Tan0010162 (gene) Snake gourd v1

Overview
NameTan0010162
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Description(+)-gamma-cadinene synthase-like
LocationLG01: 17453509 .. 17458358 (-)
RNA-Seq ExpressionTan0010162
SyntenyTan0010162
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGTTTCCTCAATTTTCAGTTGTTTCTTCTTCAAAGTCTCCAGACATCTCTCACGTCAAAAGTCGACCCACTGCAAAATTTCACCCCAGCATTTGGAAGGATCATTTCCTTTCTTATAGTTCCGAAGCCTTGGTAATTCAATTCATCTTTATATATATGTGTGTGTGCCTATTTTTTTAAATAATAAATTCTCACCTTTTTAAAAAATATCGATTTTATACTTTTTGTTTTTTGTTTTTATTATCAAATATTTACCATCATGAAATGTTAAAATAACGATGGTGTAGAGACTAAAAATGTTCTTTTTCTAAGATGACTTTATTGTTATCATGTAACTGTGTGTTATGCATTAAGCTGATGACTTTTAAATCAAGTAAACTATTTCTTGAGCTTATCACAATATATTATTATGGATGCAGACGCAGTCATATGAAGTGAGGAGGTTTTCTAATTTTATGGGTTAAAATGTCTGCTTATTGAATTCAAATAAAATGATATTTTTCTATCCAAATGATAAGTTTTTGAAATGTCACGTCATTATTTTATTAATCAATTTTAACTTTTGTTGACCTATTAATTTTTTCATAAGTGGTATTTCCATGAACGGTCATAGAACATAAAATTATGTTTTCAAATCTAGCAAAATTTTGCAAAATTATCACAATTAAAATCCAACCTAAAAATTTTGCAAAGAAATATGTATACATATATATACCTAAATTGAAATATAACCTGAAACACAATAATAAAAAACACACTCGTGCTTATTTGAAAAAAAGAACAAAAACTTCCAAATGATGTTTAGAAAGCATATTAAATTTGTGCTATTTAGTTTGTTTTGCATAATATATCATGATCATTCGCGAGGTACTTCATTTCAATTTTTCATTTTGATTTTGAGCTTTTTTTTTTTTTTTTAGATAATAGAAGACAAAATGATAGAACAAGTTGAGCAACAAAAGAACGAAGTAAGAAAGATGCTATTTGTTGTCATGAGAAACCCATTGGAGAAGTTAAAGTTAGTTGATACAATTCAACGCTTAGGTATATCTTATCATTTTGGAAATGATATTGACCAAGTCTTGGAGGAAATATATATGGCTTACAACGACTTATTGGATAAGTACAACGATGAAGATCTTAATGTTGTTGCTCTTCTATTTCGATTGCTCAGACAACAAGGCTATAAAATTTCATGTGGTAAGTTATGCATGCTTCAAATTATATAATGTTAAATAATGAAATAACCTTTAGAAATCTAACTCTTATATTTTAATATACATACCTAGTCATTAATGTTATCAGCAATAAAAAGTATGAAAATGCAAGATCAACACATTCAATGCTCAAAATGAAAAAGAAAAATTAATCTTTAGAATTGGAAAGGGAAGGTTTTCTCCCTCTACTAGAACATTTTCTCAAAGTTATGCAAGTATATATAGAAATTAAACAACCATTAAAGAATGCCTAACAAATTTTTTTTCTCTCAAAATTGAAATAGATATATTCACAAAGTTTACAAAAAATGGAAAATTCAAGGAGTCATTGGTCAGTGATGCAAGAGGAATTTTAAGCCTGCTTAAGCCTGTATGAAGCAGCACATATGAGAATCCATGGAGAAGATTTACTTGAAGAAGCACTTGAATTTACCATCAAGCATCTTAAAAACCTAATTGCCAATTCAAGCTCTTGGTTTATGATTGAAGTACAAAGTGCCCTAAAATGTCCTCTAAGATTGGCCCTTCCAAGAATAAAGGCTAGAGAGTACATGCTAATCTATGAACAAGATCCTTCACACAGTGATATTTTGCTTACTTTCTCAAAATTAGATTTCAACATATTACAAAAACGCCACCCGAAGGAGTTGGGTGCAATAACAAGGTACAAATATATTTTGATGATCATACTATTATTTCATTTTGTCTGTCTCAAGCACATGCTAGATTACCTTTGCTAAGATTATTTGATAATTAAGTTATTTAGACATTAAATTCTAAAATGTTAAATACATCATCAATCGAATTTCTAAATAGTGTTTATTGTTTACCATATTAACTAAATGATTATTTTTATTTTTGGAATTGGAATGAGAATTCAAATGTTTAGTTAAGAAATAATATTCAATGTTGAATGACTAAATTAAAGTTTAACCCTTAAGGGACTGTTTGGGGCGTTGAGTGGGTTATAATAACAGGGAGTTATAATAATCTGTGGGTTATTATAGTCTGTGAAACATATAATATTATTTAAAATGCAGAGTAGTATAGTATGAGGTTATAATAGTCTGTGTTTGGGGTGCAGAGTATTTCACAAGTTATTATAACACGGGTTATAATAACCAGTAGGCCAAACAAGCCCTAAAAGTTCTCTATAAAGGTCCCGATAATATCATTCTGAGTACATATACATAAATTTTAGTAAAAGATATCACAAATATTTGTAACATGATATTCAAATGATACTCTTAAACACGTCATATCGCATGATCGTTTTTTAACTATGAAAAGTTGCATATTATCATGAATTAGGTACTGCAATATTTATTGATCACTACACATAAACTTGAAAAACTATAAATATTTTTGGTTCTGAAAATGTCTCTACTTTAGACCAAGAATGCTCACATAAATGGATGTTATTAGATCATTATTGGAGGCAAAAAGTTAGATGTGTTGCATTTCTTAAAAATATATATTTTAATATACATTAAAAATAATCTTGGTGGTTGTACTCAAAAGGAAAAAAAAAAAGTCTGGTTGGTTATTATTTGAATGATTGATTATTATATTTATGTCTTATAAACTCAGGTGGTGGAAGGCTTTGGATATCCCAACAAACTTTCCTTTTGCAAGAGATAGAATTGTGGAGTTATACTTCTGGGTATTGGGTGCATATTTTGAACCTCATTTCAATGTTGCAAGAGAAATATTGACAAAAGTAATAGGCATGATATCAATTTTAGATGATATATATGATGCTTATGGAACGTAGGAAGAACTTCAAGTCTTCACTGCAGCAATACAAAGGTTTATTTTCCTTTTTTTTTCTTTTTTTTTTACGGTTACTTGTTTACTTAGTTTTAGTTTAAGTTTTTTTTTTCTATTAAAAAATAAAATAAAAGTCTAAGGTGAACAAATTAAAATACAATTATTAGACTATGTTGATATAATTAAATTTACTCCAAACTATAACTATTTAGTATTGACATTTCAAAAGAGACATTTTAATACTTAATGTTTGGAAAATGATTTTAATTTGGTCTTTTTGAGTTGATCATTACTTTTTATTAGGGATAACGACCCTTCACATATCTCCACATTGGTATGATATTATCCACTTTGGGCATAAACCCTCATGGCTTTACTTTTGGTTTCACCCAAAAGGCCTCATACCAATGGAGATACTATCCCTCACTTATATACCCATGATTTTCCCCTTCTCTAGCCTATGTGAGACTTTGGTCGCACTTCCAACAATCCTCCCCTCGAACAAAGTATCAACCGAGCCTCTCCTCAAATAGTCATCCATCCGTCTAGCTCTTATCCTGGCCAACTCCTTTACCCGGAGCACACCGCCTATCCGATAGAGCTCTCTACCATGGATCTCCACCATGACTACACTTCAAGGCTCGCCTCCTATGTGTCGGCACTCGAGAATTCTATCGATCACGACTCAGGGGCATGGTTCTGATACCACTGTTATGAACAACAGATCTCCACATATCTTCACAATGGTATGATATTGTCCACTTTGGGCATAAGCTCTCATGATGTGACATCTCAAAATTAATTGAGAATAAAAGAAGTAGCTCATCGTGAAATCTACTCATTTTTCTAATGTGGAATCCTCAATACGGTTAAGACATTAAACTCAAATCTGAGCCCTTCTTGTAATTAGCATGGCTCTCATATTCCTGAATCATGTTAGAGAATTTTTTTAATTTGTAATAATGTCATTGAATTTTCAGGTGGGATATAGACGCGACTAATCTACTTCCTGAGTACATGAAGTTTTATTATATAGTTCTTTTAGATCTTTTTGAGGAAATTGGCAAAAAAATAGACAAGGATGGAAGTTTGTACCACATATACTTTGCAAAAGAAGCGGTAAAGTTTCTTTCTATTTTTTTTTTTCTTCTTCTTTATTATGTGTAACACTTCTTATTGACTATACCTATTTCCTCTAATAGGTGAAAAAACTAGTGAAATCCTACCTCAAAGAAGCTGAGTGGTCAAGTAAAAAGTACAAACCAAATTTCAAGGAATATATGGAACTTGCATTAGACACTTCAGGTTATTCTGAGGTTGCAACCATTTCATTTCTTGGAATGGGTAACATTATAACAAAAGAGGTATTTGAATGGGTTTTCGATGGTCCTAAAATTATTGAAGCCTCAAAAATGATTTGTAGACTCATGGACGACATCGTTTCTCACAAGGTAGATATATAGACATACACACTAATTAATTAAAAAAAATATAATAGACCAATTAATTAAACAAAACTTAAGCTTTAATTTCCACTAACTTTGGTACTTCATTTTTCTTTCTTAGTTTGAGCAACAGAGAGAACATGTTGTGCCTGCTATAAATTGTTATATGGAGCAATATAATTGCTCAGAGGAAGAAGCGTGTGTTGAATTTCATAAGCAAATTGGAGATGCATGGAAGGACATAAATGAAGCCTGCTTTGATCGATTGATTGTTCCAAGACCTGTTTTGATGCGTGTATTAAATCTTTCTCGAGTTATTAACCTACTTTATAGGGAAGAGGATGGTTACACACATGGTAAAGGTCAAACTAAACTTTTTATCAAATCTCTACTTGTCGACCCTGTGCCTAATTGA

mRNA sequence

ATGTCGTTTCCTCAATTTTCAGTTGTTTCTTCTTCAAAGTCTCCAGACATCTCTCACGTCAAAAGTCGACCCACTGCAAAATTTCACCCCAGCATTTGGAAGGATCATTTCCTTTCTTATAGTTCCGAAGCCTTGATAATAGAAGACAAAATGATAGAACAAGTTGAGCAACAAAAGAACGAAGTAAGAAAGATGCTATTTGTTGTCATGAGAAACCCATTGGAGAAGTTAAAGTTAGTTGATACAATTCAACGCTTAGGTATATCTTATCATTTTGGAAATGATATTGACCAAGTCTTGGAGGAAATATATATGGCTTACAACGACTTATTGGATAAGTACAACGATGAAGATCTTAATGTTGTTGCTCTTCTATTTCGATTGCTCAGACAACAAGGCTATAAAATTTCATGTGCCTGCTTAAGCCTGTATGAAGCAGCACATATGAGAATCCATGGAGAAGATTTACTTGAAGAAGCACTTGAATTTACCATCAAGCATCTTAAAAACCTAATTGCCAATTCAAGCTCTTGGTTTATGATTGAAGTACAAAGTGCCCTAAAATGTCCTCTAAGATTGGCCCTTCCAAGAATAAAGGCTAGAGAGTACATGCTAATCTATGAACAAGATCCTTCACACAGTGATATTTTGCTTACTTTCTCAAAATTAGATTTCAACATATTACAAAAACGCCACCCGAAGGAGTTGGGTGCAATAACAAGGTGGTGGAAGGCTTTGGATATCCCAACAAACTTTCCTTTTGCAAGAGATAGAATTGTGGAGTTATACTTCTGGGTATTGGGTGCATATTTTGAACCTCATTTCAATGTTGCAAGAGAAATATTGACAAAAGTAATAGGCATGATATCAATTTTAGATGATATATATGATGCTTATGGAACGTGGGATATAGACGCGACTAATCTACTTCCTGAGTACATGAAGTTTTATTATATAGTTCTTTTAGATCTTTTTGAGGAAATTGGCAAAAAAATAGACAAGGATGGAAGTTTGTACCACATATACTTTGCAAAAGAAGCGGTGAAAAAACTAGTGAAATCCTACCTCAAAGAAGCTGAGTGGTCAAGTAAAAAGTACAAACCAAATTTCAAGGAATATATGGAACTTGCATTAGACACTTCAGGTTATTCTGAGGTTGCAACCATTTCATTTCTTGGAATGGGTAACATTATAACAAAAGAGGTATTTGAATGGGTTTTCGATGGTCCTAAAATTATTGAAGCCTCAAAAATGATTTGTAGACTCATGGACGACATCGTTTCTCACAAGTTTGAGCAACAGAGAGAACATGTTGTGCCTGCTATAAATTGTTATATGGAGCAATATAATTGCTCAGAGGAAGAAGCGTGTGTTGAATTTCATAAGCAAATTGGAGATGCATGGAAGGACATAAATGAAGCCTGCTTTGATCGATTGATTGTTCCAAGACCTGTTTTGATGCGTGTATTAAATCTTTCTCGAGTTATTAACCTACTTTATAGGGAAGAGGATGGTTACACACATGGTAAAGGTCAAACTAAACTTTTTATCAAATCTCTACTTGTCGACCCTGTGCCTAATTGA

Coding sequence (CDS)

ATGTCGTTTCCTCAATTTTCAGTTGTTTCTTCTTCAAAGTCTCCAGACATCTCTCACGTCAAAAGTCGACCCACTGCAAAATTTCACCCCAGCATTTGGAAGGATCATTTCCTTTCTTATAGTTCCGAAGCCTTGATAATAGAAGACAAAATGATAGAACAAGTTGAGCAACAAAAGAACGAAGTAAGAAAGATGCTATTTGTTGTCATGAGAAACCCATTGGAGAAGTTAAAGTTAGTTGATACAATTCAACGCTTAGGTATATCTTATCATTTTGGAAATGATATTGACCAAGTCTTGGAGGAAATATATATGGCTTACAACGACTTATTGGATAAGTACAACGATGAAGATCTTAATGTTGTTGCTCTTCTATTTCGATTGCTCAGACAACAAGGCTATAAAATTTCATGTGCCTGCTTAAGCCTGTATGAAGCAGCACATATGAGAATCCATGGAGAAGATTTACTTGAAGAAGCACTTGAATTTACCATCAAGCATCTTAAAAACCTAATTGCCAATTCAAGCTCTTGGTTTATGATTGAAGTACAAAGTGCCCTAAAATGTCCTCTAAGATTGGCCCTTCCAAGAATAAAGGCTAGAGAGTACATGCTAATCTATGAACAAGATCCTTCACACAGTGATATTTTGCTTACTTTCTCAAAATTAGATTTCAACATATTACAAAAACGCCACCCGAAGGAGTTGGGTGCAATAACAAGGTGGTGGAAGGCTTTGGATATCCCAACAAACTTTCCTTTTGCAAGAGATAGAATTGTGGAGTTATACTTCTGGGTATTGGGTGCATATTTTGAACCTCATTTCAATGTTGCAAGAGAAATATTGACAAAAGTAATAGGCATGATATCAATTTTAGATGATATATATGATGCTTATGGAACGTGGGATATAGACGCGACTAATCTACTTCCTGAGTACATGAAGTTTTATTATATAGTTCTTTTAGATCTTTTTGAGGAAATTGGCAAAAAAATAGACAAGGATGGAAGTTTGTACCACATATACTTTGCAAAAGAAGCGGTGAAAAAACTAGTGAAATCCTACCTCAAAGAAGCTGAGTGGTCAAGTAAAAAGTACAAACCAAATTTCAAGGAATATATGGAACTTGCATTAGACACTTCAGGTTATTCTGAGGTTGCAACCATTTCATTTCTTGGAATGGGTAACATTATAACAAAAGAGGTATTTGAATGGGTTTTCGATGGTCCTAAAATTATTGAAGCCTCAAAAATGATTTGTAGACTCATGGACGACATCGTTTCTCACAAGTTTGAGCAACAGAGAGAACATGTTGTGCCTGCTATAAATTGTTATATGGAGCAATATAATTGCTCAGAGGAAGAAGCGTGTGTTGAATTTCATAAGCAAATTGGAGATGCATGGAAGGACATAAATGAAGCCTGCTTTGATCGATTGATTGTTCCAAGACCTGTTTTGATGCGTGTATTAAATCTTTCTCGAGTTATTAACCTACTTTATAGGGAAGAGGATGGTTACACACATGGTAAAGGTCAAACTAAACTTTTTATCAAATCTCTACTTGTCGACCCTGTGCCTAATTGA

Protein sequence

MSFPQFSVVSSSKSPDISHVKSRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVRKMLFVVMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVVALLFRLLRQQGYKISCACLSLYEAAHMRIHGEDLLEEALEFTIKHLKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFSKLDFNILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREILTKVIGMISILDDIYDAYGTWDIDATNLLPEYMKFYYIVLLDLFEEIGKKIDKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVATISFLGMGNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQYNCSEEEACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTHGKGQTKLFIKSLLVDPVPN
Homology
BLAST of Tan0010162 vs. ExPASy Swiss-Prot
Match: B2KSJ5 ((+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 1.3e-176
Identity = 320/561 (57.04%), Postives = 404/561 (72.01%), Query Frame = 0

Query: 10  SSSKSPDISHVKSRPTAKFHPSIWKDHFLSYS-SEALIIEDKMIEQVEQQKNEVRKMLFV 69
           S  K+ DI  VK R  A FHP+IWK+HFLS++  +AL I++ M E+ E+ K E+R M+  
Sbjct: 11  SIMKTNDIPDVK-RSLANFHPNIWKEHFLSFTFDDALKIDEGMKERTEKLKEEIRMMMIA 70

Query: 70  VMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLL----DKYNDEDLNVVAL 129
            + N L KL LVD+IQRLG+SYHF +++D+ LE IY++YN+ L       N EDL++ AL
Sbjct: 71  YVENQLIKLNLVDSIQRLGVSYHFEDEVDEFLEHIYVSYNNSLLLSNKNSNGEDLHITAL 130

Query: 130 LFRLLRQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEALE 189
           LFRLLRQQGY+ISC                        LSLYEA+HM  HGE LLEEALE
Sbjct: 131 LFRLLRQQGYRISCDIFLKFMDDNGKFKESLVEDERGLLSLYEASHMMGHGEALLEEALE 190

Query: 190 FTIKHLKNLI---ANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLT 249
           FT  HL+  I   +N +  F  EV +ALK P+R ++PRIKAREY+ IY+Q PSH++ LL 
Sbjct: 191 FTTTHLQTYIHRYSNINPSFASEVSNALKLPIRKSVPRIKAREYLEIYQQHPSHNETLLE 250

Query: 250 FSKLDFNILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAR 309
           FSKLDFNILQK H KEL  I RWWK LD+PT FPFARDRIVE YFW LGAYFEP ++V R
Sbjct: 251 FSKLDFNILQKLHQKELSEICRWWKDLDVPTKFPFARDRIVECYFWTLGAYFEPQYSVGR 310

Query: 310 EILTKVIGMISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFE 369
           ++LTKVI + SILDDIYDAYGT             WD    + LP YMK +Y+ +L+L+E
Sbjct: 311 KMLTKVIAIASILDDIYDAYGTFEELQVLTPAIQRWDRSMVHTLPLYMKPFYVAMLELYE 370

Query: 370 EIGKKIDKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEV 429
           EIGK+IDKD +  H+  A   +K+L +SY +EA+W +K+YKP+FKEYMELAL T+GY+ +
Sbjct: 371 EIGKEIDKDQNSLHLQVAIGGIKRLSESYFEEAKWLNKEYKPSFKEYMELALKTTGYTML 430

Query: 430 ATISFLGMG-NIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCY 489
            +ISFLG+G +I+T EV +W+ +GP+II+AS +ICRLMDDI SHKFEQ+REHV  A+ CY
Sbjct: 431 ISISFLGLGDHIVTNEVLQWLSNGPQIIKASTIICRLMDDIASHKFEQEREHVASAVECY 490

Query: 490 MEQYNCSEEEACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDG 527
           M+QY+CSEEEAC+E HK++ DAWKD NEA +    VP PVLMRVLN SRVINLLY +EDG
Sbjct: 491 MKQYDCSEEEACIELHKEVVDAWKDTNEAFYRPFNVPVPVLMRVLNFSRVINLLYLDEDG 550

BLAST of Tan0010162 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 3.6e-150
Identity = 274/557 (49.19%), Postives = 362/557 (64.99%), Query Frame = 0

Query: 5   QFSVVSSSKSPDISHVKSRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVRK 64
           Q S V  + S ++S    R  A FHPSIW DHFLSY+SE    +D + + V+Q K EVRK
Sbjct: 4   QSSGVLLAPSKNLSPEVGRRCANFHPSIWGDHFLSYASEFTNTDDHLKQHVQQLKEEVRK 63

Query: 65  MLFVVMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVVAL 124
           ML     +  +KL L+D IQRLG++YHF ++ID+VL+ ++    D      +ED+   +L
Sbjct: 64  MLMAADDDSAQKLLLIDAIQRLGVAYHFESEIDEVLKHMF----DGSVVSAEEDVYTASL 123

Query: 125 LFRLLRQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEALE 184
            FRLLRQQGY +SC                        LSLYEA H R+HGED+L+EAL 
Sbjct: 124 RFRLLRQQGYHVSCDLFNNFKDNEGNFKESLSSDVRGMLSLYEATHFRVHGEDILDEALA 183

Query: 185 FTIKHLKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFSK 244
           FT  HL++   +SS+    +V  ALK P+R  LPR++AR Y  +Y+ D SH+  LL  +K
Sbjct: 184 FTTTHLQSATKHSSNPLAEQVVHALKQPIRKGLPRLEARHYFSVYQADDSHNKALLKLAK 243

Query: 245 LDFNILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREIL 304
           LDFN+LQK H KEL  I+ WWK LD     PFARDR+VE YFW+LG YFEP F  AR IL
Sbjct: 244 LDFNLLQKLHQKELSDISAWWKDLDFAHKLPFARDRVVECYFWILGVYFEPQFFFARRIL 303

Query: 305 TKVIGMISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFEEIG 364
           TKVI M SI+DDIYD YGT             WDI A + LPEYM+  Y  LL ++ EI 
Sbjct: 304 TKVIAMTSIIDDIYDVYGTLEELELFTEAVERWDISAIDQLPEYMRVCYQALLYVYSEIE 363

Query: 365 KKIDKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVATI 424
           +++ K+G  Y +Y+AKEA+K  V++Y +EA+W   +  P  +EYM +AL TS YS +AT 
Sbjct: 364 EEMAKEGRSYRLYYAKEAMKNQVRAYYEEAKWLQVQQIPTMEEYMPVALVTSAYSMLATT 423

Query: 425 SFLGMGNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQY 484
           SF+GMG+ +TKE F+W+F  PKI+ AS ++CRLMDD+V HKFEQ+R HV  A+ CYM+Q+
Sbjct: 424 SFVGMGDAVTKESFDWIFSKPKIVRASAIVCRLMDDMVFHKFEQKRGHVASAVECYMKQH 483

Query: 485 NCSEEEACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTHG 527
             SE+E   EF + + +AWKDINE C     VP P+LMRVLNL+RVI+++Y+ EDGYTH 
Sbjct: 484 GASEQETPNEFPQPVREAWKDINEECLIPTAVPMPILMRVLNLARVIDVIYKNEDGYTHF 543

BLAST of Tan0010162 vs. ExPASy Swiss-Prot
Match: B5A435 (Sesquiterpene synthase OS=Santalum album OX=35974 PE=1 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 7.7e-145
Identity = 266/553 (48.10%), Postives = 362/553 (65.46%), Query Frame = 0

Query: 9   VSSSKSPDISHVKSRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVRKMLFV 68
           V  S  P++  + SRP A F PSIW D F++Y+ E    + +   QVE+ K +VR+ L  
Sbjct: 6   VPISSVPNLKEL-SRPIANFPPSIWGDRFINYACEDENEQAQKERQVEELKEQVRRELAA 65

Query: 69  VMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYM-AYNDLLDKYNDEDLNVVALLFR 128
            +  PL++L ++D  QRLGI+YHF N+I++ LE IY+  Y +        DL  VAL FR
Sbjct: 66  TVDKPLQQLNIIDATQRLGIAYHFENEIEESLEHIYLHTYVENNCFQGSHDLYSVALWFR 125

Query: 129 LLRQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEALEFTI 188
           LLRQ GYK+SC                        L LYEA H+ +HGE++L++ALEF  
Sbjct: 126 LLRQDGYKVSCDVFDKFRDYEDNFKNSLMEDAKGLLELYEATHLSVHGEEMLDDALEFAK 185

Query: 189 KHLKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFSKLDF 248
             L++++ + +     +V+ AL  PLR  LPR++A  +  IYE   SH+  LL  +KLDF
Sbjct: 186 TRLESIVNHLNYPLAEQVRHALYRPLRKGLPRLEAVYFFRIYEAYDSHNKALLKLAKLDF 245

Query: 249 NILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREILTKV 308
           N+LQ  H KEL  + RWWK+LD    FPFARDR+VE YFWVLG YFEP +++AR+I+ KV
Sbjct: 246 NLLQSLHKKELSDMARWWKSLDFAAKFPFARDRLVEGYFWVLGVYFEPQYSLARKIIIKV 305

Query: 309 IGMISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFEEIGKKI 368
             MIS +DDIYDAYGT             WD+ + + LPEYMK  Y  +LD++ EI +++
Sbjct: 306 FTMISTIDDIYDAYGTLDELELFTKAMQRWDVGSLDQLPEYMKPCYKSILDVYNEIEEEM 365

Query: 369 DKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVATISFL 428
           D  GSL+ +++AKE +KKLV+ Y+ EA+W  +KY P F+EYM +AL TSGY+ + TIS+L
Sbjct: 366 DNQGSLFRMHYAKEVMKKLVEGYMDEAKWCHEKYVPTFEEYMPVALVTSGYTFLTTISYL 425

Query: 429 GMGNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQYNCS 488
           GMG I +KE F+W+F  P +IEAS+ +CRLMDD+ SHKFEQ+R HV   I CYM+QY  +
Sbjct: 426 GMGEIASKEAFDWLFSHPPVIEASESVCRLMDDMRSHKFEQERGHVASGIECYMKQYGVT 485

Query: 489 EEEACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTHGKGQ 526
           EEEA  EF KQ+  AWKDINE C     VP+P+LMR+LNL+RVI+++Y+ EDGYTH K  
Sbjct: 486 EEEAHDEFRKQLVKAWKDINEECLRPYRVPKPLLMRILNLTRVIDVIYKNEDGYTHVKKA 545

BLAST of Tan0010162 vs. ExPASy Swiss-Prot
Match: E3W207 (Sesquiterpene synthase OS=Santalum austrocaledonicum OX=293154 PE=2 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 2.5e-143
Identity = 266/553 (48.10%), Postives = 358/553 (64.74%), Query Frame = 0

Query: 9   VSSSKSPDISHVKSRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVRKMLFV 68
           V  S  P++  + SRP A F PSIW D F++Y+ E    + +   QVE+ K +VR+ L  
Sbjct: 6   VPISSVPNLKEL-SRPIANFPPSIWGDRFINYTCEDENEQAQKERQVEELKEQVRRELAA 65

Query: 69  VMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYM-AYNDLLDKYNDEDLNVVALLFR 128
            +  PL++L ++D  QRLGI+YHF N+I++ LE IY+  Y          DL  VAL FR
Sbjct: 66  TVDKPLQQLNIIDATQRLGIAYHFENEIEESLEHIYLHTYVKNNCFQGSHDLYSVALWFR 125

Query: 129 LLRQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEALEFTI 188
           LLRQ GYK+SC                        L LYEA H+ +HGE++L++ALEF  
Sbjct: 126 LLRQDGYKVSCDVFDNFRDYEGNFKNSLMEDAKGLLELYEATHLSVHGEEMLDDALEFAK 185

Query: 189 KHLKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFSKLDF 248
             L++++ + +     +V+ AL  PLR  LPR++A  +  IYE   SH+  LL  +KLDF
Sbjct: 186 TRLESIVNHLNYPLAEQVRHALYRPLRKGLPRLEAVYFFRIYEAYHSHNKALLKLAKLDF 245

Query: 249 NILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREILTKV 308
           N+LQ  H KELG + RWW++LD  T FPFARDR+VE YFW+LG YFEP +++AREI TKV
Sbjct: 246 NLLQSLHRKELGDMARWWRSLDFATKFPFARDRLVEGYFWILGVYFEPQYSLAREITTKV 305

Query: 309 IGMISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFEEIGKKI 368
             MIS +DDIYDAYGT             WD+ + + LPEYMK  Y  +LD++ EI +++
Sbjct: 306 FAMISTIDDIYDAYGTLDELKLFTEAIQRWDVGSLDQLPEYMKPCYKSILDVYNEIEEEM 365

Query: 369 DKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVATISFL 428
              GSL+ +++AKE +K +V+ Y+ EA+W  +KY P F+EYM LAL TSGY+ + TIS+L
Sbjct: 366 ANQGSLFRMHYAKEVMKTIVEGYMDEAKWCHEKYVPTFQEYMSLALVTSGYTFLTTISYL 425

Query: 429 GMGNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQYNCS 488
           GMG I +KE FEW+F  P IIEAS+ ICRLMDD+ SHKFEQ+R HV   I CYM+QY   
Sbjct: 426 GMGGIASKEAFEWLFSHPPIIEASESICRLMDDMSSHKFEQERGHVASGIECYMKQYGVI 485

Query: 489 EEEACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTHGKGQ 526
           EEEA  EFHK++  AWKDINE       VP P+LMR+LNL+RV++++Y+ ED YTH K  
Sbjct: 486 EEEAHDEFHKRLVKAWKDINEGFLRPYAVPEPLLMRILNLTRVMDVIYKNEDSYTHVKKA 545

BLAST of Tan0010162 vs. ExPASy Swiss-Prot
Match: F6M8H5 (Probable sesquiterpene synthase OS=Santalum austrocaledonicum OX=293154 GN=SesquiTPS PE=3 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 3.9e-141
Identity = 263/561 (46.88%), Postives = 363/561 (64.71%), Query Frame = 0

Query: 1   MSFPQFSVVSSSKSPDISHVKSRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKN 60
           M   +  + S S   D++ + SRP A F PSIW D F++Y+ E    + +  +QVE+ K 
Sbjct: 1   MENQKMPISSVSNLKDLNMI-SRPVANFPPSIWGDRFINYACEDENDQAQKEKQVEELKE 60

Query: 61  EVRKMLFVVMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYM-AYNDLLDKYNDEDL 120
            VR+ L + +  PL++L ++D  QRLGI+Y F N+I++ LE IY+  Y +       +DL
Sbjct: 61  LVRRELAITVDKPLQQLNIIDATQRLGIAYLFENEIEESLEHIYLHTYVENTCFEGSDDL 120

Query: 121 NVVALLFRLLRQQGYKISC----------------------ACLSLYEAAHMRIHGEDLL 180
             VAL FRLLRQ  Y++SC                        L LYEA H+ +HGE++L
Sbjct: 121 YSVALWFRLLRQNDYRVSCDVFNKFRDNEGNFKNNLMEDTKGLLELYEATHLSVHGEEML 180

Query: 181 EEALEFTIKHLKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDIL 240
           ++ALEFT   L++++++ +     +V+ AL  PL   LPR++A  +  IYE   SH+  L
Sbjct: 181 DDALEFTKTRLESVVSHLNYPLAEQVRHALYQPLHKGLPRLEAVYFFRIYEAHASHNKAL 240

Query: 241 LTFSKLDFNILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNV 300
           L  +KLDFN+LQ  H KEL  I RWWK+LD    FPFARDR+VE YFWVLG YFEP +++
Sbjct: 241 LKLAKLDFNLLQSFHKKELSDIARWWKSLDFAAKFPFARDRLVEGYFWVLGVYFEPQYSL 300

Query: 301 AREILTKVIGMISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDL 360
           AR+I+ KV  MIS +DDIYDAYGT             WD+ + + LPEYMK  Y  +LD+
Sbjct: 301 ARKIIIKVFTMISTIDDIYDAYGTLDELELFTKAMQRWDVGSLDQLPEYMKPCYKSILDV 360

Query: 361 FEEIGKKIDKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYS 420
           + EI  ++D  GSL+ +++AKE +KKLV+ Y+ EA+W  +KY P F+EYM +AL TSGY+
Sbjct: 361 YNEIEVEMDNQGSLFRMHYAKEVMKKLVEGYMDEAKWCHEKYVPTFQEYMSVALVTSGYT 420

Query: 421 EVATISFLGMGNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINC 480
            + TIS+LGMG I +KE F+W+F  P +IEAS+ + RLMDD  SHKFEQ+R HV   I C
Sbjct: 421 FLTTISYLGMGEIASKEAFDWLFSHPPVIEASESVGRLMDDTRSHKFEQERGHVASGIEC 480

Query: 481 YMEQYNCSEEEACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREED 526
           YM+QY  +EEEA  EF K++  AWKDINE C     VP+P+LMR+LNL+RVI+++Y+ ED
Sbjct: 481 YMKQYGVTEEEARDEFRKRLVKAWKDINEECLRPYRVPKPLLMRILNLTRVIDVIYKNED 540

BLAST of Tan0010162 vs. NCBI nr
Match: XP_022142732.1 ((+)-gamma-cadinene synthase-like [Momordica charantia])

HSP 1 Score: 712.6 bits (1838), Expect = 2.5e-201
Identity = 353/557 (63.38%), Postives = 428/557 (76.84%), Query Frame = 0

Query: 4   PQFSVVSSSKSPDISHVKSRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVR 63
           PQ S  S+ K+P +S+ K RP A FHPSIWKDHFL+   EAL +ED++ E+ EQ K EVR
Sbjct: 3   PQVS-ASNMKAPHVSNTKHRPIASFHPSIWKDHFLAPIYEALEVEDEVKEEREQLKEEVR 62

Query: 64  KMLFVVMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVVA 123
           KML VV+ NP +KLKLVD IQ+LG+ YHF  +I+QVLE +Y+ Y + L+ + DEDLN V+
Sbjct: 63  KMLIVVVENPFDKLKLVDWIQKLGVFYHFEKEINQVLEHMYVTYKNFLNDFGDEDLNTVS 122

Query: 124 LLFRLLRQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEAL 183
           LLFR+LRQQGY+ISC                        LSLYEAAH+RIHGED+LE+AL
Sbjct: 123 LLFRMLRQQGYRISCDIFSKFMKNDGQFKESLRGDTRGILSLYEAAHLRIHGEDILEKAL 182

Query: 184 EFTIKHLKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFS 243
           +FT  HLK L+A+S+S F  E+++ALK P+R ALPR+KAR+YM+IY+QDPSH+D LLTFS
Sbjct: 183 DFTTNHLKKLVADSTSPFTTEIKNALKWPIRKALPRVKARDYMVIYQQDPSHNDALLTFS 242

Query: 244 KLDFNILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREI 303
           KLDFNILQK H  EL AITRWWK LD+P   PFARDRIVE YFW+LG YFEPHF++AR+I
Sbjct: 243 KLDFNILQKLHQSELNAITRWWKDLDLPRTLPFARDRIVECYFWILGVYFEPHFSIARKI 302

Query: 304 LTKVIGMISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFEEI 363
           LTKV  M SILDD+YDAYGT             WDI   NLLPEYMK YY  LL+L+EEI
Sbjct: 303 LTKVTAMTSILDDMYDAYGTYEELEILTLAIKRWDISMVNLLPEYMKVYYKTLLELYEEI 362

Query: 364 GKKIDKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVAT 423
           G+ I KDG  Y I+FAKEA+K+L +SY KEAEW SK+Y+PNF EYME+ALD+SGY+ +AT
Sbjct: 363 GRDIGKDGKSYQIHFAKEAMKRLAESYFKEAEWLSKRYRPNFDEYMEVALDSSGYAVLAT 422

Query: 424 ISFLGMGNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQ 483
           ISF+GMG+I T+EVF+W+   PKIIEAS MI RLMDD++S+KFEQQREHVV A+ CYMEQ
Sbjct: 423 ISFVGMGDIATREVFDWLSKHPKIIEASTMISRLMDDVMSYKFEQQREHVVSAVECYMEQ 482

Query: 484 YNCSEEEACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTH 526
           Y CSEEEACVEF+KQ+ +AWKDINEAC     VP   LMRVLNLSRV+ LLY +EDGYT+
Sbjct: 483 YGCSEEEACVEFNKQVAEAWKDINEACLHPTTVPMIFLMRVLNLSRVMALLYVDEDGYTN 542

BLAST of Tan0010162 vs. NCBI nr
Match: XP_023520451.1 (LOW QUALITY PROTEIN: (+)-gamma-cadinene synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 676.8 bits (1745), Expect = 1.5e-190
Identity = 340/558 (60.93%), Postives = 417/558 (74.73%), Query Frame = 0

Query: 5   QFSVVSSSKSPDISHVK-SRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVR 64
           Q S+VS+      +  K  RP AK+HPSIWKDHFLS +S+AL ++ +M +QV+Q K EV+
Sbjct: 4   QISLVSTPTIKSSNTFKGKRPVAKYHPSIWKDHFLSLTSDALSVDIEMEKQVKQLKVEVK 63

Query: 65  KMLFVVMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVVA 124
           KML +  RN LEKL LVD+IQRLG+SYHF N+IDQVLE+IY  Y D      DEDL+ V+
Sbjct: 64  KMLMISNRNLLEKLSLVDSIQRLGVSYHFENEIDQVLEKIYANYRDFTSIDGDEDLHTVS 123

Query: 125 LLFRLLRQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEAL 184
           LLFRLLRQQGYKI C                        LSLYEAAHMR+HGED+LEEAL
Sbjct: 124 LLFRLLRQQGYKIPCDIFTKFVESNGKFKESLTEDARGILSLYEAAHMRVHGEDILEEAL 183

Query: 185 EFTIKHLKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFS 244
           +F++K+L+++  NS+  F  EVQSALK PLR  LPR+KAR Y+ +Y++DPSHS  LL FS
Sbjct: 184 DFSVKYLRDVTTNSNPKFADEVQSALKWPLRKTLPRLKARAYVTMYQEDPSHSQTLLAFS 243

Query: 245 KLDFNILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREI 304
           KLDFN+LQK H KEL  +TRWWK L++PTNFPFARDRIVE YFW LGAYFEPHF V R+I
Sbjct: 244 KLDFNVLQKLHRKELKELTRWWKDLEVPTNFPFARDRIVESYFWTLGAYFEPHFGVGRKI 303

Query: 305 LTKVIGMISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFEEI 364
           LTKVI + SILDDIYDAYGT             WD    N+LP+YMK +YI +LD+FEEI
Sbjct: 304 LTKVIVISSILDDIYDAYGTFEELQIFTTAIQRWDRSMVNVLPKYMKHFYIAMLDVFEEI 363

Query: 365 GKKIDKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVAT 424
            K+I KD S  HI  A+E +KKL +SY KEA+W +KK KPNF EYMELAL ++GY+ + +
Sbjct: 364 SKEIGKDESSLHIRTAREGIKKLAQSYFKEAKWLNKKXKPNFNEYMELALCSTGYTFIIS 423

Query: 425 ISFLGMGNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQ 484
           ISFLGMG+I+T EV EW+   PKI++AS +ICRLMDD+VSHKFEQ+REHV  A+ CYMEQ
Sbjct: 424 ISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREHVTSAVECYMEQ 483

Query: 485 YNCSEEEACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTH 527
           Y CSE+EACVE HKQ+ DAWKDINEAC   + VP  +LMRV+NLSRVIN+LY +EDGYT+
Sbjct: 484 YGCSEKEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVINVLYLDEDGYTN 543

BLAST of Tan0010162 vs. NCBI nr
Match: XP_022932164.1 ((+)-gamma-cadinene synthase-like [Cucurbita moschata])

HSP 1 Score: 671.8 bits (1732), Expect = 4.9e-189
Identity = 337/558 (60.39%), Postives = 416/558 (74.55%), Query Frame = 0

Query: 5   QFSVVSSSKSPDISHVK-SRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVR 64
           Q S+VS+S     +  K  RP AK+HPSIWKDHFLS +S+ L I+ +M EQ ++ K EV+
Sbjct: 4   QISLVSASTIKSSNTFKGKRPVAKYHPSIWKDHFLSLTSDTLSIDVEMEEQAKRLKVEVK 63

Query: 65  KMLFVVMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVVA 124
           KML +  RN LEKL LVD+IQRLG+SYHF N+IDQVLE+IY  Y D      DEDL  V+
Sbjct: 64  KMLMISNRNLLEKLSLVDSIQRLGVSYHFENEIDQVLEKIYANYRDFTSIDGDEDLPTVS 123

Query: 125 LLFRLLRQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEAL 184
           LLFRLLRQQGYKI C                        L+LYEAAHMR+HGED+LEEAL
Sbjct: 124 LLFRLLRQQGYKIPCDIFTKFVESNGKFKESLAKDARGILNLYEAAHMRVHGEDILEEAL 183

Query: 185 EFTIKHLKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFS 244
           +F++K+L+++  NS+  +  EVQSALK  LR  LPR+KAREY+ +Y++DPSHS  LL FS
Sbjct: 184 DFSVKYLRDVRTNSNPKYADEVQSALKWSLRKTLPRLKAREYITMYQEDPSHSQTLLAFS 243

Query: 245 KLDFNILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREI 304
           KLDF+ILQK H +EL  +TRWWK L++PTNFPFARDRIVE YFW LGAYFEPHF+V R+I
Sbjct: 244 KLDFSILQKLHQRELKELTRWWKDLEVPTNFPFARDRIVESYFWTLGAYFEPHFSVGRKI 303

Query: 305 LTKVIGMISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFEEI 364
           LTKVI + SILDDIYDAYGT             WD    N LP+YMK +YI +LD+FEEI
Sbjct: 304 LTKVIALSSILDDIYDAYGTFEELQVFTTAIQRWDRSMVNELPKYMKHFYIAMLDVFEEI 363

Query: 365 GKKIDKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVAT 424
            K+I KD S  H+  A+E +KKL +SY +EA+W +KKYKPNF EYMELAL ++GY+ + +
Sbjct: 364 SKEIGKDESSLHVRTAREGIKKLAQSYFEEAKWLNKKYKPNFNEYMELALCSTGYTFIIS 423

Query: 425 ISFLGMGNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQ 484
           ISFLGMG+I+T EV EW+   PKI++AS +ICRLMDD+VSHKFEQ+REHV  A+ CYMEQ
Sbjct: 424 ISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREHVTSAVECYMEQ 483

Query: 485 YNCSEEEACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTH 527
           Y CSEEEACVE HKQ+ DAWKDINEAC   + VP  +LMRV+NLSRVIN+LY +EDGYT+
Sbjct: 484 YGCSEEEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVINVLYLDEDGYTN 543

BLAST of Tan0010162 vs. NCBI nr
Match: XP_023552098.1 ((+)-gamma-cadinene synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 665.2 bits (1715), Expect = 4.6e-187
Identity = 333/552 (60.33%), Postives = 407/552 (73.73%), Query Frame = 0

Query: 10  SSSKSPDISHVKSRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVRKMLFVV 69
           SS KSP+    K RP A FHPSIWKDHFLS SSEA  I+ ++ EQ +Q K EV+KML + 
Sbjct: 11  SSMKSPNNLKAK-RPIANFHPSIWKDHFLSLSSEATKIDVEVEEQAKQLKGEVKKMLIIS 70

Query: 70  MRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVVALLFRLL 129
            RNP + L LVD+IQRL +SYHF N+IDQ+LE+IY  Y +  +   ++DL  ++LLFRLL
Sbjct: 71  NRNPFKILNLVDSIQRLNVSYHFENEIDQILEQIYANYREFTNIDGNDDLYTISLLFRLL 130

Query: 130 RQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEALEFTIKH 189
           RQQGYKI C                      A LSLYEAAHMR+HGED+LEEAL+F++KH
Sbjct: 131 RQQGYKIPCDIFTKFVESNGKFKESLAENARAILSLYEAAHMRVHGEDVLEEALDFSVKH 190

Query: 190 LKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFSKLDFNI 249
           LKN+I+NSS  F+ EV SALK PLR A+PR+KAREY+ IYE+DPSHS  LLTFSKLDFNI
Sbjct: 191 LKNIISNSSPIFVAEVTSALKYPLRKAIPRMKAREYIPIYEEDPSHSKTLLTFSKLDFNI 250

Query: 250 LQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREILTKVIG 309
           LQK H KEL  + RWWK L + TNF FARDRIVE Y W LG YFEP F+V R++LTKV  
Sbjct: 251 LQKLHQKELIEVIRWWKDLKVETNFSFARDRIVECYLWTLGVYFEPQFSVGRKLLTKVTA 310

Query: 310 MISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFEEIGKKIDK 369
           + SILDDIYDAYGT             WD    N+LPEYMK +YI +LDL++EI K+IDK
Sbjct: 311 ISSILDDIYDAYGTFEELQVLTPAIQRWDRSMVNVLPEYMKPFYIAMLDLYKEISKEIDK 370

Query: 370 DGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVATISFLGM 429
           D S  H++ AKE +KKL +SY +EA+W SK YKPNFKE+MELAL T+GYS + +IS L M
Sbjct: 371 DESSLHVHIAKEEIKKLAESYFEEAKWLSKNYKPNFKEHMELALYTTGYSLLISISLLVM 430

Query: 430 GNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQYNCSEE 489
           GN++T +V +WV   PKIIE S +ICRLMDDIVSHKFEQ+REHV  A+ CY+EQY CSEE
Sbjct: 431 GNMVTNDVLQWVSRRPKIIEGSTIICRLMDDIVSHKFEQEREHVASAVECYIEQYGCSEE 490

Query: 490 EACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTHGKGQTK 527
           EACVE HKQ+ DAWKD+NEAC   + VP P+LMR +N SRV+N++Y +EDGYT+  G+TK
Sbjct: 491 EACVELHKQVVDAWKDVNEACLHPIAVPMPILMRTVNFSRVMNVIYSDEDGYTNSNGKTK 550

BLAST of Tan0010162 vs. NCBI nr
Match: XP_022985144.1 (LOW QUALITY PROTEIN: (+)-gamma-cadinene synthase-like [Cucurbita maxima])

HSP 1 Score: 658.3 bits (1697), Expect = 5.6e-185
Identity = 329/552 (59.60%), Postives = 409/552 (74.09%), Query Frame = 0

Query: 10  SSSKSPDISHVKSRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVRKMLFVV 69
           SS KSP+   VK RP A FH SIWKDHFLS SSEA+ I+ +M EQ ++ K +++KML + 
Sbjct: 16  SSMKSPNTLKVK-RPIANFHSSIWKDHFLSLSSEAMKIDGEMEEQAKEMKVKLKKMLLIC 75

Query: 70  MRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVVALLFRLL 129
            RNPL+ L LVD+IQRL +SYHF N+I Q+LE+IY  Y +  +  +  DL  ++LLFRLL
Sbjct: 76  NRNPLKILNLVDSIQRLNVSYHFENEIHQILEQIYENYREFTN-IDGXDLYTISLLFRLL 135

Query: 130 RQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEALEFTIKH 189
           RQQGYKI C                        L+LYEAAHMRIHGED+LEEAL+F++KH
Sbjct: 136 RQQGYKIPCDIFTKFVESNGKFKESLAEDVRGILNLYEAAHMRIHGEDVLEEALDFSVKH 195

Query: 190 LKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFSKLDFNI 249
           LKN+I+NSSS F+ EV+SALK PLR A+PR+KAREY+ IY++DPSHS  LLTFSKLDFN+
Sbjct: 196 LKNIISNSSSIFVAEVKSALKYPLRKAIPRMKAREYISIYQEDPSHSKTLLTFSKLDFNL 255

Query: 250 LQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREILTKVIG 309
           LQ+ H KEL  + RWWK L + TNF FARDRIVE Y W LG YFEP F+V R+ILTKV  
Sbjct: 256 LQRLHQKELIEVIRWWKDLKVETNFSFARDRIVECYLWTLGVYFEPQFSVGRKILTKVTA 315

Query: 310 MISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFEEIGKKIDK 369
           + SILDDIYDAYGT             WD    N+LPEYMK +YI +LD+++EI K+IDK
Sbjct: 316 ISSILDDIYDAYGTFEELQVITTAIQRWDRSMVNVLPEYMKPFYIAMLDVYKEISKEIDK 375

Query: 370 DGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVATISFLGM 429
           D S  HI+ AKE +KKL +SY KEA+W SK YKPNFKE+MELAL T+GYS + +IS LGM
Sbjct: 376 DESSLHIHVAKEEIKKLAESYFKEAKWLSKNYKPNFKEHMELALKTTGYSLLISISLLGM 435

Query: 430 GNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQYNCSEE 489
           GN++T +V +WV   PKII+ S +ICRLMDDIVSHKFEQ+REH+  A+ CY+EQY CSEE
Sbjct: 436 GNMVTNDVLQWVSRRPKIIKGSTIICRLMDDIVSHKFEQEREHIASAVECYIEQYGCSEE 495

Query: 490 EACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTHGKGQTK 527
           EACVE HKQ+ DAWKD+NEAC   + VP P+LMR +N SRV+N++Y +EDGYT+  G+TK
Sbjct: 496 EACVELHKQVVDAWKDVNEACLRPIAVPMPILMRTVNFSRVMNVIYSDEDGYTNSNGKTK 555

BLAST of Tan0010162 vs. ExPASy TrEMBL
Match: A0A6J1CNP6 ((+)-gamma-cadinene synthase-like OS=Momordica charantia OX=3673 GN=LOC111012779 PE=4 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 1.2e-201
Identity = 353/557 (63.38%), Postives = 428/557 (76.84%), Query Frame = 0

Query: 4   PQFSVVSSSKSPDISHVKSRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVR 63
           PQ S  S+ K+P +S+ K RP A FHPSIWKDHFL+   EAL +ED++ E+ EQ K EVR
Sbjct: 3   PQVS-ASNMKAPHVSNTKHRPIASFHPSIWKDHFLAPIYEALEVEDEVKEEREQLKEEVR 62

Query: 64  KMLFVVMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVVA 123
           KML VV+ NP +KLKLVD IQ+LG+ YHF  +I+QVLE +Y+ Y + L+ + DEDLN V+
Sbjct: 63  KMLIVVVENPFDKLKLVDWIQKLGVFYHFEKEINQVLEHMYVTYKNFLNDFGDEDLNTVS 122

Query: 124 LLFRLLRQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEAL 183
           LLFR+LRQQGY+ISC                        LSLYEAAH+RIHGED+LE+AL
Sbjct: 123 LLFRMLRQQGYRISCDIFSKFMKNDGQFKESLRGDTRGILSLYEAAHLRIHGEDILEKAL 182

Query: 184 EFTIKHLKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFS 243
           +FT  HLK L+A+S+S F  E+++ALK P+R ALPR+KAR+YM+IY+QDPSH+D LLTFS
Sbjct: 183 DFTTNHLKKLVADSTSPFTTEIKNALKWPIRKALPRVKARDYMVIYQQDPSHNDALLTFS 242

Query: 244 KLDFNILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREI 303
           KLDFNILQK H  EL AITRWWK LD+P   PFARDRIVE YFW+LG YFEPHF++AR+I
Sbjct: 243 KLDFNILQKLHQSELNAITRWWKDLDLPRTLPFARDRIVECYFWILGVYFEPHFSIARKI 302

Query: 304 LTKVIGMISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFEEI 363
           LTKV  M SILDD+YDAYGT             WDI   NLLPEYMK YY  LL+L+EEI
Sbjct: 303 LTKVTAMTSILDDMYDAYGTYEELEILTLAIKRWDISMVNLLPEYMKVYYKTLLELYEEI 362

Query: 364 GKKIDKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVAT 423
           G+ I KDG  Y I+FAKEA+K+L +SY KEAEW SK+Y+PNF EYME+ALD+SGY+ +AT
Sbjct: 363 GRDIGKDGKSYQIHFAKEAMKRLAESYFKEAEWLSKRYRPNFDEYMEVALDSSGYAVLAT 422

Query: 424 ISFLGMGNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQ 483
           ISF+GMG+I T+EVF+W+   PKIIEAS MI RLMDD++S+KFEQQREHVV A+ CYMEQ
Sbjct: 423 ISFVGMGDIATREVFDWLSKHPKIIEASTMISRLMDDVMSYKFEQQREHVVSAVECYMEQ 482

Query: 484 YNCSEEEACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTH 526
           Y CSEEEACVEF+KQ+ +AWKDINEAC     VP   LMRVLNLSRV+ LLY +EDGYT+
Sbjct: 483 YGCSEEEACVEFNKQVAEAWKDINEACLHPTTVPMIFLMRVLNLSRVMALLYVDEDGYTN 542

BLAST of Tan0010162 vs. ExPASy TrEMBL
Match: A0A6J1EW93 ((+)-gamma-cadinene synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111438481 PE=4 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 2.4e-189
Identity = 337/558 (60.39%), Postives = 416/558 (74.55%), Query Frame = 0

Query: 5   QFSVVSSSKSPDISHVK-SRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVR 64
           Q S+VS+S     +  K  RP AK+HPSIWKDHFLS +S+ L I+ +M EQ ++ K EV+
Sbjct: 4   QISLVSASTIKSSNTFKGKRPVAKYHPSIWKDHFLSLTSDTLSIDVEMEEQAKRLKVEVK 63

Query: 65  KMLFVVMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVVA 124
           KML +  RN LEKL LVD+IQRLG+SYHF N+IDQVLE+IY  Y D      DEDL  V+
Sbjct: 64  KMLMISNRNLLEKLSLVDSIQRLGVSYHFENEIDQVLEKIYANYRDFTSIDGDEDLPTVS 123

Query: 125 LLFRLLRQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEAL 184
           LLFRLLRQQGYKI C                        L+LYEAAHMR+HGED+LEEAL
Sbjct: 124 LLFRLLRQQGYKIPCDIFTKFVESNGKFKESLAKDARGILNLYEAAHMRVHGEDILEEAL 183

Query: 185 EFTIKHLKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFS 244
           +F++K+L+++  NS+  +  EVQSALK  LR  LPR+KAREY+ +Y++DPSHS  LL FS
Sbjct: 184 DFSVKYLRDVRTNSNPKYADEVQSALKWSLRKTLPRLKAREYITMYQEDPSHSQTLLAFS 243

Query: 245 KLDFNILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREI 304
           KLDF+ILQK H +EL  +TRWWK L++PTNFPFARDRIVE YFW LGAYFEPHF+V R+I
Sbjct: 244 KLDFSILQKLHQRELKELTRWWKDLEVPTNFPFARDRIVESYFWTLGAYFEPHFSVGRKI 303

Query: 305 LTKVIGMISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFEEI 364
           LTKVI + SILDDIYDAYGT             WD    N LP+YMK +YI +LD+FEEI
Sbjct: 304 LTKVIALSSILDDIYDAYGTFEELQVFTTAIQRWDRSMVNELPKYMKHFYIAMLDVFEEI 363

Query: 365 GKKIDKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVAT 424
            K+I KD S  H+  A+E +KKL +SY +EA+W +KKYKPNF EYMELAL ++GY+ + +
Sbjct: 364 SKEIGKDESSLHVRTAREGIKKLAQSYFEEAKWLNKKYKPNFNEYMELALCSTGYTFIIS 423

Query: 425 ISFLGMGNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQ 484
           ISFLGMG+I+T EV EW+   PKI++AS +ICRLMDD+VSHKFEQ+REHV  A+ CYMEQ
Sbjct: 424 ISFLGMGDIVTNEVLEWLSRRPKIVKASAVICRLMDDVVSHKFEQEREHVTSAVECYMEQ 483

Query: 485 YNCSEEEACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTH 527
           Y CSEEEACVE HKQ+ DAWKDINEAC   + VP  +LMRV+NLSRVIN+LY +EDGYT+
Sbjct: 484 YGCSEEEACVELHKQVVDAWKDINEACLYPIDVPMRILMRVVNLSRVINVLYLDEDGYTN 543

BLAST of Tan0010162 vs. ExPASy TrEMBL
Match: A0A6J1JCH1 (LOW QUALITY PROTEIN: (+)-gamma-cadinene synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111483232 PE=4 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 2.7e-185
Identity = 329/552 (59.60%), Postives = 409/552 (74.09%), Query Frame = 0

Query: 10  SSSKSPDISHVKSRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVRKMLFVV 69
           SS KSP+   VK RP A FH SIWKDHFLS SSEA+ I+ +M EQ ++ K +++KML + 
Sbjct: 16  SSMKSPNTLKVK-RPIANFHSSIWKDHFLSLSSEAMKIDGEMEEQAKEMKVKLKKMLLIC 75

Query: 70  MRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVVALLFRLL 129
            RNPL+ L LVD+IQRL +SYHF N+I Q+LE+IY  Y +  +  +  DL  ++LLFRLL
Sbjct: 76  NRNPLKILNLVDSIQRLNVSYHFENEIHQILEQIYENYREFTN-IDGXDLYTISLLFRLL 135

Query: 130 RQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEALEFTIKH 189
           RQQGYKI C                        L+LYEAAHMRIHGED+LEEAL+F++KH
Sbjct: 136 RQQGYKIPCDIFTKFVESNGKFKESLAEDVRGILNLYEAAHMRIHGEDVLEEALDFSVKH 195

Query: 190 LKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFSKLDFNI 249
           LKN+I+NSSS F+ EV+SALK PLR A+PR+KAREY+ IY++DPSHS  LLTFSKLDFN+
Sbjct: 196 LKNIISNSSSIFVAEVKSALKYPLRKAIPRMKAREYISIYQEDPSHSKTLLTFSKLDFNL 255

Query: 250 LQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREILTKVIG 309
           LQ+ H KEL  + RWWK L + TNF FARDRIVE Y W LG YFEP F+V R+ILTKV  
Sbjct: 256 LQRLHQKELIEVIRWWKDLKVETNFSFARDRIVECYLWTLGVYFEPQFSVGRKILTKVTA 315

Query: 310 MISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFEEIGKKIDK 369
           + SILDDIYDAYGT             WD    N+LPEYMK +YI +LD+++EI K+IDK
Sbjct: 316 ISSILDDIYDAYGTFEELQVITTAIQRWDRSMVNVLPEYMKPFYIAMLDVYKEISKEIDK 375

Query: 370 DGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVATISFLGM 429
           D S  HI+ AKE +KKL +SY KEA+W SK YKPNFKE+MELAL T+GYS + +IS LGM
Sbjct: 376 DESSLHIHVAKEEIKKLAESYFKEAKWLSKNYKPNFKEHMELALKTTGYSLLISISLLGM 435

Query: 430 GNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQYNCSEE 489
           GN++T +V +WV   PKII+ S +ICRLMDDIVSHKFEQ+REH+  A+ CY+EQY CSEE
Sbjct: 436 GNMVTNDVLQWVSRRPKIIKGSTIICRLMDDIVSHKFEQEREHIASAVECYIEQYGCSEE 495

Query: 490 EACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTHGKGQTK 527
           EACVE HKQ+ DAWKD+NEAC   + VP P+LMR +N SRV+N++Y +EDGYT+  G+TK
Sbjct: 496 EACVELHKQVVDAWKDVNEACLRPIAVPMPILMRTVNFSRVMNVIYSDEDGYTNSNGKTK 555

BLAST of Tan0010162 vs. ExPASy TrEMBL
Match: A0A6J1EZA9 (LOW QUALITY PROTEIN: (+)-gamma-cadinene synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111440345 PE=4 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 8.7e-184
Identity = 331/559 (59.21%), Postives = 410/559 (73.35%), Query Frame = 0

Query: 5   QFSVVSSS--KSPDISHVKSRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEV 64
           Q S+VS+S  KSPD    K RP A FHPSIWKD FLS SSEA+ I+ ++ EQ +Q K EV
Sbjct: 4   QTSLVSASSMKSPDNLKAK-RPIANFHPSIWKDQFLSLSSEAMKIDVEVEEQAKQFKGEV 63

Query: 65  RKMLFVVMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVV 124
           +KML    RN  + L LVD+IQRL +SYHF N+IDQ+LE+IY  Y +  +   ++DL  +
Sbjct: 64  KKMLISSNRNHFKILNLVDSIQRLNVSYHFENEIDQILEQIYANYREFTNIDGNDDLYTI 123

Query: 125 ALLFRLLRQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEA 184
           +LLFRLLRQQGYKISC                        LSLYEAAHMR+HGED LEEA
Sbjct: 124 SLLFRLLRQQGYKISCDIFTKFIESNGKFKESLAEDARGILSLYEAAHMRVHGEDXLEEA 183

Query: 185 LEFTIKHLKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTF 244
           L+F++KHLKN+I+NSS  F+ EV SALK PLR A+PR+KAREY+ IY++DPSHS  LLTF
Sbjct: 184 LDFSVKHLKNIISNSSPIFVAEVTSALKYPLRKAIPRMKAREYIPIYQEDPSHSKTLLTF 243

Query: 245 SKLDFNILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVARE 304
           SKLDFN+LQK H KEL  + RWWK L + TNF FARDRIVE Y W LG YFEP F+V R+
Sbjct: 244 SKLDFNVLQKLHQKELIEVIRWWKDLKVETNFSFARDRIVECYLWTLGVYFEPQFSVGRK 303

Query: 305 ILTKVIGMISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFEE 364
           +LTKV  + SILDDIYDAYGT             WD    N+LPEYMK +YI +LDL++E
Sbjct: 304 MLTKVTAISSILDDIYDAYGTFEELQVLTLAIQRWDRSMVNVLPEYMKPFYIAMLDLYKE 363

Query: 365 IGKKIDKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVA 424
           I K+IDKDGS  H++ AKE +KKL +SY +EA+W SK YKPNFKE++ELAL T+GYS + 
Sbjct: 364 ISKEIDKDGSSLHVHIAKE-IKKLAESYFEEAKWLSKNYKPNFKEHIELALYTTGYSLLI 423

Query: 425 TISFLGMGNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYME 484
           +IS LGMGN++T +V +WV   PKII+ S +ICRLMDDIVSHKFEQ+R+HV  A+ CY+E
Sbjct: 424 SISLLGMGNMVTNDVLQWVSRRPKIIKGSTIICRLMDDIVSHKFEQERKHVASAVECYIE 483

Query: 485 QYNCSEEEACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYT 527
           QY C EEEACVE HKQ+ DAWKD+NEAC   + VP P+LMR +N SRV+N++Y +EDGYT
Sbjct: 484 QYGCXEEEACVELHKQVVDAWKDVNEACLHPITVPMPILMRAVNFSRVMNVIYSDEDGYT 543

BLAST of Tan0010162 vs. ExPASy TrEMBL
Match: A0A6J1J7F9 ((+)-gamma-cadinene synthase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483269 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 3.7e-182
Identity = 323/559 (57.78%), Postives = 410/559 (73.35%), Query Frame = 0

Query: 5   QFSVVSSS--KSPDISHVKSRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEV 64
           Q S+VS+S  KSP+ S VK RP AK+HPSIWKDHFLS SSEA+ I+ +M E+ +Q K EV
Sbjct: 4   QTSLVSASTMKSPNTSKVK-RPVAKYHPSIWKDHFLSLSSEAMKIDVEMEERAKQLKAEV 63

Query: 65  RKMLFVVMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVV 124
           +KML +  RN LEKL LVD+IQRL + YHF N+IDQ+LE+IY  Y D       +DL++V
Sbjct: 64  KKMLMISNRNLLEKLSLVDSIQRLSVFYHFENEIDQILEKIYANYRDFTSIDGYDDLHIV 123

Query: 125 ALLFRLLRQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEA 184
           +LLFRLLRQ GY+I C                        LSLYEAA+MR+HGED+LEEA
Sbjct: 124 SLLFRLLRQHGYRIPCDIFTKFVESNGKFKESLAEDARGILSLYEAANMRVHGEDVLEEA 183

Query: 185 LEFTIKHLKNLIANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTF 244
           L+F++KHLKN+IANS+S    E++ ALK PLR  LP++KAREY+ +Y++DPSHS  LL F
Sbjct: 184 LDFSVKHLKNIIANSNSKLGAEIEHALKWPLRKDLPKLKAREYITMYQEDPSHSPTLLAF 243

Query: 245 SKLDFNILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVARE 304
           SKLDFNILQK + KEL  +TRWWK L++ TNFP+ARDRIVE YFW LGAYFEP FNV R+
Sbjct: 244 SKLDFNILQKLYQKELKELTRWWKDLNVETNFPYARDRIVECYFWTLGAYFEPQFNVGRK 303

Query: 305 ILTKVIGMISILDDIYDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFEE 364
           ILTKVI + SILDD YDAYGT             WD    ++LPEYMK  YI +L+ ++E
Sbjct: 304 ILTKVIAISSILDDTYDAYGTFEELQIFTEAIQRWDRSMVSILPEYMKPLYIAMLNHYKE 363

Query: 365 IGKKIDKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVA 424
           I K+I KD +  H++  KE +KKL +SY +EA+W ++ YKP FKEYME AL ++GY  + 
Sbjct: 364 ISKEIGKDENSLHVHTPKEEIKKLARSYFEEAKWLNENYKPKFKEYMEWALHSTGYFSII 423

Query: 425 TISFLGMGNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYME 484
           +++ +GMG+I+T ++ EW F  PKII AS +ICRLMDDIVS KFEQ+REHV   + CY E
Sbjct: 424 SVALIGMGDIVTNKILEWFFRKPKIINASTIICRLMDDIVSGKFEQEREHVTSGVECYKE 483

Query: 485 QYNCSEEEACVEFHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYT 527
           QY CSEEEAC+E HKQ+ DAWKDINEAC   + VP P+LMR++N+SRVINLLY +EDGYT
Sbjct: 484 QYGCSEEEACIELHKQVVDAWKDINEACLHPIDVPMPILMRIVNVSRVINLLYTDEDGYT 543

BLAST of Tan0010162 vs. TAIR 10
Match: AT5G23960.2 (terpene synthase 21 )

HSP 1 Score: 354.4 bits (908), Expect = 1.6e-97
Identity = 198/545 (36.33%), Postives = 308/545 (56.51%), Query Frame = 0

Query: 22  SRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVRKMLFVVMRNPLEKLKLVD 81
           +RP A F  +IW+D   S+S   L  E    E+    K  V++       NP+E +K +D
Sbjct: 6   NRPLADFPANIWEDPLTSFSKSDLGTE-TFKEKHSTLKEAVKEAFMSSKANPIENIKFID 65

Query: 82  TIQRLGISYHFGNDIDQVLEEIYMAYNDLLD-----KYNDEDLNVVALLFRLLRQQGYKI 141
            + RLG+SYHF  DI + L++ +    D LD     +    DL  V ++F++ RQ G+K+
Sbjct: 66  ALCRLGVSYHFEKDIVEQLDKSF----DCLDFPQMVRQEGCDLYTVGIIFQVFRQFGFKL 125

Query: 142 SC----------------------ACLSLYEAAHMRIHGEDLLEEALEFTIKHLKNLIAN 201
           S                         LSLYEAA    HGED+++EAL F+  HL+ + + 
Sbjct: 126 SADVFEKFKDENGKFKGHLVTDAYGMLSLYEAAQWGTHGEDIIDEALAFSRSHLEEISSR 185

Query: 202 SSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFSKLDFNILQKRHPK 261
           SS    I +++ALK P    + RI+ R+Y+  YE++ S    LL F+K+DFN+LQ  H +
Sbjct: 186 SSPHLAIRIKNALKHPYHKGISRIETRQYISYYEEEESCDPTLLEFAKIDFNLLQILHRE 245

Query: 262 ELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREILTKVIGMISILDD 321
           EL  +TRW   ++  +   + R RI E Y W LG YFEP ++ AR I T  + + + LDD
Sbjct: 246 ELACVTRWHHEMEFKSKVTYTRHRITEAYLWSLGTYFEPQYSQARVITTMALILFTALDD 305

Query: 322 IYDAYGTWD--------IDATNL-----LPEYMKFYYIVLLDLFEEIGKKIDKDGSLYHI 381
           +YDAYGT +        +D  ++     +P+ MKF Y V ++ ++++ ++++K+G     
Sbjct: 306 MYDAYGTMEELELFTDAMDEYSICNIIPIPDSMKFIYNVTVEFYDKLDEELEKEGRSGCG 365

Query: 382 YFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVATISFLGMGNIITKE 441
           +  K++++K    Y++EA+W  K Y   F EY E A+ +SGY  +  ++F+ M ++   +
Sbjct: 366 FHLKKSLQKTANGYMQEAKWLKKDYIATFDEYKENAILSSGYYALIAMTFVRMTDVAKLD 425

Query: 442 VFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQYNCSEEEACVEFH 501
            FEW+   PKI  AS++I R  DDI S++FE +REHV   I+CYM+Q+  S+E A     
Sbjct: 426 AFEWLSSHPKIRVASEIISRFTDDISSYEFEHKREHVATGIDCYMQQFGVSKERAVEVMG 485

Query: 502 KQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTHGKGQTKLFIKSLL 527
             + DAWKD+N+      + P P+LMRVLNLSRVI++ YR +D YT+ K   K  I SLL
Sbjct: 486 NIVSDAWKDLNQELMRPHVFPFPLLMRVLNLSRVIDVFYRYQDAYTNPK-LLKEHIVSLL 544

BLAST of Tan0010162 vs. TAIR 10
Match: AT5G23960.1 (terpene synthase 21 )

HSP 1 Score: 353.2 bits (905), Expect = 3.6e-97
Identity = 199/547 (36.38%), Postives = 307/547 (56.12%), Query Frame = 0

Query: 22  SRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVRKMLFVVMRNPLEKLKLVD 81
           +RP A F  +IW+D   S+S   L  E    E+    K  V++       NP+E +K +D
Sbjct: 6   NRPLADFPANIWEDPLTSFSKSDLGTE-TFKEKHSTLKEAVKEAFMSSKANPIENIKFID 65

Query: 82  TIQRLGISYHFGNDIDQVLEEIYMAYNDLLD-----KYNDEDLNVVALLFRLLRQQGYKI 141
            + RLG+SYHF  DI + L++ +    D LD     +    DL  V ++F++ RQ G+K+
Sbjct: 66  ALCRLGVSYHFEKDIVEQLDKSF----DCLDFPQMVRQEGCDLYTVGIIFQVFRQFGFKL 125

Query: 142 SC----------------------ACLSLYEAAHMRIHGEDLLEEALEFTIKHLKNLIAN 201
           S                         LSLYEAA    HGED+++EAL F+  HL+ + + 
Sbjct: 126 SADVFEKFKDENGKFKGHLVTDAYGMLSLYEAAQWGTHGEDIIDEALAFSRSHLEEISSR 185

Query: 202 SSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFSKLDFNILQKRHPK 261
           SS    I +++ALK P    + RI+ R+Y+  YE++ S    LL F+K+DFN+LQ  H +
Sbjct: 186 SSPHLAIRIKNALKHPYHKGISRIETRQYISYYEEEESCDPTLLEFAKIDFNLLQILHRE 245

Query: 262 ELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREILTKVIGMISILDD 321
           EL  +TRW   ++  +   + R RI E Y W LG YFEP ++ AR I T  + + + LDD
Sbjct: 246 ELACVTRWHHEMEFKSKVTYTRHRITEAYLWSLGTYFEPQYSQARVITTMALILFTALDD 305

Query: 322 IYDAYGTWD-----IDATN----------LLPEYMKFYYIVLLDLFEEIGKKIDKDGSLY 381
           +YDAYGT +      DA +           +P+ MKF Y V ++ ++++ ++++K+G   
Sbjct: 306 MYDAYGTMEELELFTDAMDEWLPVVPDEIPIPDSMKFIYNVTVEFYDKLDEELEKEGRSG 365

Query: 382 HIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVATISFLGMGNIIT 441
             +  K++++K    Y++EA+W  K Y   F EY E A+ +SGY  +  ++F+ M ++  
Sbjct: 366 CGFHLKKSLQKTANGYMQEAKWLKKDYIATFDEYKENAILSSGYYALIAMTFVRMTDVAK 425

Query: 442 KEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQYNCSEEEACVE 501
            + FEW+   PKI  AS++I R  DDI S++FE +REHV   I+CYM+Q+  S+E A   
Sbjct: 426 LDAFEWLSSHPKIRVASEIISRFTDDISSYEFEHKREHVATGIDCYMQQFGVSKERAVEV 485

Query: 502 FHKQIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTHGKGQTKLFIKS 527
               + DAWKD+N+      + P P+LMRVLNLSRVI++ YR +D YT+ K   K  I S
Sbjct: 486 MGNIVSDAWKDLNQELMRPHVFPFPLLMRVLNLSRVIDVFYRYQDAYTNPK-LLKEHIVS 545

BLAST of Tan0010162 vs. TAIR 10
Match: AT1G31950.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 327.4 bits (838), Expect = 2.1e-89
Identity = 184/559 (32.92%), Postives = 305/559 (54.56%), Query Frame = 0

Query: 6   FSVVSSSKSPDISHVKSRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVRKM 65
           F V SSSK        S PT  F PS+W DHFLS S    +  D++  ++E  K   R  
Sbjct: 48  FFVASSSKKSSDDLESSLPTPHFSPSLWGDHFLSVSLNRAVEFDELEREIETTKPLARDK 107

Query: 66  LFVVMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVVALL 125
           L     +  EK++L+  +  +GISYHF  +I  +L+  +   +D++    ++DL  ++++
Sbjct: 108 LMSSESSDKEKIRLIHLLVSMGISYHFDKEIQDILKHSFTKLDDII--VGEDDLETISIM 167

Query: 126 FRLLRQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEALEF 185
           F + +  G+K+SC                        L L+E AH+    E +++EAL F
Sbjct: 168 FEVFKLYGHKMSCDAFDRFRGNDGRFKESLVRDFRGMLQLFEVAHLGTPCEVIMDEALSF 227

Query: 186 TIKHLKNLIANSSS----WFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLT 245
           T  HL++L + ++S      +  +Q++L  P    +  + AREY+  YEQ+  H +ILL 
Sbjct: 228 TRNHLESLTSGNASTASPHLLKHIQNSLYIPRYCNIEVLVAREYISYYEQEEGHDEILLK 287

Query: 246 FSKLDFNILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAR 305
           F+KL+FN  Q  + +EL  +T+WW+ LD+ +  P+ RDR+VE +   LG YFEPH+++ R
Sbjct: 288 FAKLNFNFCQFHYVQELKTLTKWWRDLDLASKLPYIRDRLVESHLVALGPYFEPHYSLGR 347

Query: 306 EILTKVIGMISILDDIYDAYGT-----------WDIDATNLLPEYMKFYYIVLLDLFEEI 365
            I+ K+  ++ ++DD YDAY T             I+ ++ LP+Y++     L D+  EI
Sbjct: 348 IIVAKINMIMVVVDDTYDAYATLPQVKALTECLQSIEVSDKLPDYLRIVLGSLFDVMGEI 407

Query: 366 GKKIDKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVAT 425
            +++   G LY +    E +K + K+Y + A+W+   +   F EYM++ + T+G ++ A 
Sbjct: 408 EREMRPLGRLYRVKQVVEKIKIITKAYQEIAKWARTGHVSTFDEYMKVGVLTAGMADYAA 467

Query: 426 ISFLGMGNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQ 485
             F+GM +I  KE FEW+   P II+    + RL +D+ +++ E  R  V   +NCYM+Q
Sbjct: 468 YCFIGMEDINEKEAFEWLNSNPLIIKHLTAMFRLANDVGTYETEINRGEVANGLNCYMKQ 527

Query: 486 YNCSEEEACVEFHKQIGDAWKDINEACFD-RLIVPRPVLMRVLNLSRVINLLYREEDGYT 527
           Y  ++EEA  E  K      K + E   +    VPR VL+R LN++R+ ++ Y E DGY+
Sbjct: 528 YGVTKEEASRELRKMYVYRKKVVVEEFMNSHDHVPRQVLLRCLNIARIFDVFYTEGDGYS 587

BLAST of Tan0010162 vs. TAIR 10
Match: AT3G14490.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 321.6 bits (823), Expect = 1.2e-87
Identity = 188/545 (34.50%), Postives = 290/545 (53.21%), Query Frame = 0

Query: 22  SRPTAKFHPSIWKDHFLSYSSEALIIEDKMIEQVEQQKNEVRKMLFVVMRNPLEKLKLVD 81
           SRP A F P++W DHFLS        +D   E     K +VR ML     +  E+++L+ 
Sbjct: 59  SRPLAHFSPTLWGDHFLSVPLHVAEFDDFSREIEVTMKPKVRDMLKSSKNSDNERIRLIH 118

Query: 82  TIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVVALLFRLLRQQGYKISC--- 141
            +  LGI+YHF  +ID++L + +   +D++ K N  DL  ++ +F + R +GY + C   
Sbjct: 119 LLMNLGIAYHFEIEIDEILGQAFGNLDDIIAKEN--DLETISTMFEVFRLRGYYMPCYAF 178

Query: 142 -------------------ACLSLYEAAHMRIHGEDLLEEALEFTIKHLKNLIAN----S 201
                                L LYEAAH+    ED+++EAL FT   L++L +N    +
Sbjct: 179 NRFKGEDGRFKESLAEDIRGMLQLYEAAHLGTPSEDIMDEALSFTRYRLESLTSNHTATA 238

Query: 202 SSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFSKLDFNILQKRHPKE 261
           S      +Q+AL       L  + AREY+  YEQ+  H + LL F+KL+FN  Q  + +E
Sbjct: 239 SPHLSKHIQNALYRARYHNLEILVAREYISFYEQEEDHDETLLKFAKLNFNYCQLHYIQE 298

Query: 262 LGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREILTKVIGMISILDDI 321
           L  +T+WWK LD+ +  P+ RDRIVE+YF  L  YFEP +++ R I+TK+  ++++ +D 
Sbjct: 299 LKDLTKWWKELDLASKLPYIRDRIVEVYFGALALYFEPRYSLGRIIVTKITMIVTVFNDT 358

Query: 322 YDAYGT-------------WDIDATNLLPEYMKFYYIVLLDLFEEIGKKIDKDGSLYHIY 381
            DAYGT             WD+     LP Y+K  +  + +  EEI +++   G    + 
Sbjct: 359 CDAYGTLPEVTSLVDSFQRWDLGDIEKLPSYVKIVFRGVFETLEEIEQEMRPQGRSRIVQ 418

Query: 382 FAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEVATISFLGMGNIITKEV 441
            A + +KKL K+YL  ++W+   + P F+EYME  + TS     A  SF+ M +    + 
Sbjct: 419 VAVDEIKKLGKAYLAISKWARASHVPTFEEYMEFGMQTS-MDHFAAYSFIAMEDCDENQT 478

Query: 442 FEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYMEQYNCSEEEACVEFHK 501
            EW    PK++EA   + R+ +DI + + E  R  V   +NCYM+Q+  S+EEA  E +K
Sbjct: 479 CEWYKSRPKMMEALNGVFRIKNDINTFEQEMSRGEVAKGLNCYMKQHGVSKEEAIGEMNK 538

Query: 502 QIGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDGYTHGK-GQTKLFIKSLL 527
              + +K I E       VPRP+L+R LN+SR I+  Y+E D +T    G  K  I SL 
Sbjct: 539 IYSNYYKIIMEEYLTTTAVPRPILVRCLNVSRPIHHFYKERDEFTDPYFGMLKEVITSLF 598

BLAST of Tan0010162 vs. TAIR 10
Match: AT3G14540.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 2.2e-86
Identity = 190/561 (33.87%), Postives = 306/561 (54.55%), Query Frame = 0

Query: 9   VSSSKSPDISHVKSRPTAKFHPSIWKDHFLSYS-SEALIIEDKMIEQVEQQKNEVRKMLF 68
           V S+KS D     SRP+  F PS+W DHFLS S   A  +E  +  ++E  K  V+ ML 
Sbjct: 47  VRSTKSSDDLET-SRPSTYFSPSLWGDHFLSVSLDHAEFVE--LEREIETMKPLVKDMLM 106

Query: 69  VVMRNPLEKLKLVDTIQRLGISYHFGNDIDQVLEEIYMAYNDLLDKYNDEDLNVVALLFR 128
               +  EK++L+  +  LG SYHF  +I  +L+  +   + ++    ++DL  ++++F 
Sbjct: 107 SSQSSDKEKIRLIHLLVSLGSSYHFDKEIQDILKHSFTKLDGII--VEEDDLETISIMFE 166

Query: 129 LLRQQGYKISC----------------------ACLSLYEAAHMRIHGEDLLEEALEFTI 188
           + R  G+K+SC                        L L+E AH+    ED+++EAL FT 
Sbjct: 167 VFRLYGHKMSCDAFDRFRGGDGRFKESLAKDVRGMLQLFEVAHLGTLSEDIMDEALRFTR 226

Query: 189 KHLKNL----IANSSSWFMIEVQSALKCPLRLALPRIKAREYMLIYEQDPSHSDILLTFS 248
            HL++L    ++++S   +  +Q++L  P    +  + AREY+  YEQ+  +++ILL F+
Sbjct: 227 NHLESLTSGNVSSASPHILKHIQNSLYIPRYCNIEVLVAREYISYYEQEEGYNEILLKFA 286

Query: 249 KLDFNILQKRHPKELGAITRWWKALDIPTNFPFARDRIVELYFWVLGAYFEPHFNVAREI 308
           KL+FN  Q  + +E+  +T+WWK LD+ +  P+ RDR VE +   LG YFEP +++ R I
Sbjct: 287 KLNFNFCQCHYIQEIKTLTKWWKDLDLASKLPYIRDRSVESHLGGLGPYFEPQYSLGRII 346

Query: 309 LTKVIGMISILDDIYDAYGTWDIDATNLLPEYMK------------FYYIVLLDLFE--- 368
           + K I +I + DD YDA+ T  I    +L EY +            +  IVL  +FE   
Sbjct: 347 VAKTIMIIVVADDTYDAHAT--IPEATVLTEYFQRLNIGADDKLSGYLRIVLESVFEVMG 406

Query: 369 EIGKKIDKDGSLYHIYFAKEAVKKLVKSYLKEAEWSSKKYKPNFKEYMELALDTSGYSEV 428
           EI +++   G  Y +    E  K + K+Y +  EW+ K + P F EYM++ L T+G  + 
Sbjct: 407 EIEQEMSPKGRSYSVKQVLERFKIIAKAYKQLTEWARKGHVPTFDEYMKVGLVTAGMGDY 466

Query: 429 ATISFLGMGNIITKEVFEWVFDGPKIIEASKMICRLMDDIVSHKFEQQREHVVPAINCYM 488
           A   F+GM +I  KE FEW+   P +I+A  ++ R+ +D+ +++ E  R  V   +NCYM
Sbjct: 467 AGYCFIGMEDINEKEAFEWLNSNPLLIDALNVLFRIANDVGTYETEINRGEVANGLNCYM 526

Query: 489 EQYNCSEEEACVEFHKQ-IGDAWKDINEACFDRLIVPRPVLMRVLNLSRVINLLYREEDG 527
           +QY  ++EEA  E  K  I +    + E       VPR VL+R LN +R+ +++Y E DG
Sbjct: 527 KQYGVTKEEASRELRKMYIYNKKVVVEEFMNSHDRVPRQVLLRCLNFARLFDVIYTEGDG 586

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B2KSJ51.3e-17657.04(+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1[more]
Q6Q3H33.6e-15049.19(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S... [more]
B5A4357.7e-14548.10Sesquiterpene synthase OS=Santalum album OX=35974 PE=1 SV=1[more]
E3W2072.5e-14348.10Sesquiterpene synthase OS=Santalum austrocaledonicum OX=293154 PE=2 SV=1[more]
F6M8H53.9e-14146.88Probable sesquiterpene synthase OS=Santalum austrocaledonicum OX=293154 GN=Sesqu... [more]
Match NameE-valueIdentityDescription
XP_022142732.12.5e-20163.38(+)-gamma-cadinene synthase-like [Momordica charantia][more]
XP_023520451.11.5e-19060.93LOW QUALITY PROTEIN: (+)-gamma-cadinene synthase-like [Cucurbita pepo subsp. pep... [more]
XP_022932164.14.9e-18960.39(+)-gamma-cadinene synthase-like [Cucurbita moschata][more]
XP_023552098.14.6e-18760.33(+)-gamma-cadinene synthase-like [Cucurbita pepo subsp. pepo][more]
XP_022985144.15.6e-18559.60LOW QUALITY PROTEIN: (+)-gamma-cadinene synthase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1CNP61.2e-20163.38(+)-gamma-cadinene synthase-like OS=Momordica charantia OX=3673 GN=LOC111012779 ... [more]
A0A6J1EW932.4e-18960.39(+)-gamma-cadinene synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111438481 P... [more]
A0A6J1JCH12.7e-18559.60LOW QUALITY PROTEIN: (+)-gamma-cadinene synthase-like OS=Cucurbita maxima OX=366... [more]
A0A6J1EZA98.7e-18459.21LOW QUALITY PROTEIN: (+)-gamma-cadinene synthase-like OS=Cucurbita moschata OX=3... [more]
A0A6J1J7F93.7e-18257.78(+)-gamma-cadinene synthase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT5G23960.21.6e-9736.33terpene synthase 21 [more]
AT5G23960.13.6e-9736.38terpene synthase 21 [more]
AT1G31950.12.1e-8932.92Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT3G14490.11.2e-8734.50Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT3G14540.12.2e-8633.87Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008949Isoprenoid synthase domain superfamilyGENE3D1.10.600.10Farnesyl Diphosphate Synthasecoord: 228..527
e-value: 5.1E-113
score: 379.2
IPR008949Isoprenoid synthase domain superfamilyGENE3D1.10.600.10Farnesyl Diphosphate Synthasecoord: 30..70
e-value: 2.2E-30
score: 107.5
IPR008949Isoprenoid synthase domain superfamilySUPERFAMILY48576Terpenoid synthasescoord: 213..526
IPR036965Terpene synthase, N-terminal domain superfamilyGENE3D1.50.10.130coord: 141..227
e-value: 1.0E-23
score: 85.9
IPR036965Terpene synthase, N-terminal domain superfamilyGENE3D1.50.10.130coord: 71..140
e-value: 2.2E-30
score: 107.5
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 218..470
e-value: 1.8E-88
score: 296.3
IPR001906Terpene synthase, N-terminal domainPFAMPF01397Terpene_synthcoord: 32..139
e-value: 1.1E-28
score: 100.3
NoneNo IPR availableSFLDSFLDS00005Isoprenoid_Synthase_Type_Icoord: 216..525
e-value: 0.0
score: 306.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..19
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR31225:SF134(-)-GERMACRENE D SYNTHASE-LIKEcoord: 22..512
NoneNo IPR availablePANTHERPTHR31225OS04G0344100 PROTEIN-RELATEDcoord: 22..512
IPR034741Terpene cyclase-like 1, C-terminal domainSFLDSFLDG01019Terpene_Cyclase_Like_1_C_Termcoord: 216..525
e-value: 0.0
score: 306.0
IPR044814Terpene cyclases, class 1, plantCDDcd00684Terpene_cyclase_plant_C1coord: 23..524
e-value: 0.0
score: 589.165
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 29..213

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0010162.1Tan0010162.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016102 diterpenoid biosynthetic process
biological_process GO:0045338 farnesyl diphosphate metabolic process
biological_process GO:0051762 sesquiterpene biosynthetic process
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0010334 sesquiterpene synthase activity
molecular_function GO:0016829 lyase activity
molecular_function GO:0010333 terpene synthase activity