Tan0010705 (gene) Snake gourd v1

Overview
NameTan0010705
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPectate lyase
LocationLG07: 56607071 .. 56608390 (+)
RNA-Seq ExpressionTan0010705
SyntenyTan0010705
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGGTTGTAGAATGATCAAGGTTGTGATGGCGCTGGCTTGCATGGCTGTGATGCTGCCAACTCTCAGAGCTGGAATTGCTGAATTTGATGAGTATTTGCTGCAAAAAGCTGATGAAGCGAAACAAGCTTCTCTTGAGGCGTTCCACCCTGACCCCATGAATGTGACTGAGCATTTGAACAATCAAGTTCATTTAGCCTTAGAGGGATCAGGGAATAGTAGTAGGAGGAGCCTGAGGAAATACAACGGCCCATGTTTGGCTACAAACCCAATCGACCGCTGCTGGCGATGTGACCCAAACTGGGCCAAGAATCGTAAGAAGCTTGCCTCTTGTGTGTTGGGTTTTGGCCGAAAAACCACCGGTGGGAAAAGAGGAAAAATCTACACAGTGATAGACCCGTCTGACAACGACGTCGTAAACCCTAAACCCGGAACCCTAAGACACGCAGCAATCCAAAAGCGACCGCTGTGGATCATATTTGGGCGGAGTATGATCATCAAGCTGTCGAAAGAGCTGTTAGTGGCGAGCCACAAGACCATCGATGCACGTGGAAAAAATATCCACATAGCATATGGAGCTGGACTTAGCGTTCAATTCTCGACGAATGTGATAATCCACGGGCTTCACATCCACCACGTTGTAGCAACCAGCGGTGGCATAATTCGAGACGCATACAACCATGTTGGGATTCGAACGGTGAGCGATGGAGATGGTATCTCTATCTTTGGATCCTCCAATATCTGGCTTGATCATCTCTCAATGTCCAACTGCCAGGATGGACTAATCGACGCAATCCAAGGTTCGACTGCAATCACAGTCTCTAACTGTCATTTCACTCACCATAACGACGTGATGTTGTTCGGTGCAAGTGATTCGTACCAAGGCGATTCCATCATGCAAGTCACTGTGGCATTCAACCATTTTGGGAAAGGGTTGATTCAGAGAATGCCCAGATGCCGATGGGGTTTCTTCCATGTCGTCAACAATGATTACACTCATTGGCTAATGTACGCCATTGGTGGAAGCCAACATCCAACGATTATCAGTCAGGGGAATCGTTTCATTGCGCCACCAAATCCTGCTGCCAGACAGATTACAAACAGAAACTATGCAACTGAGAGTGTGTGGAGAACATGGACATGGAGATCAGAAGGGGATCTCATGATGAATGGTGCCTATTTTGTTCAGTCTGGAAATCCAAGCAAAAGAAGGTCATACAGCAGATTTGACATGATCAAGGCTAAGCCTGGCACTTTTGTTAGAAGGCTTACACGTTTCGCTGGCGCCCTCAACTGTTATCTTGGTCGACCATGCTAA

mRNA sequence

ATGGATGGTTGTAGAATGATCAAGGTTGTGATGGCGCTGGCTTGCATGGCTGTGATGCTGCCAACTCTCAGAGCTGGAATTGCTGAATTTGATGAGTATTTGCTGCAAAAAGCTGATGAAGCGAAACAAGCTTCTCTTGAGGCGTTCCACCCTGACCCCATGAATGTGACTGAGCATTTGAACAATCAAGTTCATTTAGCCTTAGAGGGATCAGGGAATAGTAGTAGGAGGAGCCTGAGGAAATACAACGGCCCATGTTTGGCTACAAACCCAATCGACCGCTGCTGGCGATGTGACCCAAACTGGGCCAAGAATCGTAAGAAGCTTGCCTCTTGTGTGTTGGGTTTTGGCCGAAAAACCACCGGTGGGAAAAGAGGAAAAATCTACACAGTGATAGACCCGTCTGACAACGACGTCGTAAACCCTAAACCCGGAACCCTAAGACACGCAGCAATCCAAAAGCGACCGCTGTGGATCATATTTGGGCGGAGTATGATCATCAAGCTGTCGAAAGAGCTGTTAGTGGCGAGCCACAAGACCATCGATGCACGTGGAAAAAATATCCACATAGCATATGGAGCTGGACTTAGCGTTCAATTCTCGACGAATGTGATAATCCACGGGCTTCACATCCACCACGTTGTAGCAACCAGCGGTGGCATAATTCGAGACGCATACAACCATGTTGGGATTCGAACGGTGAGCGATGGAGATGGTATCTCTATCTTTGGATCCTCCAATATCTGGCTTGATCATCTCTCAATGTCCAACTGCCAGGATGGACTAATCGACGCAATCCAAGGTTCGACTGCAATCACAGTCTCTAACTGTCATTTCACTCACCATAACGACGTGATGTTGTTCGGTGCAAGTGATTCGTACCAAGGCGATTCCATCATGCAAGTCACTGTGGCATTCAACCATTTTGGGAAAGGGTTGATTCAGAGAATGCCCAGATGCCGATGGGGTTTCTTCCATGTCGTCAACAATGATTACACTCATTGGCTAATGTACGCCATTGGTGGAAGCCAACATCCAACGATTATCAGTCAGGGGAATCGTTTCATTGCGCCACCAAATCCTGCTGCCAGACAGATTACAAACAGAAACTATGCAACTGAGAGTGTGTGGAGAACATGGACATGGAGATCAGAAGGGGATCTCATGATGAATGGTGCCTATTTTGTTCAGTCTGGAAATCCAAGCAAAAGAAGGTCATACAGCAGATTTGACATGATCAAGGCTAAGCCTGGCACTTTTGTTAGAAGGCTTACACGTTTCGCTGGCGCCCTCAACTGTTATCTTGGTCGACCATGCTAA

Coding sequence (CDS)

ATGGATGGTTGTAGAATGATCAAGGTTGTGATGGCGCTGGCTTGCATGGCTGTGATGCTGCCAACTCTCAGAGCTGGAATTGCTGAATTTGATGAGTATTTGCTGCAAAAAGCTGATGAAGCGAAACAAGCTTCTCTTGAGGCGTTCCACCCTGACCCCATGAATGTGACTGAGCATTTGAACAATCAAGTTCATTTAGCCTTAGAGGGATCAGGGAATAGTAGTAGGAGGAGCCTGAGGAAATACAACGGCCCATGTTTGGCTACAAACCCAATCGACCGCTGCTGGCGATGTGACCCAAACTGGGCCAAGAATCGTAAGAAGCTTGCCTCTTGTGTGTTGGGTTTTGGCCGAAAAACCACCGGTGGGAAAAGAGGAAAAATCTACACAGTGATAGACCCGTCTGACAACGACGTCGTAAACCCTAAACCCGGAACCCTAAGACACGCAGCAATCCAAAAGCGACCGCTGTGGATCATATTTGGGCGGAGTATGATCATCAAGCTGTCGAAAGAGCTGTTAGTGGCGAGCCACAAGACCATCGATGCACGTGGAAAAAATATCCACATAGCATATGGAGCTGGACTTAGCGTTCAATTCTCGACGAATGTGATAATCCACGGGCTTCACATCCACCACGTTGTAGCAACCAGCGGTGGCATAATTCGAGACGCATACAACCATGTTGGGATTCGAACGGTGAGCGATGGAGATGGTATCTCTATCTTTGGATCCTCCAATATCTGGCTTGATCATCTCTCAATGTCCAACTGCCAGGATGGACTAATCGACGCAATCCAAGGTTCGACTGCAATCACAGTCTCTAACTGTCATTTCACTCACCATAACGACGTGATGTTGTTCGGTGCAAGTGATTCGTACCAAGGCGATTCCATCATGCAAGTCACTGTGGCATTCAACCATTTTGGGAAAGGGTTGATTCAGAGAATGCCCAGATGCCGATGGGGTTTCTTCCATGTCGTCAACAATGATTACACTCATTGGCTAATGTACGCCATTGGTGGAAGCCAACATCCAACGATTATCAGTCAGGGGAATCGTTTCATTGCGCCACCAAATCCTGCTGCCAGACAGATTACAAACAGAAACTATGCAACTGAGAGTGTGTGGAGAACATGGACATGGAGATCAGAAGGGGATCTCATGATGAATGGTGCCTATTTTGTTCAGTCTGGAAATCCAAGCAAAAGAAGGTCATACAGCAGATTTGACATGATCAAGGCTAAGCCTGGCACTTTTGTTAGAAGGCTTACACGTTTCGCTGGCGCCCTCAACTGTTATCTTGGTCGACCATGCTAA

Protein sequence

MDGCRMIKVVMALACMAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHLNNQVHLALEGSGNSSRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKTTGGKRGKIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASHKTIDARGKNIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDSIMQVTVAFNHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPGTFVRRLTRFAGALNCYLGRPC
Homology
BLAST of Tan0010705 vs. ExPASy Swiss-Prot
Match: Q9LFP5 (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 4.4e-162
Identity = 274/473 (57.93%), Postives = 346/473 (73.15%), Query Frame = 0

Query: 1   MDGCRMIKVVMALACMAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHL 60
           M+  R+ K++    C+AV++PT+R  I+E DEY  Q+ADEA++ +L+A+H DP  + +H 
Sbjct: 1   MEMVRLSKLMFTF-CIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHF 60

Query: 61  NNQVH-------------------------LALEGSGNSSRRSLR--------KYNGPCL 120
           + + +                           L  S NS+RRSLR        K  GPC 
Sbjct: 61  HERHYDNSTDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCT 120

Query: 121 ATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKTTGGKRGKIYTVIDPSDNDVVNPKPGTL 180
           A+NPID+CWRC  +WAK RKKL  CV GFG +TTGGKRG+IY V    D D+VNPKPGTL
Sbjct: 121 ASNPIDKCWRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTL 180

Query: 181 RHAAIQKRPLWIIFGRSMIIKLSKELLVASHKTIDARGKNIHIAYGAGLSVQFSTNVIIH 240
           RHA IQK PLWIIF   M I+L++ELL+ SHKTIDARG N+H+A+GAG+++QF  NVIIH
Sbjct: 181 RHAVIQKEPLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVIIH 240

Query: 241 GLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQ 300
           GLHIHH+  +SGG+IRD+ +H G+RT +DGDG+SI+GSSNIWLDH+SMS CQDGLIDAI 
Sbjct: 241 GLHIHHISESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIV 300

Query: 301 GSTAITVSNCHFTHHNDVMLFGASDSYQGDSIMQVTVAFNHFGKGLIQRMPRCRWGFFHV 360
           GST IT+SN HFTHHNDVML GA ++ + D  MQVTVA+NHFGKGL+QRMPR RWGF HV
Sbjct: 301 GSTGITISNSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHV 360

Query: 361 VNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN-PAARQITNRNYATESVWRTWTWRSEG 420
           VNNDYTHW +YAIGGSQ PTI+S GNRFIAPP+ P  R++T R+YA+E  W+ W WRS+ 
Sbjct: 361 VNNDYTHWELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDK 420

Query: 421 DLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPGTFVRRLTRFAGALNCYLGRPC 440
           D+ MNGAYF QSGNP  + +++R  MIK K G  V +LT++AGAL+C +GR C
Sbjct: 421 DVFMNGAYFRQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

BLAST of Tan0010705 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 6.4e-161
Identity = 273/476 (57.35%), Postives = 345/476 (72.48%), Query Frame = 0

Query: 1   MDGCRMIKVVMALACMAVMLPTLRAGIA-EFDEYLLQKADEAKQASLEAFHPDPMNVTEH 60
           M+  R+ K+V  + C+A ++PT+RA +A E DEY + KA+EA++ +L A+HPDP  + +H
Sbjct: 1   METARLFKLVCVI-CIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDH 60

Query: 61  LNNQ-----------------------VHLALEGSGNSSRRSL------------RKYNG 120
            + +                       V   +    NS+RRSL             K  G
Sbjct: 61  FHERHYDNSTDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTG 120

Query: 121 PCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKTTGGKRGKIYTVIDPSDNDVVNPKP 180
           PC A+NPID+CWRC P+WA+ RKKL  CV GFG +TTGGKRG+IY V  P D+D+VNP+P
Sbjct: 121 PCTASNPIDKCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRP 180

Query: 181 GTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASHKTIDARGKNIHIAYGAGLSVQFSTNV 240
           GTLRHA IQK PLWI+F   M I+LS+EL++ S KTIDARG N+HIAYGAG+++Q+  N+
Sbjct: 181 GTLRHAVIQKEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNI 240

Query: 241 IIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGISIFGSSNIWLDHLSMSNCQDGLID 300
           IIHGLH+HH+V +SGG+IRD+ NH G R  +DGDGISIFG++NIWLDH+SMS CQDGLID
Sbjct: 241 IIHGLHVHHIVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLID 300

Query: 301 AIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDSIMQVTVAFNHFGKGLIQRMPRCRWGF 360
           AI GSTAIT+SN HFTHHNDVML GA ++   D  MQVTVA+NHFGKGL+QRMPR RWGF
Sbjct: 301 AIMGSTAITISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGF 360

Query: 361 FHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPA-ARQITNRNYATESVWRTWTWR 420
            HVVNNDYTHW +YAIGGSQ PTI+S GNRFIAPP+    R++T R+YA+ES W+ W WR
Sbjct: 361 VHVVNNDYTHWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWR 420

Query: 421 SEGDLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPGTFVRRLTRFAGALNCYLGRPC 440
           SE D+ MN AYF QSGNP  + S+SR  MIK K G  V +LT++AGAL+C +G+ C
Sbjct: 421 SEKDVFMNNAYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of Tan0010705 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 3.0e-158
Identity = 265/416 (63.70%), Postives = 322/416 (77.40%), Query Frame = 0

Query: 25  AGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHLNNQVHLALEGSGNSSRRSLRKYN- 84
           A IAEFDEY  +K+  A+  + +A+ P P  VT H N  VH + E  GNS+RR+LR    
Sbjct: 22  ANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFE--GNSTRRNLRTNKL 81

Query: 85  GPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKTTGGKRGKIYTVIDPSDNDVVNPK 144
           G CLATNPIDRCWRC  NW+ NRK L  CV GFGRKTTGG  G+IY V DPSD+ + +PK
Sbjct: 82  GQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIYVVTDPSDDSLTDPK 141

Query: 145 PGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASHKTIDARGKNIHIAYGAGLSVQFSTN 204
            GTLR   IQ RPLWIIFG+SM+I+L +EL++ + KTID RG N+ IA GA L+VQF  N
Sbjct: 142 FGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQIAGGAQLTVQFVHN 201

Query: 205 VIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGISIFGSSNIWLDHLSMSNCQDGLI 264
           VIIHG+HIH +    GG+IRD+  H GIRT SDGDGISI GSSNIW+DH+S++ C DGLI
Sbjct: 202 VIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWIDHVSLARCSDGLI 261

Query: 265 DAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDSIMQVTVAFNHFGKGLIQRMPRCRWG 324
           D I GSTAIT+SNCH T H+DVML GASD+Y  D IMQVTVAFNHFG+GL+QRMPRCR+G
Sbjct: 262 DVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGRGLVQRMPRCRYG 321

Query: 325 FFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAARQITNRNYATESVWRTWTWR 384
           F HVVNNDYTHW+MYA+GGSQHPTIISQGNR+IAP   AA+++T R+YA  + W  WTW+
Sbjct: 322 FVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKRDYAEPAEWSKWTWK 381

Query: 385 SEGDLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPGTFVRRLTRFAGALNCYLGRPC 440
           S+GDL ++GA+FV+SG P + + YS+ D+IKAKPGTFV+RLTRF+GALNC     C
Sbjct: 382 SQGDLFVSGAFFVESGGPFENK-YSKKDLIKAKPGTFVQRLTRFSGALNCKENMEC 434

BLAST of Tan0010705 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 3.7e-153
Identity = 262/445 (58.88%), Postives = 321/445 (72.13%), Query Frame = 0

Query: 7   IKVVMALACM--AVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHLNNQV 66
           IK      C+  A ++P+L A I  +DE   ++A+EAK+ +   + P P NVT   N ++
Sbjct: 6   IKYSFLFLCITFATIIPSLMAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKL 65

Query: 67  HLALE-------GSGNSSRRSL--RKYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLG 126
              ++          NS+RR L  +KY GPC+ TNPID+CWRCDPNWA NRKKLA C +G
Sbjct: 66  RDTMKELKKVKGTHNNSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMG 125

Query: 127 FGRKTTGGKRGKIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLV 186
           FG K  GGK G+ Y V D SD D  +PKPGTLRHA IQK PLWIIF R M I+L +E+++
Sbjct: 126 FGSKAIGGKDGEFYVVTDNSD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIM 185

Query: 187 ASHKTIDARGKNIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVS 246
            S KTIDARG N+HI  GAG+++Q+  NVIIHGLHIH +V  +GG++RDA +H+GIRT S
Sbjct: 186 QSDKTIDARGVNVHITKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKS 245

Query: 247 DGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQ 306
           DGDGISIFG+S IW+DH+SM  C DGLIDA++GST IT+SN HFT HN+VMLFGASDS  
Sbjct: 246 DGDGISIFGASYIWIDHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSS 305

Query: 307 GDSIMQVTVAFNHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRF 366
            D +MQ+T+AFNHFGK LIQRMPRCRWG+ HVVNNDYTHW MYAIGGS HPTII QGNRF
Sbjct: 306 IDQVMQITLAFNHFGKRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRF 365

Query: 367 IAPPNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFVQSGNPS-KRRSYSRFDMIK 426
           IAPP+   +Q+T R Y  ESVW  WTWRSEG+L MNGAYF +SG+P    +    +D I 
Sbjct: 366 IAPPDIFKKQVTKREYNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDLYDGIS 425

Query: 427 AKPGTFVRRLTRFAGALNCYLGRPC 440
           A P   V  +TRFAG L C  G+PC
Sbjct: 426 AAPAEDVTWMTRFAGVLGCKPGKPC 449

BLAST of Tan0010705 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 3.5e-151
Identity = 254/440 (57.73%), Postives = 318/440 (72.27%), Query Frame = 0

Query: 16  MAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHLNNQVHLALEG--SGN 75
           +A++ P +R  +A FD Y  Q+  +A + ++ ++ P P+NVT H N  V++A++   S N
Sbjct: 20  LAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRN 79

Query: 76  SSRRSL---------RKYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKTTGGK 135
            +RR L          K +G CLA NPID CWRCD NWA NRKKLA CVLGFGR+TTGGK
Sbjct: 80  DTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGK 139

Query: 136 RGKIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASHKTIDAR 195
            G IY V D SDND++NPKPGTLRHA  +  PLWIIF RSMIIKL +EL++ S KTID R
Sbjct: 140 DGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGR 199

Query: 196 GKNIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGISIFG 255
           G  ++I  GAGL++QF  NVIIH +++ H+V  +GG+IRD+  H+G+RT SDGDGIS+FG
Sbjct: 200 GARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFG 259

Query: 256 SSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDSIMQVTV 315
           ++NIW+DH+SM+ C DG+IDAI GSTA+T+SN HFT H +VMLFGA D +  D  MQ+TV
Sbjct: 260 ATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITV 319

Query: 316 AFNHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAAR 375
           AFNHFGK L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTIISQGNRFIAPPN  A+
Sbjct: 320 AFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAK 379

Query: 376 QITNRNYATESVWRTWTWRSEGDLMMNGAYFVQSGNPSKRRSYSRFDM-----IKAKPGT 435
           QIT R Y     W++W W+SEGD  +NGAYFVQSG  +   S  +  +     I+ KPGT
Sbjct: 380 QITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGT 439

Query: 436 FVRRLTRFAGALNCYLGRPC 440
            VR+LT  AG L C LG  C
Sbjct: 440 MVRKLTMDAGVLGCKLGEAC 459

BLAST of Tan0010705 vs. NCBI nr
Match: XP_004147462.2 (probable pectate lyase 7 [Cucumis sativus] >KGN59593.1 hypothetical protein Csa_001450 [Cucumis sativus])

HSP 1 Score: 816.6 bits (2108), Expect = 1.0e-232
Identity = 381/441 (86.39%), Postives = 414/441 (93.88%), Query Frame = 0

Query: 1   MDGCRMIKVVMALACMAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHL 60
           M GC+ IKVVM L  MAV++PTLRAGIAEFD++LLQK++EAKQASLEAFHPDPMNVT+H 
Sbjct: 2   MTGCK-IKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF 61

Query: 61  NNQVHLALEG--SGNSSRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGR 120
           N  VHLALEG    NS+RRSL KYNGPCLATNPIDRCWRCDPNWAKNRKKLA CVLGFGR
Sbjct: 62  NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 121

Query: 121 KTTGGKRGKIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASH 180
           KTTGGK+G+IY V DPSDNDV+NP+PGTLR+ A+QK+PLWIIF RSMII+LSKEL++ SH
Sbjct: 122 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 181

Query: 181 KTIDARGKNIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGD 240
           KTIDARG N+HIAYGAGLS+QF+ NVIIHGL IHHV++T GG+IRD  NHVG+RTVSDGD
Sbjct: 182 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 241

Query: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDS 300
           GISIFGS+NIWLDHLSMSNCQDGLIDAIQGSTAIT+SN HFTHHNDVMLFGASDSYQGDS
Sbjct: 242 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 301

Query: 301 IMQVTVAFNHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGL+QRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 302 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 361

Query: 361 PNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPG 420
           PNPAARQITNRNYATESVW+TWTWRSEGDLMMNGAYFVQSGNPSKRR YSRFDMIKAKPG
Sbjct: 362 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPG 421

Query: 421 TFVRRLTRFAGALNCYLGRPC 440
           TFVRRLTRF+G+LNCY+GRPC
Sbjct: 422 TFVRRLTRFSGSLNCYVGRPC 441

BLAST of Tan0010705 vs. NCBI nr
Match: XP_038905811.1 (pectate lyase-like [Benincasa hispida])

HSP 1 Score: 815.8 bits (2106), Expect = 1.7e-232
Identity = 382/441 (86.62%), Postives = 412/441 (93.42%), Query Frame = 0

Query: 1   MDGCRMIKVVMALACMAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHL 60
           M GC  IKVVM L  MAV++PTLRAGIAE+D+YLLQKA+EAKQA+LE+FHPDPMNVT+H 
Sbjct: 1   MAGCCSIKVVMFLVSMAVLMPTLRAGIAEYDDYLLQKAEEAKQAALESFHPDPMNVTDHF 60

Query: 61  NNQVHLALEG--SGNSSRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGR 120
           N  VHLALEG    NS+RRSL+KYNGPC+ATNPIDRCWRCD NWA NRKKL SCVLGFGR
Sbjct: 61  NEHVHLALEGIEGSNSTRRSLKKYNGPCMATNPIDRCWRCDRNWAMNRKKLVSCVLGFGR 120

Query: 121 KTTGGKRGKIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASH 180
           KTTGG RG+ Y V DPSDNDV+NP+ GTLRHAAIQKRPLWIIFGRSMII+LSKELL+ SH
Sbjct: 121 KTTGGLRGRFYVVTDPSDNDVINPRRGTLRHAAIQKRPLWIIFGRSMIIRLSKELLITSH 180

Query: 181 KTIDARGKNIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGD 240
           KTIDARG N+HIAYGAGLS+QFS NVIIHGLHIHHVV+  GG+IRD+ +HVG+RTVSDGD
Sbjct: 181 KTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVVSARGGMIRDSADHVGLRTVSDGD 240

Query: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDS 300
           GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAIT+SN HFTHHNDVMLFGASDSYQGDS
Sbjct: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGL+QRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPG 420
           PNPAARQ+TNRNYATESVWRTWTWRSEGDLMMNGAYF+QSGNPSKRR YSRFDMIKAKPG
Sbjct: 361 PNPAARQVTNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420

Query: 421 TFVRRLTRFAGALNCYLGRPC 440
           TFVRRLTRF+G+LNCY+GRPC
Sbjct: 421 TFVRRLTRFSGSLNCYVGRPC 441

BLAST of Tan0010705 vs. NCBI nr
Match: XP_008443432.1 (PREDICTED: probable pectate lyase 19 [Cucumis melo])

HSP 1 Score: 813.5 bits (2100), Expect = 8.7e-232
Identity = 381/439 (86.79%), Postives = 411/439 (93.62%), Query Frame = 0

Query: 3   GCRMIKVVMALACMAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHLNN 62
           GC+ IK++M L  MAV++PTLRAGIAEFD+YLLQKA+EAKQASLEAFHPDPMNVT+H N 
Sbjct: 4   GCK-IKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHFNQ 63

Query: 63  QVHLALEG--SGNSSRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKT 122
            VHLALEG    NS+RRSL KY GPCLATNPIDRCWRCDPNWAKNRKKLA CVLGFGRKT
Sbjct: 64  HVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRKT 123

Query: 123 TGGKRGKIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASHKT 182
           TGGK+G+IY V DPSDNDVVNPKPGTLR+ A+QKRPLWIIF RSMII+LSKEL+V SHKT
Sbjct: 124 TGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSHKT 183

Query: 183 IDARGKNIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGI 242
           IDARG N+HIAYGAGLS+QF+ NVIIHGL IHHV++T GG+IRD  NHVG+RTVSDGDGI
Sbjct: 184 IDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDGI 243

Query: 243 SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDSIM 302
           SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAIT+SN HFTHHNDVMLFGASDSYQGDSIM
Sbjct: 244 SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIM 303

Query: 303 QVTVAFNHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN 362
           QVTVAFNHFGKGL+QRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN
Sbjct: 304 QVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN 363

Query: 363 PAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPGTF 422
           PAARQITNRNYA ESVW+TWTWRSEGDLMMNGAYF+QSGNPSKRR YSRFDMIKAKPGTF
Sbjct: 364 PAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPGTF 423

Query: 423 VRRLTRFAGALNCYLGRPC 440
           VRRLTRF+G+LNC++GRPC
Sbjct: 424 VRRLTRFSGSLNCFVGRPC 441

BLAST of Tan0010705 vs. NCBI nr
Match: XP_022152137.1 (probable pectate lyase 19 [Momordica charantia])

HSP 1 Score: 790.4 bits (2040), Expect = 7.9e-225
Identity = 366/433 (84.53%), Postives = 406/433 (93.76%), Query Frame = 0

Query: 7   IKVVMALACMAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHLNNQVHL 66
           ++ +M L  +AV+LPTLRAGIAE+DEYLLQKA+EAK ASLEAFH DPMNVT+H N QVHL
Sbjct: 6   VEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNLQVHL 65

Query: 67  ALEGSGNSSRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKTTGGKRG 126
           ALEGS NS+RRS++KYNGPC+ATNPIDRCWRCDPNWA NRKKLA+CVLGFGRKT GG +G
Sbjct: 66  ALEGS-NSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRKTRGGVKG 125

Query: 127 KIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASHKTIDARGK 186
           KIY V DPSD DVVNPKPGTLR+ A+QK+PLWIIFGRSMII LSKELLVASHKTIDARG 
Sbjct: 126 KIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHKTIDARGA 185

Query: 187 NIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGISIFGSS 246
           N+HIA+GAG+S+QF+ NVIIHGLHIHH++  +GG+IRD+ NHVG+RT SDGDG+SIFGS+
Sbjct: 186 NVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDGVSIFGST 245

Query: 247 NIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDSIMQVTVAF 306
           NIWLDHLSMSNC DGLIDAIQGSTAIT+SN HFTHHNDVMLFGASDSYQGDS+MQVTVAF
Sbjct: 246 NIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSVMQVTVAF 305

Query: 307 NHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAARQI 366
           NHFGKGL+QRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAA+QI
Sbjct: 306 NHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAAKQI 365

Query: 367 TNRNYATESVWRTWTWRSEGDLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPGTFVRRLTR 426
           TNRNYATE+VW+TWTWRSEGDLMMNGAYFVQSGNPSKRR YSR DMIKAKPGTFVRRLTR
Sbjct: 366 TNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGTFVRRLTR 425

Query: 427 FAGALNCYLGRPC 440
           F+G+LNC++GRPC
Sbjct: 426 FSGSLNCFVGRPC 437

BLAST of Tan0010705 vs. NCBI nr
Match: KAG7022233.1 (putative pectate lyase P59, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 787.7 bits (2033), Expect = 5.1e-224
Identity = 372/439 (84.74%), Postives = 403/439 (91.80%), Query Frame = 0

Query: 1   MDGCRMIKVVMALACMAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHL 60
           M GC    +++ L  MAV++PTLRAGIAE+D++LL+KA  A+Q + EA++PDPMNVT HL
Sbjct: 1   MAGCGFKVLMVLLLSMAVLMPTLRAGIAEYDDFLLKKAVAAEQVAQEAYNPDPMNVTNHL 60

Query: 61  NNQVHLALEGSGNSSRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKT 120
           N QVHLA EGS NS+RR L KY GPCLATNPIDRCWRC+  WAKNRKKLASCVLGFGRKT
Sbjct: 61  NLQVHLAFEGS-NSTRRGLAKYKGPCLATNPIDRCWRCNKKWAKNRKKLASCVLGFGRKT 120

Query: 121 TGGKRGKIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASHKT 180
           TGGKRG+ Y V DPSD + VNPKPGTLRHA IQKRPLWI FGRSMIIKLSKELL++SHKT
Sbjct: 121 TGGKRGRYYKVTDPSDTEFVNPKPGTLRHACIQKRPLWITFGRSMIIKLSKELLISSHKT 180

Query: 181 IDARGKNIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGI 240
           IDARG N+HIAYGAGLS+QFS NVIIHGLHIHH+VA +GG+IRDAYNHVG+RTVSDGDGI
Sbjct: 181 IDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHIVAANGGMIRDAYNHVGLRTVSDGDGI 240

Query: 241 SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDSIM 300
           SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSN HFTHHNDVMLFGASDSYQGDSIM
Sbjct: 241 SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNSHFTHHNDVMLFGASDSYQGDSIM 300

Query: 301 QVTVAFNHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN 360
           QVTVAFNHFGKGL+QRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN
Sbjct: 301 QVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN 360

Query: 361 PAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPGTF 420
           PAARQITNRNYA ESVW+TWTWRSEGDL+MNGAYFVQSG+PSKRR YSR DMIKAKPGT+
Sbjct: 361 PAARQITNRNYAPESVWKTWTWRSEGDLLMNGAYFVQSGSPSKRRPYSRLDMIKAKPGTY 420

Query: 421 VRRLTRFAGALNCYLGRPC 440
           VRRLTRFAG+LNCY GRPC
Sbjct: 421 VRRLTRFAGSLNCYEGRPC 438

BLAST of Tan0010705 vs. ExPASy TrEMBL
Match: A0A0A0LCU3 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_3G827350 PE=3 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 5.0e-233
Identity = 381/441 (86.39%), Postives = 414/441 (93.88%), Query Frame = 0

Query: 1   MDGCRMIKVVMALACMAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHL 60
           M GC+ IKVVM L  MAV++PTLRAGIAEFD++LLQK++EAKQASLEAFHPDPMNVT+H 
Sbjct: 2   MTGCK-IKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF 61

Query: 61  NNQVHLALEG--SGNSSRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGR 120
           N  VHLALEG    NS+RRSL KYNGPCLATNPIDRCWRCDPNWAKNRKKLA CVLGFGR
Sbjct: 62  NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 121

Query: 121 KTTGGKRGKIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASH 180
           KTTGGK+G+IY V DPSDNDV+NP+PGTLR+ A+QK+PLWIIF RSMII+LSKEL++ SH
Sbjct: 122 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 181

Query: 181 KTIDARGKNIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGD 240
           KTIDARG N+HIAYGAGLS+QF+ NVIIHGL IHHV++T GG+IRD  NHVG+RTVSDGD
Sbjct: 182 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 241

Query: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDS 300
           GISIFGS+NIWLDHLSMSNCQDGLIDAIQGSTAIT+SN HFTHHNDVMLFGASDSYQGDS
Sbjct: 242 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 301

Query: 301 IMQVTVAFNHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGL+QRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 302 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 361

Query: 361 PNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPG 420
           PNPAARQITNRNYATESVW+TWTWRSEGDLMMNGAYFVQSGNPSKRR YSRFDMIKAKPG
Sbjct: 362 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPG 421

Query: 421 TFVRRLTRFAGALNCYLGRPC 440
           TFVRRLTRF+G+LNCY+GRPC
Sbjct: 422 TFVRRLTRFSGSLNCYVGRPC 441

BLAST of Tan0010705 vs. ExPASy TrEMBL
Match: A0A1S3B8R7 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103487022 PE=3 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 4.2e-232
Identity = 381/439 (86.79%), Postives = 411/439 (93.62%), Query Frame = 0

Query: 3   GCRMIKVVMALACMAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHLNN 62
           GC+ IK++M L  MAV++PTLRAGIAEFD+YLLQKA+EAKQASLEAFHPDPMNVT+H N 
Sbjct: 4   GCK-IKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHFNQ 63

Query: 63  QVHLALEG--SGNSSRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKT 122
            VHLALEG    NS+RRSL KY GPCLATNPIDRCWRCDPNWAKNRKKLA CVLGFGRKT
Sbjct: 64  HVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRKT 123

Query: 123 TGGKRGKIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASHKT 182
           TGGK+G+IY V DPSDNDVVNPKPGTLR+ A+QKRPLWIIF RSMII+LSKEL+V SHKT
Sbjct: 124 TGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSHKT 183

Query: 183 IDARGKNIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGI 242
           IDARG N+HIAYGAGLS+QF+ NVIIHGL IHHV++T GG+IRD  NHVG+RTVSDGDGI
Sbjct: 184 IDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDGI 243

Query: 243 SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDSIM 302
           SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAIT+SN HFTHHNDVMLFGASDSYQGDSIM
Sbjct: 244 SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIM 303

Query: 303 QVTVAFNHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN 362
           QVTVAFNHFGKGL+QRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN
Sbjct: 304 QVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN 363

Query: 363 PAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPGTF 422
           PAARQITNRNYA ESVW+TWTWRSEGDLMMNGAYF+QSGNPSKRR YSRFDMIKAKPGTF
Sbjct: 364 PAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPGTF 423

Query: 423 VRRLTRFAGALNCYLGRPC 440
           VRRLTRF+G+LNC++GRPC
Sbjct: 424 VRRLTRFSGSLNCFVGRPC 441

BLAST of Tan0010705 vs. ExPASy TrEMBL
Match: A0A6J1DFD2 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111019925 PE=3 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 3.8e-225
Identity = 366/433 (84.53%), Postives = 406/433 (93.76%), Query Frame = 0

Query: 7   IKVVMALACMAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHLNNQVHL 66
           ++ +M L  +AV+LPTLRAGIAE+DEYLLQKA+EAK ASLEAFH DPMNVT+H N QVHL
Sbjct: 6   VEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFNLQVHL 65

Query: 67  ALEGSGNSSRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKTTGGKRG 126
           ALEGS NS+RRS++KYNGPC+ATNPIDRCWRCDPNWA NRKKLA+CVLGFGRKT GG +G
Sbjct: 66  ALEGS-NSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRKTRGGVKG 125

Query: 127 KIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASHKTIDARGK 186
           KIY V DPSD DVVNPKPGTLR+ A+QK+PLWIIFGRSMII LSKELLVASHKTIDARG 
Sbjct: 126 KIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHKTIDARGA 185

Query: 187 NIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGISIFGSS 246
           N+HIA+GAG+S+QF+ NVIIHGLHIHH++  +GG+IRD+ NHVG+RT SDGDG+SIFGS+
Sbjct: 186 NVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDGVSIFGST 245

Query: 247 NIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDSIMQVTVAF 306
           NIWLDHLSMSNC DGLIDAIQGSTAIT+SN HFTHHNDVMLFGASDSYQGDS+MQVTVAF
Sbjct: 246 NIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSVMQVTVAF 305

Query: 307 NHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAARQI 366
           NHFGKGL+QRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAA+QI
Sbjct: 306 NHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAAKQI 365

Query: 367 TNRNYATESVWRTWTWRSEGDLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPGTFVRRLTR 426
           TNRNYATE+VW+TWTWRSEGDLMMNGAYFVQSGNPSKRR YSR DMIKAKPGTFVRRLTR
Sbjct: 366 TNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGTFVRRLTR 425

Query: 427 FAGALNCYLGRPC 440
           F+G+LNC++GRPC
Sbjct: 426 FSGSLNCFVGRPC 437

BLAST of Tan0010705 vs. ExPASy TrEMBL
Match: A0A6J1ELF8 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111435488 PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 2.1e-223
Identity = 370/439 (84.28%), Postives = 403/439 (91.80%), Query Frame = 0

Query: 1   MDGCRMIKVVMALACMAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHL 60
           M GC    +++ L  MAV++PT+RAGIAE+D++LL+KA  A+Q + EA++PDPMNVT HL
Sbjct: 2   MAGCWFKVLMVLLLSMAVLMPTIRAGIAEYDDFLLKKAVAAEQVAQEAYNPDPMNVTNHL 61

Query: 61  NNQVHLALEGSGNSSRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKT 120
           N QVHLA EGS NS+RR L KY GPCLATNPIDRCWRC+  WAKNRKKLASCVLGFGRKT
Sbjct: 62  NLQVHLAFEGS-NSTRRGLAKYKGPCLATNPIDRCWRCNKKWAKNRKKLASCVLGFGRKT 121

Query: 121 TGGKRGKIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASHKT 180
           TGGKRG+ Y V DPSD + V+PKPGTLRHA IQKRPLWI FGRSMIIKLSKELL++SHKT
Sbjct: 122 TGGKRGRYYKVTDPSDTEFVDPKPGTLRHACIQKRPLWITFGRSMIIKLSKELLISSHKT 181

Query: 181 IDARGKNIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGI 240
           IDARG N+HIAYGAGLS+QFS NVIIHGLHIHH+VA +GG+IRDAYNHVG+RTVSDGDGI
Sbjct: 182 IDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHIVAANGGMIRDAYNHVGLRTVSDGDGI 241

Query: 241 SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDSIM 300
           SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSN HFTHHNDVMLFGASDSYQGDSIM
Sbjct: 242 SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNSHFTHHNDVMLFGASDSYQGDSIM 301

Query: 301 QVTVAFNHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN 360
           QVTVAFNHFGKGL+QRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN
Sbjct: 302 QVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN 361

Query: 361 PAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPGTF 420
           PAARQITNRNYA ESVW+TWTWRSEGDL+MNGAYFVQSG+PSKRR YSR DMIKAKPGT+
Sbjct: 362 PAARQITNRNYAPESVWKTWTWRSEGDLLMNGAYFVQSGSPSKRRPYSRLDMIKAKPGTY 421

Query: 421 VRRLTRFAGALNCYLGRPC 440
           VRRLTRFAG+LNCY GRPC
Sbjct: 422 VRRLTRFAGSLNCYEGRPC 439

BLAST of Tan0010705 vs. ExPASy TrEMBL
Match: A0A6J1I070 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111469675 PE=3 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 3.0e-222
Identity = 369/439 (84.05%), Postives = 401/439 (91.34%), Query Frame = 0

Query: 1   MDGCRMIKVVMALACMAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHL 60
           M GCR   +++ L  MAV++PTLRAGIAE+D++LL KA  A+Q + EA++PDPMNVT HL
Sbjct: 2   MAGCRFKVLMVLLLSMAVLMPTLRAGIAEYDDFLLNKAVVAQQVAQEAYNPDPMNVTNHL 61

Query: 61  NNQVHLALEGSGNSSRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKT 120
           N QVHLA EGS NS+RR L KY GPCLATNPIDRCWRC+  WAKNRKKLASCVLGFGRKT
Sbjct: 62  NLQVHLAFEGS-NSTRRGLAKYKGPCLATNPIDRCWRCNRKWAKNRKKLASCVLGFGRKT 121

Query: 121 TGGKRGKIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASHKT 180
           TGGKRG+ Y V DPSD + V+PKPGTLRHA IQKRPLWIIFGRSMIIKLSKELL++SHKT
Sbjct: 122 TGGKRGRYYKVTDPSDTEFVDPKPGTLRHACIQKRPLWIIFGRSMIIKLSKELLISSHKT 181

Query: 181 IDARGKNIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGI 240
           IDARG N+HIAYGAGLS+QFS NVIIHGLHIHH+V  +GG+IRDAYNHVG+RTVSDGDGI
Sbjct: 182 IDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHIVEANGGMIRDAYNHVGLRTVSDGDGI 241

Query: 241 SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDSIM 300
           SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSN HFTHHNDVMLFGASDSYQGDSIM
Sbjct: 242 SIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNSHFTHHNDVMLFGASDSYQGDSIM 301

Query: 301 QVTVAFNHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN 360
           QVTVAFNHFGKGL+QRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN
Sbjct: 302 QVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN 361

Query: 361 PAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPGTF 420
           PAARQITNRNYA ESVW+TWTWRSEGDL+MNGAYF+QSG+ SKRR YSR DMIKAKPGT+
Sbjct: 362 PAARQITNRNYAPESVWKTWTWRSEGDLLMNGAYFIQSGSSSKRRPYSRLDMIKAKPGTY 421

Query: 421 VRRLTRFAGALNCYLGRPC 440
           VRRLTRFAG+LNCY GR C
Sbjct: 422 VRRLTRFAGSLNCYEGRAC 439

BLAST of Tan0010705 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 572.0 bits (1473), Expect = 4.1e-163
Identity = 273/473 (57.72%), Postives = 346/473 (73.15%), Query Frame = 0

Query: 1   MDGCRMIKVVMALACMAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHL 60
           M+  R+ K++    C+AV++PT+R  I+E DEY  Q+ADEA++ +L+A+H DP  + +H 
Sbjct: 1   MEMVRLSKLMFTF-CIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHF 60

Query: 61  NNQVH-------------------------LALEGSGNSSRRSLR--------KYNGPCL 120
           + + +                           L  S NS+RRSLR        K  GPC 
Sbjct: 61  HERHYDNSTDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCT 120

Query: 121 ATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKTTGGKRGKIYTVIDPSDNDVVNPKPGTL 180
           A+NPID+CWRC  +WAK RKKL  CV GFG +TTGGKRG+IY V    D D+VNPKPGTL
Sbjct: 121 ASNPIDKCWRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTL 180

Query: 181 RHAAIQKRPLWIIFGRSMIIKLSKELLVASHKTIDARGKNIHIAYGAGLSVQFSTNVIIH 240
           RHA IQK PLWIIF   M I+L++ELL+ SHKTIDARG N+H+A+GAG+++QF  NV+IH
Sbjct: 181 RHAVIQKEPLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIH 240

Query: 241 GLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQ 300
           GLHIHH+  +SGG+IRD+ +H G+RT +DGDG+SI+GSSNIWLDH+SMS CQDGLIDAI 
Sbjct: 241 GLHIHHISESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIV 300

Query: 301 GSTAITVSNCHFTHHNDVMLFGASDSYQGDSIMQVTVAFNHFGKGLIQRMPRCRWGFFHV 360
           GST IT+SN HFTHHNDVML GA ++ + D  MQVTVA+NHFGKGL+QRMPR RWGF HV
Sbjct: 301 GSTGITISNSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHV 360

Query: 361 VNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPN-PAARQITNRNYATESVWRTWTWRSEG 420
           VNNDYTHW +YAIGGSQ PTI+S GNRFIAPP+ P  R++T R+YA+E  W+ W WRS+ 
Sbjct: 361 VNNDYTHWELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDK 420

Query: 421 DLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPGTFVRRLTRFAGALNCYLGRPC 440
           D+ MNGAYF QSGNP  + +++R  MIK K G  V +LT++AGAL+C +GR C
Sbjct: 421 DVFMNGAYFRQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

BLAST of Tan0010705 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 568.5 bits (1464), Expect = 4.5e-162
Identity = 273/476 (57.35%), Postives = 345/476 (72.48%), Query Frame = 0

Query: 1   MDGCRMIKVVMALACMAVMLPTLRAGIA-EFDEYLLQKADEAKQASLEAFHPDPMNVTEH 60
           M+  R+ K+V  + C+A ++PT+RA +A E DEY + KA+EA++ +L A+HPDP  + +H
Sbjct: 1   METARLFKLVCVI-CIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDH 60

Query: 61  LNNQ-----------------------VHLALEGSGNSSRRSL------------RKYNG 120
            + +                       V   +    NS+RRSL             K  G
Sbjct: 61  FHERHYDNSTDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTG 120

Query: 121 PCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKTTGGKRGKIYTVIDPSDNDVVNPKP 180
           PC A+NPID+CWRC P+WA+ RKKL  CV GFG +TTGGKRG+IY V  P D+D+VNP+P
Sbjct: 121 PCTASNPIDKCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRP 180

Query: 181 GTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASHKTIDARGKNIHIAYGAGLSVQFSTNV 240
           GTLRHA IQK PLWI+F   M I+LS+EL++ S KTIDARG N+HIAYGAG+++Q+  N+
Sbjct: 181 GTLRHAVIQKEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNI 240

Query: 241 IIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGISIFGSSNIWLDHLSMSNCQDGLID 300
           IIHGLH+HH+V +SGG+IRD+ NH G R  +DGDGISIFG++NIWLDH+SMS CQDGLID
Sbjct: 241 IIHGLHVHHIVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLID 300

Query: 301 AIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDSIMQVTVAFNHFGKGLIQRMPRCRWGF 360
           AI GSTAIT+SN HFTHHNDVML GA ++   D  MQVTVA+NHFGKGL+QRMPR RWGF
Sbjct: 301 AIMGSTAITISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGF 360

Query: 361 FHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPA-ARQITNRNYATESVWRTWTWR 420
            HVVNNDYTHW +YAIGGSQ PTI+S GNRFIAPP+    R++T R+YA+ES W+ W WR
Sbjct: 361 VHVVNNDYTHWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWR 420

Query: 421 SEGDLMMNGAYFVQSGNPSKRRSYSRFDMIKAKPGTFVRRLTRFAGALNCYLGRPC 440
           SE D+ MN AYF QSGNP  + S+SR  MIK K G  V +LT++AGAL+C +G+ C
Sbjct: 421 SEKDVFMNNAYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of Tan0010705 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 536.2 bits (1380), Expect = 2.5e-152
Identity = 254/440 (57.73%), Postives = 318/440 (72.27%), Query Frame = 0

Query: 16  MAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHLNNQVHLALEG--SGN 75
           +A++ P +R  +A FD Y  Q+  +A + ++ ++ P P+NVT H N  V++A++   S N
Sbjct: 20  LAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRN 79

Query: 76  SSRRSL---------RKYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGRKTTGGK 135
            +RR L          K +G CLA NPID CWRCD NWA NRKKLA CVLGFGR+TTGGK
Sbjct: 80  DTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGK 139

Query: 136 RGKIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASHKTIDAR 195
            G IY V D SDND++NPKPGTLRHA  +  PLWIIF RSMIIKL +EL++ S KTID R
Sbjct: 140 DGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGR 199

Query: 196 GKNIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGDGISIFG 255
           G  ++I  GAGL++QF  NVIIH +++ H+V  +GG+IRD+  H+G+RT SDGDGIS+FG
Sbjct: 200 GARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFG 259

Query: 256 SSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDSIMQVTV 315
           ++NIW+DH+SM+ C DG+IDAI GSTA+T+SN HFT H +VMLFGA D +  D  MQ+TV
Sbjct: 260 ATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITV 319

Query: 316 AFNHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAAR 375
           AFNHFGK L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTIISQGNRFIAPPN  A+
Sbjct: 320 AFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAK 379

Query: 376 QITNRNYATESVWRTWTWRSEGDLMMNGAYFVQSGNPSKRRSYSRFDM-----IKAKPGT 435
           QIT R Y     W++W W+SEGD  +NGAYFVQSG  +   S  +  +     I+ KPGT
Sbjct: 380 QITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGT 439

Query: 436 FVRRLTRFAGALNCYLGRPC 440
            VR+LT  AG L C LG  C
Sbjct: 440 MVRKLTMDAGVLGCKLGEAC 459

BLAST of Tan0010705 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 507.7 bits (1306), Expect = 9.5e-144
Identity = 248/446 (55.61%), Postives = 317/446 (71.08%), Query Frame = 0

Query: 10  VMALACMAVMLPTLRAGIAEFDEYLLQKADEAKQASLEAFHPDPMNVTEHLNNQVHLALE 69
           V     + V++P+++A IAE+DEY  Q+   A + +LE++ P+P NVT+H N    LA+E
Sbjct: 10  VFVALSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHFNYHAALAME 69

Query: 70  GSG--NSSRRSLR---------KYNGPCLATNPIDRCWRCDPNWAKNRKKLASCVLGFGR 129
            +G  N +RR LR         +  G   + N ID+CWR D NW KNRKKLA CVLGFGR
Sbjct: 70  TTGIVNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADCVLGFGR 129

Query: 130 KTTGGKRGKIYTVIDPSDNDVVNPKPGTLRHAAIQKRPLWIIFGRSMIIKLSKELLVASH 189
           KTTGGK G IY V DPSDND++ PKPGT+RHA  + RPLWIIF RSMIIKL +EL++ + 
Sbjct: 130 KTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQELIITND 189

Query: 190 KTIDARGKNIHIAYGAGLSVQFSTNVIIHGLHIHHVVATSGGIIRDAYNHVGIRTVSDGD 249
           KTID RG  I+I  GAGL++QF  NVIIH +HI  +   +GG+I D+  H G+RTVSDGD
Sbjct: 190 KTIDGRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLRTVSDGD 249

Query: 250 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSNCHFTHHNDVMLFGASDSYQGDS 309
           GI+IFG++N+W+DH+SM++C DG+IDAI GSTAIT+SN HFT H++VMLFG ++    D 
Sbjct: 250 GINIFGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTNKDVIDK 309

Query: 310 IMQVTVAFNHFGKGLIQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 369
            MQ+TVAFNHFGK L QRMPR R+G  HVVNNDYTHW MYAIGG+ +PTIISQGNRFIAP
Sbjct: 310 KMQITVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAP 369

Query: 370 PNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFVQSG-----NPSKRRSYSRFDMI 429
           P   ++Q+T R Y     W++W W+SE D  +NGAYFVQSG     + + +    R   I
Sbjct: 370 PIEDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRKFAI 429

Query: 430 KAKPGTFVRRLTRFAGALNCYLGRPC 440
           + +PGT VRRLT+ AG L C  G+ C
Sbjct: 430 RPQPGTKVRRLTKDAGTLGCKPGKSC 455

BLAST of Tan0010705 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 478.8 bits (1231), Expect = 4.7e-135
Identity = 228/403 (56.58%), Postives = 286/403 (70.97%), Query Frame = 0

Query: 37  KADEAKQASLEAFHPDPMNVTEHLNNQVHLALEGSGNSSRRSLRKYNGPCLATNPIDRCW 96
           + D   Q    A   DP  V + +   V ++ +    ++RR L  ++  C   NPID CW
Sbjct: 73  RLDGEPQQQQHAVADDPDMVADEVAKLVQMSEQ--NRTARRKLGFFS--CGTGNPIDDCW 132

Query: 97  RCDPNWAKNRKKLASCVLGFGRKTTGGKRGKIYTVIDPSDNDVVNPKPGTLRHAAIQKRP 156
           RCD NW KNRK+LA C +GFGR   GG+ G+ Y V DP+D DVVNPKPGTLRHA IQ+ P
Sbjct: 133 RCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEP 192

Query: 157 LWIIFGRSMIIKLSKELLVASHKTIDARGKNIHIAYGAGLSVQFSTNVIIHGLHIHHVVA 216
           LWI+F R M+I+L +EL++ S KTIDARG N+HIA GA +++QF TNVIIHGLHIH    
Sbjct: 193 LWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKP 252

Query: 217 TSGGIIRDAYNHVGIRTVSDGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITVSN 276
           T   ++R + +H G RT++DGD +SIFGSS+IW+DH S+S+C DGL+DA+ GSTAITVSN
Sbjct: 253 TGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSN 312

Query: 277 CHFTHHNDVMLFGASDSYQGDSIMQVTVAFNHFGKGLIQRMPRCRWGFFHVVNNDYTHWL 336
            HFTHHN+VML G SDSY  D +MQVT+A+NHFG+GL+QRMPRCR G+FHVVNNDYTHW 
Sbjct: 313 NHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWE 372

Query: 337 MYAIGGSQHPTIISQGNRFIAPPNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFV 396
           MYAIGGS  PTI SQGNR+ AP +  A+++T R     S W+ W WRSEGDL++NGA+F 
Sbjct: 373 MYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFR 432

Query: 397 QSGNPSKRRSYSRFDMIKAKPGTFVRRLTRFAGALNCYLGRPC 440
            SG      SY R   + AKP + V  +T  AGAL C  GRPC
Sbjct: 433 PSG-AGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LFP54.4e-16257.93Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1[more]
Q9SRH46.4e-16157.35Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1[more]
P409733.0e-15863.70Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1[more]
P157223.7e-15358.88Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1[more]
Q9M9S23.5e-15157.73Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004147462.21.0e-23286.39probable pectate lyase 7 [Cucumis sativus] >KGN59593.1 hypothetical protein Csa_... [more]
XP_038905811.11.7e-23286.62pectate lyase-like [Benincasa hispida][more]
XP_008443432.18.7e-23286.79PREDICTED: probable pectate lyase 19 [Cucumis melo][more]
XP_022152137.17.9e-22584.53probable pectate lyase 19 [Momordica charantia][more]
KAG7022233.15.1e-22484.74putative pectate lyase P59, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A0A0LCU35.0e-23386.39Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_3G827350 PE=3 SV=1[more]
A0A1S3B8R74.2e-23286.79Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103487022 PE=3 SV=1[more]
A0A6J1DFD23.8e-22584.53Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111019925 PE=3 SV=1[more]
A0A6J1ELF82.1e-22384.28Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111435488 PE=3 SV=1[more]
A0A6J1I0703.0e-22284.05Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111469675 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G15110.14.1e-16357.72Pectate lyase family protein [more]
AT3G01270.14.5e-16257.35Pectate lyase family protein [more]
AT1G14420.12.5e-15257.73Pectate lyase family protein [more]
AT2G02720.19.5e-14455.61Pectate lyase family protein [more]
AT4G13710.14.7e-13556.58Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 316..335
score: 78.1
coord: 380..404
score: 53.71
coord: 338..357
score: 79.52
coord: 172..188
score: 55.74
coord: 136..161
score: 69.23
coord: 112..129
score: 60.05
coord: 410..433
score: 46.43
coord: 236..257
score: 63.42
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 164..361
e-value: 1.4E-96
score: 336.9
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 195..355
e-value: 3.4E-18
score: 66.0
IPR007524Pectate lyase, N-terminalPFAMPF04431Pec_lyase_Ncoord: 27..79
e-value: 5.4E-18
score: 65.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 89..432
e-value: 2.2E-112
score: 377.5
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 7..439
NoneNo IPR availablePANTHERPTHR31683:SF146PECTATE LYASEcoord: 7..439
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 90..430

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0010705.1Tan0010705.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity