGGAGGGATTCTCAGGCGACGAAGCTGAAAAAAGGGTGTTGAAATTGATTGAAACCCCACATATTCACGTGACAAAGCTGTGTAGTAATGTGCTAGCCTTGATCAAGAATTTGACTGACACTGATTTAACTTTAAGGGGCTTGGTCCCTGAGCACTCTAACAAGATAGCTTCATCCTGGCATGCTGATGACTCCAAAATTGTACTCTTTTCAGACGTTACCAACGAGGAGGAAGGTGAGAATAGGGGCAGGGAGGGTTTAGAAGATGGAATCAAGTGGCCAAAATGGATGTCAATTAGCGACGAGAGGCTTTTGGAGTCATCGTCAGAAGCGGCGGCGGCGGACGTGGTGGTGGCGGCGGACGGAAGTGGAAATTTTGGAACAGTGTCGGAGGCAGTGGCGGCGGCGCCGAATAAGAGCAGCAAAAGATATGTAATAAAAATAAAGGCCGGAGTTTACAAGGAAAATGTTGAAGTCCCAAGAAGTAAAAAGAATATTATGTTTTGGGGAGATGGAAGATCCAATACCATTATAACAGGTAGCAGAAGTTTTGGGACTGGCTGGACAACCTTCAATTCCGCCACAGTCGCTGCGGTGGGGGATGGATTCTTAGCTCGAGATATAACTATTGAAAACACAGCTGGAGCAGCCAATCACCAAGCCGTAGCTCTCCGCGTTGGATCAGATCACTCAGCCTTCTATCGTTGTGGCATTCAAGCCTACCAAGACACACTCTACGTTCACTCCAACCGCCAATTCTTCGTGAACTGTGTAATTGCAGGCACAATCGACTTCATATTCGGAAACGCAGCAGTAGTAATTCAAGACTCAGTGCTCACCCCTCGTCGACCAGACCCTAACCAACAGAACATGGTAACAGCACAAGGAAGAATCGAGGCCAATCAAAACACGGGAATTGTGATCCAAAAGTGCACGATTAAAGCAACCTCGGACTTAGAGCCAGTGATAAAAGAATTCCCAAGTTTCCTTGGTAGACCATGGAAGGAATATGCTAGGGTTGTGGTTATGCAAACAGATATTACCAATGTGATTGACCCAAAAGGGTGGGCGCCATGGAGTGGTGATTTTGCTCTTAAGACACTCTTCTTTGCAGAATATGACAACAAGGGAGATGGAGCCAATACATGTTGTAGGGTGGAGTGGTCCTCGCTTATTAATGCTGATCAAGCTAAGGCTTTTACGGCTGGACAATTCATTAGTGGTGCCGATTGGCTCCCATCCACTGCCTTCCCCTATCAGCTTGGCCTCTAA

Coding sequence (CDS)

ATGGCAAACACTAAAGTTTCATTGAAGAAAAAGAATGAGCTTCTTGTCTTAGCTCTCTTTGCTACTTTACTCACAAATGCTATTGTTGTCATTTCTGAAGTCAATTATGATGAGAATAGTCATAATAATGTGGGTGTTGTAAAACACTCATGTGCCACCACTCTTTATCCACAACTATGTCATTCAACAATATCCTCTATCATGGGAGCATCCGAGTTTTTGAGTGTAAAAGATATTGTTGAAATCTCGTTGAGTGTTGCTATAGATGCAGCTAAGCATAACCATGAAAACATCAAGAAACTTTTGATGTCGCTCAGTGATCTTACTAAACGAGAGAGAATGGCTCTCCGTGATTGTATCGAGACCACCGACAAGACGCTCGACGACCTTGACAAGGTCGTAGAGGATCTTCGTGAGTATCCAAAAAAGAAATCTCTCACCCTATATGCTGATGACCTCAAAACATTTCTCAGTTCCACCATGACCAACCAGGAGACTTGTTTGGAGGGATTCTCAGGCGACGAAGCTGAAAAAAGGGTGTTGAAATTGATTGAAACCCCACATATTCACGTGACAAAGCTGTGTAGTAATGTGCTAGCCTTGATCAAGAATTTGACTGACACTGATTTAACTTTAAGGGGCTTGGTCCCTGAGCACTCTAACAAGATAGCTTCATCCTGGCATGCTGATGACTCCAAAATTGTACTCTTTTCAGACGTTACCAACGAGGAGGAAGGTGAGAATAGGGGCAGGGAGGGTTTAGAAGATGGAATCAAGTGGCCAAAATGGATGTCAATTAGCGACGAGAGGCTTTTGGAGTCATCGTCAGAAGCGGCGGCGGCGGACGTGGTGGTGGCGGCGGACGGAAGTGGAAATTTTGGAACAGTGTCGGAGGCAGTGGCGGCGGCGCCGAATAAGAGCAGCAAAAGATATGTAATAAAAATAAAGGCCGGAGTTTACAAGGAAAATGTTGAAGTCCCAAGAAGTAAAAAGAATATTATGTTTTGGGGAGATGGAAGATCCAATACCATTATAACAGGTAGCAGAAGTTTTGGGACTGGCTGGACAACCTTCAATTCCGCCACAGTCGCTGCGGTGGGGGATGGATTCTTAGCTCGAGATATAACTATTGAAAACACAGCTGGAGCAGCCAATCACCAAGCCGTAGCTCTCCGCGTTGGATCAGATCACTCAGCCTTCTATCGTTGTGGCATTCAAGCCTACCAAGACACACTCTACGTTCACTCCAACCGCCAATTCTTCGTGAACTGTGTAATTGCAGGCACAATCGACTTCATATTCGGAAACGCAGCAGTAGTAATTCAAGACTCAGTGCTCACCCCTCGTCGACCAGACCCTAACCAACAGAACATGGTAACAGCACAAGGAAGAATCGAGGCCAATCAAAACACGGGAATTGTGATCCAAAAGTGCACGATTAAAGCAACCTCGGACTTAGAGCCAGTGATAAAAGAATTCCCAAGTTTCCTTGGTAGACCATGGAAGGAATATGCTAGGGTTGTGGTTATGCAAACAGATATTACCAATGTGATTGACCCAAAAGGGTGGGCGCCATGGAGTGGTGATTTTGCTCTTAAGACACTCTTCTTTGCAGAATATGACAACAAGGGAGATGGAGCCAATACATGTTGTAGGGTGGAGTGGTCCTCGCTTATTAATGCTGATCAAGCTAAGGCTTTTACGGCTGGACAATTCATTAGTGGTGCCGATTGGCTCCCATCCACTGCCTTCCCCTATCAGCTTGGCCTCTAA

Protein sequence

MANTKVSLKKKNELLVLALFATLLTNAIVVISEVNYDENSHNNVGVVKHSCATTLYPQLCHSTISSIMGASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRDCIETTDKTLDDLDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEKRVLKLIETPHIHVTKLCSNVLALIKNLTDTDLTLRGLVPEHSNKIASSWHADDSKIVLFSDVTNEEEGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSEAVAAAPNKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTFNSATVAAVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKCTIKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDGANTCCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL
Homology
BLAST of Tan0010927 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 3.5e-170
Identity = 327/595 (54.96%), Postives = 422/595 (70.92%), Query Frame = 0

Query: 7   SLKKKNELLVLALFATLLTNAIV--VISEVNYDENSHNN-----VGVVKHSCATTLYPQL 66
           ++ KK + L LALFATLL  A V  +++ VN  +NS +N       ++K SC++T YP L
Sbjct: 22  NIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDL 81

Query: 67  CHSTISSIMGAS-EFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRD 126
           C S I+++  AS +  S KD++E+SL++   A +HN+  I+KLL   ++LTKRE++AL D
Sbjct: 82  CFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLL-KRTNLTKREKVALHD 141

Query: 127 CIETTDKTLDDLDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEK 186
           C+ET D+TLD+L K VEDL EYP KKSL+ +ADDLKT +S+ MTNQ TCL+GFS D+A K
Sbjct: 142 CLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANK 201

Query: 187 RVLKLIETPHIHVTKLCSNVLALIKNLTDTDLTLRGLVPEHSNKIASSWHADDSKIVLFS 246
            V   +    +HV K+CSN LA+IKN+TDTD+    ++   SN         + K++   
Sbjct: 202 HVRDALSDGQVHVEKMCSNALAMIKNMTDTDM----MIMRTSN---------NRKLI--- 261

Query: 247 DVTNEEEGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSE 306
           + T+  +G             WP W+S  D RLL+SSS     +VVVAADGSGNF TV+ 
Sbjct: 262 EETSTVDG-------------WPAWLSTGDRRLLQSSS--VTPNVVVAADGSGNFKTVAA 321

Query: 307 AVAAAPNKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTF 366
           +VAAAP   +KRY+I+IKAGVY+ENVEV +  KNIMF GDGR+ TIITGSR+   G TTF
Sbjct: 322 SVAAAPQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTF 381

Query: 367 NSATVAAVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNR 426
            SATVA VG+GFLARDIT +NTAG + HQAVALRVG+D SAFY C + AYQDTLYVHSNR
Sbjct: 382 KSATVAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNR 441

Query: 427 QFFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKC 486
           QFFVNC+IAGT+DFIFGNAA V+Q+  +  R+P+  Q+NMVTAQGR + NQNTGIVIQK 
Sbjct: 442 QFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKS 501

Query: 487 TIKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFA 546
            I ATSDL+PV   FP++LGRPWKEY+R V+MQ+ IT+VI P GW  W G+FAL TLF+ 
Sbjct: 502 RIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYG 561

Query: 547 EYDNKGDGANTCCRVEWSS---LINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
           E+ N G GA T  RV+W     + +A +A+AFT G FI+G+ WL ST FP+ LGL
Sbjct: 562 EHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Tan0010927 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 6.5e-169
Identity = 323/595 (54.29%), Postives = 419/595 (70.42%), Query Frame = 0

Query: 7   SLKKKNELLVLALFATLLTNAIV--VISEVNYDENSHNN-----VGVVKHSCATTLYPQL 66
           ++ KK + L LALFATLL  A V  +++ VN  +NS +N       ++K SC++T YP L
Sbjct: 22  NIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDL 81

Query: 67  CHSTISSIMGAS-EFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRD 126
           C S I+++  AS +  S KD++E+SL++   A +HN+  I+KLL   ++LTKRE++AL D
Sbjct: 82  CFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLL-KRTNLTKREKVALHD 141

Query: 127 CIETTDKTLDDLDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEK 186
           C+ET D+TLD+L K VEDL EYP KKSL+ +ADDLKT +S+ MTNQ TCL+GFS D+A K
Sbjct: 142 CLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANK 201

Query: 187 RVLKLIETPHIHVTKLCSNVLALIKNLTDTDLTLRGLVPEHSNKIASSWHADDSKIVLFS 246
            V   +    +HV K+CSN LA+IKN+TDTD+ +  +   ++ K+              +
Sbjct: 202 HVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMI--MRTSNNRKL--------------T 261

Query: 247 DVTNEEEGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSE 306
           + T+  +G             WP W+S  D RLL+SSS     + VVAADGSGNF TV+ 
Sbjct: 262 EETSTVDG-------------WPAWLSPGDRRLLQSSS--VTPNAVVAADGSGNFKTVAA 321

Query: 307 AVAAAPNKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTF 366
           AVAAAP   +KRY+I+IKAGVY+ENVEV +  KNIMF GDGR+ TIITGSR+   G TTF
Sbjct: 322 AVAAAPQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTF 381

Query: 367 NSATVAAVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNR 426
            SAT A VG+GFLARDIT +NTAG + HQAVALRVG+D SAFY C + AYQDTLYVHSNR
Sbjct: 382 KSATAAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNR 441

Query: 427 QFFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKC 486
           QFFVNC+IAGT+DFIFGNAA V+Q+  +  R+P+  Q+NMVTAQGR + NQNTGIVIQK 
Sbjct: 442 QFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKS 501

Query: 487 TIKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFA 546
            I ATSDL+PV   FP++LGRPWKEY+R V+MQ+ IT++I P GW  W G+FAL TLF+ 
Sbjct: 502 RIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYG 561

Query: 547 EYDNKGDGANTCCRVEWSS---LINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
           E+ N G GA T  RV+W     + +A +A+AFT G FI+G+ WL ST FP+ LGL
Sbjct: 562 EHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Tan0010927 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 1.2e-165
Identity = 314/598 (52.51%), Postives = 413/598 (69.06%), Query Frame = 0

Query: 9   KKKNELLVLALFATLLTNAI--VVISEVNYDENSHNNV----------GVVKHSCATTLY 68
           K+K ++ +  + + LL  A+  VV    ++ +NS ++            +VK +C+ TL+
Sbjct: 16  KRKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLH 75

Query: 69  PQLCHSTISSIMGAS-EFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMA 128
           P+LC+S I ++   S +  S KD++E+SL++ + A + N+  +K+L+ +   LT RE++A
Sbjct: 76  PELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVA 135

Query: 129 LRDCIETTDKTLDDLDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDE 188
           L DC+ET D+TLD+L   VEDL  YP KKSL  + +DLKT +SS +TNQETCL+GFS DE
Sbjct: 136 LHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDE 195

Query: 189 AEKRVLKLIETPHIHVTKLCSNVLALIKNLTDTDLTLRGLVPEHSNKIASSWHADDSKIV 248
           A+K+V K++     HV K+CSN LA+I N+TDTD+         +N++  S  A++ K+V
Sbjct: 196 ADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDI---------ANEMKLSAPANNRKLV 255

Query: 249 LFSDVTNEEEGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGT 308
                              ED  +WP+W+S  D RLL+SS+     DVVVAADGSG++ T
Sbjct: 256 -------------------EDNGEWPEWLSAGDRRLLQSST--VTPDVVVAADGSGDYKT 315

Query: 309 VSEAVAAAPNKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGW 368
           VSEAV  AP KSSKRYVI+IKAGVY+ENV+VP+ K NIMF GDG+SNTIIT SR+   G 
Sbjct: 316 VSEAVRKAPEKSSKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGS 375

Query: 369 TTFNSATVAAVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVH 428
           TTF+SATV  V    LARDIT +NTAGA+ HQAVAL VGSD SAFYRC + AYQDTLYVH
Sbjct: 376 TTFHSATVVRVAGKVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVH 435

Query: 429 SNRQFFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVI 488
           SNRQFFV C++AGT+DFIFGN A V QD  +  RRP   Q+NMVTAQGR + NQNTGIVI
Sbjct: 436 SNRQFFVQCLVAGTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVI 495

Query: 489 QKCTIKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTL 548
           QKC I ATSDL PV K FP++LGRPWKEY+R V+MQ+ IT+VI P GW  W+G+FAL TL
Sbjct: 496 QKCRIGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTL 555

Query: 549 FFAEYDNKGDGANTCCRVEWSS---LINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
           F+ EY N G GA T  RV+W     + ++ +A+A+T G+FI+G  WL ST FP+ LGL
Sbjct: 556 FYGEYANTGAGAPTSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

BLAST of Tan0010927 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 583.2 bits (1502), Expect = 3.4e-165
Identity = 319/594 (53.70%), Postives = 408/594 (68.69%), Query Frame = 0

Query: 10  KKNELLVL--ALFATLLTNAIVVISEVNYDEN-----SHNNVGVVKHSCATTLYPQLCHS 69
           KKN+ LVL  A  A L   A+  IS      N     S ++  V++ SC++T YP+LC S
Sbjct: 15  KKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCIS 74

Query: 70  TISSIMGASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRDCIET 129
            + +  G  E  S KD++E S+++ I A +HN+  +KKL+     LT RE+ AL DC+ET
Sbjct: 75  AVVT-AGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLET 134

Query: 130 TDKTLDDLDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEKRVLK 189
            D+TLD+L + VEDL  YP KK+L  +A DLKT +SS +TNQETCL+GFS D+A+K+V K
Sbjct: 135 IDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRK 194

Query: 190 LIETPHIHVTKLCSNVLALIKNLTDTDLT---LRGLVPEHSNKIASSWHADDSKIVLFSD 249
            +    IHV  +CSN LA+IKN+TDTD+     +  +  ++ K+      ++ +  +  D
Sbjct: 195 ALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKE----ENQETTVAVD 254

Query: 250 VTNEEEGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSEA 309
           +    E ++ G         WP W+S  D RLL+ S     AD  VAADGSG F TV+ A
Sbjct: 255 IAGAGELDSEG---------WPTWLSAGDRRLLQGS--GVKADATVAADGSGTFKTVAAA 314

Query: 310 VAAAPNKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTFN 369
           VAAAP  S+KRYVI IKAGVY+ENVEV + KKNIMF GDGR+ TIITGSR+   G TTF+
Sbjct: 315 VAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFH 374

Query: 370 SATVAAVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQ 429
           SATVAAVG+ FLARDIT +NTAG + HQAVALRVGSD SAFY C + AYQDTLYVHSNRQ
Sbjct: 375 SATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQ 434

Query: 430 FFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKCT 489
           FFV C+IAGT+DFIFGNAAVV+QD  +  RRP+  Q+NMVTAQGR + NQNTGIVIQKC 
Sbjct: 435 FFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCR 494

Query: 490 IKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAE 549
           I ATSDL+ V   FP++LGRPWKEY++ V+MQ+ I++VI P+GW+ W+G FAL TL + E
Sbjct: 495 IGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYRE 554

Query: 550 YDNKGDGANTCCRVEWSS---LINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
           Y N G GA T  RV+W     +  A +A+ +TAGQFI G  WL ST FP+ LGL
Sbjct: 555 YSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Tan0010927 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 576.2 bits (1484), Expect = 4.1e-163
Identity = 321/600 (53.50%), Postives = 407/600 (67.83%), Query Frame = 0

Query: 4   TKVSLKKKNELLVL--ALFATLLTNAIVVISEVNYDENSHNNV--------GVVKHSCAT 63
           +K S  K N+ L+L  A  A LL  +IV I+    ++N +  +         ++K  C++
Sbjct: 9   SKFSDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSS 68

Query: 64  TLYPQLCHSTISSIMGASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRER 123
           TLYP+LC S +++  G  E  S K+++E SL++   A KHN+  +KKL+     LT RE 
Sbjct: 69  TLYPELCFSAVAA-TGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREV 128

Query: 124 MALRDCIETTDKTLDDLDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSG 183
            AL DC+ET D+TLD+L   VEDL +YPK+KSL  +ADDLKT +SS +TNQ TCL+GFS 
Sbjct: 129 TALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSY 188

Query: 184 DEAEKRVLKLIETPHIHVTKLCSNVLALIKNLTDTDLTLRGLVPEHSNKIASSWHADDSK 243
           D+A+++V K +    +HV  +CSN LA+IKN+T+TD             IA+    D S 
Sbjct: 189 DDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETD-------------IANFELRDKS- 248

Query: 244 IVLFSDVTNEEEGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNF 303
               S  TN    + +   G  D   WPKW+S+ D RLL+ S+    AD  VA DGSG+F
Sbjct: 249 ----STFTNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGST--IKADATVADDGSGDF 308

Query: 304 GTVSEAVAAAPNKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGT 363
            TV+ AVAAAP KS+KR+VI IKAGVY+ENVEV + K NIMF GDGR  TIITGSR+   
Sbjct: 309 TTVAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVD 368

Query: 364 GWTTFNSATVAAVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLY 423
           G TTF+SATVAAVG+ FLARDIT +NTAG + HQAVALRVGSD SAFY+C + AYQDTLY
Sbjct: 369 GSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLY 428

Query: 424 VHSNRQFFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGI 483
           VHSNRQFFV C I GT+DFIFGNAA V+QD  +  RRP+  Q+NMVTAQGR + NQNTGI
Sbjct: 429 VHSNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGI 488

Query: 484 VIQKCTIKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALK 543
           VIQ C I  TSDL  V   FP++LGRPWKEY+R V+MQ+DI++VI P+GW  WSG FAL 
Sbjct: 489 VIQNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALD 548

Query: 544 TLFFAEYDNKGDGANTCCRVEWS--SLINAD-QAKAFTAGQFISGADWLPSTAFPYQLGL 591
           TL + EY N+G GA T  RV+W    +I +D +A+ FTAGQFI G  WL ST FP+ L L
Sbjct: 549 TLTYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Tan0010927 vs. NCBI nr
Match: XP_008458040.2 (PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo])

HSP 1 Score: 776.5 bits (2004), Expect = 1.6e-220
Identity = 409/586 (69.80%), Postives = 476/586 (81.23%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEVNYDENSHNNVGVVKHSCATTLYPQLCHSTISSIMG 70
           +N  L LALFAT LLTNAI+V +  N +    N   +++ SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRDCIETTDKTLDD 130
            S  LS+KDIV++SLSVA+DAAK N+ NIK  LMS ++++KR+R+ L DC+++TD+T+ +
Sbjct: 62  TSNLLSLKDIVQLSLSVAMDAAKLNNGNIKN-LMSFNNVSKRDRIPLHDCVKSTDRTIYE 121

Query: 131 LDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEKRVLKLIETPHI 190
           LDK +ED REYP KKSLT YADDLKTFLSS +TNQ TCL+GF  D+ EK VL++IE  HI
Sbjct: 122 LDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTHI 181

Query: 191 HVTKLCSNVLALIKNLTDTDLTL----RGLVPEHSNKIAS-SWHADDSKIVLFSDVTNEE 250
           H TKLCSN LAL+K LT T++ L      +V +   KI S S   +D KIVLFS   N+E
Sbjct: 182 HATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFS---NQE 241

Query: 251 EGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSEAVAAAP 310
           EGEN  RE LE+GIKWP+WMSI D++LLESSS  AAADVVVA DGSGN+ TVSEAV AAP
Sbjct: 242 EGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAP 301

Query: 311 NKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTFNSATVA 370
           +K+SKRY+IKIKAGVY+ENV+VP SK+NIMFWGDGRSNTIIT  RS G+GW+TFNSATV 
Sbjct: 302 SKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVV 361

Query: 371 AVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNC 430
           AVGDGFLARDI+ ENTAG AN QAVALRVGSDHSAFYRCG+  YQDTLYVHSNRQFFVNC
Sbjct: 362 AVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNC 421

Query: 431 VIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKCTIKATS 490
           V+AGT+DF+FGNAA VIQ+S LTPR+P PNQ+NMVTAQ R + NQNTGIVIQKC IKATS
Sbjct: 422 VVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATS 481

Query: 491 DLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKG 550
           DLEPVIKEFPSFLGRPW+EYARVVVMQT ITNVID KGW+ W+GD  +K  +FAEYDN G
Sbjct: 482 DLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNG 541

Query: 551 DGANTCCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
            GANT  RV WS +IN   AK FTA +FI GADWLPST FPYQLGL
Sbjct: 542 AGANTSGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 580

BLAST of Tan0010927 vs. NCBI nr
Match: TYK30782.1 (pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 775.8 bits (2002), Expect = 2.7e-220
Identity = 404/581 (69.54%), Postives = 471/581 (81.07%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEVNYDENSHNNVGVVKHSCATTLYPQLCHSTISSIMG 70
           +N  L LALFAT LLTNAI+V +  N +    N   +++ SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRDCIETTDKTLDD 130
            S  LS+KDIV++SLSVA+DAAK N+ NIKKL MS+++++KR+R+ L DC+++TD+T+ +
Sbjct: 62  TSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIYE 121

Query: 131 LDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEKRVLKLIETPHI 190
           LDK +ED REYP KKSLT YADDLKTFLSS +TNQ TCL+GF  D+ EKRVL++IE  HI
Sbjct: 122 LDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTHI 181

Query: 191 HVTKLCSNVLALIKNLTDTDLTLRGLVPEHSNKIASSWHADDSKIVLFSDVTNEEEGENR 250
           H TKLCSN LAL+K LT    T   L+ E + K +    +     VLFS   N+EEGEN 
Sbjct: 182 HATKLCSNALALVKKLT----TEIALIDEKNLKKSRKARSH----VLFS---NQEEGENG 241

Query: 251 GREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSEAVAAAPNKSSK 310
            RE LE+GIKWP+WMSI D++LLESSS  AA DVVVA DGSGN+ TVSEAV AAP+K+SK
Sbjct: 242 RREDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKNSK 301

Query: 311 RYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTFNSATVAAVGDG 370
           RY+IKIKAGVY+ENV+VP SK+NIMFWGDGRSNTIIT  RS G+GW+TFNSATV AVGDG
Sbjct: 302 RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDG 361

Query: 371 FLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVIAGT 430
           FLARDI+ ENTAG AN QAVALRVGSDHSAFYRCG+  YQDTLYVHSNRQFFVNCV+AGT
Sbjct: 362 FLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGT 421

Query: 431 IDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKCTIKATSDLEPV 490
           +DF+FGNAA VIQ+S LTPR+P PNQ+NMVTAQ R + NQNTGIVIQKC IKATSDLEPV
Sbjct: 422 VDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPV 481

Query: 491 IKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDGANT 550
           IKEFPSFLGRPW+EYARVVVMQT ITNVID KGW+ W+GD  +K  +FAEYDN G GANT
Sbjct: 482 IKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNGAGANT 541

Query: 551 CCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
             RV WS +IN   AK FTA +FI GADWLPST FPYQLGL
Sbjct: 542 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 569

BLAST of Tan0010927 vs. NCBI nr
Match: KAA0026177.1 (pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 770.0 bits (1987), Expect = 1.5e-218
Identity = 398/581 (68.50%), Postives = 465/581 (80.03%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEVNYDENSHNNVGVVKHSCATTLYPQLCHSTISSIMG 70
           +N  L LALFAT LLTNAI+V +  N +    N   +++ SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRDCIETTDKTLDD 130
            S  LS+KDIV++SLSVA+DAAK N+ NIKKL MS+++++KR+R+ L DC+++TD+T+ +
Sbjct: 62  TSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIYE 121

Query: 131 LDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEKRVLKLIETPHI 190
           LDK +ED REYP KKSLT YADDLKTFLSS +TNQ TCL+GF  D+ EKRVL++IE  HI
Sbjct: 122 LDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTHI 181

Query: 191 HVTKLCSNVLALIKNLTDTDLTLRGLVPEHSNKIASSWHADDSKIVLFSDVTNEEEGENR 250
           H TKLCSN LAL+K LT T++ L                 D+  +       + EEGEN 
Sbjct: 182 HATKLCSNALALVKKLT-TEIAL----------------IDEKNLKKSRKARSHEEGENG 241

Query: 251 GREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSEAVAAAPNKSSK 310
            RE LE+GIKWP+WMSI D++LLESSS  AA DVVVA DGSGN+ TVSEAV AAP+K+SK
Sbjct: 242 RREDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKNSK 301

Query: 311 RYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTFNSATVAAVGDG 370
           RY+IKIKAGVY+ENV+VP SK+NIMFWGDGRSNTIIT  RS G+GW+TFNSATV AVGDG
Sbjct: 302 RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDG 361

Query: 371 FLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVIAGT 430
           FLARDI+ ENTAG AN QAVALRVGSDHSAFYRCG+  YQDTLYVHSNRQFFVNCV+AGT
Sbjct: 362 FLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGT 421

Query: 431 IDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKCTIKATSDLEPV 490
           +DF+FGNAA VIQ+S LTPR+P PNQ+NMVTAQ R + NQNTGIVIQKC IKATSDLEPV
Sbjct: 422 VDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPV 481

Query: 491 IKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDGANT 550
           IKEFPSFLGRPW+EYARVVVMQT ITNVID KGW+ W+GD  +K  +FAEYDN G GANT
Sbjct: 482 IKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNGAGANT 541

Query: 551 CCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
             RV WS +IN   AK FTA +FI GADWLPST FPYQLGL
Sbjct: 542 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 563

BLAST of Tan0010927 vs. NCBI nr
Match: XP_011659194.1 (pectinesterase [Cucumis sativus] >KGN44619.1 hypothetical protein Csa_015815 [Cucumis sativus])

HSP 1 Score: 766.5 bits (1978), Expect = 1.6e-217
Identity = 409/586 (69.80%), Postives = 475/586 (81.06%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEVNYDENSHNNVGVVKHSCATTLYPQLCHSTISSIMG 70
           +N  L LALFAT LLTNAI+V +  N +   +N + V++ SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMNNILSVIETSCAITLYPQLCHSTISSILG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRDCIETTDKTLDD 130
            S  LS+KDIVE+SLSVA+DAAK N+ENIKKL MS + + KR+R+AL DC++TTD+T+ +
Sbjct: 62  TSNLLSLKDIVELSLSVAMDAAKLNNENIKKLSMSGNIVGKRDRIALHDCVKTTDRTIYE 121

Query: 131 LDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEKRVLKLIETPHI 190
           LDK ++D  EYP KKSL  YADDLKTFLSS +TNQ TCL+G S D+ EKRVL+LIE  H 
Sbjct: 122 LDKAIQDFSEYPNKKSLASYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENTHN 181

Query: 191 HVTKLCSNVLALIKNLTDTDLTLRG----LVPEHSNKIAS-SWHADDSKIVLFSDVTNEE 250
            VTKLCSN LAL+K LT TD+ L      +V +   KI S S   DD KIVLFS   N+E
Sbjct: 182 QVTKLCSNALALVKKLT-TDVALTDEKSLVVHDFPYKITSISSQIDDPKIVLFS---NQE 241

Query: 251 EGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSEAVAAAP 310
           +GE+R RE LE+GIKWP+WMSI D+ LL+SSSE  AADVVVAADG+GN+ TVSEAV AAP
Sbjct: 242 DGESRRREDLENGIKWPEWMSIQDQMLLDSSSE-EAADVVVAADGTGNYKTVSEAVKAAP 301

Query: 311 NKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTFNSATVA 370
           +K+S RY+IKIKAGVY+ENV+VP SK+NIMFWGDGRSNTIIT  RS G+GW+TFNSATV 
Sbjct: 302 SKNS-RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITADRSHGSGWSTFNSATVV 361

Query: 371 AVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNC 430
           AVGDGFLARDIT +NTAG+AN QAVALRVGSDHSAFYRC + AYQDTLYVHSNRQFFV C
Sbjct: 362 AVGDGFLARDITFQNTAGSANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVKC 421

Query: 431 VIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKCTIKATS 490
           V+AGT+DFIFGNAA VIQ+S LTPR+P PNQ NMVTAQ R + NQNTGIVIQKC IKATS
Sbjct: 422 VVAGTVDFIFGNAAAVIQNSDLTPRKPGPNQNNMVTAQSRTDLNQNTGIVIQKCRIKATS 481

Query: 491 DLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKG 550
           DLEPVIKEFPSFLGRPW+EYARVVVMQT ITNVID +GW+ W+GD  +K  +FAEYDN G
Sbjct: 482 DLEPVIKEFPSFLGRPWEEYARVVVMQTSITNVIDKEGWSTWNGD--IKKPYFAEYDNNG 541

Query: 551 DGANTCCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
            GA+T  RV WS +IN  +AK FTA  FI GA WLPST FPYQLGL
Sbjct: 542 AGADTSGRVSWSLVINEAEAKTFTAEPFIDGAGWLPSTGFPYQLGL 579

BLAST of Tan0010927 vs. NCBI nr
Match: XP_004139620.2 (pectinesterase [Cucumis sativus] >KGN44621.1 hypothetical protein Csa_015743 [Cucumis sativus])

HSP 1 Score: 755.4 bits (1949), Expect = 3.8e-214
Identity = 401/586 (68.43%), Postives = 473/586 (80.72%), Query Frame = 0

Query: 11  KNELLVLALFA-TLLTNAIVVISEVNYDENSHNNVGVVKHSCATTLYPQLCHSTISSIMG 70
           +N  L LAL A  LLTNAIVV    N +ENS     V++ SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENNKLSLALLAILLLTNAIVV--RANSNENS-----VIETSCAITLYPQLCHSTISSIVG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRDCIETTDKTLDD 130
            S  LS+KDI E+SLSVA+DAAKHN++NIKKL++S ++++KR+++ L DC+ETTD+T+ +
Sbjct: 62  TSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYE 121

Query: 131 LDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEKRVLKLIETPHI 190
           L K +E  REYP K+SLTLYADDLKTFLSS +TNQ TCL+G S D+ EKRVL+LIE  HI
Sbjct: 122 LGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHI 181

Query: 191 HVTKLCSNVLALIKNLTDTDLTLRG----LVPEHSNKIAS-SWHADDSKIVLFSDVTNEE 250
           HVTKLCSN LAL++ LT TD+ +      +V +   KI S     DD KIVLFS   N+E
Sbjct: 182 HVTKLCSNALALVQKLT-TDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFS---NQE 241

Query: 251 EGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSEAVAAAP 310
           E ENR RE LEDGIKWPKWMSI D++LLESSSE AAA+ VVAADGSGN+ TV+EAVAAAP
Sbjct: 242 EDENRRREELEDGIKWPKWMSIEDQKLLESSSE-AAAEAVVAADGSGNYKTVAEAVAAAP 301

Query: 311 NKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTFNSATVA 370
           +K+SKRY+IKIKAG Y ENV+VP SK+NIMFWGDGRSNT I  +RS GTGW+TF SAT+A
Sbjct: 302 SKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLA 361

Query: 371 AVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNC 430
           AVGDGFLARDIT +N AGAAN QAVALRVGSDHSAFYRC + AYQDTLYVHSNRQFFVNC
Sbjct: 362 AVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNC 421

Query: 431 VIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKCTIKATS 490
           ++AGT+DFIFGNAA V Q+S +TPR+P P+Q+NMVTAQ R + NQNTGIVIQKC IKATS
Sbjct: 422 IVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATS 481

Query: 491 DLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKG 550
           DLEPVI+EFPSFLGRPW+EYARVVVMQT I+NVID +GW+ W+G    K+ ++AEYDN G
Sbjct: 482 DLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYAEYDNNG 541

Query: 551 DGANTCCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
            GA+   RV WS +I+  QAK FTAG FI GADWL ST FPYQL L
Sbjct: 542 AGADISGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 573

BLAST of Tan0010927 vs. ExPASy TrEMBL
Match: A0A1S3C861 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497582 PE=3 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 7.6e-221
Identity = 409/586 (69.80%), Postives = 476/586 (81.23%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEVNYDENSHNNVGVVKHSCATTLYPQLCHSTISSIMG 70
           +N  L LALFAT LLTNAI+V +  N +    N   +++ SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRDCIETTDKTLDD 130
            S  LS+KDIV++SLSVA+DAAK N+ NIK  LMS ++++KR+R+ L DC+++TD+T+ +
Sbjct: 62  TSNLLSLKDIVQLSLSVAMDAAKLNNGNIKN-LMSFNNVSKRDRIPLHDCVKSTDRTIYE 121

Query: 131 LDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEKRVLKLIETPHI 190
           LDK +ED REYP KKSLT YADDLKTFLSS +TNQ TCL+GF  D+ EK VL++IE  HI
Sbjct: 122 LDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTHI 181

Query: 191 HVTKLCSNVLALIKNLTDTDLTL----RGLVPEHSNKIAS-SWHADDSKIVLFSDVTNEE 250
           H TKLCSN LAL+K LT T++ L      +V +   KI S S   +D KIVLFS   N+E
Sbjct: 182 HATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFS---NQE 241

Query: 251 EGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSEAVAAAP 310
           EGEN  RE LE+GIKWP+WMSI D++LLESSS  AAADVVVA DGSGN+ TVSEAV AAP
Sbjct: 242 EGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAP 301

Query: 311 NKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTFNSATVA 370
           +K+SKRY+IKIKAGVY+ENV+VP SK+NIMFWGDGRSNTIIT  RS G+GW+TFNSATV 
Sbjct: 302 SKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVV 361

Query: 371 AVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNC 430
           AVGDGFLARDI+ ENTAG AN QAVALRVGSDHSAFYRCG+  YQDTLYVHSNRQFFVNC
Sbjct: 362 AVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNC 421

Query: 431 VIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKCTIKATS 490
           V+AGT+DF+FGNAA VIQ+S LTPR+P PNQ+NMVTAQ R + NQNTGIVIQKC IKATS
Sbjct: 422 VVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATS 481

Query: 491 DLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKG 550
           DLEPVIKEFPSFLGRPW+EYARVVVMQT ITNVID KGW+ W+GD  +K  +FAEYDN G
Sbjct: 482 DLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNG 541

Query: 551 DGANTCCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
            GANT  RV WS +IN   AK FTA +FI GADWLPST FPYQLGL
Sbjct: 542 AGANTSGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 580

BLAST of Tan0010927 vs. ExPASy TrEMBL
Match: A0A5D3E4E8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold343G001330 PE=3 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 1.3e-220
Identity = 404/581 (69.54%), Postives = 471/581 (81.07%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEVNYDENSHNNVGVVKHSCATTLYPQLCHSTISSIMG 70
           +N  L LALFAT LLTNAI+V +  N +    N   +++ SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRDCIETTDKTLDD 130
            S  LS+KDIV++SLSVA+DAAK N+ NIKKL MS+++++KR+R+ L DC+++TD+T+ +
Sbjct: 62  TSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIYE 121

Query: 131 LDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEKRVLKLIETPHI 190
           LDK +ED REYP KKSLT YADDLKTFLSS +TNQ TCL+GF  D+ EKRVL++IE  HI
Sbjct: 122 LDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTHI 181

Query: 191 HVTKLCSNVLALIKNLTDTDLTLRGLVPEHSNKIASSWHADDSKIVLFSDVTNEEEGENR 250
           H TKLCSN LAL+K LT    T   L+ E + K +    +     VLFS   N+EEGEN 
Sbjct: 182 HATKLCSNALALVKKLT----TEIALIDEKNLKKSRKARSH----VLFS---NQEEGENG 241

Query: 251 GREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSEAVAAAPNKSSK 310
            RE LE+GIKWP+WMSI D++LLESSS  AA DVVVA DGSGN+ TVSEAV AAP+K+SK
Sbjct: 242 RREDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKNSK 301

Query: 311 RYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTFNSATVAAVGDG 370
           RY+IKIKAGVY+ENV+VP SK+NIMFWGDGRSNTIIT  RS G+GW+TFNSATV AVGDG
Sbjct: 302 RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDG 361

Query: 371 FLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVIAGT 430
           FLARDI+ ENTAG AN QAVALRVGSDHSAFYRCG+  YQDTLYVHSNRQFFVNCV+AGT
Sbjct: 362 FLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGT 421

Query: 431 IDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKCTIKATSDLEPV 490
           +DF+FGNAA VIQ+S LTPR+P PNQ+NMVTAQ R + NQNTGIVIQKC IKATSDLEPV
Sbjct: 422 VDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPV 481

Query: 491 IKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDGANT 550
           IKEFPSFLGRPW+EYARVVVMQT ITNVID KGW+ W+GD  +K  +FAEYDN G GANT
Sbjct: 482 IKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNGAGANT 541

Query: 551 CCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
             RV WS +IN   AK FTA +FI GADWLPST FPYQLGL
Sbjct: 542 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 569

BLAST of Tan0010927 vs. ExPASy TrEMBL
Match: A0A5A7SNA2 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G001370 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 7.2e-219
Identity = 398/581 (68.50%), Postives = 465/581 (80.03%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEVNYDENSHNNVGVVKHSCATTLYPQLCHSTISSIMG 70
           +N  L LALFAT LLTNAI+V +  N +    N   +++ SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRDCIETTDKTLDD 130
            S  LS+KDIV++SLSVA+DAAK N+ NIKKL MS+++++KR+R+ L DC+++TD+T+ +
Sbjct: 62  TSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIYE 121

Query: 131 LDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEKRVLKLIETPHI 190
           LDK +ED REYP KKSLT YADDLKTFLSS +TNQ TCL+GF  D+ EKRVL++IE  HI
Sbjct: 122 LDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTHI 181

Query: 191 HVTKLCSNVLALIKNLTDTDLTLRGLVPEHSNKIASSWHADDSKIVLFSDVTNEEEGENR 250
           H TKLCSN LAL+K LT T++ L                 D+  +       + EEGEN 
Sbjct: 182 HATKLCSNALALVKKLT-TEIAL----------------IDEKNLKKSRKARSHEEGENG 241

Query: 251 GREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSEAVAAAPNKSSK 310
            RE LE+GIKWP+WMSI D++LLESSS  AA DVVVA DGSGN+ TVSEAV AAP+K+SK
Sbjct: 242 RREDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKNSK 301

Query: 311 RYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTFNSATVAAVGDG 370
           RY+IKIKAGVY+ENV+VP SK+NIMFWGDGRSNTIIT  RS G+GW+TFNSATV AVGDG
Sbjct: 302 RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDG 361

Query: 371 FLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVIAGT 430
           FLARDI+ ENTAG AN QAVALRVGSDHSAFYRCG+  YQDTLYVHSNRQFFVNCV+AGT
Sbjct: 362 FLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGT 421

Query: 431 IDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKCTIKATSDLEPV 490
           +DF+FGNAA VIQ+S LTPR+P PNQ+NMVTAQ R + NQNTGIVIQKC IKATSDLEPV
Sbjct: 422 VDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPV 481

Query: 491 IKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDGANT 550
           IKEFPSFLGRPW+EYARVVVMQT ITNVID KGW+ W+GD  +K  +FAEYDN G GANT
Sbjct: 482 IKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNGAGANT 541

Query: 551 CCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
             RV WS +IN   AK FTA +FI GADWLPST FPYQLGL
Sbjct: 542 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 563

BLAST of Tan0010927 vs. ExPASy TrEMBL
Match: A0A0A0K6S6 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343850 PE=3 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 7.9e-218
Identity = 409/586 (69.80%), Postives = 475/586 (81.06%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEVNYDENSHNNVGVVKHSCATTLYPQLCHSTISSIMG 70
           +N  L LALFAT LLTNAI+V +  N +   +N + V++ SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMNNILSVIETSCAITLYPQLCHSTISSILG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRDCIETTDKTLDD 130
            S  LS+KDIVE+SLSVA+DAAK N+ENIKKL MS + + KR+R+AL DC++TTD+T+ +
Sbjct: 62  TSNLLSLKDIVELSLSVAMDAAKLNNENIKKLSMSGNIVGKRDRIALHDCVKTTDRTIYE 121

Query: 131 LDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEKRVLKLIETPHI 190
           LDK ++D  EYP KKSL  YADDLKTFLSS +TNQ TCL+G S D+ EKRVL+LIE  H 
Sbjct: 122 LDKAIQDFSEYPNKKSLASYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENTHN 181

Query: 191 HVTKLCSNVLALIKNLTDTDLTLRG----LVPEHSNKIAS-SWHADDSKIVLFSDVTNEE 250
            VTKLCSN LAL+K LT TD+ L      +V +   KI S S   DD KIVLFS   N+E
Sbjct: 182 QVTKLCSNALALVKKLT-TDVALTDEKSLVVHDFPYKITSISSQIDDPKIVLFS---NQE 241

Query: 251 EGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSEAVAAAP 310
           +GE+R RE LE+GIKWP+WMSI D+ LL+SSSE  AADVVVAADG+GN+ TVSEAV AAP
Sbjct: 242 DGESRRREDLENGIKWPEWMSIQDQMLLDSSSE-EAADVVVAADGTGNYKTVSEAVKAAP 301

Query: 311 NKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTFNSATVA 370
           +K+S RY+IKIKAGVY+ENV+VP SK+NIMFWGDGRSNTIIT  RS G+GW+TFNSATV 
Sbjct: 302 SKNS-RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITADRSHGSGWSTFNSATVV 361

Query: 371 AVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNC 430
           AVGDGFLARDIT +NTAG+AN QAVALRVGSDHSAFYRC + AYQDTLYVHSNRQFFV C
Sbjct: 362 AVGDGFLARDITFQNTAGSANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVKC 421

Query: 431 VIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKCTIKATS 490
           V+AGT+DFIFGNAA VIQ+S LTPR+P PNQ NMVTAQ R + NQNTGIVIQKC IKATS
Sbjct: 422 VVAGTVDFIFGNAAAVIQNSDLTPRKPGPNQNNMVTAQSRTDLNQNTGIVIQKCRIKATS 481

Query: 491 DLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKG 550
           DLEPVIKEFPSFLGRPW+EYARVVVMQT ITNVID +GW+ W+GD  +K  +FAEYDN G
Sbjct: 482 DLEPVIKEFPSFLGRPWEEYARVVVMQTSITNVIDKEGWSTWNGD--IKKPYFAEYDNNG 541

Query: 551 DGANTCCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
            GA+T  RV WS +IN  +AK FTA  FI GA WLPST FPYQLGL
Sbjct: 542 AGADTSGRVSWSLVINEAEAKTFTAEPFIDGAGWLPSTGFPYQLGL 579

BLAST of Tan0010927 vs. ExPASy TrEMBL
Match: A0A0A0K9Y3 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343870 PE=3 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.8e-214
Identity = 401/586 (68.43%), Postives = 473/586 (80.72%), Query Frame = 0

Query: 11  KNELLVLALFA-TLLTNAIVVISEVNYDENSHNNVGVVKHSCATTLYPQLCHSTISSIMG 70
           +N  L LAL A  LLTNAIVV    N +ENS     V++ SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENNKLSLALLAILLLTNAIVV--RANSNENS-----VIETSCAITLYPQLCHSTISSIVG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRDCIETTDKTLDD 130
            S  LS+KDI E+SLSVA+DAAKHN++NIKKL++S ++++KR+++ L DC+ETTD+T+ +
Sbjct: 62  TSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYE 121

Query: 131 LDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEKRVLKLIETPHI 190
           L K +E  REYP K+SLTLYADDLKTFLSS +TNQ TCL+G S D+ EKRVL+LIE  HI
Sbjct: 122 LGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHI 181

Query: 191 HVTKLCSNVLALIKNLTDTDLTLRG----LVPEHSNKIAS-SWHADDSKIVLFSDVTNEE 250
           HVTKLCSN LAL++ LT TD+ +      +V +   KI S     DD KIVLFS   N+E
Sbjct: 182 HVTKLCSNALALVQKLT-TDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFS---NQE 241

Query: 251 EGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSEAVAAAP 310
           E ENR RE LEDGIKWPKWMSI D++LLESSSE AAA+ VVAADGSGN+ TV+EAVAAAP
Sbjct: 242 EDENRRREELEDGIKWPKWMSIEDQKLLESSSE-AAAEAVVAADGSGNYKTVAEAVAAAP 301

Query: 311 NKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTFNSATVA 370
           +K+SKRY+IKIKAG Y ENV+VP SK+NIMFWGDGRSNT I  +RS GTGW+TF SAT+A
Sbjct: 302 SKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLA 361

Query: 371 AVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNC 430
           AVGDGFLARDIT +N AGAAN QAVALRVGSDHSAFYRC + AYQDTLYVHSNRQFFVNC
Sbjct: 362 AVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNC 421

Query: 431 VIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKCTIKATS 490
           ++AGT+DFIFGNAA V Q+S +TPR+P P+Q+NMVTAQ R + NQNTGIVIQKC IKATS
Sbjct: 422 IVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATS 481

Query: 491 DLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKG 550
           DLEPVI+EFPSFLGRPW+EYARVVVMQT I+NVID +GW+ W+G    K+ ++AEYDN G
Sbjct: 482 DLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYAEYDNNG 541

Query: 551 DGANTCCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
            GA+   RV WS +I+  QAK FTAG FI GADWL ST FPYQL L
Sbjct: 542 AGADISGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 573

BLAST of Tan0010927 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 583.2 bits (1502), Expect = 2.4e-166
Identity = 319/594 (53.70%), Postives = 408/594 (68.69%), Query Frame = 0

Query: 10  KKNELLVL--ALFATLLTNAIVVISEVNYDEN-----SHNNVGVVKHSCATTLYPQLCHS 69
           KKN+ LVL  A  A L   A+  IS      N     S ++  V++ SC++T YP+LC S
Sbjct: 15  KKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCIS 74

Query: 70  TISSIMGASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRDCIET 129
            + +  G  E  S KD++E S+++ I A +HN+  +KKL+     LT RE+ AL DC+ET
Sbjct: 75  AVVT-AGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLET 134

Query: 130 TDKTLDDLDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEKRVLK 189
            D+TLD+L + VEDL  YP KK+L  +A DLKT +SS +TNQETCL+GFS D+A+K+V K
Sbjct: 135 IDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRK 194

Query: 190 LIETPHIHVTKLCSNVLALIKNLTDTDLT---LRGLVPEHSNKIASSWHADDSKIVLFSD 249
            +    IHV  +CSN LA+IKN+TDTD+     +  +  ++ K+      ++ +  +  D
Sbjct: 195 ALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKE----ENQETTVAVD 254

Query: 250 VTNEEEGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSEA 309
           +    E ++ G         WP W+S  D RLL+ S     AD  VAADGSG F TV+ A
Sbjct: 255 IAGAGELDSEG---------WPTWLSAGDRRLLQGS--GVKADATVAADGSGTFKTVAAA 314

Query: 310 VAAAPNKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTFN 369
           VAAAP  S+KRYVI IKAGVY+ENVEV + KKNIMF GDGR+ TIITGSR+   G TTF+
Sbjct: 315 VAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFH 374

Query: 370 SATVAAVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQ 429
           SATVAAVG+ FLARDIT +NTAG + HQAVALRVGSD SAFY C + AYQDTLYVHSNRQ
Sbjct: 375 SATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQ 434

Query: 430 FFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKCT 489
           FFV C+IAGT+DFIFGNAAVV+QD  +  RRP+  Q+NMVTAQGR + NQNTGIVIQKC 
Sbjct: 435 FFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCR 494

Query: 490 IKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAE 549
           I ATSDL+ V   FP++LGRPWKEY++ V+MQ+ I++VI P+GW+ W+G FAL TL + E
Sbjct: 495 IGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYRE 554

Query: 550 YDNKGDGANTCCRVEWSS---LINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
           Y N G GA T  RV+W     +  A +A+ +TAGQFI G  WL ST FP+ LGL
Sbjct: 555 YSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Tan0010927 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 576.2 bits (1484), Expect = 2.9e-164
Identity = 321/600 (53.50%), Postives = 407/600 (67.83%), Query Frame = 0

Query: 4   TKVSLKKKNELLVL--ALFATLLTNAIVVISEVNYDENSHNNV--------GVVKHSCAT 63
           +K S  K N+ L+L  A  A LL  +IV I+    ++N +  +         ++K  C++
Sbjct: 9   SKFSDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSS 68

Query: 64  TLYPQLCHSTISSIMGASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLSDLTKRER 123
           TLYP+LC S +++  G  E  S K+++E SL++   A KHN+  +KKL+     LT RE 
Sbjct: 69  TLYPELCFSAVAA-TGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREV 128

Query: 124 MALRDCIETTDKTLDDLDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSG 183
            AL DC+ET D+TLD+L   VEDL +YPK+KSL  +ADDLKT +SS +TNQ TCL+GFS 
Sbjct: 129 TALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSY 188

Query: 184 DEAEKRVLKLIETPHIHVTKLCSNVLALIKNLTDTDLTLRGLVPEHSNKIASSWHADDSK 243
           D+A+++V K +    +HV  +CSN LA+IKN+T+TD             IA+    D S 
Sbjct: 189 DDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETD-------------IANFELRDKS- 248

Query: 244 IVLFSDVTNEEEGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAADVVVAADGSGNF 303
               S  TN    + +   G  D   WPKW+S+ D RLL+ S+    AD  VA DGSG+F
Sbjct: 249 ----STFTNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGST--IKADATVADDGSGDF 308

Query: 304 GTVSEAVAAAPNKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGT 363
            TV+ AVAAAP KS+KR+VI IKAGVY+ENVEV + K NIMF GDGR  TIITGSR+   
Sbjct: 309 TTVAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVD 368

Query: 364 GWTTFNSATVAAVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLY 423
           G TTF+SATVAAVG+ FLARDIT +NTAG + HQAVALRVGSD SAFY+C + AYQDTLY
Sbjct: 369 GSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLY 428

Query: 424 VHSNRQFFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGI 483
           VHSNRQFFV C I GT+DFIFGNAA V+QD  +  RRP+  Q+NMVTAQGR + NQNTGI
Sbjct: 429 VHSNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGI 488

Query: 484 VIQKCTIKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALK 543
           VIQ C I  TSDL  V   FP++LGRPWKEY+R V+MQ+DI++VI P+GW  WSG FAL 
Sbjct: 489 VIQNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALD 548

Query: 544 TLFFAEYDNKGDGANTCCRVEWS--SLINAD-QAKAFTAGQFISGADWLPSTAFPYQLGL 591
           TL + EY N+G GA T  RV+W    +I +D +A+ FTAGQFI G  WL ST FP+ L L
Sbjct: 549 TLTYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Tan0010927 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 371.7 bits (953), Expect = 1.1e-102
Identity = 218/553 (39.42%), Postives = 311/553 (56.24%), Query Frame = 0

Query: 47  VKHSCATTLYPQLCHSTISSIMGASEFLSVKDIVEISLSVAIDAAKHNHENIKKLLMSLS 106
           +  +C+ +LYP LC  T+    G S      +++ IS +  +   K +        ++ +
Sbjct: 75  ISRTCSKSLYPNLCIDTLLDFPG-SLTADENELIHISFNATLQ--KFSKALYTSSTITYT 134

Query: 107 DLTKRERMALRDCIETTDKTLDDLDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTNQET 166
            +  R R A   C+E  D ++D L + +  +      +S      D+ T+LSS MTN +T
Sbjct: 135 QMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDES----HSDVMTWLSSAMTNHDT 194

Query: 167 CLEGF-----SGDEAEKRVLKLIETPHIHVTKLCSNVLALIKNLTDTDLTLRGLVPEHSN 226
           C +GF      G E + +V+  ++     ++++ SN LA+                  + 
Sbjct: 195 CTDGFDEIEGQGGEVKDQVIGAVK----DLSEMVSNCLAIF-----------------AG 254

Query: 227 KIASSWHADDSKIVLFSDVTNEEEGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAA 286
           K+               D++      NR   G E+  + P W+   D  LL + + A  A
Sbjct: 255 KV--------------KDLSGVPVVNNRKLLGTEETEELPNWLKREDRELLGTPTSAIQA 314

Query: 287 DVVVAADGSGNFGTVSEAVAAAPNKSSKRYVIKIKAGVY-KENVEVPRSKKNIMFWGDGR 346
           D+ V+ DGSG F T++EA+  AP  SS+R+VI +KAG Y +EN++V R K N+MF GDG+
Sbjct: 315 DITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGK 374

Query: 347 SNTIITGSRSFGTGWTTFNSATVAAVGDGFLARDITIENTAGAANHQAVALRVGSDHSAF 406
             T+ITG +S     TTF++AT AA G GF+ RD+T EN AG A HQAVALRVG DH+  
Sbjct: 375 GKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVV 434

Query: 407 YRCGIQAYQDTLYVHSNRQFFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVT 466
           YRC I  YQD LYVHSNRQFF  C I GT+DFIFGNAAV++Q   +  R+P   Q+  +T
Sbjct: 435 YRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITIT 494

Query: 467 AQGRIEANQNTGIVIQKCTIKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDP 526
           AQ R + NQNTGI I  C + AT DLE     +P++LGRPWK Y+RVV M +D+ + IDP
Sbjct: 495 AQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDP 554

Query: 527 KGWAPWSGDFALKTLFFAEYDNKGDGANTCCRVEWSS---LINADQAKAFTAGQFISGAD 586
           +GW  W+G FAL +L++ EY NKG G+    RV+W     + +  +A  FT  QFISG+ 
Sbjct: 555 RGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSS 585

Query: 587 WLPSTAFPYQLGL 591
           WLPST   +  GL
Sbjct: 615 WLPSTGVSFFSGL 585

BLAST of Tan0010927 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 369.0 bits (946), Expect = 7.1e-102
Identity = 235/574 (40.94%), Postives = 324/574 (56.45%), Query Frame = 0

Query: 20  FATLLTNAIVVISEVNYDENSHNNVGVVKHSCATTLYPQLCHSTISSIMGASEFLSVKDI 79
           F   + N +++   V    + +N    VK  C+ T  P+ C   ++         S  + 
Sbjct: 4   FRAYIINFVILCILVASTVSGYNQKD-VKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEF 63

Query: 80  VEISLSVAIDAAKHNHENIKKLLMSLSDLTKRERMALRDCIETTDKTLDDLDKVVEDLRE 139
           ++IS+ + +D A     +   L     D   RE+ A  DCI+  D T+  +++ ++    
Sbjct: 64  LKISMKLVLDRAILAKTHAFTLGPKCRD--TREKAAWEDCIKLYDLTVSKINETMD---- 123

Query: 140 YPKKKSLTLYADDLKTFLSSTMTNQETCLEGFSGDEAEKRVLKLIETPHIHVTKLCSNVL 199
            P  K   L   D +T+LS+ +TN +TC  GF        VL L+     +V+ L  N L
Sbjct: 124 -PNVKCSKL---DAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSN---NVSNLLCNTL 183

Query: 200 ALIKNLTDTDLTLRGLVPEHSNKIASSWHADDSKIVLFSDVTNEEEGENRGREGLEDGIK 259
           A+ K                               V F+    E++G             
Sbjct: 184 AINK-------------------------------VPFNYTPPEKDG------------- 243

Query: 260 WPKWMSISDERLLESSSEAAAADVVVAADGSGNFGTVSEAVAAAPNKSSKRYVIKIKAGV 319
           +P W+   D +LL+SS+    A  VVA DGSGNF T+ EA+ AA    S R+VI +K GV
Sbjct: 244 FPSWVKPGDRKLLQSSTPKDNA--VVAKDGSGNFKTIKEAIDAA--SGSGRFVIYVKQGV 303

Query: 320 YKENVEVPRSKKNIMFWGDGRSNTIITGSRSFGTGWTTFNSATVAAVGDGFLARDITIEN 379
           Y EN+E+   KKN+M  GDG   TIITGS+S G G TTFNSATVAAVGDGF+AR IT  N
Sbjct: 304 YSENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRN 363

Query: 380 TAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVIAGTIDFIFGNAAV 439
           TAGA+N QAVALR GSD S FY+C  +AYQDTLYVHSNRQF+ +C + GT+DFIFGNAA 
Sbjct: 364 TAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAA 423

Query: 440 VIQDSVLTPRRPDPNQQNMVTAQGRIEANQNTGIVIQKCTIKATSDLEPVIKEFPSFLGR 499
           V+Q+  +  RRP  ++ N +TAQGR + NQNTGI+I    + A SDL PV+    ++LGR
Sbjct: 424 VLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGR 483

Query: 500 PWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDGANTCCRVEWSS-- 559
           PW++Y+R V M+T + ++IDP+GW  W G+FALKTLF+AE+ N G GA+T  RV W    
Sbjct: 484 PWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFR 511

Query: 560 -LINADQAKAFTAGQFISGADWLPSTAFPYQLGL 591
            L +A +A  FT G F++G  W+PS+  P+  GL
Sbjct: 544 VLGSASEASKFTVGTFLAGGSWIPSSV-PFTSGL 511

BLAST of Tan0010927 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 368.2 bits (944), Expect = 1.2e-101
Identity = 213/551 (38.66%), Postives = 309/551 (56.08%), Query Frame = 0

Query: 48  KHSCATTLYPQLCHSTISSIMGASEFLSVKDIVEI---SLSVAIDAAKHNHENIKKLLMS 107
           KH   +  +  L  ST+ S      F S  DI E+   +L+  I     +  N   LL  
Sbjct: 6   KHIILSLTFLLLFLSTVFSSHVTQRFTSSDDITELVVTALNQTISNVNLSSSNFSDLLQR 65

Query: 108 L-SDLTKRERMALRDCIETTDKTLDDLDKVVEDLREYPKKKSLTLYADDLKTFLSSTMTN 167
           L S+L+ R+  A  DC+E  D T+ DL   +  LR +  +        ++K  LS+ MTN
Sbjct: 66  LGSNLSHRDLCAFDDCLELLDDTVFDLTTAISKLRSHSPE------LHNVKMLLSAAMTN 125

Query: 168 QETCLEGFSGDEAEKRVLKLIETPHIHVTKLCSNVLALIKNLTDTDLTLRGLVPEHSNKI 227
             TCL+GF+  + +                         +NL + D    G+       +
Sbjct: 126 TRTCLDGFASSDND-------------------------ENLNNNDNKTYGVAESLKESL 185

Query: 228 AS-SWHADDSKIVLFSDVTNEEEGENRGREGLEDGIKWPKWMSISDERLLESSSEAAAAD 287
            + S H  DS + +  ++     G+      +++ + +P W+S SD  LL+   +    +
Sbjct: 186 FNISSHVSDS-LAMLENIPGHIPGK------VKEDVGFPMWVSGSDRNLLQDPVDETKVN 245

Query: 288 VVVAADGSGNFGTVSEAVAAAPNKSSKRYVIKIKAGVYKENVEVPRSKKNIMFWGDGRSN 347
           +VVA +G+GN+ T+ EA++AAPN S  R+VI IK G Y EN+E+PR K  IMF GDG   
Sbjct: 246 LVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIGR 305

Query: 348 TIITGSRSFGTGWTTFNSATVAAVGDGFLARDITIENTAGAANHQAVALRVGSDHSAFYR 407
           T+I  +RS+  GWT F+SATV   G GF+A+D++  N AG   HQAVALR  SD SA+YR
Sbjct: 306 TVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSSDLSAYYR 365

Query: 408 CGIQAYQDTLYVHSNRQFFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQ 467
           C  ++YQDT+YVHS++QF+  C I GT+DFIFG+A+VV Q+  L  RRP+PNQ+ + TAQ
Sbjct: 366 CSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYTAQ 425

Query: 468 GRIEANQNTGIVIQKCTIKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKG 527
           GR  + + TGI I    I A  DL PV   F ++LGRPW+ Y+R V+M++ I +++DP G
Sbjct: 426 GRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVDPAG 485

Query: 528 WAPWSGDFALKTLFFAEYDNKGDGANTCCRVEW---SSLINADQAKAFTAGQFISGADWL 587
           W  W  DFAL+TL++ EY N+G G+N   RV+W     +   ++A  F+ G FI G  WL
Sbjct: 486 WLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNKWL 518

Query: 588 PSTAFPYQLGL 591
            ST  P+ L L
Sbjct: 546 NSTRIPFTLDL 518

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P839483.5e-17054.96Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
O048866.5e-16954.29Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
Q431431.2e-16552.51Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM... [more]
O490063.4e-16553.70Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
Q425344.1e-16353.50Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_008458040.21.6e-22069.80PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo][more]
TYK30782.12.7e-22069.54pectinesterase-like [Cucumis melo var. makuwa][more]
KAA0026177.11.5e-21868.50pectinesterase-like [Cucumis melo var. makuwa][more]
XP_011659194.11.6e-21769.80pectinesterase [Cucumis sativus] >KGN44619.1 hypothetical protein Csa_015815 [Cu... [more]
XP_004139620.23.8e-21468.43pectinesterase [Cucumis sativus] >KGN44621.1 hypothetical protein Csa_015743 [Cu... [more]
Match NameE-valueIdentityDescription
A0A1S3C8617.6e-22169.80Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497582 PE=3 SV=1[more]
A0A5D3E4E81.3e-22069.54Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold343G00133... [more]
A0A5A7SNA27.2e-21968.50Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G001370... [more]
A0A0A0K6S67.9e-21869.80Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343850 PE=3 SV=1[more]
A0A0A0K9Y31.8e-21468.43Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343870 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14310.12.4e-16653.70pectin methylesterase 3 [more]
AT1G53830.12.9e-16453.50pectin methylesterase 2 [more]
AT5G53370.11.1e-10239.42pectin methylesterase PCR fragment F [more]
AT2G45220.17.1e-10240.94Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02320.11.2e-10138.66Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 42..201
e-value: 6.1E-30
score: 115.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 47..201
e-value: 5.6E-27
score: 95.0
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 16..206
e-value: 1.1E-21
score: 75.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 45..207
e-value: 7.9E-32
score: 112.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 46..206
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 276..590
e-value: 1.2E-119
score: 401.4
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 282..575
e-value: 1.5E-135
score: 450.7
NoneNo IPR availablePANTHERPTHR31707:SF180PECTINESTERASEcoord: 14..589
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 14..589
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 50..206
e-value: 4.19666E-36
score: 130.636
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 426..435
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 277..588

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0010927.1Tan0010927.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity