Tan0010383 (gene) Snake gourd v1

Overview
NameTan0010383
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionWAT1-related protein
LocationLG08: 6872296 .. 6874517 (-)
RNA-Seq ExpressionTan0010383
SyntenyTan0010383
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGGAGATAATATTACAGCGATGTTGGGGATGATTCTTTTGCAAATTTGCTACGGTGGTTTAAACATTATCTCAAAGCTCGCCATGCAATCTGGAATGAACCCTTTTGTTCTTCTCACTTACAGACAGATTTTTGGCACTTTCGCCATTGCTCCTTTTGCCTTTTTCACTGAAAGGTGTAATTTCATTTTCATGCCTTTTTTTTCTTTTTTTTTGTTTCAAAAAGAAGAATATTAATCTTTTGTGGTTCTTGTTTTGTTTTTGTTTGTCATATTCTTGTAAGGATTCATGTAATCTCTCAAAGGAAATTAAACAAAGTTTGTTTCTTTTTTTCAGGAAAACAAGGCCAAAGATTACTTTTCCAATTTTGTTTCAAATTCTATTGTGTTCCTTATCAGGGTGAGTAAAATTAAAAATTAATTAAAGGGTCTTCTTCAAGTTTTTAAATTTTGCAACTTTTTTTTTTTTTTTTTTTGAGAATTAATGAAAATGAGAAATTAAGAACTGAAATAAATTTAATTTAATGTTTTTGGCAGAGCAACAGCAAACCAGATATTCTTCTTTGTTGGGTTGAAGTATACAAACCCAACAGTTTCAAGTGCTATGACCAATGTTCTTCCAGCTGCTACTTTTATTCTTGCTGTTCTTTTCAGGTAATTAAATTATTTTTTTTGCCCTTTTTTGTTTTTAACTTTGGAAATTCACTGCAAAAACGGTTTGTTAGAGTATATGGATAAAAAAAAATTAAATATATATACACAATAATTTATCGACTTAAACTTTTGAATTTAGTGGTAAGTCGCCCACTGGACCATATAATAATTAATTTATTAGCTTAGTTTAAGGGTAATATGGCAATTGTTGCTATAATCTGTTAGCTTAAATTTTTTGGTTTAGCTGTAGTTTAAGCGTAATACGGTAACTTTGGAAATTCACTGCAAAATGGATGTATTATAGTATATGAATAAAAAGATTAAATATATATACATGATAATTTATCGACTTAAACTTTTGAGTTTAGCGGTCTTAAGTCATTTGTTGGAATTTTCATTGACATGAGTGTTGTTGTAATCGGTTAGCTTAAGTTTTTTGGTTTAGCGGTAGTTTAAGGGTAATATGGTAATTTTGGCAGACAAGAAAGTGTAAGAATCAAGACCAAGCCTGGTTTTGCAAAGGTAGCAGGGACAATTGTGTGTGTATGTGGGGCAATGCTGCTGTCTTTTTACCATGGACACACCATTGGCTTAGGTGAATCCAAAATTCACTGGCCATATATTGAGAGAATGATGGAAAGAAACCCGAACCGTCGAGAAAACCGCATTCTCGGCTCGATTTTGTTACTTTGCAGCTCTTTCGCTTGGGCGTTATGGTTTGTGATTCAAGTAAGTTGCCGTGATCATCCTCGGTTAGCTTAGTTTGTGGCATTTTTCAATCTAAAGGGCATTTCGTTTCGAAGTTTTAAACTATACTTTGTATATATATATATACTTTGTTTGTTAGGCAAGATTGAGTGCCAAGTTTAAAGCTCCCTACACAAGTACTACATTGTTGTGTTTCATGGCCTTTTTCGAATGTGGATTCGTAGCTCTAATTTTAGAGCGCAACATTGCTGCTTGGTCTTTGAAAAGTACGATCCGACTCGTCGCAGTTCTTTACACGGTATGCCCCATACTTAAATTGCTCATTACACGGTGTTGTTTCGGAATGTAAACACTTATGACATGTTTTGTAGGGAGTTGTGTGTTCTGGATTGACATTTAGCATCACTTCATGGATTATACAAAGGAAAGGCCCTCTCTATGTCTCCATTTTCACCCCTTTATTGCTTATCATTGTGGCCATCCTTAGTTGGGCTCTCCTCCACGAACAACTCTACGTCGGAACGTACGTATATCCATGCATTTCTATTTCGATCCAAAACAAAATCATTATCAAACGGTCATTTCGCTTCATTACTATCTTCATTATCATAATCTAGTTGTGATTTAGCAATGGTATTACTGTTTTAAAACACATATTGATCTTTGAACAGAGTTATTGGCTCAGTTTTGATCATCATTGGGCTTTATGCTGTGCTATGGGGAAAGTCCAAAGAGATGAATAAGCTTCAAGATCACAACATGGAAAATCCAACAATGATAGAGAAGCCAAATGGGAATATCAGTCATAATATTGATGAGAAAAATGACATGGAATTGCAAATCACATACATCAAATGA

mRNA sequence

ATGGTGGGAGATAATATTACAGCGATGTTGGGGATGATTCTTTTGCAAATTTGCTACGGTGGTTTAAACATTATCTCAAAGCTCGCCATGCAATCTGGAATGAACCCTTTTGTTCTTCTCACTTACAGACAGATTTTTGGCACTTTCGCCATTGCTCCTTTTGCCTTTTTCACTGAAAGGAAAACAAGGCCAAAGATTACTTTTCCAATTTTGTTTCAAATTCTATTGTGTTCCTTATCAGGAGCAACAGCAAACCAGATATTCTTCTTTGTTGGGTTGAAGTATACAAACCCAACAGTTTCAAGTGCTATGACCAATGTTCTTCCAGCTGCTACTTTTATTCTTGCTGTTCTTTTCAGACAAGAAAGTGTAAGAATCAAGACCAAGCCTGGTTTTGCAAAGGTAGCAGGGACAATTGTGTGTGTATGTGGGGCAATGCTGCTGTCTTTTTACCATGGACACACCATTGGCTTAGGTGAATCCAAAATTCACTGGCCATATATTGAGAGAATGATGGAAAGAAACCCGAACCGTCGAGAAAACCGCATTCTCGGCTCGATTTTGTTACTTTGCAGCTCTTTCGCTTGGGCGTTATGGTTTGTGATTCAAGCAAGATTGAGTGCCAAGTTTAAAGCTCCCTACACAAGTACTACATTGTTGTGTTTCATGGCCTTTTTCGAATGTGGATTCGTAGCTCTAATTTTAGAGCGCAACATTGCTGCTTGGTCTTTGAAAAGTACGATCCGACTCGTCGCAGTTCTTTACACGGGAGTTGTGTGTTCTGGATTGACATTTAGCATCACTTCATGGATTATACAAAGGAAAGGCCCTCTCTATGTCTCCATTTTCACCCCTTTATTGCTTATCATTGTGGCCATCCTTAGTTGGGCTCTCCTCCACGAACAACTCTACGTCGGAACAGTTATTGGCTCAGTTTTGATCATCATTGGGCTTTATGCTGTGCTATGGGGAAAGTCCAAAGAGATGAATAAGCTTCAAGATCACAACATGGAAAATCCAACAATGATAGAGAAGCCAAATGGGAATATCAGTCATAATATTGATGAGAAAAATGACATGGAATTGCAAATCACATACATCAAATGA

Coding sequence (CDS)

ATGGTGGGAGATAATATTACAGCGATGTTGGGGATGATTCTTTTGCAAATTTGCTACGGTGGTTTAAACATTATCTCAAAGCTCGCCATGCAATCTGGAATGAACCCTTTTGTTCTTCTCACTTACAGACAGATTTTTGGCACTTTCGCCATTGCTCCTTTTGCCTTTTTCACTGAAAGGAAAACAAGGCCAAAGATTACTTTTCCAATTTTGTTTCAAATTCTATTGTGTTCCTTATCAGGAGCAACAGCAAACCAGATATTCTTCTTTGTTGGGTTGAAGTATACAAACCCAACAGTTTCAAGTGCTATGACCAATGTTCTTCCAGCTGCTACTTTTATTCTTGCTGTTCTTTTCAGACAAGAAAGTGTAAGAATCAAGACCAAGCCTGGTTTTGCAAAGGTAGCAGGGACAATTGTGTGTGTATGTGGGGCAATGCTGCTGTCTTTTTACCATGGACACACCATTGGCTTAGGTGAATCCAAAATTCACTGGCCATATATTGAGAGAATGATGGAAAGAAACCCGAACCGTCGAGAAAACCGCATTCTCGGCTCGATTTTGTTACTTTGCAGCTCTTTCGCTTGGGCGTTATGGTTTGTGATTCAAGCAAGATTGAGTGCCAAGTTTAAAGCTCCCTACACAAGTACTACATTGTTGTGTTTCATGGCCTTTTTCGAATGTGGATTCGTAGCTCTAATTTTAGAGCGCAACATTGCTGCTTGGTCTTTGAAAAGTACGATCCGACTCGTCGCAGTTCTTTACACGGGAGTTGTGTGTTCTGGATTGACATTTAGCATCACTTCATGGATTATACAAAGGAAAGGCCCTCTCTATGTCTCCATTTTCACCCCTTTATTGCTTATCATTGTGGCCATCCTTAGTTGGGCTCTCCTCCACGAACAACTCTACGTCGGAACAGTTATTGGCTCAGTTTTGATCATCATTGGGCTTTATGCTGTGCTATGGGGAAAGTCCAAAGAGATGAATAAGCTTCAAGATCACAACATGGAAAATCCAACAATGATAGAGAAGCCAAATGGGAATATCAGTCATAATATTGATGAGAAAAATGACATGGAATTGCAAATCACATACATCAAATGA

Protein sequence

MVGDNITAMLGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTERKTRPKITFPILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFRQESVRIKTKPGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMERNPNRRENRILGSILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERNIAAWSLKSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWALLHEQLYVGTVIGSVLIIIGLYAVLWGKSKEMNKLQDHNMENPTMIEKPNGNISHNIDEKNDMELQITYIK
Homology
BLAST of Tan0010383 vs. ExPASy Swiss-Prot
Match: Q8GXB4 (WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.4e-105
Identity = 186/358 (51.96%), Postives = 255/358 (71.23%), Query Frame = 0

Query: 1   MVGDNITAMLGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTER 60
           M   ++   L M+L+QI Y G+NI SK+AM++GM P +L+ YRQIF T A  P AFF ER
Sbjct: 1   MAKSDMLPFLAMVLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATFPVAFFLER 60

Query: 61  KTRPKITFPILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFR 120
           KTRPKIT  IL Q+  CS++GAT NQ+ +FVGL+ ++PT++ A+TN+LPA TF+LA +FR
Sbjct: 61  KTRPKITLRILVQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVTFLLAAIFR 120

Query: 121 QESVRIKTKPGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMER-NPNRR 180
           QE+V IK   G AKV GT+VCV GAM+LSFYHGHTIG+GESKIHW Y E + +  + +  
Sbjct: 121 QETVGIKKASGQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENITKHGSSSGH 180

Query: 181 ENRILGSILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERNI 240
            N  LG  L++ ++ +WA WF+IQ ++S  F APYTST L+C M   +CG +ALI +  I
Sbjct: 181 SNFFLGPFLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQCGAIALISDHTI 240

Query: 241 AAWSLKSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWALL 300
           + WSL S +R ++ LY GVV S L F + SW +QRKGPLYVS+F+PLLL++VAI SWALL
Sbjct: 241 SDWSLSSPLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWALL 300

Query: 301 HEQLYVGTVIGSVLIIIGLYAVLWGKSKEMNKLQDHNMENPTMIEKPNGNISHNIDEK 358
            E+LY GT +GS L++IGLY VLWGK +E+++ ++   +      K     + +I+ +
Sbjct: 301 EEKLYTGTFMGSALVVIGLYGVLWGKDREVSEKEEEREKVKQQNHKVKSESNEDIESR 358

BLAST of Tan0010383 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.0e-67
Identity = 139/324 (42.90%), Postives = 215/324 (66.36%), Query Frame = 0

Query: 12  MILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTERKTRPKITFPIL 71
           MI LQ  Y G+NII+K+++ +GM+ +VL+ YR    T  IAPFAFF ERK +PKITF I 
Sbjct: 22  MISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKITFSIF 81

Query: 72  FQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFRQESVRIKTKPG 131
            Q+ +  L G   +Q F+++GLKYT+PT S AM+N+LPA TFILAVLFR E + +K    
Sbjct: 82  MQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLWC 141

Query: 132 FAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMERNPNRRENR------ILG 191
            AK+AGT+V V GAML++ Y G  + L  +K  + +I+     N    +N       + G
Sbjct: 142 QAKIAGTVVTVAGAMLMTIYKGPIVELFWTK--YMHIQDSSHANTTSSKNSSSDKEFLKG 201

Query: 192 SILLLCSSFAWALWFVIQARLSAKF-KAPYTSTTLLCFMAFFECGFVALILERNIAAWSL 251
           SILL+ ++ AWA  FV+QA++   + K   + TTL+CF+   +   V  ++E N +AW +
Sbjct: 202 SILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAWRI 261

Query: 252 KSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWALLHEQLY 311
              + L+A  Y+G+V S +++ +   +++++GP++ + F+PL+++IVA++   +L E+++
Sbjct: 262 GWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIF 321

Query: 312 VGTVIGSVLIIIGLYAVLWGKSKE 329
           +G VIG+VLI+IGLYAVLWGK KE
Sbjct: 322 LGGVIGAVLIVIGLYAVLWGKQKE 343

BLAST of Tan0010383 vs. ExPASy Swiss-Prot
Match: Q9LI65 (WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.7e-64
Identity = 128/323 (39.63%), Postives = 206/323 (63.78%), Query Frame = 0

Query: 8   AMLGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTERKTRPKIT 67
           A+L M ++ I    +N++ K  +  G+N  V  TYR   GT  + PFA F ER  RPK+T
Sbjct: 11  AVLMMSMINIGLSVVNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLERHNRPKLT 70

Query: 68  FPILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFRQESVRIK 127
             IL  +   +L G +  Q FF +GL+YT+ T S A +N++P+ TF LA++FRQE++ IK
Sbjct: 71  GRILCSLFFSALLGTSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFRQETLNIK 130

Query: 128 TKPGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMERNPN--RRENRILG 187
           +  G AK+ GT++C+CGA++L+ Y G  +    S+ H  ++E     +      +   +G
Sbjct: 131 SNVGRAKLLGTMICICGALVLTLYKGTAL----SREHSTHMETHTRTDSTGAMTQKWAMG 190

Query: 188 SILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERNIAAWSLK 247
           SI+L+ S   W+ WF++QA++S  +   YTSTT+L F    +   ++LI ER+ + W +K
Sbjct: 191 SIMLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMWVVK 250

Query: 248 STIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWALLHEQLYV 307
              +++A+LY+G+V SGL +   SW ++++G ++ S F PL+ +  AI S++ LHEQ+Y 
Sbjct: 251 DKFQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQIYC 310

Query: 308 GTVIGSVLIIIGLYAVLWGKSKE 329
           G+VIGS++II+GLY +LWGKSK+
Sbjct: 311 GSVIGSMVIIVGLYILLWGKSKD 329

BLAST of Tan0010383 vs. ExPASy Swiss-Prot
Match: Q9FNA5 (WAT1-related protein At5g13670 OS=Arabidopsis thaliana OX=3702 GN=At5g13670 PE=2 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.8e-60
Identity = 132/335 (39.40%), Postives = 202/335 (60.30%), Query Frame = 0

Query: 10  LGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTERKTRPKITFP 69
           + ++ +Q  Y  ++I++KLA+  GM+P VL+ YR    +  I PFA   ER TRPK+TF 
Sbjct: 10  IAIVFIQCLYALMSIVAKLALNKGMSPHVLVAYRMAVASALITPFALILERNTRPKLTFK 69

Query: 70  ILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFRQESVRIKTK 129
           IL QI + SL      Q  ++ G+K T  T +SA+ N LPA TFI+A +F+ E V I+ +
Sbjct: 70  ILLQIAILSLFEPVVEQNLYYSGMKLTTATFTSALCNALPAMTFIMACVFKLEKVTIERR 129

Query: 130 PGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMERNPNRRENRI------ 189
              AK+ GT+V + GAML++F  G+ I L       P+       N +    RI      
Sbjct: 130 HSQAKLVGTMVAIGGAMLMTFVKGNVIEL-------PWTSNSRGLNGHTHAMRIPKQADI 189

Query: 190 -LGSILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILER-NIAA 249
             GSI+L+ S F+W+ + ++QA++ A++KA  + T L+C M   E   + LI ER N++ 
Sbjct: 190 ARGSIMLVASCFSWSCYIILQAKILAQYKAELSLTALMCIMGMLEATVMGLIWERKNMSV 249

Query: 250 WSLKSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWALLHE 309
           W +   + L+A +Y G+V SGL + +  W  + +GP++VS F PL +++VAILS  +  E
Sbjct: 250 WKINPDVTLLASIYGGLV-SGLAYYVIGWASKERGPVFVSAFNPLSMVLVAILSTFVFLE 309

Query: 310 QLYVGTVIGSVLIIIGLYAVLWGKSKEMNKLQDHN 337
           ++YVG VIGSV+I+IG+Y VLWGKSK+   +   N
Sbjct: 310 KVYVGRVIGSVVIVIGIYLVLWGKSKDKGGMLQPN 336

BLAST of Tan0010383 vs. ExPASy Swiss-Prot
Match: F4IJ08 (WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 6.9e-60
Identity = 128/356 (35.96%), Postives = 218/356 (61.24%), Query Frame = 0

Query: 12  MILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTERKTRPKITFPIL 71
           M+ LQ  Y G+N+++K  +  GM+ +VL+ YR  F T AIAPFA  +ERK R K+TFPI 
Sbjct: 15  MVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVRSKMTFPIF 74

Query: 72  FQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFRQESVRIKTKPG 131
            +I L +L G   +Q  +++GLK T+PT SSA++N++PA T ILA LFR E V ++    
Sbjct: 75  MRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEKVEMRKVRC 134

Query: 132 FAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMERNPNRRENRILGSILLLC 191
             KV GT+V V G++L+ FY G  I    S         +   +     + +  ++ LL 
Sbjct: 135 LVKVMGTLVTVVGSILMIFYKGPFINFFRS--------HLTAASSPPTADYLKAAVFLLL 194

Query: 192 SSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERNIAAWSLKSTIRLV 251
           +S +WA +FV+QA    K+ A  + +T++CFM   +   +A ++E N +A ++   + L+
Sbjct: 195 ASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNPSALNIGFDMNLL 254

Query: 252 AVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWALLHEQLYVGTVIGS 311
           A  Y G++ S + + +   ++QRKGP++V+ F PL+++IV+I+S+ +L + +Y+G VIG 
Sbjct: 255 ASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGIYLGGVIGV 314

Query: 312 VLIIIGLYAVLWGKSKEMNKLQDHNMENPTMIEKPNGN----ISHNIDEKNDMELQ 364
           V++++G+YAVLWGK  + +  +  + +N   ++  +GN    I   IDE ++ +++
Sbjct: 315 VVLMVGVYAVLWGKHVDDDGEETRHEDNVVAVKCCSGNNGLTIMPKIDEADEEDVE 362

BLAST of Tan0010383 vs. NCBI nr
Match: XP_022156429.1 (WAT1-related protein At1g09380-like [Momordica charantia])

HSP 1 Score: 599.7 bits (1545), Expect = 1.6e-167
Identity = 306/364 (84.07%), Postives = 334/364 (91.76%), Query Frame = 0

Query: 2   VGDNITAMLGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTERK 61
           +G++ITA+LGM++LQICY GLNIISKLAMQSGMNP VLLTYRQIFGT AIAPFAFFTERK
Sbjct: 1   MGEDITAILGMVVLQICYAGLNIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFFTERK 60

Query: 62  TRPKITFPILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFRQ 121
           TRPKI+  +L QILLCSLSGATANQI FF+GLKYTNPTVSSAM N+LPAATFILAVLFRQ
Sbjct: 61  TRPKISVAVLIQILLCSLSGATANQILFFIGLKYTNPTVSSAMANILPAATFILAVLFRQ 120

Query: 122 ESVRIKTKPGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMERNPNRREN 181
           ESVRIKTKPG AKV GTI+CVCGAMLLSFYHGHTIGLGESKIHW YIERM +RNPNR+ N
Sbjct: 121 ESVRIKTKPGLAKVTGTIICVCGAMLLSFYHGHTIGLGESKIHWAYIERMRDRNPNRQGN 180

Query: 182 RILGSILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERNIAA 241
            ++GSILLLCSSFAWALWFVIQARLS KFKAPYTSTTLLCFMA F+CG +A+I E NIAA
Sbjct: 181 HVVGSILLLCSSFAWALWFVIQARLSVKFKAPYTSTTLLCFMASFQCGIIAVISEHNIAA 240

Query: 242 WSLKSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWALLHE 301
           WSLKST RLVA LYTGVVCS LTFSITSWIIQRKGPLYV+IFTPLLLIIVAILSWALL +
Sbjct: 241 WSLKSTSRLVAALYTGVVCSALTFSITSWIIQRKGPLYVAIFTPLLLIIVAILSWALLGD 300

Query: 302 QLYVGTVIGSVLIIIGLYAVLWGKSKEMNKLQDHNMENPTMIEKPNGNISHNIDEKNDME 361
           +LYVGTVIGSVLIIIGLYAVLWGKSKEM KLQDHNME PT+ + PNG   +NIDEK+DME
Sbjct: 301 KLYVGTVIGSVLIIIGLYAVLWGKSKEM-KLQDHNMEKPTIEKLPNGT-HNNIDEKDDME 360

Query: 362 LQIT 366
           +Q++
Sbjct: 361 MQLS 362

BLAST of Tan0010383 vs. NCBI nr
Match: XP_038891245.1 (WAT1-related protein At1g09380 [Benincasa hispida])

HSP 1 Score: 579.7 bits (1493), Expect = 1.8e-161
Identity = 300/359 (83.57%), Postives = 323/359 (89.97%), Query Frame = 0

Query: 12  MILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTERKTRPKITFPIL 71
           MI+LQICY G+NIISKLAMQSGMNP VLLTYRQIFGT AIAPFAFFTERKTRPKITF +L
Sbjct: 1   MIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFFTERKTRPKITFTVL 60

Query: 72  FQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFRQESVRIKTKPG 131
            QI +CSLSGAT NQIFFFVGLKYTNPT+SSAM NVLPAATFILAV+FRQESVRIKTK G
Sbjct: 61  IQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVIFRQESVRIKTKSG 120

Query: 132 FAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMM-ERNPNRRENR-ILGSILL 191
           FAKV GTI+CVCGAMLLSFYHGHTI LGESKIHWPY+ERM+ E NP  R+ + +LGS+LL
Sbjct: 121 FAKVTGTILCVCGAMLLSFYHGHTIDLGESKIHWPYVERMIKETNPTNRQGKCVLGSVLL 180

Query: 192 LCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERNIAAWSLKSTIR 251
           L SSFAWALWFVIQARLS KFKAPYTSTTLLCFMAFF+CG +A+I E N+AAWSLKSTIR
Sbjct: 181 LLSSFAWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHNVAAWSLKSTIR 240

Query: 252 LVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWALLHEQLYVGTVI 311
           LVA LY GVVCS LTFSITSWIIQRKGPLYVSIF+PLLLIIVAILSWALLH+QLY+GTVI
Sbjct: 241 LVAALYAGVVCSALTFSITSWIIQRKGPLYVSIFSPLLLIIVAILSWALLHQQLYIGTVI 300

Query: 312 GSVLIIIGLYAVLWGKSKEMNKLQDHNMENPTMIEKPNGNISHNIDEKNDMELQITYIK 369
           GSVLIIIGLYAVLWGKSKEM     HNME PT IEKP+G  SH I+EK+DMELQIT IK
Sbjct: 301 GSVLIIIGLYAVLWGKSKEMKLEDHHNMEKPT-IEKPDG--SHIIEEKDDMELQITNIK 356

BLAST of Tan0010383 vs. NCBI nr
Match: XP_008453225.2 (PREDICTED: WAT1-related protein At1g09380 [Cucumis melo] >KAA0048114.1 WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 570.5 bits (1469), Expect = 1.1e-158
Identity = 301/372 (80.91%), Postives = 328/372 (88.17%), Query Frame = 0

Query: 1   MVGDNITAMLGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTER 60
           M GD+ITA++GMI+LQICY G+NIISKLAMQSGMNP VLLTYRQIFGT AIAPFAFFTER
Sbjct: 1   MEGDDITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFFTER 60

Query: 61  KTRPKITFPILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFR 120
           KTRPKITF +LFQI +CSLSGAT NQIFFFVGLKYTNPT+SSAM NVLPAATFILAVLFR
Sbjct: 61  KTRPKITFTVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFR 120

Query: 121 QESVRIKTKPGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMERN--PNR 180
           QESVRIKTK GFAKV GTIVCV GAMLLSFYHGH I LGESKIHW Y+ER+++     N 
Sbjct: 121 QESVRIKTKSGFAKVIGTIVCVSGAMLLSFYHGHIINLGESKIHWSYVERLIKETTPTNG 180

Query: 181 RENRILGSILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERN 240
           +   +LGSILLL SSFAWALWFVIQARLS KFKAPYTST LLCFMAFF+CG +A+I E N
Sbjct: 181 QGKHVLGSILLLLSSFAWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHN 240

Query: 241 IAAWSLKSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWAL 300
           IAAWSLKSTIRL+A LY GVVCS LTFSITSW IQRKGPLYVSIF+PLLLIIVAILSWAL
Sbjct: 241 IAAWSLKSTIRLIAALYAGVVCSALTFSITSWAIQRKGPLYVSIFSPLLLIIVAILSWAL 300

Query: 301 LHEQLYVGTVIGSVLIIIGLYAVLWGKSKEMNKLQD--HNMENPTMIEKPNGNISHNIDE 360
           LH+QL+VGTVIGSVLIIIGLYAVLWGKSKEM K++D  HNME  T IEK NG  SH ++E
Sbjct: 301 LHQQLHVGTVIGSVLIIIGLYAVLWGKSKEM-KVEDHQHNMEKAT-IEKHNG--SHIVEE 360

Query: 361 KNDMELQITYIK 369
           K+D+ELQIT IK
Sbjct: 361 KDDLELQITNIK 368

BLAST of Tan0010383 vs. NCBI nr
Match: XP_022946709.1 (WAT1-related protein At1g09380 [Cucurbita moschata])

HSP 1 Score: 567.8 bits (1462), Expect = 6.9e-158
Identity = 293/370 (79.19%), Postives = 323/370 (87.30%), Query Frame = 0

Query: 1   MVGDNITAMLGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTER 60
           M GD IT ++GM++LQ+CY GL+I SKLAMQSGMNP VLLTYRQ FGT AIAPFAFFTER
Sbjct: 1   MEGD-ITTIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTER 60

Query: 61  KTRPKITFPILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFR 120
           KTRPKITFP+L QI +CSLSGATANQIFFFVGLK TNPTVSSAM NVLPAATFILAV+FR
Sbjct: 61  KTRPKITFPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFR 120

Query: 121 QESVRIKTKPGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMER-NP-NR 180
           QESVRIKTKPG AKV GTIVCVCGAMLLSFYHGHTIGLGESKIHWPY+ER+M + NP NR
Sbjct: 121 QESVRIKTKPGLAKVMGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNR 180

Query: 181 RENRILGSILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERN 240
           +ENR LGS+LLLCSSF+WALWFVIQARLS KFKAPYTSTTLLCFMAFF+CG +A+I E N
Sbjct: 181 QENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHN 240

Query: 241 IAAWSLKSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWAL 300
           +AAWSLKS+IRL++ LYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAI SWAL
Sbjct: 241 VAAWSLKSSIRLISALYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWAL 300

Query: 301 LHEQLYVGTVIGSVLIIIGLYAVLWGKSKEMNKLQDHNMENPTMIEKPNGNISHNIDEKN 360
           LHEQLYVGTV+GS+LIIIGLY VLWGKSKEM   ++  +E  T           N  +K+
Sbjct: 301 LHEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEEKVEKAT-----------NGSQKD 358

Query: 361 DMELQITYIK 369
           D+ELQI  IK
Sbjct: 361 DVELQIINIK 358

BLAST of Tan0010383 vs. NCBI nr
Match: KAG7030108.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 566.2 bits (1458), Expect = 2.0e-157
Identity = 292/370 (78.92%), Postives = 322/370 (87.03%), Query Frame = 0

Query: 1   MVGDNITAMLGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTER 60
           M GD IT ++GM++LQ+CY GL+I SKLAMQSGMNP VLLTYRQ FGT AIAPFAFFTER
Sbjct: 1   MEGD-ITTIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTER 60

Query: 61  KTRPKITFPILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFR 120
           KTRPKITFP+L QI +CSLSGATANQIFFFVGLK TNPTVSSAM NVLPAATFILAV+FR
Sbjct: 61  KTRPKITFPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFR 120

Query: 121 QESVRIKTKPGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMER-NP-NR 180
           QESVRIKTKPG AKV GTIVC+CGAMLLSFYHGHTIGLGESK+HWPY+ER+M + NP NR
Sbjct: 121 QESVRIKTKPGLAKVMGTIVCICGAMLLSFYHGHTIGLGESKLHWPYVERIMSKTNPTNR 180

Query: 181 RENRILGSILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERN 240
           +ENR LGS+LLLCSSF+WALWFVIQARLS KFKAPYTSTTLLCFMAFF+CG +A+I E N
Sbjct: 181 QENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHN 240

Query: 241 IAAWSLKSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWAL 300
           +AAWSLKS IRLV+ LYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAI SWAL
Sbjct: 241 VAAWSLKSPIRLVSALYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWAL 300

Query: 301 LHEQLYVGTVIGSVLIIIGLYAVLWGKSKEMNKLQDHNMENPTMIEKPNGNISHNIDEKN 360
           LHEQLYVGTV+GS+LIIIGLY VLWGKSKEM   ++  +E  T           N  +K+
Sbjct: 301 LHEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEEKVEKAT-----------NGSQKD 358

Query: 361 DMELQITYIK 369
           D+ELQI  IK
Sbjct: 361 DVELQIINIK 358

BLAST of Tan0010383 vs. ExPASy TrEMBL
Match: A0A6J1DTF9 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111023321 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 8.0e-168
Identity = 306/364 (84.07%), Postives = 334/364 (91.76%), Query Frame = 0

Query: 2   VGDNITAMLGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTERK 61
           +G++ITA+LGM++LQICY GLNIISKLAMQSGMNP VLLTYRQIFGT AIAPFAFFTERK
Sbjct: 1   MGEDITAILGMVVLQICYAGLNIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFFTERK 60

Query: 62  TRPKITFPILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFRQ 121
           TRPKI+  +L QILLCSLSGATANQI FF+GLKYTNPTVSSAM N+LPAATFILAVLFRQ
Sbjct: 61  TRPKISVAVLIQILLCSLSGATANQILFFIGLKYTNPTVSSAMANILPAATFILAVLFRQ 120

Query: 122 ESVRIKTKPGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMERNPNRREN 181
           ESVRIKTKPG AKV GTI+CVCGAMLLSFYHGHTIGLGESKIHW YIERM +RNPNR+ N
Sbjct: 121 ESVRIKTKPGLAKVTGTIICVCGAMLLSFYHGHTIGLGESKIHWAYIERMRDRNPNRQGN 180

Query: 182 RILGSILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERNIAA 241
            ++GSILLLCSSFAWALWFVIQARLS KFKAPYTSTTLLCFMA F+CG +A+I E NIAA
Sbjct: 181 HVVGSILLLCSSFAWALWFVIQARLSVKFKAPYTSTTLLCFMASFQCGIIAVISEHNIAA 240

Query: 242 WSLKSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWALLHE 301
           WSLKST RLVA LYTGVVCS LTFSITSWIIQRKGPLYV+IFTPLLLIIVAILSWALL +
Sbjct: 241 WSLKSTSRLVAALYTGVVCSALTFSITSWIIQRKGPLYVAIFTPLLLIIVAILSWALLGD 300

Query: 302 QLYVGTVIGSVLIIIGLYAVLWGKSKEMNKLQDHNMENPTMIEKPNGNISHNIDEKNDME 361
           +LYVGTVIGSVLIIIGLYAVLWGKSKEM KLQDHNME PT+ + PNG   +NIDEK+DME
Sbjct: 301 KLYVGTVIGSVLIIIGLYAVLWGKSKEM-KLQDHNMEKPTIEKLPNGT-HNNIDEKDDME 360

Query: 362 LQIT 366
           +Q++
Sbjct: 361 MQLS 362

BLAST of Tan0010383 vs. ExPASy TrEMBL
Match: A0A5A7TYK5 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001930 PE=3 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 5.2e-159
Identity = 301/372 (80.91%), Postives = 328/372 (88.17%), Query Frame = 0

Query: 1   MVGDNITAMLGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTER 60
           M GD+ITA++GMI+LQICY G+NIISKLAMQSGMNP VLLTYRQIFGT AIAPFAFFTER
Sbjct: 1   MEGDDITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFFTER 60

Query: 61  KTRPKITFPILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFR 120
           KTRPKITF +LFQI +CSLSGAT NQIFFFVGLKYTNPT+SSAM NVLPAATFILAVLFR
Sbjct: 61  KTRPKITFTVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFR 120

Query: 121 QESVRIKTKPGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMERN--PNR 180
           QESVRIKTK GFAKV GTIVCV GAMLLSFYHGH I LGESKIHW Y+ER+++     N 
Sbjct: 121 QESVRIKTKSGFAKVIGTIVCVSGAMLLSFYHGHIINLGESKIHWSYVERLIKETTPTNG 180

Query: 181 RENRILGSILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERN 240
           +   +LGSILLL SSFAWALWFVIQARLS KFKAPYTST LLCFMAFF+CG +A+I E N
Sbjct: 181 QGKHVLGSILLLLSSFAWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHN 240

Query: 241 IAAWSLKSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWAL 300
           IAAWSLKSTIRL+A LY GVVCS LTFSITSW IQRKGPLYVSIF+PLLLIIVAILSWAL
Sbjct: 241 IAAWSLKSTIRLIAALYAGVVCSALTFSITSWAIQRKGPLYVSIFSPLLLIIVAILSWAL 300

Query: 301 LHEQLYVGTVIGSVLIIIGLYAVLWGKSKEMNKLQD--HNMENPTMIEKPNGNISHNIDE 360
           LH+QL+VGTVIGSVLIIIGLYAVLWGKSKEM K++D  HNME  T IEK NG  SH ++E
Sbjct: 301 LHQQLHVGTVIGSVLIIIGLYAVLWGKSKEM-KVEDHQHNMEKAT-IEKHNG--SHIVEE 360

Query: 361 KNDMELQITYIK 369
           K+D+ELQIT IK
Sbjct: 361 KDDLELQITNIK 368

BLAST of Tan0010383 vs. ExPASy TrEMBL
Match: A0A1S3BWI1 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103494009 PE=3 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 5.2e-159
Identity = 301/372 (80.91%), Postives = 328/372 (88.17%), Query Frame = 0

Query: 1   MVGDNITAMLGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTER 60
           M GD+ITA++GMI+LQICY G+NIISKLAMQSGMNP VLLTYRQIFGT AIAPFAFFTER
Sbjct: 1   MEGDDITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFFTER 60

Query: 61  KTRPKITFPILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFR 120
           KTRPKITF +LFQI +CSLSGAT NQIFFFVGLKYTNPT+SSAM NVLPAATFILAVLFR
Sbjct: 61  KTRPKITFTVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFR 120

Query: 121 QESVRIKTKPGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMERN--PNR 180
           QESVRIKTK GFAKV GTIVCV GAMLLSFYHGH I LGESKIHW Y+ER+++     N 
Sbjct: 121 QESVRIKTKSGFAKVIGTIVCVSGAMLLSFYHGHIINLGESKIHWSYVERLIKETTPTNG 180

Query: 181 RENRILGSILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERN 240
           +   +LGSILLL SSFAWALWFVIQARLS KFKAPYTST LLCFMAFF+CG +A+I E N
Sbjct: 181 QGKHVLGSILLLLSSFAWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHN 240

Query: 241 IAAWSLKSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWAL 300
           IAAWSLKSTIRL+A LY GVVCS LTFSITSW IQRKGPLYVSIF+PLLLIIVAILSWAL
Sbjct: 241 IAAWSLKSTIRLIAALYAGVVCSALTFSITSWAIQRKGPLYVSIFSPLLLIIVAILSWAL 300

Query: 301 LHEQLYVGTVIGSVLIIIGLYAVLWGKSKEMNKLQD--HNMENPTMIEKPNGNISHNIDE 360
           LH+QL+VGTVIGSVLIIIGLYAVLWGKSKEM K++D  HNME  T IEK NG  SH ++E
Sbjct: 301 LHQQLHVGTVIGSVLIIIGLYAVLWGKSKEM-KVEDHQHNMEKAT-IEKHNG--SHIVEE 360

Query: 361 KNDMELQITYIK 369
           K+D+ELQIT IK
Sbjct: 361 KDDLELQITNIK 368

BLAST of Tan0010383 vs. ExPASy TrEMBL
Match: A0A6J1G4M7 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111450701 PE=3 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 3.4e-158
Identity = 293/370 (79.19%), Postives = 323/370 (87.30%), Query Frame = 0

Query: 1   MVGDNITAMLGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTER 60
           M GD IT ++GM++LQ+CY GL+I SKLAMQSGMNP VLLTYRQ FGT AIAPFAFFTER
Sbjct: 1   MEGD-ITTIMGMVILQVCYAGLSITSKLAMQSGMNPLVLLTYRQFFGTLAIAPFAFFTER 60

Query: 61  KTRPKITFPILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFR 120
           KTRPKITFP+L QI +CSLSGATANQIFFFVGLK TNPTVSSAM NVLPAATFILAV+FR
Sbjct: 61  KTRPKITFPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFR 120

Query: 121 QESVRIKTKPGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMER-NP-NR 180
           QESVRIKTKPG AKV GTIVCVCGAMLLSFYHGHTIGLGESKIHWPY+ER+M + NP NR
Sbjct: 121 QESVRIKTKPGLAKVMGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNR 180

Query: 181 RENRILGSILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERN 240
           +ENR LGS+LLLCSSF+WALWFVIQARLS KFKAPYTSTTLLCFMAFF+CG +A+I E N
Sbjct: 181 QENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHN 240

Query: 241 IAAWSLKSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWAL 300
           +AAWSLKS+IRL++ LYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAI SWAL
Sbjct: 241 VAAWSLKSSIRLISALYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWAL 300

Query: 301 LHEQLYVGTVIGSVLIIIGLYAVLWGKSKEMNKLQDHNMENPTMIEKPNGNISHNIDEKN 360
           LHEQLYVGTV+GS+LIIIGLY VLWGKSKEM   ++  +E  T           N  +K+
Sbjct: 301 LHEQLYVGTVLGSILIIIGLYGVLWGKSKEMKVEEEEKVEKAT-----------NGSQKD 358

Query: 361 DMELQITYIK 369
           D+ELQI  IK
Sbjct: 361 DVELQIINIK 358

BLAST of Tan0010383 vs. ExPASy TrEMBL
Match: A0A6J1KB70 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111493889 PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.1e-156
Identity = 289/370 (78.11%), Postives = 321/370 (86.76%), Query Frame = 0

Query: 1   MVGDNITAMLGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTER 60
           M GD ITA++GM++LQ CY GL+I SKLAMQSGM+P VLLTYRQ FGT AIAPFAFFTER
Sbjct: 16  MEGD-ITAIMGMVILQFCYAGLSITSKLAMQSGMSPLVLLTYRQFFGTLAIAPFAFFTER 75

Query: 61  KTRPKITFPILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFR 120
           KTRPKITFP+L QI +CSLSGATANQIFFFVGLK TNPTVSSAM NVLPAATFILAV+FR
Sbjct: 76  KTRPKITFPVLVQIFICSLSGATANQIFFFVGLKCTNPTVSSAMANVLPAATFILAVVFR 135

Query: 121 QESVRIKTKPGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMER-NP-NR 180
           QESVRIKTKPGFAKV GTIVC+CGAMLLSFYHGHTIGLGESKIHWPY+ER+M + NP NR
Sbjct: 136 QESVRIKTKPGFAKVMGTIVCICGAMLLSFYHGHTIGLGESKIHWPYVERIMSKTNPTNR 195

Query: 181 RENRILGSILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERN 240
           +ENR LGS+LLLCSSF+WALWFVIQARLS KFKAPYTSTTLLCFMAFF+CG +A+I E N
Sbjct: 196 QENRTLGSVLLLCSSFSWALWFVIQARLSVKFKAPYTSTTLLCFMAFFQCGLIAVISEHN 255

Query: 241 IAAWSLKSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWAL 300
           +AAWSL S IRL++ LYTG+VCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAI SW L
Sbjct: 256 VAAWSLNSPIRLISALYTGIVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAIFSWTL 315

Query: 301 LHEQLYVGTVIGSVLIIIGLYAVLWGKSKEMNKLQDHNMENPTMIEKPNGNISHNIDEKN 360
           LHEQLY+GTV+GS+LIIIGLY VLWGKSKEM   ++  +E  T           N  +K+
Sbjct: 316 LHEQLYIGTVLGSILIIIGLYGVLWGKSKEMKVEEEDKVEKAT-----------NGSQKD 373

Query: 361 DMELQITYIK 369
           D+ELQI  IK
Sbjct: 376 DVELQIINIK 373

BLAST of Tan0010383 vs. TAIR 10
Match: AT1G09380.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 383.6 bits (984), Expect = 1.7e-106
Identity = 186/358 (51.96%), Postives = 255/358 (71.23%), Query Frame = 0

Query: 1   MVGDNITAMLGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTER 60
           M   ++   L M+L+QI Y G+NI SK+AM++GM P +L+ YRQIF T A  P AFF ER
Sbjct: 1   MAKSDMLPFLAMVLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATFPVAFFLER 60

Query: 61  KTRPKITFPILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFR 120
           KTRPKIT  IL Q+  CS++GAT NQ+ +FVGL+ ++PT++ A+TN+LPA TF+LA +FR
Sbjct: 61  KTRPKITLRILVQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVTFLLAAIFR 120

Query: 121 QESVRIKTKPGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMER-NPNRR 180
           QE+V IK   G AKV GT+VCV GAM+LSFYHGHTIG+GESKIHW Y E + +  + +  
Sbjct: 121 QETVGIKKASGQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENITKHGSSSGH 180

Query: 181 ENRILGSILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERNI 240
            N  LG  L++ ++ +WA WF+IQ ++S  F APYTST L+C M   +CG +ALI +  I
Sbjct: 181 SNFFLGPFLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQCGAIALISDHTI 240

Query: 241 AAWSLKSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWALL 300
           + WSL S +R ++ LY GVV S L F + SW +QRKGPLYVS+F+PLLL++VAI SWALL
Sbjct: 241 SDWSLSSPLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWALL 300

Query: 301 HEQLYVGTVIGSVLIIIGLYAVLWGKSKEMNKLQDHNMENPTMIEKPNGNISHNIDEK 358
            E+LY GT +GS L++IGLY VLWGK +E+++ ++   +      K     + +I+ +
Sbjct: 301 EEKLYTGTFMGSALVVIGLYGVLWGKDREVSEKEEEREKVKQQNHKVKSESNEDIESR 358

BLAST of Tan0010383 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 257.7 bits (657), Expect = 1.4e-68
Identity = 139/324 (42.90%), Postives = 215/324 (66.36%), Query Frame = 0

Query: 12  MILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTERKTRPKITFPIL 71
           MI LQ  Y G+NII+K+++ +GM+ +VL+ YR    T  IAPFAFF ERK +PKITF I 
Sbjct: 22  MISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKITFSIF 81

Query: 72  FQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFRQESVRIKTKPG 131
            Q+ +  L G   +Q F+++GLKYT+PT S AM+N+LPA TFILAVLFR E + +K    
Sbjct: 82  MQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLWC 141

Query: 132 FAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMERNPNRRENR------ILG 191
            AK+AGT+V V GAML++ Y G  + L  +K  + +I+     N    +N       + G
Sbjct: 142 QAKIAGTVVTVAGAMLMTIYKGPIVELFWTK--YMHIQDSSHANTTSSKNSSSDKEFLKG 201

Query: 192 SILLLCSSFAWALWFVIQARLSAKF-KAPYTSTTLLCFMAFFECGFVALILERNIAAWSL 251
           SILL+ ++ AWA  FV+QA++   + K   + TTL+CF+   +   V  ++E N +AW +
Sbjct: 202 SILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAWRI 261

Query: 252 KSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWALLHEQLY 311
              + L+A  Y+G+V S +++ +   +++++GP++ + F+PL+++IVA++   +L E+++
Sbjct: 262 GWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIF 321

Query: 312 VGTVIGSVLIIIGLYAVLWGKSKE 329
           +G VIG+VLI+IGLYAVLWGK KE
Sbjct: 322 LGGVIGAVLIVIGLYAVLWGKQKE 343

BLAST of Tan0010383 vs. TAIR 10
Match: AT3G30340.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.9e-65
Identity = 128/323 (39.63%), Postives = 206/323 (63.78%), Query Frame = 0

Query: 8   AMLGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTERKTRPKIT 67
           A+L M ++ I    +N++ K  +  G+N  V  TYR   GT  + PFA F ER  RPK+T
Sbjct: 11  AVLMMSMINIGLSVVNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLERHNRPKLT 70

Query: 68  FPILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFRQESVRIK 127
             IL  +   +L G +  Q FF +GL+YT+ T S A +N++P+ TF LA++FRQE++ IK
Sbjct: 71  GRILCSLFFSALLGTSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFRQETLNIK 130

Query: 128 TKPGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMERNPN--RRENRILG 187
           +  G AK+ GT++C+CGA++L+ Y G  +    S+ H  ++E     +      +   +G
Sbjct: 131 SNVGRAKLLGTMICICGALVLTLYKGTAL----SREHSTHMETHTRTDSTGAMTQKWAMG 190

Query: 188 SILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERNIAAWSLK 247
           SI+L+ S   W+ WF++QA++S  +   YTSTT+L F    +   ++LI ER+ + W +K
Sbjct: 191 SIMLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMWVVK 250

Query: 248 STIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWALLHEQLYV 307
              +++A+LY+G+V SGL +   SW ++++G ++ S F PL+ +  AI S++ LHEQ+Y 
Sbjct: 251 DKFQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQIYC 310

Query: 308 GTVIGSVLIIIGLYAVLWGKSKE 329
           G+VIGS++II+GLY +LWGKSK+
Sbjct: 311 GSVIGSMVIIVGLYILLWGKSKD 329

BLAST of Tan0010383 vs. TAIR 10
Match: AT5G13670.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.3e-61
Identity = 132/335 (39.40%), Postives = 202/335 (60.30%), Query Frame = 0

Query: 10  LGMILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTERKTRPKITFP 69
           + ++ +Q  Y  ++I++KLA+  GM+P VL+ YR    +  I PFA   ER TRPK+TF 
Sbjct: 10  IAIVFIQCLYALMSIVAKLALNKGMSPHVLVAYRMAVASALITPFALILERNTRPKLTFK 69

Query: 70  ILFQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFRQESVRIKTK 129
           IL QI + SL      Q  ++ G+K T  T +SA+ N LPA TFI+A +F+ E V I+ +
Sbjct: 70  ILLQIAILSLFEPVVEQNLYYSGMKLTTATFTSALCNALPAMTFIMACVFKLEKVTIERR 129

Query: 130 PGFAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMERNPNRRENRI------ 189
              AK+ GT+V + GAML++F  G+ I L       P+       N +    RI      
Sbjct: 130 HSQAKLVGTMVAIGGAMLMTFVKGNVIEL-------PWTSNSRGLNGHTHAMRIPKQADI 189

Query: 190 -LGSILLLCSSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILER-NIAA 249
             GSI+L+ S F+W+ + ++QA++ A++KA  + T L+C M   E   + LI ER N++ 
Sbjct: 190 ARGSIMLVASCFSWSCYIILQAKILAQYKAELSLTALMCIMGMLEATVMGLIWERKNMSV 249

Query: 250 WSLKSTIRLVAVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWALLHE 309
           W +   + L+A +Y G+V SGL + +  W  + +GP++VS F PL +++VAILS  +  E
Sbjct: 250 WKINPDVTLLASIYGGLV-SGLAYYVIGWASKERGPVFVSAFNPLSMVLVAILSTFVFLE 309

Query: 310 QLYVGTVIGSVLIIIGLYAVLWGKSKEMNKLQDHN 337
           ++YVG VIGSV+I+IG+Y VLWGKSK+   +   N
Sbjct: 310 KVYVGRVIGSVVIVIGIYLVLWGKSKDKGGMLQPN 336

BLAST of Tan0010383 vs. TAIR 10
Match: AT2G40900.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 232.6 bits (592), Expect = 4.9e-61
Identity = 128/356 (35.96%), Postives = 218/356 (61.24%), Query Frame = 0

Query: 12  MILLQICYGGLNIISKLAMQSGMNPFVLLTYRQIFGTFAIAPFAFFTERKTRPKITFPIL 71
           M+ LQ  Y G+N+++K  +  GM+ +VL+ YR  F T AIAPFA  +ERK R K+TFPI 
Sbjct: 15  MVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVRSKMTFPIF 74

Query: 72  FQILLCSLSGATANQIFFFVGLKYTNPTVSSAMTNVLPAATFILAVLFRQESVRIKTKPG 131
            +I L +L G   +Q  +++GLK T+PT SSA++N++PA T ILA LFR E V ++    
Sbjct: 75  MRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEKVEMRKVRC 134

Query: 132 FAKVAGTIVCVCGAMLLSFYHGHTIGLGESKIHWPYIERMMERNPNRRENRILGSILLLC 191
             KV GT+V V G++L+ FY G  I    S         +   +     + +  ++ LL 
Sbjct: 135 LVKVMGTLVTVVGSILMIFYKGPFINFFRS--------HLTAASSPPTADYLKAAVFLLL 194

Query: 192 SSFAWALWFVIQARLSAKFKAPYTSTTLLCFMAFFECGFVALILERNIAAWSLKSTIRLV 251
           +S +WA +FV+QA    K+ A  + +T++CFM   +   +A ++E N +A ++   + L+
Sbjct: 195 ASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNPSALNIGFDMNLL 254

Query: 252 AVLYTGVVCSGLTFSITSWIIQRKGPLYVSIFTPLLLIIVAILSWALLHEQLYVGTVIGS 311
           A  Y G++ S + + +   ++QRKGP++V+ F PL+++IV+I+S+ +L + +Y+G VIG 
Sbjct: 255 ASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGIYLGGVIGV 314

Query: 312 VLIIIGLYAVLWGKSKEMNKLQDHNMENPTMIEKPNGN----ISHNIDEKNDMELQ 364
           V++++G+YAVLWGK  + +  +  + +N   ++  +GN    I   IDE ++ +++
Sbjct: 315 VVLMVGVYAVLWGKHVDDDGEETRHEDNVVAVKCCSGNNGLTIMPKIDEADEEDVE 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GXB42.4e-10551.96WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2... [more]
Q9FL412.0e-6742.90WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2... [more]
Q9LI652.7e-6439.63WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2... [more]
Q9FNA51.8e-6039.40WAT1-related protein At5g13670 OS=Arabidopsis thaliana OX=3702 GN=At5g13670 PE=2... [more]
F4IJ086.9e-6035.96WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2... [more]
Match NameE-valueIdentityDescription
XP_022156429.11.6e-16784.07WAT1-related protein At1g09380-like [Momordica charantia][more]
XP_038891245.11.8e-16183.57WAT1-related protein At1g09380 [Benincasa hispida][more]
XP_008453225.21.1e-15880.91PREDICTED: WAT1-related protein At1g09380 [Cucumis melo] >KAA0048114.1 WAT1-rela... [more]
XP_022946709.16.9e-15879.19WAT1-related protein At1g09380 [Cucurbita moschata][more]
KAG7030108.12.0e-15778.92WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1DTF98.0e-16884.07WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111023321 PE=3 SV=1[more]
A0A5A7TYK55.2e-15980.91WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385... [more]
A0A1S3BWI15.2e-15980.91WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103494009 PE=3 SV=1[more]
A0A6J1G4M73.4e-15879.19WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111450701 PE=3 SV=1[more]
A0A6J1KB701.1e-15678.11WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111493889 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G09380.11.7e-10651.96nodulin MtN21 /EamA-like transporter family protein [more]
AT5G07050.11.4e-6842.90nodulin MtN21 /EamA-like transporter family protein [more]
AT3G30340.11.9e-6539.63nodulin MtN21 /EamA-like transporter family protein [more]
AT5G13670.11.3e-6139.40nodulin MtN21 /EamA-like transporter family protein [more]
AT2G40900.14.9e-6135.96nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 184..322
e-value: 1.1E-16
score: 61.2
coord: 8..149
e-value: 7.8E-16
score: 58.5
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 7..336
NoneNo IPR availablePANTHERPTHR31218:SF326WAT1-RELATED PROTEINcoord: 7..336
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 42..152
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 242..327

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0010383.1Tan0010383.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity