Tan0010405 (gene) Snake gourd v1

Overview
NameTan0010405
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionMannan endo-1,4-beta-mannosidase
LocationLG04: 85094245 .. 85096408 (+)
RNA-Seq ExpressionTan0010405
SyntenyTan0010405
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTAAAACATTATTCCCTCTAATTGCTTTAATCCTCTTCTCTTTATTGGCTCTCTCTCAATCCTTACCTCTCTCCACAAGAGGAAGATGGATCGTAGATGCTCGAACTGGGCAACGAGTGAAGCTAACTTGTGTCAATTTGGTGTCTCATGCACAGTCCATGGTGGCTCAAGGGCTCGACAAGCGTCCATTGAAAGAGTTAGCCGCCGAGATCAAAAGTCGTTTCAACTGTGTTCGATTGACATGGTCCATTCACATGTTCACTCGTTACACATTTGAGACCATTGGAGACGTCTTTGACAATCTAGACCTTGCAAGTGTCAAGAGTGGAGTTCAAAAACACAACCCTCAAATATTAAACATGACGGTTGCCTATGCATTCAAAACCGTCATTGAGGGCTTAGGCTCAGAAGGGATCATGGTTATTCTAGATAACCACATTACCCAACCGAGATGGTGTTGCTCTTACAATGATGGAAACGGCTTCTTTGGAGACCGCAATTTCAACCCTCAAGAATGGCTCAAGGGCTTGTCCTATGTTGCTTCACACTTCACTTGGAATCCCCATGTAAGTTTAATTAAAAATGTCTTGTTTTAGAATTTCCTTTCCGTTGCATGCTAGATGGGTGCAGCTTTTGTCGTGTAATATTAATAATTGTGTGTGTGATGTGTAAAAGGTGATAGGAATGAGTCTAAGAAACGAGCTTCGAGGATCATTGACAAACAAAGACGTGTGGTACAAGTATATGGGAGAAGGAAGCCATTTGGTGCATAAAATAAACCCACATCTATTGGTAATAATTTCTGGATTAAATTACGACAATGATCTAAGCCATATAAAGAAGAAGCCATTAGGGTACAACTTAAACAACAAAGTAGTATTGGAAGCACATCTATACTCGTTCAGTGGAGAGAATGAAAAAAAATTTGTGAATAGACCATTGAACATAGTATGCGACCAAATAATGGATAAGTTCGAAAGAGAAGCTGGGTTTGTGGTGGACATGGAGAAACCATATCCTTTGTTTCTTAGTGAATTTGGGTACGATCAAAGAGGTGGCAATAAGGCTCAGAATCGCTTCATGAGTTGCTTTTTGGCAAGAATTGCAGCTAAGGATATTGATTGGGCCTTGTGGGCTTTCCAAGGAAGTTATATGTATAGACAAGTTCATCAAGATCTTGATGAAAGTTTTGGTGTTATGGATCATTCTTGGACTAAAGATCGTAACCCTAAACTCAAACAAATGCTTCAACTTCCCAAGAGATTGAATCAAGGTAATTTCTTTCATTTCTTCTTTTTAATGAGCATAATAGTGATCTATTGTTTGAATCCCCGACTTCAATTGTACTAAAAAAATTAAACCCATCTTTCTGCAATCCACTTATTTGTCCATTCACAATTTCTATACAGGTGTGGAACTCACACTTAAATATTTTTATTGACAAAAAAATAACATTAGAATTGCTTTTATATCAAGTTATTATTCTTAGCCTATGTGATAAATAGTCCATCTTTTATTTGTTCTAATCATACTATATTCTCAATATTCTTTTAGATCCGTCAACTGCTCAACTGGCCTCTTGGTTCATGACCAAGAGGTCATGGATTCGAATTCCTCATCCTCAATTGTTTTAAAAAAACCTAATTGAATAGCATATTCTCAATGCTCTTATTAAACATTTTAGTATATATATTTTATTAGAATTTGAAACATAAAATTTTTTTTTTTTTTTTTGCAGATCCAAATTCAAAAGCACCAATGTCCTACATAATGCTACATCCAGTGACAGGGCAATGCGTCAAATCCGACGGCAAAGGAGGAATTGAACTCGGAGACTGTACGGCACCGACCCAGTGGAGCCACGGCGGAGATGCAAGTCCGGTGAAGTTGCAGTCAAACGGACAGTGTTTGAAATCCGCCGGAGATGGAAAACCGCCGGTGGTATCCGCCGACTGCTCCGGCGACGGAAGCAACTGGACGGTGGCTTCGAAAGCAAAGCTCCAATTGGCAACAAAAATTGGCGGAGACCAATTTTGCTTAGAGAAGGAATCTGATACTTCAATCGTAATGAAGAAATGCATTTGCCTTACAAATGAGTGGAATTGCTTCGACGATCCTCAGAGCCAGTGGTTCAAGCTTGTTCCAACAAATGTTGCTTAA

mRNA sequence

ATGTCTAAAACATTATTCCCTCTAATTGCTTTAATCCTCTTCTCTTTATTGGCTCTCTCTCAATCCTTACCTCTCTCCACAAGAGGAAGATGGATCGTAGATGCTCGAACTGGGCAACGAGTGAAGCTAACTTGTGTCAATTTGGTGTCTCATGCACAGTCCATGGTGGCTCAAGGGCTCGACAAGCGTCCATTGAAAGAGTTAGCCGCCGAGATCAAAAGTCGTTTCAACTGTGTTCGATTGACATGGTCCATTCACATGTTCACTCGTTACACATTTGAGACCATTGGAGACGTCTTTGACAATCTAGACCTTGCAAGTGTCAAGAGTGGAGTTCAAAAACACAACCCTCAAATATTAAACATGACGGTTGCCTATGCATTCAAAACCGTCATTGAGGGCTTAGGCTCAGAAGGGATCATGGTTATTCTAGATAACCACATTACCCAACCGAGATGGTGTTGCTCTTACAATGATGGAAACGGCTTCTTTGGAGACCGCAATTTCAACCCTCAAGAATGGCTCAAGGGCTTGTCCTATGTTGCTTCACACTTCACTTGGAATCCCCATGTGATAGGAATGAGTCTAAGAAACGAGCTTCGAGGATCATTGACAAACAAAGACGTGTGGTACAAGTATATGGGAGAAGGAAGCCATTTGGTGCATAAAATAAACCCACATCTATTGGTAATAATTTCTGGATTAAATTACGACAATGATCTAAGCCATATAAAGAAGAAGCCATTAGGGTACAACTTAAACAACAAAGTAGTATTGGAAGCACATCTATACTCGTTCAGTGGAGAGAATGAAAAAAAATTTGTGAATAGACCATTGAACATAGTATGCGACCAAATAATGGATAAGTTCGAAAGAGAAGCTGGGTTTGTGGTGGACATGGAGAAACCATATCCTTTGTTTCTTAGTGAATTTGGGTACGATCAAAGAGGTGGCAATAAGGCTCAGAATCGCTTCATGAGTTGCTTTTTGGCAAGAATTGCAGCTAAGGATATTGATTGGGCCTTGTGGGCTTTCCAAGGAAGTTATATGTATAGACAAGTTCATCAAGATCTTGATGAAAGTTTTGGTGTTATGGATCATTCTTGGACTAAAGATCGTAACCCTAAACTCAAACAAATGCTTCAACTTCCCAAGAGATTGAATCAAGATCCAAATTCAAAAGCACCAATGTCCTACATAATGCTACATCCAGTGACAGGGCAATGCGTCAAATCCGACGGCAAAGGAGGAATTGAACTCGGAGACTGTACGGCACCGACCCAGTGGAGCCACGGCGGAGATGCAAGTCCGGTGAAGTTGCAGTCAAACGGACAGTGTTTGAAATCCGCCGGAGATGGAAAACCGCCGGTGGTATCCGCCGACTGCTCCGGCGACGGAAGCAACTGGACGGTGGCTTCGAAAGCAAAGCTCCAATTGGCAACAAAAATTGGCGGAGACCAATTTTGCTTAGAGAAGGAATCTGATACTTCAATCGTAATGAAGAAATGCATTTGCCTTACAAATGAGTGGAATTGCTTCGACGATCCTCAGAGCCAGTGGTTCAAGCTTGTTCCAACAAATGTTGCTTAA

Coding sequence (CDS)

ATGTCTAAAACATTATTCCCTCTAATTGCTTTAATCCTCTTCTCTTTATTGGCTCTCTCTCAATCCTTACCTCTCTCCACAAGAGGAAGATGGATCGTAGATGCTCGAACTGGGCAACGAGTGAAGCTAACTTGTGTCAATTTGGTGTCTCATGCACAGTCCATGGTGGCTCAAGGGCTCGACAAGCGTCCATTGAAAGAGTTAGCCGCCGAGATCAAAAGTCGTTTCAACTGTGTTCGATTGACATGGTCCATTCACATGTTCACTCGTTACACATTTGAGACCATTGGAGACGTCTTTGACAATCTAGACCTTGCAAGTGTCAAGAGTGGAGTTCAAAAACACAACCCTCAAATATTAAACATGACGGTTGCCTATGCATTCAAAACCGTCATTGAGGGCTTAGGCTCAGAAGGGATCATGGTTATTCTAGATAACCACATTACCCAACCGAGATGGTGTTGCTCTTACAATGATGGAAACGGCTTCTTTGGAGACCGCAATTTCAACCCTCAAGAATGGCTCAAGGGCTTGTCCTATGTTGCTTCACACTTCACTTGGAATCCCCATGTGATAGGAATGAGTCTAAGAAACGAGCTTCGAGGATCATTGACAAACAAAGACGTGTGGTACAAGTATATGGGAGAAGGAAGCCATTTGGTGCATAAAATAAACCCACATCTATTGGTAATAATTTCTGGATTAAATTACGACAATGATCTAAGCCATATAAAGAAGAAGCCATTAGGGTACAACTTAAACAACAAAGTAGTATTGGAAGCACATCTATACTCGTTCAGTGGAGAGAATGAAAAAAAATTTGTGAATAGACCATTGAACATAGTATGCGACCAAATAATGGATAAGTTCGAAAGAGAAGCTGGGTTTGTGGTGGACATGGAGAAACCATATCCTTTGTTTCTTAGTGAATTTGGGTACGATCAAAGAGGTGGCAATAAGGCTCAGAATCGCTTCATGAGTTGCTTTTTGGCAAGAATTGCAGCTAAGGATATTGATTGGGCCTTGTGGGCTTTCCAAGGAAGTTATATGTATAGACAAGTTCATCAAGATCTTGATGAAAGTTTTGGTGTTATGGATCATTCTTGGACTAAAGATCGTAACCCTAAACTCAAACAAATGCTTCAACTTCCCAAGAGATTGAATCAAGATCCAAATTCAAAAGCACCAATGTCCTACATAATGCTACATCCAGTGACAGGGCAATGCGTCAAATCCGACGGCAAAGGAGGAATTGAACTCGGAGACTGTACGGCACCGACCCAGTGGAGCCACGGCGGAGATGCAAGTCCGGTGAAGTTGCAGTCAAACGGACAGTGTTTGAAATCCGCCGGAGATGGAAAACCGCCGGTGGTATCCGCCGACTGCTCCGGCGACGGAAGCAACTGGACGGTGGCTTCGAAAGCAAAGCTCCAATTGGCAACAAAAATTGGCGGAGACCAATTTTGCTTAGAGAAGGAATCTGATACTTCAATCGTAATGAAGAAATGCATTTGCCTTACAAATGAGTGGAATTGCTTCGACGATCCTCAGAGCCAGTGGTTCAAGCTTGTTCCAACAAATGTTGCTTAA

Protein sequence

MSKTLFPLIALILFSLLALSQSLPLSTRGRWIVDARTGQRVKLTCVNLVSHAQSMVAQGLDKRPLKELAAEIKSRFNCVRLTWSIHMFTRYTFETIGDVFDNLDLASVKSGVQKHNPQILNMTVAYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLSYVASHFTWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLNYDNDLSHIKKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFEREAGFVVDMEKPYPLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQVHQDLDESFGVMDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCVKSDGKGGIELGDCTAPTQWSHGGDASPVKLQSNGQCLKSAGDGKPPVVSADCSGDGSNWTVASKAKLQLATKIGGDQFCLEKESDTSIVMKKCICLTNEWNCFDDPQSQWFKLVPTNVA
Homology
BLAST of Tan0010405 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 8.3e-115
Identity = 219/539 (40.63%), Postives = 318/539 (59.00%), Query Frame = 0

Query: 8   LIALILFSLLALSQSLPLSTRGRWIVDARTGQRVKLTCVNLVSHAQSMVAQGLDKRPLKE 67
           L AL+L  + A S SLPL TRGRWIVD  TG RVKL CVN V H +  + +GL++ P+  
Sbjct: 14  LTALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVAT 73

Query: 68  LAAEIKS-RFNCVRLTWSIHMFTR--YTFETIGDVFDNLDLASVKSGVQKHNPQILNMTV 127
           +A  I S  FNCVRLT+SIHM TR  YT  T+   F  L+L    SG++ +NP++L++  
Sbjct: 74  VAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLGH 133

Query: 128 AYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLSYVASH 187
             A+  V+  L   G+MVILDNH+++P+WCC+ +DGNGFFGDR FNP  W++GL  +A++
Sbjct: 134 VAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMATY 193

Query: 188 FTWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLNYDNDLSHI 247
           F   P+V+ MSLRNELRG+ +    W ++M  G+  VHK NP +LVI+SGL +D DLS +
Sbjct: 194 FNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFL 253

Query: 248 KKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFEREAGFVVDMEK-- 307
              P+      K+V E H YSF         N     VC     +F+   GFV       
Sbjct: 254 PVLPVTLPFKEKIVYEGHWYSFGVPWRTGLPND----VCKNETGRFKSNVGFVTSSANAT 313

Query: 308 PYPLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYR---QVHQDLD 367
             PLF+SEFG DQR  N   NR+++C LA +A +D+DWALW   GSY YR   Q  +D +
Sbjct: 314 AAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKDFE 373

Query: 368 ESFGVMDHSWTKDRNPKLKQMLQLPKRLNQDPN-SKAPMSYIMLHPVTGQCVKSDGKGGI 427
           E++G  +H W++ RNP     L+  ++  QDP  +  P   I+ HP +G CV+S     +
Sbjct: 374 ETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVESGIGNTV 433

Query: 428 ELGDC-TAPTQWSHGGDA-SPVKLQSNGQCLKSAGDGKPPVVSADCSGDGSN-WTVASKA 487
            LG C +  ++W++      P+ L  +  C+ + G+G P +++ +CS   +  W+  S A
Sbjct: 434 HLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVSSA 493

Query: 488 KLQLATKIGGDQ-----FCLEKESDTSIVMKKCICLTNEWNCFD--DPQSQWFKLVPTN 528
           +LQL T++ G        CL+      I   +CIC+T+  +C+   +P+ QWFK++ TN
Sbjct: 494 QLQLGTRVLGKDGKEKWMCLDGSKSPLISTNECICITDS-HCYPKLNPEKQWFKVITTN 547

BLAST of Tan0010405 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 2.8e-09
Identity = 96/400 (24.00%), Postives = 157/400 (39.25%), Query Frame = 0

Query: 2   SKTLFPLIALILFSLLALSQSLPLSTRGRWIVDARTGQRVKLTCVNLVS-HAQSMVAQGL 61
           + TL    AL L +  A S S+      R IVD  +G+ V+L  VN+      + V  GL
Sbjct: 7   ASTLALATALALAAGPAFSYSI---NNSRQIVD-DSGKVVQLKGVNVFGFETGNHVMHGL 66

Query: 62  DKRPLKELAAEIKS-RFNCVRLTWSIHMFTRYTFETIGDVFDNLDLASVKSGVQKHNPQI 121
             R  K++  +++   FN VRL +        T     D   N DL  + S       QI
Sbjct: 67  WARNWKDMIVQMQGLGFNAVRLPFCPATLRSDTMPASIDYSRNADLQGLTS------LQI 126

Query: 122 LNMTVAYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLS 181
           L+         VI    + G+ V+LD+H      C   ++    +   ++   +WL  L 
Sbjct: 127 LD--------KVIAEFNARGMYVLLDHHTPD---CAGISE---LWYTGSYTEAQWLADLR 186

Query: 182 YVASHFTWNPHVIGMSLRNELRGSLT-----NKDVWYKYMGEGSHLVHKINPHLLVIISG 241
           +VA+ +   P+V+G+ L+NE  G+ T         W K    GS  V  + P  L+ + G
Sbjct: 187 FVANRYKNVPYVLGLDLKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAPKWLIAVEG 246

Query: 242 LN------------YDNDLSHIKKKPLGYNLNNKVVLEAHLYS-----FSGENEKKFVNR 301
           +             +  +L  +   PL     N+++L  H+Y       S  N+  F N 
Sbjct: 247 ITDNPVCSTNGGIFWGGNLQPLACTPLNIPA-NRLLLAPHVYGPDVFVQSYFNDSNFPNN 306

Query: 302 PLNIVCDQIMDKFEREAGFVVDMEKPYPLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKD 361
              I        +ER  G        + L L EFG     G+     +    +  + +K 
Sbjct: 307 MPAI--------WERHFG---QFAGTHALLLGEFGGKYGEGDARDKTWQDALVKYLRSKG 362

Query: 362 IDWALWAFQGSYMYRQVHQDLDESFGVMDHSWTKDRNPKL 378
           I+      QG   Y   + +  ++ G++   WT  R  K+
Sbjct: 367 IN------QG--FYWSWNPNSGDTGGILRDDWTSVRQDKM 362

BLAST of Tan0010405 vs. NCBI nr
Match: XP_038907081.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 908.3 bits (2346), Expect = 3.1e-260
Identity = 426/530 (80.38%), Postives = 480/530 (90.57%), Query Frame = 0

Query: 1   MSKTLFPLIALILFSLLALSQSLPLSTRGRWIVDARTGQRVKLTCVNLVSHAQSMVAQGL 60
           M+K L  LI+L+LFSLL LSQSLPLST GRWIVD++TGQRVKL+CVNLVSHAQSM+AQGL
Sbjct: 1   MAKAL-SLISLVLFSLLVLSQSLPLSTSGRWIVDSQTGQRVKLSCVNLVSHAQSMLAQGL 60

Query: 61  DKRPLKELAAEIKSR-FNCVRLTWSIHMFTRYTFETIGDVFDNLDLASVKSGVQKHNPQI 120
           DKR LK+LA +I SR FNCVRLTWSIHMFTR+ +ETIGDV D LD+  VKSGVQKHNP+I
Sbjct: 61  DKRSLKDLANDISSRDFNCVRLTWSIHMFTRHAYETIGDVLDGLDIGKVKSGVQKHNPEI 120

Query: 121 LNMTVAYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLS 180
           LNMTV++AF+TVI GLG+ G+M++LDNHI+QPRWCCS NDGNGFFGDRNFNP EWL+GL+
Sbjct: 121 LNMTVSHAFRTVINGLGNAGLMIVLDNHISQPRWCCSLNDGNGFFGDRNFNPNEWLQGLT 180

Query: 181 YVASHFTWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLNYDN 240
           YVA HF+  PHVIGMSLRNELRGS  N D W+KY+  GSHL+H INP+LLV+ISGLNYDN
Sbjct: 181 YVARHFSCQPHVIGMSLRNELRGS-NNVDEWHKYVRRGSHLIHNINPNLLVVISGLNYDN 240

Query: 241 DLSHIKKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFEREAGFVVD 300
           DLSH+KKKPLGY L+NKVVLEAHLYSFSG+ E KFV +PLNI C+Q+M+KFEREAGFVVD
Sbjct: 241 DLSHLKKKPLGYKLHNKVVLEAHLYSFSGDPESKFVKKPLNIACNQVMEKFEREAGFVVD 300

Query: 301 MEKPYPLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQVHQDLD 360
           ME PYPL++SEFGYD RGGNKAQNRFM+CFLAR+  KDIDWALWAFQGSYMYRQ  +D D
Sbjct: 301 MEDPYPLWISEFGYDVRGGNKAQNRFMNCFLARLIGKDIDWALWAFQGSYMYRQGQEDRD 360

Query: 361 ESFGVMDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCVKSDGKGGIE 420
           ESFGVMD++WTKDR+P+LKQMLQL KR+NQDPNSKA MSYIMLHPV+GQCVKSDGKGGIE
Sbjct: 361 ESFGVMDNTWTKDRSPQLKQMLQLAKRINQDPNSKASMSYIMLHPVSGQCVKSDGKGGIE 420

Query: 421 LGDCTAPTQWSHGGDASPVKLQSNGQCLKSAGDGKPPVVSADCSGDGSNWTVASKAKLQL 480
           LGDCT PT W+H GD +P+KL SNGQCLKSAGDGKPP+VSADCSGDGSNWTVASKAKLQL
Sbjct: 421 LGDCTTPTLWNHTGDGTPMKLLSNGQCLKSAGDGKPPMVSADCSGDGSNWTVASKAKLQL 480

Query: 481 ATKIGGDQFCLEKESDTSIVMKKCICLTNEWNCFDDPQSQWFKLVPTNVA 530
           ATK GG+ FCLEKESDTSIV+KKCICL +EWNCFDDPQSQWFKLVPTNVA
Sbjct: 481 ATKSGGENFCLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 528

BLAST of Tan0010405 vs. NCBI nr
Match: XP_004143963.2 (glycosyl hydrolase 5 family protein [Cucumis sativus])

HSP 1 Score: 897.5 bits (2318), Expect = 5.5e-257
Identity = 422/525 (80.38%), Postives = 470/525 (89.52%), Query Frame = 0

Query: 6   FPLIALILFSLLALSQSLPLSTRGRWIVDARTGQRVKLTCVNLVSHAQSMVAQGLDKRPL 65
           FPLI+++  SLL LSQSLPLSTRGRWIVD++TGQRVKL+CVNLVSHAQSMVAQGLDKRPL
Sbjct: 5   FPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLDKRPL 64

Query: 66  KELAAEIKSR-FNCVRLTWSIHMFTRYTFETIGDVFDNLDLASVKSGVQKHNPQILNMTV 125
           K+LA EI SR +NCVRLTWS++MFTRY FETIGDV D LD+  VK+GV+KHNPQ LNMTV
Sbjct: 65  KDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFLNMTV 124

Query: 126 AYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLSYVASH 185
             AFKTV++GLG+ G+MVILDNHI+QPRWCCS +DGNGFFGDRNF P EWL+GL+YVA H
Sbjct: 125 TSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAYVARH 184

Query: 186 FTWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLNYDNDLSHI 245
           F+W+P V+GMSLRNELRGS  N  VW KY+  GSHL+H+INP LLV+ISGLNYDNDLS++
Sbjct: 185 FSWHPKVVGMSLRNELRGS-NNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDLSYL 244

Query: 246 KKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFEREAGFVVDMEKPY 305
           KKKPLGYNLNNKVVLEAHLYSFSGE E KFV +PLNI C+Q+MDKFEREAGFVVDM+ PY
Sbjct: 245 KKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMKDPY 304

Query: 306 PLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQVHQDLDESFGV 365
           PLFLSEFGYD RGGNKAQNRFMSCFLARI  KDIDWA WAFQGSYMYRQ  QD+DESFG+
Sbjct: 305 PLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDESFGI 364

Query: 366 MDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCVKSDGKGGIELGDCT 425
           MD SWTKDR+P+L+QMLQL KR+NQDPNSK PMSYIMLHPV+GQCVK DGKGGIELGDC 
Sbjct: 365 MDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELGDCE 424

Query: 426 APTQWSHGGDASPVKLQSNGQCLKSAGDGKPPVVSADCSGDGSNWTVASKAKLQLATKIG 485
            PT W H GD SP+KL  NGQCLKSAGDGKPPVVSA+CSGDGS+WTVASKAKLQL+TK G
Sbjct: 425 TPTLWDHTGDGSPMKLW-NGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLSTKSG 484

Query: 486 GDQFCLEKESDTSIVMKKCICLTNEWNCFDDPQSQWFKLVPTNVA 530
           G+  CLEKESDTSIV+KKCICL +EWNCFDDPQSQWFKLVPTNVA
Sbjct: 485 GENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 527

BLAST of Tan0010405 vs. NCBI nr
Match: KAG6601845.1 (hypothetical protein SDJN03_07078, partial [Cucurbita argyrosperma subsp. sororia] >KAG6601853.1 hypothetical protein SDJN03_07086, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 896.0 bits (2314), Expect = 1.6e-256
Identity = 421/525 (80.19%), Postives = 472/525 (89.90%), Query Frame = 0

Query: 6   FPLIALILFSLLALSQSLPLSTRGRWIVDARTGQRVKLTCVNLVSHAQSMVAQGLDKRPL 65
           F LIALILFSL + SQSLPL TRGRWI+DA+TG+RVKL CVNLVSHAQ+MVAQGLDKRPL
Sbjct: 5   FSLIALILFSLFSFSQSLPLCTRGRWIIDAQTGKRVKLNCVNLVSHAQTMVAQGLDKRPL 64

Query: 66  KELAAEIKSR-FNCVRLTWSIHMFTRYTFETIGDVFDNLDLASVKSGVQKHNPQILNMTV 125
           KELAAEI SR FNCVRLTWS+HMFTRY +ETIGDV D LD+  VK+GV+KHNP+IL M+V
Sbjct: 65  KELAAEISSRQFNCVRLTWSVHMFTRYAYETIGDVLDGLDIGDVKNGVEKHNPEILKMSV 124

Query: 126 AYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLSYVASH 185
           A AF+TVI  LGSEGIMVILDNHI+QPRWCCS +DGNGFFGDRNFNP EW +GLS+VA+ 
Sbjct: 125 AKAFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNEWFQGLSFVATQ 184

Query: 186 FTWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLNYDNDLSHI 245
           FT NPHVIGMSLRNELRG  TN D W  YM +GS L+H+INP++L+IISGLNYDNDLS++
Sbjct: 185 FTCNPHVIGMSLRNELRGPFTNNDAWCYYMRQGSQLIHRINPNILIIISGLNYDNDLSYL 244

Query: 246 KKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFEREAGFVVDMEKPY 305
           KK+PLGY L+NKVVLEAHLYSFSG+NE KFV +PLNI+C++IM+KFEREAGFVVDME PY
Sbjct: 245 KKRPLGYTLHNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFEREAGFVVDMENPY 304

Query: 306 PLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQVHQDLDESFGV 365
           PLFLSEFGYDQ GGN+A+NRFMSCFLARIA KDIDWALWAFQGSYMYRQ H D DESFGV
Sbjct: 305 PLFLSEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQGHTDPDESFGV 364

Query: 366 MDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCVKSDGKGGIELGDCT 425
           MDHSWTKDRNPKL Q+LQL KR+NQDPNSKAPMSYIMLH V+GQCV+SD KGGIELGDCT
Sbjct: 365 MDHSWTKDRNPKLAQLLQLVKRINQDPNSKAPMSYIMLHSVSGQCVRSDNKGGIELGDCT 424

Query: 426 APTQWSHGGDASPVKLQSNGQCLKSAGDGKPPVVSADCSGDGSNWTVASKAKLQLATKIG 485
           +PTQW+H GDASP+ L SNGQCLKSAGDGK P+VSA+CSGDGSNWTV+SKAKLQLATKIG
Sbjct: 425 SPTQWNHTGDASPMMLLSNGQCLKSAGDGKSPMVSAECSGDGSNWTVSSKAKLQLATKIG 484

Query: 486 GDQFCLEKESDTSIVMKKCICLTNEWNCFDDPQSQWFKLVPTNVA 530
            D FCLEKES+ SIV+KKCICL  E +C DDPQ+QWFKLVPTNV+
Sbjct: 485 EDNFCLEKESNASIVLKKCICLDEE-SCMDDPQTQWFKLVPTNVS 528

BLAST of Tan0010405 vs. NCBI nr
Match: XP_008437726.1 (PREDICTED: uncharacterized protein LOC103483073 [Cucumis melo] >KAA0042708.1 major extracellular endoglucanase-like [Cucumis melo var. makuwa] >TYK06112.1 major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 864.0 bits (2231), Expect = 6.7e-247
Identity = 410/525 (78.10%), Postives = 465/525 (88.57%), Query Frame = 0

Query: 6   FPLIALILFSLLALSQSLPLSTRGRWIVDARTGQRVKLTCVNLVSHAQSMVAQGLDKRPL 65
           F LI+L+  SLL LSQSLPLSTRGRWIVD++TGQRVKL+CVNLVSHAQSMVAQGLDKRPL
Sbjct: 5   FSLISLLFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLDKRPL 64

Query: 66  KELAAEIKSR-FNCVRLTWSIHMFTRYTFETIGDVFDNLDLASVKSGVQKHNPQILNMTV 125
           K+LA EI SR +NCVRLTWS++MFTR+ FETIGDV D LD+  VK+GV+KHNPQILNMTV
Sbjct: 65  KDLANEIVSRDYNCVRLTWSVYMFTRHPFETIGDVLDGLDINKVKNGVKKHNPQILNMTV 124

Query: 126 AYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLSYVASH 185
             AFKTV++GLG+ G+MVI+DNHI+QPRWCCS +DGNGFFGDRNF P EWL+GL++    
Sbjct: 125 TSAFKTVVDGLGNAGLMVIMDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAF---- 184

Query: 186 FTWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLNYDNDLSHI 245
                 V+GMSLRNELRGS  N DVW KY+ +GSHL+H+INP LLV+ISGLNYDNDLS++
Sbjct: 185 ------VVGMSLRNELRGS-NNVDVWRKYVKQGSHLIHRINPRLLVVISGLNYDNDLSYL 244

Query: 246 KKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFEREAGFVVDMEKPY 305
           KKKPLGYNL+NKVVLEAHLYSFSGE E KFV +PLNI C+Q+MDKFEREAGFVVDM+ PY
Sbjct: 245 KKKPLGYNLHNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMKNPY 304

Query: 306 PLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQVHQDLDESFGV 365
           PLFLSEFGYD RG NKAQNRFMSCFLARI  KDI+WA WAFQGSYMYRQ  QD+DESFG+
Sbjct: 305 PLFLSEFGYDLRGVNKAQNRFMSCFLARIIGKDIEWAYWAFQGSYMYRQGQQDVDESFGI 364

Query: 366 MDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCVKSDGKGGIELGDCT 425
           MD +WTKDR+P+L+QML+L KR+NQDPNSKAP+SYIMLHPV+GQCV+ DGKGGIELGDC 
Sbjct: 365 MDSTWTKDRSPRLQQMLKLAKRINQDPNSKAPLSYIMLHPVSGQCVQLDGKGGIELGDCE 424

Query: 426 APTQWSHGGDASPVKLQSNGQCLKSAGDGKPPVVSADCSGDGSNWTVASKAKLQLATKIG 485
            PT W H GD SP+KL SNGQCLKSAGDGKPPVVSA+CSGDGS+WTVASKAKLQLATK G
Sbjct: 425 KPTLWDHTGDGSPMKL-SNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLATKSG 484

Query: 486 GDQFCLEKESDTSIVMKKCICLTNEWNCFDDPQSQWFKLVPTNVA 530
           G++ CLEKESDTSIV+KKCICL +EWNCFDDPQ QWFKLVPTNVA
Sbjct: 485 GEKICLEKESDTSIVVKKCICLKDEWNCFDDPQPQWFKLVPTNVA 517

BLAST of Tan0010405 vs. NCBI nr
Match: XP_038907078.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 842.4 bits (2175), Expect = 2.1e-240
Identity = 394/525 (75.05%), Postives = 458/525 (87.24%), Query Frame = 0

Query: 6   FPLIALILFSLLALSQSLPLSTRGRWIVDARTGQRVKLTCVNLVSHAQSMVAQGLDKRPL 65
           F LI+L+ FSLL LSQSL LSTRGRWIVD++TGQRVKL+CVNLVSH QSM+ QGLDKRPL
Sbjct: 5   FSLISLVFFSLLPLSQSLTLSTRGRWIVDSQTGQRVKLSCVNLVSHGQSMLTQGLDKRPL 64

Query: 66  KELAAEIKSR-FNCVRLTWSIHMFTRYTFETIGDVFDNLDLASVKSGVQKHNPQILNMTV 125
            +LA +I SR FNCVRLTWSIHMFTRY+  TI DV DNLD+ +VKSGVQ HN ++LNMTV
Sbjct: 65  NDLANDIISRDFNCVRLTWSIHMFTRYSQMTIVDVLDNLDIKNVKSGVQTHNLEMLNMTV 124

Query: 126 AYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLSYVASH 185
           A+AFKTVI+GLG+ G+M++LDNHI+QPRWCCS +DGNGFFGDR+F+  EWL+GL+YVA H
Sbjct: 125 AHAFKTVIDGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFHTVEWLRGLTYVARH 184

Query: 186 FTWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLNYDNDLSHI 245
           ++W+P VIGMSLRNELRG  TN D WYKY+ +GSHL+H+ NP+LLV+ISG+NYDNDLS +
Sbjct: 185 YSWHPQVIGMSLRNELRGPFTNMDKWYKYVRKGSHLIHRENPNLLVMISGINYDNDLSFL 244

Query: 246 KKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFEREAGFVVDMEKPY 305
           KKKPLGYNLNNKVVLEAHLYSFS E E KF+ +PLN+ CD +M++FEREAGFVVDME PY
Sbjct: 245 KKKPLGYNLNNKVVLEAHLYSFSEEPESKFLEKPLNVACDHVMERFEREAGFVVDMENPY 304

Query: 306 PLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQVHQDLDESFGV 365
           PL+LSEFGY+Q G N+AQNRFMSCFLARI  KD+DWALWAFQG+YMYRQ  ++ +E+FGV
Sbjct: 305 PLWLSEFGYNQGGANEAQNRFMSCFLARIIGKDLDWALWAFQGTYMYRQGQENAEETFGV 364

Query: 366 MDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCVKSDGKGGIELGDCT 425
           MD SWTKDRNP+LKQMLQL KR+N DPNS  PM YIMLHPV+GQCV+SD +GGI LGDC 
Sbjct: 365 MDSSWTKDRNPQLKQMLQLAKRINLDPNSNEPMLYIMLHPVSGQCVRSDNEGGIVLGDCL 424

Query: 426 APTQWSHGGDASPVKLQSNGQCLKSAGDGKPPVVSADCSGDGSNWTVASKAKLQLATKIG 485
            PT+WSH GD S ++L S G CLKS GDGKPPVVSADCSGDGS WTVASKAKLQLATK G
Sbjct: 425 TPTRWSHSGDGSLLQL-SEGLCLKSVGDGKPPVVSADCSGDGSIWTVASKAKLQLATKSG 484

Query: 486 GDQFCLEKESDTSIVMKKCICLTNEWNCFDDPQSQWFKLVPTNVA 530
           G+ FCLEK+SD+ IV+KKCICL +E NCFDDPQSQWFKLVPTNVA
Sbjct: 485 GENFCLEKKSDSKIVVKKCICLKDELNCFDDPQSQWFKLVPTNVA 528

BLAST of Tan0010405 vs. ExPASy TrEMBL
Match: A0A0A0KNT6 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G151630 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.7e-257
Identity = 422/525 (80.38%), Postives = 470/525 (89.52%), Query Frame = 0

Query: 6   FPLIALILFSLLALSQSLPLSTRGRWIVDARTGQRVKLTCVNLVSHAQSMVAQGLDKRPL 65
           FPLI+++  SLL LSQSLPLSTRGRWIVD++TGQRVKL+CVNLVSHAQSMVAQGLDKRPL
Sbjct: 5   FPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLDKRPL 64

Query: 66  KELAAEIKSR-FNCVRLTWSIHMFTRYTFETIGDVFDNLDLASVKSGVQKHNPQILNMTV 125
           K+LA EI SR +NCVRLTWS++MFTRY FETIGDV D LD+  VK+GV+KHNPQ LNMTV
Sbjct: 65  KDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFLNMTV 124

Query: 126 AYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLSYVASH 185
             AFKTV++GLG+ G+MVILDNHI+QPRWCCS +DGNGFFGDRNF P EWL+GL+YVA H
Sbjct: 125 TSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAYVARH 184

Query: 186 FTWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLNYDNDLSHI 245
           F+W+P V+GMSLRNELRGS  N  VW KY+  GSHL+H+INP LLV+ISGLNYDNDLS++
Sbjct: 185 FSWHPKVVGMSLRNELRGS-NNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDLSYL 244

Query: 246 KKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFEREAGFVVDMEKPY 305
           KKKPLGYNLNNKVVLEAHLYSFSGE E KFV +PLNI C+Q+MDKFEREAGFVVDM+ PY
Sbjct: 245 KKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMKDPY 304

Query: 306 PLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQVHQDLDESFGV 365
           PLFLSEFGYD RGGNKAQNRFMSCFLARI  KDIDWA WAFQGSYMYRQ  QD+DESFG+
Sbjct: 305 PLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDESFGI 364

Query: 366 MDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCVKSDGKGGIELGDCT 425
           MD SWTKDR+P+L+QMLQL KR+NQDPNSK PMSYIMLHPV+GQCVK DGKGGIELGDC 
Sbjct: 365 MDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELGDCE 424

Query: 426 APTQWSHGGDASPVKLQSNGQCLKSAGDGKPPVVSADCSGDGSNWTVASKAKLQLATKIG 485
            PT W H GD SP+KL  NGQCLKSAGDGKPPVVSA+CSGDGS+WTVASKAKLQL+TK G
Sbjct: 425 TPTLWDHTGDGSPMKLW-NGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLSTKSG 484

Query: 486 GDQFCLEKESDTSIVMKKCICLTNEWNCFDDPQSQWFKLVPTNVA 530
           G+  CLEKESDTSIV+KKCICL +EWNCFDDPQSQWFKLVPTNVA
Sbjct: 485 GENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 527

BLAST of Tan0010405 vs. ExPASy TrEMBL
Match: A0A5A7TIV3 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G002130 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 3.3e-247
Identity = 410/525 (78.10%), Postives = 465/525 (88.57%), Query Frame = 0

Query: 6   FPLIALILFSLLALSQSLPLSTRGRWIVDARTGQRVKLTCVNLVSHAQSMVAQGLDKRPL 65
           F LI+L+  SLL LSQSLPLSTRGRWIVD++TGQRVKL+CVNLVSHAQSMVAQGLDKRPL
Sbjct: 5   FSLISLLFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLDKRPL 64

Query: 66  KELAAEIKSR-FNCVRLTWSIHMFTRYTFETIGDVFDNLDLASVKSGVQKHNPQILNMTV 125
           K+LA EI SR +NCVRLTWS++MFTR+ FETIGDV D LD+  VK+GV+KHNPQILNMTV
Sbjct: 65  KDLANEIVSRDYNCVRLTWSVYMFTRHPFETIGDVLDGLDINKVKNGVKKHNPQILNMTV 124

Query: 126 AYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLSYVASH 185
             AFKTV++GLG+ G+MVI+DNHI+QPRWCCS +DGNGFFGDRNF P EWL+GL++    
Sbjct: 125 TSAFKTVVDGLGNAGLMVIMDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAF---- 184

Query: 186 FTWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLNYDNDLSHI 245
                 V+GMSLRNELRGS  N DVW KY+ +GSHL+H+INP LLV+ISGLNYDNDLS++
Sbjct: 185 ------VVGMSLRNELRGS-NNVDVWRKYVKQGSHLIHRINPRLLVVISGLNYDNDLSYL 244

Query: 246 KKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFEREAGFVVDMEKPY 305
           KKKPLGYNL+NKVVLEAHLYSFSGE E KFV +PLNI C+Q+MDKFEREAGFVVDM+ PY
Sbjct: 245 KKKPLGYNLHNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMKNPY 304

Query: 306 PLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQVHQDLDESFGV 365
           PLFLSEFGYD RG NKAQNRFMSCFLARI  KDI+WA WAFQGSYMYRQ  QD+DESFG+
Sbjct: 305 PLFLSEFGYDLRGVNKAQNRFMSCFLARIIGKDIEWAYWAFQGSYMYRQGQQDVDESFGI 364

Query: 366 MDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCVKSDGKGGIELGDCT 425
           MD +WTKDR+P+L+QML+L KR+NQDPNSKAP+SYIMLHPV+GQCV+ DGKGGIELGDC 
Sbjct: 365 MDSTWTKDRSPRLQQMLKLAKRINQDPNSKAPLSYIMLHPVSGQCVQLDGKGGIELGDCE 424

Query: 426 APTQWSHGGDASPVKLQSNGQCLKSAGDGKPPVVSADCSGDGSNWTVASKAKLQLATKIG 485
            PT W H GD SP+KL SNGQCLKSAGDGKPPVVSA+CSGDGS+WTVASKAKLQLATK G
Sbjct: 425 KPTLWDHTGDGSPMKL-SNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLATKSG 484

Query: 486 GDQFCLEKESDTSIVMKKCICLTNEWNCFDDPQSQWFKLVPTNVA 530
           G++ CLEKESDTSIV+KKCICL +EWNCFDDPQ QWFKLVPTNVA
Sbjct: 485 GEKICLEKESDTSIVVKKCICLKDEWNCFDDPQPQWFKLVPTNVA 517

BLAST of Tan0010405 vs. ExPASy TrEMBL
Match: A0A1S3AUC3 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103483073 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 3.3e-247
Identity = 410/525 (78.10%), Postives = 465/525 (88.57%), Query Frame = 0

Query: 6   FPLIALILFSLLALSQSLPLSTRGRWIVDARTGQRVKLTCVNLVSHAQSMVAQGLDKRPL 65
           F LI+L+  SLL LSQSLPLSTRGRWIVD++TGQRVKL+CVNLVSHAQSMVAQGLDKRPL
Sbjct: 5   FSLISLLFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLDKRPL 64

Query: 66  KELAAEIKSR-FNCVRLTWSIHMFTRYTFETIGDVFDNLDLASVKSGVQKHNPQILNMTV 125
           K+LA EI SR +NCVRLTWS++MFTR+ FETIGDV D LD+  VK+GV+KHNPQILNMTV
Sbjct: 65  KDLANEIVSRDYNCVRLTWSVYMFTRHPFETIGDVLDGLDINKVKNGVKKHNPQILNMTV 124

Query: 126 AYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLSYVASH 185
             AFKTV++GLG+ G+MVI+DNHI+QPRWCCS +DGNGFFGDRNF P EWL+GL++    
Sbjct: 125 TSAFKTVVDGLGNAGLMVIMDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAF---- 184

Query: 186 FTWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLNYDNDLSHI 245
                 V+GMSLRNELRGS  N DVW KY+ +GSHL+H+INP LLV+ISGLNYDNDLS++
Sbjct: 185 ------VVGMSLRNELRGS-NNVDVWRKYVKQGSHLIHRINPRLLVVISGLNYDNDLSYL 244

Query: 246 KKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFEREAGFVVDMEKPY 305
           KKKPLGYNL+NKVVLEAHLYSFSGE E KFV +PLNI C+Q+MDKFEREAGFVVDM+ PY
Sbjct: 245 KKKPLGYNLHNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMKNPY 304

Query: 306 PLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQVHQDLDESFGV 365
           PLFLSEFGYD RG NKAQNRFMSCFLARI  KDI+WA WAFQGSYMYRQ  QD+DESFG+
Sbjct: 305 PLFLSEFGYDLRGVNKAQNRFMSCFLARIIGKDIEWAYWAFQGSYMYRQGQQDVDESFGI 364

Query: 366 MDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCVKSDGKGGIELGDCT 425
           MD +WTKDR+P+L+QML+L KR+NQDPNSKAP+SYIMLHPV+GQCV+ DGKGGIELGDC 
Sbjct: 365 MDSTWTKDRSPRLQQMLKLAKRINQDPNSKAPLSYIMLHPVSGQCVQLDGKGGIELGDCE 424

Query: 426 APTQWSHGGDASPVKLQSNGQCLKSAGDGKPPVVSADCSGDGSNWTVASKAKLQLATKIG 485
            PT W H GD SP+KL SNGQCLKSAGDGKPPVVSA+CSGDGS+WTVASKAKLQLATK G
Sbjct: 425 KPTLWDHTGDGSPMKL-SNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLATKSG 484

Query: 486 GDQFCLEKESDTSIVMKKCICLTNEWNCFDDPQSQWFKLVPTNVA 530
           G++ CLEKESDTSIV+KKCICL +EWNCFDDPQ QWFKLVPTNVA
Sbjct: 485 GEKICLEKESDTSIVVKKCICLKDEWNCFDDPQPQWFKLVPTNVA 517

BLAST of Tan0010405 vs. ExPASy TrEMBL
Match: A0A6J1JQD4 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111487386 PE=3 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 1.2e-236
Identity = 388/476 (81.51%), Postives = 429/476 (90.13%), Query Frame = 0

Query: 55  MVAQGLDKRPLKELAAEIKSR-FNCVRLTWSIHMFTRYTFETIGDVFDNLDLASVKSGVQ 114
           MVAQGLDKRPLKELAAEI SR FNCVRLTWSIHMFTRY +ETIGDV D LD+  VK+GV+
Sbjct: 1   MVAQGLDKRPLKELAAEISSRQFNCVRLTWSIHMFTRYAYETIGDVLDGLDIGDVKNGVE 60

Query: 115 KHNPQILNMTVAYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQE 174
           KHNP+IL M+V  AF+TVI  LGSEGIMVILDNHI+QPRWCCS +DGNGFFGDRNFNP E
Sbjct: 61  KHNPEILKMSVVKAFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 175 WLKGLSYVASHFTWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIIS 234
           WL+GLS+VA+ FT NPHVIGMSLRNELRG  TN D W  YM +GS L+H+INP++LVIIS
Sbjct: 121 WLQGLSFVATQFTCNPHVIGMSLRNELRGPFTNNDAWCYYMRQGSQLIHRINPNILVIIS 180

Query: 235 GLNYDNDLSHIKKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFERE 294
           GLNYDNDLS++KK+PLGY L+NKVVLEAHLYSFSG+NE KFV +PLNI+C++IM+KFERE
Sbjct: 181 GLNYDNDLSYLKKRPLGYTLHNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 240

Query: 295 AGFVVDMEKPYPLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQ 354
           AGFVVDME PYPLFLSEFGYDQ GGN+A+NRFMSCFLARIA KDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMENPYPLFLSEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 300

Query: 355 VHQDLDESFGVMDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCVKSD 414
            H D DESFGVMDHSWTKDRNPKL Q+LQL KR+NQDPNSKAPMSYI+LH V+GQCVKSD
Sbjct: 301 GHTDPDESFGVMDHSWTKDRNPKLAQLLQLVKRINQDPNSKAPMSYIVLHSVSGQCVKSD 360

Query: 415 GKGGIELGDCTAPTQWSHGGDASPVKLQSNGQCLKSAGDGKPPVVSADCSGDGSNWTVAS 474
            KGGIELGDCTAPTQW+H GDASP+KL SNGQCLKSAGDGK PVVSA+CSGDGSNWTV+S
Sbjct: 361 NKGGIELGDCTAPTQWNHTGDASPMKLLSNGQCLKSAGDGKSPVVSAECSGDGSNWTVSS 420

Query: 475 KAKLQLATKIGGDQFCLEKESDTSIVMKKCICLTNEWNCFDDPQSQWFKLVPTNVA 530
           KAKLQLATKIG D FCLEKESD SIV+KKCICL  E +C DDPQ+QWFKLVPTNV+
Sbjct: 421 KAKLQLATKIGEDNFCLEKESDASIVLKKCICLDEE-SCMDDPQTQWFKLVPTNVS 475

BLAST of Tan0010405 vs. ExPASy TrEMBL
Match: A0A6J1GZR4 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459103 PE=3 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 5.4e-234
Identity = 382/476 (80.25%), Postives = 423/476 (88.87%), Query Frame = 0

Query: 55  MVAQGLDKRPLKELAAEIKSR-FNCVRLTWSIHMFTRYTFETIGDVFDNLDLASVKSGVQ 114
           MVAQGLDKRPLKELA+EI SR FNCVRLTWS+HMFTRY +E +GDV D LD+A VKSGV 
Sbjct: 1   MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 60

Query: 115 KHNPQILNMTVAYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQE 174
           KHNP+IL MT+A AF+ VI  LGSEGIMVILDNHI+QPRWCCS +DGNGFFGDRNFNP E
Sbjct: 61  KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 175 WLKGLSYVASHFTWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIIS 234
           WL+GLS+VA+ FT NPHV+GMSLRNELRG  TN D W  YM  GS L+H+INP+LL+IIS
Sbjct: 121 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 180

Query: 235 GLNYDNDLSHIKKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFERE 294
           GLNYDNDLS +KK+PLGY   NKVVLEAHLYSFSG+NE KFV +PLNI+C++IM+KFERE
Sbjct: 181 GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 240

Query: 295 AGFVVDMEKPYPLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQ 354
           AGFVVDM+ PYPLF+SEFGYDQ GGN+A+NRFMSCFLARIA KDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 300

Query: 355 VHQDLDESFGVMDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCVKSD 414
            H+D DESFGVMDHSWTKDRNPKLKQMLQL KR+NQD NSKAPMSYI+LHPV+GQCVKSD
Sbjct: 301 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD 360

Query: 415 GKGGIELGDCTAPTQWSHGGDASPVKLQSNGQCLKSAGDGKPPVVSADCSGDGSNWTVAS 474
           GKGGIELGDC  PTQW+H GDASP+KL SNGQCLKSAGDGK P+VS DCSGD  NWTVAS
Sbjct: 361 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 420

Query: 475 KAKLQLATKIGGDQFCLEKESDTSIVMKKCICLTNEWNCFDDPQSQWFKLVPTNVA 530
           KAKLQL+TK+G D FCLEKESDTSIV+KKCICL  E +C DDPQSQWFKLVPTNVA
Sbjct: 421 KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAE-SCMDDPQSQWFKLVPTNVA 475

BLAST of Tan0010405 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 389.8 bits (1000), Expect = 3.5e-108
Identity = 222/532 (41.73%), Postives = 304/532 (57.14%), Query Frame = 0

Query: 6   FPLIALILFSLLALSQSLPLSTRGRWIV-DARTGQRVKLTCVNLVSHAQSMVAQGLDKRP 65
           F  I++ L   +  + + P ST  RWIV D   G+RVKLTCVN  SH ++ VA+GL K+P
Sbjct: 4   FFFISVFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQP 63

Query: 66  LKELAAEIKSR-FNCVRLTWSIHMFTRYTFE---TIGDVFDNLDLASVKSGVQKHNPQIL 125
           L  +A +I S  FNCVRLTW +++ T  +F    T+        L    SG Q HNP IL
Sbjct: 64  LDAIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTIL 123

Query: 126 NMTVAYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLSY 185
           ++ +  AF+ V+  L    +MVILDNHI+QP WCCS NDGNGFFGD++ NPQ W+KGL  
Sbjct: 124 DLPLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKK 183

Query: 186 VASHF-TWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLNYDN 245
           +AS F   + +V+GMSLRNELRG   N   WYKYM EG+  VH +NP++LVI+SGLNY  
Sbjct: 184 MASMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYAT 243

Query: 246 DLSHIKKKPLGYNLNNKVVLEAHLYSF----SGENEKKFVNRPLNIVCDQIMDKFEREAG 305
           DLS ++++P   +   KVV E H Y F     G+N        LN +C +  +K  + +G
Sbjct: 244 DLSFLRERPFEVSFRRKVVFEIHWYGFWNTWEGDN--------LNKICGKETEKMMKMSG 303

Query: 306 FVVDMEKPYPLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQVH 365
           F+  +EK  PLF+SEFG DQRG N   N+F+SCF+A  A +D+DW+LW   GSY  R+  
Sbjct: 304 FL--LEKGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKS 363

Query: 366 QDLDESFGVMDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCVKSDGK 425
              DES+GV+D +W+  RN  + QM+   +          P   IM HP TG C+     
Sbjct: 364 IGSDESYGVLDFNWSSIRNSTILQMISAIQTPFIGLMETQPKK-IMFHPSTGLCIVRKSL 423

Query: 426 GGIELGDCTAPTQWSHGGDASPVKLQSNGQCLKSAGDGKPPVVSADCSGD-GSNWTVASK 485
             ++LG C     W           +    CLK+   GK   +    S    S W + S 
Sbjct: 424 FQLKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFSESYCSKWKLFSD 483

Query: 486 AKLQLAT-KIGGDQFCLEKESD-TSIVMKKCICLTNEWNCFDDPQSQWFKLV 525
           +K+QL++    G   CL+ +++  +IV   C CL    +C  DP+SQWFKLV
Sbjct: 484 SKMQLSSITKNGFSVCLDVDTENNNIVTNSCKCLRGNSSC--DPRSQWFKLV 522

BLAST of Tan0010405 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 386.0 bits (990), Expect = 5.0e-107
Identity = 200/509 (39.29%), Postives = 291/509 (57.17%), Query Frame = 0

Query: 24  PLSTRGRWIVDARTGQRVKLTCVNLVSHAQSMVAQGLDKRPLKELAAEIKSR-FNCVRLT 83
           PLST  RWI+D + GQRVKL CVN  SH Q +VA+GL K+ + +LA +I +  FNCVR T
Sbjct: 4   PLSTNSRWIIDEK-GQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 84  WSIHMFTRYTFE---TIGDVFDNLDLASVKSGVQKHNPQILNMTVAYAFKTVIEGLGSEG 143
           W + + T  T     T+   F +L L    SG +  NP ++++ +  A+K V+  LG+  
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 144 IMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLSYVASHFTWNPHVIGMSLRNE 203
           +MVILDNH+T+P WCC YNDGNGFFGD  F+P  W+ GL+ +A  F    +V+GMSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 204 LRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLNYDNDLSHIKKKPLGYNLNNKVVL 263
           LRG   N D W+KYM +G+  VH+ NP++LVI+SGL+YD DLS ++ + +      K+V 
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNLTFTRKLVF 243

Query: 264 EAHLYSFSGENEKKFVNRPLNIVCDQIMDKFEREAGFVVDMEKPYPLFLSEFGYDQRGGN 323
           E H YSF+  N   + ++  N  C +I+   E   GF +   + +P+FLSEFG D RG N
Sbjct: 244 ELHRYSFT--NTNTWSSKNPNEACGEILKSIENGGGFNL---RDFPVFLSEFGIDLRGKN 303

Query: 324 KAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQVHQDLDESFGVMDHSWTKDRNPKLKQ 383
              NR++ C L   A  D+DW++W  QGSY  R+    + E +G++D  W + R+    Q
Sbjct: 304 VNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFLQ 363

Query: 384 MLQLPKRLNQDPNSKAPMSYIMLHPVTGQCVKSD--GKGGIELGDCTAPTQWSHGGDASP 443
            L L     Q P S++ +  ++ HP+TG C+         + LG C     WS+    + 
Sbjct: 364 RLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSY-TPQNT 423

Query: 444 VKLQSNGQCLKSAGDGKPPVVS-ADCSGDG-SNWTVASKAKLQLATKIGGDQFCLEKESD 503
           + L+    CL+S G   P  +S   CS    S W   S + + LA K   +  CL+ +  
Sbjct: 424 LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAAKSTNNSLCLDVDET 483

Query: 504 TSIVMKKCICLTNEWNCFDDPQSQWFKLV 525
            +++   C C+  E +   DP SQWFK+V
Sbjct: 484 NNLMASNCKCVKGE-DSSCDPISQWFKIV 504

BLAST of Tan0010405 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 383.3 bits (983), Expect = 3.2e-106
Identity = 207/532 (38.91%), Postives = 302/532 (56.77%), Query Frame = 0

Query: 5   LFPLIALILFSLLAL----SQSLPLSTRGRWIVDARTGQRVKLTCVNLVSHAQSMVAQGL 64
           +FP      FS +A     + S PLST  RWIVD   G RVKL C N  SH Q +VA+GL
Sbjct: 12  IFPFFC-FFFSFIAQNTVPNMSYPLSTSSRWIVD-ENGLRVKLVCANWPSHLQPVVAEGL 71

Query: 65  DKRPLKELAAEI-KSRFNCVRLTWSIHMFTRYTFE---TIGDVFDNLDLASVKSGVQKHN 124
            K+P+  +A +I +  FNCVRLTW + + T  T     T+   F +L L     G Q +N
Sbjct: 72  SKQPVDAVAKKIVEMGFNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNN 131

Query: 125 PQILNMTVAYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLK 184
           P I+++ +  A+KTV+  LG+  +MVILDNH+T+P WCC+ +DGNGFFGD+ F+P  W+ 
Sbjct: 132 PSIIDLPLIEAYKTVVTTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVA 191

Query: 185 GLSYVASHFTWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLN 244
            L  +A+ F    +V+GMSLRNELRG   N + W+KYM +G+  VH  N  +LVI+SGL+
Sbjct: 192 ALKKMAATFNGVSNVVGMSLRNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLS 251

Query: 245 YDNDLSHIKKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFEREAGF 304
           +D DLS ++ +P+  +   K+V E H YSFS  N     N P N +C +++++     G+
Sbjct: 252 FDADLSFVRSRPVKLSFTGKLVFELHWYSFSDGNSWA-ANNP-NDICGRVLNRIGNGGGY 311

Query: 305 VVDMEKPYPLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQVHQ 364
           +  + + +PLFLSEFG D+RG N   NR+  C     A  D+DW+LWA  GSY  RQ   
Sbjct: 312 L--LNQGFPLFLSEFGIDERGVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKV 371

Query: 365 DLDESFGVMDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCV--KSDG 424
            ++E +GV+D  W   RN    Q +   +   Q P  +     ++ HP+TG C+    D 
Sbjct: 372 GMNEYYGVLDSDWISVRNSSFLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCIVRSLDD 431

Query: 425 KGGIELGDCTAPTQWSHGGDASPVKLQSNGQCLKSAGDGKPPVVS-ADCSGDGSNWTVAS 484
              + LG C +   WS+   A  +K Q    CL+S G   P  ++   CS  GS W   S
Sbjct: 432 PKMLTLGPCNSSEPWSYTKKALRIKDQQ--LCLQSNGPKNPVTMTRTSCSTSGSKWQTIS 491

Query: 485 KAKLQLA-TKIGGDQFCLEKESDTSIVMKKCICLTNEWNCFDDPQSQWFKLV 525
            +++ LA T       CL+ ++  ++V   C CL+ + +C  +P SQWFK++
Sbjct: 492 ASRMHLASTTSNKTSLCLDVDTANNVVANACKCLSKDKSC--EPMSQWFKII 533

BLAST of Tan0010405 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 362.1 bits (928), Expect = 7.8e-100
Identity = 207/529 (39.13%), Postives = 298/529 (56.33%), Query Frame = 0

Query: 4   TLFPLIALILFSLLALSQSLPLSTRGRWIVDARTGQRVKLTCVNLVSHAQSMVAQGLDKR 63
           T+F  + L L S L L+   PL T+ RWIV+ + G RVKL C N  SH + +VA+GL  +
Sbjct: 8   TVFLFLFLSLIS-LTLATDYPLFTKSRWIVNNK-GHRVKLACANWPSHLKPVVAEGLSSQ 67

Query: 64  PLKELAAEIKSR-FNCVRLTWSIHMFTRYTFE---TIGDVFDNLDLASVKSGVQKHNPQI 123
           P+  ++ +IK   FNCVRLTW + +    T     T+   F+   L     G+  HNP I
Sbjct: 68  PMDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHNPYI 127

Query: 124 LNMTVAYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLS 183
           +N  +   F+ V+  LG   +MVILDNH T P WCCS +D + FFGD  FNP  W+ GL 
Sbjct: 128 VNTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWMLGLK 187

Query: 184 YVASHFTWNPHVIGMSLRNELRG-SLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLNYD 243
            +A+ F    +V+GMSLRNELRG + T+KD WYKYM +G+  VH  NP++LVI+SGLN+D
Sbjct: 188 KMATIFMNVKNVVGMSLRNELRGYNHTSKD-WYKYMQKGAEAVHTSNPNVLVILSGLNFD 247

Query: 244 NDLSHIKKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFEREAGFVV 303
            DLS +K +P+  +   K+VLE H YSF+ +   ++ +  +N  C Q+  K  R  GFV+
Sbjct: 248 ADLSFLKDRPVNLSFKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSKERRTGGFVL 307

Query: 304 DMEKPYPLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQVHQDL 363
           D  + +PLFLSEFG DQRGG+   NR+M+C LA  A KD+DWA+WA  G Y +R+  + +
Sbjct: 308 D--QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGKRGV 367

Query: 364 DESFGVMDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCVKSDG---K 423
            E++G++D +W    N    + L + +  +  P  K      + HP+TG C+       +
Sbjct: 368 VEAYGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLVRKSHCHE 427

Query: 424 GGIELGDCTAPTQWSHGGDASPVKLQSNGQCLK-SAGDGKPPVVSADCSGDGSNWTVASK 483
             + LG CT    WS+         + +  CL+     GK   +   C+        A+K
Sbjct: 428 SELTLGPCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRICT--KIEQISATK 487

Query: 484 AKLQLATKIGGDQFCLEKESDTSIVMKKCICLTNEWNCFDDPQSQWFKL 524
             L   T   G   CL+ +SD ++V   C CLT +  C  +P SQWFK+
Sbjct: 488 MHLSFNTS-DGSLVCLDVDSDNNVVANSCNCLTGDTTC--EPASQWFKI 525

BLAST of Tan0010405 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 333.6 bits (854), Expect = 3.0e-91
Identity = 202/531 (38.04%), Postives = 285/531 (53.67%), Query Frame = 0

Query: 6   FPLIALILFSLLALSQSLPLSTRGRWIVDARTGQRVKLTCVNLVSHAQSMVAQGLDKRPL 65
           F L  L + S   L+ S PLST+ RWIVD + GQRVKL CVN  +H Q  VA+GL K+PL
Sbjct: 7   FCLFFLFISSTSKLTTSYPLSTKSRWIVDEK-GQRVKLACVNWPAHLQPTVAEGLSKQPL 66

Query: 66  KELAAEIKSR-FNCVRLTWSIHMFTRYTFE---TIGDVFDNLDLASVKSGVQKHNPQILN 125
             ++ +I S  FNCVRLTW + + T  T     T+   F++L L     G+Q HNP++L+
Sbjct: 67  DSISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLH 126

Query: 126 MTVAYAFKTVIEGLGSEGIMVILDNHITQPRWCCSYNDGNGFFGDRNFNPQEWLKGLSYV 185
           + +  AF+ V+  LG  G+MVILDNH+T P WCC  ND + FFG  +F+P  W KGL  +
Sbjct: 127 LPLFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKM 186

Query: 186 ASHFTWNPHVIGMSLRNELRGSLTNKDVWYKYMGEGSHLVHKINPHLLVIISGLNYDNDL 245
           A+ F    HVIGMSLRNE RG+    D+W+++M +G+  VH  NP LLVI+SG+++D +L
Sbjct: 187 ATLFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFDTNL 246

Query: 246 SHIKKKPLGYNLNNKVVLEAHLYSFSGENEKKFVNRPLNIVCDQIMDKFEREAGFVVDME 305
           S ++ + +  +  +K+V E H YSFS +    +     N  C +I++K     GF+  + 
Sbjct: 247 SFLRDRSVNVSFTDKLVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKVTHNGGFL--LG 306

Query: 306 KPYPLFLSEFGYDQRGGNKAQNRFMSCFLARIAAKDIDWALWAFQGSYMYRQVHQDLDES 365
           + +PL LSEFG DQRGG+ + NR+M+C +A  A  D+DWA+WA  G Y  R         
Sbjct: 307 RGFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLR--------- 366

Query: 366 FGVMDHSWTKDRNPKLKQMLQLPKRLNQDPNSKAPMSYIMLHPVTGQCV---KSDGKGGI 425
                        P L+                 P   ++ HP TG CV    SD    +
Sbjct: 367 -----------TGPGLR-----------------PNKNLLFHPSTGLCVTNNPSDNIPTL 426

Query: 426 ELGDCTAPTQWSHGGDASPVKLQSNGQCLKS---AGDGKPPVVSADCSGDGSNWTVASKA 485
            LG C     W+   + S   L  N  C+++    G      V   CS  G      S  
Sbjct: 427 RLGPCPKSDPWTF--NPSEGILWINKMCVEAPNVVGQKVKLGVGTKCSKLGQ----ISAT 486

Query: 486 KLQLATKI-GGDQFCLE-KESDTSIVMKKCICLTNEWNCFDDPQSQWFKLV 525
           K+ L+ K   G   CL+  E D S+V  +C  LT + +C  DP SQWFK++
Sbjct: 487 KMHLSFKTSNGLLLCLDVDERDNSVVANRCKFLTMDASC--DPASQWFKVL 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HLA08.3e-11540.63Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P194872.8e-0924.00Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra... [more]
Match NameE-valueIdentityDescription
XP_038907081.13.1e-26080.38glycosyl hydrolase 5 family protein-like [Benincasa hispida][more]
XP_004143963.25.5e-25780.38glycosyl hydrolase 5 family protein [Cucumis sativus][more]
KAG6601845.11.6e-25680.19hypothetical protein SDJN03_07078, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_008437726.16.7e-24778.10PREDICTED: uncharacterized protein LOC103483073 [Cucumis melo] >KAA0042708.1 maj... [more]
XP_038907078.12.1e-24075.05glycosyl hydrolase 5 family protein-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0KNT62.7e-25780.38Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G151630 PE=3... [more]
A0A5A7TIV33.3e-24778.10Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3AUC33.3e-24778.10Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103483073 PE=3 SV... [more]
A0A6J1JQD41.2e-23681.51Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111487386 PE=... [more]
A0A6J1GZR45.4e-23480.25Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459103 P... [more]
Match NameE-valueIdentityDescription
AT3G26130.13.5e-10841.73Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26140.15.0e-10739.29Cellulase (glycosyl hydrolase family 5) protein [more]
AT1G13130.13.2e-10638.91Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.17.8e-10039.13Glycosyl hydrolase superfamily protein [more]
AT5G16700.13.0e-9138.04Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 22..388
e-value: 2.5E-64
score: 219.7
NoneNo IPR availableGENE3D2.80.10.50coord: 398..513
e-value: 3.1E-6
score: 29.3
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 7..525
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 64..345
e-value: 1.2E-20
score: 74.1
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 23..381

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0010405.1Tan0010405.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds