Tan0010065 (gene) Snake gourd v1

Overview


Name	Tan0010065
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	gibberellin 2-beta-dioxygenase-like
Location	LG05: 77587547 .. 77589499 (+)
RNA-Seq Expression	Tan0010065
Synteny	Tan0010065

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCAAATTCAAACTTCTTCTTCTTCTTCTTCTTTGAAAACCTTTTTCTTTTTCAATCATGGTGGTTCAATCTCCGCTGGTGTTCGACCATTTTGCTCCGATCAAATCCTGCAAACGCACCGCTCTGTTCACCGGAATTCCGGTGATCGATCTTTTCGACCCAGAAGCCAAAACCCATATCGTCAAAGCCTGTGAGGATTTTGGCTTCTTCAAGGTCGTCAACCATGGCGTCTCCGACGACCTTATCGGAGACCTGGAAGCCCAAGCTCAGAAATTCTTCCAACTCCCTCAATCGGAGAAGGAAAAAGCAGGGCCACCTGACCCATTTGGATACGGCAGCAAGAGAATTGGCCCAAACGGAGATGTGGGTTGGATTGAATACCTCCTCCTCAACACCAATCACGAACTCATTTCTGAAAAATCCCTCTTCATTCTCCGCGAAAATCCAGAGATTTTCAGGCAAGAAAAAACAGATTGCTTGCTCTGTTTTTGTTTGCTCTGTTTTTGGATTGCTCTGTTTTCTGATTTGTTGTTGTATTGGTTGTAGGTGTGCGGTGGAGGAGTATATACTGGCAGTGAAGGAGATGGCTTGTGAAGTGCTGGAAGCAATGGCGGAAGGGCTGGGGATAGAGAAAAGCGCTCTGAGCAGGCTGTTGAAGGACGAGAGAAGTGACTGTTGTTTCAGGCTGAACCATTATCCGCCATGCCCGGAGCTAGAAGCTTTGAGTGGCAGAAATTTGATTGGGTTTGGGGAACATACAGACCCACAAATAATATCTGTCTTGAGATCCAATAACACAACCGGACTCCATATCTGTTTGAGAGATGGAAATTGGGTTTCAGTCCCACCTGATCAGACCTCCTTTTTCATCATTGTTGGTGACTCATTGCAGGTACAATCCCCTTTCTTTCTCTTTCTCTTCCTCTTCCTCTTCTTCTTCCTCTGCCATGGAAATGGAAATCAAGGTCTATTCTGTGTTTTTTTTTTTTTGTTTAAAAAACTACCCTTTTACTTAGGGTTCTTGTTGGGTGGATTTTGATGATTTGTGTATTTGTTTGTGTTGGTACAGGTGATGACTAATGGGAGGTTTAAGAGTGTGAAGCATAAGGTTTTAGCTGATCCAATAAAATCAAGATTTTCAATGGTCTACTTTGGTGGGCCACCTTTGAATGAAAAGATTGCACCTTTACCATCACTATTGGAGGAGGGAGAGCAAAGTTTGTATAGAGAATTTACTTGGTGGGATTACAAAAACTCTGCATATAAATCAAGACTTGCTGATTGTAGGTTAGGCCCTTTTGAGATTAAAAAAAAATCTCACCCCTTTTTGTTGACTTGAGAAACCCCCCACAAAACACACCTTTCAAAATTATGTCACAATGCAAAATAAATAGAAACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGCTCTTTAGGGATCTTTTTGAAAATATGTGAAATTCTTCCTGTCATTTCTCTGTTGTTCTTTTATGTGGGTATGGTTTGAGAAAGCAAAAAAGAAAAAGGTTATGAATGATAAAATTATGGAGGAATAATAGGGGAATATTTGGCTTAAAAAAGGAAGAAAAGAAAGTTTAAATTAGATTAGAGAGGAAAGGTAGAGATAAAGATGGTAGGGATTTGGGAAGAATAGAATAGATTTGTTTTTGTTTTTGTTTTTGTGTTTGTCACAATATGTTAGTTTAGTTGTTCTTTTTGTTAAATATTTGCTTTTAGGTTTAAATGTAAGAGTGAGGACAGCCACCTTCCATCCTCCAATCCCATTTAATACTCTTGTCACCATGTCCAAATTACACCCTAATGTATTTTCCCCAAAATACCCCTCACATTGTAAATATACTTGTCACATAAACTTCCCCTTCCCCTCTTGTATCAAAGCTTGTGGATTTCAATGTTATATTATATATGCTTCTTACAAT

mRNA sequence

TTCAAATTCAAACTTCTTCTTCTTCTTCTTCTTTGAAAACCTTTTTCTTTTTCAATCATGGTGGTTCAATCTCCGCTGGTGTTCGACCATTTTGCTCCGATCAAATCCTGCAAACGCACCGCTCTGTTCACCGGAATTCCGGTGATCGATCTTTTCGACCCAGAAGCCAAAACCCATATCGTCAAAGCCTGTGAGGATTTTGGCTTCTTCAAGGTCGTCAACCATGGCGTCTCCGACGACCTTATCGGAGACCTGGAAGCCCAAGCTCAGAAATTCTTCCAACTCCCTCAATCGGAGAAGGAAAAAGCAGGGCCACCTGACCCATTTGGATACGGCAGCAAGAGAATTGGCCCAAACGGAGATGTGGGTTGGATTGAATACCTCCTCCTCAACACCAATCACGAACTCATTTCTGAAAAATCCCTCTTCATTCTCCGCGAAAATCCAGAGATTTTCAGGTGTGCGGTGGAGGAGTATATACTGGCAGTGAAGGAGATGGCTTGTGAAGTGCTGGAAGCAATGGCGGAAGGGCTGGGGATAGAGAAAAGCGCTCTGAGCAGGCTGTTGAAGGACGAGAGAAGTGACTGTTGTTTCAGGCTGAACCATTATCCGCCATGCCCGGAGCTAGAAGCTTTGAGTGGCAGAAATTTGATTGGGTTTGGGGAACATACAGACCCACAAATAATATCTGTCTTGAGATCCAATAACACAACCGGACTCCATATCTGTTTGAGAGATGGAAATTGGGTTTCAGTCCCACCTGATCAGACCTCCTTTTTCATCATTGTTGGTGACTCATTGCAGGTGATGACTAATGGGAGGTTTAAGAGTGTGAAGCATAAGGTTTTAGCTGATCCAATAAAATCAAGATTTTCAATGGTCTACTTTGGTGGGCCACCTTTGAATGAAAAGATTGCACCTTTACCATCACTATTGGAGGAGGGAGAGCAAAGTTTGTATAGAGAATTTACTTGGTGGGATTACAAAAACTCTGCATATAAATCAAGACTTGCTGATTGTAGGTTAGGCCCTTTTGAGATTAAAAAAAAATCTCACCCCTTTTTGTTGACTTGAGAAACCCCCCACAAAACACACCTTTCAAAATTATGTCACAATGCAAAATAAATAGAAACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGCTCTTTAGGGATCTTTTTGAAAATATGTGAAATTCTTCCTGTCATTTCTCTGTTGTTCTTTTATGTGGGTATGGTTTGAGAAAGCAAAAAAGAAAAAGGTTATGAATGATAAAATTATGGAGGAATAATAGGGGAATATTTGGCTTAAAAAAGGAAGAAAAGAAAGTTTAAATTAGATTAGAGAGGAAAGGTAGAGATAAAGATGGTAGGGATTTGGGAAGAATAGAATAGATTTGTTTTTGTTTTTGTTTTTGTGTTTGTCACAATATGTTAGTTTAGTTGTTCTTTTTGTTAAATATTTGCTTTTAGGTTTAAATGTAAGAGTGAGGACAGCCACCTTCCATCCTCCAATCCCATTTAATACTCTTGTCACCATGTCCAAATTACACCCTAATGTATTTTCCCCAAAATACCCCTCACATTGTAAATATACTTGTCACATAAACTTCCCCTTCCCCTCTTGTATCAAAGCTTGTGGATTTCAATGTTATATTATATATGCTTCTTACAAT

Coding sequence (CDS)

ATGGTGGTTCAATCTCCGCTGGTGTTCGACCATTTTGCTCCGATCAAATCCTGCAAACGCACCGCTCTGTTCACCGGAATTCCGGTGATCGATCTTTTCGACCCAGAAGCCAAAACCCATATCGTCAAAGCCTGTGAGGATTTTGGCTTCTTCAAGGTCGTCAACCATGGCGTCTCCGACGACCTTATCGGAGACCTGGAAGCCCAAGCTCAGAAATTCTTCCAACTCCCTCAATCGGAGAAGGAAAAAGCAGGGCCACCTGACCCATTTGGATACGGCAGCAAGAGAATTGGCCCAAACGGAGATGTGGGTTGGATTGAATACCTCCTCCTCAACACCAATCACGAACTCATTTCTGAAAAATCCCTCTTCATTCTCCGCGAAAATCCAGAGATTTTCAGGTGTGCGGTGGAGGAGTATATACTGGCAGTGAAGGAGATGGCTTGTGAAGTGCTGGAAGCAATGGCGGAAGGGCTGGGGATAGAGAAAAGCGCTCTGAGCAGGCTGTTGAAGGACGAGAGAAGTGACTGTTGTTTCAGGCTGAACCATTATCCGCCATGCCCGGAGCTAGAAGCTTTGAGTGGCAGAAATTTGATTGGGTTTGGGGAACATACAGACCCACAAATAATATCTGTCTTGAGATCCAATAACACAACCGGACTCCATATCTGTTTGAGAGATGGAAATTGGGTTTCAGTCCCACCTGATCAGACCTCCTTTTTCATCATTGTTGGTGACTCATTGCAGGTGATGACTAATGGGAGGTTTAAGAGTGTGAAGCATAAGGTTTTAGCTGATCCAATAAAATCAAGATTTTCAATGGTCTACTTTGGTGGGCCACCTTTGAATGAAAAGATTGCACCTTTACCATCACTATTGGAGGAGGGAGAGCAAAGTTTGTATAGAGAATTTACTTGGTGGGATTACAAAAACTCTGCATATAAATCAAGACTTGCTGATTGTAGGTTAGGCCCTTTTGAGATTAAAAAAAAATCTCACCCCTTTTTGTTGACTTGA

Protein sequence

MVVQSPLVFDHFAPIKSCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSDDLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISEKSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIEKSALSRLLKDERSDCCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTSFFIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQSLYREFTWWDYKNSAYKSRLADCRLGPFEIKKKSHPFLLT

Homology

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BLAST of Tan0010065 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 9.6e-133
Identity = 230/328 (70.12%), Postives = 268/328 (81.71%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIKSCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVV S    + F  +K  K T LFTGIPV+DL  P+AK  IV AC DFGFFK+VNHGV  
Sbjct: 1   MVVLSQPALNQFFLLKPFKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHGVPL 60

Query: 61  DLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           +L+ +LE +A +FF+  QSEK++AGPPDPFGYGSKRIGPNGDVGW+EYLLLNTN ++IS 
Sbjct: 61  ELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISP 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGI-EKSALSRLLKDERSDCCF 180
           KSL I RENP  FR  VE YI AVK M   VLE MAEGLGI +++ LSRLLKDE+SD CF
Sbjct: 121 KSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCF 180

Query: 181 RLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTS 240
           RLNHYPPCPE++AL+ RNL+GFGEHTDPQIISVLRSN+T+GL ICL DG WVSVPPDQTS
Sbjct: 181 RLNHYPPCPEVQALN-RNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTS 240

Query: 241 FFIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQS 300
           FFI VGD+LQVMTNGRFKSVKH+VLAD  KSR SM+YFGGP L+E IAPLPS++ +GE+ 
Sbjct: 241 FFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKGEEC 300

Query: 301 LYREFTWWDYKNSAYKSRLADCRLGPFE 328
           LY+EFTW +YK +AY SRLAD RL PF+
Sbjct: 301 LYKEFTWCEYKKAAYTSRLADNRLAPFQ 327

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BLAST of Tan0010065 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 2.4e-115
Identity = 205/331 (61.93%), Postives = 256/331 (77.34%), Query Frame = 0


Query: 1   MVVQSPLVFD-HFAPIKSCKRTALFT--GIPVIDLFDPEAKTHIVKACEDFGFFKVVNHG 60
           +V+  P+  D H + I + K   + T   IPV++L DPEAKT IVKACE+FGFFKVVNHG
Sbjct: 2   VVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHG 61

Query: 61  VSDDLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHEL 120
           V  +L+  LE +A  FF LPQS K +AGPP+P+GYG+KRIGPNGDVGWIEYLLLN N +L
Sbjct: 62  VRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQL 121

Query: 121 ISEKSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIE-KSALSRLLKDERSD 180
            S K+  + R+ P+IFR +VEEY+  +KE++ +VLE +AE LGIE +  LS++L+DE+SD
Sbjct: 122 SSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSD 181

Query: 181 CCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPD 240
            C RLNHYP   E      +  +GFGEHTDPQIISVLRSNNT GL IC++DG+WV+VPPD
Sbjct: 182 SCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPD 241

Query: 241 QTSFFIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEG 300
            +SFFI VGD+LQVMTNGRFKSVKH+VLAD  +SR SM+YFGGPPL++KIAPLP L+ E 
Sbjct: 242 HSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVPEQ 301

Query: 301 EQSLYREFTWWDYKNSAYKSRLADCRLGPFE 328
           +  LY+EFTW  YK+SAYKS+L D RLG FE
Sbjct: 302 DDWLYKEFTWSQYKSSAYKSKLGDYRLGLFE 330

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BLAST of Tan0010065 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 5.9e-114
Identity = 199/328 (60.67%), Postives = 257/328 (78.35%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIKSCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MV+ S    + +  +++       + IP++DL  P+AKT IVKACEDFGFFKV+NHG+  
Sbjct: 1   MVLLSKPTSEQYTYVRNNMPITFSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHGIPL 60

Query: 61  DLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           D I  LE++A KFF LPQ+EKEKAGP +PFGYG+KRIG NGD+GWIEYLLL TN     +
Sbjct: 61  DAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTN----QD 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIE-KSALSRLLKDERSDCCF 180
            +  +  E+ + FR  +++Y  A++ MACE+L+ MAEGL I+ K+  S+L+ D++SDC F
Sbjct: 121 HNFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLF 180

Query: 181 RLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTS 240
           R+NHYP CPEL A++G NLIGFGEHTDPQIIS+LRSNNT+G  I LRDG+W+SVPPD +S
Sbjct: 181 RVNHYPACPEL-AINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSS 240

Query: 241 FFIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQS 300
           FFI VGDSLQVMTNGRFKSV+H+VLA+ I  R SM+YF GPPL+EKIAPLPSL+ +G++S
Sbjct: 241 FFINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPSLM-KGKES 300

Query: 301 LYREFTWWDYKNSAYKSRLADCRLGPFE 328
           LY+EFTW++YK+S Y SRLAD RLG +E
Sbjct: 301 LYKEFTWFEYKSSTYGSRLADNRLGNYE 322

[Close]

BLAST of Tan0010065 vs. ExPASy Swiss-Prot
Match: O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.2e-105
Identity = 197/340 (57.94%), Postives = 241/340 (70.88%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIK-SCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVS 60
           ++V  P  FD    +   CK   +   IPVIDL D +AKT IVKACE+FGFFKV+NHGV 
Sbjct: 2   VIVLQPASFDSNLYVNPKCKPRPVL--IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVR 61

Query: 61  DDLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELIS 120
            DL+  LE +A  FF L  S K+KAGPPDPFGYG+KRIGPNGD+GW+EY+LLN N  L S
Sbjct: 62  PDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLES 121

Query: 121 EKSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIE-KSALSRLLKDERSDCC 180
            K+  I R  P IFR AVEEYI  +K M+ + LE + E L IE K  LSRL+K + SD C
Sbjct: 122 HKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSC 181

Query: 181 FRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQT 240
            R+NHY   PE E    +  IGFGEHTDPQ+IS+LRSN+T GL IC++DG WV V PD +
Sbjct: 182 LRMNHY---PEKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHS 241

Query: 241 SFFIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQ 300
           SFF++VGD+LQVMTNGRFKSVKH+V+ +  +SR SM+YF GPPL+EKIAPL  L+ + + 
Sbjct: 242 SFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDD 301

Query: 301 SLYREFTWWDYKNSAYKSRLADCRLGPFEIKKKSHPFLLT 339
            LY EFTW  YK SAYK++L D RLG FE   K  PF L+
Sbjct: 302 CLYNEFTWSQYKLSAYKTKLGDYRLGLFE---KRPPFSLS 333

[Close]

BLAST of Tan0010065 vs. ExPASy Swiss-Prot
Match: Q8S0S6 (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 3.6e-103
Identity = 185/330 (56.06%), Postives = 240/330 (72.73%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIKSCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           +V+  P   DH   ++S     +F+G+PV+DL  P A   +V ACE +GFFKVVNHGV+ 
Sbjct: 2   VVLAGPPAVDHIPLLRSPDPGDVFSGVPVVDLGSPGAARAVVDACERYGFFKVVNHGVAT 61

Query: 61  DLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           D +   E++A +FF   Q +K+++GP  PFGYGSKRIG NGD+GW+EYLLL  +   +++
Sbjct: 62  DTMDKAESEAVRFFSQTQPDKDRSGPAYPFGYGSKRIGFNGDMGWLEYLLLALDDASLAD 121

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGI-EKSALSRLLKDERSDCCF 180
                   +  +FR A+ EYI  V+++A  V+EAM+EGLGI +  ALS L+  E SD  F
Sbjct: 122 ACTV---PSCAVFRAALNEYISGVRKVAVRVMEAMSEGLGIAQADALSALVTAEGSDQVF 181

Query: 181 RLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTS 240
           R+NHYPPC  L+ L G ++ GFGEHTDPQ++SVLRSN T+GL I LRDG WVSVP D+ S
Sbjct: 182 RVNHYPPCRALQGL-GCSVTGFGEHTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSDRDS 241

Query: 241 FFIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQS 300
           FF+ VGDSLQV+TNGRFKSVKH+V+A+ +KSR S +YFGGPPL ++IAPLP LL EGEQS
Sbjct: 242 FFVNVGDSLQVLTNGRFKSVKHRVVANSLKSRVSFIYFGGPPLAQRIAPLPQLLGEGEQS 301

Query: 301 LYREFTWWDYKNSAYKSRLADCRLGPFEIK 330
           LY+EFTW +YK +AYKSRL D RL  FE K
Sbjct: 302 LYKEFTWDEYKKAAYKSRLGDNRLAQFEKK 327

[Close]

BLAST of Tan0010065 vs. NCBI nr
Match: KAG7019518.1 (Gibberellin 2-beta-dioxygenase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 648.7 bits (1672), Expect = 2.8e-182
Identity = 310/329 (94.22%), Postives = 317/329 (96.35%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIKSCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQS LVFDHFAPIKSCKRTALFTGIPVI+L DPEAKT IVKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVINLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  DLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
            LIGDLEAQA KFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEY+LLNTNHELISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPE+FRCAVEEYILAVKEMACEVLEAMA GL IEKSALSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEVFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT GLHICLRDGNWVSVPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FI VGDSLQVMTNGRFKSVKHKVLADPIKSR SM+YFGGPPLNEKIAPLPSL+EEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIK 330
           YREFTWWDYKNSAYKSRLADCRLGPFEIK
Sbjct: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIK 329

[Close]

BLAST of Tan0010065 vs. NCBI nr
Match: XP_023001443.1 (gibberellin 2-beta-dioxygenase-like [Cucurbita maxima] >CBL59328.1 Gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 648.7 bits (1672), Expect = 2.8e-182
Identity = 311/329 (94.53%), Postives = 316/329 (96.05%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIKSCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQS LVFDHFAPIKSCKRTALFTGIPVIDL DPEAKT IVKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  DLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
            LIGDLEAQA KFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEY+LLNTNHELISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMA GL IEKSALSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT GLHICLRDGNWVSVPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FI VGDSLQVMTNGRFKSVKHKVLADPIKSR SM+YFGGPPLNEKIAPLPSL+EEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIK 330
           YREFTWWDYK SAYKSRLADCRLGPFEIK
Sbjct: 301 YREFTWWDYKTSAYKSRLADCRLGPFEIK 329

[Close]

BLAST of Tan0010065 vs. NCBI nr
Match: XP_022927050.1 (gibberellin 2-beta-dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 647.5 bits (1669), Expect = 6.3e-182
Identity = 310/329 (94.22%), Postives = 316/329 (96.05%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIKSCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQS LVFDHFAPIKSCKRTALFTGIPVIDL DPEAKT IVKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  DLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
            LIGDLEAQA KFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEY+LLNTNHELISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPE+FRCAVEEYILAVKEMACEVLEAMA GL IEKSALS LLKDERSDCCFR
Sbjct: 121 KSLFILRENPEVFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSWLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT GLHICLRDGNWVSVPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FI VGDSLQVMTNGRFKSVKHKVLADPIKSR SM+YFGGPPLNEKIAPLPSL+EEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIK 330
           YREFTWWDYKNSAYKSRLADCRLGPFEIK
Sbjct: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIK 329

[Close]

BLAST of Tan0010065 vs. NCBI nr
Match: XP_023519238.1 (gibberellin 2-beta-dioxygenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 647.1 bits (1668), Expect = 8.3e-182
Identity = 311/329 (94.53%), Postives = 316/329 (96.05%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIKSCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQS LVFDHFAPIKSCKRTALFTGIPVIDL DPEAKT IVKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  DLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
            LIGDLEAQA KFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEY+LLNTNHELISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPEIFR AVEEYILAVKEMACEVLEAMA GL IEKSALSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRSAVEEYILAVKEMACEVLEAMALGLRIEKSALSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT GLHICLRDGNWVSVPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FI VGDSLQVMTNGRFKSVKHKVLADPIKSR SM+YFGGPPLNEKIAPLPSL+EEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIK 330
           YREFTWWDYKNSAYKSRLADCRLGPFEIK
Sbjct: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIK 329

[Close]

BLAST of Tan0010065 vs. NCBI nr
Match: XP_038893915.1 (gibberellin 2-beta-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 646.0 bits (1665), Expect = 1.8e-181
Identity = 310/329 (94.22%), Postives = 314/329 (95.44%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIKSCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQS LVFDHFAPIKSCKRTALFTGIPVIDL DPEAKTHIVKACEDFGFFKV NHGVSD
Sbjct: 32  MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSD 91

Query: 61  DLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           DLIG LEAQA KFF LPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEY+LLNTN +LISE
Sbjct: 92  DLIGALEAQALKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISE 151

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIEKSALSRLLKDERSDCCFR 180
           KS  I RENPEIFRC VEEYILAVKEMACEVLE MAEGL IEKSALSRLLKDERSDCCFR
Sbjct: 152 KSFSIFRENPEIFRCLVEEYILAVKEMACEVLEVMAEGLRIEKSALSRLLKDERSDCCFR 211

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPD TSF
Sbjct: 212 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 271

Query: 241 FIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FI VGDSLQVMTNGRFKSVKHKVLADPIKSRFSM+YFGGPPLNEKIAPLPSLLE+GEQSL
Sbjct: 272 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQSL 331

Query: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIK 330
           YREFTWWDYKNSAYKSRLADCRLGPFEIK
Sbjct: 332 YREFTWWDYKNSAYKSRLADCRLGPFEIK 360

[Close]

BLAST of Tan0010065 vs. ExPASy TrEMBL
Match: I0J1I9 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox3 PE=2 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.4e-182
Identity = 311/329 (94.53%), Postives = 316/329 (96.05%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIKSCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQS LVFDHFAPIKSCKRTALFTGIPVIDL DPEAKT IVKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  DLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
            LIGDLEAQA KFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEY+LLNTNHELISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMA GL IEKSALSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT GLHICLRDGNWVSVPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FI VGDSLQVMTNGRFKSVKHKVLADPIKSR SM+YFGGPPLNEKIAPLPSL+EEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIK 330
           YREFTWWDYK SAYKSRLADCRLGPFEIK
Sbjct: 301 YREFTWWDYKTSAYKSRLADCRLGPFEIK 329

[Close]

BLAST of Tan0010065 vs. ExPASy TrEMBL
Match: A0A6J1EMS7 (gibberellin 2-beta-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111433993 PE=3 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 3.1e-182
Identity = 310/329 (94.22%), Postives = 316/329 (96.05%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIKSCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQS LVFDHFAPIKSCKRTALFTGIPVIDL DPEAKT IVKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  DLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
            LIGDLEAQA KFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEY+LLNTNHELISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPE+FRCAVEEYILAVKEMACEVLEAMA GL IEKSALS LLKDERSDCCFR
Sbjct: 121 KSLFILRENPEVFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSWLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT GLHICLRDGNWVSVPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FI VGDSLQVMTNGRFKSVKHKVLADPIKSR SM+YFGGPPLNEKIAPLPSL+EEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIK 330
           YREFTWWDYKNSAYKSRLADCRLGPFEIK
Sbjct: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIK 329

[Close]

BLAST of Tan0010065 vs. ExPASy TrEMBL
Match: A0A6J1K680 (gibberellin 2-beta-dioxygenase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490445 PE=3 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 6.0e-178
Identity = 304/329 (92.40%), Postives = 310/329 (94.22%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIKSCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQS LVFDHFAPIKSCK TALFTGIPVIDL DPEAKTHIVKACEDFGFFKVVNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60

Query: 61  DLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           DLIG LEAQA KFF LPQSEKEKAGP DPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE
Sbjct: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVK+MACEVLEAMAEGLGIEK  LSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDG+WV VPPDQ SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240

Query: 241 FIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FI VGDSLQVMTNGRF SVKHKVLADP K RFSM+YFGGPPLNEKIAPL S+LEEGEQ L
Sbjct: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASILEEGEQRL 300

Query: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIK 330
           Y+EFTWWDYKNSAYKSRLA CRL PFE+K
Sbjct: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELK 329

[Close]

BLAST of Tan0010065 vs. ExPASy TrEMBL
Match: A0A6J1GTD6 (gibberellin 2-beta-dioxygenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457257 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.5e-176
Identity = 302/329 (91.79%), Postives = 309/329 (93.92%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIKSCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQS LVFDHFAPIKSCK TALFTGIPVIDL DPEAKTHIVKACEDFGFFKVVNHGVS+
Sbjct: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSN 60

Query: 61  DLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           +LIG LEAQA KFF LPQSEKEKAGP DPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE
Sbjct: 61  ELIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVK+MACEVLEAMAEGLGIEK  LSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDG+WV VPPDQ SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240

Query: 241 FIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FI VGDSLQVMTNGRF SVKHKVLADP K RFSM+YFGGPPLNEKIAPL SLL EGEQ L
Sbjct: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLVEGEQRL 300

Query: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIK 330
           Y+EFTWWDYKNSAYKSRLA CRL PFE+K
Sbjct: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELK 329

[Close]

BLAST of Tan0010065 vs. ExPASy TrEMBL
Match: S0BE08 (GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox3 PE=2 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 6.8e-174
Identity = 296/329 (89.97%), Postives = 309/329 (93.92%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIKSCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQS LVFDHFAPIKSCKRTALFTGIPVI+L DPEAK  IVKACEDFGFFK+VNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVINLKDPEAKIQIVKACEDFGFFKLVNHGVSD 60

Query: 61  DLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           +LI  LE+Q  KFF LPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTN +LIS+
Sbjct: 61  ELIAALESQTLKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIEKSALSRLLKDERSDCCFR 180
           KS+ I  ENPEIFRC VEEYI A+KEMAC+VLE MAEGL IEKSALS+LLKDERSDCCFR
Sbjct: 121 KSISIFHENPEIFRCLVEEYISAMKEMACQVLELMAEGLKIEKSALSKLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPD TSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 240

Query: 241 FIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FI VGDSLQVMTNGRFKSVKHKVLADPIKSRFSM+YFGGPPLNEKIAPLPSLLEEGE+SL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEKSL 300

Query: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIK 330
           YREFTWWDYK+SAYKSRLADCRL PFEIK
Sbjct: 301 YREFTWWDYKSSAYKSRLADCRLTPFEIK 329

[Close]

BLAST of Tan0010065 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 416.8 bits (1070), Expect = 1.7e-116
Identity = 205/331 (61.93%), Postives = 256/331 (77.34%), Query Frame = 0


Query: 1   MVVQSPLVFD-HFAPIKSCKRTALFT--GIPVIDLFDPEAKTHIVKACEDFGFFKVVNHG 60
           +V+  P+  D H + I + K   + T   IPV++L DPEAKT IVKACE+FGFFKVVNHG
Sbjct: 2   VVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHG 61

Query: 61  VSDDLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHEL 120
           V  +L+  LE +A  FF LPQS K +AGPP+P+GYG+KRIGPNGDVGWIEYLLLN N +L
Sbjct: 62  VRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQL 121

Query: 121 ISEKSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIE-KSALSRLLKDERSD 180
            S K+  + R+ P+IFR +VEEY+  +KE++ +VLE +AE LGIE +  LS++L+DE+SD
Sbjct: 122 SSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSD 181

Query: 181 CCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPD 240
            C RLNHYP   E      +  +GFGEHTDPQIISVLRSNNT GL IC++DG+WV+VPPD
Sbjct: 182 SCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPD 241

Query: 241 QTSFFIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEG 300
            +SFFI VGD+LQVMTNGRFKSVKH+VLAD  +SR SM+YFGGPPL++KIAPLP L+ E 
Sbjct: 242 HSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVPEQ 301

Query: 301 EQSLYREFTWWDYKNSAYKSRLADCRLGPFE 328
           +  LY+EFTW  YK+SAYKS+L D RLG FE
Sbjct: 302 DDWLYKEFTWSQYKSSAYKSKLGDYRLGLFE 330

[Close]

BLAST of Tan0010065 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 383.6 bits (984), Expect = 1.6e-106
Identity = 197/340 (57.94%), Postives = 241/340 (70.88%), Query Frame = 0


Query: 1   MVVQSPLVFDHFAPIK-SCKRTALFTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVS 60
           ++V  P  FD    +   CK   +   IPVIDL D +AKT IVKACE+FGFFKV+NHGV 
Sbjct: 2   VIVLQPASFDSNLYVNPKCKPRPVL--IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVR 61

Query: 61  DDLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELIS 120
            DL+  LE +A  FF L  S K+KAGPPDPFGYG+KRIGPNGD+GW+EY+LLN N  L S
Sbjct: 62  PDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLES 121

Query: 121 EKSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIE-KSALSRLLKDERSDCC 180
            K+  I R  P IFR AVEEYI  +K M+ + LE + E L IE K  LSRL+K + SD C
Sbjct: 122 HKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSC 181

Query: 181 FRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQT 240
            R+NHY   PE E    +  IGFGEHTDPQ+IS+LRSN+T GL IC++DG WV V PD +
Sbjct: 182 LRMNHY---PEKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHS 241

Query: 241 SFFIIVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQ 300
           SFF++VGD+LQVMTNGRFKSVKH+V+ +  +SR SM+YF GPPL+EKIAPL  L+ + + 
Sbjct: 242 SFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDD 301

Query: 301 SLYREFTWWDYKNSAYKSRLADCRLGPFEIKKKSHPFLLT 339
            LY EFTW  YK SAYK++L D RLG FE   K  PF L+
Sbjct: 302 CLYNEFTWSQYKLSAYKTKLGDYRLGLFE---KRPPFSLS 333

[Close]

BLAST of Tan0010065 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 371.7 bits (953), Expect = 6.3e-103
Identity = 182/309 (58.90%), Postives = 231/309 (74.76%), Query Frame = 0


Query: 24  FTGIPVIDLFDPEAKTHIVKACEDFGFFKVVNHGVSDDLIGDLEAQAQKFFQLPQSEKEK 83
           F+ IPVID+ DPE+K  +VKACEDFGFFKV+NHGVS +L+  LE +   FF LP+SEK +
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 84  AGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHELISEKSLFILRENPEIFRCAVEEYILA 143
                PFGYG+ +IG NGDVGW+EYLL+N NH+  S      L ++P  FR A+EEY  +
Sbjct: 75  VA-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTS 134

Query: 144 VKEMACEVLEAMAEGLGIE-KSALSRLLKDERSDCCFRLNHYPPCP--ELEALSGRNLIG 203
           V++M  +VLE + +GLGI+ ++ LS+L+ D+ +D   RLNHYPPCP    +   G+N+IG
Sbjct: 135 VRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIG 194

Query: 204 FGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDQTSFFIIVGDSLQVMTNGRFKSVK 263
           FGEHTDPQIISVLRSNNT+GL I L DG+W+SVPPD TSFF  VGDSLQVMTNGRFKSV+
Sbjct: 195 FGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVR 254

Query: 264 HKVLADPIKSRFSMVYFGGPPLNEKIAPLPSLLEEGEQSLYREFTWWDYKNSAYKSRLAD 323
           H+VLA+  KSR SM+YF GP L ++IAPL  L++  ++ LY EFTW +YKNS Y SRL+D
Sbjct: 255 HRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSD 314

Query: 324 CRLGPFEIK 330
            RL  FE K
Sbjct: 315 NRLQQFERK 322

[Close]

BLAST of Tan0010065 vs. TAIR 10
Match: AT1G30040.2 (gibberellin 2-oxidase )

HSP 1 Score: 302.8 bits (774), Expect = 3.6e-82
Identity = 151/254 (59.45%), Postives = 192/254 (75.59%), Query Frame = 0


Query: 1   MVVQSPLVFD-HFAPIKSCKRTALFT--GIPVIDLFDPEAKTHIVKACEDFGFFKVVNHG 60
           +V+  P+  D H + I + K   + T   IPV++L DPEAKT IVKACE+FGFFKVVNHG
Sbjct: 2   VVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHG 61

Query: 61  VSDDLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNHEL 120
           V  +L+  LE +A  FF LPQS K +AGPP+P+GYG+KRIGPNGDVGWIEYLLLN N +L
Sbjct: 62  VRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQL 121

Query: 121 ISEKSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGLGIE-KSALSRLLKDERSD 180
            S K+  + R+ P+IFR +VEEY+  +KE++ +VLE +AE LGIE +  LS++L+DE+SD
Sbjct: 122 SSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSD 181

Query: 181 CCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPD 240
            C RLNHYP   E      +  +GFGEHTDPQIISVLRSNNT GL IC++DG+WV+VPPD
Sbjct: 182 SCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPD 241

Query: 241 QTSFFIIVGDSLQV 251
            +SFFI VGD+LQV
Sbjct: 242 HSSFFINVGDALQV 253

[Close]

BLAST of Tan0010065 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 298.5 bits (763), Expect = 6.7e-81
Identity = 147/289 (50.87%), Postives = 193/289 (66.78%), Query Frame = 0


Query: 41  IVKACEDFGFFKVVNHGVSDDLIGDLEAQAQKFFQLPQSEKEKAGPPDPFGYGSKRIGPN 100
           IVKACE  GFFKV+NHGV  ++I   E + ++FF  P+S+K +AGP  PFGYG K IG N
Sbjct: 41  IVKACEVNGFFKVINHGVKPEIIKRFEHEGEEFFNKPESDKLRAGPASPFGYGCKNIGFN 100

Query: 101 GDVGWIEYLLLNTNHELISEKSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMAEGL- 160
           GD+G +EYLLL+ N   +++KS  I  ++P  F  A  +YI  V+++ACE+++   E L 
Sbjct: 101 GDLGELEYLLLHANPTAVADKSETISHDDPFKFSSATNDYIRTVRDLACEIIDLTIENLW 160

Query: 161 GIEKSALSRLLKDERSDCCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTT 220
           G + S +S L++D RSD   RLNHYPP P   ALSG   IGFGEH+DPQI++VLRSN+  
Sbjct: 161 GQKSSEVSELIRDVRSDSILRLNHYPPAP--YALSGVGQIGFGEHSDPQILTVLRSNDVD 220

Query: 221 GLHICLRDGNWVSVPPDQTSFFIIVGDSLQVMTNGRFKSVKHKVLADPIKS-RFSMVYFG 280
           GL IC RDG W+ +P D T FF++VGD LQ +TNGRF SV+H+VLA+  K  R S +YF 
Sbjct: 221 GLEICSRDGLWIPIPSDPTCFFVLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFA 280

Query: 281 GPPLNEKIAPLPSLLEEGEQSLYREFTWWDYKNSAYKSRLADCRLGPFE 328
            PPL  KI+PLP ++       Y  FTW DYK + Y  RL   RL  F+
Sbjct: 281 APPLEAKISPLPKMVSPENPRRYNSFTWGDYKKATYSLRLDVPRLEFFK 327

The following BLAST results are available for this feature:

BLAST of Tan0010065 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XG83	9.6e-133	70.12	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]
Q9XFR9	2.4e-115	61.93	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
Q9SQ80	5.9e-114	60.67	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
O64692	2.2e-105	57.94	Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ...	[more]
Q8S0S6	3.6e-103	56.06	Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]

BLAST of Tan0010065 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7019518.1	2.8e-182	94.22	Gibberellin 2-beta-dioxygenase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023001443.1	2.8e-182	94.53	gibberellin 2-beta-dioxygenase-like [Cucurbita maxima] >CBL59328.1 Gibberellin 2...	[more]
XP_022927050.1	6.3e-182	94.22	gibberellin 2-beta-dioxygenase-like [Cucurbita moschata]	[more]
XP_023519238.1	8.3e-182	94.53	gibberellin 2-beta-dioxygenase-like [Cucurbita pepo subsp. pepo]	[more]
XP_038893915.1	1.8e-181	94.22	gibberellin 2-beta-dioxygenase-like [Benincasa hispida]	[more]

BLAST of Tan0010065 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
I0J1I9	1.4e-182	94.53	Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox3 PE=2 SV=1	[more]
A0A6J1EMS7	3.1e-182	94.22	gibberellin 2-beta-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC11143399...	[more]
A0A6J1K680	6.0e-178	92.40	gibberellin 2-beta-dioxygenase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1GTD6	2.5e-176	91.79	gibberellin 2-beta-dioxygenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=...	[more]
S0BE08	6.8e-174	89.97	GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox3 PE=2 SV=1	[more]

BLAST of Tan0010065 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30040.1	1.7e-116	61.93	gibberellin 2-oxidase	[more]
AT2G34555.1	1.6e-106	57.94	gibberellin 2-oxidase 3	[more]
AT1G78440.1	6.3e-103	58.90	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]
AT1G30040.2	3.6e-82	59.45	gibberellin 2-oxidase	[more]
AT1G02400.1	6.7e-81	50.87	gibberellin 2-oxidase 6	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 204..225 score: 27.69 coord: 41..58 score: 56.06 coord: 240..266 score: 25.59
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 9..330
None	No IPR available	PANTHER	PTHR47990:SF121	GIBBERELLIN 2-BETA-DIOXYGENASE 2	coord: 9..330
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 14..319
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 27..94 e-value: 2.5E-16 score: 60.5
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 176..278 e-value: 5.6E-25 score: 87.8
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 9..320 e-value: 9.7E-82 score: 276.9
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 174..280 score: 10.490429

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0010065.1	Tan0010065.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045487	gibberellin catabolic process
biological_process	GO:0009416	response to light stimulus
molecular_function	GO:0052634	C-19 gibberellin 2-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding