Homology
BLAST of Tan0010374 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 741.1 bits (1912), Expect = 1.2e-212
Identity = 395/735 (53.74%), Postives = 506/735 (68.84%), Query Frame = 0
Query: 1 MAKKNFPFFFSFITALLVAIFSSANGSE-RKLHIVYMGSIKNRAVAKSTHHLNLLQSVIG 60
MAK + P + + + S+ + + ++IVYMG++ + +HHL++LQ ++G
Sbjct: 1 MAKLSTPLYLICLAFIFTRDVSANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVG 60
Query: 61 TSFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGF 120
T A +RSY RSFNGFAA L+ AE+++L M+ VVSVF SK + TTRSWD+VGF
Sbjct: 61 T-IAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGF 120
Query: 121 GENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIG 180
GE +R E+DVI+G ID+GIWPESESF+DEG GPPP +W+G+C GG F CNNK+IG
Sbjct: 121 GEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIG 180
Query: 181 ARFYS--AESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNP 240
ARFY+ A+SARD GHG+HTA+TAAG +V+ FYG+A G ARG VPS+RIA YKVC
Sbjct: 181 ARFYNKFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFN 240
Query: 241 SCSEVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGND 300
C++VDILAAFDDAIADGVDVI+ISI + S+AIGSFHAM RGI+T SAGN+
Sbjct: 241 RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNN 300
Query: 301 GPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPN-KNVPLIYGSN 360
GP +GSV +V+PW+ TVAA+ TDR +D+VVLGNGK +TG SVN+F+ N P++YG N
Sbjct: 301 GPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQN 360
Query: 361 ASKICSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFV 420
S+ CS + CS GC+D ELVKGKIV C F G EA+ AGA G IV N + +FV
Sbjct: 361 VSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAFV 420
Query: 421 FPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIM 480
P PA +L ED+ ++ +Y SA+ P IL++ I D AP FSSRGP+ ++ ++
Sbjct: 421 VPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLL 480
Query: 481 KPDIAAPGVEILAAFSPIASPSNDLG--DKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHP 540
KPD++APG+EILAAFSP+ASPS+ L DKRSV +++MSGTSM+CPHVAG+AAYVKSFHP
Sbjct: 481 KPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHP 540
Query: 541 NWSPAAIKSAVMTT-------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCG 600
+WSP+AIKSA+MTT F YGSGQINP +A +PGLVYE EDYL +LC
Sbjct: 541 DWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCA 600
Query: 601 NGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVAN 660
GFDS T+ SG CS + KDLNYP M V + PF VTF+RTV NVG N
Sbjct: 601 EGFDSTTLTTTSGQNVTCSERTE---VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPN 660
Query: 661 STYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSA 717
STYK+ P PE L++S+EP+ L FG L EKKSFVVT+ G + + +SS+
Sbjct: 661 STYKASVVPL-------QPE-LQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSS 720
BLAST of Tan0010374 vs. ExPASy Swiss-Prot
Match:
Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)
HSP 1 Score: 681.4 bits (1757), Expect = 1.1e-194
Identity = 376/738 (50.95%), Postives = 492/738 (66.67%), Query Frame = 0
Query: 11 SFITALLVAIFSSA--NGSERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESS 70
S I ALLV F+SA + +++++IVYMG++ +R +HH ++LQ V G S + +
Sbjct: 11 SCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGES-SIQDR 70
Query: 71 FIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGF--GENPKR 130
+R+Y RSFNGFAA+LT++E E LA+M+ VVSVF SK L QTT SW+++G G+ KR
Sbjct: 71 LVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKR 130
Query: 131 NLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYS- 190
N E+D IIG ID+GI+PES+SF+ +G GPPP +W+G C GG NFTCNNK+IGAR+Y+
Sbjct: 131 NPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYYTP 190
Query: 191 -----AESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPS- 250
ESARDN GHGSHTA+ AAG +V+ FYG+ G RG VP++RIAVYKVC+P
Sbjct: 191 KLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGV 250
Query: 251 --CSEVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGN 310
C+ ILAAFDDAIAD VD+IT+S+G F+ D++AIG+FHAMA+GILTV AGN
Sbjct: 251 IRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGN 310
Query: 311 DGPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPN-KNVPLIYGS 370
+GP ++ S+APWLFTVAA+ +R + KVVLGNGKT+ G SVNSF N K PL+YG
Sbjct: 311 NGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVYGK 370
Query: 371 NASKICSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSF 430
+AS C + + CS GCLD + VKGKIV C EA GA +IV N D S
Sbjct: 371 SASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNPYEDAAS- 430
Query: 431 VFPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEI 490
VF P L+ +D+ V +Y S KNP +LKS I + AP+ A +SSRGPN L+ +I
Sbjct: 431 VFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDI 490
Query: 491 MKPDIAAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPN 550
+KPDI APG EILAA+SP PS D R V +T++SGTSMSCPHVAG+AAY+K+FHP
Sbjct: 491 LKPDITAPGSEILAAYSPYVPPSE--SDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPL 550
Query: 551 WSPAAIKSAVMTT----------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLL 610
WSP+ I+SA+MTT F YG+G ++P AI PGLVYE ++ D++ L
Sbjct: 551 WSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFL 610
Query: 611 CGNGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGV 670
CG + + +R+ISG+ S C++ K ++LNYP+M AQV KPF VTF RTV NVG
Sbjct: 611 CGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGR 670
Query: 671 ANSTYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVIS 718
N+TYK++ V+ S KLKV V P L L EKKSF VTV G +E ++S
Sbjct: 671 PNATYKAK--------VVGS--KLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVS 730
BLAST of Tan0010374 vs. ExPASy Swiss-Prot
Match:
F4JA91 (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)
HSP 1 Score: 679.5 bits (1752), Expect = 4.2e-194
Identity = 379/738 (51.36%), Postives = 497/738 (67.34%), Query Frame = 0
Query: 11 SFITALLVAIFSSA--NGSERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESS 70
S I ALLV F+SA + +++ +IVYMG++ R +HH ++LQ V G S + E
Sbjct: 12 SCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGES-SIEDR 71
Query: 71 FIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFGEN--PKR 130
+R+Y RSFNGFAA+LT +E E LA+M+ VVSVF +K+LK QTT SW+++G E+ KR
Sbjct: 72 LVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRTKR 131
Query: 131 NLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYS- 190
N E+D IIG ID+GI+PES+SF+ +G GPPP +W+G C GG+NFT NNK+IGAR+Y+
Sbjct: 132 NTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYYTP 191
Query: 191 -----AESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNP-- 250
ESARD +GHGSHTA+TAAG +V+ FYG+ G ARG VP++RIAVYKVC+P
Sbjct: 192 KLEGFPESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDPGV 251
Query: 251 -SCSEVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGN 310
C+ ILAAFDDAIAD VD+ITISIGG + F+ D IAIG+FHAMA+GIL V SAGN
Sbjct: 252 DGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSAGN 311
Query: 311 DGPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPN-KNVPLIYGS 370
GP +V S+APW+FTVAA+ T+R V KVVLGNGKTV G SVNSF N K PL+YG
Sbjct: 312 SGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVYGK 371
Query: 371 NASKICSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSF 430
+AS C + + CS GCLD + VKGKIV C + EA GA +IV + + D V+
Sbjct: 372 SASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVRSHRTD-VAS 431
Query: 431 VFPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEI 490
+F P L +D+ V +Y S KNP +LKS I + AP+ A + SRGPN ++P+I
Sbjct: 432 IFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIPDI 491
Query: 491 MKPDIAAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPN 550
+KPDI APG EI+AA+SP A PS + D R V +++ +GTSMSCPHVAG+AAY+KSFHP
Sbjct: 492 LKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFHPR 551
Query: 551 WSPAAIKSAVMTT----------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLL 610
WSP+ I+SA+MTT F YG+G ++P AI PGLVYE ++ D++ L
Sbjct: 552 WSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIAFL 611
Query: 611 CGNGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGV 670
CG + ++ +R+ISG+ S C++ K P++LNYP+M AQV KPF V F RTV NVG
Sbjct: 612 CGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNVGR 671
Query: 671 ANSTYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVIS 718
N+TYK++ V+ S KLKV V P L L EKKSF VT G +E ++S
Sbjct: 672 PNATYKAK--------VVGS--KLKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAENLVS 731
BLAST of Tan0010374 vs. ExPASy Swiss-Prot
Match:
Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)
HSP 1 Score: 670.6 bits (1729), Expect = 2.0e-191
Identity = 370/731 (50.62%), Postives = 487/731 (66.62%), Query Frame = 0
Query: 11 SFITALLVAIFSSANG--SERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESS 70
S ++ LLV SS + +++++IVYMGS+ +RA ++ H+N+LQ V G S + E
Sbjct: 9 SLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGES-SIEGR 68
Query: 71 FIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGF--GENPKR 130
+RSY RSFNGFAA+LT++E E++A M GVVSVF +K+L+ QTT SWD++G G KR
Sbjct: 69 LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKR 128
Query: 131 NLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYSA 190
N E+D IIG ID+GI PES+SF+D+G GPPP +W+G C+GG+NFTCNNK+IGAR Y++
Sbjct: 129 NPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS 188
Query: 191 ESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPS-CSEVDI 250
E RD GHG+HTA+TAAG +V F+GI G RG VP+SR+A YKVC P+ CS +
Sbjct: 189 EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEAL 248
Query: 251 LAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPVEGSV 310
L+AFDDAIADGVD+ITISIG A F++D IAIG+FHAMA+G+LTV SAGN GP SV
Sbjct: 249 LSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISV 308
Query: 311 GSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFS-PNKNVPLIYG-SNASKICS 370
VAPW+ TVAA+TT+R V KVVLGNGKT+ G SVN++ K+ PL+YG S AS C
Sbjct: 309 SGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACD 368
Query: 371 SFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLPAI 430
+ + +C C+D VKGKI+ C G GA G I K D V+F+ PLPA
Sbjct: 369 AESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAVGLIYRTPKPD-VAFIHPLPAA 428
Query: 431 ALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDIAA 490
L EDF ++ +Y S +P +LK+ AI + +P+ A FSSRGPN + +I+KPDI A
Sbjct: 429 GLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITA 488
Query: 491 PGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAAIK 550
PGVEILAA+SP PS D D R V ++++SGTSMSCPHVAG+AAYVK+F+P WSP+ I+
Sbjct: 489 PGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQ 548
Query: 551 SAVMTT---------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDSE 610
SA+MTT F YGSG ++P A PGLVYE + D++ LCG + S+
Sbjct: 549 SAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQ 608
Query: 611 TVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPM-KPFVVTFERTVMNVGVANSTYKS 670
+++ISG CS ++K P++LNYP+M A++ F VTF RT+ NVG NSTY S
Sbjct: 609 VLKVISGETVTCS-EAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTS 668
Query: 671 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 718
+ V KL V + P L F +NEK+SF VTV G + SE S+ L+W D
Sbjct: 669 K-------VVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSD 727
BLAST of Tan0010374 vs. ExPASy Swiss-Prot
Match:
Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)
HSP 1 Score: 670.2 bits (1728), Expect = 2.5e-191
Identity = 370/736 (50.27%), Postives = 497/736 (67.53%), Query Frame = 0
Query: 10 FSFITALLVAIFSSANGSERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESSF 69
+S++ LL++ S+ + +++IVYMGS+ +RA ++ H+++LQ V G S + E
Sbjct: 11 YSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGES-SIEGRL 70
Query: 70 IRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGF--GENPKRN 129
+RSY RSFNGFAA+LT++E +A +EGVVSVF +K L+ TT SWD++G G+N KRN
Sbjct: 71 VRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRN 130
Query: 130 LAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYSAE 189
LA E+D IIG IDTGIWPES+SF+D+G GPPP +W+G C+GG+NFTCNNK+IGAR Y++E
Sbjct: 131 LAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSE 190
Query: 190 SARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPS-CSEVDIL 249
RD GHG+HTA+TAAG +V+ F+GI G RG VP+SRIA YKVC S CS +L
Sbjct: 191 GTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALL 250
Query: 250 AAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPVEGSVG 309
++FDDAIADGVD+ITISIG F+ D IAIG+FHAMA+GILTV SAGN GP +V
Sbjct: 251 SSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVS 310
Query: 310 SVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFS-PNKNVPLIYG-SNASKICSS 369
VAPW+FTVAA+TT+R + KVVLGNGKT+ G SVN+F K PL+YG S AS C +
Sbjct: 311 HVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDA 370
Query: 370 FDSEICSEGCLDPELVKGKIVQCMAFNGASEAFK-AGADGAIVLNDKA--DNVSFVFPLP 429
+ +C+ CL+ VKGKI+ C G +K A + GAI + DK+ +V+F LP
Sbjct: 371 KTAALCAPACLNKSRVKGKILVC----GGPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLP 430
Query: 430 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 489
A L +DF ++ +Y S +P +LK+ I + +P+ A FSSRGPN + +I+KPDI
Sbjct: 431 ASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDI 490
Query: 490 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 549
APGVEILAAFSP PS D D R V +++ SGTSM+CPHVAG+AAYVK+F+P WSP+
Sbjct: 491 TAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSM 550
Query: 550 IKSAVMTT---------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFD 609
I+SA+MTT F YG+G ++P A+ PGLVYE + D++ LCG +
Sbjct: 551 IQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYT 610
Query: 610 SETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPM-KPFVVTFERTVMNVGVANSTY 669
S+T++IISG+ CS+ + K P++LNYP+M A++ F VTF RT+ NVG NSTY
Sbjct: 611 SKTLKIISGDTVKCSKKN-KILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTY 670
Query: 670 KSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVW 721
KS+ V KL + V P L+F +NEK+SF VTV G + SE S+ L+W
Sbjct: 671 KSK-------VVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIW 730
BLAST of Tan0010374 vs. NCBI nr
Match:
KAG6605933.1 (Subtilisin-like protease 4.3, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1070.5 bits (2767), Expect = 6.6e-309
Identity = 548/733 (74.76%), Postives = 620/733 (84.58%), Query Frame = 0
Query: 1 MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
MA NFP FFSFI ALL AIF+SANGSERK HIVYMG+I+NRA+A+STHHLNLL+SVIGT
Sbjct: 1 MAANNFPIFFSFIAALLAAIFTSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGT 60
Query: 61 SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
S E S+IRSYGRSFNGF A+LT E EQLAAM+GVVSVFESKELKT+TTRSWD++GF
Sbjct: 61 SSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSWDFLGF- 120
Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
+ PKRNLAGE DVIIGSID+GIWPESESFNDEGIGPPP +WRG CAGGENFTCN+KVIGA
Sbjct: 121 QQPKRNLAGELDVIIGSIDSGIWPESESFNDEGIGPPPQKWRGVCAGGENFTCNHKVIGA 180
Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
R+YS+ SARD+ GHG+HTA+TA GKSV T GFYGIAGGVARGAVPSSR+AVY C+PSC+
Sbjct: 181 RYYSSSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSPSCT 240
Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
+V+ILAAFDDAIADGVD+ITIS+GG + D IAIG++HAM +GILTVQSAGNDGPV
Sbjct: 241 DVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQSAGNDGPV 300
Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
GSV SVAPWLF+VAATTTDR+IVD++VLGNGKTV+G S+N F+PNK+VPLIYG+NASK
Sbjct: 301 VGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDVPLIYGTNASKS 360
Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
CS + E+CS CLDP+LVKGKIVQC +F G +A AGA GAIVLNDKADNVSF+ PLP
Sbjct: 361 CSFQNPEVCS--CLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADNVSFIVPLP 420
Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
AIALN + V NYA S NPHVRILKSVAI+D YAPMAAQFSSRGPNLL+PEIMKPDI
Sbjct: 421 AIALNFTAYEQVTNYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLLPEIMKPDI 480
Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
AAPGVEILA+ +P S+ GD R+V+FTIMSGTSM+CPHVAGLAAYVKSFHPNWSP+A
Sbjct: 481 AAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPSA 540
Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
IKSA+MTT FL+GSGQINP QAIEPGLVYE E+DYL LCGNGFDS
Sbjct: 541 IKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKFLCGNGFDS 600
Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
++VR ISGN+S CS+ S KF P+DLNYPAMV +V PMKPFVV F+R V NVG ANSTYKS
Sbjct: 601 KSVRGISGNKSDCSKFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRIVTNVGTANSTYKS 660
Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
EFSPFS VYVLKS EKL VSVEP +L F DLNEKKSFVVTV GG+IP+ T SSAL+W D
Sbjct: 661 EFSPFSIVYVLKSSEKLNVSVEPTELTFKDLNEKKSFVVTVAGGSIPARTGFSSALIWSD 720
BLAST of Tan0010374 vs. NCBI nr
Match:
XP_022995031.1 (subtilisin-like protease SBT4.3 [Cucurbita maxima])
HSP 1 Score: 1066.6 bits (2757), Expect = 9.3e-308
Identity = 542/733 (73.94%), Postives = 618/733 (84.31%), Query Frame = 0
Query: 1 MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
MA NFP FSFI ALL AI SSANGSERK HIVYMG+I+NRA+A+STHHLNLL+SVIGT
Sbjct: 1 MAPDNFPILFSFIAALLAAILSSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGT 60
Query: 61 SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
S E S+IRSYGRSFNGF AKLT AE EQLAAM+GVVSVFESK KTQTTRSWDY+GF
Sbjct: 61 SSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFP 120
Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
P RNLAGE DVIIGSIDTGIWPE ESFNDEGIGPPP RWRG CAGG NFTCNNKVIGA
Sbjct: 121 AKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGA 180
Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
RFY++ SARDNVGHGSHTA+TAAGK +T GFYG+AGG ARGAVPSSR+AVYKVCNP C
Sbjct: 181 RFYTSMSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNPDCL 240
Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
E +ILAAFDDAIADGVD+ITISIGG+G F F+ DS+AIGS+H+MA+GILTVQSAGN GPV
Sbjct: 241 EENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPV 300
Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
+G+VGSV PW+FTVAATTTDRTIVDKVVLG+G TV G SVN+F+PN+NVPLIY +NAS+
Sbjct: 301 DGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNVPLIYATNASRN 360
Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
CSS ++EICS+GCLDP LVKGKIVQC AF+GAS AF AGA GAIVLND A NVSFV P P
Sbjct: 361 CSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFP 420
Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
AIAL + D+ +VANYA SA NP+V I +SVA +DPYAP A FSSRGPN+ M EI+KPDI
Sbjct: 421 AIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILKPDI 480
Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
AAPGVEILA+FSPIA+PS ++GDKRSV+F+I+SGTSMSCPHVAG+AAYVKSFHPNWSPAA
Sbjct: 481 AAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAA 540
Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
IKSA+MTT FLYGSG I+PN+AIEPGLVYE E+D+L++LC G+DS
Sbjct: 541 IKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDS 600
Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
+T+ +GN+SVCS+ KF +DLNYPAMVA VLP KPFVV F+RTV NVGVANSTY+S
Sbjct: 601 KTMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNVGVANSTYRS 660
Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
+ FS V VLKS EKL VSV+PQ+LWF LNEKKSFVVTV+GGAI +E V SSAL+W D
Sbjct: 661 KILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSD 720
BLAST of Tan0010374 vs. NCBI nr
Match:
XP_023533940.1 (subtilisin-like protease SBT4.3 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1064.7 bits (2752), Expect = 3.5e-307
Identity = 544/733 (74.22%), Postives = 621/733 (84.72%), Query Frame = 0
Query: 1 MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
MA NFP FSFI ALL AIF+SANGSERK HIVYMG+I+NRA+A+STHHLNLLQSVIGT
Sbjct: 1 MAANNFPISFSFIAALLAAIFTSANGSERKDHIVYMGAIQNRAMAESTHHLNLLQSVIGT 60
Query: 61 SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
S E S+IRSYGRSFNGF A+LT E EQLAAM+GVVSVFESKELKT+TTRSWD++GF
Sbjct: 61 SSVTEDSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSWDFLGF- 120
Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
+ PKRNLAGE DVIIGSID+GIWPESESFNDEG+GPPP +WRG CAGGENFTCN+KVIGA
Sbjct: 121 QQPKRNLAGELDVIIGSIDSGIWPESESFNDEGMGPPPQKWRGECAGGENFTCNHKVIGA 180
Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
R+YS+ SARD+ GHG+HTA+TA GKSV T GFYGIAGGVARGAVPSSR+AVY C+PSC+
Sbjct: 181 RYYSSSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSPSCT 240
Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
+V+ILAAFDDAIADGVD+ITIS+GG + D IAIG++HAM +GILTVQSAGNDGPV
Sbjct: 241 DVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQSAGNDGPV 300
Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
GSV SVAPWLF+VAATTTDR+IVD++VLGNGKTV+G S+N F+PNK+VPLIYG+NASK
Sbjct: 301 VGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDVPLIYGTNASKS 360
Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
CS + E+CS CLDP+LVKGKIVQC +F G +A AGA GAIVLNDKADNVSF+ PLP
Sbjct: 361 CSFQNPEVCS--CLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADNVSFIVPLP 420
Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
AIALN+ + VANYA S NPHVRIL+SVA++D YAPMAAQFSSRGPNLL+PEIMKPDI
Sbjct: 421 AIALNLTAYEQVANYAISEPNPHVRILQSVAVKDGYAPMAAQFSSRGPNLLLPEIMKPDI 480
Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
AAPGVEILA+ +P S+ GD R+V+FTIMSGTSM+CPHVAGLAAYVKSFHPNWSP+A
Sbjct: 481 AAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPSA 540
Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
IKSA+MTT FL+GSGQI+P QAIEPGLVYE E+DYL LCGNGFDS
Sbjct: 541 IKSAIMTTAEEMTNTDGFLSGEFLHGSGQIDPKQAIEPGLVYEIFEDDYLKFLCGNGFDS 600
Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
++VR ISGN+S CS+ S KF P+DLNYPAMV +V PMKPF V F+RTV NVG+ANSTYKS
Sbjct: 601 KSVRGISGNKSDCSKFSTKFSPQDLNYPAMVFEVPPMKPFAVKFQRTVTNVGIANSTYKS 660
Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
EFSP S VYVLKS EKL VSVEP +L F DLNEKKSFVVTV GG+IP+ T SSAL+W D
Sbjct: 661 EFSPLSVVYVLKSSEKLNVSVEPPELTFRDLNEKKSFVVTVAGGSIPARTGFSSALIWSD 720
BLAST of Tan0010374 vs. NCBI nr
Match:
XP_022958362.1 (subtilisin-like protease SBT4.3 [Cucurbita moschata])
HSP 1 Score: 1064.3 bits (2751), Expect = 4.6e-307
Identity = 545/733 (74.35%), Postives = 619/733 (84.45%), Query Frame = 0
Query: 1 MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
MA NFP F+FI ALL AIF+SANGSERK HIVYMG+I+NRA+A+STHHLNLL+SVIGT
Sbjct: 1 MAAINFPILFTFIAALLAAIFTSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGT 60
Query: 61 SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
S E S+IRSYGRSFNGF A+LT E EQLAAM+GVVSVFESKELKT+TTRSWD++GF
Sbjct: 61 SSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSWDFLGF- 120
Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
+ PKRNL+GE DVIIGSID+GIWPESESFND+GIGPPP +WRG CAGGENFTCN+KVIGA
Sbjct: 121 QQPKRNLSGELDVIIGSIDSGIWPESESFNDDGIGPPPQKWRGVCAGGENFTCNHKVIGA 180
Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
R+YS+ SARD+ GHG+HTA+TA GKSV T GFYGIAGGVARGAVPSSR+AVY C+PSC+
Sbjct: 181 RYYSSSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSPSCT 240
Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
+V+ILAAFDDAIADGVD+ITIS+GG + D IAIG++HAM +GILTVQSAGNDGPV
Sbjct: 241 DVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQSAGNDGPV 300
Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
GSV SVAPWLF+VAATTTDR+IVD++VLGNGKTV+G S+N F+PNK+VPLIYG+NASK
Sbjct: 301 VGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDVPLIYGTNASKS 360
Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
CS + E+CS CLDP+LVKGKIVQC +F G +A AGA GAIVLNDKADNVSF+ PLP
Sbjct: 361 CSFQNPEVCS--CLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADNVSFIVPLP 420
Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
A ALN + VANYA S NPHVRILKSVAI+D YAPMAAQFSSRGPNLL+PEIMKPDI
Sbjct: 421 ATALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLLPEIMKPDI 480
Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
AAPGVEILA+ +P S+ GD R+V+FTIMSGTSM+CPHVAGLAAYVKSFHPNWSP+A
Sbjct: 481 AAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPSA 540
Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
IKSA+MTT FL+GSGQINP QAIEPGLVYE E+DYL LCGNGFDS
Sbjct: 541 IKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKFLCGNGFDS 600
Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
++VR ISGN+S CSR S KF P+DLNYPAMV +V PMKPFVV F+RTV NVG ANSTYKS
Sbjct: 601 KSVRGISGNKSDCSRFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRTVTNVGTANSTYKS 660
Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
EFSPFS VYVLKS EKL VSVEP +L F DLNEKKSFVVTV GG+IP+ T S AL+W D
Sbjct: 661 EFSPFSIVYVLKSSEKLNVSVEPPELTFKDLNEKKSFVVTVAGGSIPARTGFSLALIWSD 720
BLAST of Tan0010374 vs. NCBI nr
Match:
XP_023533978.1 (subtilisin-like protease SBT4.3 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1064.3 bits (2751), Expect = 4.6e-307
Identity = 539/733 (73.53%), Postives = 619/733 (84.45%), Query Frame = 0
Query: 1 MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
MA NFP FSFI ALL AIF+SANGSERK HIVYMG+I+NRA+A+STHHLNLL+SVIG+
Sbjct: 1 MAPDNFPILFSFIAALLAAIFTSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGS 60
Query: 61 SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
S E S+IRSYGRSFNGF AKLT E EQLAAM+GVVSVFESK KTQTTRSWDY+GF
Sbjct: 61 SSVMEGSYIRSYGRSFNGFVAKLTGGEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFP 120
Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
P RNLAGE DVIIGSIDTGIWPE ESFNDEGIGPPP RWRG CAGG NFTCNNKVIGA
Sbjct: 121 AKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGA 180
Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
RFY++ SARDNVGHGSHTA+TAAGKS +T GFYG+AGGVARGAVPSSR+AVYK CNP C
Sbjct: 181 RFYTSMSARDNVGHGSHTASTAAGKSGKTTGFYGLAGGVARGAVPSSRLAVYKACNPDCL 240
Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
+ +ILAAFDDAIADGVD+ITISI G G F F+ DS+AIGS+H+MA+GILTVQSAGN GP
Sbjct: 241 DENILAAFDDAIADGVDLITISIVGAGGFNFEQDSMAIGSYHSMAKGILTVQSAGNYGPE 300
Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
+G+VGSV PW+FTVAAT TDRTIVDKVVLG+G TV G SVN+F+PN+NVPLIY +NAS+
Sbjct: 301 DGTVGSVVPWVFTVAATNTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNVPLIYATNASRN 360
Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
CSS ++EIC +GCLDP LVKGKIVQC AF+GAS AF AGA G IVLNDKA NVSFV P P
Sbjct: 361 CSSKNAEICRDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGGIVLNDKAANVSFVLPFP 420
Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
AIAL + D+ +VANYA SA NP+V I +SVA +DPYAP A FSSRGPN+ M EI+KPDI
Sbjct: 421 AIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILKPDI 480
Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
AAPGVEILA+FSPIA+PS ++GDKRSV+F+I+SGTSMSCPHVAG+AAYVKSFHPNWSPAA
Sbjct: 481 AAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAA 540
Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
IKSA+MTT FLYGSG I+PN+AIEPGLVYE E+D+L++LC G+DS
Sbjct: 541 IKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDS 600
Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
+T++ GN+SVCS+ S K+ +DLNYPAMVA VLPMKPFVV F+RT+ NVGVANSTY+S
Sbjct: 601 KTMKAFVGNDSVCSKTSTKYLARDLNYPAMVAHVLPMKPFVVKFQRTLTNVGVANSTYRS 660
Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
+ S FS V VLKS EKL VSV+PQ+LWF +LNEKKSFVVTV GGAIP+E V+SSAL+W D
Sbjct: 661 KISLFSGVNVLKSLEKLNVSVKPQELWFRNLNEKKSFVVTVAGGAIPTEIVLSSALIWSD 720
BLAST of Tan0010374 vs. ExPASy TrEMBL
Match:
A0A6J1K0V8 (subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490708 PE=3 SV=1)
HSP 1 Score: 1066.6 bits (2757), Expect = 4.5e-308
Identity = 542/733 (73.94%), Postives = 618/733 (84.31%), Query Frame = 0
Query: 1 MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
MA NFP FSFI ALL AI SSANGSERK HIVYMG+I+NRA+A+STHHLNLL+SVIGT
Sbjct: 1 MAPDNFPILFSFIAALLAAILSSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGT 60
Query: 61 SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
S E S+IRSYGRSFNGF AKLT AE EQLAAM+GVVSVFESK KTQTTRSWDY+GF
Sbjct: 61 SSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFP 120
Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
P RNLAGE DVIIGSIDTGIWPE ESFNDEGIGPPP RWRG CAGG NFTCNNKVIGA
Sbjct: 121 AKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGA 180
Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
RFY++ SARDNVGHGSHTA+TAAGK +T GFYG+AGG ARGAVPSSR+AVYKVCNP C
Sbjct: 181 RFYTSMSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNPDCL 240
Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
E +ILAAFDDAIADGVD+ITISIGG+G F F+ DS+AIGS+H+MA+GILTVQSAGN GPV
Sbjct: 241 EENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPV 300
Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
+G+VGSV PW+FTVAATTTDRTIVDKVVLG+G TV G SVN+F+PN+NVPLIY +NAS+
Sbjct: 301 DGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNVPLIYATNASRN 360
Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
CSS ++EICS+GCLDP LVKGKIVQC AF+GAS AF AGA GAIVLND A NVSFV P P
Sbjct: 361 CSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFP 420
Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
AIAL + D+ +VANYA SA NP+V I +SVA +DPYAP A FSSRGPN+ M EI+KPDI
Sbjct: 421 AIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILKPDI 480
Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
AAPGVEILA+FSPIA+PS ++GDKRSV+F+I+SGTSMSCPHVAG+AAYVKSFHPNWSPAA
Sbjct: 481 AAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAA 540
Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
IKSA+MTT FLYGSG I+PN+AIEPGLVYE E+D+L++LC G+DS
Sbjct: 541 IKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDS 600
Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
+T+ +GN+SVCS+ KF +DLNYPAMVA VLP KPFVV F+RTV NVGVANSTY+S
Sbjct: 601 KTMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNVGVANSTYRS 660
Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
+ FS V VLKS EKL VSV+PQ+LWF LNEKKSFVVTV+GGAI +E V SSAL+W D
Sbjct: 661 KILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSD 720
BLAST of Tan0010374 vs. ExPASy TrEMBL
Match:
A0A6J1H1M3 (subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459607 PE=3 SV=1)
HSP 1 Score: 1064.3 bits (2751), Expect = 2.2e-307
Identity = 545/733 (74.35%), Postives = 619/733 (84.45%), Query Frame = 0
Query: 1 MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
MA NFP F+FI ALL AIF+SANGSERK HIVYMG+I+NRA+A+STHHLNLL+SVIGT
Sbjct: 1 MAAINFPILFTFIAALLAAIFTSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGT 60
Query: 61 SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
S E S+IRSYGRSFNGF A+LT E EQLAAM+GVVSVFESKELKT+TTRSWD++GF
Sbjct: 61 SSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSWDFLGF- 120
Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
+ PKRNL+GE DVIIGSID+GIWPESESFND+GIGPPP +WRG CAGGENFTCN+KVIGA
Sbjct: 121 QQPKRNLSGELDVIIGSIDSGIWPESESFNDDGIGPPPQKWRGVCAGGENFTCNHKVIGA 180
Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
R+YS+ SARD+ GHG+HTA+TA GKSV T GFYGIAGGVARGAVPSSR+AVY C+PSC+
Sbjct: 181 RYYSSSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSPSCT 240
Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
+V+ILAAFDDAIADGVD+ITIS+GG + D IAIG++HAM +GILTVQSAGNDGPV
Sbjct: 241 DVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQSAGNDGPV 300
Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
GSV SVAPWLF+VAATTTDR+IVD++VLGNGKTV+G S+N F+PNK+VPLIYG+NASK
Sbjct: 301 VGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDVPLIYGTNASKS 360
Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
CS + E+CS CLDP+LVKGKIVQC +F G +A AGA GAIVLNDKADNVSF+ PLP
Sbjct: 361 CSFQNPEVCS--CLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADNVSFIVPLP 420
Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
A ALN + VANYA S NPHVRILKSVAI+D YAPMAAQFSSRGPNLL+PEIMKPDI
Sbjct: 421 ATALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLLPEIMKPDI 480
Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
AAPGVEILA+ +P S+ GD R+V+FTIMSGTSM+CPHVAGLAAYVKSFHPNWSP+A
Sbjct: 481 AAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPSA 540
Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
IKSA+MTT FL+GSGQINP QAIEPGLVYE E+DYL LCGNGFDS
Sbjct: 541 IKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKFLCGNGFDS 600
Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
++VR ISGN+S CSR S KF P+DLNYPAMV +V PMKPFVV F+RTV NVG ANSTYKS
Sbjct: 601 KSVRGISGNKSDCSRFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRTVTNVGTANSTYKS 660
Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
EFSPFS VYVLKS EKL VSVEP +L F DLNEKKSFVVTV GG+IP+ T S AL+W D
Sbjct: 661 EFSPFSIVYVLKSSEKLNVSVEPPELTFKDLNEKKSFVVTVAGGSIPARTGFSLALIWSD 720
BLAST of Tan0010374 vs. ExPASy TrEMBL
Match:
A0A6J1JXM0 (subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490709 PE=3 SV=1)
HSP 1 Score: 1062.8 bits (2747), Expect = 6.5e-307
Identity = 545/733 (74.35%), Postives = 614/733 (83.77%), Query Frame = 0
Query: 1 MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
MA NFP FS I ALL AIF+SANGSERK HIVYMG+I+NRA+A+STHHLNLL+SVIGT
Sbjct: 1 MAPNNFPILFSLIAALLAAIFTSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGT 60
Query: 61 SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
S E S+IRSYGRSFNGF AKLT AE EQLA M+GVVSVFESKELKTQTTRSWD++GF
Sbjct: 61 SSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLATMDGVVSVFESKELKTQTTRSWDFLGF- 120
Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
+ PKRNLAGE DVIIGSID+GIWPESESFNDEGIGPPP +WRG CAGGENFTCN+KVIGA
Sbjct: 121 QQPKRNLAGELDVIIGSIDSGIWPESESFNDEGIGPPPQKWRGVCAGGENFTCNHKVIGA 180
Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
R+YS SARD+ GHG+HTA+TA GKSV T GFYGIAGGVARGAVPSSR+AVY C+PSC+
Sbjct: 181 RYYSTSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYSACSPSCT 240
Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
+V+ILAAFDDAIADGVD+ITIS+GG + D IAIG++HAM RGILTVQSAGNDGPV
Sbjct: 241 DVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVRGILTVQSAGNDGPV 300
Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
GSV SVAPWLF+VAATTTDR+I+D+ VLGNGK V+G S+N F+PNKNV LIYG+NASK
Sbjct: 301 VGSVESVAPWLFSVAATTTDRSIIDETVLGNGKIVSGYSINPFTPNKNVTLIYGTNASKS 360
Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
CS + E+CS CLDP+LVKGKIVQC +F G +A AGA GAIVLNDKADNVSF+ PLP
Sbjct: 361 CSFQNPEVCS--CLDPKLVKGKIVQCKSFTGIFKAVDAGAAGAIVLNDKADNVSFIVPLP 420
Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
AIALN + VANYA S NPHVRILKSVAI+D YAPMAAQFSSRGPNLL+P+IMKPDI
Sbjct: 421 AIALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLLPDIMKPDI 480
Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
AAPGVEILA+ +P S+ GD R+V+FTIMSGTSM+CPHVAGLAAYVKSFHPNWSP+A
Sbjct: 481 AAPGVEILASVNPQPPQSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSFHPNWSPSA 540
Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
IKSA+MTT FL+GSGQINP QAIEPGLVYE E DYL LCGNGFDS
Sbjct: 541 IKSAIMTTAEEMTNTDGFVSGEFLHGSGQINPKQAIEPGLVYEIFEVDYLKFLCGNGFDS 600
Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
++VR ISGN+S CS+ S KF P+DLNYPAMV +V PMKPFV+ F+RTV NVG+ANSTYKS
Sbjct: 601 KSVRGISGNKSDCSKFSTKFSPQDLNYPAMVFEVSPMKPFVIKFQRTVTNVGIANSTYKS 660
Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
EFS FS VYVLKS EK VSVEP +L F DLNEKKSF+VTV GG+IP+ T SSALVW D
Sbjct: 661 EFSAFSVVYVLKSSEKPNVSVEPPELTFRDLNEKKSFIVTVTGGSIPARTGFSSALVWSD 720
BLAST of Tan0010374 vs. ExPASy TrEMBL
Match:
A0A6J1H1W0 (subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459610 PE=3 SV=1)
HSP 1 Score: 1057.4 bits (2733), Expect = 2.7e-305
Identity = 532/733 (72.58%), Postives = 617/733 (84.17%), Query Frame = 0
Query: 1 MAKKNFPFFFSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVIGT 60
MA +NFP FSFI ALL AIF+SANGSERK HIVYMG+++NRA+A+STHHLNLL+SVIGT
Sbjct: 1 MAPENFPILFSFIAALLAAIFTSANGSERKAHIVYMGAVQNRAMAESTHHLNLLRSVIGT 60
Query: 61 SFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFG 120
S E S+IRSYGRSFNGF AKLT E EQLAAM+GVVSVFESK KTQTTRSWDY+GF
Sbjct: 61 SSVMEGSYIRSYGRSFNGFVAKLTGGEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFP 120
Query: 121 ENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGA 180
P RNLAGE DVIIGSIDTGIWPE ESFNDEGIGPPP RWRG C GG NFTCNNKVIGA
Sbjct: 121 AKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCTGGGNFTCNNKVIGA 180
Query: 181 RFYSAESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPSCS 240
RFY++ SARDNVGHGSHTA+TAAGKS +T GFYG+AGGVARGAVPSSR+A+YK CNP C
Sbjct: 181 RFYTSMSARDNVGHGSHTASTAAGKSGKTTGFYGLAGGVARGAVPSSRLAIYKACNPDCL 240
Query: 241 EVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPV 300
E +ILAAFDDAIADGVD+ITISI G G F F+ DS+AIGS+H+MA+GILTVQSAGN GP
Sbjct: 241 EENILAAFDDAIADGVDLITISIVGAGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPE 300
Query: 301 EGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPNKNVPLIYGSNASKI 360
+G+VGSV PW+FTVAAT TDRTIVDKVVLG+G TV G SVN+F+PN+NVPLIY +NAS+
Sbjct: 301 DGTVGSVVPWVFTVAATNTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNVPLIYATNASRN 360
Query: 361 CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLP 420
CSS ++EIC +GCLDP LVKGKIVQC AF+GAS AF AGA GAIVLND A NVSFV P P
Sbjct: 361 CSSKNAEICRDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFP 420
Query: 421 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 480
AIAL + D+ +VANYA SA NP+V I +SVA +DP+APM A FSSRGPN+ M EI+KPDI
Sbjct: 421 AIALKMADYKSVANYAISATNPNVTIFRSVATKDPHAPMIADFSSRGPNMWMLEILKPDI 480
Query: 481 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 540
AAPGVEILA+FSPIA+PS ++GDKRSV+F+I+SGTSMSCPHVAG+AAYVKSFHPNWSPAA
Sbjct: 481 AAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAA 540
Query: 541 IKSAVMTT--------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDS 600
IKSA+MTT FLYGSG ++P++AIEPGLVYE E+D+L++LC G+DS
Sbjct: 541 IKSAIMTTAKQIVRTDGILIREFLYGSGLVDPDRAIEPGLVYEIFEKDHLNMLCAKGYDS 600
Query: 601 ETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGVANSTYKS 660
+T++ +GN+SVC + K+ +DLNYPAMVA VLPMKPFVV F+RTV NVGVANSTY+S
Sbjct: 601 KTMKAFAGNDSVCPKTWTKYLARDLNYPAMVAHVLPMKPFVVKFQRTVTNVGVANSTYRS 660
Query: 661 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 720
+ FS V VLKS EKL VSV+PQ LWFG+LNEKKSFVVTV+GG IP++ V+SS L+W D
Sbjct: 661 KILSFSGVNVLKSLEKLSVSVKPQKLWFGNLNEKKSFVVTVVGGTIPTKIVLSSVLIWSD 720
BLAST of Tan0010374 vs. ExPASy TrEMBL
Match:
A0A5C7IY30 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_002770 PE=3 SV=1)
HSP 1 Score: 767.7 bits (1981), Expect = 4.3e-218
Identity = 408/746 (54.69%), Postives = 523/746 (70.11%), Query Frame = 0
Query: 1 MAKKNFPFF--FSFITALLVAIFSSANGSERKLHIVYMGSIKNRAVAKSTHHLNLLQSVI 60
M K F F SF+ + + ++ ++R LHIVYMGS++ + ++ HL +LQ VI
Sbjct: 1 MTMKGFLLFQYCSFLLCMNILCGATDADTDRNLHIVYMGSLQEGQYSPASQHLTMLQEVI 60
Query: 61 GTSFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVG 120
G+S + E+ +RSY RSFNGFAAKLTD E ++L++M+GVVS+F S L QTTRSWD++G
Sbjct: 61 GSS-SLENVLVRSYTRSFNGFAAKLTDNERQKLSSMDGVVSIFPSGTLHLQTTRSWDFMG 120
Query: 121 FGENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVI 180
F E+ R E+++I+G ID+GIWPES+SF+DEG GPPP +W+GAC GG+NFTCNNK+I
Sbjct: 121 FNESVSRKRKTESNIIVGVIDSGIWPESDSFSDEGFGPPPSKWKGACRGGKNFTCNNKII 180
Query: 181 GARFYSAE----SARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKV 240
GAR+Y++ SARD GHGSHTA+TAAG V+ FYG+A G ARG VPS+RIA Y+V
Sbjct: 181 GARYYNSASSEISARDEQGHGSHTASTAAGNQVKDASFYGLAKGTARGGVPSARIAAYRV 240
Query: 241 CNP--SCSEVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQ 300
C P C++ DILAAFDDAIADGVD+ITISIGG A F D IAIG+FHAMA+GILTVQ
Sbjct: 241 CYPDTGCADADILAAFDDAIADGVDLITISIGGSNAIEFNEDVIAIGAFHAMAKGILTVQ 300
Query: 301 SAGNDGPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFS-PNKNVPL 360
+AGNDGP SV SVAPWLF+VAA++TDR IVD ++LGNGKTV G S+NSFS + +PL
Sbjct: 301 AAGNDGPKSSSVSSVAPWLFSVAASSTDRMIVDNIILGNGKTVQGISINSFSLDGQKLPL 360
Query: 361 IYGSNASKI---CSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLND 420
++G + CS+ +E C GCLD VKGKI+ C G A +AGA G+I+L D
Sbjct: 361 VHGKEVLSLTDDCSTTSAENCFPGCLDSRKVKGKIIICDREMGYVGALRAGAAGSILLTD 420
Query: 421 KADN----VSFVFPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFS 480
+ V+F+ PLPA A++ +++ + +Y S KNP ILKS AI++ AP+ +FS
Sbjct: 421 LSKEDAFLVAFIVPLPASAVSSKEYNAILSYKNSTKNPLAEILKSEAIKNTTAPVVTKFS 480
Query: 481 SRGPNLLMPEIMKPDIAAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAG 540
SRGPN L+PEI+KPDI+APG++ILAA+SPIA S L DKRSV + I+SGTSMSCPHV G
Sbjct: 481 SRGPNSLVPEILKPDISAPGLQILAAYSPIAPISEYLDDKRSVKYHIISGTSMSCPHVTG 540
Query: 541 LAAYVKSFHPNWSPAAIKSAVMTT-------------FLYGSGQINPNQAIEPGLVYETS 600
+AAYVK+FHP+WSP+AIKSA+MTT F YGSG INP +AI PGLVYET
Sbjct: 541 VAAYVKTFHPDWSPSAIKSAIMTTAWTMDPSKNGDGEFAYGSGHINPLEAINPGLVYETL 600
Query: 601 EEDYLDLLCGNGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFE 660
+ DY+ +LC G + +R ISG+ S C + S K PKDLNYP+M AQV KPF +TF
Sbjct: 601 KPDYIKMLCSKGLSDKALRRISGDNSTCPKGSEKESPKDLNYPSMSAQVKQDKPFKITFN 660
Query: 661 RTVMNVGVANSTYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGA 718
RTV NVG+ NS YK+ S P+ + + VEP L F LNEKKSF +TV G A
Sbjct: 661 RTVTNVGLPNSVYKANVS---------QPQNVSIKVEPAVLSFKSLNEKKSFTITVSGSA 720
BLAST of Tan0010374 vs. TAIR 10
Match:
AT5G59190.1 (subtilase family protein )
HSP 1 Score: 734.6 bits (1895), Expect = 7.8e-212
Identity = 387/699 (55.36%), Postives = 489/699 (69.96%), Query Frame = 0
Query: 36 MGSIKNRAVAKSTHHLNLLQSVIGTSFNAESSFIRSYGRSFNGFAAKLTDAETEQLAAME 95
MG++ + +HHL++LQ ++GT A +RSY RSFNGFAA L+ AE+++L M+
Sbjct: 1 MGTLPEIKYSPPSHHLSILQKLVGT-IAASHLLVRSYKRSFNGFAANLSQAESQKLQNMK 60
Query: 96 GVVSVFESKELKTQTTRSWDYVGFGENPKRNLAGEADVIIGSIDTGIWPESESFNDEGIG 155
VVSVF SK + TTRSWD+VGFGE +R E+DVI+G ID+GIWPESESF+DEG G
Sbjct: 61 EVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFG 120
Query: 156 PPPPRWRGACAGGENFTCNNKVIGARFYS--AESARDNVGHGSHTAATAAGKSVRTGGFY 215
PPP +W+G+C GG F CNNK+IGARFY+ A+SARD GHG+HTA+TAAG +V+ FY
Sbjct: 121 PPPKKWKGSCKGGLKFACNNKLIGARFYNKFADSARDEEGHGTHTASTAAGNAVQAASFY 180
Query: 216 GIAGGVARGAVPSSRIAVYKVCNPSCSEVDILAAFDDAIADGVDVITISIGGLGAFGFKS 275
G+A G ARG VPS+RIA YKVC C++VDILAAFDDAIADGVDVI+ISI +
Sbjct: 181 GLAQGTARGGVPSARIAAYKVCFNRCNDVDILAAFDDAIADGVDVISISISADYVSNLLN 240
Query: 276 DSIAIGSFHAMARGILTVQSAGNDGPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGK 335
S+AIGSFHAM RGI+T SAGN+GP +GSV +V+PW+ TVAA+ TDR +D+VVLGNGK
Sbjct: 241 ASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGK 300
Query: 336 TVTGDSVNSFSPN-KNVPLIYGSNASKICSSFDSEICSEGCLDPELVKGKIVQCMAFNGA 395
+TG SVN+F+ N P++YG N S+ CS + CS GC+D ELVKGKIV C F G
Sbjct: 301 ALTGISVNTFNLNGTKFPIVYGQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGY 360
Query: 396 SEAFKAGADGAIVLNDKADNVSFVFPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAI 455
EA+ AGA G IV N + +FV P PA +L ED+ ++ +Y SA+ P IL++ I
Sbjct: 361 REAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEI 420
Query: 456 RDPYAPMAAQFSSRGPNLLMPEIMKPDIAAPGVEILAAFSPIASPSNDLG--DKRSVDFT 515
D AP FSSRGP+ ++ ++KPD++APG+EILAAFSP+ASPS+ L DKRSV ++
Sbjct: 421 VDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYS 480
Query: 516 IMSGTSMSCPHVAGLAAYVKSFHPNWSPAAIKSAVMTT-------------FLYGSGQIN 575
+MSGTSM+CPHVAG+AAYVKSFHP+WSP+AIKSA+MTT F YGSGQIN
Sbjct: 481 VMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQIN 540
Query: 576 PNQAIEPGLVYETSEEDYLDLLCGNGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMV 635
P +A +PGLVYE EDYL +LC GFDS T+ SG CS + KDLNYP M
Sbjct: 541 PTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTE---VKDLNYPTMT 600
Query: 636 AQVLPMKPFVVTFERTVMNVGVANSTYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDL 695
V + PF VTF+RTV NVG NSTYK+ P PE L++S+EP+ L FG L
Sbjct: 601 TFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPL-------QPE-LQISIEPEILRFGFL 660
Query: 696 NEKKSFVVTVIGGAIPSETVISSALVWKDGIHKVRSPIV 717
EKKSFVVT+ G + + +SS++VW DG H VRSPIV
Sbjct: 661 EEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIV 687
BLAST of Tan0010374 vs. TAIR 10
Match:
AT3G46850.1 (Subtilase family protein )
HSP 1 Score: 681.4 bits (1757), Expect = 7.9e-196
Identity = 376/738 (50.95%), Postives = 492/738 (66.67%), Query Frame = 0
Query: 11 SFITALLVAIFSSA--NGSERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESS 70
S I ALLV F+SA + +++++IVYMG++ +R +HH ++LQ V G S + +
Sbjct: 11 SCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGES-SIQDR 70
Query: 71 FIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGF--GENPKR 130
+R+Y RSFNGFAA+LT++E E LA+M+ VVSVF SK L QTT SW+++G G+ KR
Sbjct: 71 LVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKR 130
Query: 131 NLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYS- 190
N E+D IIG ID+GI+PES+SF+ +G GPPP +W+G C GG NFTCNNK+IGAR+Y+
Sbjct: 131 NPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYYTP 190
Query: 191 -----AESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPS- 250
ESARDN GHGSHTA+ AAG +V+ FYG+ G RG VP++RIAVYKVC+P
Sbjct: 191 KLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGV 250
Query: 251 --CSEVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGN 310
C+ ILAAFDDAIAD VD+IT+S+G F+ D++AIG+FHAMA+GILTV AGN
Sbjct: 251 IRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGN 310
Query: 311 DGPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPN-KNVPLIYGS 370
+GP ++ S+APWLFTVAA+ +R + KVVLGNGKT+ G SVNSF N K PL+YG
Sbjct: 311 NGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVYGK 370
Query: 371 NASKICSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSF 430
+AS C + + CS GCLD + VKGKIV C EA GA +IV N D S
Sbjct: 371 SASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNPYEDAAS- 430
Query: 431 VFPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEI 490
VF P L+ +D+ V +Y S KNP +LKS I + AP+ A +SSRGPN L+ +I
Sbjct: 431 VFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDI 490
Query: 491 MKPDIAAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPN 550
+KPDI APG EILAA+SP PS D R V +T++SGTSMSCPHVAG+AAY+K+FHP
Sbjct: 491 LKPDITAPGSEILAAYSPYVPPSE--SDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPL 550
Query: 551 WSPAAIKSAVMTT----------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLL 610
WSP+ I+SA+MTT F YG+G ++P AI PGLVYE ++ D++ L
Sbjct: 551 WSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFL 610
Query: 611 CGNGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGV 670
CG + + +R+ISG+ S C++ K ++LNYP+M AQV KPF VTF RTV NVG
Sbjct: 611 CGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGR 670
Query: 671 ANSTYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVIS 718
N+TYK++ V+ S KLKV V P L L EKKSF VTV G +E ++S
Sbjct: 671 PNATYKAK--------VVGS--KLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVS 730
BLAST of Tan0010374 vs. TAIR 10
Match:
AT3G46840.1 (Subtilase family protein )
HSP 1 Score: 679.5 bits (1752), Expect = 3.0e-195
Identity = 379/738 (51.36%), Postives = 497/738 (67.34%), Query Frame = 0
Query: 11 SFITALLVAIFSSA--NGSERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESS 70
S I ALLV F+SA + +++ +IVYMG++ R +HH ++LQ V G S + E
Sbjct: 12 SCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGES-SIEDR 71
Query: 71 FIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGFGEN--PKR 130
+R+Y RSFNGFAA+LT +E E LA+M+ VVSVF +K+LK QTT SW+++G E+ KR
Sbjct: 72 LVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRTKR 131
Query: 131 NLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYS- 190
N E+D IIG ID+GI+PES+SF+ +G GPPP +W+G C GG+NFT NNK+IGAR+Y+
Sbjct: 132 NTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYYTP 191
Query: 191 -----AESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNP-- 250
ESARD +GHGSHTA+TAAG +V+ FYG+ G ARG VP++RIAVYKVC+P
Sbjct: 192 KLEGFPESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDPGV 251
Query: 251 -SCSEVDILAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGN 310
C+ ILAAFDDAIAD VD+ITISIGG + F+ D IAIG+FHAMA+GIL V SAGN
Sbjct: 252 DGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSAGN 311
Query: 311 DGPVEGSVGSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFSPN-KNVPLIYGS 370
GP +V S+APW+FTVAA+ T+R V KVVLGNGKTV G SVNSF N K PL+YG
Sbjct: 312 SGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVYGK 371
Query: 371 NASKICSSFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSF 430
+AS C + + CS GCLD + VKGKIV C + EA GA +IV + + D V+
Sbjct: 372 SASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVRSHRTD-VAS 431
Query: 431 VFPLPAIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEI 490
+F P L +D+ V +Y S KNP +LKS I + AP+ A + SRGPN ++P+I
Sbjct: 432 IFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIPDI 491
Query: 491 MKPDIAAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPN 550
+KPDI APG EI+AA+SP A PS + D R V +++ +GTSMSCPHVAG+AAY+KSFHP
Sbjct: 492 LKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFHPR 551
Query: 551 WSPAAIKSAVMTT----------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLL 610
WSP+ I+SA+MTT F YG+G ++P AI PGLVYE ++ D++ L
Sbjct: 552 WSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIAFL 611
Query: 611 CGNGFDSETVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPMKPFVVTFERTVMNVGV 670
CG + ++ +R+ISG+ S C++ K P++LNYP+M AQV KPF V F RTV NVG
Sbjct: 612 CGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNVGR 671
Query: 671 ANSTYKSEFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVIS 718
N+TYK++ V+ S KLKV V P L L EKKSF VT G +E ++S
Sbjct: 672 PNATYKAK--------VVGS--KLKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAENLVS 731
BLAST of Tan0010374 vs. TAIR 10
Match:
AT5G59090.2 (subtilase 4.12 )
HSP 1 Score: 672.2 bits (1733), Expect = 4.8e-193
Identity = 370/731 (50.62%), Postives = 497/731 (67.99%), Query Frame = 0
Query: 10 FSFITALLVAIFSSANGSERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESSF 69
+S++ LL++ S+ + +++IVYMGS+ +RA ++ H+++LQ V G S + E
Sbjct: 11 YSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGES-SIEGRL 70
Query: 70 IRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGF--GENPKRN 129
+RSY RSFNGFAA+LT++E +A +EGVVSVF +K L+ TT SWD++G G+N KRN
Sbjct: 71 VRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRN 130
Query: 130 LAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYSAE 189
LA E+D IIG IDTGIWPES+SF+D+G GPPP +W+G C+GG+NFTCNNK+IGAR Y++E
Sbjct: 131 LAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSE 190
Query: 190 SARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPS-CSEVDIL 249
RD GHG+HTA+TAAG +V+ F+GI G RG VP+SRIA YKVC S CS +L
Sbjct: 191 GTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALL 250
Query: 250 AAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPVEGSVG 309
++FDDAIADGVD+ITISIG F+ D IAIG+FHAMA+GILTV SAGN GP +V
Sbjct: 251 SSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVS 310
Query: 310 SVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFS-PNKNVPLIYG-SNASKICSS 369
VAPW+FTVAA+TT+R + KVVLGNGKT+ G SVN+F K PL+YG S AS C +
Sbjct: 311 HVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDA 370
Query: 370 FDSEICSEGCLDPELVKGKIVQCMAFNGASEAFK-AGADGAIVLNDKA--DNVSFVFPLP 429
+ +C+ CL+ VKGKI+ C G +K A + GAI + DK+ +V+F LP
Sbjct: 371 KTAALCAPACLNKSRVKGKILVC----GGPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLP 430
Query: 430 AIALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDI 489
A L +DF ++ +Y S +P +LK+ I + +P+ A FSSRGPN + +I+KPDI
Sbjct: 431 ASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDI 490
Query: 490 AAPGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAA 549
APGVEILAAFSP PS D D R V +++ SGTSM+CPHVAG+AAYVK+F+P WSP+
Sbjct: 491 TAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSM 550
Query: 550 IKSAVMTT----------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDSETVR 609
I+SA+MTT F YG+G ++P A+ PGLVYE + D++ LCG + S+T++
Sbjct: 551 IQSAIMTTAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLK 610
Query: 610 IISGNESVCSRNSRKFPPKDLNYPAMVAQVLPM-KPFVVTFERTVMNVGVANSTYKSEFS 669
IISG+ CS+ + K P++LNYP+M A++ F VTF RT+ NVG NSTYKS+
Sbjct: 611 IISGDTVKCSKKN-KILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSK-- 670
Query: 670 PFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKDGIH 721
V KL + V P L+F +NEK+SF VTV G + SE S+ L+W DG H
Sbjct: 671 -----VVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTH 726
BLAST of Tan0010374 vs. TAIR 10
Match:
AT5G59120.1 (subtilase 4.13 )
HSP 1 Score: 670.6 bits (1729), Expect = 1.4e-192
Identity = 370/731 (50.62%), Postives = 487/731 (66.62%), Query Frame = 0
Query: 11 SFITALLVAIFSSANG--SERKLHIVYMGSIKNRA-VAKSTHHLNLLQSVIGTSFNAESS 70
S ++ LLV SS + +++++IVYMGS+ +RA ++ H+N+LQ V G S + E
Sbjct: 9 SLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGES-SIEGR 68
Query: 71 FIRSYGRSFNGFAAKLTDAETEQLAAMEGVVSVFESKELKTQTTRSWDYVGF--GENPKR 130
+RSY RSFNGFAA+LT++E E++A M GVVSVF +K+L+ QTT SWD++G G KR
Sbjct: 69 LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKR 128
Query: 131 NLAGEADVIIGSIDTGIWPESESFNDEGIGPPPPRWRGACAGGENFTCNNKVIGARFYSA 190
N E+D IIG ID+GI PES+SF+D+G GPPP +W+G C+GG+NFTCNNK+IGAR Y++
Sbjct: 129 NPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS 188
Query: 191 ESARDNVGHGSHTAATAAGKSVRTGGFYGIAGGVARGAVPSSRIAVYKVCNPS-CSEVDI 250
E RD GHG+HTA+TAAG +V F+GI G RG VP+SR+A YKVC P+ CS +
Sbjct: 189 EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEAL 248
Query: 251 LAAFDDAIADGVDVITISIGGLGAFGFKSDSIAIGSFHAMARGILTVQSAGNDGPVEGSV 310
L+AFDDAIADGVD+ITISIG A F++D IAIG+FHAMA+G+LTV SAGN GP SV
Sbjct: 249 LSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISV 308
Query: 311 GSVAPWLFTVAATTTDRTIVDKVVLGNGKTVTGDSVNSFS-PNKNVPLIYG-SNASKICS 370
VAPW+ TVAA+TT+R V KVVLGNGKT+ G SVN++ K+ PL+YG S AS C
Sbjct: 309 SGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACD 368
Query: 371 SFDSEICSEGCLDPELVKGKIVQCMAFNGASEAFKAGADGAIVLNDKADNVSFVFPLPAI 430
+ + +C C+D VKGKI+ C G GA G I K D V+F+ PLPA
Sbjct: 369 AESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAVGLIYRTPKPD-VAFIHPLPAA 428
Query: 431 ALNIEDFTNVANYATSAKNPHVRILKSVAIRDPYAPMAAQFSSRGPNLLMPEIMKPDIAA 490
L EDF ++ +Y S +P +LK+ AI + +P+ A FSSRGPN + +I+KPDI A
Sbjct: 429 GLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITA 488
Query: 491 PGVEILAAFSPIASPSNDLGDKRSVDFTIMSGTSMSCPHVAGLAAYVKSFHPNWSPAAIK 550
PGVEILAA+SP PS D D R V ++++SGTSMSCPHVAG+AAYVK+F+P WSP+ I+
Sbjct: 489 PGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQ 548
Query: 551 SAVMTT---------------FLYGSGQINPNQAIEPGLVYETSEEDYLDLLCGNGFDSE 610
SA+MTT F YGSG ++P A PGLVYE + D++ LCG + S+
Sbjct: 549 SAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQ 608
Query: 611 TVRIISGNESVCSRNSRKFPPKDLNYPAMVAQVLPM-KPFVVTFERTVMNVGVANSTYKS 670
+++ISG CS ++K P++LNYP+M A++ F VTF RT+ NVG NSTY S
Sbjct: 609 VLKVISGETVTCS-EAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTS 668
Query: 671 EFSPFSKVYVLKSPEKLKVSVEPQDLWFGDLNEKKSFVVTVIGGAIPSETVISSALVWKD 718
+ V KL V + P L F +NEK+SF VTV G + SE S+ L+W D
Sbjct: 669 K-------VVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSD 727
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9FIF8 | 1.2e-212 | 53.74 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
Q9STF7 | 1.1e-194 | 50.95 | Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S... | [more] |
F4JA91 | 4.2e-194 | 51.36 | Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 S... | [more] |
Q9FIG2 | 2.0e-191 | 50.62 | Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... | [more] |
Q8L7D2 | 2.5e-191 | 50.27 | Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... | [more] |
Match Name | E-value | Identity | Description | |
KAG6605933.1 | 6.6e-309 | 74.76 | Subtilisin-like protease 4.3, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
XP_022995031.1 | 9.3e-308 | 73.94 | subtilisin-like protease SBT4.3 [Cucurbita maxima] | [more] |
XP_023533940.1 | 3.5e-307 | 74.22 | subtilisin-like protease SBT4.3 [Cucurbita pepo subsp. pepo] | [more] |
XP_022958362.1 | 4.6e-307 | 74.35 | subtilisin-like protease SBT4.3 [Cucurbita moschata] | [more] |
XP_023533978.1 | 4.6e-307 | 73.53 | subtilisin-like protease SBT4.3 [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1K0V8 | 4.5e-308 | 73.94 | subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490708 PE=3... | [more] |
A0A6J1H1M3 | 2.2e-307 | 74.35 | subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459607 PE... | [more] |
A0A6J1JXM0 | 6.5e-307 | 74.35 | subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490709 PE=3... | [more] |
A0A6J1H1W0 | 2.7e-305 | 72.58 | subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459610 PE... | [more] |
A0A5C7IY30 | 4.3e-218 | 54.69 | Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_002770 PE=3 SV=1 | [more] |