Tan0010655 (gene) Snake gourd v1

Overview
NameTan0010655
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionAlpha-galactosidase
LocationLG04: 3771945 .. 3777115 (+)
RNA-Seq ExpressionTan0010655
SyntenyTan0010655
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATTCGGGGTAATTCGATGGAATTATTGGGGTACTGCCAAGTTTGCTGTGTTCTTTAATTATGGTGGAACCACCATTGATGGCACTTTCTCCATAATTGTTTGTTGTGCAGTGGAGAAGCATTACCAAATCCTGACGAATTCAATGCTCTGAGGTAAAGAATCAACCAGTTCTGATCTAAAACTTACTCTGTAAATGGTACATTTACCATTAATGCTCAATCACAGGCTACAGCCGTCCGCTATGGCTTTTCCCTCGCTCCCGCTTTCATCTCCAGCTTCGATTATGGTTCTCTTCTACTCCGTCCTTTTCTGGATGTTCATGTTAGGTAGCATTACCGACGGCAATGGCGGTCCGGTGGCCGGAGCGACTCGATCGAGACCTCTGCGGTTTGCCACTGAGTTTGATTCTGATTCCACCAGGAGAGTTCTCCTCAATAATGGCCTCGCTCTAACTCCTCCGATGGGGTAAGTCTTACGCTTCGATCATGAGTTTACTCTCTTTCTCACGAGGATCGATCAGATTGAATGTAATGTAGTCTCTCCTCAATAATCGATCGATCATGATTTTTCTAATGTTTTGCTTCCGGTTTGTTTTTTTTTTTTTTTTTTGATAAGCACAGATGGAATAGCTGGAACCATTTTCAATGCAATTTAAACGAGAATTTAATCAAGGAAACAGGTGATATTTTGATTTGAAGGCGTCTTTAATTTCTGTTTGGATTTTGTGTACAGTGACTGAGATACACATCCTATTTCTTTTGCTAAGCGGATGCAATGGTATCCAGCGCCCTTGCTGCGTTAGGATATGAGTATATCAATTTAGGTGATCATTATATTTCTTTCTTTTTACATTTCTTTGGAATTTTACCCTTTCTCTTTTTGATATTAATATGATCTTATTTCTATTTGAGTTAGATGATTGTTGGGCTGAACTGAATAGAGATTCTAAGGTAAATTTCAATGTCCAATTTCTTTTCTCGGGTGTCTATGAAAATCTCAATATTTGCATTATATTATGAAGTTTATTGTTGAAGATTCCATCGATTTTTTCAGGGTAATTTGGTTGCTAAAGCTTCAACATTCCCTTCTGGTATTAAGGCCCTAGCAGATTATGTTCATAGAAAAGGGCTCAAGCTCGGAATTTACTCTGATGCTGGGTAAATCTATACACTCATCACATCAGCTTAAACAACTTCCTCGTTCATTTTCATTGTCATAGTTCATCGAGATGATGCTGATAGTTTTATTTGCTGATTTTCTCCCTACTTTTGGCTTGCAAATTCCATTATCAACCCTGTTTCACTAAGATCTTCTTTACCCAAGTTAATATAATTTCTTCAGGATCCAGACTTGCAGTAAAAAGATGCCAGGTTCCCTCGGTCACGAAGAACAAGATGCAAAAACTTTTGCCTCATGGGTATTTTTTCTGCTTTACACTCTTAAATGTTAACTCCTTTATTTATTGTTTAGATCGCTATAAATTATCAGGAATAGTTGATATATAATACAATTGTGTTAAGAGAACTCATTAAGTGCAACCATATATTTCTGTTACTAACCATAGTTGAGTTAGTTGTACATATGAGAGTTTATAGTTGAGTCTGTTATTTGATTAAATATCATTTTCTTTATAAATGATTATGTACTCTCTTGATTAATGAATGAGAAAGTACAACAACCATTCATTATAGTTGAGTCTGTTGTTTGATTAAATATCTTTTAATATTTTGTTAAATTGTATAGGGGATCGATTACTTGAAGTATGATAACTGTGAAAATACTGGTACAAGCCCCAAAGAAAGGTAGGATCAACTTTCTTTGTTTAATTGTCAAAATTTTCAAGTAACTGCAACCATCCGTAGGACACAGATACCCGAAGATGACTAAAGCTTTACAACAATCTGGGAGACCCATACTTTTTTCTTTATGCGAATGGTTAGCAATTCTTAGAAGACAGACTTGCTCTATTTTACTCTAATGTTTCTCCATTCTATAGTACAAACAGAGTTCATTTCTTAAGTTTTTCTTCATTTAGGGGACAAGAAGATCCAGCAACTTGGGCAGTAAATGTAGGAAATAGTTGGAGAACGACATCAGATATTCAAGATAACTGGAATAGGTAGTGAGCATAAATTTTGTCAACATTTCAGGAAACATGCAACATTTTCAAAAAATTTCTCTACACTATTTGATTAGTTTCTTTTCCCAGTATCTATGAATATGCCTGCATTGAAGTATATGATATTTCATGTCCAAAGTACTTTCTTTAGCAAGAATCTGATGCAGCTACTTAATGATACAGCATGACAACCATTGCTGATCAAAATGACAAATGGGCATCTTATGCTAAACCTGGAGGATGGAATGGTAATTTCATAATTATAGTTTGATATGAAAATTAAACACGTACCCTTGTGTATAGAGAAATATAGGCTGGTTAAGCTTGACGGTTGGCCTTGGCCCCTTTTCATATCTCTCAGAACTAATTAAAGATCGATAATGATTGAAGAAGCGAATCCAATGCTGATTCTGAACAGAGGACTAGACGATCTCTACTTCTGCCATTAGCGACCAACTGTTTTAACTCAACCTTAAACAATCTTCTATTGAAAGGAAAAGTAAAATATAAACTTCTGAGTATTCATAGTTATTATCAAATAGCTTCTTTTTATGATTATGATTAAATAGCTTTTTCTATGCATTCAAAATTCTTTCCCCATGGCCAATCTCTATGGAAATTGTACAACAATCTCAGAAATGTTAAAGAAAATAAGCTTAGTTTAAATTTTCATTTGTTCAGATCCTGACATGCTTGAAGTTGGGAATGGAGGGATGACAACAGCAGAATACCGTTCTCATTTTAGCATTTGGGCACTGGCAAAAGTAAGTTCCATTAAAACTTTTCCAGAATCTACTTTTTAGTTTAGATTGGTAATTTATCGGATCGTATTAAGACATATTCGGTATTCAAGTTCATTAAACGACTTAGCCATTTCAAATAACTATATTAAGATAGGGTTGAATATCTTTGCTTGATTTGTGGGTTGAGTATATAATTTTGCGTGCTTCATAAACTACCTTATCAATTTATTATCGATTCCTTTCTGTTCTTCAAATGTGAAGGCTCCATTGCTGATTGGATGTGACATCAGATCAATGGACAACATCACCTTGAAATTGTTAAGTAATAAGGAGGTTATTGCAGTTAATCAGGGTAAGTAAAAGCCAAGCTGTTTCTGCTAATTCATAGAGAACTATGAAATAGATAACATAGAAATAATTCTCTCAATAAAATGGAAGATTAATCATTAACCAAATATGACATTATGCTCCATCTAAAAGTTTTTTATCCAAGTAGATAAGCTTGGAGTTCAAGGGAAGAAGGTCTATAAATTTGGAGATCTTGAGGTAATTTTTCATCATCTAACACATCCCAAAAAAAAAAACAAAAGGATAGAAGAAAAAAAAAAGGAAATTTTTTACTTTACTCTGAAATTTTCTACACAAAAAAAAAAAAACAATGCAGGTTTGGGCAGGGCCACTGAGTGGTAAAAGAGTTGCTGTTGTTCTATGGAACAGAGGCTTCTCGAGAGTCAATATCACTGCATCTTGGTCAGCTATAGGTTTGAGTCCATCAACTACTGTTACTGCTAGAGACTTATGGGAGGTAAGATATGAAGCGTGTAATTAAAAGTACTTTTCAAAGTTTTCATGACATTAGGGGGTTGCTGGTGAAAAAAAAGTGTTTTTCAAAAATGCATTTTTTTAAAACATGTTTAATAAAAACAGTTTAAAATTAGAAAACTCAATTGAGATAATCATTTTAGTTTTTGAAAATTAAACCTAGAAATACTACTCCTATTTAAGGATTTGAGTGTCTTGTTATCTACTTTTTACCTATATTTTCAAAAACCAAGCTATGTTTTGAAAACTAAATAAAGTAGTTTTTAAAAACTTGTTTTTGTTTTTAGAATTTAGCTAAGAATCCGAATGTTTTCCTTAAGGGACATGAAAACCATAGTAGAGAAGTTGGGAGAAAACAAGCTTAATTTTCAAAAATCAAATAGTTATCAAATGGAGTCTCATATCTTTGGTTACATTTTCTAAAATGTGTTTTTATACACAAGTTTAATTGGTTTCTCTACCATTAAAATACTTTTATTAATGCCAAATTACCAAAAATAACGACTACTAAAGATTATGAACTAATAAGTTCAAATTTGTATTTAAAATACTATGATCAGCCGACTGCAATTAGAGGAGTTGTCGGAAATGGTTGCCGGTGGTGGTCACCGGAGATTATCGCTGGTGATGGGTAGCAATATTGTGAGGATATTTTGGTCACTTCCATATATCATGAAAATTTGGGAGAGATTCTAAATTAAACACTTACAAATCCTTTTTCAAAAAGCAGAGTGTTAAGCCGAAAATAACTGATTTCTCACTCATTTGTTAAAAAATCATTTAGAATAGTGGTTATATCCTACCATTTTTATTAAAATGACTTTTTTTTTTTTGAAAAATCAAACCAATGGACCTTAGATTACAAGGTAAGAAGTACTTTTAAAAACTCTAATAATTAATAGTTGAAATGAATTATTATGAAATAAGCACATTATTAGTACTTTCTTAAAAAAATCGTTTCCCTAAAAAGTACTATTCTCAAAAGTTCTTCTAGACTCACTCGAAGAAATGTGCATTATTTCTATGTATGTACATTGCAAAAATTTTCTGACTGATTATTCATTCCTACAATAGCACTCCAGTCAGATGGTTCAAAATCAACTTACTGCTCAAGTAGACTCTCATGATTGTAAGATGTATGTTCTCACACCCCATTAAAGCCCAATGTGAAAAATTGCCAATGTTCTTGTGAATGATTATTGTCTTCACTTCATCTACTTGTCCTTCCACTTTATGCTGTGATTTCTGGGATTTTACATGTTTATTTGTATAAGAAGTTGCCTTTCTTTCTTCTCAAGGTGGATGAACAAATGCAACCAGATTCTTGGGTTTCTTGTTTTTCCTTTTTCTTTTTGTCTAATTTATTAGGAACTTTATAGTTGTTCAATGTTGAGAATAACATACTTTATATGAATAATTTCACACTTTTTTCTCACTAATGTATTTCCCTTTATACACACCACTTTCATTAATCCTTTTCTAATC

mRNA sequence

TTATTCGGGGTAATTCGATGGAATTATTGGGGTACTGCCAAGTTTGCTGTGTTCTTTAATTATGGTGGAACCACCATTGATGGCACTTTCTCCATAATTGTTTGTTGTGCAGTGGAGAAGCATTACCAAATCCTGACGAATTCAATGCTCTGAGGCTACAGCCGTCCGCTATGGCTTTTCCCTCGCTCCCGCTTTCATCTCCAGCTTCGATTATGGTTCTCTTCTACTCCGTCCTTTTCTGGATGTTCATGTTAGGTAGCATTACCGACGGCAATGGCGGTCCGGTGGCCGGAGCGACTCGATCGAGACCTCTGCGGTTTGCCACTGAGTTTGATTCTGATTCCACCAGGAGAGTTCTCCTCAATAATGGCCTCGCTCTAACTCCTCCGATGGGATGGAATAGCTGGAACCATTTTCAATGCAATTTAAACGAGAATTTAATCAAGGAAACAGCGGATGCAATGGTATCCAGCGCCCTTGCTGCGTTAGGATATGAGTATATCAATTTAGATGATTGTTGGGCTGAACTGAATAGAGATTCTAAGGGTAATTTGGTTGCTAAAGCTTCAACATTCCCTTCTGGTATTAAGGCCCTAGCAGATTATGTTCATAGAAAAGGGCTCAAGCTCGGAATTTACTCTGATGCTGGGATCCAGACTTGCAGTAAAAAGATGCCAGGTTCCCTCGGTCACGAAGAACAAGATGCAAAAACTTTTGCCTCATGGGGGATCGATTACTTGAAGTATGATAACTGTGAAAATACTGGTACAAGCCCCAAAGAAAGATACCCGAAGATGACTAAAGCTTTACAACAATCTGGGAGACCCATACTTTTTTCTTTATGCGAATGGGGACAAGAAGATCCAGCAACTTGGGCAGTAAATGTAGGAAATAGTTGGAGAACGACATCAGATATTCAAGATAACTGGAATAGCATGACAACCATTGCTGATCAAAATGACAAATGGGCATCTTATGCTAAACCTGGAGGATGGAATGATCCTGACATGCTTGAAGTTGGGAATGGAGGGATGACAACAGCAGAATACCGTTCTCATTTTAGCATTTGGGCACTGGCAAAAGCTCCATTGCTGATTGGATGTGACATCAGATCAATGGACAACATCACCTTGAAATTGTTAAGTAATAAGGAGGTTATTGCAGTTAATCAGGATAAGCTTGGAGTTCAAGGGAAGAAGGTCTATAAATTTGGAGATCTTGAGGTTTGGGCAGGGCCACTGAGTGGTAAAAGAGTTGCTGTTGTTCTATGGAACAGAGGCTTCTCGAGAGTCAATATCACTGCATCTTGGTCAGCTATAGGTTTGAGTCCATCAACTACTGTTACTGCTAGAGACTTATGGGAGCACTCCAGTCAGATGGTTCAAAATCAACTTACTGCTCAAGTAGACTCTCATGATTGTAAGATGTATGTTCTCACACCCCATTAAAGCCCAATGTGAAAAATTGCCAATGTTCTTGTGAATGATTATTGTCTTCACTTCATCTACTTGTCCTTCCACTTTATGCTGTGATTTCTGGGATTTTACATGTTTATTTGTATAAGAAGTTGCCTTTCTTTCTTCTCAAGGTGGATGAACAAATGCAACCAGATTCTTGGGTTTCTTGTTTTTCCTTTTTCTTTTTGTCTAATTTATTAGGAACTTTATAGTTGTTCAATGTTGAGAATAACATACTTTATATGAATAATTTCACACTTTTTTCTCACTAATGTATTTCCCTTTATACACACCACTTTCATTAATCCTTTTCTAATC

Coding sequence (CDS)

ATGGCTTTTCCCTCGCTCCCGCTTTCATCTCCAGCTTCGATTATGGTTCTCTTCTACTCCGTCCTTTTCTGGATGTTCATGTTAGGTAGCATTACCGACGGCAATGGCGGTCCGGTGGCCGGAGCGACTCGATCGAGACCTCTGCGGTTTGCCACTGAGTTTGATTCTGATTCCACCAGGAGAGTTCTCCTCAATAATGGCCTCGCTCTAACTCCTCCGATGGGATGGAATAGCTGGAACCATTTTCAATGCAATTTAAACGAGAATTTAATCAAGGAAACAGCGGATGCAATGGTATCCAGCGCCCTTGCTGCGTTAGGATATGAGTATATCAATTTAGATGATTGTTGGGCTGAACTGAATAGAGATTCTAAGGGTAATTTGGTTGCTAAAGCTTCAACATTCCCTTCTGGTATTAAGGCCCTAGCAGATTATGTTCATAGAAAAGGGCTCAAGCTCGGAATTTACTCTGATGCTGGGATCCAGACTTGCAGTAAAAAGATGCCAGGTTCCCTCGGTCACGAAGAACAAGATGCAAAAACTTTTGCCTCATGGGGGATCGATTACTTGAAGTATGATAACTGTGAAAATACTGGTACAAGCCCCAAAGAAAGATACCCGAAGATGACTAAAGCTTTACAACAATCTGGGAGACCCATACTTTTTTCTTTATGCGAATGGGGACAAGAAGATCCAGCAACTTGGGCAGTAAATGTAGGAAATAGTTGGAGAACGACATCAGATATTCAAGATAACTGGAATAGCATGACAACCATTGCTGATCAAAATGACAAATGGGCATCTTATGCTAAACCTGGAGGATGGAATGATCCTGACATGCTTGAAGTTGGGAATGGAGGGATGACAACAGCAGAATACCGTTCTCATTTTAGCATTTGGGCACTGGCAAAAGCTCCATTGCTGATTGGATGTGACATCAGATCAATGGACAACATCACCTTGAAATTGTTAAGTAATAAGGAGGTTATTGCAGTTAATCAGGATAAGCTTGGAGTTCAAGGGAAGAAGGTCTATAAATTTGGAGATCTTGAGGTTTGGGCAGGGCCACTGAGTGGTAAAAGAGTTGCTGTTGTTCTATGGAACAGAGGCTTCTCGAGAGTCAATATCACTGCATCTTGGTCAGCTATAGGTTTGAGTCCATCAACTACTGTTACTGCTAGAGACTTATGGGAGCACTCCAGTCAGATGGTTCAAAATCAACTTACTGCTCAAGTAGACTCTCATGATTGTAAGATGTATGTTCTCACACCCCATTAA

Protein sequence

MAFPSLPLSSPASIMVLFYSVLFWMFMLGSITDGNGGPVAGATRSRPLRFATEFDSDSTRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAELNRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAGPLSGKRVAVVLWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHSSQMVQNQLTAQVDSHDCKMYVLTPH
Homology
BLAST of Tan0010655 vs. ExPASy Swiss-Prot
Match: Q8RX86 (Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 9.5e-178
Identity = 290/364 (79.67%), Postives = 321/364 (88.19%), Query Frame = 0

Query: 61  RVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAEL 120
           R+L+NNGLAL+P MGWNSWNHFQCN+NE LIK+TADAMVSS L+A+GY+YIN+DDCW EL
Sbjct: 29  RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGEL 88

Query: 121 NRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAK 180
            RDS+G+LVAKASTFPSGIKAL+DYVH KGLKLGIYSDAG  TCS+ MPGSLGHEEQDAK
Sbjct: 89  KRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAK 148

Query: 181 TFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVG 240
           TFASWGIDYLKYDNCENTGTSP+ERYPKM+KAL  SGR I FSLCEWGQEDPATWA ++G
Sbjct: 149 TFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG 208

Query: 241 NSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIW 300
           NSWRTT DIQDNW SMT IADQND+WASYA+PG WNDPDMLEVGNGGMT  EY SHFSIW
Sbjct: 209 NSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIW 268

Query: 301 ALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAGPLSGK 360
           ALAKAPLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV K GDLEVWAGPLS K
Sbjct: 269 ALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKK 328

Query: 361 RVAVVLWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHSS-QMVQNQLTAQVDSHDCKM 420
           RVAV+LWNRG +  NITA W+ IGL+ S  V ARDLWEHS+   V+ QL+A V+ H CKM
Sbjct: 329 RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSALVEPHACKM 388

Query: 421 YVLT 424
           Y LT
Sbjct: 389 YTLT 392

BLAST of Tan0010655 vs. ExPASy Swiss-Prot
Match: Q42656 (Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 3.6e-177
Identity = 294/369 (79.67%), Postives = 315/369 (85.37%), Query Frame = 0

Query: 57  DSTRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDC 116
           D TRR LL NGL LTPPMGWNSWNHF+CNL+E LI+ETADAMVS  LAALGY+YINLDDC
Sbjct: 9   DYTRRSLLANGLGLTPPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDDC 68

Query: 117 WAELNRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEE 176
           WAELNRDS+GNLV K STFPSGIKALADYVH KGLKLGIYSDAG QTCSK MPGSLGHEE
Sbjct: 69  WAELNRDSQGNLVPKGSTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEE 128

Query: 177 QDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWA 236
           QDAKTFASWG+DYLKYDNC N   SPKERYP M+KAL  SGR I FSLCEWG+EDPATWA
Sbjct: 129 QDAKTFASWGVDYLKYDNCNNNNISPKERYPIMSKALLNSGRSIFFSLCEWGEEDPATWA 188

Query: 237 VNVGNSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSH 296
             VGNSWRTT DI D+W+SMT+ AD NDKWASYA PGGWNDPDMLEVGNGGMTT EYRSH
Sbjct: 189 KEVGNSWRTTGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGGMTTTEYRSH 248

Query: 297 FSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAGP 356
           FSIWALAKAPLLIGCDIRSMD  T +LLSN EVIAVNQDKLGVQG KV  +GDLEVWAGP
Sbjct: 249 FSIWALAKAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGNKVKTYGDLEVWAGP 308

Query: 357 LSGKRVAVVLWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHSSQ-MVQNQLTAQVDSH 416
           LSGKRVAV LWNRG S   ITA WS +GL  +  V ARDLW HS++  V+ Q++A VD+H
Sbjct: 309 LSGKRVAVALWNRGSSTATITAYWSDVGLPSTAVVNARDLWAHSTEKSVKGQISAAVDAH 368

Query: 417 DCKMYVLTP 425
           D KMYVLTP
Sbjct: 369 DSKMYVLTP 377

BLAST of Tan0010655 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 1.0e-176
Identity = 287/389 (73.78%), Postives = 324/389 (83.29%), Query Frame = 0

Query: 37  GPVAGATRSRPLRFATEFDSDSTRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETAD 96
           G   G    +  R + E +  + RR L  NGL  TPPMGWNSWNHF C++NEN+++ETAD
Sbjct: 21  GSEGGRLLEKKNRTSAEAEHYNVRRYLAENGLGQTPPMGWNSWNHFGCDINENVVRETAD 80

Query: 97  AMVSSALAALGYEYINLDDCWAELNRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIY 156
           AMVS+ LAALGY+YINLDDCWAELNRDS+GN+V  A+ FPSGIKALADYVH KGLKLG+Y
Sbjct: 81  AMVSTGLAALGYQYINLDDCWAELNRDSEGNMVPNAAAFPSGIKALADYVHSKGLKLGVY 140

Query: 157 SDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQS 216
           SDAG QTCSK+MPGSLGHEEQDAKTFASWG+DYLKYDNCEN G S KERYP M KAL  S
Sbjct: 141 SDAGNQTCSKRMPGSLGHEEQDAKTFASWGVDYLKYDNCENLGISVKERYPPMGKALLSS 200

Query: 217 GRPILFSLCEWGQEDPATWAVNVGNSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWN 276
           GRPI FS+CEWG EDP  WA ++GNSWRTT DI+DNWNSMT+IAD NDKWASYA PGGWN
Sbjct: 201 GRPIFFSMCEWGWEDPQIWAKSIGNSWRTTGDIEDNWNSMTSIADSNDKWASYAGPGGWN 260

Query: 277 DPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDK 336
           DPDMLEVGNGGMTT EYRSHFSIWALAKAPLL+GCDIR+MD+ T +L+SN EVIAVNQDK
Sbjct: 261 DPDMLEVGNGGMTTEEYRSHFSIWALAKAPLLVGCDIRAMDDTTHELISNAEVIAVNQDK 320

Query: 337 LGVQGKKVYKFGDLEVWAGPLSGKRVAVVLWNRGFSRVNITASWSAIGLSPSTTVTARDL 396
           LGVQGKKV    DLEVWAGPLS  +VAV+LWNR  SR  +TASWS IGL   TTV ARDL
Sbjct: 321 LGVQGKKVKSTNDLEVWAGPLSDNKVAVILWNRSSSRATVTASWSDIGLQQGTTVDARDL 380

Query: 397 WEHSSQ-MVQNQLTAQVDSHDCKMYVLTP 425
           WEHS+Q +V  +++A++DSH CKMYVLTP
Sbjct: 381 WEHSTQSLVSGEISAEIDSHACKMYVLTP 409

BLAST of Tan0010655 vs. ExPASy Swiss-Prot
Match: Q9FXT4 (Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 6.4e-158
Identity = 259/370 (70.00%), Postives = 292/370 (78.92%), Query Frame = 0

Query: 56  SDSTRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDD 115
           S   RR    NGL  TP MGWNSWNHF C +NE +I+ETADA+V++ LA LGY+Y+N+DD
Sbjct: 48  SRRARRRAFENGLGRTPQMGWNSWNHFYCGINEQIIRETADALVNTGLAKLGYQYVNIDD 107

Query: 116 CWAELNRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHE 175
           CWAE +RDS+GN V    TFPSGIKALADYVH KGLKLGIYSDAG QTCS KMPGSL HE
Sbjct: 108 CWAEYSRDSQGNFVPNRQTFPSGIKALADYVHAKGLKLGIYSDAGSQTCSNKMPGSLDHE 167

Query: 176 EQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATW 235
           EQD KTFASWG+DYLKYDNC + G S  ERY +M+ A++  G+ I FSLCEWG+E+PATW
Sbjct: 168 EQDVKTFASWGVDYLKYDNCNDAGRSVMERYTRMSNAMKTYGKNIFFSLCEWGKENPATW 227

Query: 236 AVNVGNSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRS 295
           A  +GNSWRTT DI DNW SMT+ AD+ND+WA+YA PGGWNDPDMLEVGNGGM+ AEYRS
Sbjct: 228 AGRMGNSWRTTGDIADNWGSMTSRADENDQWAAYAGPGGWNDPDMLEVGNGGMSEAEYRS 287

Query: 296 HFSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAG 355
           HFSIWALAKAPLLIGCD+RSM   T  +LSN EVIAVNQD LGVQGKKV     LEVWAG
Sbjct: 288 HFSIWALAKAPLLIGCDVRSMSQQTKNILSNSEVIAVNQDSLGVQGKKVQSDNGLEVWAG 347

Query: 356 PLSGKRVAVVLWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHSSQMVQNQLTAQVDSH 415
           PLS  R AVVLWNR   +  ITA WS IGL+ S  VTARDLW HSS   Q Q++A V  H
Sbjct: 348 PLSNNRKAVVLWNRQSYQATITAHWSNIGLAGSVAVTARDLWAHSSFAAQGQISASVAPH 407

Query: 416 DCKMYVLTPH 426
           DCKMYVLTP+
Sbjct: 408 DCKMYVLTPN 417

BLAST of Tan0010655 vs. ExPASy Swiss-Prot
Match: Q9FT97 (Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 8.9e-152
Identity = 251/372 (67.47%), Postives = 296/372 (79.57%), Query Frame = 0

Query: 55  DSDSTRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLD 114
           DS+  RR LL NGL +TPPMGWNSWNHF CN++E +IKETADA+V++ L+ LGY Y+N+D
Sbjct: 37  DSEILRRHLLTNGLGVTPPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNID 96

Query: 115 DCWAELNRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGH 174
           DCWAE++RDSKG+LV K STFPSGIKA+ADYVH KGLKLGIYSDAG  TCSK MPGSLG+
Sbjct: 97  DCWAEISRDSKGSLVPKKSTFPSGIKAVADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGY 156

Query: 175 EEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPAT 234
           EE DAKTFA WGIDYLKYDNC + G+ P  RYP MT+AL +SGRPI  SLCEWG   PA 
Sbjct: 157 EEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRYPVMTRALMKSGRPIFHSLCEWGDMHPAL 216

Query: 235 WAVNVGNSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYR 294
           W   VGNSWRTT+DI+D W SM +IAD N+ +A +A+PGGWNDPDMLEVGNGGMT  EY 
Sbjct: 217 WGSPVGNSWRTTNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKDEYI 276

Query: 295 SHFSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWA 354
            HFSIWA++KAPLL+GCDIR+M   T+++++NKEVIA+NQD  GVQ KKV   GDLEVWA
Sbjct: 277 VHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWA 336

Query: 355 GPLSGKRVAVVLWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHSS--QMVQNQLTAQV 414
           GPLSG RVA++L NRG SR +ITA W  I +  ++ V ARDLWEH +  Q     LTA V
Sbjct: 337 GPLSGYRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATV 396

Query: 415 DSHDCKMYVLTP 425
           DSH CK+YVL P
Sbjct: 397 DSHACKLYVLKP 408

BLAST of Tan0010655 vs. NCBI nr
Match: XP_022966963.1 (alpha-galactosidase-like [Cucurbita maxima])

HSP 1 Score: 811.6 bits (2095), Expect = 3.2e-231
Identity = 389/418 (93.06%), Postives = 399/418 (95.45%), Query Frame = 0

Query: 8   LSSPASIMVLFYSVLFWMFMLGSITDGNGGPVAGATRSRPLRFATEFDSDSTRRVLLNNG 67
           +SSPASIM+L YSVLFW F   S  +GNGG +AGATRSRPLRFATEFD DS RRVLLNNG
Sbjct: 2   VSSPASIMLLLYSVLFWTFFFSS--NGNGGAMAGATRSRPLRFATEFDYDSPRRVLLNNG 61

Query: 68  LALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAELNRDSKGN 127
           LALTPPMGWNSWNHFQCN+NENLIKETADAMVSS LAALGY+YINLDDCWAELNRD+KGN
Sbjct: 62  LALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAKGN 121

Query: 128 LVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI 187
           LV K+STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSL HEEQDAKTFASWGI
Sbjct: 122 LVPKSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLDHEEQDAKTFASWGI 181

Query: 188 DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS 247
           DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS
Sbjct: 182 DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS 241

Query: 248 DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL 307
           DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL
Sbjct: 242 DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL 301

Query: 308 LIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAGPLSGKRVAVVLW 367
           LIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYK GDLEVWAG LSGKRVAVVLW
Sbjct: 302 LIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKLGDLEVWAGTLSGKRVAVVLW 361

Query: 368 NRGFSRVNITASWSAIGLSPSTTVTARDLWEHSSQMVQNQLTAQVDSHDCKMYVLTPH 426
           NRGF R  ITASWSAIGLSPSTTVTARDLWEHSSQ+VQ+QLTAQVD HDCKMYVLTPH
Sbjct: 362 NRGFYRAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQLTAQVDPHDCKMYVLTPH 417

BLAST of Tan0010655 vs. NCBI nr
Match: XP_023541567.1 (alpha-galactosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 809.3 bits (2089), Expect = 1.6e-230
Identity = 387/418 (92.58%), Postives = 399/418 (95.45%), Query Frame = 0

Query: 8   LSSPASIMVLFYSVLFWMFMLGSITDGNGGPVAGATRSRPLRFATEFDSDSTRRVLLNNG 67
           +SSPASIM+L YSVLFW F+  S  +GNGG +AGATRSRPLRFA EFD DS RRVLLNNG
Sbjct: 2   VSSPASIMLLLYSVLFWTFLFSS--NGNGGALAGATRSRPLRFAAEFDYDSPRRVLLNNG 61

Query: 68  LALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAELNRDSKGN 127
           LALTPPMGWNSWNHFQCN+NENLIKETADAMVSS LAALGY+YINLDDCWAELNRD+KGN
Sbjct: 62  LALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAKGN 121

Query: 128 LVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI 187
           LV K+STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI
Sbjct: 122 LVPKSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI 181

Query: 188 DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS 247
           DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS
Sbjct: 182 DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS 241

Query: 248 DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL 307
           DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL
Sbjct: 242 DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL 301

Query: 308 LIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAGPLSGKRVAVVLW 367
           LIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVY  GDLEVWAG LSGKRVAVVLW
Sbjct: 302 LIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYILGDLEVWAGALSGKRVAVVLW 361

Query: 368 NRGFSRVNITASWSAIGLSPSTTVTARDLWEHSSQMVQNQLTAQVDSHDCKMYVLTPH 426
           NRGF R  ITASWSAIGLSPSTTVTARDLWEHSSQ+VQ+Q+TAQVD HDCKMYVLTPH
Sbjct: 362 NRGFYRAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQITAQVDPHDCKMYVLTPH 417

BLAST of Tan0010655 vs. NCBI nr
Match: XP_022944991.1 (alpha-galactosidase-like [Cucurbita moschata])

HSP 1 Score: 807.7 bits (2085), Expect = 4.6e-230
Identity = 386/418 (92.34%), Postives = 398/418 (95.22%), Query Frame = 0

Query: 8   LSSPASIMVLFYSVLFWMFMLGSITDGNGGPVAGATRSRPLRFATEFDSDSTRRVLLNNG 67
           +SSPASIM+  YSVLFW F+  S  +GNGG +AGATRSRPLRFA EFD DS RRVLLNNG
Sbjct: 2   VSSPASIMLFLYSVLFWTFLFSS--NGNGGAMAGATRSRPLRFAAEFDYDSPRRVLLNNG 61

Query: 68  LALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAELNRDSKGN 127
           LALTPPMGWNSWNHFQCN+NENLIKETADAMVSS LAALGY+YINLDDCWAELNRD+KGN
Sbjct: 62  LALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAKGN 121

Query: 128 LVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI 187
           LV K+STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI
Sbjct: 122 LVPKSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI 181

Query: 188 DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS 247
           DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS
Sbjct: 182 DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS 241

Query: 248 DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL 307
           DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL
Sbjct: 242 DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL 301

Query: 308 LIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAGPLSGKRVAVVLW 367
           LIGCDIRSMDNITLKLLSNK VIAVNQDKLGVQGKKVYK GDLEVWAG LSGKRVAVVLW
Sbjct: 302 LIGCDIRSMDNITLKLLSNKLVIAVNQDKLGVQGKKVYKLGDLEVWAGALSGKRVAVVLW 361

Query: 368 NRGFSRVNITASWSAIGLSPSTTVTARDLWEHSSQMVQNQLTAQVDSHDCKMYVLTPH 426
           NRGF R  ITASWSAIGLSPSTTVTARDLWEHSSQ+VQ+Q+TAQVD HDCKMYVLTPH
Sbjct: 362 NRGFYRAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQITAQVDPHDCKMYVLTPH 417

BLAST of Tan0010655 vs. NCBI nr
Match: XP_038892679.1 (alpha-galactosidase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 795.8 bits (2054), Expect = 1.8e-226
Identity = 385/425 (90.59%), Postives = 396/425 (93.18%), Query Frame = 0

Query: 1   MAFPSLPLSSPASIMVLFYSVLFWMFMLGSITDGNGGPVAGATRSRPLRFATEFDSDSTR 60
           MA P   LSSP S + L  SVLFWMF+LGS    NGG VAGA RSR LRFA  FDS STR
Sbjct: 1   MASPPFLLSSPPSTIFLLCSVLFWMFLLGS----NGGAVAGAIRSRSLRFAAGFDSVSTR 60

Query: 61  RVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAEL 120
           RVLLNNGLALTPPMGWNSWNHFQCN+NENLIKETADAMVSS LA+LGYEYINLDDCWAEL
Sbjct: 61  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLASLGYEYINLDDCWAEL 120

Query: 121 NRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAK 180
           NRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHE+QDA+
Sbjct: 121 NRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEQQDAE 180

Query: 181 TFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVG 240
           TFASWGIDYLKYDNCENTGTSPKERYPKM+KALQQSGRPILFSLCEWGQEDPATWA+NVG
Sbjct: 181 TFASWGIDYLKYDNCENTGTSPKERYPKMSKALQQSGRPILFSLCEWGQEDPATWAINVG 240

Query: 241 NSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIW 300
           NSWRTTSDIQDNW SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTT EYRSHFSIW
Sbjct: 241 NSWRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIW 300

Query: 301 ALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAGPLSGK 360
           ALAKAPLLIGCDIRSMDNI LKLLSNKEVIAVNQDKLGVQGKKVYK+GDLEVWAGPLSGK
Sbjct: 301 ALAKAPLLIGCDIRSMDNIALKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGK 360

Query: 361 RVAVVLWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHSSQMVQNQLTAQVDSHDCKMY 420
           RVAVVLWNRG  R NITASWS IGLS STTVTARDLWEHSSQ+VQ+ LTAQVDSHDCKMY
Sbjct: 361 RVAVVLWNRGLWRANITASWSDIGLSTSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMY 420

Query: 421 VLTPH 426
           VLTPH
Sbjct: 421 VLTPH 421

BLAST of Tan0010655 vs. NCBI nr
Match: KAG6573784.1 (Alpha-galactosidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 791.2 bits (2042), Expect = 4.5e-225
Identity = 379/422 (89.81%), Postives = 397/422 (94.08%), Query Frame = 0

Query: 4   PSLPLSSPASIMVLFYSVLFWMFMLGSITDGNGGPVAGATRSRPLRFATEFDSDSTRRVL 63
           PS  +SSPASIM+  YSVLFW F+  S  +GNGG +AGATRSRPLRFA EFD DS RRVL
Sbjct: 271 PSAMVSSPASIMLFLYSVLFWTFLFSS--NGNGGAMAGATRSRPLRFAAEFDYDSPRRVL 330

Query: 64  LNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAELNRD 123
           LNNGLALTPPMGWNSWNHFQCN+NENLIKETADAMVSS LAALGY+YINLDDCWAELNRD
Sbjct: 331 LNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRD 390

Query: 124 SKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFA 183
           +KGNLV K+STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFA
Sbjct: 391 AKGNLVPKSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFA 450

Query: 184 SWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSW 243
           SWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSW
Sbjct: 451 SWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSW 510

Query: 244 RTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALA 303
           RTTSDIQD+WNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALA
Sbjct: 511 RTTSDIQDDWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALA 570

Query: 304 KAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAGPLSGKRVA 363
           KAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKV+K GDLEVWAG LSGKRVA
Sbjct: 571 KAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVFKLGDLEVWAGALSGKRVA 630

Query: 364 VVLWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHSSQMVQNQLTAQVDSHDCKMYVLT 423
           VVLWNRGF R  ITASWSAIGLSPST+VTARDLWEHSSQ+VQ+Q+TAQVD HD  +  +T
Sbjct: 631 VVLWNRGFYRAKITASWSAIGLSPSTSVTARDLWEHSSQVVQHQITAQVDPHDIFLKSVT 690

Query: 424 PH 426
           P+
Sbjct: 691 PN 690

BLAST of Tan0010655 vs. ExPASy TrEMBL
Match: A0A6J1HTR9 (Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466515 PE=3 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 1.5e-231
Identity = 389/418 (93.06%), Postives = 399/418 (95.45%), Query Frame = 0

Query: 8   LSSPASIMVLFYSVLFWMFMLGSITDGNGGPVAGATRSRPLRFATEFDSDSTRRVLLNNG 67
           +SSPASIM+L YSVLFW F   S  +GNGG +AGATRSRPLRFATEFD DS RRVLLNNG
Sbjct: 2   VSSPASIMLLLYSVLFWTFFFSS--NGNGGAMAGATRSRPLRFATEFDYDSPRRVLLNNG 61

Query: 68  LALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAELNRDSKGN 127
           LALTPPMGWNSWNHFQCN+NENLIKETADAMVSS LAALGY+YINLDDCWAELNRD+KGN
Sbjct: 62  LALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAKGN 121

Query: 128 LVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI 187
           LV K+STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSL HEEQDAKTFASWGI
Sbjct: 122 LVPKSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLDHEEQDAKTFASWGI 181

Query: 188 DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS 247
           DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS
Sbjct: 182 DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS 241

Query: 248 DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL 307
           DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL
Sbjct: 242 DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL 301

Query: 308 LIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAGPLSGKRVAVVLW 367
           LIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYK GDLEVWAG LSGKRVAVVLW
Sbjct: 302 LIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKLGDLEVWAGTLSGKRVAVVLW 361

Query: 368 NRGFSRVNITASWSAIGLSPSTTVTARDLWEHSSQMVQNQLTAQVDSHDCKMYVLTPH 426
           NRGF R  ITASWSAIGLSPSTTVTARDLWEHSSQ+VQ+QLTAQVD HDCKMYVLTPH
Sbjct: 362 NRGFYRAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQLTAQVDPHDCKMYVLTPH 417

BLAST of Tan0010655 vs. ExPASy TrEMBL
Match: A0A6J1FZL3 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111449362 PE=3 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 2.2e-230
Identity = 386/418 (92.34%), Postives = 398/418 (95.22%), Query Frame = 0

Query: 8   LSSPASIMVLFYSVLFWMFMLGSITDGNGGPVAGATRSRPLRFATEFDSDSTRRVLLNNG 67
           +SSPASIM+  YSVLFW F+  S  +GNGG +AGATRSRPLRFA EFD DS RRVLLNNG
Sbjct: 2   VSSPASIMLFLYSVLFWTFLFSS--NGNGGAMAGATRSRPLRFAAEFDYDSPRRVLLNNG 61

Query: 68  LALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAELNRDSKGN 127
           LALTPPMGWNSWNHFQCN+NENLIKETADAMVSS LAALGY+YINLDDCWAELNRD+KGN
Sbjct: 62  LALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAKGN 121

Query: 128 LVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI 187
           LV K+STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI
Sbjct: 122 LVPKSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGI 181

Query: 188 DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS 247
           DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS
Sbjct: 182 DYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTS 241

Query: 248 DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL 307
           DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL
Sbjct: 242 DIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPL 301

Query: 308 LIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAGPLSGKRVAVVLW 367
           LIGCDIRSMDNITLKLLSNK VIAVNQDKLGVQGKKVYK GDLEVWAG LSGKRVAVVLW
Sbjct: 302 LIGCDIRSMDNITLKLLSNKLVIAVNQDKLGVQGKKVYKLGDLEVWAGALSGKRVAVVLW 361

Query: 368 NRGFSRVNITASWSAIGLSPSTTVTARDLWEHSSQMVQNQLTAQVDSHDCKMYVLTPH 426
           NRGF R  ITASWSAIGLSPSTTVTARDLWEHSSQ+VQ+Q+TAQVD HDCKMYVLTPH
Sbjct: 362 NRGFYRAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQITAQVDPHDCKMYVLTPH 417

BLAST of Tan0010655 vs. ExPASy TrEMBL
Match: A0A1S3BEH7 (Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488794 PE=3 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 6.9e-224
Identity = 382/421 (90.74%), Postives = 396/421 (94.06%), Query Frame = 0

Query: 5   SLPLSSPASIMVLFYSVLFWMFMLGSITDGNGGPVAGATRSRPLRFATEFDSDSTRRVLL 64
           +L  SSP S+MVL Y VL W F+LG   +GNG  VAGA+RS  LRFA EFDS S+RRVLL
Sbjct: 2   ALSPSSP-SVMVLLYFVLSWTFLLGG--NGNGVAVAGASRSTALRFAAEFDSVSSRRVLL 61

Query: 65  NNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAELNRDS 124
           NNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSS LAALGYEYINLDDCWAEL+RDS
Sbjct: 62  NNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCWAELDRDS 121

Query: 125 KGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFAS 184
           KGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLG+EEQDAKTFAS
Sbjct: 122 KGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGNEEQDAKTFAS 181

Query: 185 WGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWR 244
           WGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWR
Sbjct: 182 WGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWR 241

Query: 245 TTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAK 304
           TTSDIQDNW SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAK
Sbjct: 242 TTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAK 301

Query: 305 APLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAGPLSGKRVAV 364
           APLLIGCDIRSMDNIT+KLLSNKEVIAVNQDKLGVQGKKVYK+GDLEVWAGPLSGKRVAV
Sbjct: 302 APLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAV 361

Query: 365 VLWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHSSQMVQNQLTAQVDSHDCKMYVLTP 424
           VLWNRG  R NITASWS IGL  STTVTARDLW+HSSQ+VQ+ LTAQVDSHDCKM+VLTP
Sbjct: 362 VLWNRGLWRANITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFVLTP 419

Query: 425 H 426
           H
Sbjct: 422 H 419

BLAST of Tan0010655 vs. ExPASy TrEMBL
Match: A0A5A7SVZ2 (Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003280 PE=3 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 1.7e-222
Identity = 382/422 (90.52%), Postives = 396/422 (93.84%), Query Frame = 0

Query: 5   SLPLSSPASIMVLFYSVLFWMFMLGSITDGNGGPVAGATRSRPLRFATEFDSDSTRRVLL 64
           +L  SSP S+MVL Y VL W F+LG   +GNG  VAGA+RS  LRFA EFDS S+RRVLL
Sbjct: 2   ALSPSSP-SVMVLLYFVLSWTFLLGG--NGNGVAVAGASRSTALRFAAEFDSVSSRRVLL 61

Query: 65  NNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAELNRDS 124
           NNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSS LAALGYEYINLDDCWAEL+RDS
Sbjct: 62  NNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCWAELDRDS 121

Query: 125 KGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFAS 184
           KGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLG+EEQDAKTFAS
Sbjct: 122 KGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGNEEQDAKTFAS 181

Query: 185 WGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWR 244
           WGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWR
Sbjct: 182 WGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWR 241

Query: 245 TTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAK 304
           TTSDIQDNW SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAK
Sbjct: 242 TTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAK 301

Query: 305 APLLIGCDIRSMDNITLKLLSNKEVIAVNQ-DKLGVQGKKVYKFGDLEVWAGPLSGKRVA 364
           APLLIGCDIRSMDNIT+KLLSNKEVIAVNQ DKLGVQGKKVYK+GDLEVWAGPLSGKRVA
Sbjct: 302 APLLIGCDIRSMDNITMKLLSNKEVIAVNQVDKLGVQGKKVYKYGDLEVWAGPLSGKRVA 361

Query: 365 VVLWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHSSQMVQNQLTAQVDSHDCKMYVLT 424
           VVLWNRG  R NITASWS IGL  STTVTARDLW+HSSQ+VQ+ LTAQVDSHDCKM+VLT
Sbjct: 362 VVLWNRGLWRANITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFVLT 420

Query: 425 PH 426
           PH
Sbjct: 422 PH 420

BLAST of Tan0010655 vs. ExPASy TrEMBL
Match: Q2HYY3 (Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_5G580630 PE=2 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 7.2e-221
Identity = 373/421 (88.60%), Postives = 394/421 (93.59%), Query Frame = 0

Query: 5   SLPLSSPASIMVLFYSVLFWMFMLGSITDGNGGPVAGATRSRPLRFATEFDSDSTRRVLL 64
           +LP SSP S+M+L Y +LFW F+LG   +GNG  VA A+RS  LRFATEFDS S+RR+LL
Sbjct: 2   ALPPSSP-SVMLLLYFLLFWTFLLGGNGNGNGVVVAAASRSTALRFATEFDSASSRRILL 61

Query: 65  NNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAELNRDS 124
           NNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVS+ LAALGY+YINLDDCWAEL+RDS
Sbjct: 62  NNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSTGLAALGYQYINLDDCWAELDRDS 121

Query: 125 KGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFAS 184
           KGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGI+TCSK+MPGSLGHEEQDAKTFAS
Sbjct: 122 KGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIRTCSKRMPGSLGHEEQDAKTFAS 181

Query: 185 WGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWR 244
           WGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWR
Sbjct: 182 WGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWR 241

Query: 245 TTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAK 304
           TTSDIQDNW SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMT AEYRSHFSIWALAK
Sbjct: 242 TTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTIAEYRSHFSIWALAK 301

Query: 305 APLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAGPLSGKRVAV 364
           APLLIGCDIRSMDN T+KLLSNKEVIAVNQDKLGVQGKKV+K+GDLEVWAG LSGKRVAV
Sbjct: 302 APLLIGCDIRSMDNNTMKLLSNKEVIAVNQDKLGVQGKKVHKYGDLEVWAGLLSGKRVAV 361

Query: 365 VLWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHSSQMVQNQLTAQVDSHDCKMYVLTP 424
           VLWNR   R NITA WS IGLS STTVTARDLWEHSSQ+V++ LTAQVDSHDCKM+VLTP
Sbjct: 362 VLWNRSLWRANITAYWSDIGLSSSTTVTARDLWEHSSQVVRHHLTAQVDSHDCKMFVLTP 421

Query: 425 H 426
           H
Sbjct: 422 H 421

BLAST of Tan0010655 vs. TAIR 10
Match: AT5G08370.1 (alpha-galactosidase 2 )

HSP 1 Score: 624.4 bits (1609), Expect = 6.7e-179
Identity = 290/364 (79.67%), Postives = 321/364 (88.19%), Query Frame = 0

Query: 61  RVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAEL 120
           R+L+NNGLAL+P MGWNSWNHFQCN+NE LIK+TADAMVSS L+A+GY+YIN+DDCW EL
Sbjct: 29  RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGEL 88

Query: 121 NRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAK 180
            RDS+G+LVAKASTFPSGIKAL+DYVH KGLKLGIYSDAG  TCS+ MPGSLGHEEQDAK
Sbjct: 89  KRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAK 148

Query: 181 TFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVG 240
           TFASWGIDYLKYDNCENTGTSP+ERYPKM+KAL  SGR I FSLCEWGQEDPATWA ++G
Sbjct: 149 TFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG 208

Query: 241 NSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIW 300
           NSWRTT DIQDNW SMT IADQND+WASYA+PG WNDPDMLEVGNGGMT  EY SHFSIW
Sbjct: 209 NSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIW 268

Query: 301 ALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAGPLSGK 360
           ALAKAPLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV K GDLEVWAGPLS K
Sbjct: 269 ALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKK 328

Query: 361 RVAVVLWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHSS-QMVQNQLTAQVDSHDCKM 420
           RVAV+LWNRG +  NITA W+ IGL+ S  V ARDLWEHS+   V+ QL+A V+ H CKM
Sbjct: 329 RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSALVEPHACKM 388

Query: 421 YVLT 424
           Y LT
Sbjct: 389 YTLT 392

BLAST of Tan0010655 vs. TAIR 10
Match: AT5G08370.2 (alpha-galactosidase 2 )

HSP 1 Score: 624.4 bits (1609), Expect = 6.7e-179
Identity = 290/364 (79.67%), Postives = 321/364 (88.19%), Query Frame = 0

Query: 61  RVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAEL 120
           R+L+NNGLAL+P MGWNSWNHFQCN+NE LIK+TADAMVSS L+A+GY+YIN+DDCW EL
Sbjct: 3   RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGEL 62

Query: 121 NRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAK 180
            RDS+G+LVAKASTFPSGIKAL+DYVH KGLKLGIYSDAG  TCS+ MPGSLGHEEQDAK
Sbjct: 63  KRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAK 122

Query: 181 TFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVG 240
           TFASWGIDYLKYDNCENTGTSP+ERYPKM+KAL  SGR I FSLCEWGQEDPATWA ++G
Sbjct: 123 TFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG 182

Query: 241 NSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIW 300
           NSWRTT DIQDNW SMT IADQND+WASYA+PG WNDPDMLEVGNGGMT  EY SHFSIW
Sbjct: 183 NSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIW 242

Query: 301 ALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWAGPLSGK 360
           ALAKAPLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV K GDLEVWAGPLS K
Sbjct: 243 ALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKK 302

Query: 361 RVAVVLWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHSS-QMVQNQLTAQVDSHDCKM 420
           RVAV+LWNRG +  NITA W+ IGL+ S  V ARDLWEHS+   V+ QL+A V+ H CKM
Sbjct: 303 RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSALVEPHACKM 362

Query: 421 YVLT 424
           Y LT
Sbjct: 363 YTLT 366

BLAST of Tan0010655 vs. TAIR 10
Match: AT5G08380.1 (alpha-galactosidase 1 )

HSP 1 Score: 538.1 bits (1385), Expect = 6.3e-153
Identity = 251/372 (67.47%), Postives = 296/372 (79.57%), Query Frame = 0

Query: 55  DSDSTRRVLLNNGLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLD 114
           DS+  RR LL NGL +TPPMGWNSWNHF CN++E +IKETADA+V++ L+ LGY Y+N+D
Sbjct: 37  DSEILRRHLLTNGLGVTPPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNID 96

Query: 115 DCWAELNRDSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGH 174
           DCWAE++RDSKG+LV K STFPSGIKA+ADYVH KGLKLGIYSDAG  TCSK MPGSLG+
Sbjct: 97  DCWAEISRDSKGSLVPKKSTFPSGIKAVADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGY 156

Query: 175 EEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPAT 234
           EE DAKTFA WGIDYLKYDNC + G+ P  RYP MT+AL +SGRPI  SLCEWG   PA 
Sbjct: 157 EEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRYPVMTRALMKSGRPIFHSLCEWGDMHPAL 216

Query: 235 WAVNVGNSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYR 294
           W   VGNSWRTT+DI+D W SM +IAD N+ +A +A+PGGWNDPDMLEVGNGGMT  EY 
Sbjct: 217 WGSPVGNSWRTTNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKDEYI 276

Query: 295 SHFSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGDLEVWA 354
            HFSIWA++KAPLL+GCDIR+M   T+++++NKEVIA+NQD  GVQ KKV   GDLEVWA
Sbjct: 277 VHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWA 336

Query: 355 GPLSGKRVAVVLWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHSS--QMVQNQLTAQV 414
           GPLSG RVA++L NRG SR +ITA W  I +  ++ V ARDLWEH +  Q     LTA V
Sbjct: 337 GPLSGYRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATV 396

Query: 415 DSHDCKMYVLTP 425
           DSH CK+YVL P
Sbjct: 397 DSHACKLYVLKP 408

BLAST of Tan0010655 vs. TAIR 10
Match: AT3G56310.1 (Melibiase family protein )

HSP 1 Score: 516.9 bits (1330), Expect = 1.5e-146
Identity = 254/421 (60.33%), Postives = 300/421 (71.26%), Query Frame = 0

Query: 20  SVLFWMFMLGSITDGNGGPVAGATRSRPLR-----------FATEFDSDSTRRVLLNNGL 79
           SVLF +  L S++      +AG  ++  L+           F + +D+    R+ LNNGL
Sbjct: 10  SVLFLVVGLFSLSVLVSQSIAGRVKAPLLQSNTGGLVFSKSFNSIYDTSMYGRLQLNNGL 69

Query: 80  ALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAELNRDSKGNL 139
           A TP MGWNSWN F CN+NE +IKETADA+VSS LA LGY ++N+DDCW+ L RDS+G L
Sbjct: 70  ARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNIDDCWSNLLRDSEGQL 129

Query: 140 VAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGID 199
           V    TFPSGIK LADYVH KGLKLGIYSDAG+ TC +  PGSL HE  DA  FASWG+D
Sbjct: 130 VPHPETFPSGIKLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFHEVDDADIFASWGVD 189

Query: 200 YLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSD 259
           YLKYDNC N G  P ERYP M  AL  +GR I +SLCEWG +DPA WA  VGNSWRTT D
Sbjct: 190 YLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEVGNSWRTTDD 249

Query: 260 IQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLL 319
           I D W SMTTIAD N+KWA+YA PGGWNDPDMLE+GNGGMT  EYR HFSIWAL KAPLL
Sbjct: 250 INDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYRGHFSIWALMKAPLL 309

Query: 320 IGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGD---LEVWAGPLSGKRVAVV 379
           IGCD+R+M   TL++LSNKE+IAVNQD LGVQG+K+   G+    +VW+GPLSG R+ V 
Sbjct: 310 IGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVA 369

Query: 380 LWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHS--SQMVQNQLTAQVDSHDCKMYVLT 425
           LWNR      ITASW  IGL  + +V+ RDLW+H   ++       AQVD+HDC MYVLT
Sbjct: 370 LWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLT 429

BLAST of Tan0010655 vs. TAIR 10
Match: AT3G56310.2 (Melibiase family protein )

HSP 1 Score: 470.3 bits (1209), Expect = 1.6e-132
Identity = 239/421 (56.77%), Postives = 283/421 (67.22%), Query Frame = 0

Query: 20  SVLFWMFMLGSITDGNGGPVAGATRSRPLR-----------FATEFDSDSTRRVLLNNGL 79
           SVLF +  L S++      +AG  ++  L+           F + +D+    R+ LNNGL
Sbjct: 10  SVLFLVVGLFSLSVLVSQSIAGRVKAPLLQSNTGGLVFSKSFNSIYDTSMYGRLQLNNGL 69

Query: 80  ALTPPMGWNSWNHFQCNLNENLIKETADAMVSSALAALGYEYINLDDCWAELNRDSKGNL 139
           A TP MGWNSWN F CN+NE +IKETADA+VSS LA LGY ++N+               
Sbjct: 70  ARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNI--------------- 129

Query: 140 VAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGID 199
                    GIK LADYVH KGLKLGIYSDAG+ TC +  PGSL HE  DA  FASWG+D
Sbjct: 130 ---------GIKLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFHEVDDADIFASWGVD 189

Query: 200 YLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSD 259
           YLKYDNC N G  P ERYP M  AL  +GR I +SLCEWG +DPA WA  VGNSWRTT D
Sbjct: 190 YLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEVGNSWRTTDD 249

Query: 260 IQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLL 319
           I D W SMTTIAD N+KWA+YA PGGWNDPDMLE+GNGGMT  EYR HFSIWAL KAPLL
Sbjct: 250 INDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYRGHFSIWALMKAPLL 309

Query: 320 IGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKFGD---LEVWAGPLSGKRVAVV 379
           IGCD+R+M   TL++LSNKE+IAVNQD LGVQG+K+   G+    +VW+GPLSG R+ V 
Sbjct: 310 IGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVA 369

Query: 380 LWNRGFSRVNITASWSAIGLSPSTTVTARDLWEHS--SQMVQNQLTAQVDSHDCKMYVLT 425
           LWNR      ITASW  IGL  + +V+ RDLW+H   ++       AQVD+HDC MYVLT
Sbjct: 370 LWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLT 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RX869.5e-17879.67Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1[more]
Q426563.6e-17779.67Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1[more]
P147491.0e-17673.78Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1[more]
Q9FXT46.4e-15870.00Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=... [more]
Q9FT978.9e-15267.47Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022966963.13.2e-23193.06alpha-galactosidase-like [Cucurbita maxima][more]
XP_023541567.11.6e-23092.58alpha-galactosidase-like [Cucurbita pepo subsp. pepo][more]
XP_022944991.14.6e-23092.34alpha-galactosidase-like [Cucurbita moschata][more]
XP_038892679.11.8e-22690.59alpha-galactosidase-like isoform X2 [Benincasa hispida][more]
KAG6573784.14.5e-22589.81Alpha-galactosidase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1HTR91.5e-23193.06Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466515 PE=3 SV=1[more]
A0A6J1FZL32.2e-23092.34Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111449362 PE=3 SV=1[more]
A0A1S3BEH76.9e-22490.74Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488794 PE=3 SV=1[more]
A0A5A7SVZ21.7e-22290.52Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G0... [more]
Q2HYY37.2e-22188.60Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_5G580630 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT5G08370.16.7e-17979.67alpha-galactosidase 2 [more]
AT5G08370.26.7e-17979.67alpha-galactosidase 2 [more]
AT5G08380.16.3e-15367.47alpha-galactosidase 1 [more]
AT3G56310.11.5e-14660.33Melibiase family protein [more]
AT3G56310.21.6e-13256.77Melibiase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002241Glycoside hydrolase, family 27PRINTSPR00740GLHYDRLASE27coord: 159..180
score: 62.5
coord: 307..328
score: 55.91
coord: 82..101
score: 66.5
coord: 118..133
score: 62.81
coord: 221..239
score: 52.37
coord: 286..305
score: 69.5
coord: 194..211
score: 76.39
IPR002241Glycoside hydrolase, family 27PFAMPF16499Melibiase_2coord: 87..351
e-value: 9.9E-81
score: 271.0
IPR002241Glycoside hydrolase, family 27PANTHERPTHR11452ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASEcoord: 71..416
IPR002241Glycoside hydrolase, family 27CDDcd14792GH27coord: 88..351
e-value: 6.1621E-161
score: 452.01
IPR041233Alpha galactosidase, C-terminal beta sandwich domainPFAMPF17801Melibiase_Ccoord: 364..414
e-value: 4.2E-13
score: 49.2
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 80..353
e-value: 2.9E-139
score: 465.3
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 354..418
e-value: 6.7E-20
score: 72.8
NoneNo IPR availablePANTHERPTHR11452:SF33ALPHA-GALACTOSIDASE 2coord: 71..416
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 346..414
IPR000111Glycoside hydrolase family 27/36, conserved sitePROSITEPS00512ALPHA_GALACTOSIDASEcoord: 123..139
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 78..351

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0010655.1Tan0010655.1mRNA
Tan0010655.2Tan0010655.2mRNA
Tan0010655.3Tan0010655.3mRNA
Tan0010655.4Tan0010655.4mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0009965 leaf morphogenesis
biological_process GO:0009911 positive regulation of flower development
biological_process GO:0009620 response to fungus
cellular_component GO:0048046 apoplast
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009505 plant-type cell wall
molecular_function GO:0052692 raffinose alpha-galactosidase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds