Tan0010121 (gene) Snake gourd v1

BLAST of Tan0010121 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 3.9e-162
Identity = 302/490 (61.63%), Postives = 371/490 (75.71%), Query Frame = 0

Query: 19  SLFPIDEATVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVLKSVLEVNPDARAQAEAAD 78
           S F I EAT+ +I++AF++ +LTS+QL++ YL  I  LNP+L +V+E NPDA  QAE AD
Sbjct: 36  STFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAISKLNPILHAVIETNPDALIQAEIAD 95

Query: 79  RERELAGGKALGELHGIPVLLKDSIATKDRLNTTAGSFALLGSVVPRDATVVDRLRNDGA 138
           RER+L     L  LHG+PVLLKDSI+TKD+LNTTAGSFALLGSVV RDA VV RLR  GA
Sbjct: 96  RERDLKNTTKLPILHGVPVLLKDSISTKDKLNTTAGSFALLGSVVARDAGVVKRLRESGA 155

Query: 139 VILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSL 198
           VILGK SL+EW   RS  IP GW ARG Q  NPY    +P GSSSGSAISV AN+ AVSL
Sbjct: 156 VILGKASLSEWAHFRSFSIPDGWSARGLQGKNPYVLSANPSGSSSGSAISVTANLVAVSL 215

Query: 199 GTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIV 258
           GTETDGSIL PA  NSVVGIKP+VGLTSRAGV+PIS RQD+IGPICRTVSDAV++LDAIV
Sbjct: 216 GTETDGSILSPASQNSVVGIKPSVGLTSRAGVVPISLRQDSIGPICRTVSDAVHLLDAIV 275

Query: 259 GFDPMDSEATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRHPFSLLYPNESMAVSTFDHH 318
           G+DP+D EATK AS FIP GGYKQFL   GL GKRLGIV    SLL           DHH
Sbjct: 276 GYDPLD-EATKTASEFIPEGGYKQFLTTSGLKGKRLGIVMKHSSLL-----------DHH 335

Query: 319 VNFVLRKSGATIVDNLQISNVDVILNTFQSGELVAMVAEFKIAINDYLKKLIHSPVRSLA 378
           +   LR+ GA +++NL I N++VI+    SGE +A++AEFK+++N YLK+L+ SPVRSLA
Sbjct: 336 IK-TLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAEFKMSLNAYLKELVKSPVRSLA 395

Query: 379 DIIAFNNNHPELENMKEYGQDAFLLSEQTDGIGEMEKEAISTMANLSRYGFEEMMKTYDL 438
           D+IA+N    E E +KE+GQ+ FL +E T G+GE EK A+  M  LSR G E++++   L
Sbjct: 396 DVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTALQKMKELSRNGIEKLIEENKL 455

Query: 439 DAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFGGLKGTEPKLIEIAYAYEQAT 498
           DA+VTLG+   +VLAIGGYP I+VPAGY+  G P+GI FGGL+ +EPKLIEIA+A+EQAT
Sbjct: 456 DAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISFGGLRFSEPKLIEIAFAFEQAT 512

Query: 499 RVRRPPPLLS 509
            +R+PP  ++
Sbjct: 516 LIRKPPKFIA 512

BLAST of Tan0010121 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 5.5e-79
Identity = 202/523 (38.62%), Postives = 279/523 (53.35%), Query Frame = 0

Query: 12  LFAGAHCSLFPIDEATVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVLKSVLEVNPDAR 71
           +F    C    +++AT+ ++Q       LTS  ++  YL++   +NP +  +L++NPD  
Sbjct: 55  IFPMPKCQNITLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVL 114

Query: 72  AQAEAADRERELAGGKALGELHGIPVLLKDSIATKDRLNTTAGSFALLGSVVPRDATVVD 131
             A   D ER  A G   G LHGIP ++KD+ ATKD+++TTAGS+ALLGS+VPRDA VV 
Sbjct: 115 TIASELDDER--ANGIIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVK 174

Query: 132 RLRNDGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAA 191
           +LR  GAV+ G  +L+EW   RS     G+ ARGGQ+  P+    +P GSSSGSAISVA+
Sbjct: 175 QLREAGAVLFGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVAS 234

Query: 192 NMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAV 251
           NM A +LGTETDGSI+ PA  N VVG+KPTVGLTSR GVIP S  QDT GPI RTV DAV
Sbjct: 235 NMIAFALGTETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAV 294

Query: 252 YVLDAIVGFDPMDSEATKLASAFIPSGGYKQFL-NKDGLLGKRLGIVRHPFSLLYPNESM 311
           YV  ++ G D  D             G Y +FL NK  L G R G+   P+  L+ N   
Sbjct: 295 YVFQSMWGIDENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGL---PWKRLWQNAK- 354

Query: 312 AVSTFDHHVNFV--LRKSGATIVDNLQISNVDVI--------LNTFQSGELVAMVAEFKI 371
                D  +  V  + ++GA + +N    N+DVI        L +    E   +  +F  
Sbjct: 355 -ADEIDRLLEVVKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYN 414

Query: 372 AINDYLKKLIHSPVRSLADIIAFNNNHPELENMK-------EYGQDAFLLSEQTDGI-GE 431
            I  YL ++ ++ + SL DI+ +NN +   E  K         GQD FL S +  G+  E
Sbjct: 415 NIKSYLSEVKNTEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNE 474

Query: 432 MEKEAISTMANLSR-YGFEEMM-----KTYDL----DAMVTLGTG-AETVLAIGGYPAIS 491
              +A+  +   S+  G +  +     KT D       +V  GT       A  GYP I+
Sbjct: 475 TYWQAVEYVRRTSQDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMIT 534

Query: 492 VPAGYEGNGEPFGILFGGLKGTEPKLIEIAYAYEQATRVRRPP 505
           +P G + NG PFG+        EP+LI+   A E   + +  P
Sbjct: 535 LPIGVKTNGRPFGLGIMHSAWQEPQLIKYGSAIEDLLQYKAKP 570

BLAST of Tan0010121 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 6.5e-64
Identity = 167/492 (33.94%), Postives = 264/492 (53.66%), Query Frame = 0

Query: 31  IQLAFSQNKLTSRQLLDHYLNKIDALNPVLKSVLEVNPDARAQAEAADRERELAGGKALG 90
           +Q  + Q  +    ++  Y+ +I  +N  +++V E+NPDA   A+  D ER++  GK  G
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKM--GKLRG 153

Query: 91  ELHGIPVLLKDSIATKDRLNTTAGSFALLGSVVPRDATVVDRLRNDGAVILGKTSLTEWY 150
            LHG+P+++K++I T D++++TAGS+A+ G+    DATV  +LR  G VI+GK+  ++W 
Sbjct: 154 PLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQWA 213

Query: 151 GSRSLKIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGSILCPA 210
             RSL   +GW A GGQ    Y K  DP GSSSGS ++    +A  +LGTET GSI+ PA
Sbjct: 214 NFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSPA 273

Query: 211 DYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDSEATKL 270
           D +++VG+KPTVGLTSR  V+PIS RQDT+GP+ R+V DA Y+L  I G D  D+  + +
Sbjct: 274 DKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYTSAI 333

Query: 271 ASAFIPSGGYKQFLNKDGLLGKRLGIVRHPFSLLYPNESMAVSTFDHHVNFVLRKSGATI 330
               IP   Y +  + + L GKR+G+ R+    ++ +    V  F+  +  V++K+GA I
Sbjct: 334 PFDTIPD--YVKACDINALKGKRIGVPRNVIK-IFGSPQTVVDQFNQAL-AVMKKAGAII 393

Query: 331 VDNLQISNVDVILNTFQSGELVAMVAEFKIAINDYLKKLIHSP--VRSLADIIAFNNNHP 390
           V+N   ++      +    ++  + A+    +  + K+L  +P  +  L  +  F  +H 
Sbjct: 394 VENTDFTSFAEFAQSPIPDDI--LYADSLTNLPAFFKQLKVNPHNITDLESLRRFTQHH- 453

Query: 391 ELENMKEYGQDAFLLSEQTDGIGEMEKEAISTMANLSRYGFE----EMMKTYDLDAMVTL 450
            LE         + ++ Q  GI   + +         ++G E      ++ + LDA V  
Sbjct: 454 RLEEYPSRDTARWDIALQ-KGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLP 513

Query: 451 GTGAETVLAIGGYPAISVPAGY---------------EGNGEPFGILFGGLKGTEPKLIE 502
              +  + A+ G P I+VP G                 G G P GI F G   +E KLI 
Sbjct: 514 TDLSPYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIG 573

BLAST of Tan0010121 vs. ExPASy Swiss-Prot
Match: Q0I9N6 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Synechococcus sp. (strain CC9311) OX=64471 GN=gatA PE=3 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 8.8e-45
Identity = 159/506 (31.42%), Postives = 234/506 (46.25%), Query Frame = 0

Query: 28  VAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVLKSVLEVNPD-ARAQAEAADRERELAGG 87
           +AE +      ++++R+L DH+L +I A++  + + LEV  D ARA A+  D  R  A G
Sbjct: 3   IAEWRQQLESGEVSARELTDHHLARIKAVDSSVHAFLEVTADRARADADRLDEAR--AAG 62

Query: 88  KALGELHGIPVLLKDSIATKDRLNTTAGSFALLGSVVPRDATVVDRLRNDGAVILGKTSL 147
           + L  L G+P+ +KD++ TK  + TT+ S  L   V P ++TV DRL   GAV++GKT+L
Sbjct: 63  EDLPPLAGVPIAIKDNLCTKG-IRTTSSSRMLESFVPPYESTVTDRLWRSGAVLIGKTNL 122

Query: 148 TEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGSI 207
            E+    S +      +  G   NP+  G  P GSS GSA +VAA     SLG++T GSI
Sbjct: 123 DEFAMGGSTE-----TSAFGPTANPWNTGYVPGGSSGGSAAAVAAGECMASLGSDTGGSI 182

Query: 208 LCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDSE 267
             PA +  VVG+KPT G  SR G++  +   D +GP   +VSDA  +L AI G DP DS 
Sbjct: 183 RQPASFCGVVGLKPTYGRVSRYGLVAFASSLDQVGPFATSVSDAAELLQAIAGEDPRDST 242

Query: 268 ATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRHPFSLLYPNESMAVSTFDHHVNFVLRKS 327
             K      P   Y++ L +  + G R+G+VR  F     +  +  S            +
Sbjct: 243 CLK-----APVPNYREVLGR-SVSGLRIGVVRECFDQEGIDPQVKASVL----------A 302

Query: 328 GATIVDNLQISNVDVILNTFQSGELVAMV-----AEFKIAINDYLKKLIHS-PVRSLADI 387
            A ++ +L    VDV    F  G     V     A   +A  D +K    +    SLA +
Sbjct: 303 AAELLQSLGAELVDVSCPRFNDGIATYYVIAPSEASANLARYDGVKYGFRAEDASSLASM 362

Query: 388 IAFNNNHPELENMKEYGQDAFLLSEQTDGIGEMEK--EAISTMANLSRYGFEEMMKTYDL 447
            A        E      Q   L+       G ++        +  L R  FE    + D+
Sbjct: 363 TA----RSRTEGFGSEVQRRILIGTYALSAGYVDAYYRKAQQVRTLIRRDFETAFASVDV 422

Query: 448 -------DAMVTLGTGAETVLA------------IGGYPAISVPAGYEGNGEPFGILFGG 504
                        G  A+  LA            + G PAI+VP G++  G P G+   G
Sbjct: 423 LLTPTAPSTAFAAGAHADDPLAMYLADLLTIPANLAGLPAINVPCGFDSEGLPIGVQLIG 480

BLAST of Tan0010121 vs. ExPASy Swiss-Prot
Match: B0JSX3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=gatA PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.7e-43
Identity = 158/505 (31.29%), Postives = 239/505 (47.33%), Query Frame = 0

Query: 27  TVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVLKSVLEVNPD-ARAQAEAADRERELAG 86
           ++ ++       + T+ ++   +L +I AL P +KS L + PD A AQA+  D   ++A 
Sbjct: 3   SIRQLHQQLVNKEKTAVEIATEFLARIQALEPQVKSFLHLTPDLALAQAQKVD--EKIAR 62

Query: 87  GKALGELHGIPVLLKDSIATKDRLNTTAGSFALLGSVVPRDATVVDRLRNDGAVILGKTS 146
           G++L  L GIP+ LKD++ TK  + TT  S  L   V P ++TV  +LR+ GAVI+GKT+
Sbjct: 63  GESLHLLAGIPIALKDNLCTKG-IPTTCASRILENFVPPYESTVTQKLRDLGAVIVGKTN 122

Query: 147 LTEW-YGSRSLKIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDG 206
           L E+  GS +    +   A      NP+     P GSS GSA +VAA    V+LG++T G
Sbjct: 123 LDEFAMGSSTENSGYHVTA------NPWDLSRVPGGSSGGSAAAVAAQECVVALGSDTGG 182

Query: 207 SILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMD 266
           SI  PA +  VVG+KPT GL SR G++  +   D IGP  RTV DA  +L AI G+DP D
Sbjct: 183 SIRQPASFCGVVGLKPTYGLVSRFGLVAYASSLDQIGPFGRTVEDAAILLQAIAGYDPQD 242

Query: 267 SEATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRHPFSLLYPNESMAVSTFDHHVNFVLR 326
           S +  L     P   Y QFL K  L G ++G+++  F          V+   +     L+
Sbjct: 243 STSLNL-----PIPDYSQFL-KTSLKGLKIGVIKETFG---EGLDQVVAEAVNQALAQLK 302

Query: 327 KSGATIVDNLQISNVDVILNTFQSGELVAMVAEFKIAINDYLKKLIHSPVRSLADIIAFN 386
             GATI + +        L T+    +    A   +A  D +K  I     SL D+    
Sbjct: 303 ALGATIKE-ISCPRFRYGLPTYYI--IAPSEASANLARYDGVKYGIREDADSLIDMYT-- 362

Query: 387 NNHPELENMKEYGQDA---FLLSEQTDGIGEMEKEAIST--MANLSRYGFEEMMKTYDLD 446
                    K +G +     +L   T   G  +   +    +  L +  F+   ++ D+ 
Sbjct: 363 -----KTRAKGFGAEVKRRIMLGTYTLSAGYYDAYYLKAQKVRTLIKEDFDRAFQSVDVL 422

Query: 447 AMVTLGT-----GAETV--------------LAIGGYPAISVPAGYEGNGEPFGILFGGL 506
              T  T     G +T               + + G P +S+P G++G G P G+   G 
Sbjct: 423 VSPTSPTTAFKAGEKTADPLSMYLSDLMTIPVNLAGLPGLSLPCGFDGQGLPIGLQLVGN 479

BLAST of Tan0010121 vs. NCBI nr
Match: XP_022989045.1 (putative amidase C869.01 [Cucurbita maxima] >XP_022989046.1 putative amidase C869.01 [Cucurbita maxima])

HSP 1 Score: 894.8 bits (2311), Expect = 3.5e-256
Identity = 460/513 (89.67%), Postives = 478/513 (93.18%), Query Frame = 0

Query: 1   MRVVFLLSAFILFAGAHCSLFPIDEATVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVL 60
           M +VF LSAF+ F GA+ S FPIDEATVAEIQ AFSQNKLTSR+LLDHYLNKID LNPVL
Sbjct: 1   MNIVFFLSAFLFFIGANSSQFPIDEATVAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVL 60

Query: 61  KSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIATKDRLNTTAGSFALLG 120
           KSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSI TKDRLNTTAGSFALLG
Sbjct: 61  KSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLG 120

Query: 121 SVVPRDATVVDRLRNDGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCG 180
           SVVPRDA VV RLRN GAVILGKTSLTEWYGSRS+KIPHGWCARGGQALNPYGKGGDPCG
Sbjct: 121 SVVPRDAKVVHRLRNAGAVILGKTSLTEWYGSRSMKIPHGWCARGGQALNPYGKGGDPCG 180

Query: 181 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 240
           SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI
Sbjct: 181 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 240

Query: 241 GPICRTVSDAVYVLDAIVGFDPMDSEATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRHP 300
           GPICRTVSDAVYVLDAIVGFDPMDSEATK+ S FIPSGGYKQFL +DGL GKRLGIVRHP
Sbjct: 241 GPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHP 300

Query: 301 FSLLYPNESMAVSTFDHHVNFVLRKSGATIVDNLQISNVDVILNTFQSGELVAMVAEFKI 360
           FS LY NES A+ +F+HHV  +LRKSGATIVDNLQISNV  ILN ++SGELVAM+AEFK+
Sbjct: 301 FSDLYANESTAIQSFEHHVK-LLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKL 360

Query: 361 AINDYLKKLIHSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTDGIGEMEKEAIST 420
            INDYLK LI SPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQT+GIGE EK AIS 
Sbjct: 361 TINDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISM 420

Query: 421 MANLSRYGFEEMMKTYDLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFGGL 480
           M NLSR+GFEEMMKTY+LDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILF GL
Sbjct: 421 MENLSRHGFEEMMKTYNLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFSGL 480

Query: 481 KGTEPKLIEIAYAYEQATRVRRPPPLLSKLSSL 514
           KGTEPKLIEIAYAYEQAT VRRPPPLLS LSSL
Sbjct: 481 KGTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 512

BLAST of Tan0010121 vs. NCBI nr
Match: XP_022928333.1 (putative amidase C869.01 [Cucurbita moschata])

HSP 1 Score: 892.5 bits (2305), Expect = 1.7e-255
Identity = 458/513 (89.28%), Postives = 477/513 (92.98%), Query Frame = 0

Query: 1   MRVVFLLSAFILFAGAHCSLFPIDEATVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVL 60
           M +VF LS F+LF GA+ S FPIDEAT+AEIQ AFSQNKLTSR+LLDHYLNKID LNPVL
Sbjct: 36  MNIVFFLSVFLLFVGANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVL 95

Query: 61  KSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIATKDRLNTTAGSFALLG 120
           +SVLEVNPDARAQAE ADRERELAGGKALGELHGIPVLLKDSI TKDRLNTTAGSFALLG
Sbjct: 96  RSVLEVNPDARAQAETADRERELAGGKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLG 155

Query: 121 SVVPRDATVVDRLRNDGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCG 180
           SVVPRDATVV RLRN GAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCG
Sbjct: 156 SVVPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCG 215

Query: 181 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 240
           SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI
Sbjct: 216 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 275

Query: 241 GPICRTVSDAVYVLDAIVGFDPMDSEATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRHP 300
           GPICRTVSDAVYVLDAIVGFDPMDSEATK+ S FIPSGGYKQFL +DGL GKRLGIVRHP
Sbjct: 276 GPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHP 335

Query: 301 FSLLYPNESMAVSTFDHHVNFVLRKSGATIVDNLQISNVDVILNTFQSGELVAMVAEFKI 360
           FS LY N S A+ TF+HHV  +LRKSGATIVDNLQISNV  ILN ++SGELVAM+AEFK+
Sbjct: 336 FSDLYANGSKAIQTFEHHVK-LLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKL 395

Query: 361 AINDYLKKLIHSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTDGIGEMEKEAIST 420
            INDYLK LI SPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQT+GIGE EK AIS 
Sbjct: 396 TINDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISM 455

Query: 421 MANLSRYGFEEMMKTYDLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFGGL 480
           M+NLSR GFEEMMKTY+LDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFG+LF GL
Sbjct: 456 MSNLSRDGFEEMMKTYNLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGL 515

Query: 481 KGTEPKLIEIAYAYEQATRVRRPPPLLSKLSSL 514
           KGTEPKLIEIAYAYEQAT VRRPPPLLS LSSL
Sbjct: 516 KGTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 547

BLAST of Tan0010121 vs. NCBI nr
Match: XP_023529660.1 (probable amidase At4g34880 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 891.7 bits (2303), Expect = 2.9e-255
Identity = 458/513 (89.28%), Postives = 479/513 (93.37%), Query Frame = 0

Query: 1   MRVVFLLSAFILFAGAHCSLFPIDEATVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVL 60
           M +VF LSAF+LF GA+ S FPIDEAT+AEIQ AFSQNKLTSR+LLDHYLNKID LNPVL
Sbjct: 43  MNIVFFLSAFLLFIGANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVL 102

Query: 61  KSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIATKDRLNTTAGSFALLG 120
           +SVLEVNPDARAQAEAADRER+LAGGKALGELHGIPVLLKDSI TKDRLNTTAGSFALLG
Sbjct: 103 RSVLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLG 162

Query: 121 SVVPRDATVVDRLRNDGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCG 180
           SVVPRDATVV RLRN GAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCG
Sbjct: 163 SVVPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCG 222

Query: 181 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 240
           SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI
Sbjct: 223 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 282

Query: 241 GPICRTVSDAVYVLDAIVGFDPMDSEATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRHP 300
           GPICRTVSDAVYVLDAIVGFDPMDSEATK+ S FIPSGGYKQFL +DGL GKRLGIVRHP
Sbjct: 283 GPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHP 342

Query: 301 FSLLYPNESMAVSTFDHHVNFVLRKSGATIVDNLQISNVDVILNTFQSGELVAMVAEFKI 360
           FS LY N S A+ +F+HHV  +LRKSGATIVDNLQISNV VI N ++SGELVAM+AEFK+
Sbjct: 343 FSDLYANGSTAIQSFEHHVK-LLRKSGATIVDNLQISNVAVIQNPYESGELVAMIAEFKL 402

Query: 361 AINDYLKKLIHSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTDGIGEMEKEAIST 420
            INDYLK LI SPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQT+GIGE EK AIS 
Sbjct: 403 TINDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISM 462

Query: 421 MANLSRYGFEEMMKTYDLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFGGL 480
           M+NLSR GFEEMMKTY+LDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFG+LF GL
Sbjct: 463 MSNLSRDGFEEMMKTYNLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGL 522

Query: 481 KGTEPKLIEIAYAYEQATRVRRPPPLLSKLSSL 514
           KGTEPKLIEIAYAYEQAT VRRPPPLLS LSSL
Sbjct: 523 KGTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 554

BLAST of Tan0010121 vs. NCBI nr
Match: KAG7015266.1 (hypothetical protein SDJN02_22900 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 891.0 bits (2301), Expect = 5.0e-255
Identity = 456/513 (88.89%), Postives = 478/513 (93.18%), Query Frame = 0

Query: 1   MRVVFLLSAFILFAGAHCSLFPIDEATVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVL 60
           M ++F LS F+LF GA+ S FPIDEAT+AEIQ AFSQNKLTSR+LLDHYLNKID LNPVL
Sbjct: 36  MNIIFFLSVFLLFVGANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVL 95

Query: 61  KSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIATKDRLNTTAGSFALLG 120
           +SVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSI TKDRLNTTAGSFALLG
Sbjct: 96  RSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLG 155

Query: 121 SVVPRDATVVDRLRNDGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCG 180
           SVVPRDATVV RLRN GAVILGKTSLTEWYGSRSL+IPHGWCARGGQALNPYGKGGDPCG
Sbjct: 156 SVVPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCG 215

Query: 181 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 240
           SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI
Sbjct: 216 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 275

Query: 241 GPICRTVSDAVYVLDAIVGFDPMDSEATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRHP 300
           GPICRTVSDAVYVLDAIVGFDPMDSEATK+ S FIPSGGYKQFL +DGL GKRLGIVRHP
Sbjct: 276 GPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHP 335

Query: 301 FSLLYPNESMAVSTFDHHVNFVLRKSGATIVDNLQISNVDVILNTFQSGELVAMVAEFKI 360
           FS LY N S A+ TF+HHV  +LRKSGATIVDNLQISNV  ILN ++SGELVAM+AEFK+
Sbjct: 336 FSDLYANGSKAIQTFEHHVK-LLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKL 395

Query: 361 AINDYLKKLIHSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTDGIGEMEKEAIST 420
            INDYLK LI SPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQT+GIGE EK AIS 
Sbjct: 396 TINDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISM 455

Query: 421 MANLSRYGFEEMMKTYDLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFGGL 480
           M+NLSR GFEEMMKTY+LD+MVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFG+LF GL
Sbjct: 456 MSNLSRDGFEEMMKTYNLDSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGL 515

Query: 481 KGTEPKLIEIAYAYEQATRVRRPPPLLSKLSSL 514
           KGTEPKLIEIAYAYEQAT VRRPPPLLS LSSL
Sbjct: 516 KGTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 547

BLAST of Tan0010121 vs. NCBI nr
Match: KAG6589001.1 (putative amidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 889.4 bits (2297), Expect = 1.5e-254
Identity = 455/513 (88.69%), Postives = 477/513 (92.98%), Query Frame = 0

Query: 1   MRVVFLLSAFILFAGAHCSLFPIDEATVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVL 60
           M ++F LS F+LF GA+ S FPIDEAT+AEIQ AFSQNKLTSR+LLDHYLNKID LNPVL
Sbjct: 36  MNIIFFLSVFLLFVGANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVL 95

Query: 61  KSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIATKDRLNTTAGSFALLG 120
           +SVLEVNPDARAQAEAADRERELAGGK LGELHGIPVLLKDSI TKDRLNTTAGSFALLG
Sbjct: 96  RSVLEVNPDARAQAEAADRERELAGGKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLG 155

Query: 121 SVVPRDATVVDRLRNDGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCG 180
           SVVPRDATVV RLRN GAVILGKTSLTEWYGSRSL+IPHGWCARGGQALNPYGKGGDPCG
Sbjct: 156 SVVPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCG 215

Query: 181 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 240
           SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI
Sbjct: 216 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 275

Query: 241 GPICRTVSDAVYVLDAIVGFDPMDSEATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRHP 300
           GPICRTVSDAVYVLDAIVGFDPMDSEATK+ S FIPSGGYKQFL +DGL GKRLGIVRHP
Sbjct: 276 GPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHP 335

Query: 301 FSLLYPNESMAVSTFDHHVNFVLRKSGATIVDNLQISNVDVILNTFQSGELVAMVAEFKI 360
           FS LY N S A+ TF+HHV  +LRKSGATIVDNLQISNV  ILN ++SGELVAM+AEFK+
Sbjct: 336 FSDLYANGSKAIQTFEHHVK-LLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKL 395

Query: 361 AINDYLKKLIHSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTDGIGEMEKEAIST 420
            INDYLK LI SPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQT+GIGE EK AIS 
Sbjct: 396 TINDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISM 455

Query: 421 MANLSRYGFEEMMKTYDLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFGGL 480
           M+NLSR GFEEMMKTY+LD+MVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFG+LF GL
Sbjct: 456 MSNLSRDGFEEMMKTYNLDSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGL 515

Query: 481 KGTEPKLIEIAYAYEQATRVRRPPPLLSKLSSL 514
           KGTEPKLIEIAYAYEQAT VRRPPPLLS LSSL
Sbjct: 516 KGTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 547

BLAST of Tan0010121 vs. ExPASy TrEMBL
Match: A0A6J1JLA2 (putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111486234 PE=4 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 1.7e-256
Identity = 460/513 (89.67%), Postives = 478/513 (93.18%), Query Frame = 0

Query: 1   MRVVFLLSAFILFAGAHCSLFPIDEATVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVL 60
           M +VF LSAF+ F GA+ S FPIDEATVAEIQ AFSQNKLTSR+LLDHYLNKID LNPVL
Sbjct: 1   MNIVFFLSAFLFFIGANSSQFPIDEATVAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVL 60

Query: 61  KSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIATKDRLNTTAGSFALLG 120
           KSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSI TKDRLNTTAGSFALLG
Sbjct: 61  KSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLG 120

Query: 121 SVVPRDATVVDRLRNDGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCG 180
           SVVPRDA VV RLRN GAVILGKTSLTEWYGSRS+KIPHGWCARGGQALNPYGKGGDPCG
Sbjct: 121 SVVPRDAKVVHRLRNAGAVILGKTSLTEWYGSRSMKIPHGWCARGGQALNPYGKGGDPCG 180

Query: 181 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 240
           SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI
Sbjct: 181 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 240

Query: 241 GPICRTVSDAVYVLDAIVGFDPMDSEATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRHP 300
           GPICRTVSDAVYVLDAIVGFDPMDSEATK+ S FIPSGGYKQFL +DGL GKRLGIVRHP
Sbjct: 241 GPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHP 300

Query: 301 FSLLYPNESMAVSTFDHHVNFVLRKSGATIVDNLQISNVDVILNTFQSGELVAMVAEFKI 360
           FS LY NES A+ +F+HHV  +LRKSGATIVDNLQISNV  ILN ++SGELVAM+AEFK+
Sbjct: 301 FSDLYANESTAIQSFEHHVK-LLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKL 360

Query: 361 AINDYLKKLIHSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTDGIGEMEKEAIST 420
            INDYLK LI SPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQT+GIGE EK AIS 
Sbjct: 361 TINDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISM 420

Query: 421 MANLSRYGFEEMMKTYDLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFGGL 480
           M NLSR+GFEEMMKTY+LDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILF GL
Sbjct: 421 MENLSRHGFEEMMKTYNLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFSGL 480

Query: 481 KGTEPKLIEIAYAYEQATRVRRPPPLLSKLSSL 514
           KGTEPKLIEIAYAYEQAT VRRPPPLLS LSSL
Sbjct: 481 KGTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 512

BLAST of Tan0010121 vs. ExPASy TrEMBL
Match: A0A6J1ENQ3 (putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111435192 PE=4 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 8.3e-256
Identity = 458/513 (89.28%), Postives = 477/513 (92.98%), Query Frame = 0

Query: 1   MRVVFLLSAFILFAGAHCSLFPIDEATVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVL 60
           M +VF LS F+LF GA+ S FPIDEAT+AEIQ AFSQNKLTSR+LLDHYLNKID LNPVL
Sbjct: 36  MNIVFFLSVFLLFVGANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVL 95

Query: 61  KSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIATKDRLNTTAGSFALLG 120
           +SVLEVNPDARAQAE ADRERELAGGKALGELHGIPVLLKDSI TKDRLNTTAGSFALLG
Sbjct: 96  RSVLEVNPDARAQAETADRERELAGGKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLG 155

Query: 121 SVVPRDATVVDRLRNDGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCG 180
           SVVPRDATVV RLRN GAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCG
Sbjct: 156 SVVPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCG 215

Query: 181 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 240
           SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI
Sbjct: 216 SSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTI 275

Query: 241 GPICRTVSDAVYVLDAIVGFDPMDSEATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRHP 300
           GPICRTVSDAVYVLDAIVGFDPMDSEATK+ S FIPSGGYKQFL +DGL GKRLGIVRHP
Sbjct: 276 GPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHP 335

Query: 301 FSLLYPNESMAVSTFDHHVNFVLRKSGATIVDNLQISNVDVILNTFQSGELVAMVAEFKI 360
           FS LY N S A+ TF+HHV  +LRKSGATIVDNLQISNV  ILN ++SGELVAM+AEFK+
Sbjct: 336 FSDLYANGSKAIQTFEHHVK-LLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKL 395

Query: 361 AINDYLKKLIHSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTDGIGEMEKEAIST 420
            INDYLK LI SPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQT+GIGE EK AIS 
Sbjct: 396 TINDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISM 455

Query: 421 MANLSRYGFEEMMKTYDLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFGGL 480
           M+NLSR GFEEMMKTY+LDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFG+LF GL
Sbjct: 456 MSNLSRDGFEEMMKTYNLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGL 515

Query: 481 KGTEPKLIEIAYAYEQATRVRRPPPLLSKLSSL 514
           KGTEPKLIEIAYAYEQAT VRRPPPLLS LSSL
Sbjct: 516 KGTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 547

BLAST of Tan0010121 vs. ExPASy TrEMBL
Match: A0A6J1C363 (putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111008028 PE=4 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 3.1e-242
Identity = 434/504 (86.11%), Postives = 463/504 (91.87%), Query Frame = 0

Query: 3   VVFLLSAFILFAGAHCSLFPIDEATVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVLKS 62
           +VF L+AF LF  ++ SLFPIDEAT+A+I  AFSQN LTSRQLLDHYLNKID LNPVL+S
Sbjct: 2   LVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRS 61

Query: 63  VLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIATKDRLNTTAGSFALLGSV 122
           VLEVNPDARAQAEAADRER+LAGGKALGELHGIPVLLKDSIATK  LNTTAGSFALLGSV
Sbjct: 62  VLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSV 121

Query: 123 VPRDATVVDRLRNDGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCGSS 182
           VPRDATVV RLR  GAVILGK SLTEWYGSRSLKIP+GWCARGGQALNPYGK GDPCGSS
Sbjct: 122 VPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSS 181

Query: 183 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGP 242
           SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++PRQDTIGP
Sbjct: 182 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGP 241

Query: 243 ICRTVSDAVYVLDAIVGFDPMDSEATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRHPFS 302
           ICRTVSDAVY+LDAIVGFDPMDSEATK AS FIP GGYKQFL KDGL GKRLGIVRHPF 
Sbjct: 242 ICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPFF 301

Query: 303 LLYPNESMAVSTFDHHVNFVLRKSGATIVDNLQISNVDVILNTFQSGELVAMVAEFKIAI 362
            LY N SMA+ TF+HHVN +LRKSGATIVDNLQISN+D IL+ + SGEL+A +AEFK+AI
Sbjct: 302 DLYTNGSMAIPTFEHHVN-LLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAI 361

Query: 363 NDYLKKLIHSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTDGIGEMEKEAISTMA 422
           NDYLK LIHSPVRSLADIIAFNNNHPELE M EYGQDAFLLS+QTDGIG+ E+EAIS MA
Sbjct: 362 NDYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMA 421

Query: 423 NLSRYGFEEMMKTYDLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFGGLKG 482
           NLSR GFE MMKTY+LDAMVTLGT AE+VLAIGGYPAISVPAGYEGNG+PFGILFGGL+G
Sbjct: 422 NLSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRG 481

Query: 483 TEPKLIEIAYAYEQATRVRRPPPL 507
           TE KLIEIAYA+EQAT VRRPPPL
Sbjct: 482 TETKLIEIAYAFEQATMVRRPPPL 504

BLAST of Tan0010121 vs. ExPASy TrEMBL
Match: A0A0A0K188 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009770 PE=4 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 8.6e-237
Identity = 427/514 (83.07%), Postives = 465/514 (90.47%), Query Frame = 0

Query: 1   MRVVFLLSAFILFAG-AHCSLFPIDEATVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPV 60
           M +VF LSA +LF G A+ S F IDEAT+AEIQ AFSQNKLTS QLLD+YL KI  LNPV
Sbjct: 1   MNIVFFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHLLNPV 60

Query: 61  LKSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIATKDRLNTTAGSFALL 120
           LKSVLE+NPDARAQAEAADRER LAGGKA GELHG+P+LLKD+IATKD LNTTAGSFALL
Sbjct: 61  LKSVLELNPDARAQAEAADRERLLAGGKARGELHGVPILLKDAIATKDLLNTTAGSFALL 120

Query: 121 GSVVPRDATVVDRLRNDGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPC 180
           GSVVPRDATVV RLRN GAVILGKTSLTEWY SRS +IP+GWCARGGQA+NPYG+GGDPC
Sbjct: 121 GSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSFEIPNGWCARGGQAVNPYGRGGDPC 180

Query: 181 GSSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDT 240
           GSSSGSAISVAANM AVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++PRQDT
Sbjct: 181 GSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDT 240

Query: 241 IGPICRTVSDAVYVLDAIVGFDPMDSEATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRH 300
           IGPICRTVSDAVYVL+AIVGFDPMD E TK AS FIPSGGYKQFL K+GL GKRLGIVRH
Sbjct: 241 IGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPSGGYKQFLRKNGLKGKRLGIVRH 300

Query: 301 PFSLLYPNESMAVSTFDHHVNFVLRKSGATIVDNLQISNVDVILNTFQSGELVAMVAEFK 360
           PFS LYPN S+A+ TF+ H+N +LRK GATIVDNLQISNVDVILN+++SGE +A++AEFK
Sbjct: 301 PFSDLYPNNSIAIPTFEQHLN-LLRKMGATIVDNLQISNVDVILNSYESGEFIAIIAEFK 360

Query: 361 IAINDYLKKLIHSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTDGIGEMEKEAIS 420
           +AINDYLKKLI SPVRSLADII+FNNNH ELE MKEYGQDAFLLSEQT+GIG MEKEAIS
Sbjct: 361 VAINDYLKKLIRSPVRSLADIISFNNNHAELEKMKEYGQDAFLLSEQTNGIGVMEKEAIS 420

Query: 421 TMANLSRYGFEEMMKTYDLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFGG 480
            MANLSR GFEE+MK  +LDAMVT+G G E+VLAIGGYP ISVPAGYE NGEPFGILFGG
Sbjct: 421 KMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGILFGG 480

Query: 481 LKGTEPKLIEIAYAYEQATRVRRPPPLLSKLSSL 514
           LKG+EPKLIEIAYAYEQAT VR PPPLLS++ SL
Sbjct: 481 LKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL 513

BLAST of Tan0010121 vs. ExPASy TrEMBL
Match: A0A1S3BIN0 (putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103490302 PE=4 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 3.3e-236
Identity = 431/512 (84.18%), Postives = 463/512 (90.43%), Query Frame = 0

Query: 3   VVFLLSAFILFAG-AHCSLFPIDEATVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVLK 62
           V F LSA +LF G A+ S F IDEAT+ EIQ AFSQNKLTS QLLD+YLNKI  LNPVLK
Sbjct: 36  VFFFLSAVLLFTGLANSSYFSIDEATIVEIQNAFSQNKLTSTQLLDYYLNKIHLLNPVLK 95

Query: 63  SVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIATKDRLNTTAGSFALLGS 122
           SVLE+NPDARAQAEAADRER LA GK+LGELHGIP+LLKD+IATKD LNTTAGSFALLGS
Sbjct: 96  SVLELNPDARAQAEAADRERLLACGKSLGELHGIPILLKDAIATKDLLNTTAGSFALLGS 155

Query: 123 VVPRDATVVDRLRNDGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCGS 182
           VVPRDATVV RLRN GAVILGKTSLTEW+ SRS KIP+GWCARGGQA+NPYGKGGDPCGS
Sbjct: 156 VVPRDATVVSRLRNAGAVILGKTSLTEWFKSRSSKIPNGWCARGGQAVNPYGKGGDPCGS 215

Query: 183 SSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIG 242
           SSGSAISVAANM AVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIG
Sbjct: 216 SSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIG 275

Query: 243 PICRTVSDAVYVLDAIVGFDPMDSEATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRHPF 302
           PICRTVSDAVYVL+AIVGFDPMD E TK AS FIPSGGYKQFL KDGL GKRLGIVRHPF
Sbjct: 276 PICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPSGGYKQFLRKDGLKGKRLGIVRHPF 335

Query: 303 SLLYPNESMAVSTFDHHVNFVLRKSGATIVDNLQISNVDVILNTFQSGELVAMVAEFKIA 362
           S LY N SMA+ TF+ H+N +LRK GATIVDNLQISNVDVILN ++SGE +A++AEFK+A
Sbjct: 336 SDLYSNNSMAIPTFEQHLN-LLRKMGATIVDNLQISNVDVILNPYESGEFIAIIAEFKLA 395

Query: 363 INDYLKKLIHSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTDGIGEMEKEAISTM 422
           +NDYLKKLI SPVRSLADII+FNN HPELENMKEYGQDAFLLSEQT+GIGEMEKEAIS M
Sbjct: 396 VNDYLKKLIQSPVRSLADIISFNNKHPELENMKEYGQDAFLLSEQTNGIGEMEKEAISRM 455

Query: 423 ANLSRYGFEEMMKTYDLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFGGLK 482
            NLSR GFEE+MK  +LDAMVT+GTG E+VLAIGGYP ISVPAGYE NGEPFGILFGGLK
Sbjct: 456 MNLSRNGFEELMKGNNLDAMVTIGTGIESVLAIGGYPGISVPAGYEENGEPFGILFGGLK 515

Query: 483 GTEPKLIEIAYAYEQATRVRRPPPLLSKLSSL 514
           G+EPKLIEIAYAYEQAT VR  PPLLS++SSL
Sbjct: 516 GSEPKLIEIAYAYEQATMVRE-PPLLSRISSL 545

BLAST of Tan0010121 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 494.6 bits (1272), Expect = 9.7e-140
Identity = 268/490 (54.69%), Postives = 335/490 (68.37%), Query Frame = 0

Query: 19  SLFPIDEATVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVLKSVLEVNPDARAQAEAAD 78
           S F I EAT+ +I++AF++ +LTS+QL++ YL  I  LNP+L +V+E NPDA  QAE AD
Sbjct: 36  STFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAISKLNPILHAVIETNPDALIQAEIAD 95

Query: 79  RERELAGGKALGELHGIPVLLKDSIATKDRLNTTAGSFALLGSVVPRDATVVDRLRNDGA 138
           RER+L     L  LHG+PVLLKDSI+TKD+LNTTAGSFALLGSVV RDA VV RLR  GA
Sbjct: 96  RERDLKNTTKLPILHGVPVLLKDSISTKDKLNTTAGSFALLGSVVARDAGVVKRLRESGA 155

Query: 139 VILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSL 198
           VILGK SL+EW   RS  IP GW A                                   
Sbjct: 156 VILGKASLSEWAHFRSFSIPDGWSA----------------------------------- 215

Query: 199 GTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIV 258
                         NSVVGIKP+VGLTSRAGV+PIS RQD+IGPICRTVSDAV++LDAIV
Sbjct: 216 -----------PSQNSVVGIKPSVGLTSRAGVVPISLRQDSIGPICRTVSDAVHLLDAIV 275

Query: 259 GFDPMDSEATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRHPFSLLYPNESMAVSTFDHH 318
           G+DP+D EATK AS FIP GGYKQFL   GL GKRLGIV    SLL           DHH
Sbjct: 276 GYDPLD-EATKTASEFIPEGGYKQFLTTSGLKGKRLGIVMKHSSLL-----------DHH 335

Query: 319 VNFVLRKSGATIVDNLQISNVDVILNTFQSGELVAMVAEFKIAINDYLKKLIHSPVRSLA 378
           +   LR+ GA +++NL I N++VI+    SGE +A++AEFK+++N YLK+L+ SPVRSLA
Sbjct: 336 IK-TLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAEFKMSLNAYLKELVKSPVRSLA 395

Query: 379 DIIAFNNNHPELENMKEYGQDAFLLSEQTDGIGEMEKEAISTMANLSRYGFEEMMKTYDL 438
           D+IA+N    E E +KE+GQ+ FL +E T G+GE EK A+  M  LSR G E++++   L
Sbjct: 396 DVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTALQKMKELSRNGIEKLIEENKL 455

Query: 439 DAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFGGLKGTEPKLIEIAYAYEQAT 498
           DA+VTLG+   +VLAIGGYP I+VPAGY+  G P+GI FGGL+ +EPKLIEIA+A+EQAT
Sbjct: 456 DAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISFGGLRFSEPKLIEIAFAFEQAT 466

Query: 499 RVRRPPPLLS 509
            +R+PP  ++
Sbjct: 516 LIRKPPKFIA 466

BLAST of Tan0010121 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-27
Identity = 142/509 (27.90%), Postives = 232/509 (45.58%), Query Frame = 0

Query: 41  TSRQLLDHYLNKIDALNPVLKSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLK 100
           T+ ++   YL++I    P LK  L V+ +    A+  D  + +A G+ LG L G+ + +K
Sbjct: 59  TAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQEID--QRIAKGEELGPLAGVLIGVK 118

Query: 101 DSIATKDRLNTTAGSFALLGSVVPRDATVVDRLRNDGAVILGKTSLTEWYGSRSLKIPHG 160
           D+I T+  + +TA S  L     P DAT V +++  G +++GKT++ E +G  S      
Sbjct: 119 DNICTQG-MPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDE-FGMGSTTEASA 178

Query: 161 WCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKP 220
           +        NP+     P GSS GSA +VAA    VSLG++T GS+  PA +  VVG+KP
Sbjct: 179 FQV----TANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKP 238

Query: 221 TVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDSEATKLASAFIPSGGY 280
           T G  SR G++  +   D IG    TV+DA  +L AI G+D  DS ++K       S   
Sbjct: 239 TYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTSSKQDVPEFQS--- 298

Query: 281 KQFLNKD-----GLLGKRLGIVRHPFSLLYPNESMAVSTFDHHVNFVLR---KSGATIVD 340
            QFL+ D      L G ++GI+R               T +  V+  +R   +  A+ ++
Sbjct: 299 -QFLSVDHFESKPLNGVKVGIIR--------------ETLEDGVDSGVRSATQEAASHLE 358

Query: 341 NLQISNVDVILNTFQSGELVAMVAEFKIAINDYLKKLI-HSPVRSLADIIAFN-NNHPEL 400
            L     +V L +F  G    + A + IA ++    L  +  VR    ++A   N   E 
Sbjct: 359 ALGCILTEVSLPSFSLG----LPAYYVIASSESSSNLSRYDGVRYGNQVMAEELNKLYEC 418

Query: 401 ENMKEYGQDA---FLLSEQTDGIGEMEK--EAISTMANLSRYGFEEMMKTYDL------- 460
              + +G +     L+       G  +   +    +  L R  F+  ++  D+       
Sbjct: 419 SRGEGFGGEVKMRILMGTYALSAGYYDAYYKRAQQVRTLIRKDFKAALEQNDILISPAAP 478

Query: 461 DAMVTLGTGAETVLA------------IGGYPAISVPAG-YEG--NGEPFGILFGGLKGT 512
            A   +G   +  LA            + G PA+ +P G  EG  +G P G+   G    
Sbjct: 479 SAAYKIGEKKDDPLAMYAGDIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFD 537

BLAST of Tan0010121 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 119.8 bits (299), Expect = 6.5e-27
Identity = 83/245 (33.88%), Postives = 127/245 (51.84%), Query Frame = 0

Query: 27  TVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVLKSVLEVNPDARAQAEAADRERELAGG 86
           +V E+       ++TS++L+  YL ++   N VL++V+    +  A  +A + +  L+ G
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEEL-AYKQAKEADDLLSQG 263

Query: 87  KALGELHGIPVLLKDSIATKDRLNTTAGSFALLGSVVPRDATVVDRLRNDGAVILGKTSL 146
             LG LHGIP  LKD +A      TT GS +     +  +A V  RL+  GAV++ K   
Sbjct: 264 TYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLV- 323

Query: 147 TEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGSI 206
                + S+     W   GG+  NP+       GSS+G A S +A M   ++G+ET GS+
Sbjct: 324 -----TGSMAYDDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSM 383

Query: 207 LCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDSE 266
             PA    +  ++PT G   R GV+ IS   D +GP CRT +D   +LDAI G DP D  
Sbjct: 384 TYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLS 438

Query: 267 ATKLA 272
           + ++A
Sbjct: 444 SREIA 438

BLAST of Tan0010121 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 110.9 bits (276), Expect = 3.0e-24
Identity = 82/245 (33.47%), Postives = 124/245 (50.61%), Query Frame = 0

Query: 27  TVAEIQLAFSQNKLTSRQLLDHYLNKIDALNPVLKSVLEVNPDARAQAEAADRERELAGG 86
           +V E+       ++TS++L+  YL ++   N VL++V+    +  A  +A + +  L+ G
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEEL-AYKQAKEADDLLSQG 263

Query: 87  KALGELHGIPVLLKDSIATKDRLNTTAGSFALLGSVVPRDATVVDRLRNDGAVILGKTSL 146
             LG LHGIP  LKD +A      TT GS +     +  +A V  RL+  GAV++ K   
Sbjct: 264 TYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLV- 323

Query: 147 TEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAANMAAVSLGTETDGSI 206
                + S+     W   GG+  NP+       GSS+G A       A+ S G+ET GS+
Sbjct: 324 -----TGSMAYDDIWF--GGRTRNPWNIEEFSTGSSAGPA-------ASTSAGSETAGSM 383

Query: 207 LCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVYVLDAIVGFDPMDSE 266
             PA    +  ++PT G   R GV+ IS   D +GP CRT +D   +LDAI G DP D  
Sbjct: 384 TYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLS 431

Query: 267 ATKLA 272
           + ++A
Sbjct: 444 SREIA 431

BLAST of Tan0010121 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 85.9 bits (211), Expect = 1.0e-16
Identity = 114/471 (24.20%), Postives = 183/471 (38.85%), Query Frame = 0

Query: 73  QAEAADRERELAGGKALGELHGIPVLLKDSIATKDRLNTTAGSFALLGSVVPRDATVVDR 132
           QAEA+ R  E   G  +  L GI V +KD I           ++      V +D+ VV +
Sbjct: 180 QAEASTRRFE--QGNPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSK 239

Query: 133 LRNDGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCGSSSGSAISVAAN 192
           LR+ GA++LGK ++ E     +     G  +  G   NP+       GSSSGSA  VAA 
Sbjct: 240 LRSCGAILLGKANMHELGMGTT-----GNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAG 299

Query: 193 MAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPICRTVSDAVY 252
           + + +LGT+  GS+  P+    + G+K T G T   G +      + IGP+  ++ DA  
Sbjct: 300 LCSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFL 359

Query: 253 VLDAIVGFDPMDSEATKLASAFIPSGGYKQFLNKDGLLGKRLGIVRHPFSLLYPNESMAV 312
           V  AI+G    D    K +    P                                    
Sbjct: 360 VYAAILGSSSADRYNLKPSPPCFPK----------------------------------- 419

Query: 313 STFDHHVNFVLRKSGATIVDNLQISNVDVILNTFQSGELVAMVAE-FKIAINDYLKKLIH 372
                    +L  +G+  + +L++       N   S ++     +  K+  N++  K++ 
Sbjct: 420 ---------LLSHNGSNAIGSLRLGKYTKWFNDVSSSDISDKCEDILKLLSNNHGCKVVE 479

Query: 373 SPVRSLADIIA---FNNNHPELENMKEY---GQDAFLLSEQTDGIGEMEKEAIS---TMA 432
             V  L ++ A    +   P L ++  Y   G+++ L  +           + S      
Sbjct: 480 IVVPELEEMRAAHVISIGSPTLSSLTPYCEAGKNSKLSYDTRTSFAIFRSFSASDYIAAQ 539

Query: 433 NLSRYGFEEMMKTY-DLDAMVTLGTG-------------AET-----------VLAIG-- 492
            L R   E  +  + D+D +VT  TG              ET           VLA    
Sbjct: 540 CLRRRLMEYHLNIFKDVDVIVTPTTGMTAPVIPPDALKNGETNIQVTTDLMRFVLAANLL 599

Query: 493 GYPAISVPAGYEGNGEPFGILFGGLKGTEPKLIEIAYAYEQATRVRRPPPL 507
           G+PAISVP GY+  G P G+   G    E  ++ +A A E+   V + P +
Sbjct: 600 GFPAISVPVGYDKEGLPIGLQIMGRPWAEATVLGLAAAVEELAPVTKKPAI 599