Tan0010424 (gene) Snake gourd v1

BLAST of Tan0010424 vs. ExPASy Swiss-Prot
Match: Q9FNL7 (Protein NRT1/ PTR FAMILY 5.2 OS=Arabidopsis thaliana OX=3702 GN=NPF5.2 PE=2 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 1.3e-230
Identity = 398/576 (69.10%), Postives = 476/576 (82.64%), Query Frame = 0

Query: 4   VEETGLDDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYLTK 63
           VEE G DDYTKDGTVDL+GNPV RS RGRWKACSF+VVYEVFERMAYYGIS+NL IY+T 
Sbjct: 3   VEEVG-DDYTKDGTVDLQGNPVRRSIRGRWKACSFVVVYEVFERMAYYGISSNLFIYMTT 62

Query: 64  KLHQGTVTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLAVS 123
           KLHQGTV S+NNVTNW GT W+ PILGAY+ DA LGRY TF+I+ AI F+GM +LTL+V+
Sbjct: 63  KLHQGTVKSSNNVTNWVGTSWLTPILGAYVGDALLGRYITFVISCAIYFSGMMVLTLSVT 122

Query: 124 VPSLKPPACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFDPK 183
           +P +KPP C     +NC++AS LQLAVFFGALY LA+GTGGTKPNISTIGADQFD FDPK
Sbjct: 123 IPGIKPPECSTTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDVFDPK 182

Query: 184 EKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGTPF 243
           EK QKLSFFNWWMFSIFFGTLFA T+LVY+QDNVGW+LGYGLPT+GLAISI IF+ GTPF
Sbjct: 183 EKTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLLGTPF 242

Query: 244 YRHKLPNGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSLRF 303
           YRHKLP GSPFT+MA VI+A+    + P+ +D    HEL   EY ++GAF I  T SLRF
Sbjct: 243 YRHKLPTGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTPSLRF 302

Query: 304 LNKAAIIRRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLFIK 363
           L++A++         N +W L CT T+VEETKQMLRM+P+L  TF+PS MLAQ +TLF+K
Sbjct: 303 LDRASL-----KTGTNHKWNL-CTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVK 362

Query: 364 QGTTLDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQRMG 423
           QGTTLDR V   F IPPASL+ FVT+SML+S+V+YDR+FVKI +++T NPRGITLLQRMG
Sbjct: 363 QGTTLDRKVTGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMG 422

Query: 424 IGMILHIMIMTIASRVERHRLYIARENGLV-QNGGQIPLTIFSLLPQFMLMGTADAFIEV 483
           IG+I HI+IM +AS  ER+RL +A ++GL+ Q G ++PLTIF+LLPQF+LMG AD+F+EV
Sbjct: 423 IGLIFHILIMIVASVTERYRLKVAADHGLIHQTGVKLPLTIFALLPQFVLMGMADSFLEV 482

Query: 484 AKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGNGWILNNLNASH 543
           AK+EFFYDQAPE+MKSLGTSYS TSL IGNF+SSFLLSTVS IT + G GWILNNLN S 
Sbjct: 483 AKLEFFYDQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGRGWILNNLNESR 542

Query: 544 LDYYYALIAVLSAINFFIYLLVSKFYVYKAEVSGSI 579
           LDYYY   AVL+ +NF ++L+V KFYVY+AEV+ S+
Sbjct: 543 LDYYYLFFAVLNLVNFVLFLVVVKFYVYRAEVTDSV 571

BLAST of Tan0010424 vs. ExPASy Swiss-Prot
Match: Q9FNL8 (Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 1.2e-218
Identity = 384/575 (66.78%), Postives = 462/575 (80.35%), Query Frame = 0

Query: 4   VEETGLDDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYLTK 63
           VEE G DDYTKDGTVDL+GN V RS  GRWKACSF+VVYEVFERMAYYGIS+NL+IY+T 
Sbjct: 3   VEEVG-DDYTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYMTT 62

Query: 64  KLHQGTVTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLAVS 123
           KLHQGTV S+NNVTNW GT W+ PILGAY+ADAH GRY TF+I+SAI   GM LLTL+VS
Sbjct: 63  KLHQGTVKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLSVS 122

Query: 124 VPSLKPPACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFDPK 183
           +P LKPP C     +NC++AS +QLAVFFGALY LA+GTGGTKPNISTIGADQFDEFDPK
Sbjct: 123 LPGLKPPKCSTANVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFDPK 182

Query: 184 EKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGTPF 243
           +K  K SFFNWWMFSIFFGT FATT+LVY+QDNVGW++GYGL T+GLA SI IF+ GT  
Sbjct: 183 DKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGTRL 242

Query: 244 YRHKLPNGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSLRF 303
           YRHKLP GSPFT+MA VI+A+      P+ +D  + +EL   EY  + AF I STSSLRF
Sbjct: 243 YRHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTSSLRF 302

Query: 304 LNKAAIIRRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLFIK 363
           LN+A+ ++ G +     +W+L CT+T+VEETKQML+M+P+L  TF+PS MLAQ  TLFIK
Sbjct: 303 LNRAS-LKTGSTH----KWRL-CTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIK 362

Query: 364 QGTTLDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQRMG 423
           QGTTLDR +  +F IPPASL  F T SML+S+VIYDR+FVK M++ T NPRGITLLQRMG
Sbjct: 363 QGTTLDRRLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMG 422

Query: 424 IGMILHIMIMTIASRVERHRLYIARENGLV-QNGGQIPLTIFSLLPQFMLMGTADAFIEV 483
           IGMILHI+IM IAS  ER+RL +A E+GL  Q    IPL+IF+LLPQ++LMG ADAFIE+
Sbjct: 423 IGMILHILIMIIASITERYRLKVAAEHGLTHQTAVPIPLSIFTLLPQYVLMGLADAFIEI 482

Query: 484 AKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGNGWILNNLNASH 543
           AK+EFFYDQAPE+MKSLGTSY+ TS+ +G F+SS LLS+VS IT + G GWI NNLN S 
Sbjct: 483 AKLEFFYDQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGRGWIQNNLNESR 542

Query: 544 LDYYYALIAVLSAINFFIYLLVSKFYVYKAEVSGS 578
           LD YY   AVL+ +NF ++L+V +FY Y+A+V+ S
Sbjct: 543 LDNYYMFFAVLNLLNFILFLVVIRFYEYRADVTQS 570

BLAST of Tan0010424 vs. ExPASy Swiss-Prot
Match: Q8VZR7 (Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2)

HSP 1 Score: 570.1 bits (1468), Expect = 2.9e-161
Identity = 289/564 (51.24%), Postives = 396/564 (70.21%), Query Frame = 0

Query: 12  YTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYLTKKLHQGTVT 71
           YT+DGTVDL+G PVL S  GRW+ACSF++ YE FERMA+YGI++NL+ YLTK+LH+ T++
Sbjct: 7   YTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDTIS 66

Query: 72  SANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLAVSVPSLKPPA 131
           S  NV NW+G VW+ PI GAYIAD+++GR+ TF  +S I   GM LLT+AV+V SL+P  
Sbjct: 67  SVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLRPTC 126

Query: 132 CLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFDPKEKAQKLSF 191
               +   C +AS LQ+  F+ +LY +A+G GGTKPNIST GADQFD +  +EK QK+SF
Sbjct: 127 ----ENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVSF 186

Query: 192 FNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGTPFYRHKLPNG 251
           FNWWMFS F G LFAT  LVYIQ+N+GW LGYG+PT+GL +S+++F  GTPFYRHK+   
Sbjct: 187 FNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIKT 246

Query: 252 SPFTR-MANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSLRFLNKAAII 311
               + +  V IAA  N  L  P+D  +L+ELD   Y   G  ++  T   RFL+KAAI 
Sbjct: 247 DNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAAI- 306

Query: 312 RRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLFIKQGTTLDR 371
                       ++ CTVT+VE  K++L +I I + T IPST+ AQ +TLF+KQGTTLDR
Sbjct: 307 --------KTSSRVPCTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDR 366

Query: 372 SVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQRMGIGMILHI 431
            +G++FQIP ASL +FVT+SMLLSV +YD+ FV  M++ T NPRGITLLQR+G+G  + I
Sbjct: 367 KIGSNFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQI 426

Query: 432 MIMTIASRVERHRLYIARENGLVQNGGQIPLTIFSLLPQFMLMGTADAFIEVAKIEFFYD 491
           + + IAS VE  R+ + +E  +      +P++IF LLPQ+ L+G  D F  +  +EFFYD
Sbjct: 427 VAIAIASAVEVKRMRVIKEFHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYD 486

Query: 492 QAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQ-NGNGWILNNLNASHLDYYYAL 551
           Q+PE M+SLGT++  + +G+GNFL+SFL++ +  IT++  G  WI NNLN S LDYYY  
Sbjct: 487 QSPEEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYGF 546

Query: 552 IAVLSAINFFIYLLVSKFYVYKAE 574
           + V+S +N  +++  +  YVYK++
Sbjct: 547 LVVISIVNMGLFVWAASKYVYKSD 557

BLAST of Tan0010424 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 6.4e-140
Identity = 265/569 (46.57%), Postives = 378/569 (66.43%), Query Frame = 0

Query: 10  DDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYLTKKLHQGT 69
           D YT+DGTVD+  NP  +   G WKAC FI+  E  ER+AYYG+ TNL+ YL  +L+QG 
Sbjct: 5   DVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGN 64

Query: 70  VTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLAVSVPSLKP 129
            T+ANNVTNW+GT ++ P++GA+IADA+LGRY T      I  +GM LLTL+ SVP LKP
Sbjct: 65  ATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKP 124

Query: 130 PACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFDPKEKAQKL 189
             C     D C   S  Q AVFF ALY++ALGTGG KP +S+ GADQFDE D  EK +K 
Sbjct: 125 GNC---NADTCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKS 184

Query: 190 SFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGTPFYRHKLP 249
           SFFNW+ FSI  G L A T+LV+IQ NVGW  G+G+PT+ + I++  F  G+ FYR + P
Sbjct: 185 SFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRP 244

Query: 250 NGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSLRFLNKAAI 309
            GSP TR+  VI+AA    S+ +P D   L E   DE   +G+ ++  T +L+F +KAA+
Sbjct: 245 GGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV 304

Query: 310 IRRGDSRDHND--EWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLFIKQGTT 369
             + DS    +   W+L C+VTQVEE K ++ ++P+     + +T+ +Q  T+F+ QG T
Sbjct: 305 ESQSDSIKDGEVNPWRL-CSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 364

Query: 370 LDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQRMGIGMI 429
           +D+ +G +F+IP ASL+ F T+S+L    +YD+  + + +++T+N RG T LQRMGIG++
Sbjct: 365 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 424

Query: 430 LHIMIMTIASRVERHRL-YIARENGLVQNGGQIPLTIFSLLPQFMLMGTADAFIEVAKIE 489
           + I  M  A  +E  RL Y+   N   Q   QI ++IF  +PQ++L+G A+ F  + ++E
Sbjct: 425 VSIFAMITAGVLEVVRLDYVKTHNAYDQK--QIHMSIFWQIPQYLLIGCAEVFTFIGQLE 484

Query: 490 FFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGN-GWILNNLNASHLDY 549
           FFYDQAP+ M+SL ++ S+T++ +GN+LS+ L++ V  IT +NG  GWI +NLN  HLDY
Sbjct: 485 FFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDY 544

Query: 550 YYALIAVLSAINFFIYLLVSKFYVYKAEV 575
           ++ L+A LS +NF +YL +SK Y YK  V
Sbjct: 545 FFYLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Tan0010424 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.1e-139
Identity = 250/563 (44.40%), Postives = 379/563 (67.32%), Query Frame = 0

Query: 12  YTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYLTKKLHQGTVT 71
           Y +DG+VD  GNP L+   G WKAC FI+  E  ER+AYYGI+ NLI YLT KLHQG V+
Sbjct: 24  YAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVS 83

Query: 72  SANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLAVSVPSLKPPA 131
           +A NVT W GT ++ P++GA +ADA+ GRY T    S I F GM  LTL+ SVP+LKP  
Sbjct: 84  AATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE 143

Query: 132 CLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFDPKEKAQKLSF 191
           C+    D C  A+  Q A+FFG LY++ALGTGG KP +S+ GADQFD+ D +E+ +K SF
Sbjct: 144 CI---GDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASF 203

Query: 192 FNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGTPFYRHKLPNG 251
           FNW+ FSI  G L ++++LV+IQ+N GW LG+G+PT+ + ++I  F  GTP YR + P G
Sbjct: 204 FNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGG 263

Query: 252 SPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSLRFLNKAAIIR 311
           SP TR++ V++A+    S+ +P D   L+E         G+ +I+ T   ++L+KAA+I 
Sbjct: 264 SPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 323

Query: 312 RGDSR--DHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLFIKQGTTLD 371
             +S+  D+++ W+L CTVTQVEE K ++RM PI     I S + AQ  T+F++QG  ++
Sbjct: 324 EEESKSGDYSNSWRL-CTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMN 383

Query: 372 RSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQRMGIGMILH 431
             +G+ FQ+PPA+L  F T S+++ V +YDR  V + +++T   +G T +QRMGIG+ + 
Sbjct: 384 CKIGS-FQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVS 443

Query: 432 IMIMTIASRVERHRLYIARENGLVQNGGQIPLTIFSLLPQFMLMGTADAFIEVAKIEFFY 491
           ++ M  A+ VE  RL++A + GLV++G  +P+++   +PQ+ ++G A+ F  + ++EFFY
Sbjct: 444 VLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFY 503

Query: 492 DQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNG-NGWILNNLNASHLDYYYA 551
           DQ+P+ M+SL ++ ++ +  +GN+LSS +L+ V++ T +NG  GWI +NLN+ HLDY++ 
Sbjct: 504 DQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYFFW 563

Query: 552 LIAVLSAINFFIYLLVSKFYVYK 572
           L+A LS +N  +Y   +  Y  K
Sbjct: 564 LLAGLSLVNMAVYFFSAARYKQK 581

BLAST of Tan0010424 vs. NCBI nr
Match: XP_022964042.1 (protein NRT1/ PTR FAMILY 5.2-like [Cucurbita moschata])

HSP 1 Score: 1015.4 bits (2624), Expect = 2.0e-292
Identity = 513/589 (87.10%), Postives = 550/589 (93.38%), Query Frame = 0

Query: 1   MAAVEETGLDDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIY 60
           MA+++ETGLD YT+DGTVDL GNPVLRS  GRWKACSFIVVYEVFERMAY+GISTNLI+Y
Sbjct: 1   MASLQETGLDRYTEDGTVDLHGNPVLRSKTGRWKACSFIVVYEVFERMAYHGISTNLILY 60

Query: 61  LTKKLHQGTVTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTL 120
           LTKKLHQGTV SANNVTNW+GTVW+MPILGAYIADAHLGRYRTFMIAS IC  GMGLLTL
Sbjct: 61  LTKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFMIASTICLMGMGLLTL 120

Query: 121 AVSVPSLKPPACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEF 180
           AVS+ SLKPP CLEV K NCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFD+F
Sbjct: 121 AVSISSLKPPPCLEVNKGNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDDF 180

Query: 181 DPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAG 240
            PKEKAQKLSFFNWWMFSIFFGTLFA T +VYIQDNVGWSLGYGLPTIGLA+SILIFVAG
Sbjct: 181 HPKEKAQKLSFFNWWMFSIFFGTLFANTGVVYIQDNVGWSLGYGLPTIGLAVSILIFVAG 240

Query: 241 TPFYRHKLPNGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSS 300
           TPFYRHKLP GSPFTRMANVI+AAA NW LPLPNDPK+LHEL+F++Y+ + AFRIDSTSS
Sbjct: 241 TPFYRHKLPGGSPFTRMANVIVAAARNWRLPLPNDPKELHELEFEKYSIERAFRIDSTSS 300

Query: 301 LRFLNKAAIIRRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTL 360
           LRFLNKAA IR G +    + WKL C+VTQVEETKQ+L+MIPIL+CTFIPSTMLAQTHTL
Sbjct: 301 LRFLNKAA-IRTGST----EPWKL-CSVTQVEETKQILKMIPILVCTFIPSTMLAQTHTL 360

Query: 361 FIKQGTTLDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQ 420
           FIKQGTTLDRSVG+HFQIPPASL+AFVTISMLLSVVIYDRIFVKIMQR TKNPRGITLLQ
Sbjct: 361 FIKQGTTLDRSVGSHFQIPPASLSAFVTISMLLSVVIYDRIFVKIMQRLTKNPRGITLLQ 420

Query: 421 RMGIGMILHIMIMTIASRVERHRLYIARENGLVQNGGQIPLTIFSLLPQFMLMGTADAFI 480
           RMGIGMILHIMIMTIASRVER RLY+ARENGLVQNGG+IPLTIF+LLPQFMLMGTADAFI
Sbjct: 421 RMGIGMILHIMIMTIASRVERRRLYVARENGLVQNGGEIPLTIFTLLPQFMLMGTADAFI 480

Query: 481 EVAKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGNGWILNNLNA 540
           EVAKIEFFYDQAPE+MKSLGTSY+MTSLG+GNFLSSFLLSTVSHIT  NGNGWILNNLNA
Sbjct: 481 EVAKIEFFYDQAPESMKSLGTSYAMTSLGVGNFLSSFLLSTVSHITKNNGNGWILNNLNA 540

Query: 541 SHLDYYYALIAVLSAINFFIYLLVSKFYVYKAEVSGSIKALADELLKDN 590
           SHLDYYYALIAVLSAINFF++L+VSK YVYKAEV GSI  LADEL + N
Sbjct: 541 SHLDYYYALIAVLSAINFFVFLIVSKLYVYKAEVCGSINVLADELKERN 583

BLAST of Tan0010424 vs. NCBI nr
Match: XP_022979366.1 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1011.9 bits (2615), Expect = 2.2e-291
Identity = 515/591 (87.14%), Postives = 553/591 (93.57%), Query Frame = 0

Query: 2   AAVEETGLDDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYL 61
           +A EE+G+DDYTKDGTVDLKGNPVLRS RGRWKACSFIVVYEVFERMAYYGISTNLII+L
Sbjct: 4   SAAEESGVDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNLIIFL 63

Query: 62  TKKLHQGTVTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLA 121
           TKKLHQGTV SANNVTNW+GTVW+MPILGAYIADAHLGRYRTF+IASAIC TGMGLLTLA
Sbjct: 64  TKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGLLTLA 123

Query: 122 VSVPSLKPPACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFD 181
           VS+PSLKPP CL+V K NCK AS LQLAVFFGALY+LALGTGGTKPNISTIGADQFDEF 
Sbjct: 124 VSLPSLKPPPCLDVNKGNCKPASTLQLAVFFGALYMLALGTGGTKPNISTIGADQFDEFH 183

Query: 182 PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT 241
           PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT
Sbjct: 184 PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT 243

Query: 242 PFYRHKLPNGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSL 301
           PFYRHKLP GSPFT+MA+VI+AA  NW LPLPNDPK+LHEL F+EY KQGAFRIDST SL
Sbjct: 244 PFYRHKLPTGSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFEEYAKQGAFRIDSTPSL 303

Query: 302 RFLNKAAIIRRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLF 361
           RFLNKAA IRRG S    D WKL CTVTQVEETKQMLRMIP+L+CTF+PSTMLAQTHTLF
Sbjct: 304 RFLNKAA-IRRGSS----DSWKL-CTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTLF 363

Query: 362 IKQGTTLDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQR 421
           IKQGTTLDRS+G+HFQIPPASLAAFVTISMLLSVVIYDR+FVKIMQR TKNPRGITLLQR
Sbjct: 364 IKQGTTLDRSIGSHFQIPPASLAAFVTISMLLSVVIYDRLFVKIMQRITKNPRGITLLQR 423

Query: 422 MGIGMILHIMIMTIASRVERHRLYIARENGLVQNG-GQ-IPLTIFSLLPQFMLMGTADAF 481
           MGIGMILH++IM IASRVERHRL +AR+NG V NG GQ +PLTIF+LLPQFML+G ADAF
Sbjct: 424 MGIGMILHVLIMIIASRVERHRLDVARQNGQVGNGSGQALPLTIFTLLPQFMLVGVADAF 483

Query: 482 IEVAKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGNGWILNNLN 541
            EVAKIEFFYDQAPE+MKSLGTSYSMTS+GIGNFLSSFLLSTVS IT+++GNGWI+NNLN
Sbjct: 484 TEVAKIEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKHGNGWIMNNLN 543

Query: 542 ASHLDYYYALIAVLSAINFFIYLLVSKFYVYKAEVSGSIKALADELLKDNK 591
           ASHLDYYYA +AVLSAINFF++LL+SKFYVYKAEVSGSIKALADE LKD K
Sbjct: 544 ASHLDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKALADE-LKDKK 587

BLAST of Tan0010424 vs. NCBI nr
Match: XP_023540279.1 (protein NRT1/ PTR FAMILY 5.2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1011.5 bits (2614), Expect = 2.9e-291
Identity = 513/589 (87.10%), Postives = 548/589 (93.04%), Query Frame = 0

Query: 1   MAAVEETGLDDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIY 60
           MA+++ETGLD YT+DGTVDL GNPVLRS  GRWKACSFIVVYEVFERMAY+GISTNLI+Y
Sbjct: 1   MASLQETGLDRYTEDGTVDLHGNPVLRSKTGRWKACSFIVVYEVFERMAYHGISTNLILY 60

Query: 61  LTKKLHQGTVTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTL 120
           LTKKLHQGTV SANNVTNW+GTVW+MPILGAYIADAHLGRYRTFMIAS IC  GMGLLTL
Sbjct: 61  LTKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFMIASTICLMGMGLLTL 120

Query: 121 AVSVPSLKPPACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEF 180
           AVS+ SLKPP CLEV K NCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFD+F
Sbjct: 121 AVSMSSLKPPPCLEVNKGNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDDF 180

Query: 181 DPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAG 240
            PKEKAQKLSFFNWWMFSIFFGTLFA T +VYIQDNVGWSLGYGLPTIGLAISILIFVAG
Sbjct: 181 HPKEKAQKLSFFNWWMFSIFFGTLFANTGVVYIQDNVGWSLGYGLPTIGLAISILIFVAG 240

Query: 241 TPFYRHKLPNGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSS 300
           TPFYRHKLP+GSPFTRMANVI+AAA NW LPLPNDPK+LHEL+F++Y+ + AFRIDSTSS
Sbjct: 241 TPFYRHKLPSGSPFTRMANVIVAAARNWRLPLPNDPKELHELEFEKYSIERAFRIDSTSS 300

Query: 301 LRFLNKAAIIRRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTL 360
           LRFLNKAA IR G +    + WKL C+VTQVEETKQ+L+MIPIL+CTFIPSTMLAQTHTL
Sbjct: 301 LRFLNKAA-IRTGST----EPWKL-CSVTQVEETKQILKMIPILVCTFIPSTMLAQTHTL 360

Query: 361 FIKQGTTLDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQ 420
           FIKQGTTLDRSVG+HFQIPPASL+AFVTISMLLSVVIYDRIFVKIMQR TKNPRGITLLQ
Sbjct: 361 FIKQGTTLDRSVGSHFQIPPASLSAFVTISMLLSVVIYDRIFVKIMQRLTKNPRGITLLQ 420

Query: 421 RMGIGMILHIMIMTIASRVERHRLYIARENGLVQNGGQIPLTIFSLLPQFMLMGTADAFI 480
           RMGIGMILH MIMTIASRVER RLY+ARENGLVQNGG+IPLTIFSLLPQFMLMGTADAF 
Sbjct: 421 RMGIGMILHTMIMTIASRVERRRLYVARENGLVQNGGEIPLTIFSLLPQFMLMGTADAFT 480

Query: 481 EVAKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGNGWILNNLNA 540
           EVAKIEFFYDQAPE+MKSLGTSYSMTSLG+GNFLSSFLLSTVSHIT  NGNGWILNNLNA
Sbjct: 481 EVAKIEFFYDQAPESMKSLGTSYSMTSLGVGNFLSSFLLSTVSHITKNNGNGWILNNLNA 540

Query: 541 SHLDYYYALIAVLSAINFFIYLLVSKFYVYKAEVSGSIKALADELLKDN 590
           SHLDYYYALIAVLSAINF ++L+VSK YVYKAEV GSI  LADEL + N
Sbjct: 541 SHLDYYYALIAVLSAINFIVFLIVSKLYVYKAEVCGSINVLADELKERN 583

BLAST of Tan0010424 vs. NCBI nr
Match: KAG6597346.1 (Protein NRT1/ PTR FAMILY 5.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1010.0 bits (2610), Expect = 8.5e-291
Identity = 509/588 (86.56%), Postives = 549/588 (93.37%), Query Frame = 0

Query: 2   AAVEETGLDDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYL 61
           A+++ETGLD YT+DGTVDL GNPVLRS  GRWKACSFIVVYEVFERMAY+GISTNLI+Y+
Sbjct: 3   ASLQETGLDGYTEDGTVDLHGNPVLRSKTGRWKACSFIVVYEVFERMAYHGISTNLILYM 62

Query: 62  TKKLHQGTVTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLA 121
           T+KLHQGTV SANNVTNW+GTVW+MPILGAYIADAHLGRYRTFMIAS IC  GMGLLTLA
Sbjct: 63  TRKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFMIASTICLMGMGLLTLA 122

Query: 122 VSVPSLKPPACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFD 181
           VS+ SLKPP CLEV K NCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFD+F 
Sbjct: 123 VSISSLKPPPCLEVNKGNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDDFH 182

Query: 182 PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT 241
           PKEKAQKLSFFNWWMFSIFFGTLFA T +VYIQDNVGWSLGYGLPTIGLA+SILIFVAGT
Sbjct: 183 PKEKAQKLSFFNWWMFSIFFGTLFANTGVVYIQDNVGWSLGYGLPTIGLAVSILIFVAGT 242

Query: 242 PFYRHKLPNGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSL 301
           PFYRHKLP GSPFTRMANVI+AAA NW LPLPNDPK+LHEL+F++Y+ + AFRIDSTSSL
Sbjct: 243 PFYRHKLPGGSPFTRMANVIVAAARNWRLPLPNDPKELHELEFEKYSIERAFRIDSTSSL 302

Query: 302 RFLNKAAIIRRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLF 361
           RFLNKAA IR G +    + WKL C+VTQVEETKQ+L+MIPIL+CTFIPSTMLAQTHTLF
Sbjct: 303 RFLNKAA-IRTGST----EPWKL-CSVTQVEETKQILKMIPILVCTFIPSTMLAQTHTLF 362

Query: 362 IKQGTTLDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQR 421
           IKQGTTLDRSVG+HFQIPPASL+AFVTISMLLSVVIYDRIFVKIMQR TKNPRGITLLQR
Sbjct: 363 IKQGTTLDRSVGSHFQIPPASLSAFVTISMLLSVVIYDRIFVKIMQRLTKNPRGITLLQR 422

Query: 422 MGIGMILHIMIMTIASRVERHRLYIARENGLVQNGGQIPLTIFSLLPQFMLMGTADAFIE 481
           MGIGMILHIMIMTIASRVER RLY+ARENGLVQNGG+IPLTIF+LLPQF+LMGTADAFIE
Sbjct: 423 MGIGMILHIMIMTIASRVERRRLYVARENGLVQNGGEIPLTIFTLLPQFILMGTADAFIE 482

Query: 482 VAKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGNGWILNNLNAS 541
           VAKIEFFYDQAPE+MKSLGTSY+MTSLG+GNFLSSFLLSTVSHIT  NGNGWILNNLNAS
Sbjct: 483 VAKIEFFYDQAPESMKSLGTSYAMTSLGVGNFLSSFLLSTVSHITKNNGNGWILNNLNAS 542

Query: 542 HLDYYYALIAVLSAINFFIYLLVSKFYVYKAEVSGSIKALADELLKDN 590
           HLDYYYALIAVLSAINFF++L+VSK YVYKAEV GSI  LADEL + N
Sbjct: 543 HLDYYYALIAVLSAINFFVFLIVSKLYVYKAEVCGSINVLADELKERN 584

BLAST of Tan0010424 vs. NCBI nr
Match: XP_022924429.1 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1008.8 bits (2607), Expect = 1.9e-290
Identity = 512/591 (86.63%), Postives = 549/591 (92.89%), Query Frame = 0

Query: 2   AAVEETGLDDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYL 61
           +A EE+G+DDYTKDGTVDLKGNPVLRS RGRWKACSFIVVYEVFERMAYYGISTNLII+L
Sbjct: 4   SAAEESGVDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNLIIFL 63

Query: 62  TKKLHQGTVTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLA 121
           TKKLHQGTV SANNVTNW+GTVW+MPILGAYIADAHLGRYRTF+IASAIC TGMGLLTLA
Sbjct: 64  TKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGLLTLA 123

Query: 122 VSVPSLKPPACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFD 181
           VS+PSLKPP CL+V K NCK AS LQLAVFFGALY+LALGTGGTKPNISTIGADQFDEF 
Sbjct: 124 VSLPSLKPPPCLDVNKGNCKPASTLQLAVFFGALYMLALGTGGTKPNISTIGADQFDEFH 183

Query: 182 PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT 241
           PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT
Sbjct: 184 PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT 243

Query: 242 PFYRHKLPNGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSL 301
           PFYRHKLP GSPFT+MA+VI+AA  NW LPLPNDPK+LHEL F+EY KQGAFRIDST SL
Sbjct: 244 PFYRHKLPTGSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFEEYAKQGAFRIDSTPSL 303

Query: 302 RFLNKAAIIRRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLF 361
           RFLNKAA IRRG S    D WKL CTVTQVEETKQMLRMIP+L+CTF+PSTMLAQTHTLF
Sbjct: 304 RFLNKAA-IRRGSS----DSWKL-CTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTLF 363

Query: 362 IKQGTTLDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQR 421
           IKQGTTLDRS+G+HFQIPPASLAAFVTISMLLSVVIYDR+FVKIMQR TKNPRGITLLQR
Sbjct: 364 IKQGTTLDRSIGSHFQIPPASLAAFVTISMLLSVVIYDRLFVKIMQRITKNPRGITLLQR 423

Query: 422 MGIGMILHIMIMTIASRVERHRLYIARENGLVQN--GGQIPLTIFSLLPQFMLMGTADAF 481
           MGIGMILH++IM IASRVERHRL +AR+NG V N  G  +PLTIF+LLPQFML+G ADAF
Sbjct: 424 MGIGMILHVLIMIIASRVERHRLDVARQNGQVGNASGQALPLTIFTLLPQFMLVGVADAF 483

Query: 482 IEVAKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGNGWILNNLN 541
            EVAKIEFFYDQAPE+MKSLGTSYSMTS+GIGNFLSSFLLSTVS IT++ GNGWI+NNLN
Sbjct: 484 TEVAKIEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKRGNGWIMNNLN 543

Query: 542 ASHLDYYYALIAVLSAINFFIYLLVSKFYVYKAEVSGSIKALADELLKDNK 591
           ASHLDYYYA +AVLSAINFF++LL+SKFYVYKAEVSGSIK LADE LKD K
Sbjct: 544 ASHLDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKVLADE-LKDKK 587

BLAST of Tan0010424 vs. ExPASy TrEMBL
Match: A0A6J1HGR6 (protein NRT1/ PTR FAMILY 5.2-like OS=Cucurbita moschata OX=3662 GN=LOC111464156 PE=3 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 9.8e-293
Identity = 513/589 (87.10%), Postives = 550/589 (93.38%), Query Frame = 0

Query: 1   MAAVEETGLDDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIY 60
           MA+++ETGLD YT+DGTVDL GNPVLRS  GRWKACSFIVVYEVFERMAY+GISTNLI+Y
Sbjct: 1   MASLQETGLDRYTEDGTVDLHGNPVLRSKTGRWKACSFIVVYEVFERMAYHGISTNLILY 60

Query: 61  LTKKLHQGTVTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTL 120
           LTKKLHQGTV SANNVTNW+GTVW+MPILGAYIADAHLGRYRTFMIAS IC  GMGLLTL
Sbjct: 61  LTKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFMIASTICLMGMGLLTL 120

Query: 121 AVSVPSLKPPACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEF 180
           AVS+ SLKPP CLEV K NCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFD+F
Sbjct: 121 AVSISSLKPPPCLEVNKGNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDDF 180

Query: 181 DPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAG 240
            PKEKAQKLSFFNWWMFSIFFGTLFA T +VYIQDNVGWSLGYGLPTIGLA+SILIFVAG
Sbjct: 181 HPKEKAQKLSFFNWWMFSIFFGTLFANTGVVYIQDNVGWSLGYGLPTIGLAVSILIFVAG 240

Query: 241 TPFYRHKLPNGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSS 300
           TPFYRHKLP GSPFTRMANVI+AAA NW LPLPNDPK+LHEL+F++Y+ + AFRIDSTSS
Sbjct: 241 TPFYRHKLPGGSPFTRMANVIVAAARNWRLPLPNDPKELHELEFEKYSIERAFRIDSTSS 300

Query: 301 LRFLNKAAIIRRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTL 360
           LRFLNKAA IR G +    + WKL C+VTQVEETKQ+L+MIPIL+CTFIPSTMLAQTHTL
Sbjct: 301 LRFLNKAA-IRTGST----EPWKL-CSVTQVEETKQILKMIPILVCTFIPSTMLAQTHTL 360

Query: 361 FIKQGTTLDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQ 420
           FIKQGTTLDRSVG+HFQIPPASL+AFVTISMLLSVVIYDRIFVKIMQR TKNPRGITLLQ
Sbjct: 361 FIKQGTTLDRSVGSHFQIPPASLSAFVTISMLLSVVIYDRIFVKIMQRLTKNPRGITLLQ 420

Query: 421 RMGIGMILHIMIMTIASRVERHRLYIARENGLVQNGGQIPLTIFSLLPQFMLMGTADAFI 480
           RMGIGMILHIMIMTIASRVER RLY+ARENGLVQNGG+IPLTIF+LLPQFMLMGTADAFI
Sbjct: 421 RMGIGMILHIMIMTIASRVERRRLYVARENGLVQNGGEIPLTIFTLLPQFMLMGTADAFI 480

Query: 481 EVAKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGNGWILNNLNA 540
           EVAKIEFFYDQAPE+MKSLGTSY+MTSLG+GNFLSSFLLSTVSHIT  NGNGWILNNLNA
Sbjct: 481 EVAKIEFFYDQAPESMKSLGTSYAMTSLGVGNFLSSFLLSTVSHITKNNGNGWILNNLNA 540

Query: 541 SHLDYYYALIAVLSAINFFIYLLVSKFYVYKAEVSGSIKALADELLKDN 590
           SHLDYYYALIAVLSAINFF++L+VSK YVYKAEV GSI  LADEL + N
Sbjct: 541 SHLDYYYALIAVLSAINFFVFLIVSKLYVYKAEVCGSINVLADELKERN 583

BLAST of Tan0010424 vs. ExPASy TrEMBL
Match: A0A6J1IQK5 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479112 PE=3 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 1.1e-291
Identity = 515/591 (87.14%), Postives = 553/591 (93.57%), Query Frame = 0

Query: 2   AAVEETGLDDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYL 61
           +A EE+G+DDYTKDGTVDLKGNPVLRS RGRWKACSFIVVYEVFERMAYYGISTNLII+L
Sbjct: 4   SAAEESGVDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNLIIFL 63

Query: 62  TKKLHQGTVTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLA 121
           TKKLHQGTV SANNVTNW+GTVW+MPILGAYIADAHLGRYRTF+IASAIC TGMGLLTLA
Sbjct: 64  TKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGLLTLA 123

Query: 122 VSVPSLKPPACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFD 181
           VS+PSLKPP CL+V K NCK AS LQLAVFFGALY+LALGTGGTKPNISTIGADQFDEF 
Sbjct: 124 VSLPSLKPPPCLDVNKGNCKPASTLQLAVFFGALYMLALGTGGTKPNISTIGADQFDEFH 183

Query: 182 PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT 241
           PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT
Sbjct: 184 PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT 243

Query: 242 PFYRHKLPNGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSL 301
           PFYRHKLP GSPFT+MA+VI+AA  NW LPLPNDPK+LHEL F+EY KQGAFRIDST SL
Sbjct: 244 PFYRHKLPTGSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFEEYAKQGAFRIDSTPSL 303

Query: 302 RFLNKAAIIRRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLF 361
           RFLNKAA IRRG S    D WKL CTVTQVEETKQMLRMIP+L+CTF+PSTMLAQTHTLF
Sbjct: 304 RFLNKAA-IRRGSS----DSWKL-CTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTLF 363

Query: 362 IKQGTTLDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQR 421
           IKQGTTLDRS+G+HFQIPPASLAAFVTISMLLSVVIYDR+FVKIMQR TKNPRGITLLQR
Sbjct: 364 IKQGTTLDRSIGSHFQIPPASLAAFVTISMLLSVVIYDRLFVKIMQRITKNPRGITLLQR 423

Query: 422 MGIGMILHIMIMTIASRVERHRLYIARENGLVQNG-GQ-IPLTIFSLLPQFMLMGTADAF 481
           MGIGMILH++IM IASRVERHRL +AR+NG V NG GQ +PLTIF+LLPQFML+G ADAF
Sbjct: 424 MGIGMILHVLIMIIASRVERHRLDVARQNGQVGNGSGQALPLTIFTLLPQFMLVGVADAF 483

Query: 482 IEVAKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGNGWILNNLN 541
            EVAKIEFFYDQAPE+MKSLGTSYSMTS+GIGNFLSSFLLSTVS IT+++GNGWI+NNLN
Sbjct: 484 TEVAKIEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKHGNGWIMNNLN 543

Query: 542 ASHLDYYYALIAVLSAINFFIYLLVSKFYVYKAEVSGSIKALADELLKDNK 591
           ASHLDYYYA +AVLSAINFF++LL+SKFYVYKAEVSGSIKALADE LKD K
Sbjct: 544 ASHLDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKALADE-LKDKK 587

BLAST of Tan0010424 vs. ExPASy TrEMBL
Match: A0A6J1E956 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431935 PE=3 SV=1)

HSP 1 Score: 1008.8 bits (2607), Expect = 9.1e-291
Identity = 512/591 (86.63%), Postives = 549/591 (92.89%), Query Frame = 0

Query: 2   AAVEETGLDDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYL 61
           +A EE+G+DDYTKDGTVDLKGNPVLRS RGRWKACSFIVVYEVFERMAYYGISTNLII+L
Sbjct: 4   SAAEESGVDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNLIIFL 63

Query: 62  TKKLHQGTVTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLA 121
           TKKLHQGTV SANNVTNW+GTVW+MPILGAYIADAHLGRYRTF+IASAIC TGMGLLTLA
Sbjct: 64  TKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGLLTLA 123

Query: 122 VSVPSLKPPACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFD 181
           VS+PSLKPP CL+V K NCK AS LQLAVFFGALY+LALGTGGTKPNISTIGADQFDEF 
Sbjct: 124 VSLPSLKPPPCLDVNKGNCKPASTLQLAVFFGALYMLALGTGGTKPNISTIGADQFDEFH 183

Query: 182 PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT 241
           PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT
Sbjct: 184 PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT 243

Query: 242 PFYRHKLPNGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSL 301
           PFYRHKLP GSPFT+MA+VI+AA  NW LPLPNDPK+LHEL F+EY KQGAFRIDST SL
Sbjct: 244 PFYRHKLPTGSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFEEYAKQGAFRIDSTPSL 303

Query: 302 RFLNKAAIIRRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLF 361
           RFLNKAA IRRG S    D WKL CTVTQVEETKQMLRMIP+L+CTF+PSTMLAQTHTLF
Sbjct: 304 RFLNKAA-IRRGSS----DSWKL-CTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTLF 363

Query: 362 IKQGTTLDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQR 421
           IKQGTTLDRS+G+HFQIPPASLAAFVTISMLLSVVIYDR+FVKIMQR TKNPRGITLLQR
Sbjct: 364 IKQGTTLDRSIGSHFQIPPASLAAFVTISMLLSVVIYDRLFVKIMQRITKNPRGITLLQR 423

Query: 422 MGIGMILHIMIMTIASRVERHRLYIARENGLVQN--GGQIPLTIFSLLPQFMLMGTADAF 481
           MGIGMILH++IM IASRVERHRL +AR+NG V N  G  +PLTIF+LLPQFML+G ADAF
Sbjct: 424 MGIGMILHVLIMIIASRVERHRLDVARQNGQVGNASGQALPLTIFTLLPQFMLVGVADAF 483

Query: 482 IEVAKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGNGWILNNLN 541
            EVAKIEFFYDQAPE+MKSLGTSYSMTS+GIGNFLSSFLLSTVS IT++ GNGWI+NNLN
Sbjct: 484 TEVAKIEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKRGNGWIMNNLN 543

Query: 542 ASHLDYYYALIAVLSAINFFIYLLVSKFYVYKAEVSGSIKALADELLKDNK 591
           ASHLDYYYA +AVLSAINFF++LL+SKFYVYKAEVSGSIK LADE LKD K
Sbjct: 544 ASHLDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKVLADE-LKDKK 587

BLAST of Tan0010424 vs. ExPASy TrEMBL
Match: A0A5D3D248 (Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001890 PE=3 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 1.8e-286
Identity = 502/589 (85.23%), Postives = 546/589 (92.70%), Query Frame = 0

Query: 2   AAVEETGLDDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYL 61
           +A EETGLD YTKDGTVDLKGNPVLRS RGRWKACSFIVVYEVFERMAYYGISTNLIIYL
Sbjct: 5   SAAEETGLDGYTKDGTVDLKGNPVLRSQRGRWKACSFIVVYEVFERMAYYGISTNLIIYL 64

Query: 62  TKKLHQGTVTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLA 121
           TKKLHQGTVTSANNVTNW+GTVW+MPILGAY+ADAHLGRYRTF+IASAIC TGMG+LTLA
Sbjct: 65  TKKLHQGTVTSANNVTNWSGTVWIMPILGAYVADAHLGRYRTFLIASAICLTGMGILTLA 124

Query: 122 VSVPSLKPPACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFD 181
           VS+PSLKPP CL+V K+NC++AS LQL VFFGALY+LALGTGGTKPNIST+GADQFDEFD
Sbjct: 125 VSLPSLKPPPCLDVNKENCEKASTLQLVVFFGALYLLALGTGGTKPNISTMGADQFDEFD 184

Query: 182 PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT 241
           PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISI+IF+AGT
Sbjct: 185 PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISIVIFLAGT 244

Query: 242 PFYRHKLPNGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSL 301
           PFYRHK+PNGSPFT M +VI+ A  NW +P+PNDPK+LHEL+F++Y+KQG FRIDSTSS 
Sbjct: 245 PFYRHKIPNGSPFTTMVSVIVGAIRNWRIPVPNDPKELHELEFEDYSKQGTFRIDSTSSF 304

Query: 302 RFLNKAAIIRRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLF 361
           RFLNKAA IRRG        WKL CTVTQVEETKQML+MIPIL+CTFIPSTMLAQTHTLF
Sbjct: 305 RFLNKAA-IRRG-------SWKL-CTVTQVEETKQMLKMIPILICTFIPSTMLAQTHTLF 364

Query: 362 IKQGTTLDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQR 421
           IKQGTTLDRSVG+HF+IP ASLA+FVTISMLLSVVIYDR+FVKIMQR TKNPRGITLLQR
Sbjct: 365 IKQGTTLDRSVGSHFKIPAASLASFVTISMLLSVVIYDRVFVKIMQRITKNPRGITLLQR 424

Query: 422 MGIGMILHIMIMTIASRVERHRLYIARENGLVQNGGQIPLTIFSLLPQFMLMGTADAFIE 481
           MGIGMILHI+IMTIASRVE HRL +ARENG  Q    +PLTIF+LLPQFMLMGTADAF+E
Sbjct: 425 MGIGMILHILIMTIASRVETHRLKVARENGSPQ---VLPLTIFTLLPQFMLMGTADAFME 484

Query: 482 VAKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGNGWILNNLNAS 541
           VAKIEFFYDQAPE MKSLGTS+SMTSLGIGNFLS+FLLSTVSHIT +NGNGW+LNNLN+S
Sbjct: 485 VAKIEFFYDQAPETMKSLGTSFSMTSLGIGNFLSTFLLSTVSHITIKNGNGWVLNNLNSS 544

Query: 542 HLDYYYALIAVLSAINFFIYLLVSKFYVYKAEVSGSIKALADELLKDNK 591
           HLDYYYALIAVLS INFF++L+VSKFYVYKAEVS SIK LADE LKD K
Sbjct: 545 HLDYYYALIAVLSTINFFVFLIVSKFYVYKAEVSTSIKVLADE-LKDKK 580

BLAST of Tan0010424 vs. ExPASy TrEMBL
Match: A0A1S3AW47 (protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo OX=3656 GN=LOC103483562 PE=3 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 1.8e-286
Identity = 502/589 (85.23%), Postives = 546/589 (92.70%), Query Frame = 0

Query: 2   AAVEETGLDDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYL 61
           +A EETGLD YTKDGTVDLKGNPVLRS RGRWKACSFIVVYEVFERMAYYGISTNLIIYL
Sbjct: 5   SAAEETGLDGYTKDGTVDLKGNPVLRSQRGRWKACSFIVVYEVFERMAYYGISTNLIIYL 64

Query: 62  TKKLHQGTVTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLA 121
           TKKLHQGTVTSANNVTNW+GTVW+MPILGAY+ADAHLGRYRTF+IASAIC TGMG+LTLA
Sbjct: 65  TKKLHQGTVTSANNVTNWSGTVWIMPILGAYVADAHLGRYRTFLIASAICLTGMGILTLA 124

Query: 122 VSVPSLKPPACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFD 181
           VS+PSLKPP CL+V K+NC++AS LQL VFFGALY+LALGTGGTKPNIST+GADQFDEFD
Sbjct: 125 VSLPSLKPPPCLDVNKENCEKASTLQLVVFFGALYLLALGTGGTKPNISTMGADQFDEFD 184

Query: 182 PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGT 241
           PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISI+IF+AGT
Sbjct: 185 PKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISIVIFLAGT 244

Query: 242 PFYRHKLPNGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSL 301
           PFYRHK+PNGSPFT M +VI+ A  NW +P+PNDPK+LHEL+F++Y+KQG FRIDSTSS 
Sbjct: 245 PFYRHKIPNGSPFTTMVSVIVGAIRNWRIPVPNDPKELHELEFEDYSKQGTFRIDSTSSF 304

Query: 302 RFLNKAAIIRRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLF 361
           RFLNKAA IRRG        WKL CTVTQVEETKQML+MIPIL+CTFIPSTMLAQTHTLF
Sbjct: 305 RFLNKAA-IRRG-------SWKL-CTVTQVEETKQMLKMIPILICTFIPSTMLAQTHTLF 364

Query: 362 IKQGTTLDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQR 421
           IKQGTTLDRSVG+HF+IP ASLA+FVTISMLLSVVIYDR+FVKIMQR TKNPRGITLLQR
Sbjct: 365 IKQGTTLDRSVGSHFKIPAASLASFVTISMLLSVVIYDRVFVKIMQRITKNPRGITLLQR 424

Query: 422 MGIGMILHIMIMTIASRVERHRLYIARENGLVQNGGQIPLTIFSLLPQFMLMGTADAFIE 481
           MGIGMILHI+IMTIASRVE HRL +ARENG  Q    +PLTIF+LLPQFMLMGTADAF+E
Sbjct: 425 MGIGMILHILIMTIASRVETHRLKVARENGSPQ---VLPLTIFTLLPQFMLMGTADAFME 484

Query: 482 VAKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGNGWILNNLNAS 541
           VAKIEFFYDQAPE MKSLGTS+SMTSLGIGNFLS+FLLSTVSHIT +NGNGW+LNNLN+S
Sbjct: 485 VAKIEFFYDQAPETMKSLGTSFSMTSLGIGNFLSTFLLSTVSHITIKNGNGWVLNNLNSS 544

Query: 542 HLDYYYALIAVLSAINFFIYLLVSKFYVYKAEVSGSIKALADELLKDNK 591
           HLDYYYALIAVLS INFF++L+VSKFYVYKAEVS SIK LADE LKD K
Sbjct: 545 HLDYYYALIAVLSTINFFVFLIVSKFYVYKAEVSTSIKVLADE-LKDKK 580

BLAST of Tan0010424 vs. TAIR 10
Match: AT5G46050.1 (peptide transporter 3 )

HSP 1 Score: 800.4 bits (2066), Expect = 9.5e-232
Identity = 398/576 (69.10%), Postives = 476/576 (82.64%), Query Frame = 0

Query: 4   VEETGLDDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYLTK 63
           VEE G DDYTKDGTVDL+GNPV RS RGRWKACSF+VVYEVFERMAYYGIS+NL IY+T 
Sbjct: 3   VEEVG-DDYTKDGTVDLQGNPVRRSIRGRWKACSFVVVYEVFERMAYYGISSNLFIYMTT 62

Query: 64  KLHQGTVTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLAVS 123
           KLHQGTV S+NNVTNW GT W+ PILGAY+ DA LGRY TF+I+ AI F+GM +LTL+V+
Sbjct: 63  KLHQGTVKSSNNVTNWVGTSWLTPILGAYVGDALLGRYITFVISCAIYFSGMMVLTLSVT 122

Query: 124 VPSLKPPACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFDPK 183
           +P +KPP C     +NC++AS LQLAVFFGALY LA+GTGGTKPNISTIGADQFD FDPK
Sbjct: 123 IPGIKPPECSTTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDVFDPK 182

Query: 184 EKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGTPF 243
           EK QKLSFFNWWMFSIFFGTLFA T+LVY+QDNVGW+LGYGLPT+GLAISI IF+ GTPF
Sbjct: 183 EKTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLLGTPF 242

Query: 244 YRHKLPNGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSLRF 303
           YRHKLP GSPFT+MA VI+A+    + P+ +D    HEL   EY ++GAF I  T SLRF
Sbjct: 243 YRHKLPTGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTPSLRF 302

Query: 304 LNKAAIIRRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLFIK 363
           L++A++         N +W L CT T+VEETKQMLRM+P+L  TF+PS MLAQ +TLF+K
Sbjct: 303 LDRASL-----KTGTNHKWNL-CTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVK 362

Query: 364 QGTTLDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQRMG 423
           QGTTLDR V   F IPPASL+ FVT+SML+S+V+YDR+FVKI +++T NPRGITLLQRMG
Sbjct: 363 QGTTLDRKVTGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMG 422

Query: 424 IGMILHIMIMTIASRVERHRLYIARENGLV-QNGGQIPLTIFSLLPQFMLMGTADAFIEV 483
           IG+I HI+IM +AS  ER+RL +A ++GL+ Q G ++PLTIF+LLPQF+LMG AD+F+EV
Sbjct: 423 IGLIFHILIMIVASVTERYRLKVAADHGLIHQTGVKLPLTIFALLPQFVLMGMADSFLEV 482

Query: 484 AKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGNGWILNNLNASH 543
           AK+EFFYDQAPE+MKSLGTSYS TSL IGNF+SSFLLSTVS IT + G GWILNNLN S 
Sbjct: 483 AKLEFFYDQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGRGWILNNLNESR 542

Query: 544 LDYYYALIAVLSAINFFIYLLVSKFYVYKAEVSGSI 579
           LDYYY   AVL+ +NF ++L+V KFYVY+AEV+ S+
Sbjct: 543 LDYYYLFFAVLNLVNFVLFLVVVKFYVYRAEVTDSV 571

BLAST of Tan0010424 vs. TAIR 10
Match: AT5G46040.1 (Major facilitator superfamily protein )

HSP 1 Score: 760.8 bits (1963), Expect = 8.3e-220
Identity = 384/575 (66.78%), Postives = 462/575 (80.35%), Query Frame = 0

Query: 4   VEETGLDDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYLTK 63
           VEE G DDYTKDGTVDL+GN V RS  GRWKACSF+VVYEVFERMAYYGIS+NL+IY+T 
Sbjct: 3   VEEVG-DDYTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYMTT 62

Query: 64  KLHQGTVTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLAVS 123
           KLHQGTV S+NNVTNW GT W+ PILGAY+ADAH GRY TF+I+SAI   GM LLTL+VS
Sbjct: 63  KLHQGTVKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLSVS 122

Query: 124 VPSLKPPACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFDPK 183
           +P LKPP C     +NC++AS +QLAVFFGALY LA+GTGGTKPNISTIGADQFDEFDPK
Sbjct: 123 LPGLKPPKCSTANVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFDPK 182

Query: 184 EKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGTPF 243
           +K  K SFFNWWMFSIFFGT FATT+LVY+QDNVGW++GYGL T+GLA SI IF+ GT  
Sbjct: 183 DKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGTRL 242

Query: 244 YRHKLPNGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSLRF 303
           YRHKLP GSPFT+MA VI+A+      P+ +D  + +EL   EY  + AF I STSSLRF
Sbjct: 243 YRHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTSSLRF 302

Query: 304 LNKAAIIRRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLFIK 363
           LN+A+ ++ G +     +W+L CT+T+VEETKQML+M+P+L  TF+PS MLAQ  TLFIK
Sbjct: 303 LNRAS-LKTGSTH----KWRL-CTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIK 362

Query: 364 QGTTLDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQRMG 423
           QGTTLDR +  +F IPPASL  F T SML+S+VIYDR+FVK M++ T NPRGITLLQRMG
Sbjct: 363 QGTTLDRRLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMG 422

Query: 424 IGMILHIMIMTIASRVERHRLYIARENGLV-QNGGQIPLTIFSLLPQFMLMGTADAFIEV 483
           IGMILHI+IM IAS  ER+RL +A E+GL  Q    IPL+IF+LLPQ++LMG ADAFIE+
Sbjct: 423 IGMILHILIMIIASITERYRLKVAAEHGLTHQTAVPIPLSIFTLLPQYVLMGLADAFIEI 482

Query: 484 AKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGNGWILNNLNASH 543
           AK+EFFYDQAPE+MKSLGTSY+ TS+ +G F+SS LLS+VS IT + G GWI NNLN S 
Sbjct: 483 AKLEFFYDQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGRGWIQNNLNESR 542

Query: 544 LDYYYALIAVLSAINFFIYLLVSKFYVYKAEVSGS 578
           LD YY   AVL+ +NF ++L+V +FY Y+A+V+ S
Sbjct: 543 LDNYYMFFAVLNLLNFILFLVVIRFYEYRADVTQS 570

BLAST of Tan0010424 vs. TAIR 10
Match: AT2G40460.1 (Major facilitator superfamily protein )

HSP 1 Score: 570.1 bits (1468), Expect = 2.1e-162
Identity = 289/564 (51.24%), Postives = 396/564 (70.21%), Query Frame = 0

Query: 12  YTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYLTKKLHQGTVT 71
           YT+DGTVDL+G PVL S  GRW+ACSF++ YE FERMA+YGI++NL+ YLTK+LH+ T++
Sbjct: 7   YTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDTIS 66

Query: 72  SANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLAVSVPSLKPPA 131
           S  NV NW+G VW+ PI GAYIAD+++GR+ TF  +S I   GM LLT+AV+V SL+P  
Sbjct: 67  SVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLRPTC 126

Query: 132 CLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFDPKEKAQKLSF 191
               +   C +AS LQ+  F+ +LY +A+G GGTKPNIST GADQFD +  +EK QK+SF
Sbjct: 127 ----ENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVSF 186

Query: 192 FNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGTPFYRHKLPNG 251
           FNWWMFS F G LFAT  LVYIQ+N+GW LGYG+PT+GL +S+++F  GTPFYRHK+   
Sbjct: 187 FNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIKT 246

Query: 252 SPFTR-MANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSLRFLNKAAII 311
               + +  V IAA  N  L  P+D  +L+ELD   Y   G  ++  T   RFL+KAAI 
Sbjct: 247 DNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAAI- 306

Query: 312 RRGDSRDHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLFIKQGTTLDR 371
                       ++ CTVT+VE  K++L +I I + T IPST+ AQ +TLF+KQGTTLDR
Sbjct: 307 --------KTSSRVPCTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDR 366

Query: 372 SVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQRMGIGMILHI 431
            +G++FQIP ASL +FVT+SMLLSV +YD+ FV  M++ T NPRGITLLQR+G+G  + I
Sbjct: 367 KIGSNFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQI 426

Query: 432 MIMTIASRVERHRLYIARENGLVQNGGQIPLTIFSLLPQFMLMGTADAFIEVAKIEFFYD 491
           + + IAS VE  R+ + +E  +      +P++IF LLPQ+ L+G  D F  +  +EFFYD
Sbjct: 427 VAIAIASAVEVKRMRVIKEFHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYD 486

Query: 492 QAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQ-NGNGWILNNLNASHLDYYYAL 551
           Q+PE M+SLGT++  + +G+GNFL+SFL++ +  IT++  G  WI NNLN S LDYYY  
Sbjct: 487 QSPEEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYGF 546

Query: 552 IAVLSAINFFIYLLVSKFYVYKAE 574
           + V+S +N  +++  +  YVYK++
Sbjct: 547 LVVISIVNMGLFVWAASKYVYKSD 557

BLAST of Tan0010424 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 499.2 bits (1284), Expect = 4.5e-141
Identity = 265/569 (46.57%), Postives = 378/569 (66.43%), Query Frame = 0

Query: 10  DDYTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYLTKKLHQGT 69
           D YT+DGTVD+  NP  +   G WKAC FI+  E  ER+AYYG+ TNL+ YL  +L+QG 
Sbjct: 5   DVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGN 64

Query: 70  VTSANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLAVSVPSLKP 129
            T+ANNVTNW+GT ++ P++GA+IADA+LGRY T      I  +GM LLTL+ SVP LKP
Sbjct: 65  ATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKP 124

Query: 130 PACLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFDPKEKAQKL 189
             C     D C   S  Q AVFF ALY++ALGTGG KP +S+ GADQFDE D  EK +K 
Sbjct: 125 GNC---NADTCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKS 184

Query: 190 SFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGTPFYRHKLP 249
           SFFNW+ FSI  G L A T+LV+IQ NVGW  G+G+PT+ + I++  F  G+ FYR + P
Sbjct: 185 SFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRP 244

Query: 250 NGSPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSLRFLNKAAI 309
            GSP TR+  VI+AA    S+ +P D   L E   DE   +G+ ++  T +L+F +KAA+
Sbjct: 245 GGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV 304

Query: 310 IRRGDSRDHND--EWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLFIKQGTT 369
             + DS    +   W+L C+VTQVEE K ++ ++P+     + +T+ +Q  T+F+ QG T
Sbjct: 305 ESQSDSIKDGEVNPWRL-CSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 364

Query: 370 LDRSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQRMGIGMI 429
           +D+ +G +F+IP ASL+ F T+S+L    +YD+  + + +++T+N RG T LQRMGIG++
Sbjct: 365 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 424

Query: 430 LHIMIMTIASRVERHRL-YIARENGLVQNGGQIPLTIFSLLPQFMLMGTADAFIEVAKIE 489
           + I  M  A  +E  RL Y+   N   Q   QI ++IF  +PQ++L+G A+ F  + ++E
Sbjct: 425 VSIFAMITAGVLEVVRLDYVKTHNAYDQK--QIHMSIFWQIPQYLLIGCAEVFTFIGQLE 484

Query: 490 FFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNGN-GWILNNLNASHLDY 549
           FFYDQAP+ M+SL ++ S+T++ +GN+LS+ L++ V  IT +NG  GWI +NLN  HLDY
Sbjct: 485 FFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDY 544

Query: 550 YYALIAVLSAINFFIYLLVSKFYVYKAEV 575
           ++ L+A LS +NF +YL +SK Y YK  V
Sbjct: 545 FFYLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Tan0010424 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 498.4 bits (1282), Expect = 7.7e-141
Identity = 250/563 (44.40%), Postives = 379/563 (67.32%), Query Frame = 0

Query: 12  YTKDGTVDLKGNPVLRSTRGRWKACSFIVVYEVFERMAYYGISTNLIIYLTKKLHQGTVT 71
           Y +DG+VD  GNP L+   G WKAC FI+  E  ER+AYYGI+ NLI YLT KLHQG V+
Sbjct: 24  YAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVS 83

Query: 72  SANNVTNWTGTVWMMPILGAYIADAHLGRYRTFMIASAICFTGMGLLTLAVSVPSLKPPA 131
           +A NVT W GT ++ P++GA +ADA+ GRY T    S I F GM  LTL+ SVP+LKP  
Sbjct: 84  AATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE 143

Query: 132 CLEVKKDNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDEFDPKEKAQKLSF 191
           C+    D C  A+  Q A+FFG LY++ALGTGG KP +S+ GADQFD+ D +E+ +K SF
Sbjct: 144 CI---GDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASF 203

Query: 192 FNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAGTPFYRHKLPNG 251
           FNW+ FSI  G L ++++LV+IQ+N GW LG+G+PT+ + ++I  F  GTP YR + P G
Sbjct: 204 FNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGG 263

Query: 252 SPFTRMANVIIAAACNWSLPLPNDPKQLHELDFDEYTKQGAFRIDSTSSLRFLNKAAIIR 311
           SP TR++ V++A+    S+ +P D   L+E         G+ +I+ T   ++L+KAA+I 
Sbjct: 264 SPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 323

Query: 312 RGDSR--DHNDEWKLICTVTQVEETKQMLRMIPILMCTFIPSTMLAQTHTLFIKQGTTLD 371
             +S+  D+++ W+L CTVTQVEE K ++RM PI     I S + AQ  T+F++QG  ++
Sbjct: 324 EEESKSGDYSNSWRL-CTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMN 383

Query: 372 RSVGTHFQIPPASLAAFVTISMLLSVVIYDRIFVKIMQRWTKNPRGITLLQRMGIGMILH 431
             +G+ FQ+PPA+L  F T S+++ V +YDR  V + +++T   +G T +QRMGIG+ + 
Sbjct: 384 CKIGS-FQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVS 443

Query: 432 IMIMTIASRVERHRLYIARENGLVQNGGQIPLTIFSLLPQFMLMGTADAFIEVAKIEFFY 491
           ++ M  A+ VE  RL++A + GLV++G  +P+++   +PQ+ ++G A+ F  + ++EFFY
Sbjct: 444 VLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFY 503

Query: 492 DQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITNQNG-NGWILNNLNASHLDYYYA 551
           DQ+P+ M+SL ++ ++ +  +GN+LSS +L+ V++ T +NG  GWI +NLN+ HLDY++ 
Sbjct: 504 DQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYFFW 563

Query: 552 LIAVLSAINFFIYLLVSKFYVYK 572
           L+A LS +N  +Y   +  Y  K
Sbjct: 564 LLAGLSLVNMAVYFFSAARYKQK 581