Homology
BLAST of Tan0010379 vs. ExPASy Swiss-Prot
Match:
Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)
HSP 1 Score: 422.2 bits (1084), Expect = 6.6e-117
Identity = 197/338 (58.28%), Postives = 249/338 (73.67%), Query Frame = 0
Query: 39 ESAVPENFIWPDEFKASDSVPELLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVV 98
+S +P FIWPDE K S +PEL VP IDL ++ EA R++ EAC KHGFF+V
Sbjct: 39 QSQIPNQFIWPDEEKPSIDIPELNVPFIDL-----SSQDSTLEAPRVIAEACTKHGFFLV 98
Query: 99 VNHGVDMELMKDVHECMDEFFTLPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFS 158
VNHGV L+ D H M+ FF +PL KQKAQRK GE+ GYA+SF GRFS+KLPWKET S
Sbjct: 99 VNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLS 158
Query: 159 LRCLAHHQNFSTAHDFVSQSLGQQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREI 218
+ + T D+ S +LGQ+F GKVY + + +S L +KI+ELL LSLG+ R+
Sbjct: 159 FQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDY 218
Query: 219 FKKFYEDNDSILRLNYYPRCEKPQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSI 278
F+ F+E+NDSI+RLN+YP C+ P LTLGTGPHCDP+S+TILHQDHV+GLQV+VD+QW SI
Sbjct: 219 FRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSI 278
Query: 279 PPTPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELV 338
P P +FVVNIGDTFM+L+NG++KSC HRAVVN + ARKSMAFFLCP DKVV+ P +++
Sbjct: 279 RPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDIL 338
Query: 339 EMYPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTAWM 377
E RK+PD+TW M LE TQK YRAD NT +F+ W+
Sbjct: 339 EKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371
BLAST of Tan0010379 vs. ExPASy Swiss-Prot
Match:
Q39112 (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1)
HSP 1 Score: 413.7 bits (1062), Expect = 2.4e-114
Identity = 198/342 (57.89%), Postives = 245/342 (71.64%), Query Frame = 0
Query: 42 VPENFIWPDEFKASDSVPELLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVVVNH 101
+P+ F+WPD K S V L VP IDL FLSG+ EATRLV +A KHGFF++ NH
Sbjct: 37 IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96
Query: 102 GVDMELMKDVHECMDEFFTLPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFSLRC 161
GVD L+ + MD FF P KQKAQRK GE+ GYA+SF+GRFSSKLPWKET S +
Sbjct: 97 GVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKF 156
Query: 162 LAHHQNFS-TAHDFVSQSLGQQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREIFK 221
+ S T DFVS+ +G + GKVY E + ++ L +KI+ELL +SLG+ R FK
Sbjct: 157 SPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFK 216
Query: 222 KFYEDNDSILRLNYYPRCEKPQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPP 281
+F+ED+DSI RLNYYP+C++P+L LGTGPHCDPTS+TILHQD V GLQV+VD++W SIPP
Sbjct: 217 EFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPP 276
Query: 282 TPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELVE- 341
P +FVVNIGDTFM+LTNG YKSC HRAVVN + RK+ AFFLCP G+KVV+ PEELV
Sbjct: 277 NPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNG 336
Query: 342 -MYPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTAWMQQAK 381
RK+PD+TW M LE TQK YRAD NT F+ W++ +
Sbjct: 337 VKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRR 378
BLAST of Tan0010379 vs. ExPASy Swiss-Prot
Match:
Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)
HSP 1 Score: 411.8 bits (1057), Expect = 8.9e-114
Identity = 193/338 (57.10%), Postives = 245/338 (72.49%), Query Frame = 0
Query: 39 ESAVPENFIWPDEFKASDSVPELLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVV 98
++ +P FIWPD+ K S +V EL VP IDL+ LS + +A+RL+ EAC+KHGFF+V
Sbjct: 37 QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLV 96
Query: 99 VNHGVDMELMKDVHECMDEFFTLPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFS 158
VNHG+ EL+ D HE FF +PL KQ+ RK GE+ GYA+SF GRFS+KLPWKET S
Sbjct: 97 VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156
Query: 159 LRCLAHHQNFSTAHDFVSQSLGQQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREI 218
R + D+ +LG F GKVY E + +S L +KI+ELL LSLG+ R+
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216
Query: 219 FKKFYEDNDSILRLNYYPRCEKPQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSI 278
F++F+E+NDSI+RLNYYP C KP LTLGTGPHCDPTS+TILHQDHV+GLQV+V++QW SI
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276
Query: 279 PPTPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELV 338
P P +FVVNIGDTFM+L+N YKSC HRAVVN + RKS+AFFLCP D+VV P EL+
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336
Query: 339 EMYPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTAWM 377
+ R++PD+TW M LE TQK YRAD NT +AF+ W+
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373
BLAST of Tan0010379 vs. ExPASy Swiss-Prot
Match:
Q9C955 (Gibberellin 20 oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GA20OX4 PE=2 SV=1)
HSP 1 Score: 402.9 bits (1034), Expect = 4.2e-111
Identity = 193/339 (56.93%), Postives = 243/339 (71.68%), Query Frame = 0
Query: 42 VPENFIWPDEFKASDSVPELLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVVVNH 101
+P+ F+WPD K S +VP L VP IDL FLS + V EA RLV EA +KHGFF+V NH
Sbjct: 36 IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95
Query: 102 GVDMELMKDVHECMDEFFTLPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKET--FSL 161
GVD L+ H+ MD FF P K KAQRK+GE GYA+SF+GRF LPWKET FS
Sbjct: 96 GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155
Query: 162 RCLAHHQNFS-TAHDFVSQSLGQQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREI 221
+N+S T +++S+++G + G VY E +T+S+L +KI+ELL +SLG+ RE
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215
Query: 222 FKKFYEDNDSILRLNYYPRCEKPQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSI 281
F++F+EDN+SI RLNYYP+C++P L LGTGPHCDPTS+TIL QD VSGLQV+VD+QW SI
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275
Query: 282 PPTPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELV 341
PP P + VVNIGDT M+LTNG+YKSC HRAVVN + RK++AFFLCP DKVV+ P EL
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL- 335
Query: 342 EMYPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTAWMQ 378
R +PD+TW M LE T K YRAD NT + FT W++
Sbjct: 336 --EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWLK 371
BLAST of Tan0010379 vs. ExPASy Swiss-Prot
Match:
O04706 (Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1)
HSP 1 Score: 391.7 bits (1005), Expect = 9.6e-108
Identity = 188/346 (54.34%), Postives = 243/346 (70.23%), Query Frame = 0
Query: 37 SGESAVPENFIWPD-EFKASDSVPELLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGF 96
SG + +P FIWP+ E D+ EL VP ID+ LSG+ R E TRLV EAC +HGF
Sbjct: 12 SGRADIPSQFIWPEGESPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACERHGF 71
Query: 97 FVVVNHGVDMELMKDVHECMDEFFTLPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKE 156
F VVNHG+D EL+ D H C+D FFT+PL KQ+A R+ GE+ GYA+SF GRF+SKLPWKE
Sbjct: 72 FQVVNHGIDAELLADAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKE 131
Query: 157 TFSLRCLAHHQNFSTAHDFVSQSLGQQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLP 216
T S R + + D++ +LG+ G+VY +S L ++I+E+L SLG+
Sbjct: 132 TLSFRSCP--SDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVG 191
Query: 217 REIFKKFYEDNDSILRLNYYPRCEKPQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQW 276
R +++F+E NDSI+RLNYYP C++P TLGTGPHCDPTS+TILHQD+V GLQV+ + +W
Sbjct: 192 RAHYRRFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRW 251
Query: 277 HSIPPTPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPE 336
SI P D+FVVNIGDTFM+L+NG YKSC HRAVVN K RKS+AFFLCP DKVV P
Sbjct: 252 RSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLCPEMDKVVAPPG 311
Query: 337 ELVEMYPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTAWMQQAKK 382
LV+ PR +PD+TW LL+ TQK YRAD T + F++W+ Q ++
Sbjct: 312 TLVDAANPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQQQQ 355
BLAST of Tan0010379 vs. NCBI nr
Match:
CAA70329.1 (gibberellin 20-oxidase [Marah macrocarpa])
HSP 1 Score: 652.1 bits (1681), Expect = 3.0e-183
Identity = 314/388 (80.93%), Postives = 344/388 (88.66%), Query Frame = 0
Query: 1 MAIKCMMAAESSPSSSISAEESKEMMKREHRVAFGESGESAVPENFIWPDEFKASDSVPE 60
MAI CMMA ESSP+ E +KREH+VAFG +GES+VPENFIWPDEFKA+ PE
Sbjct: 1 MAITCMMATESSPA---------EEVKREHQVAFGGAGESSVPENFIWPDEFKATADAPE 60
Query: 61 LLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVVVNHGVDMELMKDVHECMDEFFT 120
L VPHIDLKK LSG+E+DVEEATRLVDEACRKHGFFVVVNHGVD ELM VHECM+EFFT
Sbjct: 61 LHVPHIDLKKVLSGDEKDVEEATRLVDEACRKHGFFVVVNHGVDKELMNKVHECMNEFFT 120
Query: 121 LPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFSLRCLAHHQNFSTAHDFVSQSLG 180
LPLDVKQKA RK+GEN+GYANSF+GRFS+KLPWKETFSLR LA H+N STA D+VSQ LG
Sbjct: 121 LPLDVKQKAHRKVGENFGYANSFIGRFSTKLPWKETFSLRYLA-HENSSTARDYVSQVLG 180
Query: 181 QQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREIFKKFYEDNDSILRLNYYPRCEK 240
+FSHHG+VY ECGK LSDL ++IVELL LSLG+ RE F+KFYEDNDSI+R+NYYPRCEK
Sbjct: 181 PEFSHHGEVYQECGKALSDLSLRIVELLGLSLGISRETFRKFYEDNDSIMRMNYYPRCEK 240
Query: 241 PQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPTPDSFVVNIGDTFMSLTNGV 300
P+LTLGTGPHCDPTSITILHQD VSGLQVYVDDQWHSIPPT DSFVVN+GDTFMSLTNGV
Sbjct: 241 PELTLGTGPHCDPTSITILHQDDVSGLQVYVDDQWHSIPPTEDSFVVNVGDTFMSLTNGV 300
Query: 301 YKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELVEMYPPRKFPDYTWPMLLEMTQK 360
YKSCFHRAVVNCKEARKSMAFFLCPA +KVVRAPEELVE YPPRKFPDYTWPMLLEMTQK
Sbjct: 301 YKSCFHRAVVNCKEARKSMAFFLCPAVEKVVRAPEELVEKYPPRKFPDYTWPMLLEMTQK 360
Query: 361 FYRADSNTFKAFTAWMQQAKKLDTADSP 389
+YRADSNTF AFT W+QQ + LD+ SP
Sbjct: 361 YYRADSNTFNAFTTWIQQ-QNLDSTASP 377
BLAST of Tan0010379 vs. NCBI nr
Match:
XP_023552793.1 (gibberellin 20 oxidase 2-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 642.9 bits (1657), Expect = 1.8e-180
Identity = 306/393 (77.86%), Postives = 340/393 (86.51%), Query Frame = 0
Query: 1 MAIKCMMAAESSPSSSISAEESKEMMKREHRVAFGESGESAVPENFIWPDEFKASDSVPE 60
MAIKCM A+S S + K+ MKR+HRV FG S ES VPENFIWP+EFKA +SVPE
Sbjct: 1 MAIKCMTTAKSQGESKL-----KQEMKRKHRVEFGSSDESMVPENFIWPEEFKARESVPE 60
Query: 61 LLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVVVNHGVDMELMKDVHECMDEFFT 120
L VPHIDL+KF+S NE D+EE RLVDEACRKHGFFV+VNHGVDMELMK+VHECMDEFF+
Sbjct: 61 LQVPHIDLQKFMSENENDIEEVRRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFS 120
Query: 121 LPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFSLRCLAHHQNFSTAHDFVSQSLG 180
LPLDVKQKAQRK+GENYGYANSF+GRFSSKLPWKETFSLR AHH NF HD+V LG
Sbjct: 121 LPLDVKQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHH-NFPITHDYVCHHLG 180
Query: 181 QQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREIFKKFYEDNDSILRLNYYPRCEK 240
Q+FS GKVY ECGK LSDL +KIVELL LSLGLP++ FKKF+EDNDSI+RLNYYP C K
Sbjct: 181 QEFSQQGKVYEECGKALSDLSLKIVELLGLSLGLPKQKFKKFFEDNDSIIRLNYYPPCNK 240
Query: 241 PQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPTPDSFVVNIGDTFMSLTNGV 300
P+LTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPP DSFV+NIGDTFM+LTNGV
Sbjct: 241 PELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGV 300
Query: 301 YKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELVEMYPPRKFPDYTWPMLLEMTQK 360
YKSCFHRAVVNC+EARKS+ FFLCPAGDKVVRAP+E+V+ PPRKFPDYTWPMLLE+TQK
Sbjct: 301 YKSCFHRAVVNCREARKSIVFFLCPAGDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQK 360
Query: 361 FYRADSNTFKAFTAWMQQAKKLDTADSPIAPSV 394
FYR+DSNTFKAFT W+Q K L T S +APS+
Sbjct: 361 FYRSDSNTFKAFTTWLQH-KNLATTASSLAPSL 386
BLAST of Tan0010379 vs. NCBI nr
Match:
XP_022984437.1 (gibberellin 20 oxidase 2-like [Cucurbita maxima])
HSP 1 Score: 641.7 bits (1654), Expect = 4.1e-180
Identity = 307/393 (78.12%), Postives = 340/393 (86.51%), Query Frame = 0
Query: 1 MAIKCMMAAESSPSSSISAEESKEMMKREHRVAFGESGESAVPENFIWPDEFKASDSVPE 60
MAIKCM A+S S + K+ MKR+HRV FG S ES VPENFIWPDEFKA +SVPE
Sbjct: 1 MAIKCMTTAKSQGESKL-----KQEMKRKHRVEFGSSDESMVPENFIWPDEFKARESVPE 60
Query: 61 LLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVVVNHGVDMELMKDVHECMDEFFT 120
L VPHIDL+KF+S NE D+EEATRLVDEACRKHGFFV+VNHGVDMELMK+VHECMDEFF+
Sbjct: 61 LDVPHIDLQKFVSENENDIEEATRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFS 120
Query: 121 LPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFSLRCLAHHQNFSTAHDFVSQSLG 180
LPLDVKQKAQRK+GENYGYANSF+GRFSSKLPWKETFSLR AHH N HD+V LG
Sbjct: 121 LPLDVKQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHH-NLPVTHDYVCHHLG 180
Query: 181 QQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREIFKKFYEDNDSILRLNYYPRCEK 240
+FS GKVY ECGK LSDL +KIVELL LSLG+P+E FKKF+EDNDSI+RLNYYP C K
Sbjct: 181 PEFSQQGKVYEECGKALSDLSLKIVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNK 240
Query: 241 PQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPTPDSFVVNIGDTFMSLTNGV 300
P+LTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPP DSFV+NIGDTFM+LTNGV
Sbjct: 241 PELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGV 300
Query: 301 YKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELVEMYPPRKFPDYTWPMLLEMTQK 360
YKSCFHRAVVNC+EARKS+ FFLCPA DKVVRAP+E+V+ PPRKFPDYTWPMLLE+TQK
Sbjct: 301 YKSCFHRAVVNCREARKSIVFFLCPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQK 360
Query: 361 FYRADSNTFKAFTAWMQQAKKLDTADSPIAPSV 394
FYR+DSNTFKAFT W+Q K L TA S +APS+
Sbjct: 361 FYRSDSNTFKAFTTWLQH-KNLATAASSLAPSL 386
BLAST of Tan0010379 vs. NCBI nr
Match:
CBL59326.1 (Gibberellin 20-oxidase [Cucurbita maxima])
HSP 1 Score: 640.2 bits (1650), Expect = 1.2e-179
Identity = 306/393 (77.86%), Postives = 340/393 (86.51%), Query Frame = 0
Query: 1 MAIKCMMAAESSPSSSISAEESKEMMKREHRVAFGESGESAVPENFIWPDEFKASDSVPE 60
MAIKCM A+S S + K+ MKR+HRV FG S ES VPENFIWPDEFKA +SVPE
Sbjct: 1 MAIKCMTTAKSQGESKL-----KQEMKRKHRVEFGSSDESMVPENFIWPDEFKARESVPE 60
Query: 61 LLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVVVNHGVDMELMKDVHECMDEFFT 120
L VPHIDL+KF+S NE D+EEATRLVDEACRKHGFFV+VNHGVDMELMK+VHECMDEFF+
Sbjct: 61 LDVPHIDLQKFVSENENDIEEATRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFS 120
Query: 121 LPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFSLRCLAHHQNFSTAHDFVSQSLG 180
LPLDVKQKAQRK+GENYGYANSF+GRFSSKLPWKETFSLR AHH N HD+V LG
Sbjct: 121 LPLDVKQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHH-NLPVTHDYVCHHLG 180
Query: 181 QQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREIFKKFYEDNDSILRLNYYPRCEK 240
+FS GKVY ECG+ LSDL +KIVELL LSLG+P+E FKKF+EDNDSI+RLNYYP C K
Sbjct: 181 PEFSQQGKVYEECGEALSDLSLKIVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNK 240
Query: 241 PQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPTPDSFVVNIGDTFMSLTNGV 300
P+LTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPP DSFV+NIGDTFM+LTNGV
Sbjct: 241 PELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGV 300
Query: 301 YKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELVEMYPPRKFPDYTWPMLLEMTQK 360
YKSCFHRAVVNC+EARKS+ FFLCPA DKVVRAP+E+V+ PPRKFPDYTWPMLLE+TQK
Sbjct: 301 YKSCFHRAVVNCREARKSIVFFLCPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQK 360
Query: 361 FYRADSNTFKAFTAWMQQAKKLDTADSPIAPSV 394
FYR+DSNTFKAFT W+Q K L TA S +APS+
Sbjct: 361 FYRSDSNTFKAFTTWLQH-KNLATAASSLAPSL 386
BLAST of Tan0010379 vs. NCBI nr
Match:
XP_022922616.1 (gibberellin 20 oxidase 2-like [Cucurbita moschata] >KAG6576758.1 Gibberellin 20 oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 639.8 bits (1649), Expect = 1.5e-179
Identity = 305/393 (77.61%), Postives = 340/393 (86.51%), Query Frame = 0
Query: 1 MAIKCMMAAESSPSSSISAEESKEMMKREHRVAFGESGESAVPENFIWPDEFKASDSVPE 60
MAIKCM A+S S + K+ MKR+HRV FG S ES VPENFIWP+EFKA +SVPE
Sbjct: 1 MAIKCMTTAKSQGESKL-----KQEMKRKHRVEFGSSDESMVPENFIWPEEFKARESVPE 60
Query: 61 LLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVVVNHGVDMELMKDVHECMDEFFT 120
L VPHIDL+KF+S NE D+EE RLVDEACRKHGFFV+VNHGVDMELMK+VHECMDEFF+
Sbjct: 61 LHVPHIDLQKFMSENENDIEEVRRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFS 120
Query: 121 LPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFSLRCLAHHQNFSTAHDFVSQSLG 180
LPLDVKQKAQRK+GENYGYANSF+GRFSSKLPWKETFSLR AHH HD+V LG
Sbjct: 121 LPLDVKQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHH---PITHDYVCHHLG 180
Query: 181 QQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREIFKKFYEDNDSILRLNYYPRCEK 240
Q+FS GKVY ECGK LSDL +KIVELL LSLG+P++ FKKF+EDNDSI+RLNYYP C K
Sbjct: 181 QEFSQQGKVYEECGKALSDLSLKIVELLGLSLGVPKQKFKKFFEDNDSIIRLNYYPPCNK 240
Query: 241 PQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPTPDSFVVNIGDTFMSLTNGV 300
P+LTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPP DSFV+NIGDTFM+LTNGV
Sbjct: 241 PELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGV 300
Query: 301 YKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELVEMYPPRKFPDYTWPMLLEMTQK 360
YKSCFHRAVVNC+EARKS+ FFLCPAGDKVVRAP+E+V+ PPRKFPDYTWPMLLE+TQK
Sbjct: 301 YKSCFHRAVVNCREARKSIVFFLCPAGDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQK 360
Query: 361 FYRADSNTFKAFTAWMQQAKKLDTADSPIAPSV 394
FYR+DSNTFKAFT W+Q KKL TA S +APS+
Sbjct: 361 FYRSDSNTFKAFTTWLQH-KKLATAASSLAPSL 384
BLAST of Tan0010379 vs. ExPASy TrEMBL
Match:
Q96498 (Gibberellin 20-oxidase OS=Marah macrocarpa OX=48242 PE=2 SV=1)
HSP 1 Score: 652.1 bits (1681), Expect = 1.5e-183
Identity = 314/388 (80.93%), Postives = 344/388 (88.66%), Query Frame = 0
Query: 1 MAIKCMMAAESSPSSSISAEESKEMMKREHRVAFGESGESAVPENFIWPDEFKASDSVPE 60
MAI CMMA ESSP+ E +KREH+VAFG +GES+VPENFIWPDEFKA+ PE
Sbjct: 1 MAITCMMATESSPA---------EEVKREHQVAFGGAGESSVPENFIWPDEFKATADAPE 60
Query: 61 LLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVVVNHGVDMELMKDVHECMDEFFT 120
L VPHIDLKK LSG+E+DVEEATRLVDEACRKHGFFVVVNHGVD ELM VHECM+EFFT
Sbjct: 61 LHVPHIDLKKVLSGDEKDVEEATRLVDEACRKHGFFVVVNHGVDKELMNKVHECMNEFFT 120
Query: 121 LPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFSLRCLAHHQNFSTAHDFVSQSLG 180
LPLDVKQKA RK+GEN+GYANSF+GRFS+KLPWKETFSLR LA H+N STA D+VSQ LG
Sbjct: 121 LPLDVKQKAHRKVGENFGYANSFIGRFSTKLPWKETFSLRYLA-HENSSTARDYVSQVLG 180
Query: 181 QQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREIFKKFYEDNDSILRLNYYPRCEK 240
+FSHHG+VY ECGK LSDL ++IVELL LSLG+ RE F+KFYEDNDSI+R+NYYPRCEK
Sbjct: 181 PEFSHHGEVYQECGKALSDLSLRIVELLGLSLGISRETFRKFYEDNDSIMRMNYYPRCEK 240
Query: 241 PQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPTPDSFVVNIGDTFMSLTNGV 300
P+LTLGTGPHCDPTSITILHQD VSGLQVYVDDQWHSIPPT DSFVVN+GDTFMSLTNGV
Sbjct: 241 PELTLGTGPHCDPTSITILHQDDVSGLQVYVDDQWHSIPPTEDSFVVNVGDTFMSLTNGV 300
Query: 301 YKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELVEMYPPRKFPDYTWPMLLEMTQK 360
YKSCFHRAVVNCKEARKSMAFFLCPA +KVVRAPEELVE YPPRKFPDYTWPMLLEMTQK
Sbjct: 301 YKSCFHRAVVNCKEARKSMAFFLCPAVEKVVRAPEELVEKYPPRKFPDYTWPMLLEMTQK 360
Query: 361 FYRADSNTFKAFTAWMQQAKKLDTADSP 389
+YRADSNTF AFT W+QQ + LD+ SP
Sbjct: 361 YYRADSNTFNAFTTWIQQ-QNLDSTASP 377
BLAST of Tan0010379 vs. ExPASy TrEMBL
Match:
A0A6J1J263 (gibberellin 20 oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482738 PE=3 SV=1)
HSP 1 Score: 641.7 bits (1654), Expect = 2.0e-180
Identity = 307/393 (78.12%), Postives = 340/393 (86.51%), Query Frame = 0
Query: 1 MAIKCMMAAESSPSSSISAEESKEMMKREHRVAFGESGESAVPENFIWPDEFKASDSVPE 60
MAIKCM A+S S + K+ MKR+HRV FG S ES VPENFIWPDEFKA +SVPE
Sbjct: 1 MAIKCMTTAKSQGESKL-----KQEMKRKHRVEFGSSDESMVPENFIWPDEFKARESVPE 60
Query: 61 LLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVVVNHGVDMELMKDVHECMDEFFT 120
L VPHIDL+KF+S NE D+EEATRLVDEACRKHGFFV+VNHGVDMELMK+VHECMDEFF+
Sbjct: 61 LDVPHIDLQKFVSENENDIEEATRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFS 120
Query: 121 LPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFSLRCLAHHQNFSTAHDFVSQSLG 180
LPLDVKQKAQRK+GENYGYANSF+GRFSSKLPWKETFSLR AHH N HD+V LG
Sbjct: 121 LPLDVKQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHH-NLPVTHDYVCHHLG 180
Query: 181 QQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREIFKKFYEDNDSILRLNYYPRCEK 240
+FS GKVY ECGK LSDL +KIVELL LSLG+P+E FKKF+EDNDSI+RLNYYP C K
Sbjct: 181 PEFSQQGKVYEECGKALSDLSLKIVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNK 240
Query: 241 PQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPTPDSFVVNIGDTFMSLTNGV 300
P+LTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPP DSFV+NIGDTFM+LTNGV
Sbjct: 241 PELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGV 300
Query: 301 YKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELVEMYPPRKFPDYTWPMLLEMTQK 360
YKSCFHRAVVNC+EARKS+ FFLCPA DKVVRAP+E+V+ PPRKFPDYTWPMLLE+TQK
Sbjct: 301 YKSCFHRAVVNCREARKSIVFFLCPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQK 360
Query: 361 FYRADSNTFKAFTAWMQQAKKLDTADSPIAPSV 394
FYR+DSNTFKAFT W+Q K L TA S +APS+
Sbjct: 361 FYRSDSNTFKAFTTWLQH-KNLATAASSLAPSL 386
BLAST of Tan0010379 vs. ExPASy TrEMBL
Match:
I0J1I7 (Gibberellin 20-oxidase OS=Cucurbita maxima OX=3661 GN=ga20ox4 PE=2 SV=1)
HSP 1 Score: 640.2 bits (1650), Expect = 5.7e-180
Identity = 306/393 (77.86%), Postives = 340/393 (86.51%), Query Frame = 0
Query: 1 MAIKCMMAAESSPSSSISAEESKEMMKREHRVAFGESGESAVPENFIWPDEFKASDSVPE 60
MAIKCM A+S S + K+ MKR+HRV FG S ES VPENFIWPDEFKA +SVPE
Sbjct: 1 MAIKCMTTAKSQGESKL-----KQEMKRKHRVEFGSSDESMVPENFIWPDEFKARESVPE 60
Query: 61 LLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVVVNHGVDMELMKDVHECMDEFFT 120
L VPHIDL+KF+S NE D+EEATRLVDEACRKHGFFV+VNHGVDMELMK+VHECMDEFF+
Sbjct: 61 LDVPHIDLQKFVSENENDIEEATRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFS 120
Query: 121 LPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFSLRCLAHHQNFSTAHDFVSQSLG 180
LPLDVKQKAQRK+GENYGYANSF+GRFSSKLPWKETFSLR AHH N HD+V LG
Sbjct: 121 LPLDVKQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHH-NLPVTHDYVCHHLG 180
Query: 181 QQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREIFKKFYEDNDSILRLNYYPRCEK 240
+FS GKVY ECG+ LSDL +KIVELL LSLG+P+E FKKF+EDNDSI+RLNYYP C K
Sbjct: 181 PEFSQQGKVYEECGEALSDLSLKIVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNK 240
Query: 241 PQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPTPDSFVVNIGDTFMSLTNGV 300
P+LTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPP DSFV+NIGDTFM+LTNGV
Sbjct: 241 PELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGV 300
Query: 301 YKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELVEMYPPRKFPDYTWPMLLEMTQK 360
YKSCFHRAVVNC+EARKS+ FFLCPA DKVVRAP+E+V+ PPRKFPDYTWPMLLE+TQK
Sbjct: 301 YKSCFHRAVVNCREARKSIVFFLCPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQK 360
Query: 361 FYRADSNTFKAFTAWMQQAKKLDTADSPIAPSV 394
FYR+DSNTFKAFT W+Q K L TA S +APS+
Sbjct: 361 FYRSDSNTFKAFTTWLQH-KNLATAASSLAPSL 386
BLAST of Tan0010379 vs. ExPASy TrEMBL
Match:
A0A6J1E4L1 (gibberellin 20 oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111430571 PE=3 SV=1)
HSP 1 Score: 639.8 bits (1649), Expect = 7.5e-180
Identity = 305/393 (77.61%), Postives = 340/393 (86.51%), Query Frame = 0
Query: 1 MAIKCMMAAESSPSSSISAEESKEMMKREHRVAFGESGESAVPENFIWPDEFKASDSVPE 60
MAIKCM A+S S + K+ MKR+HRV FG S ES VPENFIWP+EFKA +SVPE
Sbjct: 1 MAIKCMTTAKSQGESKL-----KQEMKRKHRVEFGSSDESMVPENFIWPEEFKARESVPE 60
Query: 61 LLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVVVNHGVDMELMKDVHECMDEFFT 120
L VPHIDL+KF+S NE D+EE RLVDEACRKHGFFV+VNHGVDMELMK+VHECMDEFF+
Sbjct: 61 LHVPHIDLQKFMSENENDIEEVRRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFS 120
Query: 121 LPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFSLRCLAHHQNFSTAHDFVSQSLG 180
LPLDVKQKAQRK+GENYGYANSF+GRFSSKLPWKETFSLR AHH HD+V LG
Sbjct: 121 LPLDVKQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHH---PITHDYVCHHLG 180
Query: 181 QQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREIFKKFYEDNDSILRLNYYPRCEK 240
Q+FS GKVY ECGK LSDL +KIVELL LSLG+P++ FKKF+EDNDSI+RLNYYP C K
Sbjct: 181 QEFSQQGKVYEECGKALSDLSLKIVELLGLSLGVPKQKFKKFFEDNDSIIRLNYYPPCNK 240
Query: 241 PQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPTPDSFVVNIGDTFMSLTNGV 300
P+LTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPP DSFV+NIGDTFM+LTNGV
Sbjct: 241 PELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGV 300
Query: 301 YKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELVEMYPPRKFPDYTWPMLLEMTQK 360
YKSCFHRAVVNC+EARKS+ FFLCPAGDKVVRAP+E+V+ PPRKFPDYTWPMLLE+TQK
Sbjct: 301 YKSCFHRAVVNCREARKSIVFFLCPAGDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQK 360
Query: 361 FYRADSNTFKAFTAWMQQAKKLDTADSPIAPSV 394
FYR+DSNTFKAFT W+Q KKL TA S +APS+
Sbjct: 361 FYRSDSNTFKAFTTWLQH-KKLATAASSLAPSL 384
BLAST of Tan0010379 vs. ExPASy TrEMBL
Match:
Q9XHR0 (Gibberellin 20-oxidase OS=Citrullus lanatus OX=3654 GN=WGA20OXI PE=2 SV=1)
HSP 1 Score: 623.6 bits (1607), Expect = 5.5e-175
Identity = 296/388 (76.29%), Postives = 329/388 (84.79%), Query Frame = 0
Query: 1 MAIKCMMAAESSPSSSISAEESKEMMKREHRVAFGESGESAVPENFIWPDEFKASDSVPE 60
MA+KCMM S ESKEM + HRV FG S ES VPENF+WPDEFKA D PE
Sbjct: 1 MAMKCMM----------STGESKEMKSKLHRVEFGCSEESMVPENFVWPDEFKAKDGAPE 60
Query: 61 LLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVVVNHGVDMELMKDVHECMDEFFT 120
L VPHIDL KFL+GNE D+EEATRLVDEACRKHGFFV+VNHGVDMEL+K VHECMDEFFT
Sbjct: 61 LHVPHIDLHKFLNGNESDIEEATRLVDEACRKHGFFVLVNHGVDMELIKGVHECMDEFFT 120
Query: 121 LPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFSLRCLAHHQNFSTAHDFVSQSLG 180
LP DVKQK+QRK GENYGYANSF+GRFS+ LPWKET SL +A+H N ++ DFVS+ +G
Sbjct: 121 LPFDVKQKSQRKFGENYGYANSFIGRFSNNLPWKETLSLPYVANH-NSTSVQDFVSKCVG 180
Query: 181 QQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREIFKKFYEDNDSILRLNYYPRCEK 240
+ SH GKVY ECGK LSDL +KIVELL LSLG+P+E FKKFYEDNDSI+RLNYYP CEK
Sbjct: 181 PELSHQGKVYQECGKALSDLSLKIVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEK 240
Query: 241 PQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPTPDSFVVNIGDTFMSLTNGV 300
P+LTLGTGPHCDPTSITILHQDHVSGLQVYVDD+WHSIPPT DSFV+NIGDTFM+LTNGV
Sbjct: 241 PELTLGTGPHCDPTSITILHQDHVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGV 300
Query: 301 YKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELVEMYPPRKFPDYTWPMLLEMTQK 360
YKSCFHRAVVNCKE RKSMAFFLCPA DKVVRAP+E+VE PPRKFPDYTWPMLLE+TQK
Sbjct: 301 YKSCFHRAVVNCKEVRKSMAFFLCPAADKVVRAPDEVVEKNPPRKFPDYTWPMLLELTQK 360
Query: 361 FYRADSNTFKAFTAWMQQAKKLDTADSP 389
FYRADSNTF+AF W+++ K +T P
Sbjct: 361 FYRADSNTFQAFIPWLEKQKSANTIAPP 377
BLAST of Tan0010379 vs. TAIR 10
Match:
AT5G51810.1 (gibberellin 20 oxidase 2 )
HSP 1 Score: 422.2 bits (1084), Expect = 4.7e-118
Identity = 197/338 (58.28%), Postives = 249/338 (73.67%), Query Frame = 0
Query: 39 ESAVPENFIWPDEFKASDSVPELLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVV 98
+S +P FIWPDE K S +PEL VP IDL ++ EA R++ EAC KHGFF+V
Sbjct: 39 QSQIPNQFIWPDEEKPSIDIPELNVPFIDL-----SSQDSTLEAPRVIAEACTKHGFFLV 98
Query: 99 VNHGVDMELMKDVHECMDEFFTLPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFS 158
VNHGV L+ D H M+ FF +PL KQKAQRK GE+ GYA+SF GRFS+KLPWKET S
Sbjct: 99 VNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLS 158
Query: 159 LRCLAHHQNFSTAHDFVSQSLGQQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREI 218
+ + T D+ S +LGQ+F GKVY + + +S L +KI+ELL LSLG+ R+
Sbjct: 159 FQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDY 218
Query: 219 FKKFYEDNDSILRLNYYPRCEKPQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSI 278
F+ F+E+NDSI+RLN+YP C+ P LTLGTGPHCDP+S+TILHQDHV+GLQV+VD+QW SI
Sbjct: 219 FRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSI 278
Query: 279 PPTPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELV 338
P P +FVVNIGDTFM+L+NG++KSC HRAVVN + ARKSMAFFLCP DKVV+ P +++
Sbjct: 279 RPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDIL 338
Query: 339 EMYPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTAWM 377
E RK+PD+TW M LE TQK YRAD NT +F+ W+
Sbjct: 339 EKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371
BLAST of Tan0010379 vs. TAIR 10
Match:
AT5G07200.1 (gibberellin 20-oxidase 3 )
HSP 1 Score: 413.7 bits (1062), Expect = 1.7e-115
Identity = 198/342 (57.89%), Postives = 245/342 (71.64%), Query Frame = 0
Query: 42 VPENFIWPDEFKASDSVPELLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVVVNH 101
+P+ F+WPD K S V L VP IDL FLSG+ EATRLV +A KHGFF++ NH
Sbjct: 37 IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96
Query: 102 GVDMELMKDVHECMDEFFTLPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFSLRC 161
GVD L+ + MD FF P KQKAQRK GE+ GYA+SF+GRFSSKLPWKET S +
Sbjct: 97 GVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKF 156
Query: 162 LAHHQNFS-TAHDFVSQSLGQQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREIFK 221
+ S T DFVS+ +G + GKVY E + ++ L +KI+ELL +SLG+ R FK
Sbjct: 157 SPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFK 216
Query: 222 KFYEDNDSILRLNYYPRCEKPQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPP 281
+F+ED+DSI RLNYYP+C++P+L LGTGPHCDPTS+TILHQD V GLQV+VD++W SIPP
Sbjct: 217 EFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPP 276
Query: 282 TPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELVE- 341
P +FVVNIGDTFM+LTNG YKSC HRAVVN + RK+ AFFLCP G+KVV+ PEELV
Sbjct: 277 NPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNG 336
Query: 342 -MYPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTAWMQQAK 381
RK+PD+TW M LE TQK YRAD NT F+ W++ +
Sbjct: 337 VKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRR 378
BLAST of Tan0010379 vs. TAIR 10
Match:
AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 411.8 bits (1057), Expect = 6.4e-115
Identity = 193/338 (57.10%), Postives = 245/338 (72.49%), Query Frame = 0
Query: 39 ESAVPENFIWPDEFKASDSVPELLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVV 98
++ +P FIWPD+ K S +V EL VP IDL+ LS + +A+RL+ EAC+KHGFF+V
Sbjct: 37 QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLV 96
Query: 99 VNHGVDMELMKDVHECMDEFFTLPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKETFS 158
VNHG+ EL+ D HE FF +PL KQ+ RK GE+ GYA+SF GRFS+KLPWKET S
Sbjct: 97 VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156
Query: 159 LRCLAHHQNFSTAHDFVSQSLGQQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREI 218
R + D+ +LG F GKVY E + +S L +KI+ELL LSLG+ R+
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216
Query: 219 FKKFYEDNDSILRLNYYPRCEKPQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSI 278
F++F+E+NDSI+RLNYYP C KP LTLGTGPHCDPTS+TILHQDHV+GLQV+V++QW SI
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276
Query: 279 PPTPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELV 338
P P +FVVNIGDTFM+L+N YKSC HRAVVN + RKS+AFFLCP D+VV P EL+
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336
Query: 339 EMYPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTAWM 377
+ R++PD+TW M LE TQK YRAD NT +AF+ W+
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373
BLAST of Tan0010379 vs. TAIR 10
Match:
AT1G60980.1 (gibberellin 20-oxidase 4 )
HSP 1 Score: 402.9 bits (1034), Expect = 3.0e-112
Identity = 193/339 (56.93%), Postives = 243/339 (71.68%), Query Frame = 0
Query: 42 VPENFIWPDEFKASDSVPELLVPHIDLKKFLSGNERDVEEATRLVDEACRKHGFFVVVNH 101
+P+ F+WPD K S +VP L VP IDL FLS + V EA RLV EA +KHGFF+V NH
Sbjct: 36 IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95
Query: 102 GVDMELMKDVHECMDEFFTLPLDVKQKAQRKLGENYGYANSFLGRFSSKLPWKET--FSL 161
GVD L+ H+ MD FF P K KAQRK+GE GYA+SF+GRF LPWKET FS
Sbjct: 96 GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155
Query: 162 RCLAHHQNFS-TAHDFVSQSLGQQFSHHGKVYHECGKTLSDLCMKIVELLALSLGLPREI 221
+N+S T +++S+++G + G VY E +T+S+L +KI+ELL +SLG+ RE
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215
Query: 222 FKKFYEDNDSILRLNYYPRCEKPQLTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSI 281
F++F+EDN+SI RLNYYP+C++P L LGTGPHCDPTS+TIL QD VSGLQV+VD+QW SI
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275
Query: 282 PPTPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNCKEARKSMAFFLCPAGDKVVRAPEELV 341
PP P + VVNIGDT M+LTNG+YKSC HRAVVN + RK++AFFLCP DKVV+ P EL
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL- 335
Query: 342 EMYPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTAWMQ 378
R +PD+TW M LE T K YRAD NT + FT W++
Sbjct: 336 --EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWLK 371
BLAST of Tan0010379 vs. TAIR 10
Match:
AT1G44090.1 (gibberellin 20-oxidase 5 )
HSP 1 Score: 285.0 bits (728), Expect = 9.0e-77
Identity = 149/362 (41.16%), Postives = 213/362 (58.84%), Query Frame = 0
Query: 17 ISAEESKEMMKREHRVAFGESGESAVPENFIWPDEFKASDSVPELLVPHIDLKKFLSGNE 76
+ +S+EM + + +S + VP F WP++ + S +L +P IDL FL+GNE
Sbjct: 19 VKRPKSREMNSSDVNFSLLQS-QPNVPAEFFWPEK-DVAPSEGDLDLPIIDLSGFLNGNE 78
Query: 77 RDVEEATRLVDEACRKHGFFVVVNHGVDMELMKDVHECMDEFFTLPLDVKQKAQRKLGEN 136
+ + A + V +AC HG F+VVNHG L + E FF L D K +A R G
Sbjct: 79 AETQLAAKAVKKACMAHGTFLVVNHGFKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNI 138
Query: 137 YGYANSFLGRFSSKLPWKETFSLRCLAHHQNFSTAHDFVSQSLGQQFSHHGKVYHECGKT 196
GY RFSS LPW ET +L + DF++ LG G+V+ E
Sbjct: 139 SGYTAGHSQRFSSNLPWNETLTLAFKKGPPH--VVEDFLTSRLGNHRQEIGQVFQEFCDA 198
Query: 197 LSDLCMKIVELLALSLGL-PREIFKKFYEDNDSILRLNYYPRCEKPQLTLGTGPHCDPTS 256
++ L M ++ELL +S+GL R +++F+ED I R NYYP C++P+ LG GPH DPT+
Sbjct: 199 MNGLVMDLMELLGISMGLKDRTYYRRFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTA 258
Query: 257 ITILHQDHVSGLQVYVDDQWHSIPPTPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNCKEA 316
IT+L QD V GL+V+ W ++ P P + VVN+GDTFM+L+NG Y+SC+HRAVVN ++
Sbjct: 259 ITVLLQDDVVGLEVFAAGSWQTVRPRPGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKV 318
Query: 317 RKSMAFFLCPAGDKVVRAPEELVE-MYPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTA 376
R+S+ FF CP DK++ P ELVE RK+PD+TW L + TQ YR D+ T F++
Sbjct: 319 RRSLVFFSCPREDKIIVPPPELVEGEEASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFSS 376
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q39111 | 6.6e-117 | 58.28 | Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1 | [more] |
Q39112 | 2.4e-114 | 57.89 | Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1 | [more] |
Q39110 | 8.9e-114 | 57.10 | Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2 | [more] |
Q9C955 | 4.2e-111 | 56.93 | Gibberellin 20 oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GA20OX4 PE=2 SV=1 | [more] |
O04706 | 9.6e-108 | 54.34 | Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
CAA70329.1 | 3.0e-183 | 80.93 | gibberellin 20-oxidase [Marah macrocarpa] | [more] |
XP_023552793.1 | 1.8e-180 | 77.86 | gibberellin 20 oxidase 2-like [Cucurbita pepo subsp. pepo] | [more] |
XP_022984437.1 | 4.1e-180 | 78.12 | gibberellin 20 oxidase 2-like [Cucurbita maxima] | [more] |
CBL59326.1 | 1.2e-179 | 77.86 | Gibberellin 20-oxidase [Cucurbita maxima] | [more] |
XP_022922616.1 | 1.5e-179 | 77.61 | gibberellin 20 oxidase 2-like [Cucurbita moschata] >KAG6576758.1 Gibberellin 20 ... | [more] |
Match Name | E-value | Identity | Description | |
Q96498 | 1.5e-183 | 80.93 | Gibberellin 20-oxidase OS=Marah macrocarpa OX=48242 PE=2 SV=1 | [more] |
A0A6J1J263 | 2.0e-180 | 78.12 | gibberellin 20 oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482738 PE=3 S... | [more] |
I0J1I7 | 5.7e-180 | 77.86 | Gibberellin 20-oxidase OS=Cucurbita maxima OX=3661 GN=ga20ox4 PE=2 SV=1 | [more] |
A0A6J1E4L1 | 7.5e-180 | 77.61 | gibberellin 20 oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111430571 PE=3... | [more] |
Q9XHR0 | 5.5e-175 | 76.29 | Gibberellin 20-oxidase OS=Citrullus lanatus OX=3654 GN=WGA20OXI PE=2 SV=1 | [more] |