Tan0010485 (gene) Snake gourd v1

Overview


Name	Tan0010485
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	mitogen-activated protein kinase kinase kinase 17-like
Location	LG09: 66370303 .. 66371352 (+)
RNA-Seq Expression	Tan0010485
Synteny	Tan0010485

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGAAATCCTGTGTTGCTTCAGTTGTGAAAGAGCAGCAAAGTCATTACTTTGTGAACTTACACGATTTCTTGAAAACGAAGAGAGGCATTGTGAAGTGTGCAAAGAAGACGTTGTGGAACAACAACAATTGGAATGACAATGTCAAACATAATAATGGAAGATTTGCTAATTTTAATGTCGAAGAATTTCATGGTTCTTTTTGGATTGGAGATTGTGTTTTTCGCAAACGTGGGTCTGGTCTTATTTTTTTGGCAAAGAACAAAACACCCAACAACCCAAACTCTTCACAACTAATTCCCACACAGATTGTTGTCAAATCTTGCTTCATGGAATTCTCTTCTTCACTTCAACATGAGAAGGAAGTGCTATTCAATTTGGCACCCTGCACCAATGTGGTGCGTTGTTATGGCGATGAAATAACCACCACCAAATCTGGTGAAAAATTTTACAATCTCCTTTTCGAGTATTGTTCTGGCTCTAGCTTGCGTGATTACATTATCAATTTTGGACTTAATGGATTGTCCGAATTTGAAGTTCGTCGGTATACGAGGGACATTGTCCATGGACTCTACTACATGCATTGTAATGCTAATGCTCGCTACATTCACGGTGATATAAAACCAAGAAACATCTTATTATCCCCTAGTGGCAAAGGTTGTTTCGTGGCTAAGTTAGCTAGTTTTGGATTGGCTAAGAACCTAACTCCCGAAGCATTATGCGATCAAGAAATCAGTGGTTCAGGATCCTACATATCTCCAGAGTTGGCAAGAGATGGTTATTTAGATTGGCCTGCTGACATTTGGGCACTTGGATGCGTGGTGTTCGAGATGTTGACCGGAAAACCAGCATGGAGCTTTGAAGAAGCTCATAATTACATGTTGGGTTGCTACCACAGGGACCTTCCAGAAATTCCATATGAGATATCAAGTGCCGGAAGGGATTTTATTAAAAAGTGTTTGATAAGGAGTCCATATAAAAGAAGACCAATTTGGTTGTTGATCAAACATCCTTTTGTTTGCAATCTGAACAACTCATATGTTGAATGA

mRNA sequence

ATGACGAAATCCTGTGTTGCTTCAGTTGTGAAAGAGCAGCAAAGTCATTACTTTGTGAACTTACACGATTTCTTGAAAACGAAGAGAGGCATTGTGAAGTGTGCAAAGAAGACGTTGTGGAACAACAACAATTGGAATGACAATGTCAAACATAATAATGGAAGATTTGCTAATTTTAATGTCGAAGAATTTCATGGTTCTTTTTGGATTGGAGATTGTGTTTTTCGCAAACGTGGGTCTGGTCTTATTTTTTTGGCAAAGAACAAAACACCCAACAACCCAAACTCTTCACAACTAATTCCCACACAGATTGTTGTCAAATCTTGCTTCATGGAATTCTCTTCTTCACTTCAACATGAGAAGGAAGTGCTATTCAATTTGGCACCCTGCACCAATGTGGTGCGTTGTTATGGCGATGAAATAACCACCACCAAATCTGGTGAAAAATTTTACAATCTCCTTTTCGAGTATTGTTCTGGCTCTAGCTTGCGTGATTACATTATCAATTTTGGACTTAATGGATTGTCCGAATTTGAAGTTCGTCGGTATACGAGGGACATTGTCCATGGACTCTACTACATGCATTGTAATGCTAATGCTCGCTACATTCACGGTGATATAAAACCAAGAAACATCTTATTATCCCCTAGTGGCAAAGGTTGTTTCGTGGCTAAGTTAGCTAGTTTTGGATTGGCTAAGAACCTAACTCCCGAAGCATTATGCGATCAAGAAATCAGTGGTTCAGGATCCTACATATCTCCAGAGTTGGCAAGAGATGGTTATTTAGATTGGCCTGCTGACATTTGGGCACTTGGATGCGTGGTGTTCGAGATGTTGACCGGAAAACCAGCATGGAGCTTTGAAGAAGCTCATAATTACATGTTGGGTTGCTACCACAGGGACCTTCCAGAAATTCCATATGAGATATCAAGTGCCGGAAGGGATTTTATTAAAAAGTGTTTGATAAGGAGTCCATATAAAAGAAGACCAATTTGGTTGTTGATCAAACATCCTTTTGTTTGCAATCTGAACAACTCATATGTTGAATGA

Coding sequence (CDS)

ATGACGAAATCCTGTGTTGCTTCAGTTGTGAAAGAGCAGCAAAGTCATTACTTTGTGAACTTACACGATTTCTTGAAAACGAAGAGAGGCATTGTGAAGTGTGCAAAGAAGACGTTGTGGAACAACAACAATTGGAATGACAATGTCAAACATAATAATGGAAGATTTGCTAATTTTAATGTCGAAGAATTTCATGGTTCTTTTTGGATTGGAGATTGTGTTTTTCGCAAACGTGGGTCTGGTCTTATTTTTTTGGCAAAGAACAAAACACCCAACAACCCAAACTCTTCACAACTAATTCCCACACAGATTGTTGTCAAATCTTGCTTCATGGAATTCTCTTCTTCACTTCAACATGAGAAGGAAGTGCTATTCAATTTGGCACCCTGCACCAATGTGGTGCGTTGTTATGGCGATGAAATAACCACCACCAAATCTGGTGAAAAATTTTACAATCTCCTTTTCGAGTATTGTTCTGGCTCTAGCTTGCGTGATTACATTATCAATTTTGGACTTAATGGATTGTCCGAATTTGAAGTTCGTCGGTATACGAGGGACATTGTCCATGGACTCTACTACATGCATTGTAATGCTAATGCTCGCTACATTCACGGTGATATAAAACCAAGAAACATCTTATTATCCCCTAGTGGCAAAGGTTGTTTCGTGGCTAAGTTAGCTAGTTTTGGATTGGCTAAGAACCTAACTCCCGAAGCATTATGCGATCAAGAAATCAGTGGTTCAGGATCCTACATATCTCCAGAGTTGGCAAGAGATGGTTATTTAGATTGGCCTGCTGACATTTGGGCACTTGGATGCGTGGTGTTCGAGATGTTGACCGGAAAACCAGCATGGAGCTTTGAAGAAGCTCATAATTACATGTTGGGTTGCTACCACAGGGACCTTCCAGAAATTCCATATGAGATATCAAGTGCCGGAAGGGATTTTATTAAAAAGTGTTTGATAAGGAGTCCATATAAAAGAAGACCAATTTGGTTGTTGATCAAACATCCTTTTGTTTGCAATCTGAACAACTCATATGTTGAATGA

Protein sequence

MTKSCVASVVKEQQSHYFVNLHDFLKTKRGIVKCAKKTLWNNNNWNDNVKHNNGRFANFNVEEFHGSFWIGDCVFRKRGSGLIFLAKNKTPNNPNSSQLIPTQIVVKSCFMEFSSSLQHEKEVLFNLAPCTNVVRCYGDEITTTKSGEKFYNLLFEYCSGSSLRDYIINFGLNGLSEFEVRRYTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGKGCFVAKLASFGLAKNLTPEALCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEMLTGKPAWSFEEAHNYMLGCYHRDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWLLIKHPFVCNLNNSYVE

Homology

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BLAST of Tan0010485 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.2e-42
Identity = 99/259 (38.22%), Postives = 141/259 (54.44%), Query Frame = 0


Query: 90  TPNNPNSSQLIPTQIVVKSCFMEFSSSLQHEKEVLFNLAPCTNVVRCYGDEITTTKSGEK 149
           T     +S   P  I VKS     ++SL +EK VL +L  C  ++RCYG++ +T ++GE+
Sbjct: 19  TATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIRCYGED-STVENGEE 78

Query: 150 FYNLLFEYCSGSSLRDYIINFGLNGLSEFEVRRYTRDIVHGLYYMHCNANARYIHGDIKP 209
            +NLL EY S  SL  Y+   G  GL E  VRR+T  ++ GL ++H      + H DIK 
Sbjct: 79  MHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIHAKG---FAHCDIKL 138

Query: 210 RNILLSPSGKGCFVAKLASFGLAKNLTPEALC---DQEISGSGSYISPELARDGYLDWPA 269
            NILL   G      K+A FGLA  +  +        EI G+  Y++PE   D      A
Sbjct: 139 ANILLFNDGS----VKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPECVNDNEYGSAA 198

Query: 270 DIWALGCVVFEMLTGKPAWSFEEAHNYM-----LGCYHRDLPEIPYEISSAGRDFIKKCL 329
           D+WALGC V EM +GK AWS +E  ++M     +G    +LP+IP  +S  G+DF+ KC 
Sbjct: 199 DVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIG-VGDELPKIPEMLSEEGKDFLSKCF 258

Query: 330 IRSPYKRRPIWLLIKHPFV 341
           ++ P KR    +L+ H FV
Sbjct: 259 VKDPAKRWTAEMLLNHSFV 268

[Close]

BLAST of Tan0010485 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.2e-31
Identity = 81/243 (33.33%), Postives = 129/243 (53.09%), Query Frame = 0


Query: 104 IVVKSCFMEFSSSLQHEKEVLFNLAPCTNVVRCYGDEITTTKSGEKFYNLLFEYCSGSSL 163
           + VKS  +  S  LQ E ++L +L+    V+   G E     +G   YNLL EY    +L
Sbjct: 29  LAVKSSEVHRSEFLQREAKILSSLS-SPYVIGYRGSETKRESNGVVMYNLLMEYAPYGTL 88

Query: 164 RDYIINFGLNGLSEFEVRRYTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGKGCFV 223
            D     G   + E  V +YTRDI+ GL Y+H       +H D+K  N+++S  G+    
Sbjct: 89  TDAAAKDG-GRVDETRVVKYTRDILKGLEYIHSKG---IVHCDVKGSNVVISEKGE---- 148

Query: 224 AKLASFGLAKNLTPEALCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEMLTGKP 283
           AK+A FG AK + P  + +  + G+ ++++PE+AR       +DIWA+GC + EM+TG P
Sbjct: 149 AKIADFGCAKRVDP--VFESPVMGTPAFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSP 208

Query: 284 AWSFEEAHN------YMLGCYHRDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWLLIKH 341
            W+  ++        Y +G Y  + PE+P  ++   +DF++KCL R   +R     L+ H
Sbjct: 209 PWTKADSREDPVSVLYRVG-YSSETPELPCLLAEEAKDFLEKCLKREANERWTATQLLNH 259

[Close]

BLAST of Tan0010485 vs. ExPASy Swiss-Prot
Match: Q9C5H5 (Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK5 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.2e-29
Identity = 85/238 (35.71%), Postives = 126/238 (52.94%), Query Frame = 0


Query: 112 EFSSSLQHEKEVLFNLAPCTNVVRCYGDEITTTKSGEKFYNLLFEYCSGSSLRDYIINFG 171
           E    L+ E ++L NL    N+V+ +G E       E  + +  EY    S+  YI +  
Sbjct: 388 ECIKQLEQEIKLLSNLQH-PNIVQYFGSETV-----EDRFFIYLEYVHPGSINKYIRDH- 447

Query: 172 LNGLSEFEVRRYTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGKGCFVAKLASFGL 231
              ++E  VR +TR I+ GL Y+H   N + +H DIK  N+L+  SG    V KLA FG+
Sbjct: 448 CGTMTESVVRNFTRHILSGLAYLH---NKKTVHRDIKGANLLVDASG----VVKLADFGM 507

Query: 232 AKNLTPEALCDQEISGSGSYISPELAR-------DGYLDWPADIWALGCVVFEMLTGKPA 291
           AK+LT +   D  + GS  +++PEL +       +  L +  DIW+LGC + EM TGKP 
Sbjct: 508 AKHLTGQR-ADLSLKGSPYWMAPELMQAVMQKDSNPDLAFAVDIWSLGCTIIEMFTGKPP 567

Query: 292 WSFEEAHNYMLGCYHRDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWLLIKHPFVCN 343
           WS  E    M     RD P IP  +S  G+DF++ C  R+P +R    +L++H F+ N
Sbjct: 568 WSEFEGAAAMFKVM-RDSPPIPESMSPEGKDFLRLCFQRNPAERPTASMLLEHRFLKN 609

[Close]

BLAST of Tan0010485 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 2.1e-29
Identity = 97/338 (28.70%), Postives = 150/338 (44.38%), Query Frame = 0


Query: 41  NNNNWNDNVKHNNGRFANFNV--------------EEF---HGSFWIGDCVFRKRGSGLI 100
           NNNN N+N  +NN    N N+              EE    H   W    +  + G G +
Sbjct: 125 NNNNNNNNNNNNNNNNNNNNIIQHTDFPSLIINEHEELISNHNIKWQKGQILGRGGYGSV 184

Query: 101 FLAKNKTPN-----------NPNSSQLIPTQIVVKSCFMEFSSSLQHEKEVLFNLAPCTN 160
           +L  NK              + NS   +   I+  S  +E   SL+H+           N
Sbjct: 185 YLGLNKDTGELFAVKQLEIVDINSDPKLKNMILSFSKEIEVMRSLRHD-----------N 244

Query: 161 VVRCYGDEITTTKSGEKFYNLLFEYCSGSSLRDYIINFGLNGLSEFEVRRYTRDIVHGLY 220
           +VR  G     T   + F ++  EY  G S+   +  FG    SE  ++ YT+ I+ GL 
Sbjct: 245 IVRYLG-----TSLDQSFLSVFLEYIPGGSISSLLGKFG--AFSENVIKVYTKQILQGLS 304

Query: 221 YMHCNANARYIHGDIKPRNILLSPSGKGCFVAKLASFGLAKNLTPEALCDQEISGSGSYI 280
           ++H N+    IH DIK  NIL+   G    + KL+ FG +K+ +      + + G+  ++
Sbjct: 305 FLHANS---IIHRDIKGANILIDTKG----IVKLSDFGCSKSFSGIVSQFKSMQGTPYWM 364

Query: 281 SPELARDGYLDWPADIWALGCVVFEMLTGKPAWSFEEAHNYMLGCYH----RDLPEIPYE 340
           +PE+ +       +DIW+LGCV+ EM T +P WS           YH      +P IP  
Sbjct: 365 APEVIKQTGHGRSSDIWSLGCVIVEMATAQPPWS--NITELAAVMYHIASSNSIPNIPSH 424

Query: 341 ISSAGRDFIKKCLIRSPYKRRPIWLLIKHPFVCNLNNS 347
           +S    DF+  C  R P +R     L+KHPF+ NL+++
Sbjct: 425 MSQEAFDFLNLCFKRDPKERPDANQLLKHPFIMNLDDN 435

[Close]

BLAST of Tan0010485 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 9.6e-27
Identity = 88/249 (35.34%), Postives = 131/249 (52.61%), Query Frame = 0


Query: 104 IVVKSCFMEFSSSLQHEKEVLFNLAPCTNVVRCYGDEITTT---KSGE-KFYNLLFEYCS 163
           + VKS     S  LQ E ++L +L     V+   G EIT      +GE   Y+LL EY  
Sbjct: 29  LAVKSAEFHRSEFLQREAKILSSL-NSPYVIGYRGCEITREPFHNNGEATTYSLLMEYAP 88

Query: 164 GSSLRDYIINFGLNGLSEFEVRRYTRDIVHGLYYMHCNANARYI-HGDIKPRNILLSPSG 223
             +L D     G   + E  V +YTR I+ GL Y+H   N++ I H DIK  N+L+  +G
Sbjct: 89  YGTLTDVATKNG-GFIDEARVVKYTRQILLGLEYIH---NSKGIAHCDIKGSNVLVGENG 148

Query: 224 KGCFVAKLASFGLAKNLTPEALCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEM 283
           +    AK+A FG AK + PE    + + G+ ++++PE AR       +DIWA+GC V EM
Sbjct: 149 E----AKIADFGCAKWVEPE--ITEPVRGTPAFMAPEAARGERQGKESDIWAVGCTVIEM 208

Query: 284 LTGKPAW---SFEEAHN--YMLGCYHRDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWL 343
           +TG   W    F +  +  Y +G Y  +LPE+P  ++   +DF+ KCL +   +R     
Sbjct: 209 VTGSQPWIGADFTDPVSVLYRVG-YLGELPELPCSLTEQAKDFLGKCLKKEATERWTASQ 265

[Close]

BLAST of Tan0010485 vs. NCBI nr
Match: XP_023516554.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 519.6 bits (1337), Expect = 2.1e-143
Identity = 251/342 (73.39%), Postives = 286/342 (83.63%), Query Frame = 0


Query: 1   MTKSCVASVVKEQQSHYFVNLHDFLKTKRGIVKCAKKTLWNNNNWNDNVKHNNGRFANFN 60
           MTKSCV S V  QQ H  VNLH+FLKTKRGIVKCAKK+  ++NNWN+NVKHN GRF NF 
Sbjct: 1   MTKSCVVSQV-NQQCHNIVNLHEFLKTKRGIVKCAKKS-QSSNNWNNNVKHNYGRFGNFA 60

Query: 61  VEEFHGSFWIGDCVFRKRGSGLIFLAKNKTPNNPNSSQLIPTQIVVKSCFMEFSSSLQHE 120
           +EE+HGSFWIGD + RK GSGL+FLAKNK+  N N       + +VKSC ME SSSLQ+E
Sbjct: 61  IEEYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSN------PEFLVKSCLMESSSSLQYE 120

Query: 121 KEVLFNLAPCTNVVRCYGDEITTTKSGEKFYNLLFEYCSGSSLRDYIINFGLNGLSEFEV 180
           KEVL NL PC+N+VRCYGDEIT  KSGE+ YN++FEYC GSSLRD+II FG NGL E  V
Sbjct: 121 KEVLRNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIV 180

Query: 181 RRYTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGK--GCFVAKLASFGLAKNLTPE 240
           R+YTRDIVHGL YMHCNA   YIHGDIKPRNILLSPS    GC VAKLASFGLA+ LTPE
Sbjct: 181 RQYTRDIVHGLCYMHCNAG--YIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPE 240

Query: 241 ALCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEMLTGKPAWSFEEAHNYMLGCY 300
            LCD+EISGSGSYI+PELA+DGYLDWPADIWALGCVV EMLTGKPAWSFE A+NY++ C 
Sbjct: 241 VLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDC- 300

Query: 301 HRDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWLLIKHPFV 341
           ++++PEIP +ISSAGRDF+K+CLIRSPYKRRP WLL+KHPFV
Sbjct: 301 NQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

[Close]

BLAST of Tan0010485 vs. NCBI nr
Match: KAG7023508.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 516.9 bits (1330), Expect = 1.3e-142
Identity = 250/342 (73.10%), Postives = 285/342 (83.33%), Query Frame = 0


Query: 1   MTKSCVASVVKEQQSHYFVNLHDFLKTKRGIVKCAKKTLWNNNNWNDNVKHNNGRFANFN 60
           MTKSCV S V  QQ H  VNLH+FLKTKRGIVKCAKK+  ++ NWN+NVKHN GRF NF 
Sbjct: 1   MTKSCVVSQV-NQQCHNIVNLHEFLKTKRGIVKCAKKS-QSSTNWNNNVKHNYGRFGNFA 60

Query: 61  VEEFHGSFWIGDCVFRKRGSGLIFLAKNKTPNNPNSSQLIPTQIVVKSCFMEFSSSLQHE 120
           +EE+HGSFWIGD + RK GSGL+FLAKNK+  N NS      + +VKSC ME SSSLQ+E
Sbjct: 61  IEEYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNS------EFLVKSCLMESSSSLQYE 120

Query: 121 KEVLFNLAPCTNVVRCYGDEITTTKSGEKFYNLLFEYCSGSSLRDYIINFGLNGLSEFEV 180
           KEVL NL PC+N+VRCYGDEIT  KSGE+ YN++FEYC GSSLRD+II FG NGL E  V
Sbjct: 121 KEVLCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIV 180

Query: 181 RRYTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGK--GCFVAKLASFGLAKNLTPE 240
           R+YTRDIV GL YMHCNA   YIHGDIKPRNILLSPS    GC VAKLASFGLA+ LTPE
Sbjct: 181 RKYTRDIVRGLCYMHCNAG--YIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPE 240

Query: 241 ALCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEMLTGKPAWSFEEAHNYMLGCY 300
            LCD+EISGSGSYI+PELA+DGYLDWPADIWALGCVV EMLTGKPAWSFE A+NY++ C 
Sbjct: 241 VLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNC- 300

Query: 301 HRDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWLLIKHPFV 341
           ++++PEIP +ISSAGRDF+K+CLIRSPYKRRP WLL+KHPFV
Sbjct: 301 NQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

[Close]

BLAST of Tan0010485 vs. NCBI nr
Match: KAG6589837.1 (Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 514.6 bits (1324), Expect = 6.6e-142
Identity = 249/342 (72.81%), Postives = 285/342 (83.33%), Query Frame = 0


Query: 1   MTKSCVASVVKEQQSHYFVNLHDFLKTKRGIVKCAKKTLWNNNNWNDNVKHNNGRFANFN 60
           MTKSCV S V  QQ H  VNLH+FLKTKRGIVKCAKK+  ++ NWN+NVKHN GRF NF 
Sbjct: 1   MTKSCVVSQV-NQQCHNIVNLHEFLKTKRGIVKCAKKS-QSSTNWNNNVKHNYGRFGNFA 60

Query: 61  VEEFHGSFWIGDCVFRKRGSGLIFLAKNKTPNNPNSSQLIPTQIVVKSCFMEFSSSLQHE 120
           +EE HGSFWIGD + RK GSGL+FLAKNK+  N NS      + +VKSC ME SSSLQ+E
Sbjct: 61  IEECHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNS------EFLVKSCLMESSSSLQYE 120

Query: 121 KEVLFNLAPCTNVVRCYGDEITTTKSGEKFYNLLFEYCSGSSLRDYIINFGLNGLSEFEV 180
           KEVL NL PC+N+VRCYGDEIT  KSGE+ YN++FEYC GSSLRD+II FG NGL E  V
Sbjct: 121 KEVLCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIV 180

Query: 181 RRYTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGK--GCFVAKLASFGLAKNLTPE 240
           R+YTRDIV GL Y+HCNA   YIHGDIKPRNILLSPS +  GC VAKLASFGLA+ LTPE
Sbjct: 181 RKYTRDIVRGLCYIHCNAG--YIHGDIKPRNILLSPSDRKGGCLVAKLASFGLARKLTPE 240

Query: 241 ALCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEMLTGKPAWSFEEAHNYMLGCY 300
            LCD+EISGSGSYI+PELA+DGYLDWPADIWALGCVV EMLTGKPAWSFE A+NY++ C 
Sbjct: 241 VLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNC- 300

Query: 301 HRDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWLLIKHPFV 341
           ++++PEIP +ISSAGRDF+K+CLIRSPYKRRP WLL+KHPFV
Sbjct: 301 NQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

[Close]

BLAST of Tan0010485 vs. NCBI nr
Match: XP_022960664.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata])

HSP 1 Score: 514.2 bits (1323), Expect = 8.6e-142
Identity = 249/342 (72.81%), Postives = 285/342 (83.33%), Query Frame = 0


Query: 1   MTKSCVASVVKEQQSHYFVNLHDFLKTKRGIVKCAKKTLWNNNNWNDNVKHNNGRFANFN 60
           MTKSCV S V  QQ H  VNLH+FLKTKRGIVKCAKK+  ++ NWN+NVKHN GRF NF 
Sbjct: 1   MTKSCVVSQV-NQQCHNIVNLHEFLKTKRGIVKCAKKS-QSSTNWNNNVKHNYGRFGNFA 60

Query: 61  VEEFHGSFWIGDCVFRKRGSGLIFLAKNKTPNNPNSSQLIPTQIVVKSCFMEFSSSLQHE 120
           +EE HGSFWIGD + R+ GSGL+FLAKNK+  N NS      + +VKSC ME SSSLQ+E
Sbjct: 61  IEECHGSFWIGDRLIRRSGSGLVFLAKNKSSTNSNS------EFLVKSCLMESSSSLQYE 120

Query: 121 KEVLFNLAPCTNVVRCYGDEITTTKSGEKFYNLLFEYCSGSSLRDYIINFGLNGLSEFEV 180
           KEVL NL PC+N+VRCYGDEIT +KSGE+ YN++FEYC GSSLRD+II FG NGL E  V
Sbjct: 121 KEVLCNLGPCSNLVRCYGDEITMSKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIV 180

Query: 181 RRYTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGK--GCFVAKLASFGLAKNLTPE 240
           R+YTRDIV GL YMHCNA   YIHGDIKPRNILLSPS    GC VAKLASFGLA+ LTPE
Sbjct: 181 RKYTRDIVRGLCYMHCNAG--YIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPE 240

Query: 241 ALCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEMLTGKPAWSFEEAHNYMLGCY 300
            LCD+EISGSGSYI+PELA+DGYLDWPADIWALGCVV EMLTGKPAWSFE A+NY++ C 
Sbjct: 241 VLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNC- 300

Query: 301 HRDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWLLIKHPFV 341
           ++++PEIP +ISSAGRDF+K+CLIRSPYKRRP WLL+KHPFV
Sbjct: 301 NQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

[Close]

BLAST of Tan0010485 vs. NCBI nr
Match: XP_038880300.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 463.0 bits (1190), Expect = 2.3e-126
Identity = 236/342 (69.01%), Postives = 265/342 (77.49%), Query Frame = 0


Query: 1   MTKSCVASVVKEQQSHYFVNLHDFLKTKRGIVKCAKKTLWNNNNWNDNVKHNNGRFA-NF 60
           MTKSCV S V  Q+SH FVNLH+FLKTKRGIVKCAKK+L   NNWNDNVKHN+ +F  +F
Sbjct: 1   MTKSCVVSQV-NQRSHNFVNLHEFLKTKRGIVKCAKKSL-RTNNWNDNVKHNSEKFGKDF 60

Query: 61  NVEEFHGSFWIGDCVFRKRGSGLIFLAKNKTPNNPNSSQLIPTQIVVKSCFMEFSSSLQH 120
            +EE+HGS W GD +FRK GS LIFLA+N+ P NPNS+  +PT+ +VKSC ME SSSLQH
Sbjct: 61  AIEEYHGSIWTGDHLFRKTGSSLIFLARNRAPTNPNSN--LPTEFMVKSCLMESSSSLQH 120

Query: 121 EKEVLFNLAPCTNVVRCYGDEITTTKSGEKFYNLLFEYCSGSSLRDYIINFGLNGLSEFE 180
           EKEVL NL   TN+VRCYGDEIT TKSGEK YN+LFEYC GSSLRD+I+ FG NGL E E
Sbjct: 121 EKEVLNNLGSFTNLVRCYGDEITITKSGEKVYNVLFEYCYGSSLRDHILKFGPNGLPEHE 180

Query: 181 VRRYTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGKGCFVAKLASFGLAKNLTPEA 240
           VRRYTRDIV+G+ YMHC  N RYIHGDIK RNIL+SP+G     AKLASFG A+ LT E 
Sbjct: 181 VRRYTRDIVYGVCYMHC--NGRYIHGDIKSRNILMSPTG-----AKLASFGWARKLTAEV 240

Query: 241 LCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEMLTGKPAWSFEEAHNYMLGCYH 300
           LC++EI GSG YISPELA DGYL WPADIWALGCVV EMLTGK   + EEA         
Sbjct: 241 LCEEEIRGSGPYISPELASDGYLGWPADIWALGCVVLEMLTGKSPRNNEEA--------- 300

Query: 301 RDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 342
               +I  +IS  GRDFIKKCLI SPYKRRP WLLIKHPFVC
Sbjct: 301 ---IQIINDISKEGRDFIKKCLITSPYKRRPSWLLIKHPFVC 319

[Close]

BLAST of Tan0010485 vs. ExPASy TrEMBL
Match: A0A6J1HBP5 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111461386 PE=4 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 4.2e-142
Identity = 249/342 (72.81%), Postives = 285/342 (83.33%), Query Frame = 0


Query: 1   MTKSCVASVVKEQQSHYFVNLHDFLKTKRGIVKCAKKTLWNNNNWNDNVKHNNGRFANFN 60
           MTKSCV S V  QQ H  VNLH+FLKTKRGIVKCAKK+  ++ NWN+NVKHN GRF NF 
Sbjct: 1   MTKSCVVSQV-NQQCHNIVNLHEFLKTKRGIVKCAKKS-QSSTNWNNNVKHNYGRFGNFA 60

Query: 61  VEEFHGSFWIGDCVFRKRGSGLIFLAKNKTPNNPNSSQLIPTQIVVKSCFMEFSSSLQHE 120
           +EE HGSFWIGD + R+ GSGL+FLAKNK+  N NS      + +VKSC ME SSSLQ+E
Sbjct: 61  IEECHGSFWIGDRLIRRSGSGLVFLAKNKSSTNSNS------EFLVKSCLMESSSSLQYE 120

Query: 121 KEVLFNLAPCTNVVRCYGDEITTTKSGEKFYNLLFEYCSGSSLRDYIINFGLNGLSEFEV 180
           KEVL NL PC+N+VRCYGDEIT +KSGE+ YN++FEYC GSSLRD+II FG NGL E  V
Sbjct: 121 KEVLCNLGPCSNLVRCYGDEITMSKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIV 180

Query: 181 RRYTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGK--GCFVAKLASFGLAKNLTPE 240
           R+YTRDIV GL YMHCNA   YIHGDIKPRNILLSPS    GC VAKLASFGLA+ LTPE
Sbjct: 181 RKYTRDIVRGLCYMHCNAG--YIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPE 240

Query: 241 ALCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEMLTGKPAWSFEEAHNYMLGCY 300
            LCD+EISGSGSYI+PELA+DGYLDWPADIWALGCVV EMLTGKPAWSFE A+NY++ C 
Sbjct: 241 VLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNC- 300

Query: 301 HRDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWLLIKHPFV 341
           ++++PEIP +ISSAGRDF+K+CLIRSPYKRRP WLL+KHPFV
Sbjct: 301 NQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

[Close]

BLAST of Tan0010485 vs. ExPASy TrEMBL
Match: A0A5D3D3K9 (Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1804G00170 PE=4 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 9.7e-123
Identity = 228/343 (66.47%), Postives = 259/343 (75.51%), Query Frame = 0


Query: 1   MTKSCVASVVKEQQSHYFVNLHDFLKTKRGIVKCAKKTLWNNNNWNDNVKHNNGRFA-NF 60
           MTKSCV S V+     + VNLH+FLKTKR I+K AKK+    NNWND +KHN  +F  +F
Sbjct: 1   MTKSCVVSRVQ-----FCVNLHEFLKTKRRILKIAKKSA-PTNNWNDTIKHNYEKFGEDF 60

Query: 61  NVEEFHGSFWIGDCVFRKRGSGLIFLA-KNKTPNNPNSSQLIPTQIVVKSCFMEFSSSLQ 120
            +EEFHGS W G  +FRK  S LIFLA KN+ P N NS+  +P + +VKS   E SSSL 
Sbjct: 61  TIEEFHGSIWSGVHLFRKTESTLIFLAKKNRAPIN-NSTSNLPEEFLVKSSLTESSSSLH 120

Query: 121 HEKEVLFNLAPCTNVVRCYGDEITTTKSGEKFYNLLFEYCSGSSLRDYIINFGLNGLSEF 180
           HEKEVL NL P  N+V CYGDEIT +KSGEK YN+ FEYCSGSSLRD+I  FG NGL E 
Sbjct: 121 HEKEVLSNLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEH 180

Query: 181 EVRRYTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGKGCFVAKLASFGLAKNLTPE 240
           EVR+YTRDIVHGLYYMHC  N RYIHGDIK RNILLS       +AKLASFGLA+ LT E
Sbjct: 181 EVRKYTRDIVHGLYYMHC--NGRYIHGDIKSRNILLSHG-----MAKLASFGLARKLTAE 240

Query: 241 ALCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEMLTGKPAWSFEEAHNYMLGCY 300
            +C++EISGSG Y SPELAR+GYL WPADIWALGCVV EM TGK AWSF +AH Y++ C 
Sbjct: 241 VICEEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDCN 300

Query: 301 HRDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 342
           +  +PEIP  IS  GRDFIKKCLIRSPYKRRP WLLIKHPFVC
Sbjct: 301 NEKIPEIPKNISREGRDFIKKCLIRSPYKRRPSWLLIKHPFVC 329

[Close]

BLAST of Tan0010485 vs. ExPASy TrEMBL
Match: A0A1S3B829 (mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656 GN=LOC103487049 PE=4 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 9.7e-123
Identity = 228/343 (66.47%), Postives = 259/343 (75.51%), Query Frame = 0


Query: 1   MTKSCVASVVKEQQSHYFVNLHDFLKTKRGIVKCAKKTLWNNNNWNDNVKHNNGRFA-NF 60
           MTKSCV S V+     + VNLH+FLKTKR I+K AKK+    NNWND +KHN  +F  +F
Sbjct: 1   MTKSCVVSRVQ-----FCVNLHEFLKTKRRILKIAKKSA-PTNNWNDTIKHNYEKFGEDF 60

Query: 61  NVEEFHGSFWIGDCVFRKRGSGLIFLA-KNKTPNNPNSSQLIPTQIVVKSCFMEFSSSLQ 120
            +EEFHGS W G  +FRK  S LIFLA KN+ P N NS+  +P + +VKS   E SSSL 
Sbjct: 61  TIEEFHGSIWSGVHLFRKTESTLIFLAKKNRAPIN-NSTSNLPEEFLVKSSLTESSSSLH 120

Query: 121 HEKEVLFNLAPCTNVVRCYGDEITTTKSGEKFYNLLFEYCSGSSLRDYIINFGLNGLSEF 180
           HEKEVL NL P  N+V CYGDEIT +KSGEK YN+ FEYCSGSSLRD+I  FG NGL E 
Sbjct: 121 HEKEVLSNLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEH 180

Query: 181 EVRRYTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGKGCFVAKLASFGLAKNLTPE 240
           EVR+YTRDIVHGLYYMHC  N RYIHGDIK RNILLS       +AKLASFGLA+ LT E
Sbjct: 181 EVRKYTRDIVHGLYYMHC--NGRYIHGDIKSRNILLSHG-----MAKLASFGLARKLTAE 240

Query: 241 ALCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEMLTGKPAWSFEEAHNYMLGCY 300
            +C++EISGSG Y SPELAR+GYL WPADIWALGCVV EM TGK AWSF +AH Y++ C 
Sbjct: 241 VICEEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDCN 300

Query: 301 HRDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 342
           +  +PEIP  IS  GRDFIKKCLIRSPYKRRP WLLIKHPFVC
Sbjct: 301 NEKIPEIPKNISREGRDFIKKCLIRSPYKRRPSWLLIKHPFVC 329

[Close]

BLAST of Tan0010485 vs. ExPASy TrEMBL
Match: A0A0A0LHV1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829110 PE=4 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 2.2e-122
Identity = 226/343 (65.89%), Postives = 261/343 (76.09%), Query Frame = 0


Query: 1   MTKSCVASVVKEQQSHYFVNLHDFLKTKRGIVKCAKKTLWNNNNWNDNVKHNNGRFA-NF 60
           MTKSCV S V+        NLH+FLKTKR I+K AKK+    NNWND +KHN  +F  +F
Sbjct: 1   MTKSCVVSQVQISG-----NLHEFLKTKRRILKIAKKSA-PRNNWNDTIKHNYEKFGEDF 60

Query: 61  NVEEFHGSFWIGDCVFRKRGSGLIFLA-KNKTPNNPNSSQLIPTQIVVKSCFMEFSSSLQ 120
            +EEFHGS W G  +FRK  S L+FLA KN+ P N NS+  +P + +VKS  ME SSSL+
Sbjct: 61  TIEEFHGSIWTGSLLFRKTESTLVFLAKKNRAPIN-NSNSNLPEEFLVKSSLMESSSSLR 120

Query: 121 HEKEVLFNLAPCTNVVRCYGDEITTTKSGEKFYNLLFEYCSGSSLRDYIINFGLNGLSEF 180
           HEKEVL NL P TN+V CYGDEIT TKSGE+ YN+ FEYCSGSSLR++I+ FG NGL + 
Sbjct: 121 HEKEVLSNLGPFTNLVDCYGDEITVTKSGEEVYNVFFEYCSGSSLRNHILKFGPNGLQDD 180

Query: 181 EVRRYTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGKGCFVAKLASFGLAKNLTPE 240
           EVRRYTRDIV GLYYMHC  N RYIHGDIK RNILLS       +AKLASFGLA+ LT E
Sbjct: 181 EVRRYTRDIVRGLYYMHC--NGRYIHGDIKSRNILLSHG-----MAKLASFGLARKLTAE 240

Query: 241 ALCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEMLTGKPAWSFEEAHNYMLGCY 300
            +C++EISGSG Y SPELAR+GYL WPADIWALGCVV EM TGK AWSFE+A+ Y++   
Sbjct: 241 VICEEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSN 300

Query: 301 HRDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 342
           +  +PEIP  IS  GRDFIKKCLIRSPYKRRPIWLLIKHPFVC
Sbjct: 301 NEKIPEIPKNISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 329

[Close]

BLAST of Tan0010485 vs. ExPASy TrEMBL
Match: A0A6J1JAH8 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=3661 GN=LOC111485031 PE=4 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 1.0e-103
Identity = 181/232 (78.02%), Postives = 204/232 (87.93%), Query Frame = 0


Query: 111 MEFSSSLQHEKEVLFNLAPCTNVVRCYGDEITTTKSGEKFYNLLFEYCSGSSLRDYIINF 170
           ME SSSLQ+EKEVL NL PC+N+VRCYGDEIT  KSGE+ YN++FEYC GSSLRD+II F
Sbjct: 1   MESSSSLQYEKEVLCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKF 60

Query: 171 GLNGLSEFEVRRYTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGK--GCFVAKLAS 230
           G NGL E  VR+YTRDIVHGL YMHC  NARYIHGDIKPRNILLSPS    GC VAKLAS
Sbjct: 61  GRNGLPEPIVRKYTRDIVHGLCYMHC--NARYIHGDIKPRNILLSPSDHKGGCLVAKLAS 120

Query: 231 FGLAKNLTPEALCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEMLTGKPAWSFE 290
           FGLA+ LTPE LCD+EISGSGSYI+PELA+DGYLDWPADIWALGCVV EMLTGKPAWSFE
Sbjct: 121 FGLARKLTPEVLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFE 180

Query: 291 EAHNYMLGCYHRDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWLLIKHPFV 341
            A+NY++ C ++++PEIP +ISSAGRDF+K+CLIRSPYKRRP WLL+KHPFV
Sbjct: 181 SAYNYLMDC-NQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 229

[Close]

BLAST of Tan0010485 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 184.1 bits (466), Expect = 1.9e-46
Identity = 103/252 (40.87%), Postives = 151/252 (59.92%), Query Frame = 0


Query: 95  NSSQLIPTQIVVKSCFMEFSSSLQHEKEVLFNLAPCTNVVRCYGDEITTTKSGEKFYNLL 154
           +SS+  P+ + VKS  +  S++L++E++VL +L  C+ +VRC+G E  T ++GE+ YNL 
Sbjct: 25  SSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDCSEIVRCFG-EGRTVENGEEIYNLF 84

Query: 155 FEYCSGSSLRDYIINFGLNGLSEFEVRRYTRDIVHGLYYMHCNANARYIHGDIKPRNILL 214
            EY SG SL D I + G   L EFEVRR+TR IV GL ++H N    + H DIK  N+L+
Sbjct: 85  LEYASGGSLADRIKSSG-EALPEFEVRRFTRSIVKGLCHIHGNG---FTHCDIKLENVLV 144

Query: 215 SPSGKGCFVAKLASFGLAKNLTPEALCDQEISGSGSYISPELARDGYLDWPADIWALGCV 274
              G      K++ FGLAK  + E     EI G+  Y++PE    G  + PADIWALGC 
Sbjct: 145 FGDGD----VKISDFGLAKRRSGEVCV--EIRGTPLYMAPESVNHGEFESPADIWALGCS 204

Query: 275 VFEMLTGKPAWSFEE-AHNYMLGCYHR-----DLPEIPYEISSAGRDFIKKCLIRSPYKR 334
           V EM +GK AW  E+   N ++    R     ++P IP E+S  G+DF+ KC +++  +R
Sbjct: 205 VVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIPVELSEEGKDFVSKCFVKNAAER 264

Query: 335 RPIWLLIKHPFV 341
               +L+ HPF+
Sbjct: 265 WTAEMLLDHPFL 265

[Close]

BLAST of Tan0010485 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 175.3 bits (443), Expect = 8.9e-44
Identity = 99/259 (38.22%), Postives = 141/259 (54.44%), Query Frame = 0


Query: 90  TPNNPNSSQLIPTQIVVKSCFMEFSSSLQHEKEVLFNLAPCTNVVRCYGDEITTTKSGEK 149
           T     +S   P  I VKS     ++SL +EK VL +L  C  ++RCYG++ +T ++GE+
Sbjct: 19  TATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIRCYGED-STVENGEE 78

Query: 150 FYNLLFEYCSGSSLRDYIINFGLNGLSEFEVRRYTRDIVHGLYYMHCNANARYIHGDIKP 209
            +NLL EY S  SL  Y+   G  GL E  VRR+T  ++ GL ++H      + H DIK 
Sbjct: 79  MHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIHAKG---FAHCDIKL 138

Query: 210 RNILLSPSGKGCFVAKLASFGLAKNLTPEALC---DQEISGSGSYISPELARDGYLDWPA 269
            NILL   G      K+A FGLA  +  +        EI G+  Y++PE   D      A
Sbjct: 139 ANILLFNDGS----VKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPECVNDNEYGSAA 198

Query: 270 DIWALGCVVFEMLTGKPAWSFEEAHNYM-----LGCYHRDLPEIPYEISSAGRDFIKKCL 329
           D+WALGC V EM +GK AWS +E  ++M     +G    +LP+IP  +S  G+DF+ KC 
Sbjct: 199 DVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIG-VGDELPKIPEMLSEEGKDFLSKCF 258

Query: 330 IRSPYKRRPIWLLIKHPFV 341
           ++ P KR    +L+ H FV
Sbjct: 259 VKDPAKRWTAEMLLNHSFV 268

[Close]

BLAST of Tan0010485 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 174.9 bits (442), Expect = 1.2e-43
Identity = 99/254 (38.98%), Postives = 140/254 (55.12%), Query Frame = 0


Query: 95  NSSQLIPTQIVVKSCFMEFSSSLQHEKEVLFNLA-PCTNVVRCYGDEITTTKSGEKFYNL 154
           N S   P  + VKS     ++SL +EK VL NL   C  +VRC+G++  T ++GE+ +NL
Sbjct: 25  NDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDDCNEIVRCFGED-RTVENGEEMHNL 84

Query: 155 LFEYCSGSSLRDYIINFGLNGLSEFEVRRYTRDIVHGLYYMHCNANARYIHGDIKPRNIL 214
             EY S  SL  Y+      G+ E  VRR+T  ++ GL ++H N    + H D+K  NIL
Sbjct: 85  FLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLRGLRHIHANG---FAHCDLKLGNIL 144

Query: 215 LSPSGKGCFVAKLASFGLAKNLTPEALCDQ--EISGSGSYISPELARDGYLDWPADIWAL 274
           L   G      K+A FGLAK +      +   +I G+  Y++PE   D       D+WAL
Sbjct: 145 LFGDG----AVKIADFGLAKRIGDLTALNYGVQIRGTPLYMAPESVNDNEYGSEGDVWAL 204

Query: 275 GCVVFEMLTGKPAWSFEEAHNYM-----LGCYHRDLPEIPYEISSAGRDFIKKCLIRSPY 334
           GCVV EM +GK AWS +E  N+M     +G    ++P IP E+S  GRDF+ KC ++ P 
Sbjct: 205 GCVVVEMFSGKTAWSLKEGSNFMSLLLRIG-VGDEVPMIPEELSEQGRDFLSKCFVKDPK 264

Query: 335 KRRPIWLLIKHPFV 341
           KR    +L+ HPFV
Sbjct: 265 KRWTAEMLLNHPFV 269

[Close]

BLAST of Tan0010485 vs. TAIR 10
Match: AT3G46140.1 (Protein kinase superfamily protein )

HSP 1 Score: 162.5 bits (410), Expect = 5.9e-40
Identity = 108/291 (37.11%), Postives = 148/291 (50.86%), Query Frame = 0


Query: 67  SFWIGDCVFRKRGSGLIFLAKNKTPNNPNSSQLIPTQIVVKSCFMEFSSSLQHEKEVLFN 126
           S WI      +   G ++LA +K            T + +KS  +  +SSL  E+ +L  
Sbjct: 94  SSWIKSEFLGRGSYGSVYLATSKKAKT-------KTTMAIKSAEISRASSLMDEERILTR 153

Query: 127 LAPCTNVVRCYGDEIT---TTKSGEKF-YNLLFEYCSGSSLRDYIINFGLNGLSEFEVRR 186
           L+    +VRCYG EI    T   GE+  YNL+ EYCSG SL D ++N  L GLSE +V+ 
Sbjct: 154 LS-SPFIVRCYGHEIAREETLFGGERTNYNLILEYCSGKSLFD-LVNDNLGGLSEKDVKL 213

Query: 187 YTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGK----GCFVAKLASFGLA--KNLT 246
             RDI++GL  +H    A  IH DIKP NI L+P         +VAK+  FGLA  K  +
Sbjct: 214 LARDILYGLDCIH---RANIIHCDIKPENIFLTPVENRIRPSGYVAKIGDFGLALEKGSS 273

Query: 247 PEALCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEMLTGKPAWSFEE-----AH 306
                     G+  Y+SPEL R G +D+  D WA GC V EMLTG+  W           
Sbjct: 274 EYEKASGHRRGTTRYMSPELIRHGIVDYAVDTWAFGCTVLEMLTGQQVWGEHSDLGSVDW 333

Query: 307 NYMLG--CYHRDLPEIPYEISSAGRDFIKKCLIRSPYKRRPIWLLIKHPFV 341
           + ++G  CY   +P IP  +S   + F+ +CL R P  R  I  L+ HPF+
Sbjct: 334 DILIGQSCY---IPYIPDWLSEEAQHFLSRCLKRDPASRWGIGALLNHPFL 369

[Close]

BLAST of Tan0010485 vs. TAIR 10
Match: AT3G45790.1 (Protein kinase superfamily protein )

HSP 1 Score: 157.5 bits (397), Expect = 1.9e-38
Identity = 104/276 (37.68%), Postives = 142/276 (51.45%), Query Frame = 0


Query: 67  SFWIGDCVFRKRGSGLIFLAKNKTPNNPNSSQLIPTQIVVKSCFMEFSSSLQHEKEVLFN 126
           S WI      +   G ++LA +K            T + +KS  +  +SSL  E+ +L  
Sbjct: 94  SSWIKSEFLGRGSYGSVYLATSKKAKT-------KTTMAIKSAEISRASSLMDEERILTR 153

Query: 127 LAPCTNVVRCYGDEIT---TTKSGEKF-YNLLFEYCSGSSLRDYIINFGLNGLSEFEVRR 186
           L+    +VRCYG EI    T   GE+  YNL+ EYCSG SL D ++N  L GLSE +V+ 
Sbjct: 154 LS-SPFIVRCYGHEIAREETLFGGERTNYNLILEYCSGKSLFD-LVNSNLGGLSEKDVKL 213

Query: 187 YTRDIVHGLYYMHCNANARYIHGDIKPRNILLSPSGK----GCFVAKLASFGLA--KNLT 246
             RDI++GL Y+H    A  IH DIKP NILL+P         +VAK+  FGLA  K  +
Sbjct: 214 LARDILYGLDYIH---RANIIHCDIKPENILLAPVENRIRPNGYVAKIGDFGLALEKGSS 273

Query: 247 PEALCDQEISGSGSYISPELARDGYLDWPADIWALGCVVFEMLTGKPAWSFEE-----AH 306
                     G+  Y+SPEL R G +D+  D WA GC V EMLTG+  W           
Sbjct: 274 EYEKASGHRRGTTRYMSPELIRHGIVDYAVDTWAFGCTVLEMLTGQQVWGEHSDLGSVDW 333

Query: 307 NYMLG--CYHRDLPEIPYEISSAGRDFIKKCLIRSP 326
           + ++G  CY   +P IP  +S   + F+ +CL R P
Sbjct: 334 DILIGQSCY---IPYIPDWLSEEAQHFLSRCLKRDP 354

The following BLAST results are available for this feature:

BLAST of Tan0010485 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	1.2e-42	38.22	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
O80888	2.2e-31	33.33	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q9C5H5	1.2e-29	35.71	Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702...	[more]
Q54R82	2.1e-29	28.70	Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=...	[more]
Q9ZVP5	9.6e-27	35.34	Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370...	[more]

BLAST of Tan0010485 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023516554.1	2.1e-143	73.39	mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pe...	[more]
KAG7023508.1	1.3e-142	73.10	Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper...	[more]
KAG6589837.1	6.6e-142	72.81	Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosper...	[more]
XP_022960664.1	8.6e-142	72.81	mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata]	[more]
XP_038880300.1	2.3e-126	69.01	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]

BLAST of Tan0010485 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBP5	4.2e-142	72.81	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=...	[more]
A0A5D3D3K9	9.7e-123	66.47	Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. ma...	[more]
A0A1S3B829	9.7e-123	66.47	mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656...	[more]
A0A0A0LHV1	2.2e-122	65.89	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829...	[more]
A0A6J1JAH8	1.0e-103	78.02	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=36...	[more]

BLAST of Tan0010485 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36950.1	1.9e-46	40.87	mitogen-activated protein kinase kinase kinase 21	[more]
AT3G50310.1	8.9e-44	38.22	mitogen-activated protein kinase kinase kinase 20	[more]
AT5G67080.1	1.2e-43	38.98	mitogen-activated protein kinase kinase kinase 19	[more]
AT3G46140.1	5.9e-40	37.11	Protein kinase superfamily protein	[more]
AT3G45790.1	1.9e-38	37.68	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 69..340 e-value: 1.6E-38 score: 144.0
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 114..340 e-value: 3.9E-37 score: 128.0
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 69..340 score: 33.884159
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 79..349 e-value: 6.3E-48 score: 165.6
None	No IPR available	PIRSR	PIRSR000606-51	PIRSR000606-51	coord: 150..328 e-value: 3.4E-17 score: 59.8
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 59..340
None	No IPR available	PANTHER	PTHR48011:SF55	SUBFAMILY NOT NAMED	coord: 59..340
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 202..214
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 80..346

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0010485.1	Tan0010485.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity