Tan0010400 (gene) Snake gourd v1

BLAST of Tan0010400 vs. ExPASy Swiss-Prot
Match: Q9ZSM8 (Histone-lysine N-methyltransferase EZA1 OS=Arabidopsis thaliana OX=3702 GN=EZA1 PE=1 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 7.9e-228
Identity = 438/751 (58.32%), Postives = 538/751 (71.64%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MAEDQSVVGRR+IYY+QHG E LICSDSEEE  EPE+EK EFSEGED ++W+I QE+G+G
Sbjct: 145 MAEDQSVVGRRQIYYEQHGGETLICSDSEEE-PEPEEEKREFSEGEDSIIWLIGQEYGMG 204

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
           E+V   L   +    S+I ERYN LK ++ + ++   +   FK   GISL K L + LDS
Sbjct: 205 EEVQDALCQLLSVDASDILERYNELKLKD-KQNTEEFSNSGFK--LGISLEKGLGAALDS 264

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQ-RTQRKTPDQ 180
           FDNLFCRRC++FDCRLHGCSQ LI  SEK  YW ++E +RKPCS  C+LQ +  R+ P+ 
Sbjct: 265 FDNLFCRRCLVFDCRLHGCSQPLISASEKQPYWSDYEGDRKPCSKHCYLQLKAVREVPET 324

Query: 181 RNKRPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGVS--VSEMTVGL-NLDASVGP 240
            +    +SK EE ++  +       + ++  +    + GVS  V +  +G+ N+D+S G 
Sbjct: 325 CSN--FASKAEEKASEEEC-----SKAVSSDVPHAAASGVSLQVEKTDIGIKNVDSSSGV 384

Query: 241 ATNPRNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDTIPELCST 300
                   ++  ++ + +V +  D   + N S   KKQK  +A+D   +  +++P L   
Sbjct: 385 -------EQEHGIRGKREVPILKDSNDLPNLSN--KKQK-TAASDTKMSFVNSVPSL-DQ 444

Query: 301 STKHSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCRTVEVKEL 360
           +   +  D  G    ++ R+  + ++ +G   E IP + +        +S C+       
Sbjct: 445 ALDSTKGDQGGTTDNKVNRDSEADAKEVG---EPIPDNSVHD----GGSSICQPHH---- 504

Query: 361 STSKLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVF 420
             S   A  ++E  E +  S+EW  +EK+LY+KG+EIFGRNSCLI+RNLLSGLKTC++V 
Sbjct: 505 -GSGNGAIIIAEMSETSRPSTEWNPIEKDLYLKGVEIFGRNSCLIARNLLSGLKTCLDVS 564

Query: 421 NYMHNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKLKYSWKSA 480
           NYM     S  + S+  P+   DD G+TD      ++  R+RL R++GK RKLKYS KSA
Sbjct: 565 NYMRENEVSVFRRSS-TPNLLLDD-GRTDPGNDNDEVPPRTRLFRRKGKTRKLKYSTKSA 624

Query: 481 GHPSFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCH 540
           GHPS WKRIA GKNQSCKQYTPCGCLS CGK CPCL N TCCEKYCGCSKSCKNRFRGCH
Sbjct: 625 GHPSVWKRIAGGKNQSCKQYTPCGCLSMCGKDCPCLTNETCCEKYCGCSKSCKNRFRGCH 684

Query: 541 CAKSQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDGQCGNMRLLLRQ 600
           CAKSQCRSRQCPCFAAGRECDPDVCRNCWVSCGDG    LGE PR+G+GQCGNMRLLLRQ
Sbjct: 685 CAKSQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGS---LGEAPRRGEGQCGNMRLLLRQ 744

Query: 601 QQRILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLN 660
           QQRILL KSDVAGWGAFLKN+V+KN+YLGEYTGELISH EADKRGKIYDRANSSFLFDLN
Sbjct: 745 QQRILLGKSDVAGWGAFLKNSVSKNEYLGEYTGELISHHEADKRGKIYDRANSSFLFDLN 804

Query: 661 DQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEELFYDYRYGP 720
           DQYVLDA RKGDKLKFANHS+ PNCYAKVM VAGDHRVGIFA ERIEASEELFYDYRYGP
Sbjct: 805 DQYVLDAQRKGDKLKFANHSAKPNCYAKVMFVAGDHRVGIFANERIEASEELFYDYRYGP 856

Query: 721 DQAPAWARKPEGSKRDDASISQGRAKKHQSH 748
           DQAP WARKPEGSK+DD++I+  RA+KHQSH
Sbjct: 865 DQAPVWARKPEGSKKDDSAITHRRARKHQSH 856

BLAST of Tan0010400 vs. ExPASy Swiss-Prot
Match: Q8S4P4 (Histone-lysine N-methyltransferase EZ3 OS=Zea mays OX=4577 GN=EZ3 PE=2 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 1.9e-221
Identity = 424/760 (55.79%), Postives = 528/760 (69.47%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MA+DQSVVGRRRIYYD  G+EALICSDS+EE+ EPE+EKH F+EGED+++W   QEHGL 
Sbjct: 169 MADDQSVVGRRRIYYDPVGNEALICSDSDEEIPEPEEEKHFFTEGEDQLIWRATQEHGLN 228

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
            +V+ +L   I  T SEI+ER  VL E+N +   SS  +E     + +SL K++ + LDS
Sbjct: 229 REVVNVLCQFIDSTPSEIEERSEVLFEKNEKNSGSSDKIE-----RQLSLDKTMDAVLDS 288

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQ-RTQRKTPDQ 180
           FDNLFCRRC++FDCRLHGCSQ+L++P+EK  Y  E +E +KPC  QC+L+ R   +    
Sbjct: 289 FDNLFCRRCLVFDCRLHGCSQNLVFPTEKQPYSFEPDENKKPCGRQCYLRWRGGFQEIHD 348

Query: 181 RNKRPRSSKHEES---SAHVDAEII---QDERCLTGKLSSITSEGVSVSEMTVGLNLDAS 240
                 ++ + ES   S  VD  I+   +D     G + S+T  G S S++   ++ + S
Sbjct: 349 VGLSGCATYNMESGTVSHKVDVSIMSESEDSNREKGNIRSMTLVGTSGSKIISSVSAEES 408

Query: 241 VGP----ATNPRNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLS-----ATDVAN 300
             P     +   N +         K  +S         SP  K+QK+ +     A+++ N
Sbjct: 409 TTPPSADTSETENASSDMPPSSLRKYKISKRGPRYRERSPG-KRQKVFTSDISFASNILN 468

Query: 301 APTDTIPELCSTSTKHSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQD 360
               +IPE+  T  +        LQ ++    + S  +  G+       S I + +N+  
Sbjct: 469 KL--SIPEIRDTRLESREPGGDKLQILDESTKKTSSKDICGE-------SPITTTENM-- 528

Query: 361 TSRCRTVEVKELSTSKLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRN 420
                 +E K++S++K   +            S W  +E++LY+KGIEIFG+NSCLI+RN
Sbjct: 529 -----GIESKKVSSTKNFLEHT---------LSCWSALERDLYLKGIEIFGKNSCLIARN 588

Query: 421 LLSGLKTCMEVFNYMHNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRG 480
           LLSG+KTCMEV NYM+N GA+ ++    +  S + D  +T+ DYMEQDM  R+R+ R+RG
Sbjct: 589 LLSGMKTCMEVANYMYNNGAAMAKRP-LLNKSISGDFAETEQDYMEQDMVARTRIYRRRG 648

Query: 481 KARKLKYSWKSAGHPSFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGC 540
           + RKLKY+WKSAGHP+  KRI DGK Q   QY PC C   CGK CPC+ NGTCCEKYCGC
Sbjct: 649 RNRKLKYTWKSAGHPTVRKRIGDGK-QWYTQYNPCVCQQMCGKDCPCVENGTCCEKYCGC 708

Query: 541 SKSCKNRFRGCHCAKSQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGD 600
           SKSCKN+FRGCHCAKSQCRSRQCPCFAA RECDPDVCRNCWVSCGDG    LGEPP +GD
Sbjct: 709 SKSCKNKFRGCHCAKSQCRSRQCPCFAASRECDPDVCRNCWVSCGDGS---LGEPPARGD 768

Query: 601 G-QCGNMRLLLRQQQRILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKI 660
           G QCGNM+LLL+QQQRILL +SDVAGWGAF+KN VNKNDYLGEYTGELISH+EADKRGKI
Sbjct: 769 GYQCGNMKLLLKQQQRILLGRSDVAGWGAFIKNPVNKNDYLGEYTGELISHKEADKRGKI 828

Query: 661 YDRANSSFLFDLNDQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIE 720
           YDRANSSFLFDLNDQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGI+AKE IE
Sbjct: 829 YDRANSSFLFDLNDQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIYAKEHIE 888

Query: 721 ASEELFYDYRYGPDQAPAWARKPEGSKRDDASISQGRAKK 744
           ASEELFYDYRYGPDQAPAWAR+PEGSK+D+AS+S  RA K
Sbjct: 889 ASEELFYDYRYGPDQAPAWARRPEGSKKDEASVSHHRAHK 892

BLAST of Tan0010400 vs. ExPASy Swiss-Prot
Match: Q8S4P5 (Histone-lysine N-methyltransferase EZ2 OS=Zea mays OX=4577 GN=EZ2 PE=2 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 7.6e-215
Identity = 414/763 (54.26%), Postives = 519/763 (68.02%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MA+DQSVVGRRRIYYD  G+EALICSDS+EE+ EPE+EKH F++GED ++W   Q+HGL 
Sbjct: 165 MADDQSVVGRRRIYYDTVGNEALICSDSDEEIPEPEEEKHFFTKGEDHLIWRATQDHGLN 224

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
           ++V+ +L   IG T SEI+ER  VL E+N +   SS  +E       +SL K++ + LDS
Sbjct: 225 QEVVNVLCQFIGATPSEIEERSEVLFEKNEKHSGSSDKIE-----SRLSLDKTMDAVLDS 284

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRTQ-----RK 180
           FDNLFCRRC++FDCRLHGCSQ+L++P EK  Y  + +E +KPC + C+L+  Q     ++
Sbjct: 285 FDNLFCRRCLVFDCRLHGCSQNLVFPCEKQPYSFDPDENKKPCGHLCYLRFPQWREGFKE 344

Query: 181 TPDQRNKRPRSSKHEESSA--HVDAEII---QDERCLTGKLSSITSEGVSVSEMTVGLNL 240
             D       +   E  +A   VD  ++   +D     G + S+T  G S S++   ++ 
Sbjct: 345 MHDDGLAGGATYTMESGTASQRVDVNVMYESEDSNRQKGNIRSMTLVGTSGSKIISSVSA 404

Query: 241 DASV----GPATNPRNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSAT-----D 300
           + S        +   N +         K  +S        +SP  K+QK+ ++      +
Sbjct: 405 EESTTTPSADISETENVSSDLPPSSLRKHKISKHGPRYREHSPG-KRQKVFTSDISFEGN 464

Query: 301 VANAPTDTIPELCSTSTKHSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDN 360
           + N    +IPE+  T  +        L+ ++    + S  +  G+       S   + +N
Sbjct: 465 IMNKL--SIPEIRDTRLESRESGGDKLRILDESTKKTSRKDMCGE-------SPATTMEN 524

Query: 361 LQDTSRCRTVEVKELSTSKLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLI 420
           +   S       K  ST   L  ++S      C    W  +E++LY+KGIEIFG+NSCLI
Sbjct: 525 VGRQSN------KVSSTKNFLESTLS------C----WSALERDLYLKGIEIFGKNSCLI 584

Query: 421 SRNLLSGLKTCMEVFNYMHNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLR 480
           +RNLLSGLKTC+EV NYM+N GA+ ++    +  S + D  + + DYMEQDM  R+R+ R
Sbjct: 585 ARNLLSGLKTCIEVANYMYNNGAAMAKRP-LLNKSISGDFAENEQDYMEQDMAARTRIYR 644

Query: 481 KRGKARKLKYSWKSAGHPSFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKY 540
           +RG+ RKLKY+WKSAGHP+  KR  DGK Q   QY+PC C   CGK CPC   GTCCEKY
Sbjct: 645 RRGRNRKLKYTWKSAGHPTVRKRTDDGK-QCYTQYSPCACQQMCGKDCPCADKGTCCEKY 704

Query: 541 CGCSKSCKNRFRGCHCAKSQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPR 600
           CGCSKSCKN+FRGCHCAKSQCRSRQCPCFAA RECDPDVCRNCWVSCGDG    LGEP  
Sbjct: 705 CGCSKSCKNKFRGCHCAKSQCRSRQCPCFAASRECDPDVCRNCWVSCGDGS---LGEPLA 764

Query: 601 QGDG-QCGNMRLLLRQQQRILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKR 660
           +GDG QCGNM+LLL+QQQRILL +SDVAGWGAF+KN VNKNDYLGEYTGELISH+EADKR
Sbjct: 765 RGDGYQCGNMKLLLKQQQRILLGRSDVAGWGAFIKNPVNKNDYLGEYTGELISHKEADKR 824

Query: 661 GKIYDRANSSFLFDLNDQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKE 720
           GKIYDRANSSFLFDLNDQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGI+AKE
Sbjct: 825 GKIYDRANSSFLFDLNDQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIYAKE 884

Query: 721 RIEASEELFYDYRYGPDQAPAWARKPEGSKRDDASISQGRAKK 744
            IEASEELFYDYRYGPDQAPAWAR+PEGSK+D+AS+S  RA K
Sbjct: 885 HIEASEELFYDYRYGPDQAPAWARRPEGSKKDEASVSHRRAHK 891

BLAST of Tan0010400 vs. ExPASy Swiss-Prot
Match: Q10MI4 (Histone-lysine N-methyltransferase EZ1 OS=Oryza sativa subsp. japonica OX=39947 GN=EZ1 PE=1 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 1.7e-214
Identity = 418/755 (55.36%), Postives = 514/755 (68.08%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MA+DQS VGRRRIYYD   +EALICS+S+++V EPE+EKH F+EGED+++W   Q+HGL 
Sbjct: 171 MADDQS-VGRRRIYYDPIVNEALICSESDDDVPEPEEEKHVFTEGEDQLIWKATQDHGLS 230

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
            +VL +L   +  T SEI+ER  VL E+  +   SS     +K++  + L K++   LDS
Sbjct: 231 REVLNVLCQFVDATPSEIEERSEVLFEKYEKQSQSS-----YKTDLQLFLDKTMDVALDS 290

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRT---QRKTP 180
           FDNLFCRRC++FDCRLHGCSQ+L++PSEK  Y  E +E ++PC +QC+L+R    Q    
Sbjct: 291 FDNLFCRRCLVFDCRLHGCSQNLVFPSEKQPYGHELDENKRPCGDQCYLRRREVYQDTCN 350

Query: 181 DQRNK-RPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGV---SVSEMTVGLNLDAS 240
           D RN     +     SS  V A I+ +         +I S  +   S S++T     D S
Sbjct: 351 DDRNACTTYNMDSRSSSLKVSATILSESEDSNRDEDNIKSTSIVETSRSKITNSEYADKS 410

Query: 241 V----GPATNPRNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDT 300
           V    G A+   N +    ++   +  +S      +++SPD K+QK+ S+          
Sbjct: 411 VTPPPGDASETENVSPDMPLRTLGRRKISKHASKSNDHSPD-KRQKIYSS---------P 470

Query: 301 IPELCSTSTKHSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCR 360
            P   S   K S  +I        +    S+  A+ Q      P+   S  ++   S   
Sbjct: 471 FPFAMSVLNKQSVPEIG-------ETCPDSIESAVDQLPSLDDPNKKISTKDMCAGSTTN 530

Query: 361 TVEVKELSTSKLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGL 420
           T E      +  L  S  E        S W  +E++LY+KGIEIFG+NSCLI+RNLLSGL
Sbjct: 531 TTENTLRDNNNNLFISNKEH-----SISHWSALERDLYLKGIEIFGKNSCLIARNLLSGL 590

Query: 421 KTCMEVFNYMHNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKL 480
           KTCMEV +YM+N GA+ ++       S   D  + +  YMEQD+  R+R+ R++G+ARKL
Sbjct: 591 KTCMEVASYMYNNGAAMAKRP-LSGKSILGDFAEAEQGYMEQDLVARTRICRRKGRARKL 650

Query: 481 KYSWKSAGHPSFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCK 540
           KY+WKSAGHP+  KRI DGK Q   QY PCGC   CGK C C+ NGTCCEKYCGCSKSCK
Sbjct: 651 KYTWKSAGHPTVRKRIGDGK-QWYTQYNPCGCQQMCGKDCACVENGTCCEKYCGCSKSCK 710

Query: 541 NRFRGCHCAKSQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDG-QCG 600
           NRFRGCHCAKSQCRSRQCPCFAA RECDPDVCRNCWVSCGDG    LGEP  +GDG QCG
Sbjct: 711 NRFRGCHCAKSQCRSRQCPCFAASRECDPDVCRNCWVSCGDGS---LGEPLARGDGYQCG 770

Query: 601 NMRLLLRQQQRILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRAN 660
           NM+LLL+QQQRILL KSDVAGWGAF+KN VN+NDYLGEYTGELISHREADKRGKIYDRAN
Sbjct: 771 NMKLLLKQQQRILLGKSDVAGWGAFIKNPVNRNDYLGEYTGELISHREADKRGKIYDRAN 830

Query: 661 SSFLFDLNDQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEEL 720
           SSFLFDLN+QYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGI+AK+RIEASEEL
Sbjct: 831 SSFLFDLNEQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIYAKDRIEASEEL 890

Query: 721 FYDYRYGPDQAPAWARKPEGSKRDDASISQGRAKK 744
           FYDYRYGPDQAPAWAR+PEGSK+D+AS+S  RA K
Sbjct: 891 FYDYRYGPDQAPAWARRPEGSKKDEASVSHHRAHK 892

BLAST of Tan0010400 vs. ExPASy Swiss-Prot
Match: Q84UI6 (Histone-lysine N-methyltransferase EZ1 OS=Oryza sativa subsp. indica OX=39946 GN=EZ1 PE=2 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 1.5e-210
Identity = 413/755 (54.70%), Postives = 512/755 (67.81%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MA+DQ +V RRRIYYD   +EALICS+S+++V EPE+EKH F+EGED+++W   Q+HGL 
Sbjct: 171 MADDQ-LVCRRRIYYDPIVNEALICSESDDDVPEPEEEKHVFTEGEDQLIWKATQDHGLS 230

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
            +VL +L   +  T SEI+ER  VL E+  +   SS     ++++  + L K++   LDS
Sbjct: 231 REVLNVLCQFVDATPSEIEERSEVLFEKYEKQSQSS-----YETDFQLFLGKTMDVALDS 290

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRT---QRKTP 180
           FDNLFCRRC++FDCRLHGCSQ+L++PSEK  Y    +E ++PC +Q +L+R    Q    
Sbjct: 291 FDNLFCRRCLVFDCRLHGCSQNLVFPSEKQPYGHGLDENKRPCGDQRYLRRREVYQDTCN 350

Query: 181 DQRNK-RPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGV---SVSEMTVGLNLDAS 240
           D RN     ++    SS  V A I+ +         +I S  +   S S++T     D S
Sbjct: 351 DDRNACTTYNTDSRSSSLKVSATILSESEDSNRDEDNIKSTSIVETSRSKITNSEYADKS 410

Query: 241 V----GPATNPRNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDT 300
           V    G A+   N +    ++   +  +S      +++SPD K+QK+ S+          
Sbjct: 411 VTPPPGDASETENVSPDMPLRTLGRRKISKHASKSNDHSPD-KRQKIYSS---------P 470

Query: 301 IPELCSTSTKHSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCR 360
            P   S   K S  +I        +    S+  A+ Q      P+   S  ++   S   
Sbjct: 471 FPFAMSVLNKQSVPEIG-------ETCPDSIESAVDQLPSLDDPNKKISTKDMCAGSTTN 530

Query: 361 TVEVKELSTSKLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGL 420
           T E      +  L  S  E        S W  +E++LY+KGIEIFG+NSCLI+RNLLSGL
Sbjct: 531 TTENTLRDNNNNLFISNKEH-----SISHWSALERDLYLKGIEIFGKNSCLIARNLLSGL 590

Query: 421 KTCMEVFNYMHNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKL 480
           KTCMEV +YM+N GA+ ++       S   D  + +  YMEQD+  R+R+ R++G+ARKL
Sbjct: 591 KTCMEVASYMYNNGAAMAKRP-LSGKSILGDFAEAEQGYMEQDLVARTRICRRKGRARKL 650

Query: 481 KYSWKSAGHPSFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCK 540
           KY+WKSAGHP+  KRI DGK Q   QY PCGC   CGK C C+ NGTCCEKYCGCSKSCK
Sbjct: 651 KYTWKSAGHPTVRKRIGDGK-QWYTQYNPCGCQQMCGKDCACVENGTCCEKYCGCSKSCK 710

Query: 541 NRFRGCHCAKSQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDG-QCG 600
           NRFRGCHCAKSQCRSRQCPCFAA RECDPDVCRNCWVSCGDG    LGEP  +GDG QCG
Sbjct: 711 NRFRGCHCAKSQCRSRQCPCFAASRECDPDVCRNCWVSCGDGS---LGEPLARGDGYQCG 770

Query: 601 NMRLLLRQQQRILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRAN 660
           NM+LLL+QQQRILL KSDVAGWGAF+KN VN+NDYLGEYTGELISHREADKRGKIYDRAN
Sbjct: 771 NMKLLLKQQQRILLGKSDVAGWGAFIKNPVNRNDYLGEYTGELISHREADKRGKIYDRAN 830

Query: 661 SSFLFDLNDQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEEL 720
           SSFLFDLN+QYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGI+AK+RIEASEEL
Sbjct: 831 SSFLFDLNEQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIYAKDRIEASEEL 890

Query: 721 FYDYRYGPDQAPAWARKPEGSKRDDASISQGRAKK 744
           FYDYRYGPDQAPAWAR+PEGSK+D+AS+S  RA K
Sbjct: 891 FYDYRYGPDQAPAWARRPEGSKKDEASVSHHRAHK 892

BLAST of Tan0010400 vs. NCBI nr
Match: XP_022948801.1 (histone-lysine N-methyltransferase EZA1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1332.8 bits (3448), Expect = 0.0e+00
Identity = 663/748 (88.64%), Postives = 699/748 (93.45%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MAEDQSVVGRRRIYYDQHGSEALICSDSEEE+AEPEDEKHEFSEGEDRVL IIIQEHG+G
Sbjct: 140 MAEDQSVVGRRRIYYDQHGSEALICSDSEEELAEPEDEKHEFSEGEDRVLQIIIQEHGVG 199

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
           +DVLQILSHSIGCTTSEIQER+NVL+ERN +TDSSSKALE+ KS KGISLYKSLSSTLDS
Sbjct: 200 QDVLQILSHSIGCTTSEIQERFNVLEERNCKTDSSSKALEELKSGKGISLYKSLSSTLDS 259

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRTQRKTPDQR 180
           FDNLFCRRCMLFDCRLHGCSQSL YP EKLLYWPEHEEERKPCSNQCFL++TQ KTP++ 
Sbjct: 260 FDNLFCRRCMLFDCRLHGCSQSLTYPIEKLLYWPEHEEERKPCSNQCFLKQTQGKTPERS 319

Query: 181 NKRPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGVSVSEMTVGLNLDASVGPATNP 240
           NKRPRSSK EESSAHVDA+I+Q+ERCL  KL SIT E +SV+E T+GLN DASVG A +P
Sbjct: 320 NKRPRSSKPEESSAHVDADIVQNERCLAEKLISITPESISVTETTIGLNSDASVGTAISP 379

Query: 241 RNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDTIPELCS-TSTK 300
             G KQR V+HQNKVS SNDPELISNNS DCKKQK+LSATDV NAPTDTIP LC+ TST+
Sbjct: 380 ETGTKQRAVEHQNKVSASNDPELISNNSSDCKKQKMLSATDVVNAPTDTIPGLCNITSTR 439

Query: 301 HSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCRTVEVKELSTS 360
           ++HEDI GLQKIELQRN+ISLS A GQA+EK+PPSDI SCDNL DTSR  TVEVKELSTS
Sbjct: 440 YNHEDIHGLQKIELQRNDISLSGATGQAKEKVPPSDITSCDNL-DTSRYSTVEVKELSTS 499

Query: 361 KLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 420
           KL  ++V+EQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM
Sbjct: 500 KLSVEAVNEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 559

Query: 421 HNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 480
           HNGGASTSQ SN   +SNADDNGK DIDY EQDMRTRSRLLRKRGKARKLKYSWKSAGHP
Sbjct: 560 HNGGASTSQRSNSTLNSNADDNGKIDIDYKEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 619

Query: 481 SFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 540
           SFWKRIADGKNQSCKQY PCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK
Sbjct: 620 SFWKRIADGKNQSCKQYAPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 679

Query: 541 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDGQCGNMRLLLRQQQR 600
           SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDG    LGEPPRQGDGQCGNMRLLLRQQQR
Sbjct: 680 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGS---LGEPPRQGDGQCGNMRLLLRQQQR 739

Query: 601 ILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 660
           ILL+KSDVAGWGAFLKN+VNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY
Sbjct: 740 ILLSKSDVAGWGAFLKNSVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 799

Query: 661 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEELFYDYRYGPDQA 720
           VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKE I+ASEELFYDYRYGPDQA
Sbjct: 800 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKEHIDASEELFYDYRYGPDQA 859

Query: 721 PAWARKPEGSKRDDASISQGRAKKHQSH 748
           PAWAR+PEGSKRDD S+SQGRAKKHQSH
Sbjct: 860 PAWARRPEGSKRDDGSVSQGRAKKHQSH 883

BLAST of Tan0010400 vs. NCBI nr
Match: XP_022948803.1 (histone-lysine N-methyltransferase EZA1 isoform X3 [Cucurbita moschata])

HSP 1 Score: 1332.8 bits (3448), Expect = 0.0e+00
Identity = 663/748 (88.64%), Postives = 699/748 (93.45%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MAEDQSVVGRRRIYYDQHGSEALICSDSEEE+AEPEDEKHEFSEGEDRVL IIIQEHG+G
Sbjct: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEELAEPEDEKHEFSEGEDRVLQIIIQEHGVG 60

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
           +DVLQILSHSIGCTTSEIQER+NVL+ERN +TDSSSKALE+ KS KGISLYKSLSSTLDS
Sbjct: 61  QDVLQILSHSIGCTTSEIQERFNVLEERNCKTDSSSKALEELKSGKGISLYKSLSSTLDS 120

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRTQRKTPDQR 180
           FDNLFCRRCMLFDCRLHGCSQSL YP EKLLYWPEHEEERKPCSNQCFL++TQ KTP++ 
Sbjct: 121 FDNLFCRRCMLFDCRLHGCSQSLTYPIEKLLYWPEHEEERKPCSNQCFLKQTQGKTPERS 180

Query: 181 NKRPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGVSVSEMTVGLNLDASVGPATNP 240
           NKRPRSSK EESSAHVDA+I+Q+ERCL  KL SIT E +SV+E T+GLN DASVG A +P
Sbjct: 181 NKRPRSSKPEESSAHVDADIVQNERCLAEKLISITPESISVTETTIGLNSDASVGTAISP 240

Query: 241 RNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDTIPELCS-TSTK 300
             G KQR V+HQNKVS SNDPELISNNS DCKKQK+LSATDV NAPTDTIP LC+ TST+
Sbjct: 241 ETGTKQRAVEHQNKVSASNDPELISNNSSDCKKQKMLSATDVVNAPTDTIPGLCNITSTR 300

Query: 301 HSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCRTVEVKELSTS 360
           ++HEDI GLQKIELQRN+ISLS A GQA+EK+PPSDI SCDNL DTSR  TVEVKELSTS
Sbjct: 301 YNHEDIHGLQKIELQRNDISLSGATGQAKEKVPPSDITSCDNL-DTSRYSTVEVKELSTS 360

Query: 361 KLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 420
           KL  ++V+EQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM
Sbjct: 361 KLSVEAVNEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 420

Query: 421 HNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 480
           HNGGASTSQ SN   +SNADDNGK DIDY EQDMRTRSRLLRKRGKARKLKYSWKSAGHP
Sbjct: 421 HNGGASTSQRSNSTLNSNADDNGKIDIDYKEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 480

Query: 481 SFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 540
           SFWKRIADGKNQSCKQY PCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK
Sbjct: 481 SFWKRIADGKNQSCKQYAPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 540

Query: 541 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDGQCGNMRLLLRQQQR 600
           SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDG    LGEPPRQGDGQCGNMRLLLRQQQR
Sbjct: 541 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGS---LGEPPRQGDGQCGNMRLLLRQQQR 600

Query: 601 ILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 660
           ILL+KSDVAGWGAFLKN+VNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY
Sbjct: 601 ILLSKSDVAGWGAFLKNSVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 660

Query: 661 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEELFYDYRYGPDQA 720
           VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKE I+ASEELFYDYRYGPDQA
Sbjct: 661 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKEHIDASEELFYDYRYGPDQA 720

Query: 721 PAWARKPEGSKRDDASISQGRAKKHQSH 748
           PAWAR+PEGSKRDD S+SQGRAKKHQSH
Sbjct: 721 PAWARRPEGSKRDDGSVSQGRAKKHQSH 744

BLAST of Tan0010400 vs. NCBI nr
Match: XP_022998060.1 (histone-lysine N-methyltransferase EZA1 isoform X3 [Cucurbita maxima])

HSP 1 Score: 1328.5 bits (3437), Expect = 0.0e+00
Identity = 662/748 (88.50%), Postives = 696/748 (93.05%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MAEDQSVVGRRRIYYDQHGSEALICSDSEEE+AEPEDEKHEFSEGEDRVL IIIQEHG+G
Sbjct: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEELAEPEDEKHEFSEGEDRVLQIIIQEHGVG 60

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
           EDVLQILSHSIGCTTSEIQER+NVLKERN +TDSSSKALE+ KSN GISLYKSLSSTLDS
Sbjct: 61  EDVLQILSHSIGCTTSEIQERFNVLKERNCKTDSSSKALEELKSNNGISLYKSLSSTLDS 120

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRTQRKTPDQR 180
           FDNLFCRRCMLFDCRLHGCSQSL YP EKLLYWPEHEEERKPCSNQCFL++TQ KTP++ 
Sbjct: 121 FDNLFCRRCMLFDCRLHGCSQSLTYPIEKLLYWPEHEEERKPCSNQCFLKQTQGKTPERS 180

Query: 181 NKRPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGVSVSEMTVGLNLDASVGPATNP 240
           NKRPRSSK EE SAHVDA+I+Q+ERCL  KL SIT E +SV+E T+GLN DASVG A +P
Sbjct: 181 NKRPRSSKPEELSAHVDADIVQNERCLAEKLISITPESISVTETTIGLNSDASVGTAISP 240

Query: 241 RNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDTIPELCS-TSTK 300
             G KQR V+HQNKVS SNDPELISNNS DCKKQK+LSATDV NAPTDTIP LC+ T T+
Sbjct: 241 ETGTKQRAVEHQNKVSASNDPELISNNSSDCKKQKMLSATDVVNAPTDTIPGLCNITITR 300

Query: 301 HSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCRTVEVKELSTS 360
           ++HEDI GLQKIELQRN+ISLSEA GQA+EK+PPSDI SCDNL DT R  TVEVKELSTS
Sbjct: 301 YNHEDIHGLQKIELQRNDISLSEATGQAKEKVPPSDITSCDNL-DTGRYSTVEVKELSTS 360

Query: 361 KLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 420
           KL  ++V+EQVEGTC SS WKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM
Sbjct: 361 KLSVEAVNEQVEGTCESSLWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 420

Query: 421 HNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 480
           HNGGASTSQ SN   +SNADDNGKTDIDY EQDMRTRSRLLRKRGKARKLKYSWKSAGHP
Sbjct: 421 HNGGASTSQRSNSTLNSNADDNGKTDIDYKEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 480

Query: 481 SFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 540
           SFWKRIADGKNQSCKQY PCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK
Sbjct: 481 SFWKRIADGKNQSCKQYAPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 540

Query: 541 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDGQCGNMRLLLRQQQR 600
           SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDG    LGEPPRQGDGQCGNMRLLLRQQQR
Sbjct: 541 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGS---LGEPPRQGDGQCGNMRLLLRQQQR 600

Query: 601 ILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 660
           ILL+KSDVAGWGAFLKN+VNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY
Sbjct: 601 ILLSKSDVAGWGAFLKNSVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 660

Query: 661 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEELFYDYRYGPDQA 720
           VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKE I+ASEELFYDYRYGPDQA
Sbjct: 661 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKEHIDASEELFYDYRYGPDQA 720

Query: 721 PAWARKPEGSKRDDASISQGRAKKHQSH 748
           PAWAR+PEGSKRDD S+SQGRAKKHQSH
Sbjct: 721 PAWARRPEGSKRDDGSVSQGRAKKHQSH 744

BLAST of Tan0010400 vs. NCBI nr
Match: XP_022998058.1 (histone-lysine N-methyltransferase EZA1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1328.5 bits (3437), Expect = 0.0e+00
Identity = 662/748 (88.50%), Postives = 696/748 (93.05%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MAEDQSVVGRRRIYYDQHGSEALICSDSEEE+AEPEDEKHEFSEGEDRVL IIIQEHG+G
Sbjct: 140 MAEDQSVVGRRRIYYDQHGSEALICSDSEEELAEPEDEKHEFSEGEDRVLQIIIQEHGVG 199

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
           EDVLQILSHSIGCTTSEIQER+NVLKERN +TDSSSKALE+ KSN GISLYKSLSSTLDS
Sbjct: 200 EDVLQILSHSIGCTTSEIQERFNVLKERNCKTDSSSKALEELKSNNGISLYKSLSSTLDS 259

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRTQRKTPDQR 180
           FDNLFCRRCMLFDCRLHGCSQSL YP EKLLYWPEHEEERKPCSNQCFL++TQ KTP++ 
Sbjct: 260 FDNLFCRRCMLFDCRLHGCSQSLTYPIEKLLYWPEHEEERKPCSNQCFLKQTQGKTPERS 319

Query: 181 NKRPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGVSVSEMTVGLNLDASVGPATNP 240
           NKRPRSSK EE SAHVDA+I+Q+ERCL  KL SIT E +SV+E T+GLN DASVG A +P
Sbjct: 320 NKRPRSSKPEELSAHVDADIVQNERCLAEKLISITPESISVTETTIGLNSDASVGTAISP 379

Query: 241 RNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDTIPELCS-TSTK 300
             G KQR V+HQNKVS SNDPELISNNS DCKKQK+LSATDV NAPTDTIP LC+ T T+
Sbjct: 380 ETGTKQRAVEHQNKVSASNDPELISNNSSDCKKQKMLSATDVVNAPTDTIPGLCNITITR 439

Query: 301 HSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCRTVEVKELSTS 360
           ++HEDI GLQKIELQRN+ISLSEA GQA+EK+PPSDI SCDNL DT R  TVEVKELSTS
Sbjct: 440 YNHEDIHGLQKIELQRNDISLSEATGQAKEKVPPSDITSCDNL-DTGRYSTVEVKELSTS 499

Query: 361 KLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 420
           KL  ++V+EQVEGTC SS WKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM
Sbjct: 500 KLSVEAVNEQVEGTCESSLWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 559

Query: 421 HNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 480
           HNGGASTSQ SN   +SNADDNGKTDIDY EQDMRTRSRLLRKRGKARKLKYSWKSAGHP
Sbjct: 560 HNGGASTSQRSNSTLNSNADDNGKTDIDYKEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 619

Query: 481 SFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 540
           SFWKRIADGKNQSCKQY PCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK
Sbjct: 620 SFWKRIADGKNQSCKQYAPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 679

Query: 541 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDGQCGNMRLLLRQQQR 600
           SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDG    LGEPPRQGDGQCGNMRLLLRQQQR
Sbjct: 680 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGS---LGEPPRQGDGQCGNMRLLLRQQQR 739

Query: 601 ILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 660
           ILL+KSDVAGWGAFLKN+VNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY
Sbjct: 740 ILLSKSDVAGWGAFLKNSVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 799

Query: 661 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEELFYDYRYGPDQA 720
           VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKE I+ASEELFYDYRYGPDQA
Sbjct: 800 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKEHIDASEELFYDYRYGPDQA 859

Query: 721 PAWARKPEGSKRDDASISQGRAKKHQSH 748
           PAWAR+PEGSKRDD S+SQGRAKKHQSH
Sbjct: 860 PAWARRPEGSKRDDGSVSQGRAKKHQSH 883

BLAST of Tan0010400 vs. NCBI nr
Match: XP_023524793.1 (histone-lysine N-methyltransferase EZA1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1326.2 bits (3431), Expect = 0.0e+00
Identity = 660/748 (88.24%), Postives = 697/748 (93.18%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MAEDQSVVGRRRIYYDQHGSEALICSDSEEE+AEPEDEKHEFSEGEDRVL IIIQEHG+G
Sbjct: 140 MAEDQSVVGRRRIYYDQHGSEALICSDSEEELAEPEDEKHEFSEGEDRVLQIIIQEHGVG 199

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
           EDVLQILSHSIGCTTSEIQER+NVL+ERN +TDSSSKALE+ KS+KGISLYKSLSSTLDS
Sbjct: 200 EDVLQILSHSIGCTTSEIQERFNVLEERNCKTDSSSKALEELKSSKGISLYKSLSSTLDS 259

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRTQRKTPDQR 180
           FDNLFCRRCMLFDCRLHGCSQSL YP EKLLYWPEHEEERKPCSNQCFL++TQ KTP++ 
Sbjct: 260 FDNLFCRRCMLFDCRLHGCSQSLTYPIEKLLYWPEHEEERKPCSNQCFLKQTQGKTPERS 319

Query: 181 NKRPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGVSVSEMTVGLNLDASVGPATNP 240
           NKRPRSS+ EESSAHVDA+I+Q+ERCL  KL SIT E +SV+E T+GLN DASVG A +P
Sbjct: 320 NKRPRSSRPEESSAHVDADIVQNERCLAEKLISITPESISVTETTIGLNSDASVGAAISP 379

Query: 241 RNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDTIPELCS-TSTK 300
             G KQR V+HQNKVS SNDPELISNNS DCKKQK+LSATDV NAP DTIP LC+ TST+
Sbjct: 380 ETGTKQRAVEHQNKVSASNDPELISNNSSDCKKQKMLSATDVVNAPNDTIPGLCNITSTR 439

Query: 301 HSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCRTVEVKELSTS 360
           + HEDI GL KIELQRN+ISLSEA GQA+EK+PPSDI+SCDNL DTS   TVEVKELST 
Sbjct: 440 YKHEDIHGLPKIELQRNDISLSEATGQAKEKVPPSDISSCDNL-DTSSYSTVEVKELSTL 499

Query: 361 KLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 420
           KL  ++V+EQVEGTC SSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM
Sbjct: 500 KLSVEAVNEQVEGTCESSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 559

Query: 421 HNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 480
           HNGGASTSQ SN   +SNADDNGKTDIDY EQDMRTRSRLLRKRGKARKLKYSWKSAGHP
Sbjct: 560 HNGGASTSQRSNSTLNSNADDNGKTDIDYKEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 619

Query: 481 SFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 540
           SFWKRIADGKNQSCKQY PCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK
Sbjct: 620 SFWKRIADGKNQSCKQYAPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 679

Query: 541 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDGQCGNMRLLLRQQQR 600
           SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDG    LGEPPRQGDGQCGNMRLLLRQQQR
Sbjct: 680 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGS---LGEPPRQGDGQCGNMRLLLRQQQR 739

Query: 601 ILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 660
           ILL+KSDVAGWGAFLKN+VNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY
Sbjct: 740 ILLSKSDVAGWGAFLKNSVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 799

Query: 661 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEELFYDYRYGPDQA 720
           VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKE I+ASEELFYDYRYGPDQA
Sbjct: 800 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKEHIDASEELFYDYRYGPDQA 859

Query: 721 PAWARKPEGSKRDDASISQGRAKKHQSH 748
           PAWAR+PEGSKRDD S+SQGRAKKHQSH
Sbjct: 860 PAWARRPEGSKRDDGSVSQGRAKKHQSH 883

BLAST of Tan0010400 vs. ExPASy TrEMBL
Match: A0A6J1GA75 (histone-lysine N-methyltransferase EZA1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452363 PE=4 SV=1)

HSP 1 Score: 1332.8 bits (3448), Expect = 0.0e+00
Identity = 663/748 (88.64%), Postives = 699/748 (93.45%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MAEDQSVVGRRRIYYDQHGSEALICSDSEEE+AEPEDEKHEFSEGEDRVL IIIQEHG+G
Sbjct: 140 MAEDQSVVGRRRIYYDQHGSEALICSDSEEELAEPEDEKHEFSEGEDRVLQIIIQEHGVG 199

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
           +DVLQILSHSIGCTTSEIQER+NVL+ERN +TDSSSKALE+ KS KGISLYKSLSSTLDS
Sbjct: 200 QDVLQILSHSIGCTTSEIQERFNVLEERNCKTDSSSKALEELKSGKGISLYKSLSSTLDS 259

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRTQRKTPDQR 180
           FDNLFCRRCMLFDCRLHGCSQSL YP EKLLYWPEHEEERKPCSNQCFL++TQ KTP++ 
Sbjct: 260 FDNLFCRRCMLFDCRLHGCSQSLTYPIEKLLYWPEHEEERKPCSNQCFLKQTQGKTPERS 319

Query: 181 NKRPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGVSVSEMTVGLNLDASVGPATNP 240
           NKRPRSSK EESSAHVDA+I+Q+ERCL  KL SIT E +SV+E T+GLN DASVG A +P
Sbjct: 320 NKRPRSSKPEESSAHVDADIVQNERCLAEKLISITPESISVTETTIGLNSDASVGTAISP 379

Query: 241 RNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDTIPELCS-TSTK 300
             G KQR V+HQNKVS SNDPELISNNS DCKKQK+LSATDV NAPTDTIP LC+ TST+
Sbjct: 380 ETGTKQRAVEHQNKVSASNDPELISNNSSDCKKQKMLSATDVVNAPTDTIPGLCNITSTR 439

Query: 301 HSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCRTVEVKELSTS 360
           ++HEDI GLQKIELQRN+ISLS A GQA+EK+PPSDI SCDNL DTSR  TVEVKELSTS
Sbjct: 440 YNHEDIHGLQKIELQRNDISLSGATGQAKEKVPPSDITSCDNL-DTSRYSTVEVKELSTS 499

Query: 361 KLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 420
           KL  ++V+EQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM
Sbjct: 500 KLSVEAVNEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 559

Query: 421 HNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 480
           HNGGASTSQ SN   +SNADDNGK DIDY EQDMRTRSRLLRKRGKARKLKYSWKSAGHP
Sbjct: 560 HNGGASTSQRSNSTLNSNADDNGKIDIDYKEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 619

Query: 481 SFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 540
           SFWKRIADGKNQSCKQY PCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK
Sbjct: 620 SFWKRIADGKNQSCKQYAPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 679

Query: 541 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDGQCGNMRLLLRQQQR 600
           SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDG    LGEPPRQGDGQCGNMRLLLRQQQR
Sbjct: 680 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGS---LGEPPRQGDGQCGNMRLLLRQQQR 739

Query: 601 ILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 660
           ILL+KSDVAGWGAFLKN+VNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY
Sbjct: 740 ILLSKSDVAGWGAFLKNSVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 799

Query: 661 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEELFYDYRYGPDQA 720
           VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKE I+ASEELFYDYRYGPDQA
Sbjct: 800 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKEHIDASEELFYDYRYGPDQA 859

Query: 721 PAWARKPEGSKRDDASISQGRAKKHQSH 748
           PAWAR+PEGSKRDD S+SQGRAKKHQSH
Sbjct: 860 PAWARRPEGSKRDDGSVSQGRAKKHQSH 883

BLAST of Tan0010400 vs. ExPASy TrEMBL
Match: A0A6J1GAC2 (histone-lysine N-methyltransferase EZA1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111452363 PE=4 SV=1)

HSP 1 Score: 1332.8 bits (3448), Expect = 0.0e+00
Identity = 663/748 (88.64%), Postives = 699/748 (93.45%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MAEDQSVVGRRRIYYDQHGSEALICSDSEEE+AEPEDEKHEFSEGEDRVL IIIQEHG+G
Sbjct: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEELAEPEDEKHEFSEGEDRVLQIIIQEHGVG 60

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
           +DVLQILSHSIGCTTSEIQER+NVL+ERN +TDSSSKALE+ KS KGISLYKSLSSTLDS
Sbjct: 61  QDVLQILSHSIGCTTSEIQERFNVLEERNCKTDSSSKALEELKSGKGISLYKSLSSTLDS 120

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRTQRKTPDQR 180
           FDNLFCRRCMLFDCRLHGCSQSL YP EKLLYWPEHEEERKPCSNQCFL++TQ KTP++ 
Sbjct: 121 FDNLFCRRCMLFDCRLHGCSQSLTYPIEKLLYWPEHEEERKPCSNQCFLKQTQGKTPERS 180

Query: 181 NKRPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGVSVSEMTVGLNLDASVGPATNP 240
           NKRPRSSK EESSAHVDA+I+Q+ERCL  KL SIT E +SV+E T+GLN DASVG A +P
Sbjct: 181 NKRPRSSKPEESSAHVDADIVQNERCLAEKLISITPESISVTETTIGLNSDASVGTAISP 240

Query: 241 RNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDTIPELCS-TSTK 300
             G KQR V+HQNKVS SNDPELISNNS DCKKQK+LSATDV NAPTDTIP LC+ TST+
Sbjct: 241 ETGTKQRAVEHQNKVSASNDPELISNNSSDCKKQKMLSATDVVNAPTDTIPGLCNITSTR 300

Query: 301 HSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCRTVEVKELSTS 360
           ++HEDI GLQKIELQRN+ISLS A GQA+EK+PPSDI SCDNL DTSR  TVEVKELSTS
Sbjct: 301 YNHEDIHGLQKIELQRNDISLSGATGQAKEKVPPSDITSCDNL-DTSRYSTVEVKELSTS 360

Query: 361 KLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 420
           KL  ++V+EQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM
Sbjct: 361 KLSVEAVNEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 420

Query: 421 HNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 480
           HNGGASTSQ SN   +SNADDNGK DIDY EQDMRTRSRLLRKRGKARKLKYSWKSAGHP
Sbjct: 421 HNGGASTSQRSNSTLNSNADDNGKIDIDYKEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 480

Query: 481 SFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 540
           SFWKRIADGKNQSCKQY PCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK
Sbjct: 481 SFWKRIADGKNQSCKQYAPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 540

Query: 541 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDGQCGNMRLLLRQQQR 600
           SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDG    LGEPPRQGDGQCGNMRLLLRQQQR
Sbjct: 541 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGS---LGEPPRQGDGQCGNMRLLLRQQQR 600

Query: 601 ILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 660
           ILL+KSDVAGWGAFLKN+VNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY
Sbjct: 601 ILLSKSDVAGWGAFLKNSVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 660

Query: 661 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEELFYDYRYGPDQA 720
           VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKE I+ASEELFYDYRYGPDQA
Sbjct: 661 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKEHIDASEELFYDYRYGPDQA 720

Query: 721 PAWARKPEGSKRDDASISQGRAKKHQSH 748
           PAWAR+PEGSKRDD S+SQGRAKKHQSH
Sbjct: 721 PAWARRPEGSKRDDGSVSQGRAKKHQSH 744

BLAST of Tan0010400 vs. ExPASy TrEMBL
Match: A0A6J1KFQ9 (histone-lysine N-methyltransferase EZA1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492820 PE=4 SV=1)

HSP 1 Score: 1328.5 bits (3437), Expect = 0.0e+00
Identity = 662/748 (88.50%), Postives = 696/748 (93.05%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MAEDQSVVGRRRIYYDQHGSEALICSDSEEE+AEPEDEKHEFSEGEDRVL IIIQEHG+G
Sbjct: 140 MAEDQSVVGRRRIYYDQHGSEALICSDSEEELAEPEDEKHEFSEGEDRVLQIIIQEHGVG 199

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
           EDVLQILSHSIGCTTSEIQER+NVLKERN +TDSSSKALE+ KSN GISLYKSLSSTLDS
Sbjct: 200 EDVLQILSHSIGCTTSEIQERFNVLKERNCKTDSSSKALEELKSNNGISLYKSLSSTLDS 259

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRTQRKTPDQR 180
           FDNLFCRRCMLFDCRLHGCSQSL YP EKLLYWPEHEEERKPCSNQCFL++TQ KTP++ 
Sbjct: 260 FDNLFCRRCMLFDCRLHGCSQSLTYPIEKLLYWPEHEEERKPCSNQCFLKQTQGKTPERS 319

Query: 181 NKRPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGVSVSEMTVGLNLDASVGPATNP 240
           NKRPRSSK EE SAHVDA+I+Q+ERCL  KL SIT E +SV+E T+GLN DASVG A +P
Sbjct: 320 NKRPRSSKPEELSAHVDADIVQNERCLAEKLISITPESISVTETTIGLNSDASVGTAISP 379

Query: 241 RNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDTIPELCS-TSTK 300
             G KQR V+HQNKVS SNDPELISNNS DCKKQK+LSATDV NAPTDTIP LC+ T T+
Sbjct: 380 ETGTKQRAVEHQNKVSASNDPELISNNSSDCKKQKMLSATDVVNAPTDTIPGLCNITITR 439

Query: 301 HSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCRTVEVKELSTS 360
           ++HEDI GLQKIELQRN+ISLSEA GQA+EK+PPSDI SCDNL DT R  TVEVKELSTS
Sbjct: 440 YNHEDIHGLQKIELQRNDISLSEATGQAKEKVPPSDITSCDNL-DTGRYSTVEVKELSTS 499

Query: 361 KLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 420
           KL  ++V+EQVEGTC SS WKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM
Sbjct: 500 KLSVEAVNEQVEGTCESSLWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 559

Query: 421 HNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 480
           HNGGASTSQ SN   +SNADDNGKTDIDY EQDMRTRSRLLRKRGKARKLKYSWKSAGHP
Sbjct: 560 HNGGASTSQRSNSTLNSNADDNGKTDIDYKEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 619

Query: 481 SFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 540
           SFWKRIADGKNQSCKQY PCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK
Sbjct: 620 SFWKRIADGKNQSCKQYAPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 679

Query: 541 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDGQCGNMRLLLRQQQR 600
           SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDG    LGEPPRQGDGQCGNMRLLLRQQQR
Sbjct: 680 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGS---LGEPPRQGDGQCGNMRLLLRQQQR 739

Query: 601 ILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 660
           ILL+KSDVAGWGAFLKN+VNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY
Sbjct: 740 ILLSKSDVAGWGAFLKNSVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 799

Query: 661 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEELFYDYRYGPDQA 720
           VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKE I+ASEELFYDYRYGPDQA
Sbjct: 800 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKEHIDASEELFYDYRYGPDQA 859

Query: 721 PAWARKPEGSKRDDASISQGRAKKHQSH 748
           PAWAR+PEGSKRDD S+SQGRAKKHQSH
Sbjct: 860 PAWARRPEGSKRDDGSVSQGRAKKHQSH 883

BLAST of Tan0010400 vs. ExPASy TrEMBL
Match: A0A6J1KBL0 (histone-lysine N-methyltransferase EZA1 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111492820 PE=4 SV=1)

HSP 1 Score: 1328.5 bits (3437), Expect = 0.0e+00
Identity = 662/748 (88.50%), Postives = 696/748 (93.05%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MAEDQSVVGRRRIYYDQHGSEALICSDSEEE+AEPEDEKHEFSEGEDRVL IIIQEHG+G
Sbjct: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEELAEPEDEKHEFSEGEDRVLQIIIQEHGVG 60

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
           EDVLQILSHSIGCTTSEIQER+NVLKERN +TDSSSKALE+ KSN GISLYKSLSSTLDS
Sbjct: 61  EDVLQILSHSIGCTTSEIQERFNVLKERNCKTDSSSKALEELKSNNGISLYKSLSSTLDS 120

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRTQRKTPDQR 180
           FDNLFCRRCMLFDCRLHGCSQSL YP EKLLYWPEHEEERKPCSNQCFL++TQ KTP++ 
Sbjct: 121 FDNLFCRRCMLFDCRLHGCSQSLTYPIEKLLYWPEHEEERKPCSNQCFLKQTQGKTPERS 180

Query: 181 NKRPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGVSVSEMTVGLNLDASVGPATNP 240
           NKRPRSSK EE SAHVDA+I+Q+ERCL  KL SIT E +SV+E T+GLN DASVG A +P
Sbjct: 181 NKRPRSSKPEELSAHVDADIVQNERCLAEKLISITPESISVTETTIGLNSDASVGTAISP 240

Query: 241 RNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDTIPELCS-TSTK 300
             G KQR V+HQNKVS SNDPELISNNS DCKKQK+LSATDV NAPTDTIP LC+ T T+
Sbjct: 241 ETGTKQRAVEHQNKVSASNDPELISNNSSDCKKQKMLSATDVVNAPTDTIPGLCNITITR 300

Query: 301 HSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCRTVEVKELSTS 360
           ++HEDI GLQKIELQRN+ISLSEA GQA+EK+PPSDI SCDNL DT R  TVEVKELSTS
Sbjct: 301 YNHEDIHGLQKIELQRNDISLSEATGQAKEKVPPSDITSCDNL-DTGRYSTVEVKELSTS 360

Query: 361 KLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 420
           KL  ++V+EQVEGTC SS WKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM
Sbjct: 361 KLSVEAVNEQVEGTCESSLWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 420

Query: 421 HNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 480
           HNGGASTSQ SN   +SNADDNGKTDIDY EQDMRTRSRLLRKRGKARKLKYSWKSAGHP
Sbjct: 421 HNGGASTSQRSNSTLNSNADDNGKTDIDYKEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 480

Query: 481 SFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 540
           SFWKRIADGKNQSCKQY PCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK
Sbjct: 481 SFWKRIADGKNQSCKQYAPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 540

Query: 541 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDGQCGNMRLLLRQQQR 600
           SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDG    LGEPPRQGDGQCGNMRLLLRQQQR
Sbjct: 541 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGS---LGEPPRQGDGQCGNMRLLLRQQQR 600

Query: 601 ILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 660
           ILL+KSDVAGWGAFLKN+VNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY
Sbjct: 601 ILLSKSDVAGWGAFLKNSVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 660

Query: 661 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEELFYDYRYGPDQA 720
           VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKE I+ASEELFYDYRYGPDQA
Sbjct: 661 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKEHIDASEELFYDYRYGPDQA 720

Query: 721 PAWARKPEGSKRDDASISQGRAKKHQSH 748
           PAWAR+PEGSKRDD S+SQGRAKKHQSH
Sbjct: 721 PAWARRPEGSKRDDGSVSQGRAKKHQSH 744

BLAST of Tan0010400 vs. ExPASy TrEMBL
Match: A0A6J1GA85 (histone-lysine N-methyltransferase EZA1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452363 PE=4 SV=1)

HSP 1 Score: 1324.7 bits (3427), Expect = 0.0e+00
Identity = 661/748 (88.37%), Postives = 698/748 (93.32%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MAEDQSVVGRRRIYYDQHGSEALICSDSEEE+AEPEDEKHEFSEGEDRVL IIIQEHG+G
Sbjct: 140 MAEDQSVVGRRRIYYDQHGSEALICSDSEEELAEPEDEKHEFSEGEDRVLQIIIQEHGVG 199

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
           +DVLQILSHSIGCTTSEIQER+NVL+ERN +TDSSSKALE+ KS KGISLYKSLSSTLDS
Sbjct: 200 QDVLQILSHSIGCTTSEIQERFNVLEERNCKTDSSSKALEELKSGKGISLYKSLSSTLDS 259

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRTQRKTPDQR 180
           FDNLFCRRCMLFDCRLHGCSQSL YP EKLLYWPEHEEERKPCSNQCFL++TQ KTP++ 
Sbjct: 260 FDNLFCRRCMLFDCRLHGCSQSLTYPIEKLLYWPEHEEERKPCSNQCFLKQTQGKTPERS 319

Query: 181 NKRPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGVSVSEMTVGLNLDASVGPATNP 240
           NKRPRSSK EESSAHVDA+I+Q+ERCL  KL SIT E +SV+E T+GLN DASVG A +P
Sbjct: 320 NKRPRSSKPEESSAHVDADIVQNERCLAEKLISITPESISVTETTIGLNSDASVGTAISP 379

Query: 241 RNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDTIPELCS-TSTK 300
             G KQR V+HQNKVS SNDPELISNNS DCKKQK+LSATDV NAPTDTIP LC+ TST+
Sbjct: 380 ETGTKQRAVEHQNKVSASNDPELISNNSSDCKKQKMLSATDVVNAPTDTIPGLCNITSTR 439

Query: 301 HSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCRTVEVKELSTS 360
           ++HEDI GLQKIELQRN+ISLS A GQA+EK+PPSDI SCDNL DTSR  TVEVKELSTS
Sbjct: 440 YNHEDIHGLQKIELQRNDISLSGATGQAKEKVPPSDITSCDNL-DTSRYSTVEVKELSTS 499

Query: 361 KLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 420
           KL  ++V+EQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM
Sbjct: 500 KLSVEAVNEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVFNYM 559

Query: 421 HNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKLKYSWKSAGHP 480
           HNGGASTSQ SN   +SNADDNGK DIDY  +DMRTRSRLLRKRGKARKLKYSWKSAGHP
Sbjct: 560 HNGGASTSQRSNSTLNSNADDNGKIDIDY--KDMRTRSRLLRKRGKARKLKYSWKSAGHP 619

Query: 481 SFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 540
           SFWKRIADGKNQSCKQY PCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK
Sbjct: 620 SFWKRIADGKNQSCKQYAPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCHCAK 679

Query: 541 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDGQCGNMRLLLRQQQR 600
           SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDG    LGEPPRQGDGQCGNMRLLLRQQQR
Sbjct: 680 SQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGS---LGEPPRQGDGQCGNMRLLLRQQQR 739

Query: 601 ILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 660
           ILL+KSDVAGWGAFLKN+VNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY
Sbjct: 740 ILLSKSDVAGWGAFLKNSVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQY 799

Query: 661 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEELFYDYRYGPDQA 720
           VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKE I+ASEELFYDYRYGPDQA
Sbjct: 800 VLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKEHIDASEELFYDYRYGPDQA 859

Query: 721 PAWARKPEGSKRDDASISQGRAKKHQSH 748
           PAWAR+PEGSKRDD S+SQGRAKKHQSH
Sbjct: 860 PAWARRPEGSKRDDGSVSQGRAKKHQSH 881

BLAST of Tan0010400 vs. TAIR 10
Match: AT4G02020.1 (SET domain-containing protein )

HSP 1 Score: 791.6 bits (2043), Expect = 5.6e-229
Identity = 438/751 (58.32%), Postives = 538/751 (71.64%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           MAEDQSVVGRR+IYY+QHG E LICSDSEEE  EPE+EK EFSEGED ++W+I QE+G+G
Sbjct: 145 MAEDQSVVGRRQIYYEQHGGETLICSDSEEE-PEPEEEKREFSEGEDSIIWLIGQEYGMG 204

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
           E+V   L   +    S+I ERYN LK ++ + ++   +   FK   GISL K L + LDS
Sbjct: 205 EEVQDALCQLLSVDASDILERYNELKLKD-KQNTEEFSNSGFK--LGISLEKGLGAALDS 264

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQ-RTQRKTPDQ 180
           FDNLFCRRC++FDCRLHGCSQ LI  SEK  YW ++E +RKPCS  C+LQ +  R+ P+ 
Sbjct: 265 FDNLFCRRCLVFDCRLHGCSQPLISASEKQPYWSDYEGDRKPCSKHCYLQLKAVREVPET 324

Query: 181 RNKRPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGVS--VSEMTVGL-NLDASVGP 240
            +    +SK EE ++  +       + ++  +    + GVS  V +  +G+ N+D+S G 
Sbjct: 325 CSN--FASKAEEKASEEEC-----SKAVSSDVPHAAASGVSLQVEKTDIGIKNVDSSSGV 384

Query: 241 ATNPRNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDTIPELCST 300
                   ++  ++ + +V +  D   + N S   KKQK  +A+D   +  +++P L   
Sbjct: 385 -------EQEHGIRGKREVPILKDSNDLPNLSN--KKQK-TAASDTKMSFVNSVPSL-DQ 444

Query: 301 STKHSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCRTVEVKEL 360
           +   +  D  G    ++ R+  + ++ +G   E IP + +        +S C+       
Sbjct: 445 ALDSTKGDQGGTTDNKVNRDSEADAKEVG---EPIPDNSVHD----GGSSICQPHH---- 504

Query: 361 STSKLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTCMEVF 420
             S   A  ++E  E +  S+EW  +EK+LY+KG+EIFGRNSCLI+RNLLSGLKTC++V 
Sbjct: 505 -GSGNGAIIIAEMSETSRPSTEWNPIEKDLYLKGVEIFGRNSCLIARNLLSGLKTCLDVS 564

Query: 421 NYMHNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKLKYSWKSA 480
           NYM     S  + S+  P+   DD G+TD      ++  R+RL R++GK RKLKYS KSA
Sbjct: 565 NYMRENEVSVFRRSS-TPNLLLDD-GRTDPGNDNDEVPPRTRLFRRKGKTRKLKYSTKSA 624

Query: 481 GHPSFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRFRGCH 540
           GHPS WKRIA GKNQSCKQYTPCGCLS CGK CPCL N TCCEKYCGCSKSCKNRFRGCH
Sbjct: 625 GHPSVWKRIAGGKNQSCKQYTPCGCLSMCGKDCPCLTNETCCEKYCGCSKSCKNRFRGCH 684

Query: 541 CAKSQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDGQCGNMRLLLRQ 600
           CAKSQCRSRQCPCFAAGRECDPDVCRNCWVSCGDG    LGE PR+G+GQCGNMRLLLRQ
Sbjct: 685 CAKSQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGS---LGEAPRRGEGQCGNMRLLLRQ 744

Query: 601 QQRILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLN 660
           QQRILL KSDVAGWGAFLKN+V+KN+YLGEYTGELISH EADKRGKIYDRANSSFLFDLN
Sbjct: 745 QQRILLGKSDVAGWGAFLKNSVSKNEYLGEYTGELISHHEADKRGKIYDRANSSFLFDLN 804

Query: 661 DQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEELFYDYRYGP 720
           DQYVLDA RKGDKLKFANHS+ PNCYAKVM VAGDHRVGIFA ERIEASEELFYDYRYGP
Sbjct: 805 DQYVLDAQRKGDKLKFANHSAKPNCYAKVMFVAGDHRVGIFANERIEASEELFYDYRYGP 856

Query: 721 DQAPAWARKPEGSKRDDASISQGRAKKHQSH 748
           DQAP WARKPEGSK+DD++I+  RA+KHQSH
Sbjct: 865 DQAPVWARKPEGSKKDDSAITHRRARKHQSH 856

BLAST of Tan0010400 vs. TAIR 10
Match: AT2G23380.1 (SET domain-containing protein )

HSP 1 Score: 631.7 bits (1628), Expect = 7.4e-181
Identity = 368/764 (48.17%), Postives = 475/764 (62.17%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPEDEKHEFSEGEDRVLWIIIQEHGLG 60
           M EDQSVVGRRRIYYDQ G EALICSDSEEE  + E+EK +F E ED ++ + +++ GL 
Sbjct: 157 MTEDQSVVGRRRIYYDQTGGEALICSDSEEEAIDDEEEKRDFLEPEDYIIRMTLEQLGLS 216

Query: 61  EDVLQILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKSLSSTLDS 120
           + VL  L+  +  +TSEI+ R+ VL +    ++S     E    N      K +   LDS
Sbjct: 217 DSVLAELASFLSRSTSEIKARHGVLMKEKEVSESGDNQAESSLLN------KDMEGALDS 276

Query: 121 FDNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRTQR-KTPDQ 180
           FDNLFCRRC++FDCRLHGCSQ LI+P+EK   W    +E   C   C+    +  + P  
Sbjct: 277 FDNLFCRRCLVFDCRLHGCSQDLIFPAEKPAPWCPPVDENLTCGANCYKTLLKSGRFPGY 336

Query: 181 RNKRPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGVSVSEMTVGLNLDASVG--PA 240
                ++    + +           +    K  +  SE  S +E       D+  G    
Sbjct: 337 GTIEGKTGTSSDGAGTKTTPTKFSSKLNGRKPKTFPSESASSNEKCALETSDSENGLQQD 396

Query: 241 TNPRNGAKQRDVKHQ-NKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDTIPELCST 300
           TN    +    VK    +V    +   ++   P  K QK    T+ +++ +        +
Sbjct: 397 TNSDKVSSSPKVKGSGRRVGRKRNKNRVAERVPR-KTQKRQKKTEASDSDSIASGSCSPS 456

Query: 301 STKH-SHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCRTVEV-- 360
             KH  +ED     +  ++      S   G++ +   P+++++     D   C++ EV  
Sbjct: 457 DAKHKDNEDATSSSQKHVK------SGNSGKSRKNGTPAEVSNNSVKDDVPVCQSNEVAS 516

Query: 361 --------KELSTSKLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNL 420
                   + L   + + ++VS     T  +  W+ +EK L+ KG+EIFG NSCLI+RNL
Sbjct: 517 ELDAPGSDESLRKEEFMGETVSRGRLAT--NKLWRPLEKSLFDKGVEIFGMNSCLIARNL 576

Query: 421 LSGLKTCMEVFNYMHNGGASTSQSSNRMPSSNADDNGKTDI--DYMEQDMRTRSRLLRKR 480
           LSG K+C EVF YM       S         N D + K DI  + +   +R RSR LR+R
Sbjct: 577 LSGFKSCWEVFQYMTCSENKASFFGG--DGLNPDGSSKFDINGNMVNNQVRRRSRFLRRR 636

Query: 481 GKARKLKYSWKSAGHPSFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCG 540
           GK R+LKY+WKSA + S  KRI + K+Q C+Q+ PC C  +CGK+CPCL NGTCCEKYCG
Sbjct: 637 GKVRRLKYTWKSAAYHSIRKRITEKKDQPCRQFNPCNCKIACGKECPCLLNGTCCEKYCG 696

Query: 541 CSKSCKNRFRGCHCAKSQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQG 600
           C KSCKNRFRGCHCAKSQCRSRQCPCFAA RECDPDVCRNCWV    G DG LG P ++G
Sbjct: 697 CPKSCKNRFRGCHCAKSQCRSRQCPCFAADRECDPDVCRNCWVI---GGDGSLGVPSQRG 756

Query: 601 DG-QCGNMRLLLRQQQRILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGK 660
           D  +C NM+LLL+QQQR+LL  SDV+GWGAFLKN+V+K++YLGEYTGELISH+EADKRGK
Sbjct: 757 DNYECRNMKLLLKQQQRVLLGISDVSGWGAFLKNSVSKHEYLGEYTGELISHKEADKRGK 816

Query: 661 IYDRANSSFLFDLNDQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERI 720
           IYDR N SFLF+LNDQ+VLDAYRKGDKLKFANHS  PNCYAKV++VAGDHRVGIFAKERI
Sbjct: 817 IYDRENCSFLFNLNDQFVLDAYRKGDKLKFANHSPEPNCYAKVIMVAGDHRVGIFAKERI 876

Query: 721 EASEELFYDYRYGPDQAPAWARKPE--GSKRDD-ASISQGRAKK 744
            A EELFYDYRY PD+APAWA+KPE  GSK+D+  + S GR KK
Sbjct: 877 LAGEELFYDYRYEPDRAPAWAKKPEAPGSKKDENVTPSVGRPKK 900

BLAST of Tan0010400 vs. TAIR 10
Match: AT1G02580.1 (SET domain-containing protein )

HSP 1 Score: 383.6 bits (984), Expect = 3.5e-106
Identity = 263/738 (35.64%), Postives = 351/738 (47.56%), Query Frame = 0

Query: 1   MAEDQSVVGRRRIYYDQHGSEALICSDSEEEVAEPED------EKHEFSEGEDRVLWIII 60
           MAE  SV+G+R+IYY     EAL  S  E+E  E ED      EK EFSE  DR +W + 
Sbjct: 148 MAESDSVIGKRQIYY--LNGEALELSSEEDEEDEEEDEEEIKKEKCEFSEDVDRFIWTVG 207

Query: 61  QEHGLGEDVL-QILSHSIGCTTSEIQERYNVLKERNYRTDSSSKALEKFKSNKGISLYKS 120
           Q++GL + V+ + L+  +    S+I ERYN LK +N  T   +  L            K+
Sbjct: 208 QDYGLDDLVVRRALAKYLEVDVSDILERYNELKLKNDGTAGEASDLTS----------KT 267

Query: 121 LSSTLDSF-DNLFCRRCMLFDCRLHGCSQSLIYPSEKLLYWPEHEEERKPCSNQCFLQRT 180
           +++    F D   CRRCM+FDC +H   +     SE      E +E+R+PCS  C+L   
Sbjct: 268 ITTAFQDFADRRHCRRCMIFDCHMHEKYEPESRSSEDKSSLFE-DEDRQPCSEHCYL--- 327

Query: 181 QRKTPDQRNKRPRSSKHEESSAHVDAEIIQDERCLTGKLSSITSEGVSVSEMTVGLNLDA 240
                                                K+ S+T                 
Sbjct: 328 -------------------------------------KVRSVT----------------- 387

Query: 241 SVGPATNPRNGAKQRDVKHQNKVSVSNDPELISNNSPDCKKQKLLSATDVANAPTDTIPE 300
                        + D    N  S+SN                      V + P +T+  
Sbjct: 388 -------------EADHVMDNDNSISN--------------------KIVVSDPNNTM-- 447

Query: 301 LCSTSTKHSHEDIRGLQKIELQRNEISLSEAIGQAEEKIPPSDIASCDNLQDTSRCRTVE 360
                                                                       
Sbjct: 448 ------------------------------------------------------------ 507

Query: 361 VKELSTSKLLADSVSEQVEGTCGSSEWKLMEKELYMKGIEIFGRNSCLISRNLLSGLKTC 420
                                     W  +EK+LY+KGIEIFGRNSC ++ N+L GLKTC
Sbjct: 508 --------------------------WTPVEKDLYLKGIEIFGRNSCDVALNILRGLKTC 567

Query: 421 MEVFNYMHNGGASTSQSSNRMPSSNADDNGKTDIDYMEQDMRTRSRLLRKRGKARKLKYS 480
           +E++NYM      T        S + +   +      ++  R  SR +RK+ + RK    
Sbjct: 568 LEIYNYMREQDQCTM-------SLDLNKTTQRHNQVTKKVSRKSSRSVRKKSRLRKY--- 627

Query: 481 WKSAGHPSFWKRIADGKNQSCKQYTPCGCLSSCGKQCPCLHNGTCCEKYCGCSKSCKNRF 540
              A +P   K+   G+ +  K YTPC C S CG+QCPCL +  CCEKYCGCSK C NRF
Sbjct: 628 ---ARYPPALKKTTSGEAKFYKHYTPCTCKSKCGQQCPCLTHENCCEKYCGCSKDCNNRF 675

Query: 541 RGCHCAKSQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGPDGLLGEPPRQGDGQCGNMRL 600
            GC+CA  QC +RQCPCFAA RECDPD+CR+C +SCG   DG LGE P Q   QC NM+ 
Sbjct: 688 GGCNCAIGQCTNRQCPCFAANRECDPDLCRSCPLSCG---DGTLGETPVQ--IQCKNMQF 675

Query: 601 LLRQQQRILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRANSSFL 660
           LL+  ++IL+ KSDV GWGAF  +++ KN+YLGEYTGELI+H EA++RG+I DR  SS+L
Sbjct: 748 LLQTNKKILIGKSDVHGWGAFTWDSLKKNEYLGEYTGELITHDEANERGRIEDRIGSSYL 675

Query: 661 FDLNDQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEELFYDY 720
           F LNDQ  +DA RKG++ KF NHS+ PNCYAK+M+V GD R+G+FA+  IE  EELF+DY
Sbjct: 808 FTLNDQLEIDARRKGNEFKFLNHSARPNCYAKLMIVRGDQRIGLFAERAIEEGEELFFDY 675

Query: 721 RYGPDQAPAWARKPEGSK 731
            YGP+ A  W+R  E  K
Sbjct: 868 CYGPEHAD-WSRGREPRK 675

BLAST of Tan0010400 vs. TAIR 10
Match: AT4G30860.1 (SET domain group 4 )

HSP 1 Score: 103.2 bits (256), Expect = 9.2e-22
Identity = 61/180 (33.89%), Postives = 95/180 (52.78%), Query Frame = 0

Query: 549 CFAAGRECDPD-VCRNCWVSCGDG---PDGLLGEPPRQGDGQCGNMRLLLRQQQRILLAK 608
           C   G  CD   VCR   +SC  G   P+             CGN     R++++I + K
Sbjct: 287 CTNCGPNCDRSCVCRVQCISCSKGCSCPE------------SCGNRP--FRKEKKIKIVK 346

Query: 609 SDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDRANSS----FLFDLNDQYV 668
           ++  GWG     ++NK D++ EY GE+IS  + ++R  ++D  +      ++ ++   + 
Sbjct: 347 TEHCGWGVEAAESINKEDFIVEYIGEVISDAQCEQR--LWDMKHKGMKDFYMCEIQKDFT 406

Query: 669 LDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEELFYDYR---YGPD 718
           +DA  KG+  +F NHS NPNC  +   V G+ RVG+FA  +IEA E L YDYR   +GP+
Sbjct: 407 IDATFKGNASRFLNHSCNPNCVLEKWQVEGETRVGVFAARQIEAGEPLTYDYRFVQFGPE 450

BLAST of Tan0010400 vs. TAIR 10
Match: AT5G42400.1 (SET domain protein 25 )

HSP 1 Score: 97.1 bits (240), Expect = 6.6e-20
Identity = 46/127 (36.22%), Postives = 75/127 (59.06%), Query Frame = 0

Query: 590  MRLLLRQQQRILLAKSDVAGWGAFLKNAVNKNDYLGEYTGELISHREADKRGKIYDR--A 649
            M  L  +++ +   +S +  WG      +   D++ EY GELI    ++ R + Y++   
Sbjct: 1258 MSQLKARKKHLRFQQSKIHDWGLVALEPIEAEDFVIEYVGELIRSSISEIRERQYEKMGI 1317

Query: 650  NSSFLFDLNDQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKERIEASEE 709
             SS+LF L+D YVLDA ++G   +F NHS  PNCY K++ V G  ++ I+AK  I+A EE
Sbjct: 1318 GSSYLFRLDDGYVLDATKRGGIARFINHSCEPNCYTKIISVEGKKKIFIYAKRHIDAGEE 1377

Query: 710  LFYDYRY 715
            + Y+Y++
Sbjct: 1378 ISYNYKF 1384