Tan0010374 (gene) Snake gourd v1

Overview
NameTan0010374
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionsubtilisin-like protease SBT4.3
LocationLG07: 12500128 .. 12504056 (-)
RNA-Seq ExpressionTan0010374
SyntenyTan0010374
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAGAAAAATTTTCCATTCTTTTTTTCCTTCATCACTGCTCTTTTAGTCGCCATTTTCAGCTCTGCAAATGGATCAGAAAGAAAACTTCATATTGTGTACATGGGGTCCATCAAAAACAGGGCAGTCGCAAAATCAACCCATCATTTGAATCTTCTTCAATCGGTGATTGGAACTTCTTTCAATGCTGAAAGTTCATTCATTCGTAGCTATGGAAGGAGCTTCAATGGCTTTGCTGCAAAACTCACTGACGCTGAAACAGAGCAACTTGCCGCCATGGAAGGAGTGGTCTCTGTTTTCGAGAGCAAGGAACTCAAAACCCAAACGACAAGATCGTGGGACTATGTGGGGTTCGGTGAAAATCCCAAAAGAAATCTCGCCGGTGAAGCCGACGTCATAATCGGGTCAATCGACACCGGAATTTGGCCGGAATCGGAGAGCTTCAACGACGAGGGAATAGGGCCACCACCGCCGCGGTGGAGAGGGGCGTGTGCCGGCGGAGAGAATTTCACGTGCAACAATAAGGTGATCGGAGCTCGGTTTTACTCCGCCGAGTCGGCGAGAGACAACGTCGGGCACGGCTCTCACACAGCCGCCACGGCGGCCGGAAAGTCGGTGAGAACGGGCGGATTTTATGGGATTGCCGGGGGAGTTGCGAGAGGAGCAGTTCCTTCTTCGAGAATCGCCGTTTACAAAGTGTGCAATCCGAGTTGCTCGGAAGTGGATATTTTGGCGGCGTTTGACGACGCCATTGCCGACGGTGTGGATGTTATTACGATTTCAATCGGCGGGCTGGGTGCGTTTGGCTTTAAATCGGACTCCATTGCAATTGGGTCTTTTCACGCAATGGCTAGAGGAATCCTGACCGTCCAATCCGCCGGAAATGATGGACCGGTGGAAGGATCGGTGGGAAGTGTAGCTCCGTGGCTGTTTACCGTTGCAGCCACCACTACAGATAGAACCATTGTTGACAAGGTTTCATCACTCAACTCAAAACCTTATTTCAAATCCTATTTTTTAGTAGTTTAAAAGTTGAACTTTTTTTTTTCCCCCTTTTAATTTCACCAACATTTGGGTCTCTTCAGTTCAGAGTACGGGTCAATTAGCGCTCAAACGTTAAACTTTTTAATTTAGGTTAAATAGGTGTCTAAATTTTAAAAAATATATAATAAGTTTGTAAACTTTGAAGTTTGTAAATAAGTCCTTATTTTTTAAAGGAGATAAGTTTTCATACACTCAATTTTGTGCTATGTTTAATACGTCATTACCCATTCGACATGTTTTAAAATTTATGAACTTATTGAACACAAAAAATAAAAGGTTAATGTCCTTAGACACAAAATTTTATGTTCAATAGATATGTTAATTTTAAAGAATGTTGAATATGTGAAAGATCTATTAGACACAAAATTAAAAGTTTAGAGATCTAGTAACATTTTTAGATGTTCTTTAGGGCGTTGAGTGAGTTATAATAAAATGACTTATAATAGTCAGTCTATTATAGTAGAATTATATAATAATGTTATTTAGAATGTAGAATTGTTTAGTTTAGAGTTATAATAATTTGTGTTTGGGGTGTAAAATATTTCACATGTTATCATAACTTTTGCCTCAAATAAGCCCTTAAAGTTAGTCTTACTAGACAGAATTGAAAGCTTAAAGACCTATTTTGAAAGCTTAAAGACCTATTAGATAGACAACATGAAAGTTTGAGGATTAAACCAATGATTTAACTTAGTTTAAAATAGTTATTTTTAGCACTTCACTTTTAAAATAATATTTTAATTCATGTTATGCAAAATCTTAACATAAATATTAATTACAATGAAAATTCTATACTAAAAACAAAATGGTCATTATTTAAAAATATAGTCATTAAAATGGACAAACTTTGAACGTATAGAAGCGGATATATAATATAAACTATTTGGTTAAATTACAAGTTTAGTACTTGAACTTTGCGGTTTGTGTCCATTTAGTTTCTAAACTTTAAAAAGTGTCTAATAGATCTTTGAACATTCAAATTTGTATCTATTTGGTTTCTAAACTTCGGAATGTGTTTAATAAGTTCTCAAATTTTCAATTTTGTGTCCAATAGCTCACTGAATTTTCAATTTTGTGTCTAATAGGTCTTTGACTTAGTCGACGTTTTTTAAAATTCATGAACTTACTAGGCACAAAATTAAAAACTTAGAGTTCTATTAGACAAAAAAATCATATTTTTATATCTAATAGATCAATTAATTTTAAAAAAGTATAAATATGTTAAGGACCTATTAGATACAAATTTAAAAGTTCAAGAACTAATTAGACATTTTTAAAGTTCAAGGACTTAATAGATATACAATTGAAAGTTTACGGACCTATTTGACACTTTTGTTAGTTCAGTGACGAAATAGACACAAATCTCAAAATTCAGGGATTAAACTGTTAATTTGACCAAACTATTTTTAAATTTATATCCTCAACATTCATCTTTATAGGTTTGGAAATCAACAAAAATCCAACAAGCGACCCACCATCTCAATTGAGTTTTTTCTTTCTTTCTTTCTTGAATTTAAGATTTCGAGATATCAATCTGTAATTATAGTCTCCATTACTAAATTATTATTGAATTAACAATTTTAATTGAGTTATCATTTTGCTTAAGCGAATGTCTTACAATGAGTTTAATTCTTTTGATTTATATTTCTAACAAGGTTGTACTCGGCAATGGAAAAACTGTCACCGGCGATTCAGTGAACTCCTTCTCTCCAAACAAGAACGTTCCATTGATTTACGGATCAAACGCTTCAAAAATTTGTTCCTCCTTCGATTCAGAAATTTGTTCGGAAGGTTGCTTGGATCCAGAATTGGTGAAAGGGAAGATCGTTCAATGTATGGCCTTTAATGGCGCCTCCGAAGCTTTTAAAGCCGGAGCCGACGGAGCAATTGTTCTCAACGACAAAGCCGATAATGTTTCCTTCGTTTTTCCACTGCCTGCAATCGCTTTGAACATCGAAGATTTCACAAATGTCGCAAATTACGCAACCTCTGCAAAAAATCCCCATGTCAGAATCCTCAAAAGCGTCGCCATTAGAGATCCTTACGCTCCAATGGCGGCCCAATTCTCCAGTCGCGGCCCCAATCTCTTAATGCCGGAGATCATGAAGCCCGACATCGCCGCCCCCGGCGTCGAAATCCTCGCCGCCTTTTCTCCGATTGCATCCCCTTCTAACGATCTCGGAGACAAAAGATCCGTCGATTTCACGATAATGTCCGGCACTTCCATGTCTTGTCCCCACGTCGCCGGTCTGGCTGCTTATGTAAAGAGCTTCCATCCAAATTGGTCGCCGGCGGCGATTAAATCCGCCGTCATGACGACGGCGGACCAGATCATGAATACTGACGGAGTTCAAATTGGGGAGTTTCTTTACGGGTCGGGTCAAATCAATCCAAATCAAGCAATCGAACCCGGCCTTGTTTATGAAACTTCCGAAGAGGATTATTTGGATTTGCTGTGTGGGAATGGGTTTGATTCGGAGACGGTGAGGATAATTTCAGGGAACGAGAGTGTTTGTTCGAGAAATTCGAGGAAATTTCCGCCGAAGGATTTGAATTATCCGGCAATGGTGGCGCAGGTTTTGCCGATGAAGCCGTTTGTGGTGACGTTTGAGAGAACAGTGATGAATGTTGGGGTTGCAAATTCAACTTACAAATCGGAGTTTTCGCCGTTCTCGAAAGTTTATGTTCTGAAGTCGCCGGAAAAGCTTAAGGTAAGTGTTGAGCCTCAAGATCTGTGGTTTGGAGATTTGAATGAGAAGAAGAGTTTTGTAGTGACGGTGATCGGAGGGGCGATTCCGTCGGAGACTGTGATTTCTTCAGCATTGGTTTGGAAGGATGGGATTCATAAAGTTAGAAGTCCGATTGTTTTGAAAGTTGTATAG

mRNA sequence

ATGGCGAAGAAAAATTTTCCATTCTTTTTTTCCTTCATCACTGCTCTTTTAGTCGCCATTTTCAGCTCTGCAAATGGATCAGAAAGAAAACTTCATATTGTGTACATGGGGTCCATCAAAAACAGGGCAGTCGCAAAATCAACCCATCATTTGAATCTTCTTCAATCGGTGATTGGAACTTCTTTCAATGCTGAAAGTTCATTCATTCGTAGCTATGGAAGGAGCTTCAATGGCTTTGCTGCAAAACTCACTGACGCTGAAACAGAGCAACTTGCCGCCATGGAAGGAGTGGTCTCTGTTTTCGAGAGCAAGGAACTCAAAACCCAAACGACAAGATCGTGGGACTATGTGGGGTTCGGTGAAAATCCCAAAAGAAATCTCGCCGGTGAAGCCGACGTCATAATCGGGTCAATCGACACCGGAATTTGGCCGGAATCGGAGAGCTTCAACGACGAGGGAATAGGGCCACCACCGCCGCGGTGGAGAGGGGCGTGTGCCGGCGGAGAGAATTTCACGTGCAACAATAAGGTGATCGGAGCTCGGTTTTACTCCGCCGAGTCGGCGAGAGACAACGTCGGGCACGGCTCTCACACAGCCGCCACGGCGGCCGGAAAGTCGGTGAGAACGGGCGGATTTTATGGGATTGCCGGGGGAGTTGCGAGAGGAGCAGTTCCTTCTTCGAGAATCGCCGTTTACAAAGTGTGCAATCCGAGTTGCTCGGAAGTGGATATTTTGGCGGCGTTTGACGACGCCATTGCCGACGGTGTGGATGTTATTACGATTTCAATCGGCGGGCTGGGTGCGTTTGGCTTTAAATCGGACTCCATTGCAATTGGGTCTTTTCACGCAATGGCTAGAGGAATCCTGACCGTCCAATCCGCCGGAAATGATGGACCGGTGGAAGGATCGGTGGGAAGTGTAGCTCCGTGGCTGTTTACCGTTGCAGCCACCACTACAGATAGAACCATTGTTGACAAGGTTGTACTCGGCAATGGAAAAACTGTCACCGGCGATTCAGTGAACTCCTTCTCTCCAAACAAGAACGTTCCATTGATTTACGGATCAAACGCTTCAAAAATTTGTTCCTCCTTCGATTCAGAAATTTGTTCGGAAGGTTGCTTGGATCCAGAATTGGTGAAAGGGAAGATCGTTCAATGTATGGCCTTTAATGGCGCCTCCGAAGCTTTTAAAGCCGGAGCCGACGGAGCAATTGTTCTCAACGACAAAGCCGATAATGTTTCCTTCGTTTTTCCACTGCCTGCAATCGCTTTGAACATCGAAGATTTCACAAATGTCGCAAATTACGCAACCTCTGCAAAAAATCCCCATGTCAGAATCCTCAAAAGCGTCGCCATTAGAGATCCTTACGCTCCAATGGCGGCCCAATTCTCCAGTCGCGGCCCCAATCTCTTAATGCCGGAGATCATGAAGCCCGACATCGCCGCCCCCGGCGTCGAAATCCTCGCCGCCTTTTCTCCGATTGCATCCCCTTCTAACGATCTCGGAGACAAAAGATCCGTCGATTTCACGATAATGTCCGGCACTTCCATGTCTTGTCCCCACGTCGCCGGTCTGGCTGCTTATGTAAAGAGCTTCCATCCAAATTGGTCGCCGGCGGCGATTAAATCCGCCGTCATGACGACGTTTCTTTACGGGTCGGGTCAAATCAATCCAAATCAAGCAATCGAACCCGGCCTTGTTTATGAAACTTCCGAAGAGGATTATTTGGATTTGCTGTGTGGGAATGGGTTTGATTCGGAGACGGTGAGGATAATTTCAGGGAACGAGAGTGTTTGTTCGAGAAATTCGAGGAAATTTCCGCCGAAGGATTTGAATTATCCGGCAATGGTGGCGCAGGTTTTGCCGATGAAGCCGTTTGTGGTGACGTTTGAGAGAACAGTGATGAATGTTGGGGTTGCAAATTCAACTTACAAATCGGAGTTTTCGCCGTTCTCGAAAGTTTATGTTCTGAAGTCGCCGGAAAAGCTTAAGGTAAGTGTTGAGCCTCAAGATCTGTGGTTTGGAGATTTGAATGAGAAGAAGAGTTTTGTAGTGACGGTGATCGGAGGGGCGATTCCGTCGGAGACTGTGATTTCTTCAGCATTGGTTTGGAAGGATGGGATTCATAAAGTTAGAAGTCCGATTGTTTTGAAAGTTGTATAG

Coding sequence (CDS)

ATGGCGAAGAAAAATTTTCCATTCTTTTTTTCCTTCATCACTGCTCTTTTAGTCGCCATTTTCAGCTCTGCAAATGGATCAGAAAGAAAACTTCATATTGTGTACATGGGGTCCATCAAAAACAGGGCAGTCGCAAAATCAACCCATCATTTGAATCTTCTTCAATCGGTGATTGGAACTTCTTTCAATGCTGAAAGTTCATTCATTCGTAGCTATGGAAGGAGCTTCAATGGCTTTGCTGCAAAACTCACTGACGCTGAAACAGAGCAACTTGCCGCCATGGAAGGAGTGGTCTCTGTTTTCGAGAGCAAGGAACTCAAAACCCAAACGACAAGATCGTGGGACTATGTGGGGTTCGGTGAAAATCCCAAAAGAAATCTCGCCGGTGAAGCCGACGTCATAATCGGGTCAATCGACACCGGAATTTGGCCGGAATCGGAGAGCTTCAACGACGAGGGAATAGGGCCACCACCGCCGCGGTGGAGAGGGGCGTGTGCCGGCGGAGAGAATTTCACGTGCAACAATAAGGTGATCGGAGCTCGGTTTTACTCCGCCGAGTCGGCGAGAGACAACGTCGGGCACGGCTCTCACACAGCCGCCACGGCGGCCGGAAAGTCGGTGAGAACGGGCGGATTTTATGGGATTGCCGGGGGAGTTGCGAGAGGAGCAGTTCCTTCTTCGAGAATCGCCGTTTACAAAGTGTGCAATCCGAGTTGCTCGGAAGTGGATATTTTGGCGGCGTTTGACGACGCCATTGCCGACGGTGTGGATGTTATTACGATTTCAATCGGCGGGCTGGGTGCGTTTGGCTTTAAATCGGACTCCATTGCAATTGGGTCTTTTCACGCAATGGCTAGAGGAATCCTGACCGTCCAATCCGCCGGAAATGATGGACCGGTGGAAGGATCGGTGGGAAGTGTAGCTCCGTGGCTGTTTACCGTTGCAGCCACCACTACAGATAGAACCATTGTTGACAAGGTTGTACTCGGCAATGGAAAAACTGTCACCGGCGATTCAGTGAACTCCTTCTCTCCAAACAAGAACGTTCCATTGATTTACGGATCAAACGCTTCAAAAATTTGTTCCTCCTTCGATTCAGAAATTTGTTCGGAAGGTTGCTTGGATCCAGAATTGGTGAAAGGGAAGATCGTTCAATGTATGGCCTTTAATGGCGCCTCCGAAGCTTTTAAAGCCGGAGCCGACGGAGCAATTGTTCTCAACGACAAAGCCGATAATGTTTCCTTCGTTTTTCCACTGCCTGCAATCGCTTTGAACATCGAAGATTTCACAAATGTCGCAAATTACGCAACCTCTGCAAAAAATCCCCATGTCAGAATCCTCAAAAGCGTCGCCATTAGAGATCCTTACGCTCCAATGGCGGCCCAATTCTCCAGTCGCGGCCCCAATCTCTTAATGCCGGAGATCATGAAGCCCGACATCGCCGCCCCCGGCGTCGAAATCCTCGCCGCCTTTTCTCCGATTGCATCCCCTTCTAACGATCTCGGAGACAAAAGATCCGTCGATTTCACGATAATGTCCGGCACTTCCATGTCTTGTCCCCACGTCGCCGGTCTGGCTGCTTATGTAAAGAGCTTCCATCCAAATTGGTCGCCGGCGGCGATTAAATCCGCCGTCATGACGACGTTTCTTTACGGGTCGGGTCAAATCAATCCAAATCAAGCAATCGAACCCGGCCTTGTTTATGAAACTTCCGAAGAGGATTATTTGGATTTGCTGTGTGGGAATGGGTTTGATTCGGAGACGGTGAGGATAATTTCAGGGAACGAGAGTGTTTGTTCGAGAAATTCGAGGAAATTTCCGCCGAAGGATTTGAATTATCCGGCAATGGTGGCGCAGGTTTTGCCGATGAAGCCGTTTGTGGTGACGTTTGAGAGAACAGTGATGAATGTTGGGGTTGCAAATTCAACTTACAAATCGGAGTTTTCGCCGTTCTCGAAAGTTTATGTTCTGAAGTCGCCGGAAAAGCTTAAGGTAAGTGTTGAGCCTCAAGATCTGTGGTTTGGAGATTTGAATGAGAAGAAGAGTTTTGTAGTGACGGTGATCGGAGGGGCGATTCCGTCGGAGACTGTGATTTCTTCAGCATTGGTTTGGAAGGATGGGATTCATAAAGTTAGAAGTCCGATTGTTTTGAAAGTTGTATAG

Protein sequence

MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGTSFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFGENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCSEVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKICSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDIAAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAAIKSAVMTTFLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKDGIHKVRSPIVLKVV
Homology
BLAST of Tan0010374 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 1.2e-212
Identity = 395/735 (53.74%), Postives = 506/735 (68.84%), Query Frame = 0

Query: 1   MAKKNFPFFFSFITALLVAIFSSANGSE-RKLHIVYMGSIKNRAVAKSTHHLNLLQSVIG 60
           MAK + P +   +  +     S+ +  +   ++IVYMG++     +  +HHL++LQ ++G
Sbjct: 1   MAKLSTPLYLICLAFIFTRDVSANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVG 60

Query: 61  TSFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGF 120
           T   A    +RSY RSFNGFAA L+ AE+++L  M+ VVSVF SK  +  TTRSWD+VGF
Sbjct: 61  T-IAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGF 120

Query: 121 GENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIG 180
           GE  +R    E+DVI+G ID+GIWPESESF+DEG GPPP +W+G+C GG  F CNNK+IG
Sbjct: 121 GEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIG 180

Query: 181 ARFYS--AESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNP 240
           ARFY+  A+SARD  GHG+HTA+TAAG +V+   FYG+A G ARG VPS+RIA YKVC  
Sbjct: 181 ARFYNKFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFN 240

Query: 241 SCSEVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGND 300
            C++VDILAAFDDAIADGVDVI+ISI         + S+AIGSFHAM RGI+T  SAGN+
Sbjct: 241 RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNN 300

Query: 301 GPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPN-KNVPLIYGSN 360
           GP +GSV +V+PW+ TVAA+ TDR  +D+VVLGNGK +TG SVN+F+ N    P++YG N
Sbjct: 301 GPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQN 360

Query: 361 ASKICSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFV 420
            S+ CS   +  CS GC+D ELVKGKIV C  F G  EA+ AGA G IV N    + +FV
Sbjct: 361 VSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAFV 420

Query: 421 FPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIM 480
            P PA +L  ED+ ++ +Y  SA+ P   IL++  I D  AP    FSSRGP+ ++  ++
Sbjct: 421 VPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLL 480

Query: 481 KPDIAAPGVEILAAFSPIASPSNDLG--DKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHP 540
           KPD++APG+EILAAFSP+ASPS+ L   DKRSV +++MSGTSM+CPHVAG+AAYVKSFHP
Sbjct: 481 KPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHP 540

Query: 541 NWSPAAIKSAVMTT-------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCG 600
           +WSP+AIKSA+MTT             F YGSGQINP +A +PGLVYE   EDYL +LC 
Sbjct: 541 DWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCA 600

Query: 601 NGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVAN 660
            GFDS T+   SG    CS  +     KDLNYP M   V  + PF VTF+RTV NVG  N
Sbjct: 601 EGFDSTTLTTTSGQNVTCSERTE---VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPN 660

Query: 661 STYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSA 717
           STYK+   P         PE L++S+EP+ L FG L EKKSFVVT+ G  +   + +SS+
Sbjct: 661 STYKASVVPL-------QPE-LQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSS 720

BLAST of Tan0010374 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 1.1e-194
Identity = 376/738 (50.95%), Postives = 492/738 (66.67%), Query Frame = 0

Query: 11  SFITALLVAIFSSA--NGSERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESS 70
           S I ALLV  F+SA  +  +++++IVYMG++ +R      +HH ++LQ V G S + +  
Sbjct: 11  SCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGES-SIQDR 70

Query: 71  FIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGF--GENPKR 130
            +R+Y RSFNGFAA+LT++E E LA+M+ VVSVF SK L  QTT SW+++G   G+  KR
Sbjct: 71  LVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKR 130

Query: 131 NLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYS- 190
           N   E+D IIG ID+GI+PES+SF+ +G GPPP +W+G C GG NFTCNNK+IGAR+Y+ 
Sbjct: 131 NPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYYTP 190

Query: 191 -----AESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPS- 250
                 ESARDN GHGSHTA+ AAG +V+   FYG+  G  RG VP++RIAVYKVC+P  
Sbjct: 191 KLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGV 250

Query: 251 --CSEVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGN 310
             C+   ILAAFDDAIAD VD+IT+S+G      F+ D++AIG+FHAMA+GILTV  AGN
Sbjct: 251 IRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGN 310

Query: 311 DGPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPN-KNVPLIYGS 370
           +GP   ++ S+APWLFTVAA+  +R  + KVVLGNGKT+ G SVNSF  N K  PL+YG 
Sbjct: 311 NGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVYGK 370

Query: 371 NASKICSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSF 430
           +AS  C +  +  CS GCLD + VKGKIV C       EA   GA  +IV N   D  S 
Sbjct: 371 SASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNPYEDAAS- 430

Query: 431 VFPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEI 490
           VF  P   L+ +D+  V +Y  S KNP   +LKS  I +  AP+ A +SSRGPN L+ +I
Sbjct: 431 VFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDI 490

Query: 491 MKPDIAAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPN 550
           +KPDI APG EILAA+SP   PS    D R V +T++SGTSMSCPHVAG+AAY+K+FHP 
Sbjct: 491 LKPDITAPGSEILAAYSPYVPPSE--SDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPL 550

Query: 551 WSPAAIKSAVMTT----------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLL 610
           WSP+ I+SA+MTT                F YG+G ++P  AI PGLVYE ++ D++  L
Sbjct: 551 WSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFL 610

Query: 611 CGNGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGV 670
           CG  +  + +R+ISG+ S C++   K   ++LNYP+M AQV   KPF VTF RTV NVG 
Sbjct: 611 CGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGR 670

Query: 671 ANSTYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVIS 718
            N+TYK++        V+ S  KLKV V P  L    L EKKSF VTV G    +E ++S
Sbjct: 671 PNATYKAK--------VVGS--KLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVS 730

BLAST of Tan0010374 vs. ExPASy Swiss-Prot
Match: F4JA91 (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 4.2e-194
Identity = 379/738 (51.36%), Postives = 497/738 (67.34%), Query Frame = 0

Query: 11  SFITALLVAIFSSA--NGSERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESS 70
           S I ALLV  F+SA  +  +++ +IVYMG++  R      +HH ++LQ V G S + E  
Sbjct: 12  SCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGES-SIEDR 71

Query: 71  FIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFGEN--PKR 130
            +R+Y RSFNGFAA+LT +E E LA+M+ VVSVF +K+LK QTT SW+++G  E+   KR
Sbjct: 72  LVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRTKR 131

Query: 131 NLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYS- 190
           N   E+D IIG ID+GI+PES+SF+ +G GPPP +W+G C GG+NFT NNK+IGAR+Y+ 
Sbjct: 132 NTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYYTP 191

Query: 191 -----AESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNP-- 250
                 ESARD +GHGSHTA+TAAG +V+   FYG+  G ARG VP++RIAVYKVC+P  
Sbjct: 192 KLEGFPESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDPGV 251

Query: 251 -SCSEVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGN 310
             C+   ILAAFDDAIAD VD+ITISIGG  +  F+ D IAIG+FHAMA+GIL V SAGN
Sbjct: 252 DGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSAGN 311

Query: 311 DGPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPN-KNVPLIYGS 370
            GP   +V S+APW+FTVAA+ T+R  V KVVLGNGKTV G SVNSF  N K  PL+YG 
Sbjct: 312 SGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVYGK 371

Query: 371 NASKICSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSF 430
           +AS  C +  +  CS GCLD + VKGKIV C +     EA   GA  +IV + + D V+ 
Sbjct: 372 SASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVRSHRTD-VAS 431

Query: 431 VFPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEI 490
           +F  P   L  +D+  V +Y  S KNP   +LKS  I +  AP+ A + SRGPN ++P+I
Sbjct: 432 IFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIPDI 491

Query: 491 MKPDIAAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPN 550
           +KPDI APG EI+AA+SP A PS  + D R V +++ +GTSMSCPHVAG+AAY+KSFHP 
Sbjct: 492 LKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFHPR 551

Query: 551 WSPAAIKSAVMTT----------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLL 610
           WSP+ I+SA+MTT                F YG+G ++P  AI PGLVYE ++ D++  L
Sbjct: 552 WSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIAFL 611

Query: 611 CGNGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGV 670
           CG  + ++ +R+ISG+ S C++   K  P++LNYP+M AQV   KPF V F RTV NVG 
Sbjct: 612 CGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNVGR 671

Query: 671 ANSTYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVIS 718
            N+TYK++        V+ S  KLKV V P  L    L EKKSF VT  G    +E ++S
Sbjct: 672 PNATYKAK--------VVGS--KLKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAENLVS 731

BLAST of Tan0010374 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 2.0e-191
Identity = 370/731 (50.62%), Postives = 487/731 (66.62%), Query Frame = 0

Query: 11  SFITALLVAIFSSANG--SERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESS 70
           S ++ LLV   SS +    +++++IVYMGS+ +RA    ++ H+N+LQ V G S + E  
Sbjct: 9   SLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGES-SIEGR 68

Query: 71  FIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGF--GENPKR 130
            +RSY RSFNGFAA+LT++E E++A M GVVSVF +K+L+ QTT SWD++G   G   KR
Sbjct: 69  LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKR 128

Query: 131 NLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYSA 190
           N   E+D IIG ID+GI PES+SF+D+G GPPP +W+G C+GG+NFTCNNK+IGAR Y++
Sbjct: 129 NPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS 188

Query: 191 ESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPS-CSEVDI 250
           E  RD  GHG+HTA+TAAG +V    F+GI  G  RG VP+SR+A YKVC P+ CS   +
Sbjct: 189 EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEAL 248

Query: 251 LAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPVEGSV 310
           L+AFDDAIADGVD+ITISIG   A  F++D IAIG+FHAMA+G+LTV SAGN GP   SV
Sbjct: 249 LSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISV 308

Query: 311 GSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFS-PNKNVPLIYG-SNASKICS 370
             VAPW+ TVAA+TT+R  V KVVLGNGKT+ G SVN++    K+ PL+YG S AS  C 
Sbjct: 309 SGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACD 368

Query: 371 SFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLPAI 430
           +  + +C   C+D   VKGKI+ C    G       GA G I    K D V+F+ PLPA 
Sbjct: 369 AESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAVGLIYRTPKPD-VAFIHPLPAA 428

Query: 431 ALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDIAA 490
            L  EDF ++ +Y  S  +P   +LK+ AI +  +P+ A FSSRGPN +  +I+KPDI A
Sbjct: 429 GLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITA 488

Query: 491 PGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAAIK 550
           PGVEILAA+SP   PS D  D R V ++++SGTSMSCPHVAG+AAYVK+F+P WSP+ I+
Sbjct: 489 PGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQ 548

Query: 551 SAVMTT---------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDSE 610
           SA+MTT               F YGSG ++P  A  PGLVYE  + D++  LCG  + S+
Sbjct: 549 SAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQ 608

Query: 611 TVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPM-KPFVVTFERTVMNVGVANSTYKS 670
            +++ISG    CS  ++K  P++LNYP+M A++      F VTF RT+ NVG  NSTY S
Sbjct: 609 VLKVISGETVTCS-EAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTS 668

Query: 671 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 718
           +        V     KL V + P  L F  +NEK+SF VTV G  + SE   S+ L+W D
Sbjct: 669 K-------VVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSD 727

BLAST of Tan0010374 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 2.5e-191
Identity = 370/736 (50.27%), Postives = 497/736 (67.53%), Query Frame = 0

Query: 10  FSFITALLVAIFSSANGSERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESSF 69
           +S++  LL++  S+    + +++IVYMGS+ +RA    ++ H+++LQ V G S + E   
Sbjct: 11  YSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGES-SIEGRL 70

Query: 70  IRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGF--GENPKRN 129
           +RSY RSFNGFAA+LT++E   +A +EGVVSVF +K L+  TT SWD++G   G+N KRN
Sbjct: 71  VRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRN 130

Query: 130 LAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYSAE 189
           LA E+D IIG IDTGIWPES+SF+D+G GPPP +W+G C+GG+NFTCNNK+IGAR Y++E
Sbjct: 131 LAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSE 190

Query: 190 SARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPS-CSEVDIL 249
             RD  GHG+HTA+TAAG +V+   F+GI  G  RG VP+SRIA YKVC  S CS   +L
Sbjct: 191 GTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALL 250

Query: 250 AAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPVEGSVG 309
           ++FDDAIADGVD+ITISIG      F+ D IAIG+FHAMA+GILTV SAGN GP   +V 
Sbjct: 251 SSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVS 310

Query: 310 SVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFS-PNKNVPLIYG-SNASKICSS 369
            VAPW+FTVAA+TT+R  + KVVLGNGKT+ G SVN+F    K  PL+YG S AS  C +
Sbjct: 311 HVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDA 370

Query: 370 FDSEICSEGCLDPELVKGKIVQCMAFNGASEAFK-AGADGAIVLNDKA--DNVSFVFPLP 429
             + +C+  CL+   VKGKI+ C    G    +K A + GAI + DK+   +V+F   LP
Sbjct: 371 KTAALCAPACLNKSRVKGKILVC----GGPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLP 430

Query: 430 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 489
           A  L  +DF ++ +Y  S  +P   +LK+  I +  +P+ A FSSRGPN +  +I+KPDI
Sbjct: 431 ASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDI 490

Query: 490 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 549
            APGVEILAAFSP   PS D  D R V +++ SGTSM+CPHVAG+AAYVK+F+P WSP+ 
Sbjct: 491 TAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSM 550

Query: 550 IKSAVMTT---------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFD 609
           I+SA+MTT               F YG+G ++P  A+ PGLVYE  + D++  LCG  + 
Sbjct: 551 IQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYT 610

Query: 610 SETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPM-KPFVVTFERTVMNVGVANSTY 669
           S+T++IISG+   CS+ + K  P++LNYP+M A++      F VTF RT+ NVG  NSTY
Sbjct: 611 SKTLKIISGDTVKCSKKN-KILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTY 670

Query: 670 KSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVW 721
           KS+        V     KL + V P  L+F  +NEK+SF VTV G  + SE   S+ L+W
Sbjct: 671 KSK-------VVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIW 730

BLAST of Tan0010374 vs. NCBI nr
Match: KAG6605933.1 (Subtilisin-like protease 4.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1070.5 bits (2767), Expect = 6.6e-309
Identity = 548/733 (74.76%), Postives = 620/733 (84.58%), Query Frame = 0

Query: 1   MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
           MA  NFP FFSFI ALL AIF+SANGSERK HIVYMG+I+NRA+A+STHHLNLL+SVIGT
Sbjct: 1   MAANNFPIFFSFIAALLAAIFTSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGT 60

Query: 61  SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
           S   E S+IRSYGRSFNGF A+LT  E EQLAAM+GVVSVFESKELKT+TTRSWD++GF 
Sbjct: 61  SSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSWDFLGF- 120

Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
           + PKRNLAGE DVIIGSID+GIWPESESFNDEGIGPPP +WRG CAGGENFTCN+KVIGA
Sbjct: 121 QQPKRNLAGELDVIIGSIDSGIWPESESFNDEGIGPPPQKWRGVCAGGENFTCNHKVIGA 180

Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
           R+YS+ SARD+ GHG+HTA+TA GKSV T GFYGIAGGVARGAVPSSR+AVY  C+PSC+
Sbjct: 181 RYYSSSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSPSCT 240

Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
           +V+ILAAFDDAIADGVD+ITIS+GG      + D IAIG++HAM +GILTVQSAGNDGPV
Sbjct: 241 DVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQSAGNDGPV 300

Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
            GSV SVAPWLF+VAATTTDR+IVD++VLGNGKTV+G S+N F+PNK+VPLIYG+NASK 
Sbjct: 301 VGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDVPLIYGTNASKS 360

Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
           CS  + E+CS  CLDP+LVKGKIVQC +F G  +A  AGA GAIVLNDKADNVSF+ PLP
Sbjct: 361 CSFQNPEVCS--CLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADNVSFIVPLP 420

Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
           AIALN   +  V NYA S  NPHVRILKSVAI+D YAPMAAQFSSRGPNLL+PEIMKPDI
Sbjct: 421 AIALNFTAYEQVTNYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLLPEIMKPDI 480

Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
           AAPGVEILA+ +P    S+  GD R+V+FTIMSGTSM+CPHVAGLAAYVKSFHPNWSP+A
Sbjct: 481 AAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPSA 540

Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
           IKSA+MTT              FL+GSGQINP QAIEPGLVYE  E+DYL  LCGNGFDS
Sbjct: 541 IKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKFLCGNGFDS 600

Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
           ++VR ISGN+S CS+ S KF P+DLNYPAMV +V PMKPFVV F+R V NVG ANSTYKS
Sbjct: 601 KSVRGISGNKSDCSKFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRIVTNVGTANSTYKS 660

Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
           EFSPFS VYVLKS EKL VSVEP +L F DLNEKKSFVVTV GG+IP+ T  SSAL+W D
Sbjct: 661 EFSPFSIVYVLKSSEKLNVSVEPTELTFKDLNEKKSFVVTVAGGSIPARTGFSSALIWSD 720

BLAST of Tan0010374 vs. NCBI nr
Match: XP_022995031.1 (subtilisin-like protease SBT4.3 [Cucurbita maxima])

HSP 1 Score: 1066.6 bits (2757), Expect = 9.3e-308
Identity = 542/733 (73.94%), Postives = 618/733 (84.31%), Query Frame = 0

Query: 1   MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
           MA  NFP  FSFI ALL AI SSANGSERK HIVYMG+I+NRA+A+STHHLNLL+SVIGT
Sbjct: 1   MAPDNFPILFSFIAALLAAILSSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGT 60

Query: 61  SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
           S   E S+IRSYGRSFNGF AKLT AE EQLAAM+GVVSVFESK  KTQTTRSWDY+GF 
Sbjct: 61  SSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFP 120

Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
             P RNLAGE DVIIGSIDTGIWPE ESFNDEGIGPPP RWRG CAGG NFTCNNKVIGA
Sbjct: 121 AKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGA 180

Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
           RFY++ SARDNVGHGSHTA+TAAGK  +T GFYG+AGG ARGAVPSSR+AVYKVCNP C 
Sbjct: 181 RFYTSMSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNPDCL 240

Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
           E +ILAAFDDAIADGVD+ITISIGG+G F F+ DS+AIGS+H+MA+GILTVQSAGN GPV
Sbjct: 241 EENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPV 300

Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
           +G+VGSV PW+FTVAATTTDRTIVDKVVLG+G TV G SVN+F+PN+NVPLIY +NAS+ 
Sbjct: 301 DGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNVPLIYATNASRN 360

Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
           CSS ++EICS+GCLDP LVKGKIVQC AF+GAS AF AGA GAIVLND A NVSFV P P
Sbjct: 361 CSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFP 420

Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
           AIAL + D+ +VANYA SA NP+V I +SVA +DPYAP  A FSSRGPN+ M EI+KPDI
Sbjct: 421 AIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILKPDI 480

Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
           AAPGVEILA+FSPIA+PS ++GDKRSV+F+I+SGTSMSCPHVAG+AAYVKSFHPNWSPAA
Sbjct: 481 AAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAA 540

Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
           IKSA+MTT              FLYGSG I+PN+AIEPGLVYE  E+D+L++LC  G+DS
Sbjct: 541 IKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDS 600

Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
           +T+   +GN+SVCS+   KF  +DLNYPAMVA VLP KPFVV F+RTV NVGVANSTY+S
Sbjct: 601 KTMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNVGVANSTYRS 660

Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
           +   FS V VLKS EKL VSV+PQ+LWF  LNEKKSFVVTV+GGAI +E V SSAL+W D
Sbjct: 661 KILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSD 720

BLAST of Tan0010374 vs. NCBI nr
Match: XP_023533940.1 (subtilisin-like protease SBT4.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1064.7 bits (2752), Expect = 3.5e-307
Identity = 544/733 (74.22%), Postives = 621/733 (84.72%), Query Frame = 0

Query: 1   MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
           MA  NFP  FSFI ALL AIF+SANGSERK HIVYMG+I+NRA+A+STHHLNLLQSVIGT
Sbjct: 1   MAANNFPISFSFIAALLAAIFTSANGSERKDHIVYMGAIQNRAMAESTHHLNLLQSVIGT 60

Query: 61  SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
           S   E S+IRSYGRSFNGF A+LT  E EQLAAM+GVVSVFESKELKT+TTRSWD++GF 
Sbjct: 61  SSVTEDSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSWDFLGF- 120

Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
           + PKRNLAGE DVIIGSID+GIWPESESFNDEG+GPPP +WRG CAGGENFTCN+KVIGA
Sbjct: 121 QQPKRNLAGELDVIIGSIDSGIWPESESFNDEGMGPPPQKWRGECAGGENFTCNHKVIGA 180

Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
           R+YS+ SARD+ GHG+HTA+TA GKSV T GFYGIAGGVARGAVPSSR+AVY  C+PSC+
Sbjct: 181 RYYSSSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSPSCT 240

Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
           +V+ILAAFDDAIADGVD+ITIS+GG      + D IAIG++HAM +GILTVQSAGNDGPV
Sbjct: 241 DVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQSAGNDGPV 300

Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
            GSV SVAPWLF+VAATTTDR+IVD++VLGNGKTV+G S+N F+PNK+VPLIYG+NASK 
Sbjct: 301 VGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDVPLIYGTNASKS 360

Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
           CS  + E+CS  CLDP+LVKGKIVQC +F G  +A  AGA GAIVLNDKADNVSF+ PLP
Sbjct: 361 CSFQNPEVCS--CLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADNVSFIVPLP 420

Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
           AIALN+  +  VANYA S  NPHVRIL+SVA++D YAPMAAQFSSRGPNLL+PEIMKPDI
Sbjct: 421 AIALNLTAYEQVANYAISEPNPHVRILQSVAVKDGYAPMAAQFSSRGPNLLLPEIMKPDI 480

Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
           AAPGVEILA+ +P    S+  GD R+V+FTIMSGTSM+CPHVAGLAAYVKSFHPNWSP+A
Sbjct: 481 AAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPSA 540

Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
           IKSA+MTT              FL+GSGQI+P QAIEPGLVYE  E+DYL  LCGNGFDS
Sbjct: 541 IKSAIMTTAEEMTNTDGFLSGEFLHGSGQIDPKQAIEPGLVYEIFEDDYLKFLCGNGFDS 600

Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
           ++VR ISGN+S CS+ S KF P+DLNYPAMV +V PMKPF V F+RTV NVG+ANSTYKS
Sbjct: 601 KSVRGISGNKSDCSKFSTKFSPQDLNYPAMVFEVPPMKPFAVKFQRTVTNVGIANSTYKS 660

Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
           EFSP S VYVLKS EKL VSVEP +L F DLNEKKSFVVTV GG+IP+ T  SSAL+W D
Sbjct: 661 EFSPLSVVYVLKSSEKLNVSVEPPELTFRDLNEKKSFVVTVAGGSIPARTGFSSALIWSD 720

BLAST of Tan0010374 vs. NCBI nr
Match: XP_022958362.1 (subtilisin-like protease SBT4.3 [Cucurbita moschata])

HSP 1 Score: 1064.3 bits (2751), Expect = 4.6e-307
Identity = 545/733 (74.35%), Postives = 619/733 (84.45%), Query Frame = 0

Query: 1   MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
           MA  NFP  F+FI ALL AIF+SANGSERK HIVYMG+I+NRA+A+STHHLNLL+SVIGT
Sbjct: 1   MAAINFPILFTFIAALLAAIFTSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGT 60

Query: 61  SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
           S   E S+IRSYGRSFNGF A+LT  E EQLAAM+GVVSVFESKELKT+TTRSWD++GF 
Sbjct: 61  SSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSWDFLGF- 120

Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
           + PKRNL+GE DVIIGSID+GIWPESESFND+GIGPPP +WRG CAGGENFTCN+KVIGA
Sbjct: 121 QQPKRNLSGELDVIIGSIDSGIWPESESFNDDGIGPPPQKWRGVCAGGENFTCNHKVIGA 180

Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
           R+YS+ SARD+ GHG+HTA+TA GKSV T GFYGIAGGVARGAVPSSR+AVY  C+PSC+
Sbjct: 181 RYYSSSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSPSCT 240

Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
           +V+ILAAFDDAIADGVD+ITIS+GG      + D IAIG++HAM +GILTVQSAGNDGPV
Sbjct: 241 DVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQSAGNDGPV 300

Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
            GSV SVAPWLF+VAATTTDR+IVD++VLGNGKTV+G S+N F+PNK+VPLIYG+NASK 
Sbjct: 301 VGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDVPLIYGTNASKS 360

Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
           CS  + E+CS  CLDP+LVKGKIVQC +F G  +A  AGA GAIVLNDKADNVSF+ PLP
Sbjct: 361 CSFQNPEVCS--CLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADNVSFIVPLP 420

Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
           A ALN   +  VANYA S  NPHVRILKSVAI+D YAPMAAQFSSRGPNLL+PEIMKPDI
Sbjct: 421 ATALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLLPEIMKPDI 480

Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
           AAPGVEILA+ +P    S+  GD R+V+FTIMSGTSM+CPHVAGLAAYVKSFHPNWSP+A
Sbjct: 481 AAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPSA 540

Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
           IKSA+MTT              FL+GSGQINP QAIEPGLVYE  E+DYL  LCGNGFDS
Sbjct: 541 IKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKFLCGNGFDS 600

Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
           ++VR ISGN+S CSR S KF P+DLNYPAMV +V PMKPFVV F+RTV NVG ANSTYKS
Sbjct: 601 KSVRGISGNKSDCSRFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRTVTNVGTANSTYKS 660

Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
           EFSPFS VYVLKS EKL VSVEP +L F DLNEKKSFVVTV GG+IP+ T  S AL+W D
Sbjct: 661 EFSPFSIVYVLKSSEKLNVSVEPPELTFKDLNEKKSFVVTVAGGSIPARTGFSLALIWSD 720

BLAST of Tan0010374 vs. NCBI nr
Match: XP_023533978.1 (subtilisin-like protease SBT4.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1064.3 bits (2751), Expect = 4.6e-307
Identity = 539/733 (73.53%), Postives = 619/733 (84.45%), Query Frame = 0

Query: 1   MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
           MA  NFP  FSFI ALL AIF+SANGSERK HIVYMG+I+NRA+A+STHHLNLL+SVIG+
Sbjct: 1   MAPDNFPILFSFIAALLAAIFTSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGS 60

Query: 61  SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
           S   E S+IRSYGRSFNGF AKLT  E EQLAAM+GVVSVFESK  KTQTTRSWDY+GF 
Sbjct: 61  SSVMEGSYIRSYGRSFNGFVAKLTGGEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFP 120

Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
             P RNLAGE DVIIGSIDTGIWPE ESFNDEGIGPPP RWRG CAGG NFTCNNKVIGA
Sbjct: 121 AKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGA 180

Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
           RFY++ SARDNVGHGSHTA+TAAGKS +T GFYG+AGGVARGAVPSSR+AVYK CNP C 
Sbjct: 181 RFYTSMSARDNVGHGSHTASTAAGKSGKTTGFYGLAGGVARGAVPSSRLAVYKACNPDCL 240

Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
           + +ILAAFDDAIADGVD+ITISI G G F F+ DS+AIGS+H+MA+GILTVQSAGN GP 
Sbjct: 241 DENILAAFDDAIADGVDLITISIVGAGGFNFEQDSMAIGSYHSMAKGILTVQSAGNYGPE 300

Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
           +G+VGSV PW+FTVAAT TDRTIVDKVVLG+G TV G SVN+F+PN+NVPLIY +NAS+ 
Sbjct: 301 DGTVGSVVPWVFTVAATNTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNVPLIYATNASRN 360

Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
           CSS ++EIC +GCLDP LVKGKIVQC AF+GAS AF AGA G IVLNDKA NVSFV P P
Sbjct: 361 CSSKNAEICRDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGGIVLNDKAANVSFVLPFP 420

Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
           AIAL + D+ +VANYA SA NP+V I +SVA +DPYAP  A FSSRGPN+ M EI+KPDI
Sbjct: 421 AIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILKPDI 480

Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
           AAPGVEILA+FSPIA+PS ++GDKRSV+F+I+SGTSMSCPHVAG+AAYVKSFHPNWSPAA
Sbjct: 481 AAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAA 540

Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
           IKSA+MTT              FLYGSG I+PN+AIEPGLVYE  E+D+L++LC  G+DS
Sbjct: 541 IKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDS 600

Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
           +T++   GN+SVCS+ S K+  +DLNYPAMVA VLPMKPFVV F+RT+ NVGVANSTY+S
Sbjct: 601 KTMKAFVGNDSVCSKTSTKYLARDLNYPAMVAHVLPMKPFVVKFQRTLTNVGVANSTYRS 660

Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
           + S FS V VLKS EKL VSV+PQ+LWF +LNEKKSFVVTV GGAIP+E V+SSAL+W D
Sbjct: 661 KISLFSGVNVLKSLEKLNVSVKPQELWFRNLNEKKSFVVTVAGGAIPTEIVLSSALIWSD 720

BLAST of Tan0010374 vs. ExPASy TrEMBL
Match: A0A6J1K0V8 (subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490708 PE=3 SV=1)

HSP 1 Score: 1066.6 bits (2757), Expect = 4.5e-308
Identity = 542/733 (73.94%), Postives = 618/733 (84.31%), Query Frame = 0

Query: 1   MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
           MA  NFP  FSFI ALL AI SSANGSERK HIVYMG+I+NRA+A+STHHLNLL+SVIGT
Sbjct: 1   MAPDNFPILFSFIAALLAAILSSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGT 60

Query: 61  SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
           S   E S+IRSYGRSFNGF AKLT AE EQLAAM+GVVSVFESK  KTQTTRSWDY+GF 
Sbjct: 61  SSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFP 120

Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
             P RNLAGE DVIIGSIDTGIWPE ESFNDEGIGPPP RWRG CAGG NFTCNNKVIGA
Sbjct: 121 AKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGA 180

Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
           RFY++ SARDNVGHGSHTA+TAAGK  +T GFYG+AGG ARGAVPSSR+AVYKVCNP C 
Sbjct: 181 RFYTSMSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNPDCL 240

Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
           E +ILAAFDDAIADGVD+ITISIGG+G F F+ DS+AIGS+H+MA+GILTVQSAGN GPV
Sbjct: 241 EENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPV 300

Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
           +G+VGSV PW+FTVAATTTDRTIVDKVVLG+G TV G SVN+F+PN+NVPLIY +NAS+ 
Sbjct: 301 DGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNVPLIYATNASRN 360

Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
           CSS ++EICS+GCLDP LVKGKIVQC AF+GAS AF AGA GAIVLND A NVSFV P P
Sbjct: 361 CSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFP 420

Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
           AIAL + D+ +VANYA SA NP+V I +SVA +DPYAP  A FSSRGPN+ M EI+KPDI
Sbjct: 421 AIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILKPDI 480

Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
           AAPGVEILA+FSPIA+PS ++GDKRSV+F+I+SGTSMSCPHVAG+AAYVKSFHPNWSPAA
Sbjct: 481 AAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAA 540

Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
           IKSA+MTT              FLYGSG I+PN+AIEPGLVYE  E+D+L++LC  G+DS
Sbjct: 541 IKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDS 600

Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
           +T+   +GN+SVCS+   KF  +DLNYPAMVA VLP KPFVV F+RTV NVGVANSTY+S
Sbjct: 601 KTMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNVGVANSTYRS 660

Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
           +   FS V VLKS EKL VSV+PQ+LWF  LNEKKSFVVTV+GGAI +E V SSAL+W D
Sbjct: 661 KILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSD 720

BLAST of Tan0010374 vs. ExPASy TrEMBL
Match: A0A6J1H1M3 (subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459607 PE=3 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 2.2e-307
Identity = 545/733 (74.35%), Postives = 619/733 (84.45%), Query Frame = 0

Query: 1   MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
           MA  NFP  F+FI ALL AIF+SANGSERK HIVYMG+I+NRA+A+STHHLNLL+SVIGT
Sbjct: 1   MAAINFPILFTFIAALLAAIFTSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGT 60

Query: 61  SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
           S   E S+IRSYGRSFNGF A+LT  E EQLAAM+GVVSVFESKELKT+TTRSWD++GF 
Sbjct: 61  SSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSWDFLGF- 120

Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
           + PKRNL+GE DVIIGSID+GIWPESESFND+GIGPPP +WRG CAGGENFTCN+KVIGA
Sbjct: 121 QQPKRNLSGELDVIIGSIDSGIWPESESFNDDGIGPPPQKWRGVCAGGENFTCNHKVIGA 180

Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
           R+YS+ SARD+ GHG+HTA+TA GKSV T GFYGIAGGVARGAVPSSR+AVY  C+PSC+
Sbjct: 181 RYYSSSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSPSCT 240

Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
           +V+ILAAFDDAIADGVD+ITIS+GG      + D IAIG++HAM +GILTVQSAGNDGPV
Sbjct: 241 DVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQSAGNDGPV 300

Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
            GSV SVAPWLF+VAATTTDR+IVD++VLGNGKTV+G S+N F+PNK+VPLIYG+NASK 
Sbjct: 301 VGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDVPLIYGTNASKS 360

Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
           CS  + E+CS  CLDP+LVKGKIVQC +F G  +A  AGA GAIVLNDKADNVSF+ PLP
Sbjct: 361 CSFQNPEVCS--CLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADNVSFIVPLP 420

Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
           A ALN   +  VANYA S  NPHVRILKSVAI+D YAPMAAQFSSRGPNLL+PEIMKPDI
Sbjct: 421 ATALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLLPEIMKPDI 480

Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
           AAPGVEILA+ +P    S+  GD R+V+FTIMSGTSM+CPHVAGLAAYVKSFHPNWSP+A
Sbjct: 481 AAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPSA 540

Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
           IKSA+MTT              FL+GSGQINP QAIEPGLVYE  E+DYL  LCGNGFDS
Sbjct: 541 IKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKFLCGNGFDS 600

Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
           ++VR ISGN+S CSR S KF P+DLNYPAMV +V PMKPFVV F+RTV NVG ANSTYKS
Sbjct: 601 KSVRGISGNKSDCSRFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRTVTNVGTANSTYKS 660

Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
           EFSPFS VYVLKS EKL VSVEP +L F DLNEKKSFVVTV GG+IP+ T  S AL+W D
Sbjct: 661 EFSPFSIVYVLKSSEKLNVSVEPPELTFKDLNEKKSFVVTVAGGSIPARTGFSLALIWSD 720

BLAST of Tan0010374 vs. ExPASy TrEMBL
Match: A0A6J1JXM0 (subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490709 PE=3 SV=1)

HSP 1 Score: 1062.8 bits (2747), Expect = 6.5e-307
Identity = 545/733 (74.35%), Postives = 614/733 (83.77%), Query Frame = 0

Query: 1   MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
           MA  NFP  FS I ALL AIF+SANGSERK HIVYMG+I+NRA+A+STHHLNLL+SVIGT
Sbjct: 1   MAPNNFPILFSLIAALLAAIFTSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGT 60

Query: 61  SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
           S   E S+IRSYGRSFNGF AKLT AE EQLA M+GVVSVFESKELKTQTTRSWD++GF 
Sbjct: 61  SSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLATMDGVVSVFESKELKTQTTRSWDFLGF- 120

Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
           + PKRNLAGE DVIIGSID+GIWPESESFNDEGIGPPP +WRG CAGGENFTCN+KVIGA
Sbjct: 121 QQPKRNLAGELDVIIGSIDSGIWPESESFNDEGIGPPPQKWRGVCAGGENFTCNHKVIGA 180

Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
           R+YS  SARD+ GHG+HTA+TA GKSV T GFYGIAGGVARGAVPSSR+AVY  C+PSC+
Sbjct: 181 RYYSTSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSPSCT 240

Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
           +V+ILAAFDDAIADGVD+ITIS+GG      + D IAIG++HAM RGILTVQSAGNDGPV
Sbjct: 241 DVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVRGILTVQSAGNDGPV 300

Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
            GSV SVAPWLF+VAATTTDR+I+D+ VLGNGK V+G S+N F+PNKNV LIYG+NASK 
Sbjct: 301 VGSVESVAPWLFSVAATTTDRSIIDETVLGNGKIVSGYSINPFTPNKNVTLIYGTNASKS 360

Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
           CS  + E+CS  CLDP+LVKGKIVQC +F G  +A  AGA GAIVLNDKADNVSF+ PLP
Sbjct: 361 CSFQNPEVCS--CLDPKLVKGKIVQCKSFTGIFKAVDAGAAGAIVLNDKADNVSFIVPLP 420

Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
           AIALN   +  VANYA S  NPHVRILKSVAI+D YAPMAAQFSSRGPNLL+P+IMKPDI
Sbjct: 421 AIALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLLPDIMKPDI 480

Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
           AAPGVEILA+ +P    S+  GD R+V+FTIMSGTSM+CPHVAGLAAYVKSFHPNWSP+A
Sbjct: 481 AAPGVEILASVNPQPPQSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPSA 540

Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
           IKSA+MTT              FL+GSGQINP QAIEPGLVYE  E DYL  LCGNGFDS
Sbjct: 541 IKSAIMTTAEEMTNTDGFVSGEFLHGSGQINPKQAIEPGLVYEIFEVDYLKFLCGNGFDS 600

Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
           ++VR ISGN+S CS+ S KF P+DLNYPAMV +V PMKPFV+ F+RTV NVG+ANSTYKS
Sbjct: 601 KSVRGISGNKSDCSKFSTKFSPQDLNYPAMVFEVSPMKPFVIKFQRTVTNVGIANSTYKS 660

Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
           EFS FS VYVLKS EK  VSVEP +L F DLNEKKSF+VTV GG+IP+ T  SSALVW D
Sbjct: 661 EFSAFSVVYVLKSSEKPNVSVEPPELTFRDLNEKKSFIVTVTGGSIPARTGFSSALVWSD 720

BLAST of Tan0010374 vs. ExPASy TrEMBL
Match: A0A6J1H1W0 (subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459610 PE=3 SV=1)

HSP 1 Score: 1057.4 bits (2733), Expect = 2.7e-305
Identity = 532/733 (72.58%), Postives = 617/733 (84.17%), Query Frame = 0

Query: 1   MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
           MA +NFP  FSFI ALL AIF+SANGSERK HIVYMG+++NRA+A+STHHLNLL+SVIGT
Sbjct: 1   MAPENFPILFSFIAALLAAIFTSANGSERKAHIVYMGAVQNRAMAESTHHLNLLRSVIGT 60

Query: 61  SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
           S   E S+IRSYGRSFNGF AKLT  E EQLAAM+GVVSVFESK  KTQTTRSWDY+GF 
Sbjct: 61  SSVMEGSYIRSYGRSFNGFVAKLTGGEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFP 120

Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
             P RNLAGE DVIIGSIDTGIWPE ESFNDEGIGPPP RWRG C GG NFTCNNKVIGA
Sbjct: 121 AKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCTGGGNFTCNNKVIGA 180

Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
           RFY++ SARDNVGHGSHTA+TAAGKS +T GFYG+AGGVARGAVPSSR+A+YK CNP C 
Sbjct: 181 RFYTSMSARDNVGHGSHTASTAAGKSGKTTGFYGLAGGVARGAVPSSRLAIYKACNPDCL 240

Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
           E +ILAAFDDAIADGVD+ITISI G G F F+ DS+AIGS+H+MA+GILTVQSAGN GP 
Sbjct: 241 EENILAAFDDAIADGVDLITISIVGAGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPE 300

Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
           +G+VGSV PW+FTVAAT TDRTIVDKVVLG+G TV G SVN+F+PN+NVPLIY +NAS+ 
Sbjct: 301 DGTVGSVVPWVFTVAATNTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNVPLIYATNASRN 360

Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
           CSS ++EIC +GCLDP LVKGKIVQC AF+GAS AF AGA GAIVLND A NVSFV P P
Sbjct: 361 CSSKNAEICRDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFP 420

Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
           AIAL + D+ +VANYA SA NP+V I +SVA +DP+APM A FSSRGPN+ M EI+KPDI
Sbjct: 421 AIALKMADYKSVANYAISATNPNVTIFRSVATKDPHAPMIADFSSRGPNMWMLEILKPDI 480

Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
           AAPGVEILA+FSPIA+PS ++GDKRSV+F+I+SGTSMSCPHVAG+AAYVKSFHPNWSPAA
Sbjct: 481 AAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAA 540

Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
           IKSA+MTT              FLYGSG ++P++AIEPGLVYE  E+D+L++LC  G+DS
Sbjct: 541 IKSAIMTTAKQIVRTDGILIREFLYGSGLVDPDRAIEPGLVYEIFEKDHLNMLCAKGYDS 600

Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
           +T++  +GN+SVC +   K+  +DLNYPAMVA VLPMKPFVV F+RTV NVGVANSTY+S
Sbjct: 601 KTMKAFAGNDSVCPKTWTKYLARDLNYPAMVAHVLPMKPFVVKFQRTVTNVGVANSTYRS 660

Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
           +   FS V VLKS EKL VSV+PQ LWFG+LNEKKSFVVTV+GG IP++ V+SS L+W D
Sbjct: 661 KILSFSGVNVLKSLEKLSVSVKPQKLWFGNLNEKKSFVVTVVGGTIPTKIVLSSVLIWSD 720

BLAST of Tan0010374 vs. ExPASy TrEMBL
Match: A0A5C7IY30 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_002770 PE=3 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 4.3e-218
Identity = 408/746 (54.69%), Postives = 523/746 (70.11%), Query Frame = 0

Query: 1   MAKKNFPFF--FSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVI 60
           M  K F  F   SF+  + +   ++   ++R LHIVYMGS++    + ++ HL +LQ VI
Sbjct: 1   MTMKGFLLFQYCSFLLCMNILCGATDADTDRNLHIVYMGSLQEGQYSPASQHLTMLQEVI 60

Query: 61  GTSFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVG 120
           G+S + E+  +RSY RSFNGFAAKLTD E ++L++M+GVVS+F S  L  QTTRSWD++G
Sbjct: 61  GSS-SLENVLVRSYTRSFNGFAAKLTDNERQKLSSMDGVVSIFPSGTLHLQTTRSWDFMG 120

Query: 121 FGENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVI 180
           F E+  R    E+++I+G ID+GIWPES+SF+DEG GPPP +W+GAC GG+NFTCNNK+I
Sbjct: 121 FNESVSRKRKTESNIIVGVIDSGIWPESDSFSDEGFGPPPSKWKGACRGGKNFTCNNKII 180

Query: 181 GARFYSAE----SARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKV 240
           GAR+Y++     SARD  GHGSHTA+TAAG  V+   FYG+A G ARG VPS+RIA Y+V
Sbjct: 181 GARYYNSASSEISARDEQGHGSHTASTAAGNQVKDASFYGLAKGTARGGVPSARIAAYRV 240

Query: 241 CNP--SCSEVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQ 300
           C P   C++ DILAAFDDAIADGVD+ITISIGG  A  F  D IAIG+FHAMA+GILTVQ
Sbjct: 241 CYPDTGCADADILAAFDDAIADGVDLITISIGGSNAIEFNEDVIAIGAFHAMAKGILTVQ 300

Query: 301 SAGNDGPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFS-PNKNVPL 360
           +AGNDGP   SV SVAPWLF+VAA++TDR IVD ++LGNGKTV G S+NSFS   + +PL
Sbjct: 301 AAGNDGPKSSSVSSVAPWLFSVAASSTDRMIVDNIILGNGKTVQGISINSFSLDGQKLPL 360

Query: 361 IYGSNASKI---CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLND 420
           ++G     +   CS+  +E C  GCLD   VKGKI+ C    G   A +AGA G+I+L D
Sbjct: 361 VHGKEVLSLTDDCSTTSAENCFPGCLDSRKVKGKIIICDREMGYVGALRAGAAGSILLTD 420

Query: 421 KADN----VSFVFPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFS 480
            +      V+F+ PLPA A++ +++  + +Y  S KNP   ILKS AI++  AP+  +FS
Sbjct: 421 LSKEDAFLVAFIVPLPASAVSSKEYNAILSYKNSTKNPLAEILKSEAIKNTTAPVVTKFS 480

Query: 481 SRGPNLLMPEIMKPDIAAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAG 540
           SRGPN L+PEI+KPDI+APG++ILAA+SPIA  S  L DKRSV + I+SGTSMSCPHV G
Sbjct: 481 SRGPNSLVPEILKPDISAPGLQILAAYSPIAPISEYLDDKRSVKYHIISGTSMSCPHVTG 540

Query: 541 LAAYVKSFHPNWSPAAIKSAVMTT-------------FLYGSGQINPNQAIEPGLVYETS 600
           +AAYVK+FHP+WSP+AIKSA+MTT             F YGSG INP +AI PGLVYET 
Sbjct: 541 VAAYVKTFHPDWSPSAIKSAIMTTAWTMDPSKNGDGEFAYGSGHINPLEAINPGLVYETL 600

Query: 601 EEDYLDLLCGNGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFE 660
           + DY+ +LC  G   + +R ISG+ S C + S K  PKDLNYP+M AQV   KPF +TF 
Sbjct: 601 KPDYIKMLCSKGLSDKALRRISGDNSTCPKGSEKESPKDLNYPSMSAQVKQDKPFKITFN 660

Query: 661 RTVMNVGVANSTYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGA 718
           RTV NVG+ NS YK+  S          P+ + + VEP  L F  LNEKKSF +TV G A
Sbjct: 661 RTVTNVGLPNSVYKANVS---------QPQNVSIKVEPAVLSFKSLNEKKSFTITVSGSA 720

BLAST of Tan0010374 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 734.6 bits (1895), Expect = 7.8e-212
Identity = 387/699 (55.36%), Postives = 489/699 (69.96%), Query Frame = 0

Query: 36  MGSIKNRAVAKSTHHLNLLQSVIGTSFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAME 95
           MG++     +  +HHL++LQ ++GT   A    +RSY RSFNGFAA L+ AE+++L  M+
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGT-IAASHLLVRSYKRSFNGFAANLSQAESQKLQNMK 60

Query: 96  GVVSVFESKELKTQTTRSWDYVGFGENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIG 155
            VVSVF SK  +  TTRSWD+VGFGE  +R    E+DVI+G ID+GIWPESESF+DEG G
Sbjct: 61  EVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFG 120

Query: 156 PPPPRWRGACAGGENFTCNNKVIGARFYS--AESARDNVGHGSHTAATAAGKSVRTGGFY 215
           PPP +W+G+C GG  F CNNK+IGARFY+  A+SARD  GHG+HTA+TAAG +V+   FY
Sbjct: 121 PPPKKWKGSCKGGLKFACNNKLIGARFYNKFADSARDEEGHGTHTASTAAGNAVQAASFY 180

Query: 216 GIAGGVARGAVPSSRIAVYKVCNPSCSEVDILAAFDDAIADGVDVITISIGGLGAFGFKS 275
           G+A G ARG VPS+RIA YKVC   C++VDILAAFDDAIADGVDVI+ISI         +
Sbjct: 181 GLAQGTARGGVPSARIAAYKVCFNRCNDVDILAAFDDAIADGVDVISISISADYVSNLLN 240

Query: 276 DSIAIGSFHAMARGILTVQSAGNDGPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGK 335
            S+AIGSFHAM RGI+T  SAGN+GP +GSV +V+PW+ TVAA+ TDR  +D+VVLGNGK
Sbjct: 241 ASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGK 300

Query: 336 TVTGDSVNSFSPN-KNVPLIYGSNASKICSSFDSEICSEGCLDPELVKGKIVQCMAFNGA 395
            +TG SVN+F+ N    P++YG N S+ CS   +  CS GC+D ELVKGKIV C  F G 
Sbjct: 301 ALTGISVNTFNLNGTKFPIVYGQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGY 360

Query: 396 SEAFKAGADGAIVLNDKADNVSFVFPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAI 455
            EA+ AGA G IV N    + +FV P PA +L  ED+ ++ +Y  SA+ P   IL++  I
Sbjct: 361 REAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEI 420

Query: 456 RDPYAPMAAQFSSRGPNLLMPEIMKPDIAAPGVEILAAFSPIASPSNDLG--DKRSVDFT 515
            D  AP    FSSRGP+ ++  ++KPD++APG+EILAAFSP+ASPS+ L   DKRSV ++
Sbjct: 421 VDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYS 480

Query: 516 IMSGTSMSCPHVAGLAAYVKSFHPNWSPAAIKSAVMTT-------------FLYGSGQIN 575
           +MSGTSM+CPHVAG+AAYVKSFHP+WSP+AIKSA+MTT             F YGSGQIN
Sbjct: 481 VMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQIN 540

Query: 576 PNQAIEPGLVYETSEEDYLDLLCGNGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMV 635
           P +A +PGLVYE   EDYL +LC  GFDS T+   SG    CS  +     KDLNYP M 
Sbjct: 541 PTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTE---VKDLNYPTMT 600

Query: 636 AQVLPMKPFVVTFERTVMNVGVANSTYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDL 695
             V  + PF VTF+RTV NVG  NSTYK+   P         PE L++S+EP+ L FG L
Sbjct: 601 TFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPL-------QPE-LQISIEPEILRFGFL 660

Query: 696 NEKKSFVVTVIGGAIPSETVISSALVWKDGIHKVRSPIV 717
            EKKSFVVT+ G  +   + +SS++VW DG H VRSPIV
Sbjct: 661 EEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIV 687

BLAST of Tan0010374 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 681.4 bits (1757), Expect = 7.9e-196
Identity = 376/738 (50.95%), Postives = 492/738 (66.67%), Query Frame = 0

Query: 11  SFITALLVAIFSSA--NGSERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESS 70
           S I ALLV  F+SA  +  +++++IVYMG++ +R      +HH ++LQ V G S + +  
Sbjct: 11  SCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGES-SIQDR 70

Query: 71  FIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGF--GENPKR 130
            +R+Y RSFNGFAA+LT++E E LA+M+ VVSVF SK L  QTT SW+++G   G+  KR
Sbjct: 71  LVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKR 130

Query: 131 NLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYS- 190
           N   E+D IIG ID+GI+PES+SF+ +G GPPP +W+G C GG NFTCNNK+IGAR+Y+ 
Sbjct: 131 NPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYYTP 190

Query: 191 -----AESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPS- 250
                 ESARDN GHGSHTA+ AAG +V+   FYG+  G  RG VP++RIAVYKVC+P  
Sbjct: 191 KLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGV 250

Query: 251 --CSEVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGN 310
             C+   ILAAFDDAIAD VD+IT+S+G      F+ D++AIG+FHAMA+GILTV  AGN
Sbjct: 251 IRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGN 310

Query: 311 DGPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPN-KNVPLIYGS 370
           +GP   ++ S+APWLFTVAA+  +R  + KVVLGNGKT+ G SVNSF  N K  PL+YG 
Sbjct: 311 NGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVYGK 370

Query: 371 NASKICSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSF 430
           +AS  C +  +  CS GCLD + VKGKIV C       EA   GA  +IV N   D  S 
Sbjct: 371 SASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNPYEDAAS- 430

Query: 431 VFPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEI 490
           VF  P   L+ +D+  V +Y  S KNP   +LKS  I +  AP+ A +SSRGPN L+ +I
Sbjct: 431 VFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDI 490

Query: 491 MKPDIAAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPN 550
           +KPDI APG EILAA+SP   PS    D R V +T++SGTSMSCPHVAG+AAY+K+FHP 
Sbjct: 491 LKPDITAPGSEILAAYSPYVPPSE--SDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPL 550

Query: 551 WSPAAIKSAVMTT----------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLL 610
           WSP+ I+SA+MTT                F YG+G ++P  AI PGLVYE ++ D++  L
Sbjct: 551 WSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFL 610

Query: 611 CGNGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGV 670
           CG  +  + +R+ISG+ S C++   K   ++LNYP+M AQV   KPF VTF RTV NVG 
Sbjct: 611 CGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGR 670

Query: 671 ANSTYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVIS 718
            N+TYK++        V+ S  KLKV V P  L    L EKKSF VTV G    +E ++S
Sbjct: 671 PNATYKAK--------VVGS--KLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVS 730

BLAST of Tan0010374 vs. TAIR 10
Match: AT3G46840.1 (Subtilase family protein )

HSP 1 Score: 679.5 bits (1752), Expect = 3.0e-195
Identity = 379/738 (51.36%), Postives = 497/738 (67.34%), Query Frame = 0

Query: 11  SFITALLVAIFSSA--NGSERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESS 70
           S I ALLV  F+SA  +  +++ +IVYMG++  R      +HH ++LQ V G S + E  
Sbjct: 12  SCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGES-SIEDR 71

Query: 71  FIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFGEN--PKR 130
            +R+Y RSFNGFAA+LT +E E LA+M+ VVSVF +K+LK QTT SW+++G  E+   KR
Sbjct: 72  LVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRTKR 131

Query: 131 NLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYS- 190
           N   E+D IIG ID+GI+PES+SF+ +G GPPP +W+G C GG+NFT NNK+IGAR+Y+ 
Sbjct: 132 NTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYYTP 191

Query: 191 -----AESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNP-- 250
                 ESARD +GHGSHTA+TAAG +V+   FYG+  G ARG VP++RIAVYKVC+P  
Sbjct: 192 KLEGFPESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDPGV 251

Query: 251 -SCSEVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGN 310
             C+   ILAAFDDAIAD VD+ITISIGG  +  F+ D IAIG+FHAMA+GIL V SAGN
Sbjct: 252 DGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSAGN 311

Query: 311 DGPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPN-KNVPLIYGS 370
            GP   +V S+APW+FTVAA+ T+R  V KVVLGNGKTV G SVNSF  N K  PL+YG 
Sbjct: 312 SGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVYGK 371

Query: 371 NASKICSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSF 430
           +AS  C +  +  CS GCLD + VKGKIV C +     EA   GA  +IV + + D V+ 
Sbjct: 372 SASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVRSHRTD-VAS 431

Query: 431 VFPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEI 490
           +F  P   L  +D+  V +Y  S KNP   +LKS  I +  AP+ A + SRGPN ++P+I
Sbjct: 432 IFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIPDI 491

Query: 491 MKPDIAAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPN 550
           +KPDI APG EI+AA+SP A PS  + D R V +++ +GTSMSCPHVAG+AAY+KSFHP 
Sbjct: 492 LKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFHPR 551

Query: 551 WSPAAIKSAVMTT----------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLL 610
           WSP+ I+SA+MTT                F YG+G ++P  AI PGLVYE ++ D++  L
Sbjct: 552 WSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIAFL 611

Query: 611 CGNGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGV 670
           CG  + ++ +R+ISG+ S C++   K  P++LNYP+M AQV   KPF V F RTV NVG 
Sbjct: 612 CGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNVGR 671

Query: 671 ANSTYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVIS 718
            N+TYK++        V+ S  KLKV V P  L    L EKKSF VT  G    +E ++S
Sbjct: 672 PNATYKAK--------VVGS--KLKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAENLVS 731

BLAST of Tan0010374 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 672.2 bits (1733), Expect = 4.8e-193
Identity = 370/731 (50.62%), Postives = 497/731 (67.99%), Query Frame = 0

Query: 10  FSFITALLVAIFSSANGSERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESSF 69
           +S++  LL++  S+    + +++IVYMGS+ +RA    ++ H+++LQ V G S + E   
Sbjct: 11  YSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGES-SIEGRL 70

Query: 70  IRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGF--GENPKRN 129
           +RSY RSFNGFAA+LT++E   +A +EGVVSVF +K L+  TT SWD++G   G+N KRN
Sbjct: 71  VRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRN 130

Query: 130 LAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYSAE 189
           LA E+D IIG IDTGIWPES+SF+D+G GPPP +W+G C+GG+NFTCNNK+IGAR Y++E
Sbjct: 131 LAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSE 190

Query: 190 SARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPS-CSEVDIL 249
             RD  GHG+HTA+TAAG +V+   F+GI  G  RG VP+SRIA YKVC  S CS   +L
Sbjct: 191 GTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALL 250

Query: 250 AAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPVEGSVG 309
           ++FDDAIADGVD+ITISIG      F+ D IAIG+FHAMA+GILTV SAGN GP   +V 
Sbjct: 251 SSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVS 310

Query: 310 SVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFS-PNKNVPLIYG-SNASKICSS 369
            VAPW+FTVAA+TT+R  + KVVLGNGKT+ G SVN+F    K  PL+YG S AS  C +
Sbjct: 311 HVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDA 370

Query: 370 FDSEICSEGCLDPELVKGKIVQCMAFNGASEAFK-AGADGAIVLNDKA--DNVSFVFPLP 429
             + +C+  CL+   VKGKI+ C    G    +K A + GAI + DK+   +V+F   LP
Sbjct: 371 KTAALCAPACLNKSRVKGKILVC----GGPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLP 430

Query: 430 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 489
           A  L  +DF ++ +Y  S  +P   +LK+  I +  +P+ A FSSRGPN +  +I+KPDI
Sbjct: 431 ASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDI 490

Query: 490 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 549
            APGVEILAAFSP   PS D  D R V +++ SGTSM+CPHVAG+AAYVK+F+P WSP+ 
Sbjct: 491 TAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSM 550

Query: 550 IKSAVMTT----------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDSETVR 609
           I+SA+MTT          F YG+G ++P  A+ PGLVYE  + D++  LCG  + S+T++
Sbjct: 551 IQSAIMTTAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLK 610

Query: 610 IISGNESVCSRNSRKFPPKDLNYPAMVAQVLPM-KPFVVTFERTVMNVGVANSTYKSEFS 669
           IISG+   CS+ + K  P++LNYP+M A++      F VTF RT+ NVG  NSTYKS+  
Sbjct: 611 IISGDTVKCSKKN-KILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSK-- 670

Query: 670 PFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKDGIH 721
                 V     KL + V P  L+F  +NEK+SF VTV G  + SE   S+ L+W DG H
Sbjct: 671 -----VVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTH 726

BLAST of Tan0010374 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 670.6 bits (1729), Expect = 1.4e-192
Identity = 370/731 (50.62%), Postives = 487/731 (66.62%), Query Frame = 0

Query: 11  SFITALLVAIFSSANG--SERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESS 70
           S ++ LLV   SS +    +++++IVYMGS+ +RA    ++ H+N+LQ V G S + E  
Sbjct: 9   SLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGES-SIEGR 68

Query: 71  FIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGF--GENPKR 130
            +RSY RSFNGFAA+LT++E E++A M GVVSVF +K+L+ QTT SWD++G   G   KR
Sbjct: 69  LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKR 128

Query: 131 NLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYSA 190
           N   E+D IIG ID+GI PES+SF+D+G GPPP +W+G C+GG+NFTCNNK+IGAR Y++
Sbjct: 129 NPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS 188

Query: 191 ESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPS-CSEVDI 250
           E  RD  GHG+HTA+TAAG +V    F+GI  G  RG VP+SR+A YKVC P+ CS   +
Sbjct: 189 EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEAL 248

Query: 251 LAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPVEGSV 310
           L+AFDDAIADGVD+ITISIG   A  F++D IAIG+FHAMA+G+LTV SAGN GP   SV
Sbjct: 249 LSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISV 308

Query: 311 GSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFS-PNKNVPLIYG-SNASKICS 370
             VAPW+ TVAA+TT+R  V KVVLGNGKT+ G SVN++    K+ PL+YG S AS  C 
Sbjct: 309 SGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACD 368

Query: 371 SFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLPAI 430
           +  + +C   C+D   VKGKI+ C    G       GA G I    K D V+F+ PLPA 
Sbjct: 369 AESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAVGLIYRTPKPD-VAFIHPLPAA 428

Query: 431 ALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDIAA 490
            L  EDF ++ +Y  S  +P   +LK+ AI +  +P+ A FSSRGPN +  +I+KPDI A
Sbjct: 429 GLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITA 488

Query: 491 PGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAAIK 550
           PGVEILAA+SP   PS D  D R V ++++SGTSMSCPHVAG+AAYVK+F+P WSP+ I+
Sbjct: 489 PGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQ 548

Query: 551 SAVMTT---------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDSE 610
           SA+MTT               F YGSG ++P  A  PGLVYE  + D++  LCG  + S+
Sbjct: 549 SAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQ 608

Query: 611 TVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPM-KPFVVTFERTVMNVGVANSTYKS 670
            +++ISG    CS  ++K  P++LNYP+M A++      F VTF RT+ NVG  NSTY S
Sbjct: 609 VLKVISGETVTCS-EAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTS 668

Query: 671 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 718
           +        V     KL V + P  L F  +NEK+SF VTV G  + SE   S+ L+W D
Sbjct: 669 K-------VVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSD 727

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FIF81.2e-21253.74Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... [more]
Q9STF71.1e-19450.95Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S... [more]
F4JA914.2e-19451.36Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 S... [more]
Q9FIG22.0e-19150.62Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... [more]
Q8L7D22.5e-19150.27Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... [more]
Match NameE-valueIdentityDescription
KAG6605933.16.6e-30974.76Subtilisin-like protease 4.3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022995031.19.3e-30873.94subtilisin-like protease SBT4.3 [Cucurbita maxima][more]
XP_023533940.13.5e-30774.22subtilisin-like protease SBT4.3 [Cucurbita pepo subsp. pepo][more]
XP_022958362.14.6e-30774.35subtilisin-like protease SBT4.3 [Cucurbita moschata][more]
XP_023533978.14.6e-30773.53subtilisin-like protease SBT4.3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1K0V84.5e-30873.94subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490708 PE=3... [more]
A0A6J1H1M32.2e-30774.35subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459607 PE... [more]
A0A6J1JXM06.5e-30774.35subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490709 PE=3... [more]
A0A6J1H1W02.7e-30572.58subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459610 PE... [more]
A0A5C7IY304.3e-21854.69Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_002770 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G59190.17.8e-21255.36subtilase family protein [more]
AT3G46850.17.9e-19650.95Subtilase family protein [more]
AT3G46840.13.0e-19551.36Subtilase family protein [more]
AT5G59090.24.8e-19350.62subtilase 4.12 [more]
AT5G59120.11.4e-19250.62subtilase 4.13 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 130..149
score: 26.7
coord: 513..529
score: 57.2
coord: 190..203
score: 42.55
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 132..575
e-value: 2.8E-162
score: 542.7
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 109..566
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 132..557
e-value: 1.2E-40
score: 139.5
NoneNo IPR availableGENE3D3.50.30.30coord: 320..453
e-value: 2.8E-162
score: 542.7
NoneNo IPR availableGENE3D2.60.40.2310coord: 579..720
e-value: 4.0E-33
score: 115.9
NoneNo IPR availablePANTHERPTHR10795:SF685SUBTILISIN-LIKE PROTEASE SBT4.3coord: 31..718
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 113..574
score: 24.950335
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 327..446
e-value: 2.21675E-20
score: 85.5431
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 610..716
e-value: 1.7E-21
score: 76.3
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 18..108
e-value: 7.2E-22
score: 79.6
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 32..108
e-value: 5.6E-11
score: 43.0
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 31..718
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 514..524
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 106..548
e-value: 5.08172E-121
score: 362.301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0010374.1Tan0010374.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity