Tan0010176 (gene) Snake gourd v1

Overview
NameTan0010176
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionprotein GAMETE EXPRESSED 1
LocationLG04: 77997025 .. 77999167 (-)
RNA-Seq ExpressionTan0010176
SyntenyTan0010176
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCCTGTTCTGTTCATGGTGATTCTGTTATTTGTGTTGTCTTCTTCATCTTCGGACTCTACCACGAATCCTTCTAAGCATTACAGTGTAGCAGAATTTTCCATGGAGGGTTTCGATGATCGAAAGGGGGTGAAGCGAATTGAGAATGCTAAGAACAAATTGACTGTGTCTAATTCTTGCTGGGAAAGTGCTTATAGGCATTTATTTGTGGGCTGCTCTGAGATTTTTGCTGTGGATGAGAAACGGTGGAGATTCGCTTGGCATCTTACCGATTGCTTTCAGAAGGATTCTGGTAGGCCTCCTTTTCCTTCCTGTGACCCGGAATCCGCCATGGCCGAATGCCTCAAATACTTGAATGAACATGAGCATAGGATCTATCTCGAATTCTACCTTGAAACTAACTCCATTTGTCATCAGCTTCAGTGAGTATCATATCGTATCTTATTTTTCGTCTAATCGTGTTTACTTTTTCTTTCTTATGAAAATTAACATTATCTCCGTGGTCTTGTTTCTAGGGCTCATGCTTTCAAGCACGAAACGGAGAGACTGGTGAATGAGTTGAAGAGATCTTCGGAATCTGCAGAAGCCAAGTTAGAAATCATTGAGGAAAGATCGGAGTCTCTGTTGCAAAATTCATATCACATTTTTGATTCCTTGAATTCTGCTGGTATTCAAATACAACAAATGGCTCAAACATCGAGGAAACTAGAAGATGGAATGGGCGATGTGCTAAAGCGATCAGAAGATGTTTATGAGCAATCCAGGAGGATAGAGACATCCCAGTCAGAACTTCAAGAAGGTCAGTCAAAACTTAGAAGAAGTTTGGAGGAAGGGATGGAAATGCTTCAGGATTCCTACAAGGATCTTGGTCGAGAGATGGACGGTTTAAGAGACGGAGCCGTTGAGATCGAGAAGGAGATAACCAAAGTCGGAGATGCAATGTTTTCGAAGATGACATACTTGCAGAGCACGGCTGATGATATTGGGAATATGGCAGGGCTTTCATTGGATAAACAACAAGAACTTCTCGATGCTCAATCGATAGCGCTCAATGGTCTTCGTTCTTTAAGCAAAGTTCAATCTGAAGCGCTAGAGGAAAGCAGGTAAATAAAATTATGGAGATAAAGAGTAAATTTAGAACCTGCTTAAAGTTTCTCTAAATTGCTTTGTGACCTGTAGGAGTAAGCTGCAACAGTTGGCTGAATATGGCCACAGACAGCAAGAGGAGCTTCTTCAACGACAGAAACAGCTTCAACAATTTCATGATCGTTTAATGGAAAATTCTCAGTCAATCATGGCAGCTCAGGTAAGCCTTTTCTTTAGCCAAGGCTATTTGGGAGTGAATCTATTGCTTACTTTTTAACTCGGTTGTCAGCAATCATTTGAATCAAAGCAAGCAAGCATATTCATTGCGTTAGACAAGCTCTTCACCTTGCACAATGCAATGTTGCTTGAATCTCGATTAATCAAAGTCTTCTTCATTTACTTCACTTCAATCTTCATCATCTACATGTTTAGTAGCACCAAGCAAACTTACGCTGTTAGGCCCTGGCTATATATTGGTAATACAATATTCTGTTCAACAAATCTTCTCCTTCTTTGTAGGTCGGATAGATTACTGATCAAATCAATTTATAATTTCCAGAGCTGTGTGCTACATTCATATTTGAAGTAGCAATACTTCGGTTGGAGGTGTTTGACATAGAACAGCAAACATGGATCATAAATTTGGTTCGGATAGTCTTCCTGTTTCTTGCTTTTTTTCAGCTTCTTCATGCCATTTTCACATACAGGTAAATCAACATATAATTTTACAAATGAAACCTTAGTCATATGTAAATGCACTTGTTGAGGGGATAATTAATACAATTCGATGCCATCAAAATGCAGAGACTACGATGCCTTGAACTATCACATGCTACTAATGTTAACTGAAAGGATCAATGAGATGCAGACACCAAATAAGTTATCTTGGGGCAGTGACAGTGATTTGAACTGGTCATCGTGGATTGACACTGAGTTATCGGAAGATGTTGAAGATCTTGATTATGTTCTTCCAGAGGAAATAGGAGAGAATTCAATCACAACTACTTCAACAACAAGAAAGTACAATCTTCGCCGTCGACCCCTTCGTCAGTAA

mRNA sequence

ATGGAGCCTGTTCTGTTCATGGTGATTCTGTTATTTGTGTTGTCTTCTTCATCTTCGGACTCTACCACGAATCCTTCTAAGCATTACAGTGTAGCAGAATTTTCCATGGAGGGTTTCGATGATCGAAAGGGGGTGAAGCGAATTGAGAATGCTAAGAACAAATTGACTGTGTCTAATTCTTGCTGGGAAAGTGCTTATAGGCATTTATTTGTGGGCTGCTCTGAGATTTTTGCTGTGGATGAGAAACGGTGGAGATTCGCTTGGCATCTTACCGATTGCTTTCAGAAGGATTCTGGTAGGCCTCCTTTTCCTTCCTGTGACCCGGAATCCGCCATGGCCGAATGCCTCAAATACTTGAATGAACATGAGCATAGGATCTATCTCGAATTCTACCTTGAAACTAACTCCATTTGTCATCAGCTTCAGGCTCATGCTTTCAAGCACGAAACGGAGAGACTGGTGAATGAGTTGAAGAGATCTTCGGAATCTGCAGAAGCCAAGTTAGAAATCATTGAGGAAAGATCGGAGTCTCTGTTGCAAAATTCATATCACATTTTTGATTCCTTGAATTCTGCTGGTATTCAAATACAACAAATGGCTCAAACATCGAGGAAACTAGAAGATGGAATGGGCGATGTGCTAAAGCGATCAGAAGATGTTTATGAGCAATCCAGGAGGATAGAGACATCCCAGTCAGAACTTCAAGAAGGTCAGTCAAAACTTAGAAGAAGTTTGGAGGAAGGGATGGAAATGCTTCAGGATTCCTACAAGGATCTTGGTCGAGAGATGGACGGTTTAAGAGACGGAGCCGTTGAGATCGAGAAGGAGATAACCAAAGTCGGAGATGCAATGTTTTCGAAGATGACATACTTGCAGAGCACGGCTGATGATATTGGGAATATGGCAGGGCTTTCATTGGATAAACAACAAGAACTTCTCGATGCTCAATCGATAGCGCTCAATGGTCTTCGTTCTTTAAGCAAAGTTCAATCTGAAGCGCTAGAGGAAAGCAGGAGTAAGCTGCAACAGTTGGCTGAATATGGCCACAGACAGCAAGAGGAGCTTCTTCAACGACAGAAACAGCTTCAACAATTTCATGATCGTTTAATGGAAAATTCTCAGTCAATCATGGCAGCTCAGCAATCATTTGAATCAAAGCAAGCAAGCATATTCATTGCGTTAGACAAGCTCTTCACCTTGCACAATGCAATGTTGCTTGAATCTCGATTAATCAAAGTCTTCTTCATTTACTTCACTTCAATCTTCATCATCTACATGTTTAGTAGCACCAAGCAAACTTACGCTGTTAGGCCCTGGCTATATATTGAGCTGTGTGCTACATTCATATTTGAAGTAGCAATACTTCGGTTGGAGGTGTTTGACATAGAACAGCAAACATGGATCATAAATTTGGTTCGGATAGTCTTCCTGTTTCTTGCTTTTTTTCAGCTTCTTCATGCCATTTTCACATACAGAGACTACGATGCCTTGAACTATCACATGCTACTAATGTTAACTGAAAGGATCAATGAGATGCAGACACCAAATAAGTTATCTTGGGGCAGTGACAGTGATTTGAACTGGTCATCGTGGATTGACACTGAGTTATCGGAAGATGTTGAAGATCTTGATTATGTTCTTCCAGAGGAAATAGGAGAGAATTCAATCACAACTACTTCAACAACAAGAAAGTACAATCTTCGCCGTCGACCCCTTCGTCAGTAA

Coding sequence (CDS)

ATGGAGCCTGTTCTGTTCATGGTGATTCTGTTATTTGTGTTGTCTTCTTCATCTTCGGACTCTACCACGAATCCTTCTAAGCATTACAGTGTAGCAGAATTTTCCATGGAGGGTTTCGATGATCGAAAGGGGGTGAAGCGAATTGAGAATGCTAAGAACAAATTGACTGTGTCTAATTCTTGCTGGGAAAGTGCTTATAGGCATTTATTTGTGGGCTGCTCTGAGATTTTTGCTGTGGATGAGAAACGGTGGAGATTCGCTTGGCATCTTACCGATTGCTTTCAGAAGGATTCTGGTAGGCCTCCTTTTCCTTCCTGTGACCCGGAATCCGCCATGGCCGAATGCCTCAAATACTTGAATGAACATGAGCATAGGATCTATCTCGAATTCTACCTTGAAACTAACTCCATTTGTCATCAGCTTCAGGCTCATGCTTTCAAGCACGAAACGGAGAGACTGGTGAATGAGTTGAAGAGATCTTCGGAATCTGCAGAAGCCAAGTTAGAAATCATTGAGGAAAGATCGGAGTCTCTGTTGCAAAATTCATATCACATTTTTGATTCCTTGAATTCTGCTGGTATTCAAATACAACAAATGGCTCAAACATCGAGGAAACTAGAAGATGGAATGGGCGATGTGCTAAAGCGATCAGAAGATGTTTATGAGCAATCCAGGAGGATAGAGACATCCCAGTCAGAACTTCAAGAAGGTCAGTCAAAACTTAGAAGAAGTTTGGAGGAAGGGATGGAAATGCTTCAGGATTCCTACAAGGATCTTGGTCGAGAGATGGACGGTTTAAGAGACGGAGCCGTTGAGATCGAGAAGGAGATAACCAAAGTCGGAGATGCAATGTTTTCGAAGATGACATACTTGCAGAGCACGGCTGATGATATTGGGAATATGGCAGGGCTTTCATTGGATAAACAACAAGAACTTCTCGATGCTCAATCGATAGCGCTCAATGGTCTTCGTTCTTTAAGCAAAGTTCAATCTGAAGCGCTAGAGGAAAGCAGGAGTAAGCTGCAACAGTTGGCTGAATATGGCCACAGACAGCAAGAGGAGCTTCTTCAACGACAGAAACAGCTTCAACAATTTCATGATCGTTTAATGGAAAATTCTCAGTCAATCATGGCAGCTCAGCAATCATTTGAATCAAAGCAAGCAAGCATATTCATTGCGTTAGACAAGCTCTTCACCTTGCACAATGCAATGTTGCTTGAATCTCGATTAATCAAAGTCTTCTTCATTTACTTCACTTCAATCTTCATCATCTACATGTTTAGTAGCACCAAGCAAACTTACGCTGTTAGGCCCTGGCTATATATTGAGCTGTGTGCTACATTCATATTTGAAGTAGCAATACTTCGGTTGGAGGTGTTTGACATAGAACAGCAAACATGGATCATAAATTTGGTTCGGATAGTCTTCCTGTTTCTTGCTTTTTTTCAGCTTCTTCATGCCATTTTCACATACAGAGACTACGATGCCTTGAACTATCACATGCTACTAATGTTAACTGAAAGGATCAATGAGATGCAGACACCAAATAAGTTATCTTGGGGCAGTGACAGTGATTTGAACTGGTCATCGTGGATTGACACTGAGTTATCGGAAGATGTTGAAGATCTTGATTATGTTCTTCCAGAGGAAATAGGAGAGAATTCAATCACAACTACTTCAACAACAAGAAAGTACAATCTTCGCCGTCGACCCCTTCGTCAGTAA

Protein sequence

MEPVLFMVILLFVLSSSSSDSTTNPSKHYSVAEFSMEGFDDRKGVKRIENAKNKLTVSNSCWESAYRHLFVGCSEIFAVDEKRWRFAWHLTDCFQKDSGRPPFPSCDPESAMAECLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHETERLVNELKRSSESAEAKLEIIEERSESLLQNSYHIFDSLNSAGIQIQQMAQTSRKLEDGMGDVLKRSEDVYEQSRRIETSQSELQEGQSKLRRSLEEGMEMLQDSYKDLGREMDGLRDGAVEIEKEITKVGDAMFSKMTYLQSTADDIGNMAGLSLDKQQELLDAQSIALNGLRSLSKVQSEALEESRSKLQQLAEYGHRQQEELLQRQKQLQQFHDRLMENSQSIMAAQQSFESKQASIFIALDKLFTLHNAMLLESRLIKVFFIYFTSIFIIYMFSSTKQTYAVRPWLYIELCATFIFEVAILRLEVFDIEQQTWIINLVRIVFLFLAFFQLLHAIFTYRDYDALNYHMLLMLTERINEMQTPNKLSWGSDSDLNWSSWIDTELSEDVEDLDYVLPEEIGENSITTTSTTRKYNLRRRPLRQ
Homology
BLAST of Tan0010176 vs. ExPASy Swiss-Prot
Match: Q681K7 (Protein GAMETE EXPRESSED 1 OS=Arabidopsis thaliana OX=3702 GN=GEX1 PE=1 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 6.4e-145
Identity = 284/567 (50.09%), Postives = 406/567 (71.60%), Query Frame = 0

Query: 16  SSSSDSTTNP------SKHYSVAEFSMEGFDDRKGVKRIENAKNKLTVSNSCWESAYRHL 75
           SSSS S  +P          S  +FSME F D+K V+ +E   NKL    SCW++AY +L
Sbjct: 31  SSSSSSAEDPYSSSFSRSRKSNPDFSMEVFSDQKAVQVLE---NKLVGLTSCWQNAYSYL 90

Query: 76  FVGCSEIFAVDEKRWRFAWHLTDCFQKDSGRPPFPSCDPESAMAECLKYLNEHEHRIYLE 135
             GC E  A +EKR RFAW+L+DCF KDSGRP FP+C  ES M  CLK L++HEH+IYL+
Sbjct: 91  LAGCKETIATEEKRKRFAWYLSDCFIKDSGRPAFPTCKDESVMMSCLKKLDDHEHKIYLD 150

Query: 136 FYLETNSICHQLQAHAFKHETERLVNELKRSSESAEAKLEIIEERSESLLQNSYHIFDSL 195
           F LETN+IC QLQ++AFK+E ERLVNELK +++  E KL+I+E +S++L+Q S  I DSL
Sbjct: 151 FLLETNTICQQLQSNAFKNEIERLVNELKNTAQYTEDKLDILESKSDALIQTSSMIHDSL 210

Query: 196 NSAGIQIQQMAQTSRKLEDGMGDVLKRSEDVYEQSRRIETSQSELQEGQSKLRRSLEEGM 255
            S  +++Q +A  +  LE  +  + +++ ++ ++ + I  SQ  L++GQ K++ +L++GM
Sbjct: 211 GSLDVRVQNVASVTNTLETSVSGLSQQTVEISQEQKNIAESQLALRDGQVKMKETLKDGM 270

Query: 256 EMLQDSYKDLGREMDGLRDGAVEIEKEITKVGDAMFSKMTYLQSTADDIGNMAGLSLDKQ 315
           +M  D+Y ++   +D L+    +IE EI+ +G+ + +KM  LQST DDIG     SLDKQ
Sbjct: 271 DMFLDAYTNIQEGVDKLKSDTEQIEVEISVLGNNLSTKMIDLQSTTDDIGTKTRSSLDKQ 330

Query: 316 QELLDAQSIALNGLRSLSKVQSEALEESRSKLQQLAEYGHRQQEELLQRQKQLQQFHDRL 375
           Q+LLD Q++AL+G++ L++ QSEAL+ESR+ LQ+L E+   QQE+L +RQ++LQ+ HD L
Sbjct: 331 QKLLDGQTVALDGIQFLTRFQSEALQESRNTLQRLKEFSQEQQEDLAKRQEKLQEVHDHL 390

Query: 376 MENSQSIMAAQQSFESKQASIFIALDKLFTLHNAMLLESRLIKVFFIYFTSIFIIYMFSS 435
            ENS+S++ AQ +FE+KQA++F+ALDKLF LHNAMLLESR+IK F IYF SIF+IYMF+S
Sbjct: 391 FENSKSMLEAQVAFEAKQANMFVALDKLFALHNAMLLESRVIKAFVIYFLSIFVIYMFTS 450

Query: 436 TKQTYAVRPWLYIELCATFIFEVAILRLEVFDIEQQTWIINLVRIVFLFLAFFQLLHAIF 495
           TKQTY +RP LYI LC T   EVA LR  V D E+Q W+INL+R +F  LA  QLLHA  
Sbjct: 451 TKQTYIIRPRLYIGLCVTLALEVASLRY-VNDTERQAWMINLIRSLFALLASAQLLHAAL 510

Query: 496 TYRDYDALNYHMLLMLTERINEMQTPNKLSWGSD--SDLNWSSWIDTELSEDVEDL---D 555
           +YRDY+ LN+ +LL L +++N+MQ+  +LS+  D  S+++W+SW+DT+L++D ++L   D
Sbjct: 511 SYRDYEVLNHQILLRLVDKVNDMQSKKELSYDEDTESEVDWTSWVDTDLTDDDDNLADPD 570

Query: 556 YVLPEEIGENSITTTSTTRK-YNLRRR 571
           Y +P  I +N +TT+S TR+ YN R R
Sbjct: 571 YKIPLLIKDNPVTTSSLTRRLYNFRPR 593

BLAST of Tan0010176 vs. NCBI nr
Match: XP_022143299.1 (protein GAMETE EXPRESSED 1 [Momordica charantia] >XP_022143300.1 protein GAMETE EXPRESSED 1 [Momordica charantia])

HSP 1 Score: 897.9 bits (2319), Expect = 4.6e-257
Identity = 479/587 (81.60%), Postives = 520/587 (88.59%), Query Frame = 0

Query: 1   MEPVLFMVILL----------FVLSSSSSDSTTNPSKHY---SVAEFSMEGFDDRKGVKR 60
           ME  LF+VILL          +  SSSSSDS     K     +VAEFSMEGFDD+KGV+R
Sbjct: 1   MERFLFLVILLLASGKCESWIWFSSSSSSDSAMGSFKRIEGGTVAEFSMEGFDDQKGVRR 60

Query: 61  IENAKNKLTVSNSCWESAYRHLFVGCSEIFAVDEKRWRFAWHLTDCFQKDSGRPPFPSCD 120
           IENAKNKLT SNSCWESAYRHLF GCSEIFA DEKR RFAWHL+DCFQKDSGRPPFPSCD
Sbjct: 61  IENAKNKLTASNSCWESAYRHLFAGCSEIFAADEKRSRFAWHLSDCFQKDSGRPPFPSCD 120

Query: 121 PESAMAECLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHETERLVNELKRSSESAEAK 180
           P+SAM++CLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKH+TERLVNELKRSSE+ E K
Sbjct: 121 PKSAMSKCLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHDTERLVNELKRSSEAVEGK 180

Query: 181 LEIIEERSESLLQNSYHIFDSLNSAGIQIQQMAQTSRKLEDGMGDVLKRSEDVYEQSRRI 240
           LE IEE+SESLLQNSY IFDSLNS G+QIQQM QTSRKLED MG VLK SE VYEQ+++I
Sbjct: 181 LETIEEKSESLLQNSYQIFDSLNSTGVQIQQMVQTSRKLEDHMGVVLKHSEAVYEQTKKI 240

Query: 241 ETSQSELQEGQSKLRRSLEEGMEMLQDSYKDLGREMDGLRDGAVEIEKEITKVGDAMFSK 300
           ETSQSELQEGQ +LRRSLEEGMEMLQDSY +L REMDGLRD A+EIEKEITKVGDAMFSK
Sbjct: 241 ETSQSELQEGQLRLRRSLEEGMEMLQDSYSNLSREMDGLRDEAMEIEKEITKVGDAMFSK 300

Query: 301 MTYLQSTADDIGNMAGLSLDKQQELLDAQSIALNGLRSLSKVQSEALEESRSKLQQLAEY 360
           M YLQSTADDIGNMAGLSLDKQQELLDAQS ALNGL SLSKVQSEALEESRSKLQ LAEY
Sbjct: 301 MKYLQSTADDIGNMAGLSLDKQQELLDAQSTALNGLHSLSKVQSEALEESRSKLQDLAEY 360

Query: 361 GHRQQEELLQRQKQLQQFHDRLMENSQSIMAAQQSFESKQASIFIALDKLFTLHNAMLLE 420
           GHRQQEELLQRQ QLQQ HDRLMENS++I+ AQQSFESKQA++FIAL+KLFTLHNAMLLE
Sbjct: 361 GHRQQEELLQRQGQLQQLHDRLMENSKTILEAQQSFESKQANMFIALEKLFTLHNAMLLE 420

Query: 421 SRLIKVFFIYFTSIFIIYMFSSTKQTYAVRPWLYIELCATFIFEVAILRLEVFDIEQQTW 480
           SRLIKVFFIYFTS+FIIYMFSSTKQTY VRPWLYIELCA+F+ EVAILR+EVFD EQ+TW
Sbjct: 421 SRLIKVFFIYFTSVFIIYMFSSTKQTYTVRPWLYIELCASFLIEVAILRVEVFDTEQRTW 480

Query: 481 IINLVRIVFLFLAFFQLLHAIFTYRDYDALNYHMLLMLTERINEMQTPNKLSWGSDSDLN 540
           I+++VR  FL  A  QLLHAIFTYRDYD LN+HMLLML E+IN M+TPNKL W SDS++N
Sbjct: 481 IVSVVRTAFLLAASLQLLHAIFTYRDYDVLNHHMLLMLMEKINGMETPNKLCWESDSEVN 540

Query: 541 WSSWIDTELSED-VEDLDYVLPEEIGENSITTTSTTRKYNLRRRPLR 574
           WSSWIDTELSED VED D+VLPEE+GENSITT  TTR+YNLRRRPLR
Sbjct: 541 WSSWIDTELSEDNVEDPDFVLPEEVGENSITTAFTTRRYNLRRRPLR 587

BLAST of Tan0010176 vs. NCBI nr
Match: XP_038905677.1 (protein GAMETE EXPRESSED 1 [Benincasa hispida] >XP_038905679.1 protein GAMETE EXPRESSED 1 [Benincasa hispida])

HSP 1 Score: 872.8 bits (2254), Expect = 1.6e-249
Identity = 469/589 (79.63%), Postives = 517/589 (87.78%), Query Frame = 0

Query: 1   MEPVLFMVILLFVL---------SSSSSDSTTNPS-------KHYSVAEFSMEGFDDRKG 60
           ME +L +VILLF L         SSSSS S++  S       +  SV+EF++EGFDD+KG
Sbjct: 1   MEHILLLVILLFALPKSESWNWFSSSSSSSSSGSSVGSFNRIEGGSVSEFAIEGFDDQKG 60

Query: 61  VKRIENAKNKLTVSNSCWESAYRHLFVGCSEIFAVDEKRWRFAWHLTDCFQKDSGRPPFP 120
           V+RIENAKNKL+ SNSCWESAYRHLFVGCSEIFA DEKR RFAWHL+DCFQKDSGR  FP
Sbjct: 61  VRRIENAKNKLSSSNSCWESAYRHLFVGCSEIFAADEKRSRFAWHLSDCFQKDSGRVAFP 120

Query: 121 SCDPESAMAECLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHETERLVNELKRSSESA 180
           SCD +S MA+CLKYLNEHEHRIYLEFYLETNSICHQLQA+AFKH+TERLVNELKRSSE+A
Sbjct: 121 SCDAKSPMAKCLKYLNEHEHRIYLEFYLETNSICHQLQANAFKHDTERLVNELKRSSEAA 180

Query: 181 EAKLEIIEERSESLLQNSYHIFDSLNSAGIQIQQMAQTSRKLEDGMGDVLKRSEDVYEQS 240
           E KLE IEE+SESLLQ+SY I DSLNS GI+IQ+MAQTSRKLED MG VLK SE VYEQS
Sbjct: 181 EVKLESIEEKSESLLQSSYQISDSLNSTGIKIQKMAQTSRKLEDHMGIVLKHSEAVYEQS 240

Query: 241 RRIETSQSELQEGQSKLRRSLEEGMEMLQDSYKDLGREMDGLRDGAVEIEKEITKVGDAM 300
           ++IETSQ ELQEGQ KLR+SLEEGMEMLQDSY +L REMDGLR+ A+EIEKEITKVGD+M
Sbjct: 241 KKIETSQLELQEGQLKLRKSLEEGMEMLQDSYSNLSREMDGLREEAIEIEKEITKVGDSM 300

Query: 301 FSKMTYLQSTADDIGNMAGLSLDKQQELLDAQSIALNGLRSLSKVQSEALEESRSKLQQL 360
             KM YLQSTADDIGNMAGLSLDKQQELLDAQS ALNGL SLSKVQSEALEESRSKLQQL
Sbjct: 301 SLKMKYLQSTADDIGNMAGLSLDKQQELLDAQSTALNGLHSLSKVQSEALEESRSKLQQL 360

Query: 361 AEYGHRQQEELLQRQKQLQQFHDRLMENSQSIMAAQQSFESKQASIFIALDKLFTLHNAM 420
           AEYGH+QQEELLQRQ QLQQ HDRLM+NS+SI+ AQQ FESKQAS+FIAL+KLFTLHNAM
Sbjct: 361 AEYGHKQQEELLQRQGQLQQLHDRLMDNSKSILEAQQKFESKQASMFIALEKLFTLHNAM 420

Query: 421 LLESRLIKVFFIYFTSIFIIYMFSSTKQTYAVRPWLYIELCATFIFEVAILRLEVFDIEQ 480
           LLESRLIKVFFIYFTSIFIIYMFSSTKQTY VRPWLYIELC TF+ EVAILR E+FD+EQ
Sbjct: 421 LLESRLIKVFFIYFTSIFIIYMFSSTKQTYTVRPWLYIELCVTFLIEVAILRFEMFDMEQ 480

Query: 481 QTWIINLVRIVFLFLAFFQLLHAIFTYRDYDALNYHMLLMLTERINEMQTPNKLSWGSDS 540
           +TWI+N VR +FL +A  QLLHAI TYRDYD LN+HMLLML ERIN MQT NKLSW SDS
Sbjct: 481 KTWIVNSVRTMFLLIASLQLLHAICTYRDYDVLNHHMLLMLMERINGMQTQNKLSWDSDS 540

Query: 541 DLNWSSWIDTELSEDVEDLDYVLPEEIGENSITTTSTTRKYNLRRRPLR 574
           ++NWSSWIDTELSEDVED D+VLPEE+GENSITT ST+R+YNLR RPLR
Sbjct: 541 EVNWSSWIDTELSEDVEDPDFVLPEEVGENSITTASTSRRYNLRHRPLR 589

BLAST of Tan0010176 vs. NCBI nr
Match: XP_022983403.1 (protein GAMETE EXPRESSED 1 [Cucurbita maxima])

HSP 1 Score: 865.1 bits (2234), Expect = 3.3e-247
Identity = 460/586 (78.50%), Postives = 507/586 (86.52%), Query Frame = 0

Query: 1   MEPVLFMVILLFVL----------SSSSSDSTTNPSKHY---SVAEFSMEGFDDRKGVKR 60
           ME +LF++ILLF L          SSSSS S+    K     SV+EFS+EGFDD+KGV R
Sbjct: 130 MEHILFLLILLFALPNCESWNWFSSSSSSGSSMGSFKRVEGGSVSEFSIEGFDDQKGVSR 189

Query: 61  IENAKNKLTVSNSCWESAYRHLFVGCSEIFAVDEKRWRFAWHLTDCFQKDSGRPPFPSCD 120
           IENAKNKL+ SNSCWE AYRHLF GCSEIFA DEKR RFAWHL+DCFQKDSGRPPFPSCD
Sbjct: 190 IENAKNKLSTSNSCWEGAYRHLFAGCSEIFAADEKRSRFAWHLSDCFQKDSGRPPFPSCD 249

Query: 121 PESAMAECLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHETERLVNELKRSSESAEAK 180
            ESAMA+CLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKH+TERLVNELKRSSE+A+ K
Sbjct: 250 AESAMAKCLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHDTERLVNELKRSSEAAQGK 309

Query: 181 LEIIEERSESLLQNSYHIFDSLNSAGIQIQQMAQTSRKLEDGMGDVLKRSEDVYEQSRRI 240
           LE IEE+SESLLQNSY I+DSLNS G+QIQ+M QTSRKLED MG VL+ SE VYEQS++I
Sbjct: 310 LESIEEKSESLLQNSYQIYDSLNSTGVQIQKMVQTSRKLEDHMGTVLEHSEAVYEQSKKI 369

Query: 241 ETSQSELQEGQSKLRRSLEEGMEMLQDSYKDLGREMDGLRDGAVEIEKEITKVGDAMFSK 300
           ETSQ ELQEGQ KLRRSLEEGMEMLQDSY +LGREMDGLR+ A+EIEKEI KVGD+M  K
Sbjct: 370 ETSQLELQEGQLKLRRSLEEGMEMLQDSYSNLGREMDGLREEAIEIEKEIAKVGDSMSLK 429

Query: 301 MTYLQSTADDIGNMAGLSLDKQQELLDAQSIALNGLRSLSKVQSEALEESRSKLQQLAEY 360
           M YLQSTADDIGNMAGLSLDKQQELLDAQS ALNGL SLSKVQSEALE+SRSKLQQLAEY
Sbjct: 430 MKYLQSTADDIGNMAGLSLDKQQELLDAQSTALNGLHSLSKVQSEALEDSRSKLQQLAEY 489

Query: 361 GHRQQEELLQRQKQLQQFHDRLMENSQSIMAAQQSFESKQASIFIALDKLFTLHNAMLLE 420
           G +QQ+E LQRQ QLQQ HDRLM+NS SI+ AQQSFESKQA++FIAL+KLFTLHNAMLLE
Sbjct: 490 GRKQQDEFLQRQGQLQQLHDRLMDNSNSILEAQQSFESKQANMFIALEKLFTLHNAMLLE 549

Query: 421 SRLIKVFFIYFTSIFIIYMFSSTKQTYAVRPWLYIELCATFIFEVAILRLEVFDIEQQTW 480
           SRLIKVF IYF  IFI+YMFSSTKQTY VRPWLYIELC  F+ EV ILR E+F++EQ+TW
Sbjct: 550 SRLIKVFLIYFILIFIVYMFSSTKQTYTVRPWLYIELCVAFLIEVGILRFEMFNMEQKTW 609

Query: 481 IINLVRIVFLFLAFFQLLHAIFTYRDYDALNYHMLLMLTERINEMQTPNKLSWGSDSDLN 540
           IIN VR VFL  A  QLLHAI TYRDYD LN+HMLLML ERIN MQT NK+ W SDS++N
Sbjct: 610 IINSVRTVFLLAASLQLLHAICTYRDYDVLNHHMLLMLMERINGMQTQNKICWESDSEVN 669

Query: 541 WSSWIDTELSEDVEDLDYVLPEEIGENSITTTSTTRKYNLRRRPLR 574
           WSSWIDTELSEDV+D D+VLPEE+GENSIT+ STTR+YNLR RPLR
Sbjct: 670 WSSWIDTELSEDVDDPDFVLPEEVGENSITSASTTRRYNLRHRPLR 715

BLAST of Tan0010176 vs. NCBI nr
Match: XP_023529107.1 (protein GAMETE EXPRESSED 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 862.1 bits (2226), Expect = 2.8e-246
Identity = 459/586 (78.33%), Postives = 505/586 (86.18%), Query Frame = 0

Query: 1   MEPVLFMVILLFVL----------SSSSSDSTTNPSKHY---SVAEFSMEGFDDRKGVKR 60
           ME +LF+VILLF L          SSSSS S+    K     SV+EFS+EGFDD+KGV+R
Sbjct: 79  MEHILFLVILLFALPNCESWNWFSSSSSSGSSMGSFKSVEGGSVSEFSIEGFDDQKGVRR 138

Query: 61  IENAKNKLTVSNSCWESAYRHLFVGCSEIFAVDEKRWRFAWHLTDCFQKDSGRPPFPSCD 120
           IENAKNKL+ SNSCWE AYRHLF GCSEIFA DEKR RFAWHL+DCFQKDSGRPPFPSCD
Sbjct: 139 IENAKNKLSTSNSCWEGAYRHLFAGCSEIFAADEKRSRFAWHLSDCFQKDSGRPPFPSCD 198

Query: 121 PESAMAECLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHETERLVNELKRSSESAEAK 180
            ES MA+CLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKH+TERLVNELKRSSE+AE K
Sbjct: 199 AESPMAKCLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHDTERLVNELKRSSEAAEGK 258

Query: 181 LEIIEERSESLLQNSYHIFDSLNSAGIQIQQMAQTSRKLEDGMGDVLKRSEDVYEQSRRI 240
           LE IEE+SESLLQNSY I+DSLNS G+QIQ+M QTSRKLED MG VL+ SE VYEQS++I
Sbjct: 259 LESIEEKSESLLQNSYQIYDSLNSTGVQIQKMVQTSRKLEDHMGTVLEHSEAVYEQSKKI 318

Query: 241 ETSQSELQEGQSKLRRSLEEGMEMLQDSYKDLGREMDGLRDGAVEIEKEITKVGDAMFSK 300
           ETSQ ELQEGQ KLRRSLEEGMEMLQDSY +LGREMDGLR+ A+EIEKEI KVGD+M  K
Sbjct: 319 ETSQLELQEGQLKLRRSLEEGMEMLQDSYSNLGREMDGLREEAIEIEKEIAKVGDSMSLK 378

Query: 301 MTYLQSTADDIGNMAGLSLDKQQELLDAQSIALNGLRSLSKVQSEALEESRSKLQQLAEY 360
           M YLQSTADDIGNMAGLSLDKQQELLDAQS ALNGL SLSKVQSEALE+SRSKLQQLAEY
Sbjct: 379 MKYLQSTADDIGNMAGLSLDKQQELLDAQSTALNGLHSLSKVQSEALEDSRSKLQQLAEY 438

Query: 361 GHRQQEELLQRQKQLQQFHDRLMENSQSIMAAQQSFESKQASIFIALDKLFTLHNAMLLE 420
           G +QQ+E LQRQ QLQQ HDRLM+NS SI+ AQQSFESKQA++FIAL+KLFTLHNAMLLE
Sbjct: 439 GRKQQDEFLQRQGQLQQLHDRLMDNSNSILEAQQSFESKQANMFIALEKLFTLHNAMLLE 498

Query: 421 SRLIKVFFIYFTSIFIIYMFSSTKQTYAVRPWLYIELCATFIFEVAILRLEVFDIEQQTW 480
           SRLIKVF IYF  IFI+YMFSSTKQTY VRPWLYIELC  F+ EV ILR E+F++EQ+TW
Sbjct: 499 SRLIKVFLIYFILIFIVYMFSSTKQTYTVRPWLYIELCVAFLIEVGILRFEMFNMEQKTW 558

Query: 481 IINLVRIVFLFLAFFQLLHAIFTYRDYDALNYHMLLMLTERINEMQTPNKLSWGSDSDLN 540
           IIN VR VFL  A  QLLHAI TYRDYD LN+HMLLML ERIN MQT NK+ W SDS++N
Sbjct: 559 IINSVRTVFLLAASLQLLHAICTYRDYDVLNHHMLLMLMERINGMQTQNKICWESDSEVN 618

Query: 541 WSSWIDTELSEDVEDLDYVLPEEIGENSITTTSTTRKYNLRRRPLR 574
           WSSWIDTELSED +D D+VLPEE+GENSIT+  TTR+YNLR RPLR
Sbjct: 619 WSSWIDTELSEDADDPDFVLPEEVGENSITSALTTRRYNLRHRPLR 664

BLAST of Tan0010176 vs. NCBI nr
Match: XP_022935308.1 (protein GAMETE EXPRESSED 1 [Cucurbita moschata])

HSP 1 Score: 862.1 bits (2226), Expect = 2.8e-246
Identity = 459/586 (78.33%), Postives = 505/586 (86.18%), Query Frame = 0

Query: 1   MEPVLFMVILLFVL----------SSSSSDSTTNPSKHY---SVAEFSMEGFDDRKGVKR 60
           ME +LF+VILLF L          SSSSS S+    K     SV+EFS+EGFDD+KGV+R
Sbjct: 1   MEHILFLVILLFALPNCESWNWFSSSSSSGSSMGSFKRVEGGSVSEFSIEGFDDQKGVRR 60

Query: 61  IENAKNKLTVSNSCWESAYRHLFVGCSEIFAVDEKRWRFAWHLTDCFQKDSGRPPFPSCD 120
           IENAKNKL+ SNSCWE AYRHLF GCSEIFA DEKR RFAWHL+DCFQKDSGRPPFPSCD
Sbjct: 61  IENAKNKLSTSNSCWEGAYRHLFAGCSEIFAADEKRSRFAWHLSDCFQKDSGRPPFPSCD 120

Query: 121 PESAMAECLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHETERLVNELKRSSESAEAK 180
            ES MA+CLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKH+TERLVNELKRSSE+AE K
Sbjct: 121 AESPMAKCLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHDTERLVNELKRSSEAAEGK 180

Query: 181 LEIIEERSESLLQNSYHIFDSLNSAGIQIQQMAQTSRKLEDGMGDVLKRSEDVYEQSRRI 240
           LE IEE+SESLLQNSY I+DSLNS G+QIQ+M QTSRKLED MG VL+ SE VYEQS++I
Sbjct: 181 LESIEEKSESLLQNSYQIYDSLNSTGVQIQKMVQTSRKLEDHMGTVLEHSEAVYEQSKKI 240

Query: 241 ETSQSELQEGQSKLRRSLEEGMEMLQDSYKDLGREMDGLRDGAVEIEKEITKVGDAMFSK 300
           ETSQ ELQEGQ KLRRSLEEGMEMLQDSY +LGREMDGLR+ A+EIEKEI KVGD+M  K
Sbjct: 241 ETSQLELQEGQLKLRRSLEEGMEMLQDSYSNLGREMDGLREEAIEIEKEIAKVGDSMSLK 300

Query: 301 MTYLQSTADDIGNMAGLSLDKQQELLDAQSIALNGLRSLSKVQSEALEESRSKLQQLAEY 360
           M YLQSTADDIGNMAGLSLDKQQELLDAQS ALNGL SLSKVQSEALE+SR KLQQLAEY
Sbjct: 301 MKYLQSTADDIGNMAGLSLDKQQELLDAQSTALNGLHSLSKVQSEALEDSRRKLQQLAEY 360

Query: 361 GHRQQEELLQRQKQLQQFHDRLMENSQSIMAAQQSFESKQASIFIALDKLFTLHNAMLLE 420
           G +QQ+E LQRQ QLQQ HDRLM+NS SI+ AQQSFESKQA++FIAL+KLFTLHNAMLLE
Sbjct: 361 GRKQQDEFLQRQGQLQQLHDRLMDNSNSILEAQQSFESKQANMFIALEKLFTLHNAMLLE 420

Query: 421 SRLIKVFFIYFTSIFIIYMFSSTKQTYAVRPWLYIELCATFIFEVAILRLEVFDIEQQTW 480
           SRLIKVF IYF  IFI+YMFSSTKQTY VRPWLYIELC  F+ EV ILR E+F++EQ+TW
Sbjct: 421 SRLIKVFLIYFILIFIVYMFSSTKQTYTVRPWLYIELCVAFLIEVGILRFEMFNMEQKTW 480

Query: 481 IINLVRIVFLFLAFFQLLHAIFTYRDYDALNYHMLLMLTERINEMQTPNKLSWGSDSDLN 540
           IIN VR VFL  A  QLLHAI TYRDYD LN+HMLLML ERIN MQT NK+ W SDS++N
Sbjct: 481 IINSVRTVFLLAASLQLLHAICTYRDYDVLNHHMLLMLMERINGMQTQNKICWESDSEVN 540

Query: 541 WSSWIDTELSEDVEDLDYVLPEEIGENSITTTSTTRKYNLRRRPLR 574
           WSSWIDTELSEDV+D D+VLPEE+GENSIT+  TTR+YNLR RPLR
Sbjct: 541 WSSWIDTELSEDVDDPDFVLPEEVGENSITSALTTRRYNLRHRPLR 586

BLAST of Tan0010176 vs. ExPASy TrEMBL
Match: A0A6J1CNX6 (protein GAMETE EXPRESSED 1 OS=Momordica charantia OX=3673 GN=LOC111013205 PE=4 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 2.2e-257
Identity = 479/587 (81.60%), Postives = 520/587 (88.59%), Query Frame = 0

Query: 1   MEPVLFMVILL----------FVLSSSSSDSTTNPSKHY---SVAEFSMEGFDDRKGVKR 60
           ME  LF+VILL          +  SSSSSDS     K     +VAEFSMEGFDD+KGV+R
Sbjct: 1   MERFLFLVILLLASGKCESWIWFSSSSSSDSAMGSFKRIEGGTVAEFSMEGFDDQKGVRR 60

Query: 61  IENAKNKLTVSNSCWESAYRHLFVGCSEIFAVDEKRWRFAWHLTDCFQKDSGRPPFPSCD 120
           IENAKNKLT SNSCWESAYRHLF GCSEIFA DEKR RFAWHL+DCFQKDSGRPPFPSCD
Sbjct: 61  IENAKNKLTASNSCWESAYRHLFAGCSEIFAADEKRSRFAWHLSDCFQKDSGRPPFPSCD 120

Query: 121 PESAMAECLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHETERLVNELKRSSESAEAK 180
           P+SAM++CLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKH+TERLVNELKRSSE+ E K
Sbjct: 121 PKSAMSKCLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHDTERLVNELKRSSEAVEGK 180

Query: 181 LEIIEERSESLLQNSYHIFDSLNSAGIQIQQMAQTSRKLEDGMGDVLKRSEDVYEQSRRI 240
           LE IEE+SESLLQNSY IFDSLNS G+QIQQM QTSRKLED MG VLK SE VYEQ+++I
Sbjct: 181 LETIEEKSESLLQNSYQIFDSLNSTGVQIQQMVQTSRKLEDHMGVVLKHSEAVYEQTKKI 240

Query: 241 ETSQSELQEGQSKLRRSLEEGMEMLQDSYKDLGREMDGLRDGAVEIEKEITKVGDAMFSK 300
           ETSQSELQEGQ +LRRSLEEGMEMLQDSY +L REMDGLRD A+EIEKEITKVGDAMFSK
Sbjct: 241 ETSQSELQEGQLRLRRSLEEGMEMLQDSYSNLSREMDGLRDEAMEIEKEITKVGDAMFSK 300

Query: 301 MTYLQSTADDIGNMAGLSLDKQQELLDAQSIALNGLRSLSKVQSEALEESRSKLQQLAEY 360
           M YLQSTADDIGNMAGLSLDKQQELLDAQS ALNGL SLSKVQSEALEESRSKLQ LAEY
Sbjct: 301 MKYLQSTADDIGNMAGLSLDKQQELLDAQSTALNGLHSLSKVQSEALEESRSKLQDLAEY 360

Query: 361 GHRQQEELLQRQKQLQQFHDRLMENSQSIMAAQQSFESKQASIFIALDKLFTLHNAMLLE 420
           GHRQQEELLQRQ QLQQ HDRLMENS++I+ AQQSFESKQA++FIAL+KLFTLHNAMLLE
Sbjct: 361 GHRQQEELLQRQGQLQQLHDRLMENSKTILEAQQSFESKQANMFIALEKLFTLHNAMLLE 420

Query: 421 SRLIKVFFIYFTSIFIIYMFSSTKQTYAVRPWLYIELCATFIFEVAILRLEVFDIEQQTW 480
           SRLIKVFFIYFTS+FIIYMFSSTKQTY VRPWLYIELCA+F+ EVAILR+EVFD EQ+TW
Sbjct: 421 SRLIKVFFIYFTSVFIIYMFSSTKQTYTVRPWLYIELCASFLIEVAILRVEVFDTEQRTW 480

Query: 481 IINLVRIVFLFLAFFQLLHAIFTYRDYDALNYHMLLMLTERINEMQTPNKLSWGSDSDLN 540
           I+++VR  FL  A  QLLHAIFTYRDYD LN+HMLLML E+IN M+TPNKL W SDS++N
Sbjct: 481 IVSVVRTAFLLAASLQLLHAIFTYRDYDVLNHHMLLMLMEKINGMETPNKLCWESDSEVN 540

Query: 541 WSSWIDTELSED-VEDLDYVLPEEIGENSITTTSTTRKYNLRRRPLR 574
           WSSWIDTELSED VED D+VLPEE+GENSITT  TTR+YNLRRRPLR
Sbjct: 541 WSSWIDTELSEDNVEDPDFVLPEEVGENSITTAFTTRRYNLRRRPLR 587

BLAST of Tan0010176 vs. ExPASy TrEMBL
Match: A0A6J1J7N6 (protein GAMETE EXPRESSED 1 OS=Cucurbita maxima OX=3661 GN=LOC111482011 PE=4 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.6e-247
Identity = 460/586 (78.50%), Postives = 507/586 (86.52%), Query Frame = 0

Query: 1   MEPVLFMVILLFVL----------SSSSSDSTTNPSKHY---SVAEFSMEGFDDRKGVKR 60
           ME +LF++ILLF L          SSSSS S+    K     SV+EFS+EGFDD+KGV R
Sbjct: 130 MEHILFLLILLFALPNCESWNWFSSSSSSGSSMGSFKRVEGGSVSEFSIEGFDDQKGVSR 189

Query: 61  IENAKNKLTVSNSCWESAYRHLFVGCSEIFAVDEKRWRFAWHLTDCFQKDSGRPPFPSCD 120
           IENAKNKL+ SNSCWE AYRHLF GCSEIFA DEKR RFAWHL+DCFQKDSGRPPFPSCD
Sbjct: 190 IENAKNKLSTSNSCWEGAYRHLFAGCSEIFAADEKRSRFAWHLSDCFQKDSGRPPFPSCD 249

Query: 121 PESAMAECLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHETERLVNELKRSSESAEAK 180
            ESAMA+CLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKH+TERLVNELKRSSE+A+ K
Sbjct: 250 AESAMAKCLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHDTERLVNELKRSSEAAQGK 309

Query: 181 LEIIEERSESLLQNSYHIFDSLNSAGIQIQQMAQTSRKLEDGMGDVLKRSEDVYEQSRRI 240
           LE IEE+SESLLQNSY I+DSLNS G+QIQ+M QTSRKLED MG VL+ SE VYEQS++I
Sbjct: 310 LESIEEKSESLLQNSYQIYDSLNSTGVQIQKMVQTSRKLEDHMGTVLEHSEAVYEQSKKI 369

Query: 241 ETSQSELQEGQSKLRRSLEEGMEMLQDSYKDLGREMDGLRDGAVEIEKEITKVGDAMFSK 300
           ETSQ ELQEGQ KLRRSLEEGMEMLQDSY +LGREMDGLR+ A+EIEKEI KVGD+M  K
Sbjct: 370 ETSQLELQEGQLKLRRSLEEGMEMLQDSYSNLGREMDGLREEAIEIEKEIAKVGDSMSLK 429

Query: 301 MTYLQSTADDIGNMAGLSLDKQQELLDAQSIALNGLRSLSKVQSEALEESRSKLQQLAEY 360
           M YLQSTADDIGNMAGLSLDKQQELLDAQS ALNGL SLSKVQSEALE+SRSKLQQLAEY
Sbjct: 430 MKYLQSTADDIGNMAGLSLDKQQELLDAQSTALNGLHSLSKVQSEALEDSRSKLQQLAEY 489

Query: 361 GHRQQEELLQRQKQLQQFHDRLMENSQSIMAAQQSFESKQASIFIALDKLFTLHNAMLLE 420
           G +QQ+E LQRQ QLQQ HDRLM+NS SI+ AQQSFESKQA++FIAL+KLFTLHNAMLLE
Sbjct: 490 GRKQQDEFLQRQGQLQQLHDRLMDNSNSILEAQQSFESKQANMFIALEKLFTLHNAMLLE 549

Query: 421 SRLIKVFFIYFTSIFIIYMFSSTKQTYAVRPWLYIELCATFIFEVAILRLEVFDIEQQTW 480
           SRLIKVF IYF  IFI+YMFSSTKQTY VRPWLYIELC  F+ EV ILR E+F++EQ+TW
Sbjct: 550 SRLIKVFLIYFILIFIVYMFSSTKQTYTVRPWLYIELCVAFLIEVGILRFEMFNMEQKTW 609

Query: 481 IINLVRIVFLFLAFFQLLHAIFTYRDYDALNYHMLLMLTERINEMQTPNKLSWGSDSDLN 540
           IIN VR VFL  A  QLLHAI TYRDYD LN+HMLLML ERIN MQT NK+ W SDS++N
Sbjct: 610 IINSVRTVFLLAASLQLLHAICTYRDYDVLNHHMLLMLMERINGMQTQNKICWESDSEVN 669

Query: 541 WSSWIDTELSEDVEDLDYVLPEEIGENSITTTSTTRKYNLRRRPLR 574
           WSSWIDTELSEDV+D D+VLPEE+GENSIT+ STTR+YNLR RPLR
Sbjct: 670 WSSWIDTELSEDVDDPDFVLPEEVGENSITSASTTRRYNLRHRPLR 715

BLAST of Tan0010176 vs. ExPASy TrEMBL
Match: A0A6J1FA68 (protein GAMETE EXPRESSED 1 OS=Cucurbita moschata OX=3662 GN=LOC111442231 PE=4 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 1.3e-246
Identity = 459/586 (78.33%), Postives = 505/586 (86.18%), Query Frame = 0

Query: 1   MEPVLFMVILLFVL----------SSSSSDSTTNPSKHY---SVAEFSMEGFDDRKGVKR 60
           ME +LF+VILLF L          SSSSS S+    K     SV+EFS+EGFDD+KGV+R
Sbjct: 1   MEHILFLVILLFALPNCESWNWFSSSSSSGSSMGSFKRVEGGSVSEFSIEGFDDQKGVRR 60

Query: 61  IENAKNKLTVSNSCWESAYRHLFVGCSEIFAVDEKRWRFAWHLTDCFQKDSGRPPFPSCD 120
           IENAKNKL+ SNSCWE AYRHLF GCSEIFA DEKR RFAWHL+DCFQKDSGRPPFPSCD
Sbjct: 61  IENAKNKLSTSNSCWEGAYRHLFAGCSEIFAADEKRSRFAWHLSDCFQKDSGRPPFPSCD 120

Query: 121 PESAMAECLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHETERLVNELKRSSESAEAK 180
            ES MA+CLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKH+TERLVNELKRSSE+AE K
Sbjct: 121 AESPMAKCLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHDTERLVNELKRSSEAAEGK 180

Query: 181 LEIIEERSESLLQNSYHIFDSLNSAGIQIQQMAQTSRKLEDGMGDVLKRSEDVYEQSRRI 240
           LE IEE+SESLLQNSY I+DSLNS G+QIQ+M QTSRKLED MG VL+ SE VYEQS++I
Sbjct: 181 LESIEEKSESLLQNSYQIYDSLNSTGVQIQKMVQTSRKLEDHMGTVLEHSEAVYEQSKKI 240

Query: 241 ETSQSELQEGQSKLRRSLEEGMEMLQDSYKDLGREMDGLRDGAVEIEKEITKVGDAMFSK 300
           ETSQ ELQEGQ KLRRSLEEGMEMLQDSY +LGREMDGLR+ A+EIEKEI KVGD+M  K
Sbjct: 241 ETSQLELQEGQLKLRRSLEEGMEMLQDSYSNLGREMDGLREEAIEIEKEIAKVGDSMSLK 300

Query: 301 MTYLQSTADDIGNMAGLSLDKQQELLDAQSIALNGLRSLSKVQSEALEESRSKLQQLAEY 360
           M YLQSTADDIGNMAGLSLDKQQELLDAQS ALNGL SLSKVQSEALE+SR KLQQLAEY
Sbjct: 301 MKYLQSTADDIGNMAGLSLDKQQELLDAQSTALNGLHSLSKVQSEALEDSRRKLQQLAEY 360

Query: 361 GHRQQEELLQRQKQLQQFHDRLMENSQSIMAAQQSFESKQASIFIALDKLFTLHNAMLLE 420
           G +QQ+E LQRQ QLQQ HDRLM+NS SI+ AQQSFESKQA++FIAL+KLFTLHNAMLLE
Sbjct: 361 GRKQQDEFLQRQGQLQQLHDRLMDNSNSILEAQQSFESKQANMFIALEKLFTLHNAMLLE 420

Query: 421 SRLIKVFFIYFTSIFIIYMFSSTKQTYAVRPWLYIELCATFIFEVAILRLEVFDIEQQTW 480
           SRLIKVF IYF  IFI+YMFSSTKQTY VRPWLYIELC  F+ EV ILR E+F++EQ+TW
Sbjct: 421 SRLIKVFLIYFILIFIVYMFSSTKQTYTVRPWLYIELCVAFLIEVGILRFEMFNMEQKTW 480

Query: 481 IINLVRIVFLFLAFFQLLHAIFTYRDYDALNYHMLLMLTERINEMQTPNKLSWGSDSDLN 540
           IIN VR VFL  A  QLLHAI TYRDYD LN+HMLLML ERIN MQT NK+ W SDS++N
Sbjct: 481 IINSVRTVFLLAASLQLLHAICTYRDYDVLNHHMLLMLMERINGMQTQNKICWESDSEVN 540

Query: 541 WSSWIDTELSEDVEDLDYVLPEEIGENSITTTSTTRKYNLRRRPLR 574
           WSSWIDTELSEDV+D D+VLPEE+GENSIT+  TTR+YNLR RPLR
Sbjct: 541 WSSWIDTELSEDVDDPDFVLPEEVGENSITSALTTRRYNLRHRPLR 586

BLAST of Tan0010176 vs. ExPASy TrEMBL
Match: A0A5A7TSK8 (Protein GAMETE EXPRESSED 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00920 PE=4 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 2.1e-244
Identity = 452/582 (77.66%), Postives = 511/582 (87.80%), Query Frame = 0

Query: 1   MEPVLFMVILLFVL----------SSSSSDSTTNPSKHYSVAEFSMEGFDDRKGVKRIEN 60
           M+ +LF+VILLF L          SSSSS S+ +  +  SVAEF++EGFDD+KGV+RIEN
Sbjct: 1   MDRILFLVILLFALPNCESWNWFSSSSSSGSSFDRVEGGSVAEFAIEGFDDQKGVRRIEN 60

Query: 61  AKNKLTVSNSCWESAYRHLFVGCSEIFAVDEKRWRFAWHLTDCFQKDSGRPPFPSCDPES 120
           AKNKL+VSNSCW SAYRHLF GCSEIFA DEKR RFAWHL+DCFQKDSGRP FP+CD +S
Sbjct: 61  AKNKLSVSNSCWGSAYRHLFAGCSEIFAADEKRSRFAWHLSDCFQKDSGRPSFPNCDAKS 120

Query: 121 AMAECLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHETERLVNELKRSSESAEAKLEI 180
            MA+CLKYLNEHEHRIYLEFYLETNSICHQLQA+AFKH+TERLVNELKRSSE+AE KLE 
Sbjct: 121 PMAKCLKYLNEHEHRIYLEFYLETNSICHQLQANAFKHDTERLVNELKRSSEAAEGKLES 180

Query: 181 IEERSESLLQNSYHIFDSLNSAGIQIQQMAQTSRKLEDGMGDVLKRSEDVYEQSRRIETS 240
           IEE+SESLLQNSY I DSL S G QIQ+MAQTSRKLED MG VLK SE VYEQS++IETS
Sbjct: 181 IEEKSESLLQNSYEISDSLKSTGTQIQKMAQTSRKLEDHMGVVLKHSEAVYEQSKKIETS 240

Query: 241 QSELQEGQSKLRRSLEEGMEMLQDSYKDLGREMDGLRDGAVEIEKEITKVGDAMFSKMTY 300
           Q ELQEGQ KLR+SLEEGMEMLQDSY +LG+EMD LR  A+EIEKEITKVGD+M  KM Y
Sbjct: 241 QLELQEGQLKLRKSLEEGMEMLQDSYSNLGQEMDSLRGEAIEIEKEITKVGDSMSLKMKY 300

Query: 301 LQSTADDIGNMAGLSLDKQQELLDAQSIALNGLRSLSKVQSEALEESRSKLQQLAEYGHR 360
           LQSTADDIGNMAGLSLDKQQELLDAQS ALNGL SLSKVQSEALEESR+KLQQLAEYGH+
Sbjct: 301 LQSTADDIGNMAGLSLDKQQELLDAQSTALNGLHSLSKVQSEALEESRNKLQQLAEYGHK 360

Query: 361 QQEELLQRQKQLQQFHDRLMENSQSIMAAQQSFESKQASIFIALDKLFTLHNAMLLESRL 420
           QQEELLQRQ QLQQ HDRLM+NS+SI+ AQQ FESKQAS+F+AL+KLFTLHNAMLLESRL
Sbjct: 361 QQEELLQRQGQLQQLHDRLMDNSKSILEAQQIFESKQASMFVALEKLFTLHNAMLLESRL 420

Query: 421 IKVFFIYFTSIFIIYMFSSTKQTYAVRPWLYIELCATFIFEVAILRLEVFDIEQQTWIIN 480
           IKVFFIYFTSIFIIYMFSSTKQTY VRPWLYIELC TF  EVAILR E+F++E++TWI+N
Sbjct: 421 IKVFFIYFTSIFIIYMFSSTKQTYTVRPWLYIELCVTFFIEVAILRFEMFNMEEKTWIVN 480

Query: 481 LVRIVFLFLAFFQLLHAIFTYRDYDALNYHMLLMLTERINEMQTPNKLSWGSDSDLNWSS 540
            +R +FL +A  QLL+A+ TYRDYD LN+HMLL+L ER+N MQT NKLSW SDS+++W+S
Sbjct: 481 SLRTMFLLIASLQLLYAVCTYRDYDVLNHHMLLLLMERVNGMQTQNKLSWDSDSEVDWTS 540

Query: 541 WIDTELSEDVEDLDYVLPEEIGENSITTTSTTRKYNLRRRPL 573
           WIDT+L EDVED D+VLPEE+GENSITT ST+R+YNLR RP+
Sbjct: 541 WIDTDLPEDVEDPDFVLPEEVGENSITTASTSRRYNLRHRPI 582

BLAST of Tan0010176 vs. ExPASy TrEMBL
Match: A0A1S3BZN2 (protein GAMETE EXPRESSED 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494785 PE=4 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 2.1e-244
Identity = 452/582 (77.66%), Postives = 511/582 (87.80%), Query Frame = 0

Query: 1   MEPVLFMVILLFVL----------SSSSSDSTTNPSKHYSVAEFSMEGFDDRKGVKRIEN 60
           M+ +LF+VILLF L          SSSSS S+ +  +  SVAEF++EGFDD+KGV+RIEN
Sbjct: 1   MDRILFLVILLFALPNCESWNWFSSSSSSGSSFDRVEGGSVAEFAIEGFDDQKGVRRIEN 60

Query: 61  AKNKLTVSNSCWESAYRHLFVGCSEIFAVDEKRWRFAWHLTDCFQKDSGRPPFPSCDPES 120
           AKNKL+VSNSCW SAYRHLF GCSEIFA DEKR RFAWHL+DCFQKDSGRP FP+CD +S
Sbjct: 61  AKNKLSVSNSCWGSAYRHLFAGCSEIFAADEKRSRFAWHLSDCFQKDSGRPSFPNCDAKS 120

Query: 121 AMAECLKYLNEHEHRIYLEFYLETNSICHQLQAHAFKHETERLVNELKRSSESAEAKLEI 180
            MA+CLKYLNEHEHRIYLEFYLETNSICHQLQA+AFKH+TERLVNELKRSSE+AE KLE 
Sbjct: 121 PMAKCLKYLNEHEHRIYLEFYLETNSICHQLQANAFKHDTERLVNELKRSSEAAEGKLES 180

Query: 181 IEERSESLLQNSYHIFDSLNSAGIQIQQMAQTSRKLEDGMGDVLKRSEDVYEQSRRIETS 240
           IEE+SESLLQNSY I DSL S G QIQ+MAQTSRKLED MG VLK SE VYEQS++IETS
Sbjct: 181 IEEKSESLLQNSYEISDSLKSTGTQIQKMAQTSRKLEDHMGVVLKHSEAVYEQSKKIETS 240

Query: 241 QSELQEGQSKLRRSLEEGMEMLQDSYKDLGREMDGLRDGAVEIEKEITKVGDAMFSKMTY 300
           Q ELQEGQ KLR+SLEEGMEMLQDSY +LG+EMD LR  A+EIEKEITKVGD+M  KM Y
Sbjct: 241 QLELQEGQLKLRKSLEEGMEMLQDSYSNLGQEMDSLRGEAIEIEKEITKVGDSMSLKMKY 300

Query: 301 LQSTADDIGNMAGLSLDKQQELLDAQSIALNGLRSLSKVQSEALEESRSKLQQLAEYGHR 360
           LQSTADDIGNMAGLSLDKQQELLDAQS ALNGL SLSKVQSEALEESR+KLQQLAEYGH+
Sbjct: 301 LQSTADDIGNMAGLSLDKQQELLDAQSTALNGLHSLSKVQSEALEESRNKLQQLAEYGHK 360

Query: 361 QQEELLQRQKQLQQFHDRLMENSQSIMAAQQSFESKQASIFIALDKLFTLHNAMLLESRL 420
           QQEELLQRQ QLQQ HDRLM+NS+SI+ AQQ FESKQAS+F+AL+KLFTLHNAMLLESRL
Sbjct: 361 QQEELLQRQGQLQQLHDRLMDNSKSILEAQQIFESKQASMFVALEKLFTLHNAMLLESRL 420

Query: 421 IKVFFIYFTSIFIIYMFSSTKQTYAVRPWLYIELCATFIFEVAILRLEVFDIEQQTWIIN 480
           IKVFFIYFTSIFIIYMFSSTKQTY VRPWLYIELC TF  EVAILR E+F++E++TWI+N
Sbjct: 421 IKVFFIYFTSIFIIYMFSSTKQTYTVRPWLYIELCVTFFIEVAILRFEMFNMEEKTWIVN 480

Query: 481 LVRIVFLFLAFFQLLHAIFTYRDYDALNYHMLLMLTERINEMQTPNKLSWGSDSDLNWSS 540
            +R +FL +A  QLL+A+ TYRDYD LN+HMLL+L ER+N MQT NKLSW SDS+++W+S
Sbjct: 481 SLRTMFLLIASLQLLYAVCTYRDYDVLNHHMLLLLMERVNGMQTQNKLSWDSDSEVDWTS 540

Query: 541 WIDTELSEDVEDLDYVLPEEIGENSITTTSTTRKYNLRRRPL 573
           WIDT+L EDVED D+VLPEE+GENSITT ST+R+YNLR RP+
Sbjct: 541 WIDTDLPEDVEDPDFVLPEEVGENSITTASTSRRYNLRHRPI 582

BLAST of Tan0010176 vs. TAIR 10
Match: AT5G55490.1 (gamete expressed protein 1 )

HSP 1 Score: 515.8 bits (1327), Expect = 4.6e-146
Identity = 284/567 (50.09%), Postives = 406/567 (71.60%), Query Frame = 0

Query: 16  SSSSDSTTNP------SKHYSVAEFSMEGFDDRKGVKRIENAKNKLTVSNSCWESAYRHL 75
           SSSS S  +P          S  +FSME F D+K V+ +E   NKL    SCW++AY +L
Sbjct: 31  SSSSSSAEDPYSSSFSRSRKSNPDFSMEVFSDQKAVQVLE---NKLVGLTSCWQNAYSYL 90

Query: 76  FVGCSEIFAVDEKRWRFAWHLTDCFQKDSGRPPFPSCDPESAMAECLKYLNEHEHRIYLE 135
             GC E  A +EKR RFAW+L+DCF KDSGRP FP+C  ES M  CLK L++HEH+IYL+
Sbjct: 91  LAGCKETIATEEKRKRFAWYLSDCFIKDSGRPAFPTCKDESVMMSCLKKLDDHEHKIYLD 150

Query: 136 FYLETNSICHQLQAHAFKHETERLVNELKRSSESAEAKLEIIEERSESLLQNSYHIFDSL 195
           F LETN+IC QLQ++AFK+E ERLVNELK +++  E KL+I+E +S++L+Q S  I DSL
Sbjct: 151 FLLETNTICQQLQSNAFKNEIERLVNELKNTAQYTEDKLDILESKSDALIQTSSMIHDSL 210

Query: 196 NSAGIQIQQMAQTSRKLEDGMGDVLKRSEDVYEQSRRIETSQSELQEGQSKLRRSLEEGM 255
            S  +++Q +A  +  LE  +  + +++ ++ ++ + I  SQ  L++GQ K++ +L++GM
Sbjct: 211 GSLDVRVQNVASVTNTLETSVSGLSQQTVEISQEQKNIAESQLALRDGQVKMKETLKDGM 270

Query: 256 EMLQDSYKDLGREMDGLRDGAVEIEKEITKVGDAMFSKMTYLQSTADDIGNMAGLSLDKQ 315
           +M  D+Y ++   +D L+    +IE EI+ +G+ + +KM  LQST DDIG     SLDKQ
Sbjct: 271 DMFLDAYTNIQEGVDKLKSDTEQIEVEISVLGNNLSTKMIDLQSTTDDIGTKTRSSLDKQ 330

Query: 316 QELLDAQSIALNGLRSLSKVQSEALEESRSKLQQLAEYGHRQQEELLQRQKQLQQFHDRL 375
           Q+LLD Q++AL+G++ L++ QSEAL+ESR+ LQ+L E+   QQE+L +RQ++LQ+ HD L
Sbjct: 331 QKLLDGQTVALDGIQFLTRFQSEALQESRNTLQRLKEFSQEQQEDLAKRQEKLQEVHDHL 390

Query: 376 MENSQSIMAAQQSFESKQASIFIALDKLFTLHNAMLLESRLIKVFFIYFTSIFIIYMFSS 435
            ENS+S++ AQ +FE+KQA++F+ALDKLF LHNAMLLESR+IK F IYF SIF+IYMF+S
Sbjct: 391 FENSKSMLEAQVAFEAKQANMFVALDKLFALHNAMLLESRVIKAFVIYFLSIFVIYMFTS 450

Query: 436 TKQTYAVRPWLYIELCATFIFEVAILRLEVFDIEQQTWIINLVRIVFLFLAFFQLLHAIF 495
           TKQTY +RP LYI LC T   EVA LR  V D E+Q W+INL+R +F  LA  QLLHA  
Sbjct: 451 TKQTYIIRPRLYIGLCVTLALEVASLRY-VNDTERQAWMINLIRSLFALLASAQLLHAAL 510

Query: 496 TYRDYDALNYHMLLMLTERINEMQTPNKLSWGSD--SDLNWSSWIDTELSEDVEDL---D 555
           +YRDY+ LN+ +LL L +++N+MQ+  +LS+  D  S+++W+SW+DT+L++D ++L   D
Sbjct: 511 SYRDYEVLNHQILLRLVDKVNDMQSKKELSYDEDTESEVDWTSWVDTDLTDDDDNLADPD 570

Query: 556 YVLPEEIGENSITTTSTTRK-YNLRRR 571
           Y +P  I +N +TT+S TR+ YN R R
Sbjct: 571 YKIPLLIKDNPVTTSSLTRRLYNFRPR 593

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q681K76.4e-14550.09Protein GAMETE EXPRESSED 1 OS=Arabidopsis thaliana OX=3702 GN=GEX1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022143299.14.6e-25781.60protein GAMETE EXPRESSED 1 [Momordica charantia] >XP_022143300.1 protein GAMETE ... [more]
XP_038905677.11.6e-24979.63protein GAMETE EXPRESSED 1 [Benincasa hispida] >XP_038905679.1 protein GAMETE EX... [more]
XP_022983403.13.3e-24778.50protein GAMETE EXPRESSED 1 [Cucurbita maxima][more]
XP_023529107.12.8e-24678.33protein GAMETE EXPRESSED 1 [Cucurbita pepo subsp. pepo][more]
XP_022935308.12.8e-24678.33protein GAMETE EXPRESSED 1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1CNX62.2e-25781.60protein GAMETE EXPRESSED 1 OS=Momordica charantia OX=3673 GN=LOC111013205 PE=4 S... [more]
A0A6J1J7N61.6e-24778.50protein GAMETE EXPRESSED 1 OS=Cucurbita maxima OX=3661 GN=LOC111482011 PE=4 SV=1[more]
A0A6J1FA681.3e-24678.33protein GAMETE EXPRESSED 1 OS=Cucurbita moschata OX=3662 GN=LOC111442231 PE=4 SV... [more]
A0A5A7TSK82.1e-24477.66Protein GAMETE EXPRESSED 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BZN22.1e-24477.66protein GAMETE EXPRESSED 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494785 PE... [more]
Match NameE-valueIdentityDescription
AT5G55490.14.6e-14650.09gamete expressed protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 227..276
NoneNo IPR availableCOILSCoilCoilcoord: 147..181
NoneNo IPR availablePANTHERPTHR33538:SF2PROTEIN GAMETE EXPRESSED 1coord: 5..570
IPR040346GEX1/BrambleberryPANTHERPTHR33538PROTEIN GAMETE EXPRESSED 1coord: 5..570

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0010176.1Tan0010176.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane