Tan0010409 (gene) Snake gourd v1

BLAST of Tan0010409 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 2.1e-103
Identity = 184/321 (57.32%), Postives = 238/321 (74.14%), Query Frame = 0

Query: 17  LPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVKPIHGVLSFDVIVDSSR 76
           LP KTR+++++ S   D A RP+GTINRR  R FD ++ PNP KP++ V + D +VD SR
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNP-KPVNIVSTSDFVVDQSR 72

Query: 77  NLSVRVFTPSSDAPSLPILIFFHGGGFAFLSNASFSYVAVCRRFARRIPAVVLSVDYRLS 136
           +L  R++TP      +P+++FFHGGGFAFLS  ++ Y  VCRRFAR++PA V+SV+YRL+
Sbjct: 73  DLWFRLYTPHVSGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNYRLA 132

Query: 137 PEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQ 196
           PEHR+P+QYDDGFD L++++   N   +LP  ADLS+CF AGDSAG N+AH+VA+R  R+
Sbjct: 133 PEHRYPAQYDDGFDALKYIE--ENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRICRE 192

Query: 197 -RSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTDWLWRAFLPEGSDRDHF 256
            RS F   K+IGL+SIQPFFGGEERTE+E +L    +VS  RTDW W+A    G +RDH 
Sbjct: 193 PRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRDHE 252

Query: 257 AANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLKESGTKVDLIEYPNMIHA 316
           A NV GPNA  IS L ++P T+V VAGFDPLKD QR YYEWLK  G K  LIEYPNM HA
Sbjct: 253 AVNVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMFHA 312

Query: 317 FYLFPELPESSVMINQVKEFV 337
           FY+FPELPE+  +I ++K+FV
Sbjct: 313 FYIFPELPEAGQLIMRIKDFV 326

BLAST of Tan0010409 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 5.3e-62
Identity = 132/334 (39.52%), Postives = 188/334 (56.29%), Query Frame = 0

Query: 17  LPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVKPIHGVLSFDVIVDSSR 76
           +P  T + +S   L  +L  RP+GT NR L  F D +  P    P++GV SFDVI+D   
Sbjct: 16  VPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLD-RKVPANANPVNGVFSFDVIIDRQT 75

Query: 77  NLSVRVFTPSS--------------DAPSLPILIFFHGGGFAFLSNASFSYVAVCRRFAR 136
           NL  RV+ P+               D   +P+++FFHGG FA  S  S  Y  +CRR   
Sbjct: 76  NLLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVG 135

Query: 137 RIPAVVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAGDSAG 196
              AVV+SV+YR +PE+R+P  YDDG+ VL+++   N++  L        + FLAGDS+G
Sbjct: 136 LCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWV---NSSSWLRSKKDSKVRIFLAGDSSG 195

Query: 197 ANLAHHVAVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTDWLW 256
            N+ H+VAVR    R       ++G + + P FGG ERTESE RLD  Y V++   DW W
Sbjct: 196 GNIVHNVAVRAVESR-----IDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYW 255

Query: 257 RAFLPEGSDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLKESGT 316
           RAFLPEG DR+H A +  GP ++ +  L  FP ++V VAG D ++D Q +Y E LK++G 
Sbjct: 256 RAFLPEGEDREHPACSPFGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQ 315

Query: 317 KVDLIEYPNMIHAFYLFPELPESSVMINQVKEFV 337
           +V L+        FYL P       +++++  FV
Sbjct: 316 EVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFV 339

BLAST of Tan0010409 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 6.8e-57
Identity = 129/337 (38.28%), Postives = 181/337 (53.71%), Query Frame = 0

Query: 17  LPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVKPIHGVLSFDVIVDSSR 76
           +P  T + +S   L   +  RP+G+ NR L  F D K   N   P+ GV SFD  VDS+ 
Sbjct: 16  VPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSF-PLDGVFSFD-HVDSTT 75

Query: 77  NLSVRVFTPS-----------------SDAPSLPILIFFHGGGFAFLSNASFSYVAVCRR 136
           NL  R++ P+                 S    +P+LIFFHGG F   S  S  Y   CRR
Sbjct: 76  NLLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRR 135

Query: 137 FARRIPAVVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAGD 196
                  VV+SVDYR SPEHR+P  YDDG++ L ++    + V L          +LAGD
Sbjct: 136 LVTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWV---KSRVWLQSGKDSNVYVYLAGD 195

Query: 197 SAGANLAHHVAVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTD 256
           S+G N+AH+VAVR +      +  K++G + + P FGG+ERT+SE  LD  Y V+I   D
Sbjct: 196 SSGGNIAHNVAVRATN-----EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRD 255

Query: 257 WLWRAFLPEGSDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLKE 316
           W WRA+LPEG DRDH A N  GP  + +  +  FP ++V VAG D ++D Q  Y + LK+
Sbjct: 256 WYWRAYLPEGEDRDHPACNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKK 315

Query: 317 SGTKVDLIEYPNMIHAFYLFPELPESSVMINQVKEFV 337
           +G +V+L+        FY  P       ++ ++ +FV
Sbjct: 316 TGLEVNLLYLKQATIGFYFLPNNDHFHCLMEELNKFV 341

BLAST of Tan0010409 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 3.7e-55
Identity = 124/344 (36.05%), Postives = 185/344 (53.78%), Query Frame = 0

Query: 17  LPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVKPIHGVLSFDVIVDSSR 76
           +P  T + +S   L  ++  R +GT  R L  + D +  P   +P+ GV SFD I+D S 
Sbjct: 16  VPLHTWVLISNFKLSYNILRRADGTFERDLGEYLD-RRVPANARPLEGVSSFDHIIDQSV 75

Query: 77  NLSVRVFTPS--------------------SDAPS---LPILIFFHGGGFAFLSNASFSY 136
            L VR++  +                    +DAP+    P++IFFHGG F   S +S  Y
Sbjct: 76  GLEVRIYRAAAEGDAEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIY 135

Query: 137 VAVCRRFARRIPAVVLSVDYRLSPEHRFPSQYDDGFDVLRF-LDHPNNTVGLLPPTADLS 196
            ++CRRF +    VV+SV+YR +PEHR+P  YDDG+  L++ +  P    G        +
Sbjct: 136 DSLCRRFVKLSKGVVVSVNYRRAPEHRYPCAYDDGWTALKWVMSQPFMRSG----GDAQA 195

Query: 197 KCFLAGDSAGANLAHHVAVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYI 256
           + FL+GDS+G N+AHHVAV     R+  +  K+ G + +   FGG ERTESE RLD  Y 
Sbjct: 196 RVFLSGDSSGGNIAHHVAV-----RAADEGVKVCGNILLNAMFGGTERTESERRLDGKYF 255

Query: 257 VSIARTDWLWRAFLPEGSDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRR 316
           V++   DW W+A+LPE +DRDH A N  GPN   +  L  F  +++ V+G D   DRQ  
Sbjct: 256 VTLQDRDWYWKAYLPEDADRDHPACNPFGPNGRRLGGL-PFAKSLIIVSGLDLTCDRQLA 315

Query: 317 YYEWLKESGTKVDLIEYPNMIHAFYLFPELPESSVMINQVKEFV 337
           Y + L+E G  V +++  N    FYL P       ++ ++ +F+
Sbjct: 316 YADALREDGHHVKVVQCENATVGFYLLPNTVHYHEVMEEISDFL 348

BLAST of Tan0010409 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 8.3e-55
Identity = 122/336 (36.31%), Postives = 177/336 (52.68%), Query Frame = 0

Query: 17  LPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVKPIHGVLSFDVIVDSSR 76
           +P  T + +S   +  ++  RP+GT NR L  + D K   N   P+ GV SFDV++D   
Sbjct: 16  VPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTAN-ANPVDGVFSFDVLIDRRI 75

Query: 77  NLSVRVFTPS----------------SDAPSLPILIFFHGGGFAFLSNASFSYVAVCRRF 136
           NL  RV+ P+                 D   +P+++FFHGG FA  S  S  Y  +CRR 
Sbjct: 76  NLLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRL 135

Query: 137 ARRIPAVVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAGDS 196
                 VV+SV+YR +PE+ +P  YDDG+  L ++   N+   L          FLAGDS
Sbjct: 136 VGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWV---NSRSWLKSKKDSKVHIFLAGDS 195

Query: 197 AGANLAHHVAVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTDW 256
           +G N+AH+VA+R            ++G + + P FGG ERTESE  LD  Y V++   DW
Sbjct: 196 SGGNIAHNVALRAGE-----SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDW 255

Query: 257 LWRAFLPEGSDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLKES 316
            W+AFLPEG DR+H A N   P  + +  +  FP ++V VAG D ++D Q  Y E LK++
Sbjct: 256 YWKAFLPEGEDREHPACNPFSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKA 315

Query: 317 GTKVDLIEYPNMIHAFYLFPELPESSVMINQVKEFV 337
           G +V L+        FYL P       +++++  FV
Sbjct: 316 GQEVKLMHLEKATVGFYLLPNNNHFHNVMDEISAFV 341

BLAST of Tan0010409 vs. NCBI nr
Match: XP_038896610.1 (probable carboxylesterase 18 [Benincasa hispida])

HSP 1 Score: 614.4 bits (1583), Expect = 6.1e-172
Identity = 296/335 (88.36%), Postives = 314/335 (93.73%), Query Frame = 0

Query: 8   PPAAAKSPALPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVKPIHGVLS 67
           PP  +KSP+LPW+TRL V ISS+CGDLATRPNGTINRRLFR FD KS  NPVKPIHGVLS
Sbjct: 62  PPPISKSPSLPWRTRLLVCISSICGDLATRPNGTINRRLFRLFDVKSPANPVKPIHGVLS 121

Query: 68  FDVIVDSSRNLSVRVFTPSSDAPSLPILIFFHGGGFAFLSNASFSYVAVCRRFARRIPAV 127
           FDVIVDSSRNLSVRVFTPSSDA SLP+LIFFHGGGFAFLSN+SFSY AVCRR ARRIPAV
Sbjct: 122 FDVIVDSSRNLSVRVFTPSSDAASLPVLIFFHGGGFAFLSNSSFSYAAVCRRLARRIPAV 181

Query: 128 VLSVDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAGDSAGANLAH 187
           VLSVDYRLSPEHRFPSQYDDGFDVLRFLDH NNTVGLLPP+ADLSKCFLAGDSAGANLAH
Sbjct: 182 VLSVDYRLSPEHRFPSQYDDGFDVLRFLDHENNTVGLLPPSADLSKCFLAGDSAGANLAH 241

Query: 188 HVAVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTDWLWRAFLP 247
           HVAVRF RQRSQFQ+AKIIGL+SIQPFFGGEERTESEIRLDPGYIVSIARTDWLW+AFLP
Sbjct: 242 HVAVRFCRQRSQFQRAKIIGLVSIQPFFGGEERTESEIRLDPGYIVSIARTDWLWKAFLP 301

Query: 248 EGSDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLKESGTKVDLI 307
           EG+DRDH AANVSGPNAE IS+LEEFPATVVFV GFDPLKD QRRYY+WLK+SG KVDLI
Sbjct: 302 EGADRDHAAANVSGPNAEEISELEEFPATVVFVGGFDPLKDWQRRYYDWLKKSGKKVDLI 361

Query: 308 EYPNMIHAFYLFPELPESSVMINQVKEFVLKCMEK 343
           E+PNMIHAFYLFPE+ ESSV+INQV+EFV KCM K
Sbjct: 362 EFPNMIHAFYLFPEICESSVLINQVREFVSKCMVK 396

BLAST of Tan0010409 vs. NCBI nr
Match: XP_022959604.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 610.1 bits (1572), Expect = 1.1e-170
Identity = 292/331 (88.22%), Postives = 308/331 (93.05%), Query Frame = 0

Query: 11  AAKSPALPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVKPIHGVLSFDV 70
           A KSP+LPWKTRL VS+SS+C DL TR NGT+NRRLFRFFD KSAPNPVKPIHGVLSFDV
Sbjct: 8   APKSPSLPWKTRLLVSLSSVCSDLVTRRNGTLNRRLFRFFDFKSAPNPVKPIHGVLSFDV 67

Query: 71  IVDSSRNLSVRVFTPSSDAPSLPILIFFHGGGFAFLSNASFSYVAVCRRFARRIPAVVLS 130
           IVDSSRNLSVRVFTP SDAPSLPIL+FFHGGGFAFLSNASFSY AVCRR ARRIPAVVLS
Sbjct: 68  IVDSSRNLSVRVFTPRSDAPSLPILVFFHGGGFAFLSNASFSYAAVCRRLARRIPAVVLS 127

Query: 131 VDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAGDSAGANLAHHVA 190
           VDYRLSPEHRFP QYDDGFDVLRFLDH NNT+GLLPP ADLSKCFLAGDSAGANLAHHVA
Sbjct: 128 VDYRLSPEHRFPCQYDDGFDVLRFLDHGNNTIGLLPPNADLSKCFLAGDSAGANLAHHVA 187

Query: 191 VRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTDWLWRAFLPEGS 250
           VRFSRQ+SQFQK KIIGL+SIQPFFGG ER ESE +LDPGYIVS+ RTDWLWRAFLPEGS
Sbjct: 188 VRFSRQKSQFQKVKIIGLISIQPFFGGVERMESETQLDPGYIVSLKRTDWLWRAFLPEGS 247

Query: 251 DRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLKESGTKVDLIEYP 310
           DRDH A+NVSGPNAE IS+LEEFPATVVFVAGFDPLKDRQRRY+EWLKESG KV+LIEYP
Sbjct: 248 DRDHSASNVSGPNAEAISELEEFPATVVFVAGFDPLKDRQRRYHEWLKESGKKVELIEYP 307

Query: 311 NMIHAFYLFPELPESSVMINQVKEFVLKCME 342
           NMIHAFYLFPELPESS MI+QV+ FV KCME
Sbjct: 308 NMIHAFYLFPELPESSAMIDQVRSFVFKCME 338

BLAST of Tan0010409 vs. NCBI nr
Match: KAG6593069.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 608.6 bits (1568), Expect = 3.3e-170
Identity = 292/338 (86.39%), Postives = 311/338 (92.01%), Query Frame = 0

Query: 4   ASHKPPAAAKSPALPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVKPIH 63
           AS    AA KSP+LPWKTRL VS+SS+C DL TR NGT+NRRLFRFFD KSAPNPVKPIH
Sbjct: 2   ASAAAAAAPKSPSLPWKTRLLVSLSSVCSDLVTRRNGTLNRRLFRFFDFKSAPNPVKPIH 61

Query: 64  GVLSFDVIVDSSRNLSVRVFTPSSDAPSLPILIFFHGGGFAFLSNASFSYVAVCRRFARR 123
           GVLSFDVIVDSSRNLSVRVFTP SDAPSLPIL+FFHGGGFAFLSNASFSY AVCRR ARR
Sbjct: 62  GVLSFDVIVDSSRNLSVRVFTPHSDAPSLPILVFFHGGGFAFLSNASFSYAAVCRRLARR 121

Query: 124 IPAVVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAGDSAGA 183
           IPAVVLSVDYRLSPEHRFP QYDDGFDVLRFLDH NNT+GLLPP ADLSKCFLAGDSAGA
Sbjct: 122 IPAVVLSVDYRLSPEHRFPCQYDDGFDVLRFLDHGNNTIGLLPPNADLSKCFLAGDSAGA 181

Query: 184 NLAHHVAVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTDWLWR 243
           NLAHHV+VRFSRQ+SQFQK KIIGL+SIQPFFGG ER ESE +LDPGYIVS+ RTDWLWR
Sbjct: 182 NLAHHVSVRFSRQKSQFQKVKIIGLISIQPFFGGVERMESETQLDPGYIVSLKRTDWLWR 241

Query: 244 AFLPEGSDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLKESGTK 303
           AFLPEGSDRDH A+NVSGPNAE I+++EEFPATVVFVAGFDPLKDRQRRY+EWLKESG K
Sbjct: 242 AFLPEGSDRDHSASNVSGPNAEAIAEVEEFPATVVFVAGFDPLKDRQRRYHEWLKESGKK 301

Query: 304 VDLIEYPNMIHAFYLFPELPESSVMINQVKEFVLKCME 342
           V+LIEYPNMIHAFYLFPELPESS MI+QV+ FV KCME
Sbjct: 302 VELIEYPNMIHAFYLFPELPESSAMIDQVRSFVFKCME 339

BLAST of Tan0010409 vs. NCBI nr
Match: XP_023514651.1 (probable carboxylesterase 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 607.8 bits (1566), Expect = 5.7e-170
Identity = 294/341 (86.22%), Postives = 311/341 (91.20%), Query Frame = 0

Query: 1   MDFASHKPPAAAKSPALPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVK 60
           M  A+  PP   KSP+LPWKTRL VS+SS+C DL TR NGT+NRRLFRFFD KSAPNPVK
Sbjct: 1   MASAAAAPP---KSPSLPWKTRLLVSLSSVCSDLVTRRNGTLNRRLFRFFDFKSAPNPVK 60

Query: 61  PIHGVLSFDVIVDSSRNLSVRVFTPSSDAPSLPILIFFHGGGFAFLSNASFSYVAVCRRF 120
           PIHGVLSFDVIVDSSRNLSVRVFTP SDAPSLPIL+FFHGGGFAFLSNASFSY AVCRR 
Sbjct: 61  PIHGVLSFDVIVDSSRNLSVRVFTPHSDAPSLPILVFFHGGGFAFLSNASFSYAAVCRRL 120

Query: 121 ARRIPAVVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAGDS 180
           ARRIPAVVLSVDYRLSPEHRFP QYDDGFDVLRFLDH NNT+GLLPP ADLSKCFLAGDS
Sbjct: 121 ARRIPAVVLSVDYRLSPEHRFPCQYDDGFDVLRFLDHGNNTIGLLPPNADLSKCFLAGDS 180

Query: 181 AGANLAHHVAVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTDW 240
           AGANLAHHVAVRFSRQ+SQFQK KIIGL+SIQPFFGG ER ESE +LDPGYIVS+ RTDW
Sbjct: 181 AGANLAHHVAVRFSRQKSQFQKVKIIGLISIQPFFGGVERMESETQLDPGYIVSLKRTDW 240

Query: 241 LWRAFLPEGSDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLKES 300
           LWRAFLPEGSDRDH A+NVSGPNAE IS+LEEFPATVVFVAGFDPLKDRQRRYYEWLK+S
Sbjct: 241 LWRAFLPEGSDRDHSASNVSGPNAEAISELEEFPATVVFVAGFDPLKDRQRRYYEWLKQS 300

Query: 301 GTKVDLIEYPNMIHAFYLFPELPESSVMINQVKEFVLKCME 342
           G KV+LIEY NMIHAFYLFPELPESS MI+QV+ FV KCME
Sbjct: 301 GKKVELIEYRNMIHAFYLFPELPESSAMIDQVRSFVFKCME 338

BLAST of Tan0010409 vs. NCBI nr
Match: KAA0059632.1 (putative carboxylesterase 18 [Cucumis melo var. makuwa])

HSP 1 Score: 607.8 bits (1566), Expect = 5.7e-170
Identity = 291/345 (84.35%), Postives = 317/345 (91.88%), Query Frame = 0

Query: 1   MDFAS---HKPPAAAKSPALPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPN 60
           MDF S     PP    SP+LPWKTRL VS+SS+CGDLATRPNGT+NRRLFR FD KS PN
Sbjct: 1   MDFPSPPPPPPPPPPSSPSLPWKTRLLVSLSSVCGDLATRPNGTVNRRLFRLFDFKSPPN 60

Query: 61  PVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDAPSLPILIFFHGGGFAFLSNASFSYVAVC 120
           PVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDA SLPILIFFHGGGFA LSN+SFSYVAVC
Sbjct: 61  PVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDAASLPILIFFHGGGFALLSNSSFSYVAVC 120

Query: 121 RRFARRIPAVVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLA 180
           RRFARR+PA+VLSVDYRLSPEHRFPSQYDDGFDVLRFLDH +NT+GLLPP ADLS CFLA
Sbjct: 121 RRFARRLPAIVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHESNTIGLLPPNADLSNCFLA 180

Query: 181 GDSAGANLAHHVAVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIAR 240
           GDSAGANLAHHVAVRF RQRSQF++A+++GL+SIQPFFGGEERTE+EI+LDPGYIVSIAR
Sbjct: 181 GDSAGANLAHHVAVRFCRQRSQFERARVVGLVSIQPFFGGEERTEAEIQLDPGYIVSIAR 240

Query: 241 TDWLWRAFLPEGSDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWL 300
           TDWLWRAFLPEG+DRDH AANVSG NAE IS+LEEFPATVVFV GFDPLKD QRRYY+WL
Sbjct: 241 TDWLWRAFLPEGADRDHGAANVSGENAEEISELEEFPATVVFVGGFDPLKDWQRRYYDWL 300

Query: 301 KESGTKVDLIEYPNMIHAFYLFPELPESSVMINQVKEFVLKCMEK 343
           K++G KVDLIEYPNMIHAFYLFPE+ ESSV++NQV+EFV KCMEK
Sbjct: 301 KKNGKKVDLIEYPNMIHAFYLFPEIGESSVLMNQVREFVSKCMEK 345

BLAST of Tan0010409 vs. ExPASy TrEMBL
Match: A0A6J1H8J6 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111460629 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.6e-171
Identity = 292/331 (88.22%), Postives = 308/331 (93.05%), Query Frame = 0

Query: 11  AAKSPALPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVKPIHGVLSFDV 70
           A KSP+LPWKTRL VS+SS+C DL TR NGT+NRRLFRFFD KSAPNPVKPIHGVLSFDV
Sbjct: 8   APKSPSLPWKTRLLVSLSSVCSDLVTRRNGTLNRRLFRFFDFKSAPNPVKPIHGVLSFDV 67

Query: 71  IVDSSRNLSVRVFTPSSDAPSLPILIFFHGGGFAFLSNASFSYVAVCRRFARRIPAVVLS 130
           IVDSSRNLSVRVFTP SDAPSLPIL+FFHGGGFAFLSNASFSY AVCRR ARRIPAVVLS
Sbjct: 68  IVDSSRNLSVRVFTPRSDAPSLPILVFFHGGGFAFLSNASFSYAAVCRRLARRIPAVVLS 127

Query: 131 VDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAGDSAGANLAHHVA 190
           VDYRLSPEHRFP QYDDGFDVLRFLDH NNT+GLLPP ADLSKCFLAGDSAGANLAHHVA
Sbjct: 128 VDYRLSPEHRFPCQYDDGFDVLRFLDHGNNTIGLLPPNADLSKCFLAGDSAGANLAHHVA 187

Query: 191 VRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTDWLWRAFLPEGS 250
           VRFSRQ+SQFQK KIIGL+SIQPFFGG ER ESE +LDPGYIVS+ RTDWLWRAFLPEGS
Sbjct: 188 VRFSRQKSQFQKVKIIGLISIQPFFGGVERMESETQLDPGYIVSLKRTDWLWRAFLPEGS 247

Query: 251 DRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLKESGTKVDLIEYP 310
           DRDH A+NVSGPNAE IS+LEEFPATVVFVAGFDPLKDRQRRY+EWLKESG KV+LIEYP
Sbjct: 248 DRDHSASNVSGPNAEAISELEEFPATVVFVAGFDPLKDRQRRYHEWLKESGKKVELIEYP 307

Query: 311 NMIHAFYLFPELPESSVMINQVKEFVLKCME 342
           NMIHAFYLFPELPESS MI+QV+ FV KCME
Sbjct: 308 NMIHAFYLFPELPESSAMIDQVRSFVFKCME 338

BLAST of Tan0010409 vs. ExPASy TrEMBL
Match: A0A5A7UUP4 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold54G00630 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 2.8e-170
Identity = 291/345 (84.35%), Postives = 317/345 (91.88%), Query Frame = 0

Query: 1   MDFAS---HKPPAAAKSPALPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPN 60
           MDF S     PP    SP+LPWKTRL VS+SS+CGDLATRPNGT+NRRLFR FD KS PN
Sbjct: 1   MDFPSPPPPPPPPPPSSPSLPWKTRLLVSLSSVCGDLATRPNGTVNRRLFRLFDFKSPPN 60

Query: 61  PVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDAPSLPILIFFHGGGFAFLSNASFSYVAVC 120
           PVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDA SLPILIFFHGGGFA LSN+SFSYVAVC
Sbjct: 61  PVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDAASLPILIFFHGGGFALLSNSSFSYVAVC 120

Query: 121 RRFARRIPAVVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLA 180
           RRFARR+PA+VLSVDYRLSPEHRFPSQYDDGFDVLRFLDH +NT+GLLPP ADLS CFLA
Sbjct: 121 RRFARRLPAIVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHESNTIGLLPPNADLSNCFLA 180

Query: 181 GDSAGANLAHHVAVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIAR 240
           GDSAGANLAHHVAVRF RQRSQF++A+++GL+SIQPFFGGEERTE+EI+LDPGYIVSIAR
Sbjct: 181 GDSAGANLAHHVAVRFCRQRSQFERARVVGLVSIQPFFGGEERTEAEIQLDPGYIVSIAR 240

Query: 241 TDWLWRAFLPEGSDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWL 300
           TDWLWRAFLPEG+DRDH AANVSG NAE IS+LEEFPATVVFV GFDPLKD QRRYY+WL
Sbjct: 241 TDWLWRAFLPEGADRDHGAANVSGENAEEISELEEFPATVVFVGGFDPLKDWQRRYYDWL 300

Query: 301 KESGTKVDLIEYPNMIHAFYLFPELPESSVMINQVKEFVLKCMEK 343
           K++G KVDLIEYPNMIHAFYLFPE+ ESSV++NQV+EFV KCMEK
Sbjct: 301 KKNGKKVDLIEYPNMIHAFYLFPEIGESSVLMNQVREFVSKCMEK 345

BLAST of Tan0010409 vs. ExPASy TrEMBL
Match: A0A6J1L0P0 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111498022 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 8.0e-170
Identity = 290/332 (87.35%), Postives = 308/332 (92.77%), Query Frame = 0

Query: 10  AAAKSPALPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVKPIHGVLSFD 69
           AA KSP+LPWKTRL VS+SS+C DL TR NGT+NRRLFR FD KS+PNPVKPIHGVLSFD
Sbjct: 6   AAPKSPSLPWKTRLLVSLSSVCSDLVTRRNGTLNRRLFRLFDFKSSPNPVKPIHGVLSFD 65

Query: 70  VIVDSSRNLSVRVFTPSSDAPSLPILIFFHGGGFAFLSNASFSYVAVCRRFARRIPAVVL 129
           VIVDSSRNLSVRVFTP SDAP+LPILIFFHGGGFAFLSNASFSY AVCRR ARRIPAVVL
Sbjct: 66  VIVDSSRNLSVRVFTPRSDAPTLPILIFFHGGGFAFLSNASFSYAAVCRRLARRIPAVVL 125

Query: 130 SVDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAGDSAGANLAHHV 189
           SVDYRLSPEHRFP QYDDGFDVLRFLDH NNT+GLLPP ADLSKCFLAGDSAGANLAHHV
Sbjct: 126 SVDYRLSPEHRFPCQYDDGFDVLRFLDHGNNTIGLLPPNADLSKCFLAGDSAGANLAHHV 185

Query: 190 AVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTDWLWRAFLPEG 249
           AVRFSRQ+SQFQK KIIGL+SIQPFFGG +R ESE +LDPGYIVS+ RTDWLWRAFLPEG
Sbjct: 186 AVRFSRQKSQFQKVKIIGLISIQPFFGGVKRMESETQLDPGYIVSLKRTDWLWRAFLPEG 245

Query: 250 SDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLKESGTKVDLIEY 309
           SDRDH A+NVSGPNAE I++LEEFPATVVFVAGFDPLKDRQRRYYEWLKESG KV+LIEY
Sbjct: 246 SDRDHSASNVSGPNAEAIAELEEFPATVVFVAGFDPLKDRQRRYYEWLKESGKKVELIEY 305

Query: 310 PNMIHAFYLFPELPESSVMINQVKEFVLKCME 342
           PNMIHAFYLFPELPESS MI+QV+ FV KCME
Sbjct: 306 PNMIHAFYLFPELPESSAMIDQVRSFVFKCME 337

BLAST of Tan0010409 vs. ExPASy TrEMBL
Match: A0A1S3BR05 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103492592 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 8.0e-170
Identity = 291/346 (84.10%), Postives = 316/346 (91.33%), Query Frame = 0

Query: 1   MDFAS----HKPPAAAKSPALPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAP 60
           MDF S      PP    SP+LPWKTRL VS+SS+CGDLATRPNGT+NRRLFR FD KS P
Sbjct: 1   MDFPSPPPPPPPPPPPSSPSLPWKTRLLVSLSSVCGDLATRPNGTVNRRLFRLFDFKSPP 60

Query: 61  NPVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDAPSLPILIFFHGGGFAFLSNASFSYVAV 120
           NPVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDA SLPILIFFHGGGFA LSN+SFSYVAV
Sbjct: 61  NPVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDAASLPILIFFHGGGFALLSNSSFSYVAV 120

Query: 121 CRRFARRIPAVVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFL 180
           CRRFARR+PA+VLSVDYRLSPEHRFPSQYDDGFDVLRFLDH +NT+GLLPP ADLS CFL
Sbjct: 121 CRRFARRLPAIVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHESNTIGLLPPNADLSNCFL 180

Query: 181 AGDSAGANLAHHVAVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIA 240
           AGDSAGANLAHHVAVRF RQRSQF++A+++GL+SIQPFFGGEERTE+EI+LDPGYIVSIA
Sbjct: 181 AGDSAGANLAHHVAVRFCRQRSQFERARVVGLVSIQPFFGGEERTEAEIQLDPGYIVSIA 240

Query: 241 RTDWLWRAFLPEGSDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEW 300
           RTDWLWRAFLPEG+DRDH AANVSG NAE IS+LEEFPATVVFV GFDPLKD QRRYY W
Sbjct: 241 RTDWLWRAFLPEGADRDHGAANVSGENAEEISELEEFPATVVFVGGFDPLKDWQRRYYAW 300

Query: 301 LKESGTKVDLIEYPNMIHAFYLFPELPESSVMINQVKEFVLKCMEK 343
           LK++G KVDLIEYPNMIHAFYLFPE+ ESSV++NQV+EFV KCMEK
Sbjct: 301 LKKNGKKVDLIEYPNMIHAFYLFPEIGESSVLMNQVREFVSKCMEK 346

BLAST of Tan0010409 vs. ExPASy TrEMBL
Match: A0A0A0KA95 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375730 PE=3 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.5e-168
Identity = 287/344 (83.43%), Postives = 316/344 (91.86%), Query Frame = 0

Query: 1   MDFAS--HKPPAAAKSPALPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNP 60
           MDF S    PP    SP+LPWKTRL VS+SS+CGDLATRPNGT+NRRLFR FD KS PNP
Sbjct: 1   MDFPSPPPPPPPPPSSPSLPWKTRLLVSLSSVCGDLATRPNGTVNRRLFRLFDFKSPPNP 60

Query: 61  VKPIHGVLSFDVIVDSSRNLSVRVFTPSSDAPSLPILIFFHGGGFAFLSNASFSYVAVCR 120
           VKPIHGVLSFDVIVDSSRNLSVRVFTPSSD  SLPILIFFHGGGFA LSN+SFSYVAVCR
Sbjct: 61  VKPIHGVLSFDVIVDSSRNLSVRVFTPSSDVASLPILIFFHGGGFALLSNSSFSYVAVCR 120

Query: 121 RFARRIPAVVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAG 180
           RFARR+PA+VLSVDYRLSPEHRFPSQYDDGFDVLRFLDH +NT+GLLPP ADLSKCFLAG
Sbjct: 121 RFARRLPAIVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHESNTIGLLPPNADLSKCFLAG 180

Query: 181 DSAGANLAHHVAVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIART 240
           DSAGANLAHHVAVRF RQRSQF++A+++GL+SIQPFFGGEERTE+EI+LDPGYIVSIART
Sbjct: 181 DSAGANLAHHVAVRFCRQRSQFERARVVGLVSIQPFFGGEERTEAEIQLDPGYIVSIART 240

Query: 241 DWLWRAFLPEGSDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLK 300
           DWLWRAFLPEG+DRDH AANVSG NAE IS+LEEFPAT+VFV GFDPLKD QRRYY+WLK
Sbjct: 241 DWLWRAFLPEGADRDHGAANVSGENAEEISELEEFPATLVFVGGFDPLKDWQRRYYDWLK 300

Query: 301 ESGTKVDLIEYPNMIHAFYLFPELPESSVMINQVKEFVLKCMEK 343
           ++G  V+LIEYPNMIHAFYLFPE+ ESSV++N+V+EFV KCMEK
Sbjct: 301 KNGKIVELIEYPNMIHAFYLFPEISESSVLMNEVREFVSKCMEK 344

BLAST of Tan0010409 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 377.1 bits (967), Expect = 1.5e-104
Identity = 184/321 (57.32%), Postives = 238/321 (74.14%), Query Frame = 0

Query: 17  LPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVKPIHGVLSFDVIVDSSR 76
           LP KTR+++++ S   D A RP+GTINRR  R FD ++ PNP KP++ V + D +VD SR
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNP-KPVNIVSTSDFVVDQSR 72

Query: 77  NLSVRVFTPSSDAPSLPILIFFHGGGFAFLSNASFSYVAVCRRFARRIPAVVLSVDYRLS 136
           +L  R++TP      +P+++FFHGGGFAFLS  ++ Y  VCRRFAR++PA V+SV+YRL+
Sbjct: 73  DLWFRLYTPHVSGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNYRLA 132

Query: 137 PEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQ 196
           PEHR+P+QYDDGFD L++++   N   +LP  ADLS+CF AGDSAG N+AH+VA+R  R+
Sbjct: 133 PEHRYPAQYDDGFDALKYIE--ENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRICRE 192

Query: 197 -RSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTDWLWRAFLPEGSDRDHF 256
            RS F   K+IGL+SIQPFFGGEERTE+E +L    +VS  RTDW W+A    G +RDH 
Sbjct: 193 PRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRDHE 252

Query: 257 AANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLKESGTKVDLIEYPNMIHA 316
           A NV GPNA  IS L ++P T+V VAGFDPLKD QR YYEWLK  G K  LIEYPNM HA
Sbjct: 253 AVNVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMFHA 312

Query: 317 FYLFPELPESSVMINQVKEFV 337
           FY+FPELPE+  +I ++K+FV
Sbjct: 313 FYIFPELPEAGQLIMRIKDFV 326

BLAST of Tan0010409 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 239.6 bits (610), Expect = 3.8e-63
Identity = 132/334 (39.52%), Postives = 188/334 (56.29%), Query Frame = 0

Query: 17  LPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVKPIHGVLSFDVIVDSSR 76
           +P  T + +S   L  +L  RP+GT NR L  F D +  P    P++GV SFDVI+D   
Sbjct: 16  VPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLD-RKVPANANPVNGVFSFDVIIDRQT 75

Query: 77  NLSVRVFTPSS--------------DAPSLPILIFFHGGGFAFLSNASFSYVAVCRRFAR 136
           NL  RV+ P+               D   +P+++FFHGG FA  S  S  Y  +CRR   
Sbjct: 76  NLLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVG 135

Query: 137 RIPAVVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAGDSAG 196
              AVV+SV+YR +PE+R+P  YDDG+ VL+++   N++  L        + FLAGDS+G
Sbjct: 136 LCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWV---NSSSWLRSKKDSKVRIFLAGDSSG 195

Query: 197 ANLAHHVAVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTDWLW 256
            N+ H+VAVR    R       ++G + + P FGG ERTESE RLD  Y V++   DW W
Sbjct: 196 GNIVHNVAVRAVESR-----IDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYW 255

Query: 257 RAFLPEGSDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLKESGT 316
           RAFLPEG DR+H A +  GP ++ +  L  FP ++V VAG D ++D Q +Y E LK++G 
Sbjct: 256 RAFLPEGEDREHPACSPFGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQ 315

Query: 317 KVDLIEYPNMIHAFYLFPELPESSVMINQVKEFV 337
           +V L+        FYL P       +++++  FV
Sbjct: 316 EVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFV 339

BLAST of Tan0010409 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 222.6 bits (566), Expect = 4.8e-58
Identity = 129/337 (38.28%), Postives = 181/337 (53.71%), Query Frame = 0

Query: 17  LPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVKPIHGVLSFDVIVDSSR 76
           +P  T + +S   L   +  RP+G+ NR L  F D K   N   P+ GV SFD  VDS+ 
Sbjct: 16  VPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSF-PLDGVFSFD-HVDSTT 75

Query: 77  NLSVRVFTPS-----------------SDAPSLPILIFFHGGGFAFLSNASFSYVAVCRR 136
           NL  R++ P+                 S    +P+LIFFHGG F   S  S  Y   CRR
Sbjct: 76  NLLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRR 135

Query: 137 FARRIPAVVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAGD 196
                  VV+SVDYR SPEHR+P  YDDG++ L ++    + V L          +LAGD
Sbjct: 136 LVTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWV---KSRVWLQSGKDSNVYVYLAGD 195

Query: 197 SAGANLAHHVAVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTD 256
           S+G N+AH+VAVR +      +  K++G + + P FGG+ERT+SE  LD  Y V+I   D
Sbjct: 196 SSGGNIAHNVAVRATN-----EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRD 255

Query: 257 WLWRAFLPEGSDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLKE 316
           W WRA+LPEG DRDH A N  GP  + +  +  FP ++V VAG D ++D Q  Y + LK+
Sbjct: 256 WYWRAYLPEGEDRDHPACNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKK 315

Query: 317 SGTKVDLIEYPNMIHAFYLFPELPESSVMINQVKEFV 337
           +G +V+L+        FY  P       ++ ++ +FV
Sbjct: 316 TGLEVNLLYLKQATIGFYFLPNNDHFHCLMEELNKFV 341

BLAST of Tan0010409 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 215.7 bits (548), Expect = 5.9e-56
Identity = 122/336 (36.31%), Postives = 177/336 (52.68%), Query Frame = 0

Query: 17  LPWKTRLSVSISSLCGDLATRPNGTINRRLFRFFDCKSAPNPVKPIHGVLSFDVIVDSSR 76
           +P  T + +S   +  ++  RP+GT NR L  + D K   N   P+ GV SFDV++D   
Sbjct: 16  VPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTAN-ANPVDGVFSFDVLIDRRI 75

Query: 77  NLSVRVFTPS----------------SDAPSLPILIFFHGGGFAFLSNASFSYVAVCRRF 136
           NL  RV+ P+                 D   +P+++FFHGG FA  S  S  Y  +CRR 
Sbjct: 76  NLLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRL 135

Query: 137 ARRIPAVVLSVDYRLSPEHRFPSQYDDGFDVLRFLDHPNNTVGLLPPTADLSKCFLAGDS 196
                 VV+SV+YR +PE+ +P  YDDG+  L ++   N+   L          FLAGDS
Sbjct: 136 VGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWV---NSRSWLKSKKDSKVHIFLAGDS 195

Query: 197 AGANLAHHVAVRFSRQRSQFQKAKIIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTDW 256
           +G N+AH+VA+R            ++G + + P FGG ERTESE  LD  Y V++   DW
Sbjct: 196 SGGNIAHNVALRAGE-----SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDW 255

Query: 257 LWRAFLPEGSDRDHFAANVSGPNAEVISDLEEFPATVVFVAGFDPLKDRQRRYYEWLKES 316
            W+AFLPEG DR+H A N   P  + +  +  FP ++V VAG D ++D Q  Y E LK++
Sbjct: 256 YWKAFLPEGEDREHPACNPFSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKA 315

Query: 317 GTKVDLIEYPNMIHAFYLFPELPESSVMINQVKEFV 337
           G +V L+        FYL P       +++++  FV
Sbjct: 316 GQEVKLMHLEKATVGFYLLPNNNHFHNVMDEISAFV 341

BLAST of Tan0010409 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 174.9 bits (442), Expect = 1.1e-43
Identity = 108/314 (34.39%), Postives = 162/314 (51.59%), Query Frame = 0

Query: 32  GDLATRPNGTINRRLFRFFDCKSAPNPVKPIHGVLSFDVIVDSSRNLSVRVFTP--SSDA 91
           G L    NGT+ R      D  +   P K    VL  D I     NL +R++ P  +S+ 
Sbjct: 16  GLLQLLSNGTVLRS--ESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISASNR 75

Query: 92  PSLPILIFFHGGGFAFLSNASFSYVAVCRRFARRIPAVVLSVDYRLSPEHRFPSQYDDGF 151
            +LP+++FFHGGGF F S +   +   C   A  + A+V+S DYRL+PEHR P+ ++D  
Sbjct: 76  TALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFEDAE 135

Query: 152 DVLRFLDHPNNTVGLL-----PPTADLSKCFLAGDSAGANLAHHVAVRFSRQRSQFQKAK 211
            VL +L     + G+          D  + F+ GDS+G N+AH +AVRF     +    +
Sbjct: 136 AVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGSGSIELTPVR 195

Query: 212 IIGLLSIQPFFGGEERTESEIRLDPGYIVSIARTDWLWRAFLPEGSDRDHFAANVSGPNA 271
           + G + + PFFGGEERT SE       ++S+   D  WR  LP G+ RDH  AN  GP +
Sbjct: 196 VRGYVLMGPFFGGEERTNSENGPSEA-LLSLDLLDKFWRLSLPNGATRDHHMANPFGPTS 255

Query: 272 EVISDLEEFPATVVFVAGFDPLKDRQRRY-YEWLKESGTKVDLIEYPNMIHAFYL-FPEL 331
             +  +   P  V+ V G + L+DR + Y Y+  K  G +VD IE+ N  H FY  +P  
Sbjct: 256 PTLESISLEPMLVI-VGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGFYSNYPSS 315

Query: 332 PESSVMINQVKEFV 337
             +  ++  + +F+
Sbjct: 316 EAAEQVLRIIGDFM 325