Tan0010267 (gene) Snake gourd v1

Overview
NameTan0010267
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionRibonuclease 3-like protein 1
LocationLG04: 84309445 .. 84314094 (-)
RNA-Seq ExpressionTan0010267
SyntenyTan0010267
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AATGGAGTTACTTTACTATAAACCAGATATAGAGAGAGAAGAAGAGGAGGAAGAAGAAGAAGAAGGAATTATGAAGAAGAAGTGAATTTGTTCAGAAGAGGTCACTAGCAGAGGTCAGATTCTATTCAGAGAAACTTTGCCATTTCTTCGAATCAGAACTGTCATTTTTTTCTCTGATTTATCTTTCCTTCCTAGGATTATAATGCTTTCTATTGCTGTGAATCTTTAATTTGCTGATTTTGCATTCAATTTGATGTATAAGACAGTGCAGAGAGATCATTGGAGCCGAGCGGAAGAATCGAGGAAAGTAGATGGAGAATACACCTTCCTACACTCGAAGATTAAATGTCGATCTCAAAAAACTACCTCCAATTAATAATCCGCGCATTGGAAATGGAAATCACTCCTCGATTCCGGTGAAACTCGGTTGTTTTTCTGCAATTGTATCCGGTTTTCTTTGAAATTTCAAAAGGAATTTGATGCTGATGCTGATGCTGATTTGATTTGATTCAGGCTAAATTTGAGAAATCCAGGTATATTAGGCGTGTGCCTCAGTTTATGCCGTCGAATTACCGGCCGGAAATGAGGCCGAACGAATACGGTGGCTTGCCGATGAAACCTGTGCCTTCTGATTCCGCTCGAGCTTTGAATACGAAGACGATTCCGGAGGCTTCATCGGATTCCTCCGATAAAAAGAAAGAGAATAGCCGGGAAATAGCTTCGAGTTGCAGTTGCAGACATGGCTCGACCAAAGAAGGTACCGATTCAAACCTAGTTCCTCAACTTGATTACTATTTGCGATTCGAAATCGATGACTCCTGTTACGTTTCGTCAATTTCTTGGATTATTCTTCTCTTCGTGATTTTTCTTGTTTTCTTCTCATTGATTTGCAAATGCAATTGAATGTTCATCTCACTTGCATTCATCGAAGTTTTCTATGGTAAGTATTTGGTCTCTAACATTTCCAAAACTGAATTCTGAATCATTTCCCTGATTGTGTTTGAAGATTATCATTACTTTCCTCCCTTTCTTATTAGAGAGAGGGATGGATTTATTTTTCTTCTATTGTTATTCAGATTTCGTTTTCTGTTCTTGTTTGTCTTCAATTCTTCTCAGGCGACTTGTTTTCTAAATTACATGTATTCCAACCCACACAGTAACAATAAATTATTCAACCGCAATCTTTAATGGCTGAAAGTTATGAGAATAGTTTTCTAAACTAAGTTATTAGATTCTCAAAGTTACCAACCCTCAAGGGATGCCCTGCTCGTAAGAGCTGAAATCTCAAAATCCCACTTCAAAGATGTAAAAGAACAATTTAAACTCATTTGATAACTAGTTAATTTTCCGTTTTTGATTTTTTATAATTAAGTTTATGAACACAACTTCTACTCGTGGTTTCATTGTTTTATTATCTATTTTTTATAAATGTTTTCAAAATTTATGATAAGTTTTGGAAGCTAAAAAAATAGTTTTTGAGGTTCCTTTCTTTTATAATCTATTTTTTTACTAATGTTTTTAAATTCATGCCATGTTTTGAAAACTAAAAAAATAGTTTTTAAAAATTTGTTTTAATGATTGAAATTTGATTGGAAATTCAAATCTTTCTTCTAAGAAAGACAAAAGACTAGTAAAAAAAAATGGTTGGAAAACAAACACAAATTTTCAATAAGAGAGCTAAAAATGAAATGGTTATCCATGGGAGCTTTAATCATTTTAAATGTTTGGTGTCGTTTAGAGCTTGGTTTTGGAGCTGAGGTCGGTGGAGACTAATGGAGTGGGTCCCATTCTCCAGTTACCAATTAAAAAGAAAAAACTCACAGGAATAAAATAAGGAGAAACTAACTTAGAAATCAGGTATTCAAACATGTTTATACCCTTTTTTTTAATCGAAAGTAACTTTGGAACTCAACTAGAAAAAAGCAGGCAGGCTCTTGCACTTGGATGGCTTACTTGAGTTTTGTTAAAGCTGGACAAGGCAGGCATATTGCTAACTTTCTATCGACACTTGTTTTCATATAACTTTCTGCATTCCTTATTACAGCTCGAGTACATTGGTTTTTTTGGATTCGGATAATCTGATTCTGATCCAGAAATTTCCATTTTAGTATACTTAAAGTTTGCTCTAATGGTTTAGCGCTTTACCTTTCCATTTTAGGTACTCCAGATAAGAAATGGGCAAAGTCCCTTCTGTTTGAGATTTGCACTGCAAATCACTGGCAGCCTCCTCTGTTTGAATGCTGCGAGGAAGAAGGTCCAAGCCATGCAAGAAAGTAAGCTCAATGACTATCATGAACATGAGTAAGCAACAGTAGTTATGAAAGTATAACCACTTTATTCTAGTAACATCTCAACGCCAAACTTTCTAAAGATGTAGTCCTTATTCTGCTTTTGGTCTTGTGGGTTACTTACTAAAACTCGAGAAAAATTTCTATCTAACAACTACTTTTCGAAGCTGTTATAAATTTTCTGCCTTTCATCTTCAAAATGGGGATACCTAAGGCTCTATTTGATGACCATTTGATTTTTAGTTTTTGATTTTTGAAAATTAAGCCTATAAATACTACTTTCATCTATAGATTTTCATGTTTTGTTATATATTTTTTTCCTATGTTTTCAAAAACCAAGCTAAAATTTTGAAAACTAAAAAAATAGTTTTCAAAAACTTGTTTTTGTTTTTGGAACTTTGCGAGAAACATGCTTGGAAACCATTGAAAAAAAAATAGTGAGAAAACAAACACAATTTTCAAAACCCAAAAACTAAATGATTATCAAACGAGATCTAACTAGGGATGTTTATGAAAGAGTGAAGGCTTGCCAAAAGTAGAGTTGTTCAAGTTTCTTTTTTAGCTTCAACTCATAGAAAATTTCCTTTCTTTGGATTTTGACATGTCAAGGGTCATCTGGGTGCAACACATAAGCTAGAATATAGATTGGAAAGTAAAAGGACTAGGCAAAGAAACAATTTGTAACTAGGTTAATTTTTCAAAACAGGATTAAGGGCATGTTTGTGAGTGATTTTGAAATGACCAAAATCACTTTTTATCCCATCGAACATGCTCTAAATAAAACCGTTATTTAATGGGATTATAGGGATTAATGCAACGTTTATTTTGATTCAGGTACAGGTTCAAGGTTATTGTTGAGATGAAGAGATCAGCTTGTGGAGCAGTTTTGGAATGCTATGGAAACCCTCAAACAAGAAAGAAAGTAGCAGCAGAGCATGCAGCAGAAGGAGCATTATGGTACTTAAAGCATTTGGGTTATCGTTCTAAAAGATAATTAGGAATTGTAAAGAACTAGAAATGGAAAATGAATAAATCATTTTAACCCAAACTCTTTTCATGAAATCAAATTTTCATTAGCATGTAAAGGGTTTTTTTTTTGGGAGGGGGGAGAAAAAAATCAAAATTTTTTTTTCTTACGTTGTATAAATTTTTACCCTAAAATTTCAATTTTATCAAATTGCATTTCGAATTTAGATAAATGTTGCAATCTTAGTCTTAAACTTTAAAAAAAGTTACAACTTTAATCAATTGATAAGTTTTCGTTTGAAATATCGTTAAAAAAGTATTCTCATATGCATTCAGTGAATTCTAAAGCACATATATGATACAAAGTTTATAAAATCGAAAGAAAAATCACATTAGAGCTAAATTTTTGTTGAAATTTGTAAATTTTGGCAAGGTTATTCAATGGTTAATTTGTATTGACCAAACTTAATTTTGTACCAAGCTTGATTTTATTGAAATTAGTGAATTCATGGAGTTTTTTAAATTCCTTTTTGTTAAAATTAGGGGTTGAATTATCAGAAATTGAATAAAGGGTAAAATTTGTAATACTTATAAGTTTAGGTTCAATTTGATCGAATTTAAAATTTTTGAGTGAAAATTAATACGACTTAACAAGTATAAGAGTAAGATTTAGGACGGATATATGTTCATATTCTTTCTACTATCAATCTAAACCCCATCCAATCTTTTCTCTTACTCGATCTTTTCATCACTCAATCAAGTGGGTGTGTGGGTAGGTTCATGAGCCAAGTTGTTCATTAAGGATTGTAATAGCTAGTGCTAGCAGTGGCGGATCCAGTGAATCTCTGACTATAAGCTATTAACCACTATACCAGAAGACCTTTTCTATACATATTATTAAAGGTAAAAATTTGAGGGGGGTTCGAGCCCCTTGAGTCCATGGTAAATCCGTCCCTGGCTAGTGGTGAAATTTAAAGTTGAAGGACAAGTATAATTTTCACTTTAGATGATACTATTAGCTCGCAAAGTATTATATTATGTAAGTTTATCATAAACTAATTTTAATATTAAAATTTGACGGACTAAATTGGATAATATCCCAAAATTTTAAGAGGACAAAACATATATTTAAGCTAATTTAAACTCAAAATTTTATTATCGTATCGCATTTTTTAAACCAACTCAAACATTGTTCAAACTTCAAGGGTTAAGGCTCAAGACATTAATAACCTTTCTCAAATAACTCCAAAATTATATGTAATGATATATTTTAAAAAGATGTCTTCTTTTTCTAACAAAAAGAATTTGGTGGAATTAAATTGAGCGCTAAATTATAGAAAATACCCAAGGATTTCGAGTCTCATTTAGAACAAACTTTAAAAAGTATCATAAATAA

mRNA sequence

AATGGAGTTACTTTACTATAAACCAGATATAGAGAGAGAAGAAGAGGAGGAAGAAGAAGAAGAAGGAATTATGAAGAAGAAGTGAATTTGTTCAGAAGAGGTCACTAGCAGAGACAGTGCAGAGAGATCATTGGAGCCGAGCGGAAGAATCGAGGAAAGTAGATGGAGAATACACCTTCCTACACTCGAAGATTAAATGTCGATCTCAAAAAACTACCTCCAATTAATAATCCGCGCATTGGAAATGGAAATCACTCCTCGATTCCGGCTAAATTTGAGAAATCCAGGTATATTAGGCGTGTGCCTCAGTTTATGCCGTCGAATTACCGGCCGGAAATGAGGCCGAACGAATACGGTGGCTTGCCGATGAAACCTGTGCCTTCTGATTCCGCTCGAGCTTTGAATACGAAGACGATTCCGGAGGCTTCATCGGATTCCTCCGATAAAAAGAAAGAGAATAGCCGGGAAATAGCTTCGAGTTGCAGTTGCAGACATGGCTCGACCAAAGAAGGTACTCCAGATAAGAAATGGGCAAAGTCCCTTCTGTTTGAGATTTGCACTGCAAATCACTGGCAGCCTCCTCTGTTTGAATGCTGCGAGGAAGAAGGTCCAAGCCATGCAAGAAAGTACAGGTTCAAGGTTATTGTTGAGATGAAGAGATCAGCTTGTGGAGCAGTTTTGGAATGCTATGGAAACCCTCAAACAAGAAAGAAAGTAGCAGCAGAGCATGCAGCAGAAGGAGCATTATGGTACTTAAAGCATTTGGTCTCATTTAGAACAAACTTTAAAAAGTATCATAAATAA

Coding sequence (CDS)

ATGGAGAATACACCTTCCTACACTCGAAGATTAAATGTCGATCTCAAAAAACTACCTCCAATTAATAATCCGCGCATTGGAAATGGAAATCACTCCTCGATTCCGGCTAAATTTGAGAAATCCAGGTATATTAGGCGTGTGCCTCAGTTTATGCCGTCGAATTACCGGCCGGAAATGAGGCCGAACGAATACGGTGGCTTGCCGATGAAACCTGTGCCTTCTGATTCCGCTCGAGCTTTGAATACGAAGACGATTCCGGAGGCTTCATCGGATTCCTCCGATAAAAAGAAAGAGAATAGCCGGGAAATAGCTTCGAGTTGCAGTTGCAGACATGGCTCGACCAAAGAAGGTACTCCAGATAAGAAATGGGCAAAGTCCCTTCTGTTTGAGATTTGCACTGCAAATCACTGGCAGCCTCCTCTGTTTGAATGCTGCGAGGAAGAAGGTCCAAGCCATGCAAGAAAGTACAGGTTCAAGGTTATTGTTGAGATGAAGAGATCAGCTTGTGGAGCAGTTTTGGAATGCTATGGAAACCCTCAAACAAGAAAGAAAGTAGCAGCAGAGCATGCAGCAGAAGGAGCATTATGGTACTTAAAGCATTTGGTCTCATTTAGAACAAACTTTAAAAAGTATCATAAATAA

Protein sequence

MENTPSYTRRLNVDLKKLPPINNPRIGNGNHSSIPAKFEKSRYIRRVPQFMPSNYRPEMRPNEYGGLPMKPVPSDSARALNTKTIPEASSDSSDKKKENSREIASSCSCRHGSTKEGTPDKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLECYGNPQTRKKVAAEHAAEGALWYLKHLVSFRTNFKKYHK
Homology
BLAST of Tan0010267 vs. ExPASy Swiss-Prot
Match: P84634 (Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2)

HSP 1 Score: 105.9 bits (263), Expect = 5.7e-22
Identity = 49/86 (56.98%), Postives = 59/86 (68.60%), Query Frame = 0

Query: 115  KEGTPDKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLE 174
            K G P  K AKSLL E C AN W+PP FECCEEEGP H + + +KVI+E++  A    LE
Sbjct: 1613 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVE-DAPNMTLE 1672

Query: 175  CYGNPQTRKKVAAEHAAEGALWYLKH 201
            CYG  +  KK AAEHAA+ A+W LKH
Sbjct: 1673 CYGEARATKKGAAEHAAQAAIWCLKH 1697

BLAST of Tan0010267 vs. ExPASy Swiss-Prot
Match: Q9M8N2 (Ribonuclease 3-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RTL1 PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 5.7e-22
Identity = 66/203 (32.51%), Postives = 110/203 (54.19%), Query Frame = 0

Query: 4   TPSYTRRLNVDLKKLPPINNPRIGNGNHSSIPA--KFEKSRYIRRVPQFM-PSNYRPEMR 63
           T   +R + + LK +PP+        + SSIP+     +  +   + +F   ++++ E  
Sbjct: 10  TKKPSRSIIISLKDIPPL--------DPSSIPSMKPMAQDHHNVGMQRFQEKTDFKFEEE 69

Query: 64  PNEYGGLPMKPVPSDSAR--ALNTKTIPEASSDSSDKKKENSREIASSCSCRHGSTKEGT 123
            N         +  +S R  +L  K  P+   + +   K  S++  S        T+ G+
Sbjct: 70  DNAISSFSNIQIDPNSTRSISLEKKLAPKPDEEHTTTTKPISKDDES-------KTRRGS 129

Query: 124 PDKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLECYGN 183
                AKS+L E+C +  W+PP++ECC  +GP H R + +KV+VE++ S+   VLEC+G+
Sbjct: 130 -----AKSVLHEMCASKRWRPPVYECCNVDGPCHLRLFTYKVMVEIRDSSGKTVLECFGD 189

Query: 184 PQTRKKVAAEHAAEGALWYLKHL 202
           P+ +KK AAEHAAEGALWYL+H+
Sbjct: 190 PRRKKKAAAEHAAEGALWYLEHV 192

BLAST of Tan0010267 vs. ExPASy Swiss-Prot
Match: A7LFZ6 (Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL4 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 4.8e-21
Identity = 45/80 (56.25%), Postives = 61/80 (76.25%), Query Frame = 0

Query: 122  KWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLECYGNPQT 181
            K A+S LFE+C AN+W+PP F+ C+EEGPSH RK+ +KV+VE+K  A   +LEC+ + + 
Sbjct: 1568 KTARSFLFELCAANYWKPPEFKLCKEEGPSHLRKFTYKVVVEIK-GASATLLECHSDGKL 1627

Query: 182  RKKVAAEHAAEGALWYLKHL 202
            +KK A EHAA+GALW LK L
Sbjct: 1628 QKKAAQEHAAQGALWCLKQL 1646

BLAST of Tan0010267 vs. ExPASy Swiss-Prot
Match: Q8LMR2 (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL1 PE=3 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.4e-04
Identity = 28/82 (34.15%), Postives = 37/82 (45.12%), Query Frame = 0

Query: 110  RHGSTKEGTPDKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSAC 169
            R G  K G   + + +  L +IC    W  P + C  E GP+HA+  RF   V +  S  
Sbjct: 1786 RDGEKKNGA--QMFTRQTLNDICLRRQWPMPQYRCVNEGGPAHAK--RFVYSVRVNTSDR 1845

Query: 170  GAVLECYGNPQTRKKVAAEHAA 192
            G   EC G P    K A + AA
Sbjct: 1846 GWTDECIGEPMPSVKKAKDSAA 1863

BLAST of Tan0010267 vs. NCBI nr
Match: XP_038878793.1 (ribonuclease 3-like protein 1 isoform X3 [Benincasa hispida])

HSP 1 Score: 302.0 bits (772), Expect = 4.1e-78
Identity = 157/202 (77.72%), Postives = 169/202 (83.66%), Query Frame = 0

Query: 1   MENTPSYTRRLNVDLKKLPPINNPRIGNGNHSSIPAKFEKSRYIRRVPQFMPSNYRPEMR 60
           MENT S  RRLN +LK LPPINN  IG GNHSSI AKFEKSRYIRRVPQF+P N+    R
Sbjct: 1   MENTHSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRRVPQFIPQNH----R 60

Query: 61  PNEYGGLPMKPVPSDSARALNTKTIPEASSDSSDKKKENSREIAS-SCSCRHGSTKEGTP 120
           PNEY GLPMKP  S SARALN+KTIPEA SDS  +KK+NS+EIAS S  C HGSTKEGTP
Sbjct: 61  PNEYAGLPMKPACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASRSSCCGHGSTKEGTP 120

Query: 121 DKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLECYGNP 180
           +K+ AKSLLFEICTANHWQPPLFECCEEEGPSHA+KYRFKV VEMK  A   VLECYGN 
Sbjct: 121 EKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMK-GASETVLECYGNL 180

Query: 181 QTRKKVAAEHAAEGALWYLKHL 202
           QTRKKVAAEHAAEGA+WYLK+L
Sbjct: 181 QTRKKVAAEHAAEGAIWYLKNL 197

BLAST of Tan0010267 vs. NCBI nr
Match: XP_038878769.1 (ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida] >XP_038878776.1 ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 302.0 bits (772), Expect = 4.1e-78
Identity = 157/202 (77.72%), Postives = 169/202 (83.66%), Query Frame = 0

Query: 1   MENTPSYTRRLNVDLKKLPPINNPRIGNGNHSSIPAKFEKSRYIRRVPQFMPSNYRPEMR 60
           MENT S  RRLN +LK LPPINN  IG GNHSSI AKFEKSRYIRRVPQF+P N+    R
Sbjct: 18  MENTHSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRRVPQFIPQNH----R 77

Query: 61  PNEYGGLPMKPVPSDSARALNTKTIPEASSDSSDKKKENSREIAS-SCSCRHGSTKEGTP 120
           PNEY GLPMKP  S SARALN+KTIPEA SDS  +KK+NS+EIAS S  C HGSTKEGTP
Sbjct: 78  PNEYAGLPMKPACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASRSSCCGHGSTKEGTP 137

Query: 121 DKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLECYGNP 180
           +K+ AKSLLFEICTANHWQPPLFECCEEEGPSHA+KYRFKV VEMK  A   VLECYGN 
Sbjct: 138 EKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMK-GASETVLECYGNL 197

Query: 181 QTRKKVAAEHAAEGALWYLKHL 202
           QTRKKVAAEHAAEGA+WYLK+L
Sbjct: 198 QTRKKVAAEHAAEGAIWYLKNL 214

BLAST of Tan0010267 vs. NCBI nr
Match: XP_004152191.1 (uncharacterized protein LOC101212514 [Cucumis sativus] >KGN52916.1 hypothetical protein Csa_014441 [Cucumis sativus])

HSP 1 Score: 300.8 bits (769), Expect = 9.2e-78
Identity = 154/204 (75.49%), Postives = 173/204 (84.80%), Query Frame = 0

Query: 1   MENTPSY-TRRLNVDLKKLPPINNPRIGNGNHSSIPAKFEKSRYIRRVPQFMPSNYRPEM 60
           MEN PS    RLN++LK LPPINNPR  +G+HSSIPAKFEKSRYIRRVPQFMPS++RPE+
Sbjct: 19  MENNPSCPPPRLNINLKNLPPINNPRNVSGSHSSIPAKFEKSRYIRRVPQFMPSDHRPEI 78

Query: 61  RPNEYGGLPMKPVPSDSARALNTKTIPEA-SSDSSDKKKENSREIASSCS-CRHGSTKEG 120
           R N+Y GLPMK   SDSARALNTKT P A S+DS  +KK+  +++AS CS CRH STKEG
Sbjct: 79  RSNDYAGLPMKLAYSDSARALNTKTFPVAPSADSFVRKKDTCKDLASRCSCCRHDSTKEG 138

Query: 121 TPDKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLECYG 180
           TP+K+ AKSLLFEICTANHWQPPLFECCEEEGPSHA+KYRFKV +EMK   C AV+ECYG
Sbjct: 139 TPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMK-GDCEAVVECYG 198

Query: 181 NPQTRKKVAAEHAAEGALWYLKHL 202
           N QTRKKVAAEHAAEGALWYL HL
Sbjct: 199 NLQTRKKVAAEHAAEGALWYLNHL 221

BLAST of Tan0010267 vs. NCBI nr
Match: XP_038878786.1 (ribonuclease 3-like protein 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 294.3 bits (752), Expect = 8.6e-76
Identity = 153/197 (77.66%), Postives = 164/197 (83.25%), Query Frame = 0

Query: 1   MENTPSYTRRLNVDLKKLPPINNPRIGNGNHSSIPAKFEKSRYIRRVPQFMPSNYRPEMR 60
           MENT S  RRLN +LK LPPINN  IG GNHSSI AKFEKSRYIRRVPQF+P N+    R
Sbjct: 18  MENTHSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRRVPQFIPQNH----R 77

Query: 61  PNEYGGLPMKPVPSDSARALNTKTIPEASSDSSDKKKENSREIAS-SCSCRHGSTKEGTP 120
           PNEY GLPMKP  S SARALN+KTIPEA SDS  +KK+NS+EIAS S  C HGSTKEGTP
Sbjct: 78  PNEYAGLPMKPACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASRSSCCGHGSTKEGTP 137

Query: 121 DKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLECYGNP 180
           +K+ AKSLLFEICTANHWQPPLFECCEEEGPSHA+KYRFKV VEMK  A   VLECYGN 
Sbjct: 138 EKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMK-GASETVLECYGNL 197

Query: 181 QTRKKVAAEHAAEGALW 197
           QTRKKVAAEHAAEGA+W
Sbjct: 198 QTRKKVAAEHAAEGAIW 209

BLAST of Tan0010267 vs. NCBI nr
Match: XP_008454206.1 (PREDICTED: uncharacterized protein LOC103494682 isoform X1 [Cucumis melo] >KAA0044459.1 ribonuclease 3-like protein 1 [Cucumis melo var. makuwa] >TYK29586.1 ribonuclease 3-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 293.9 bits (751), Expect = 1.1e-75
Identity = 151/203 (74.38%), Postives = 171/203 (84.24%), Query Frame = 0

Query: 2   ENTPSYTRRLNVDLKKLPPINNPRIGNGNHSSIPAKFEKSRYIRRVPQFMPSNYRPEMRP 61
           +N PS   RLN++LK LPPINNPR   G HSSIPAKFEKSRYIRRVPQF+P ++RPE+R 
Sbjct: 21  KNNPSCPPRLNINLKNLPPINNPRNVTGTHSSIPAKFEKSRYIRRVPQFIPFDHRPEIRS 80

Query: 62  NEYGGLP-MKPVPSDSARALNTKTIPEA-SSDSSDKKKENSREIASSCS-CRHGSTKEGT 121
           N Y GLP MK   SDSARA+NTKTIP A S+D   +KK+N +++AS CS CRHGSTKEGT
Sbjct: 81  NNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKDNCKDLASRCSCCRHGSTKEGT 140

Query: 122 PDKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLECYGN 181
           P+K+ AKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKV +EMK   C AV+ECYGN
Sbjct: 141 PEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVSIEMK-GDCEAVVECYGN 200

Query: 182 PQTRKKVAAEHAAEGALWYLKHL 202
            QTRKK+AAEHAAEGALWYLK+L
Sbjct: 201 LQTRKKMAAEHAAEGALWYLKNL 222

BLAST of Tan0010267 vs. ExPASy TrEMBL
Match: A0A0A0KYI7 (DRBM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006230 PE=4 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 4.4e-78
Identity = 154/204 (75.49%), Postives = 173/204 (84.80%), Query Frame = 0

Query: 1   MENTPSY-TRRLNVDLKKLPPINNPRIGNGNHSSIPAKFEKSRYIRRVPQFMPSNYRPEM 60
           MEN PS    RLN++LK LPPINNPR  +G+HSSIPAKFEKSRYIRRVPQFMPS++RPE+
Sbjct: 19  MENNPSCPPPRLNINLKNLPPINNPRNVSGSHSSIPAKFEKSRYIRRVPQFMPSDHRPEI 78

Query: 61  RPNEYGGLPMKPVPSDSARALNTKTIPEA-SSDSSDKKKENSREIASSCS-CRHGSTKEG 120
           R N+Y GLPMK   SDSARALNTKT P A S+DS  +KK+  +++AS CS CRH STKEG
Sbjct: 79  RSNDYAGLPMKLAYSDSARALNTKTFPVAPSADSFVRKKDTCKDLASRCSCCRHDSTKEG 138

Query: 121 TPDKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLECYG 180
           TP+K+ AKSLLFEICTANHWQPPLFECCEEEGPSHA+KYRFKV +EMK   C AV+ECYG
Sbjct: 139 TPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMK-GDCEAVVECYG 198

Query: 181 NPQTRKKVAAEHAAEGALWYLKHL 202
           N QTRKKVAAEHAAEGALWYL HL
Sbjct: 199 NLQTRKKVAAEHAAEGALWYLNHL 221

BLAST of Tan0010267 vs. ExPASy TrEMBL
Match: A0A5D3E0Z9 (Ribonuclease 3-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001930 PE=4 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 5.4e-76
Identity = 151/203 (74.38%), Postives = 171/203 (84.24%), Query Frame = 0

Query: 2   ENTPSYTRRLNVDLKKLPPINNPRIGNGNHSSIPAKFEKSRYIRRVPQFMPSNYRPEMRP 61
           +N PS   RLN++LK LPPINNPR   G HSSIPAKFEKSRYIRRVPQF+P ++RPE+R 
Sbjct: 21  KNNPSCPPRLNINLKNLPPINNPRNVTGTHSSIPAKFEKSRYIRRVPQFIPFDHRPEIRS 80

Query: 62  NEYGGLP-MKPVPSDSARALNTKTIPEA-SSDSSDKKKENSREIASSCS-CRHGSTKEGT 121
           N Y GLP MK   SDSARA+NTKTIP A S+D   +KK+N +++AS CS CRHGSTKEGT
Sbjct: 81  NNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKDNCKDLASRCSCCRHGSTKEGT 140

Query: 122 PDKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLECYGN 181
           P+K+ AKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKV +EMK   C AV+ECYGN
Sbjct: 141 PEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVSIEMK-GDCEAVVECYGN 200

Query: 182 PQTRKKVAAEHAAEGALWYLKHL 202
            QTRKK+AAEHAAEGALWYLK+L
Sbjct: 201 LQTRKKMAAEHAAEGALWYLKNL 222

BLAST of Tan0010267 vs. ExPASy TrEMBL
Match: A0A1S3BXK0 (uncharacterized protein LOC103494682 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494682 PE=4 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 5.4e-76
Identity = 151/203 (74.38%), Postives = 171/203 (84.24%), Query Frame = 0

Query: 2   ENTPSYTRRLNVDLKKLPPINNPRIGNGNHSSIPAKFEKSRYIRRVPQFMPSNYRPEMRP 61
           +N PS   RLN++LK LPPINNPR   G HSSIPAKFEKSRYIRRVPQF+P ++RPE+R 
Sbjct: 21  KNNPSCPPRLNINLKNLPPINNPRNVTGTHSSIPAKFEKSRYIRRVPQFIPFDHRPEIRS 80

Query: 62  NEYGGLP-MKPVPSDSARALNTKTIPEA-SSDSSDKKKENSREIASSCS-CRHGSTKEGT 121
           N Y GLP MK   SDSARA+NTKTIP A S+D   +KK+N +++AS CS CRHGSTKEGT
Sbjct: 81  NNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKDNCKDLASRCSCCRHGSTKEGT 140

Query: 122 PDKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLECYGN 181
           P+K+ AKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKV +EMK   C AV+ECYGN
Sbjct: 141 PEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVSIEMK-GDCEAVVECYGN 200

Query: 182 PQTRKKVAAEHAAEGALWYLKHL 202
            QTRKK+AAEHAAEGALWYLK+L
Sbjct: 201 LQTRKKMAAEHAAEGALWYLKNL 222

BLAST of Tan0010267 vs. ExPASy TrEMBL
Match: A0A6J1DJ12 (uncharacterized protein LOC111020939 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020939 PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 1.3e-72
Identity = 148/202 (73.27%), Postives = 162/202 (80.20%), Query Frame = 0

Query: 1   MENTPSYTRRLNVDLKKLPPINNPRIGNGNHS-SIPAKFEKSRYIRRVPQFMPSNYRPEM 60
           ME TP Y RRLNV+LK LPPI NPR GNGNH  SIPAKFEKSRYIRRVP F P N+RPEM
Sbjct: 1   MEQTPPYPRRLNVNLKNLPPI-NPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEM 60

Query: 61  RPNEYGGLPMKPVPSDSARALNTKTIPEASSDSSDKKKENSREIASSCSCRHGSTKEGTP 120
           +P             D+A ALNTKTIPEASSDS   K ENSREIAS+CSC H S KEGT 
Sbjct: 61  KP-------------DTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTL 120

Query: 121 DKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLECYGNP 180
           +K+ AKSLLFE+CTAN+W+PPLFECCEEEGPSHA+K+RFKVIVEMK  AC AVLECYGN 
Sbjct: 121 EKRAAKSLLFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMK-GACDAVLECYGNS 180

Query: 181 QTRKKVAAEHAAEGALWYLKHL 202
           Q RKKVAA++AAEGALW LKHL
Sbjct: 181 QARKKVAAQNAAEGALWCLKHL 187

BLAST of Tan0010267 vs. ExPASy TrEMBL
Match: A0A6J1GWD5 (ribonuclease 3-like protein 1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111458030 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 1.4e-71
Identity = 141/202 (69.80%), Postives = 159/202 (78.71%), Query Frame = 0

Query: 1   MENTPSYTRRLNVDLKKLPPINNPRIGNGNHSSIPAKFEKSRYIRRVPQFMPSNYRPEMR 60
           MENTPS  R +NV+LK LPPI+NP +GN NHSSIPAKFEKSRYI RVPQFMP N+ P++R
Sbjct: 1   MENTPSCPRSVNVNLKNLPPIHNPCVGNANHSSIPAKFEKSRYIGRVPQFMPPNHSPDVR 60

Query: 61  PNEYGGLPMKPVPSDSARALNTKTIPEASSDSSDKKKENSREIAS-SCSCRHGSTKEGTP 120
           PNEY G+PM P   DSAR+ N +T P+       ++KEN  + AS S  C H STKEGT 
Sbjct: 61  PNEYDGVPMNPACFDSARSPNARTTPK-------ERKENFGDSASRSSGCSHDSTKEGTS 120

Query: 121 DKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLECYGNP 180
            KK AKSLL+EICTANHW+PPLFECCEEEGPSHA+KY FKVIVEMK   CG  LECYG P
Sbjct: 121 VKKSAKSLLYEICTANHWKPPLFECCEEEGPSHAKKYTFKVIVEMK-GDCGEDLECYGKP 180

Query: 181 QTRKKVAAEHAAEGALWYLKHL 202
           QTRKKVAAE AAEGA+WYLKHL
Sbjct: 181 QTRKKVAAEDAAEGAVWYLKHL 194

BLAST of Tan0010267 vs. TAIR 10
Match: AT1G80650.1 (RNAse THREE-like protein 1 )

HSP 1 Score: 105.9 bits (263), Expect = 4.0e-23
Identity = 66/203 (32.51%), Postives = 110/203 (54.19%), Query Frame = 0

Query: 4   TPSYTRRLNVDLKKLPPINNPRIGNGNHSSIPA--KFEKSRYIRRVPQFM-PSNYRPEMR 63
           T   +R + + LK +PP+        + SSIP+     +  +   + +F   ++++ E  
Sbjct: 10  TKKPSRSIIISLKDIPPL--------DPSSIPSMKPMAQDHHNVGMQRFQEKTDFKFEEE 69

Query: 64  PNEYGGLPMKPVPSDSAR--ALNTKTIPEASSDSSDKKKENSREIASSCSCRHGSTKEGT 123
            N         +  +S R  +L  K  P+   + +   K  S++  S        T+ G+
Sbjct: 70  DNAISSFSNIQIDPNSTRSISLEKKLAPKPDEEHTTTTKPISKDDES-------KTRRGS 129

Query: 124 PDKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLECYGN 183
                AKS+L E+C +  W+PP++ECC  +GP H R + +KV+VE++ S+   VLEC+G+
Sbjct: 130 -----AKSVLHEMCASKRWRPPVYECCNVDGPCHLRLFTYKVMVEIRDSSGKTVLECFGD 189

Query: 184 PQTRKKVAAEHAAEGALWYLKHL 202
           P+ +KK AAEHAAEGALWYL+H+
Sbjct: 190 PRRKKKAAAEHAAEGALWYLEHV 192

BLAST of Tan0010267 vs. TAIR 10
Match: AT5G20320.1 (dicer-like 4 )

HSP 1 Score: 105.9 bits (263), Expect = 4.0e-23
Identity = 49/86 (56.98%), Postives = 59/86 (68.60%), Query Frame = 0

Query: 115  KEGTPDKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLE 174
            K G P  K AKSLL E C AN W+PP FECCEEEGP H + + +KVI+E++  A    LE
Sbjct: 1613 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVE-DAPNMTLE 1672

Query: 175  CYGNPQTRKKVAAEHAAEGALWYLKH 201
            CYG  +  KK AAEHAA+ A+W LKH
Sbjct: 1673 CYGEARATKKGAAEHAAQAAIWCLKH 1697

BLAST of Tan0010267 vs. TAIR 10
Match: AT5G20320.2 (dicer-like 4 )

HSP 1 Score: 105.9 bits (263), Expect = 4.0e-23
Identity = 49/86 (56.98%), Postives = 59/86 (68.60%), Query Frame = 0

Query: 115  KEGTPDKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVLE 174
            K G P  K AKSLL E C AN W+PP FECCEEEGP H + + +KVI+E++  A    LE
Sbjct: 1599 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVE-DAPNMTLE 1658

Query: 175  CYGNPQTRKKVAAEHAAEGALWYLKH 201
            CYG  +  KK AAEHAA+ A+W LKH
Sbjct: 1659 CYGEARATKKGAAEHAAQAAIWCLKH 1683

BLAST of Tan0010267 vs. TAIR 10
Match: AT4G00420.2 (Double-stranded RNA-binding domain (DsRBD)-containing protein )

HSP 1 Score: 92.4 bits (228), Expect = 4.6e-19
Identity = 44/88 (50.00%), Postives = 58/88 (65.91%), Query Frame = 0

Query: 114 TKEGTPDKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVL 173
           T E    +  AKS L+ +C+  HW+ PL+E    EGP H + +  KV VEMK  +   VL
Sbjct: 75  TTEEETQRSSAKSQLYNLCSVRHWKAPLYEYI-AEGPCHMKIFTGKVTVEMKEDSRITVL 134

Query: 174 ECYGNPQTRKKVAAEHAAEGALWYLKHL 202
           EC+GNPQ +KK+AAE AAE ALWYLK++
Sbjct: 135 ECFGNPQYKKKIAAEQAAEAALWYLKNV 161

BLAST of Tan0010267 vs. TAIR 10
Match: AT4G00420.3 (Double-stranded RNA-binding domain (DsRBD)-containing protein )

HSP 1 Score: 92.4 bits (228), Expect = 4.6e-19
Identity = 44/88 (50.00%), Postives = 58/88 (65.91%), Query Frame = 0

Query: 114 TKEGTPDKKWAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVIVEMKRSACGAVL 173
           T E    +  AKS L+ +C+  HW+ PL+E    EGP H + +  KV VEMK  +   VL
Sbjct: 53  TTEEETQRSSAKSQLYNLCSVRHWKAPLYEYI-AEGPCHMKIFTGKVTVEMKEDSRITVL 112

Query: 174 ECYGNPQTRKKVAAEHAAEGALWYLKHL 202
           EC+GNPQ +KK+AAE AAE ALWYLK++
Sbjct: 113 ECFGNPQYKKKIAAEQAAEAALWYLKNV 139

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P846345.7e-2256.98Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2[more]
Q9M8N25.7e-2232.51Ribonuclease 3-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RTL1 PE=2 SV=1[more]
A7LFZ64.8e-2156.25Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL... [more]
Q8LMR21.4e-0434.15Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL... [more]
Match NameE-valueIdentityDescription
XP_038878793.14.1e-7877.72ribonuclease 3-like protein 1 isoform X3 [Benincasa hispida][more]
XP_038878769.14.1e-7877.72ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida] >XP_038878776.1 rib... [more]
XP_004152191.19.2e-7875.49uncharacterized protein LOC101212514 [Cucumis sativus] >KGN52916.1 hypothetical ... [more]
XP_038878786.18.6e-7677.66ribonuclease 3-like protein 1 isoform X2 [Benincasa hispida][more]
XP_008454206.11.1e-7574.38PREDICTED: uncharacterized protein LOC103494682 isoform X1 [Cucumis melo] >KAA00... [more]
Match NameE-valueIdentityDescription
A0A0A0KYI74.4e-7875.49DRBM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006230 PE=4 S... [more]
A0A5D3E0Z95.4e-7674.38Ribonuclease 3-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3BXK05.4e-7674.38uncharacterized protein LOC103494682 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1DJ121.3e-7273.27uncharacterized protein LOC111020939 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1GWD51.4e-7169.80ribonuclease 3-like protein 1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
Match NameE-valueIdentityDescription
AT1G80650.14.0e-2332.51RNAse THREE-like protein 1 [more]
AT5G20320.14.0e-2356.98dicer-like 4 [more]
AT5G20320.24.0e-2356.98dicer-like 4 [more]
AT4G00420.24.6e-1950.00Double-stranded RNA-binding domain (DsRBD)-containing protein [more]
AT4G00420.34.6e-1950.00Double-stranded RNA-binding domain (DsRBD)-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014720Double-stranded RNA-binding domainSMARTSM00358DRBM_3coord: 74..149
e-value: 1.7E-6
score: 37.6
IPR014720Double-stranded RNA-binding domainPROSITEPS50137DS_RBDcoord: 73..150
score: 9.003672
NoneNo IPR availablePFAMPF14709DND1_DSRMcoord: 74..149
e-value: 3.1E-15
score: 56.2
NoneNo IPR availableGENE3D3.30.160.20coord: 80..150
e-value: 2.2E-22
score: 81.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 39..54
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..69
NoneNo IPR availablePANTHERPTHR14950DICER-RELATEDcoord: 1..157
NoneNo IPR availablePANTHERPTHR14950:SF67DOUBLE-STRANDED RNA-BINDING DOMAIN (DSRBD)-CONTAINING PROTEINcoord: 1..157
NoneNo IPR availableSUPERFAMILY54768dsRNA-binding domain-likecoord: 72..153

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0010267.1Tan0010267.1mRNA
Tan0010267.2Tan0010267.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030422 production of siRNA involved in RNA interference
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0004525 ribonuclease III activity
molecular_function GO:0003723 RNA binding