Tan0009936 (gene) Snake gourd v1

Overview
NameTan0009936
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionnodulation-signaling pathway 2 protein-like
LocationLG03: 69835733 .. 69837274 (-)
RNA-Seq ExpressionTan0009936
SyntenyTan0009936
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACTTGAACCCATTGATTTCCTCACAAATTACACTTACTTTTCATCTGAAACTTTTTTCCAACAAAGCCATGAATTTGCTGAATGCAGCCAGGCAAATTCTTGCTCTTCTCTCTCAGAAAACTCCTCTCAAAATTCCCTCCAAGATGGAAGCTTTTTGGAATATAGTGATCAAAATTTGCTTCAAATGGAGATTTTAGAAGAAGCATTGGATCAGTTTGATCCAATCAATTACAACCCTGAAGATTTCCTCACAAACACCATGGATCATCAAAACATTATTGCAGAAATGGGCAATTCAAGATCAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAAGTCCAATAATATTTTCAGGGGAATTCAAGCAGAGCTAATGGAAGAAGAGAGCTTAACAGATCTGTTACTAGCAGCAGCTGAAGCTGTTGAAGCACAAAACCAACCCCTTGTTTCAAAATTGATTGAAAAGCTAAAGAATTTGCTTTTTTGTGATATGGGTTCATCATCATTCAACCAATTGGCTTGGTTTTTCACTCAAGGCCTGCATTTGAAGACTGTTGATTATGATGTTATTGCAGCTGATCATGAAATGGCAGATCAAGATCAAACAAAGAACAATAACTCCATGTCAGCCTTTCAGATGCTGCAGCAGCTTTCTCCTTACATAAAATTTGCTCATTTCACTGCCAACCAAGCAATACTTGAAGCATCAGAAGGAGAAAAAATGATTCATGTTATAGACTTTGACATCATGGAAGGGATTCAATGGCCACCATTGATGGCTGATCTTGCAGCTAAGAAAGAAGTTTGCTTGTTGAGGCTAACAGCCATTGTACAAGACATTGAAAATGATCAGAGAAAGATTGAACAAACAGGGAGAAGGCTGAGTGAATTTGCCAAATCAATCAATTTCCCTTTCATATTTGATCAAATGGGAATTGAAAAAGAAGAGAACTTTGAGCAAATTCAAGTGATGGGTCAGAGTACAGTAATTGGCAATTGTAGTAGTGGAATTTTGCACCATATTTTAAGCCAAAGAAACCTATCAAAGCTTGAAACTTTCTTAAGTGGGATTGCAAAACTGTCCCCTAAATGTGTGGTTTTAGTTGAAGAAGAGCTTTTTAAGGTCTCTAAGGCTCAATCCATGTCGTTTGTGGAGTTTTTCTTTGAGGCCTTTCACCATTTCTCTGCTCTTTCTGACTCATTTGTGAGATGCTTTTCTGGGGTTTATGAAAATGGGTTGAAACAGGTGATGGAAGAGTTTTTGGGGACAAGAATTTTGGAGTCAGTCAGCCAATTCCCTTGTGATAAAATTAATGCCTTCGCCTTTGATGATCACTTCAAAGGCTACAAGAAAATTCCTTTCAGTTCTTTCAATTGCTCTCAAGCTAAGTATTTGATTAGTCTGTTTAGAGGAGATTTTTGGGTGCAGCATGAGAAGTGCAGCCTAAGTTTATGTTGGAAGTCAAGGCCTTTGTGTACAGCTACAATTTGGGTTCCAAGAATACTAAAAAGTGAACCAAAAATATGA

mRNA sequence

ATGGAACTTGAACCCATTGATTTCCTCACAAATTACACTTACTTTTCATCTGAAACTTTTTTCCAACAAAGCCATGAATTTGCTGAATGCAGCCAGGCAAATTCTTGCTCTTCTCTCTCAGAAAACTCCTCTCAAAATTCCCTCCAAGATGGAAGCTTTTTGGAATATAGTGATCAAAATTTGCTTCAAATGGAGATTTTAGAAGAAGCATTGGATCAGTTTGATCCAATCAATTACAACCCTGAAGATTTCCTCACAAACACCATGGATCATCAAAACATTATTGCAGAAATGGGCAATTCAAGATCAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAAGTCCAATAATATTTTCAGGGGAATTCAAGCAGAGCTAATGGAAGAAGAGAGCTTAACAGATCTGTTACTAGCAGCAGCTGAAGCTGTTGAAGCACAAAACCAACCCCTTGTTTCAAAATTGATTGAAAAGCTAAAGAATTTGCTTTTTTGTGATATGGGTTCATCATCATTCAACCAATTGGCTTGGTTTTTCACTCAAGGCCTGCATTTGAAGACTGTTGATTATGATGTTATTGCAGCTGATCATGAAATGGCAGATCAAGATCAAACAAAGAACAATAACTCCATGTCAGCCTTTCAGATGCTGCAGCAGCTTTCTCCTTACATAAAATTTGCTCATTTCACTGCCAACCAAGCAATACTTGAAGCATCAGAAGGAGAAAAAATGATTCATGTTATAGACTTTGACATCATGGAAGGGATTCAATGGCCACCATTGATGGCTGATCTTGCAGCTAAGAAAGAAGTTTGCTTGTTGAGGCTAACAGCCATTGTACAAGACATTGAAAATGATCAGAGAAAGATTGAACAAACAGGGAGAAGGCTGAGTGAATTTGCCAAATCAATCAATTTCCCTTTCATATTTGATCAAATGGGAATTGAAAAAGAAGAGAACTTTGAGCAAATTCAAGTGATGGGTCAGAGTACAGTAATTGGCAATTGTAGTAGTGGAATTTTGCACCATATTTTAAGCCAAAGAAACCTATCAAAGCTTGAAACTTTCTTAAGTGGGATTGCAAAACTGTCCCCTAAATGTGTGGTTTTAGTTGAAGAAGAGCTTTTTAAGGTCTCTAAGGCTCAATCCATGTCGTTTGTGGAGTTTTTCTTTGAGGCCTTTCACCATTTCTCTGCTCTTTCTGACTCATTTGTGAGATGCTTTTCTGGGGTTTATGAAAATGGGTTGAAACAGGTGATGGAAGAGTTTTTGGGGACAAGAATTTTGGAGTCAGTCAGCCAATTCCCTTGTGATAAAATTAATGCCTTCGCCTTTGATGATCACTTCAAAGGCTACAAGAAAATTCCTTTCAGTTCTTTCAATTGCTCTCAAGCTAAGTATTTGATTAGTCTGTTTAGAGGAGATTTTTGGGTGCAGCATGAGAAGTGCAGCCTAAGTTTATGTTGGAAGTCAAGGCCTTTGTGTACAGCTACAATTTGGGTTCCAAGAATACTAAAAAGTGAACCAAAAATATGA

Coding sequence (CDS)

ATGGAACTTGAACCCATTGATTTCCTCACAAATTACACTTACTTTTCATCTGAAACTTTTTTCCAACAAAGCCATGAATTTGCTGAATGCAGCCAGGCAAATTCTTGCTCTTCTCTCTCAGAAAACTCCTCTCAAAATTCCCTCCAAGATGGAAGCTTTTTGGAATATAGTGATCAAAATTTGCTTCAAATGGAGATTTTAGAAGAAGCATTGGATCAGTTTGATCCAATCAATTACAACCCTGAAGATTTCCTCACAAACACCATGGATCATCAAAACATTATTGCAGAAATGGGCAATTCAAGATCAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAAGTCCAATAATATTTTCAGGGGAATTCAAGCAGAGCTAATGGAAGAAGAGAGCTTAACAGATCTGTTACTAGCAGCAGCTGAAGCTGTTGAAGCACAAAACCAACCCCTTGTTTCAAAATTGATTGAAAAGCTAAAGAATTTGCTTTTTTGTGATATGGGTTCATCATCATTCAACCAATTGGCTTGGTTTTTCACTCAAGGCCTGCATTTGAAGACTGTTGATTATGATGTTATTGCAGCTGATCATGAAATGGCAGATCAAGATCAAACAAAGAACAATAACTCCATGTCAGCCTTTCAGATGCTGCAGCAGCTTTCTCCTTACATAAAATTTGCTCATTTCACTGCCAACCAAGCAATACTTGAAGCATCAGAAGGAGAAAAAATGATTCATGTTATAGACTTTGACATCATGGAAGGGATTCAATGGCCACCATTGATGGCTGATCTTGCAGCTAAGAAAGAAGTTTGCTTGTTGAGGCTAACAGCCATTGTACAAGACATTGAAAATGATCAGAGAAAGATTGAACAAACAGGGAGAAGGCTGAGTGAATTTGCCAAATCAATCAATTTCCCTTTCATATTTGATCAAATGGGAATTGAAAAAGAAGAGAACTTTGAGCAAATTCAAGTGATGGGTCAGAGTACAGTAATTGGCAATTGTAGTAGTGGAATTTTGCACCATATTTTAAGCCAAAGAAACCTATCAAAGCTTGAAACTTTCTTAAGTGGGATTGCAAAACTGTCCCCTAAATGTGTGGTTTTAGTTGAAGAAGAGCTTTTTAAGGTCTCTAAGGCTCAATCCATGTCGTTTGTGGAGTTTTTCTTTGAGGCCTTTCACCATTTCTCTGCTCTTTCTGACTCATTTGTGAGATGCTTTTCTGGGGTTTATGAAAATGGGTTGAAACAGGTGATGGAAGAGTTTTTGGGGACAAGAATTTTGGAGTCAGTCAGCCAATTCCCTTGTGATAAAATTAATGCCTTCGCCTTTGATGATCACTTCAAAGGCTACAAGAAAATTCCTTTCAGTTCTTTCAATTGCTCTCAAGCTAAGTATTTGATTAGTCTGTTTAGAGGAGATTTTTGGGTGCAGCATGAGAAGTGCAGCCTAAGTTTATGTTGGAAGTCAAGGCCTTTGTGTACAGCTACAATTTGGGTTCCAAGAATACTAAAAAGTGAACCAAAAATATGA

Protein sequence

MELEPIDFLTNYTYFSSETFFQQSHEFAECSQANSCSSLSENSSQNSLQDGSFLEYSDQNLLQMEILEEALDQFDPINYNPEDFLTNTMDHQNIIAEMGNSRSEEEEEEEEEEEEEKSNNIFRGIQAELMEEESLTDLLLAAAEAVEAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLAWFFTQGLHLKTVDYDVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKMIHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDIENDQRKIEQTGRRLSEFAKSINFPFIFDQMGIEKEENFEQIQVMGQSTVIGNCSSGILHHILSQRNLSKLETFLSGIAKLSPKCVVLVEEELFKVSKAQSMSFVEFFFEAFHHFSALSDSFVRCFSGVYENGLKQVMEEFLGTRILESVSQFPCDKINAFAFDDHFKGYKKIPFSSFNCSQAKYLISLFRGDFWVQHEKCSLSLCWKSRPLCTATIWVPRILKSEPKI
Homology
BLAST of Tan0009936 vs. ExPASy Swiss-Prot
Match: Q5NE24 (Protein NODULATION SIGNALING PATHWAY 2 OS=Medicago truncatula OX=3880 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 4.1e-42
Identity = 147/470 (31.28%), Postives = 237/470 (50.43%), Query Frame = 0

Query: 74  FDPINYNPEDF--LTNTM--DHQNIIAEMGNS-----RSEEEEEEEEEEEEEKSNNIFRG 133
           +D     P+DF  L +T+  D   ++ ++  S      +    +EEEEE E  +      
Sbjct: 43  WDTFTGAPDDFHHLMDTIIEDRTTVLEQLSPSITTTTTTTTTTDEEEEEMETTTTTTTTA 102

Query: 134 IQAELMEEES----LTDLLLAAAEAV--EAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLA 193
           I+   + ++S    L  LL+A AEA+    +N+ L   ++ +LK L+      S+  +LA
Sbjct: 103 IKTHEVGDDSKGLKLVHLLMAGAEALTGSTKNRDLARVILIRLKELVSQHANGSNMERLA 162

Query: 194 WFFTQGLHLKTVDYDVIAADHEMADQDQ---------TKNNNSMSAFQMLQQLSPYIKFA 253
             FT+ LH           +H   + ++            N++++AFQ+LQ +SPY+KF 
Sbjct: 163 AHFTEALHGLLEGAGGAHNNHHHHNNNKHYLTTNGPHDNQNDTLAAFQLLQDMSPYVKFG 222

Query: 254 HFTANQAILEASEGEKMIHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDIENDQ 313
           HFTANQAI+EA   E+ +HVID+DIMEG+QW  L+  LA+      LR+TA+ +     +
Sbjct: 223 HFTANQAIIEAVAHERRVHVIDYDIMEGVQWASLIQSLASNNNGPHLRITALSRTGTGRR 282

Query: 314 --RKIEQTGRRLSEFAKSINFPFIFDQMGIEKEENF--EQIQVMGQSTVIGNCSSGILHH 373
               +++TGRRL+ FA S+  PF F    ++ +E F    ++++    ++ NC   + H 
Sbjct: 283 SIATVQETGRRLTSFAASLGQPFSFHHCRLDSDETFRPSALKLVRGEALVFNCMLNLPH- 342

Query: 374 ILSQRNLSKLETFLSGIAKLSPKCVVLVEEELFKVSKAQSMSFVEFFFEAFHHFSALSDS 433
            LS R    + +FL+G   L+PK V LVEEE+  V       FVE F ++ HH+SA+ DS
Sbjct: 343 -LSYRAPESVASFLNGAKTLNPKLVTLVEEEVGSVIG----GFVERFMDSLHHYSAVFDS 402

Query: 434 FVRCFSGVYENGLKQVMEE-FLGTRILESVSQFPCDKINAFAFDDHFK---------GYK 493
               F    +N  + ++E  F G RI  S+      +I     ++  +         G++
Sbjct: 403 LEAGFP--MQNRARTLVERVFFGPRIAGSLG-----RIYRTGGEEERRSWGEWLGEVGFR 462

Query: 494 KIPFSSFNCSQAKYLISLFRGDFWVQH---EKCSLSLCWKSRPLCTATIW 503
            +P S  N  QAK L+ LF   + V+        L L WKSR L +A++W
Sbjct: 463 GVPVSFANHCQAKLLLGLFNDGYRVEEVGVGSNKLVLDWKSRRLLSASLW 499

BLAST of Tan0009936 vs. ExPASy Swiss-Prot
Match: Q2PEG7 (Protein NODULATION SIGNALING PATHWAY 2 OS=Lotus japonicus OX=34305 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 7.7e-41
Identity = 143/466 (30.69%), Postives = 237/466 (50.86%), Query Frame = 0

Query: 74  FDPINYNPEDF---LTNTMDHQNIIAEMGNSRSEEEEEEEEEEE---------------- 133
           +D    N +DF   + + +D  N      +  +    EEEEEEE                
Sbjct: 43  WDAFTGNQDDFHHLIDSMIDDNNTGPAFSDHTASTTSEEEEEEEATTTTMTTTTTTTTTT 102

Query: 134 EEKSNNIFRGIQAELMEEESLTDLLLAAAEAVEA--QNQPLVSKLIEKLKNLLFCDMGSS 193
            E +++ F+G++        L  LL+A AEA+    +N+ L   ++ +LK L+    G +
Sbjct: 103 PEAADDDFKGLR--------LVHLLMAGAEALTGANKNRELARVILVRLKELVSHTDG-T 162

Query: 194 SFNQLAWFFTQGLH--LKTVD--YDVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKF 253
           +  +LA +FT+ L   L+     Y+  +  H +        N++++AFQ+LQ +SPY+KF
Sbjct: 163 NMERLAAYFTEALQGLLEGAGGAYNSSSKHHVIGGPHHEPQNDALAAFQLLQDMSPYVKF 222

Query: 254 AHFTANQAILEASEGEKMIHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQD--IE 313
            HFTANQAI+EA   E+ +H++D+DIMEG+QW  LM  LA+      LR+TA+ +     
Sbjct: 223 GHFTANQAIVEAVAHERRVHIVDYDIMEGVQWASLMQALASNPNGPHLRITALSRSGVGR 282

Query: 314 NDQRKIEQTGRRLSEFAKSINFPFIFDQMGIEKEENFEQ--IQVMGQSTVIGNCSSGILH 373
                +++TGRRL+ FA S+  PF F    +E +E F    ++++    ++ NC   + H
Sbjct: 283 RSMATVQETGRRLTAFATSLGQPFSFHHSRLESDETFRPAGLKLVRGEALVFNCMLNLPH 342

Query: 374 HILSQRNLSKLETFLSGIAKLSPKCVVLVEEELFKVSKAQSMSFVEFFFEAFHHFSALSD 433
             L+ R+ + + +FL+    L P+ V +VEEE   V  A    FVE F ++ HHFSA+ D
Sbjct: 343 --LTYRSPNSVASFLTAAKALRPRLVTVVEEE---VGSALG-GFVERFMDSLHHFSAVFD 402

Query: 434 SFVRCFSGVYENGLKQVMEE--FLGTRILESVSQF-----PCDKINAFAFDDHFKGYKKI 493
           S     +G    G  + + E  FLG RI+ S+++        ++  ++       G+  +
Sbjct: 403 SLE---AGFPMQGRARALVERVFLGPRIVGSLARIYRTGGGGEERGSWREWLRAAGFSGV 462

Query: 494 PFSSFNCSQAKYLISLFRGDFWVQH-EKCSLSLCWKSRPLCTATIW 503
             SS N  Q+  L+ LF   + V+      L L WK+R L +A++W
Sbjct: 463 AVSSANHCQSNLLLGLFNDGYRVEELGSNKLVLHWKTRRLLSASLW 490

BLAST of Tan0009936 vs. ExPASy Swiss-Prot
Match: Q84Q92 (Protein NODULATION SIGNALING PATHWAY 2 OS=Oryza sativa subsp. japonica OX=39947 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 8.5e-40
Identity = 127/401 (31.67%), Postives = 205/401 (51.12%), Query Frame = 0

Query: 135 LTDLLLAAAEAVEAQNQP------LVSKLIEKLKNLLFCDMGSSSFNQLAWFFTQGLH-L 194
           L  LL+AAAEA+   ++       ++ +L E + +    +  +S+  +LA  FT  L  L
Sbjct: 132 LLHLLMAAAEALSGPHKSRELARVILVRLKEMVSHTASANAAASNMERLAAHFTDALQGL 191

Query: 195 KTVDYDVIAADHEMADQDQTKN-NNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKM 254
               + V  +  + A      +  + ++AFQMLQ +SPY+KF HFTANQAILEA  G++ 
Sbjct: 192 LDGSHPVGGSGRQAAAAASHHHAGDVLTAFQMLQDMSPYMKFGHFTANQAILEAVSGDRR 251

Query: 255 IHVIDFDIMEGIQWPPLMADLAAKKE---VCLLRLTAIVQDIENDQRKIEQTGRRLSEFA 314
           +H++D+DI EGIQW  LM  + ++ +      LR+TA+ +      R +++ GRRLS FA
Sbjct: 252 VHIVDYDIAEGIQWASLMQAMTSRADGVPAPHLRITAVSRSGGGGARAVQEAGRRLSAFA 311

Query: 315 KSINFPFIFDQMGIEKEENFE--QIQVMGQSTVIGNCSSGILHHILS----QRNLSKLET 374
            SI  PF F Q  ++ +E F    ++++    ++ NC   +LH   +    +R    + +
Sbjct: 312 ASIGQPFSFGQCRLDSDERFRPATVRMVKGEALVANC---VLHQAAATTTIRRPTGSVAS 371

Query: 375 FLSGIAKLSPKCVVLVEEE--------LFKVSKAQSMSFVEFFFEAFHHFSALSDSFVRC 434
           FLSG+A L  K V +VEEE              A +  FV  F E  H +SA+ DS    
Sbjct: 372 FLSGMAALGAKLVTVVEEEGEAEKDDDGDSAGDAAAGGFVRQFMEELHRYSAVWDSLEAG 431

Query: 435 FSGVYENGLKQVMEE-FLGTRILESVSQF--PCDKINAFAFDDHFK--GYKKIPFSSFNC 494
           F    ++ ++ ++E   L   I  +VS+     D      +    +  G+  +P S FN 
Sbjct: 432 FP--TQSRVRGLVERVILAPNIAGAVSRAYRGVDGEGRCGWGQWMRGSGFTAVPLSCFNH 491

Query: 495 SQAKYLISLFRGDFWVQHE-KCSLSLCWKSRPLCTATIWVP 505
           SQA+ L+ LF   + V+      + L WK+R L +A++W P
Sbjct: 492 SQARLLLGLFNDGYTVEETGPNKIVLGWKARRLMSASVWAP 527

BLAST of Tan0009936 vs. ExPASy Swiss-Prot
Match: Q9SUF5 (Scarecrow-like protein 26 OS=Arabidopsis thaliana OX=3702 GN=SCL26 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 7.0e-34
Identity = 122/384 (31.77%), Postives = 194/384 (50.52%), Query Frame = 0

Query: 135 LTDLLLAAAEAVEAQN--QPLVSKLIEKLKNLLFCDMGSSSFNQLAWFFTQGLHLKTVDY 194
           L  LL+AAA+A    N  + L   ++ +LK+L+      ++  +LA  FT GL  K ++ 
Sbjct: 104 LVHLLVAAADASTGANKSRELTRVILARLKDLV-SPGDRTNMERLAAHFTNGLS-KLLER 163

Query: 195 DVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKMIHVIDF 254
           D +    +  D D     + +SAF++LQ +SPY+ F + TA QAILEA + E+ IH++D+
Sbjct: 164 DSVLCPQQHRD-DVYDQADVISAFELLQNMSPYVNFGYLTATQAILEAVKYERRIHIVDY 223

Query: 255 DIMEGIQWPPLMADLAAKK---EVCLLRLTAIVQDIENDQ--RKIEQTGRRLSEFAKSIN 314
           DI EG+QW  LM  L ++        LR+TA+ +     +    +++TGRRL+ FA SI 
Sbjct: 224 DINEGVQWASLMQALVSRNTGPSAQHLRITALSRATNGKKSVAAVQETGRRLTAFADSIG 283

Query: 315 FPFIFDQMGIEKEE-NFEQIQVMGQSTVIGNCSSGILH-HILSQRNLSKLETFLSGIAKL 374
            PF +    ++    +   ++++    V+ NC   +LH    S +  S + +FLS    L
Sbjct: 284 QPFSYQHCKLDTNAFSTSSLKLVRGEAVVINC---MLHLPRFSHQTPSSVISFLSEAKTL 343

Query: 375 SPKCVVLVEEELFKVSKAQSMSFVEFFFEAFHHFSALSDSFVRCFSGVYENGLKQVMEE- 434
           +PK V LV EE   V    +  F+  F +  H FSA+ DS     S    N  +  +E  
Sbjct: 344 NPKLVTLVHEE---VGLMGNQGFLYRFMDLLHQFSAIFDSLEAGLS--IANPARGFVERV 403

Query: 435 FLGTRILESVSQFPCDKINAFAFDD-----HFKGYKKIPFSSFNCSQAKYLISLFRGDFW 494
           F+G  +   +++   +     +F          G+K +  S  N  QAK L+SLF   F 
Sbjct: 404 FIGPWVANWLTRITANDAEVESFASWPQWLETNGFKPLEVSFTNRCQAKLLLSLFNDGFR 463

Query: 495 VQH-EKCSLSLCWKSRPLCTATIW 503
           V+   +  L L WKSR L +A+ W
Sbjct: 464 VEELGQNGLVLGWKSRRLVSASFW 476

BLAST of Tan0009936 vs. ExPASy Swiss-Prot
Match: Q2Z2E9 (Protein SCARECROW OS=Ipomoea nil OX=35883 GN=SCR PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.6e-30
Identity = 127/414 (30.68%), Postives = 211/414 (50.97%), Query Frame = 0

Query: 102 RSEEEEEEEEEEEEEKSNNIFRGIQAELMEEESLTDLLLAAAEAVEAQNQPLVSKLIEKL 161
           R E+EE E+++++EE       G+         L  LLL  AEAV A N    ++++ ++
Sbjct: 399 RREKEELEQQKKDEE-------GLH--------LLTLLLQCAEAVAADNLDEANRMLLQV 458

Query: 162 KNLLFCDMGSSSFNQLAWFFTQGLHLKTVDYDV-IAADHEMADQDQTKNNNSMSAFQMLQ 221
             L       +S  ++A +F++ +  + V+  + I A   +     + N    SAFQ+  
Sbjct: 459 SEL--STPYGTSAQRVAAYFSEAMSARLVNSCLGIYASAPLNALPLSLNQKMASAFQVFN 518

Query: 222 QLSPYIKFAHFTANQAILEASEGEKMIHVIDFDIMEGIQWPPLMADLAAKK-EVCLLRLT 281
            +SP++KF+HFTANQAI EA E E  +H+ID DIM+G+QWP L   LA++     L+RLT
Sbjct: 519 GISPFVKFSHFTANQAIQEAFEREDRVHIIDLDIMQGLQWPGLFHILASRPGGPPLVRLT 578

Query: 282 AIVQDIENDQRKIEQTGRRLSEFAKSINFPFIFDQMGIEKEENF--EQIQVMGQSTVIGN 341
            +   +E     +E TG+RLS+FA+ +  PF F  +  +K  N   +++ V  +  V  +
Sbjct: 579 GLGTSME----ALEATGKRLSDFAQKLGLPFEFFPVA-DKVGNLDPQRLNVNKREAVAVH 638

Query: 342 CSSGILHHILSQRNLSKLETFLSGIAKLSPKCVVLVEEELFKVSKAQSMSFVEFFFEAFH 401
                L H L     S   T L  + +L+PK V +VE++L     + + SF+  F EA H
Sbjct: 639 W----LQHSLYDVTGSDTNT-LWLLQRLAPKVVTVVEQDL-----SHAGSFLGRFVEAIH 698

Query: 402 HFSALSDSFVRCFSGVYENGLKQVMEEFLGTRILESV------SQFPCDKINAFAFDDHF 461
           ++SAL DS   C+    E+  +  +E+ L +R + +V      S+    K N +      
Sbjct: 699 YYSALFDSLGACYG--EESEERHAVEQQLLSREIRNVLAVGGPSRSGEVKFNNWREKFQQ 758

Query: 462 KGYKKIPFSSFNCSQAKYLISLFRGD-FWVQHEKCSLSLCWKSRPLCTATIWVP 505
            G++ +  +    +QA  L+ +F  D + +  +  +L L WK   L TA+ W P
Sbjct: 759 SGFRGVSLAGNAAAQATLLLGMFHSDGYTLAEDNGALKLGWKDLCLLTASAWRP 778

BLAST of Tan0009936 vs. NCBI nr
Match: XP_022962651.1 (nodulation-signaling pathway 2 protein-like [Cucurbita moschata])

HSP 1 Score: 763.8 bits (1971), Expect = 9.2e-217
Identity = 413/508 (81.30%), Postives = 443/508 (87.20%), Query Frame = 0

Query: 1   MELEPIDFLTNYTYFSSETFFQQSHEFAECSQANSCSSLSENSSQNSLQDGSFLEYSDQN 60
           MELEPIDFLTNY+YFS     QQSHEF ECS  NSCSSLSENSSQNSLQDG FLEY+DQN
Sbjct: 4   MELEPIDFLTNYSYFS-----QQSHEFGECSLTNSCSSLSENSSQNSLQDGYFLEYADQN 63

Query: 61  LLQMEILEEALDQFDPINYNPEDFLTNTMDHQNIIAEMGNSRSEEEEEEEEEEEEEKSNN 120
           LLQ E     LDQFDPINYN EDFL  TMDHQ+ I EMG+SRS    +E+EEEEEE S  
Sbjct: 64  LLQQE-----LDQFDPINYNFEDFLATTMDHQS-IPEMGDSRS---VQEDEEEEEENSKT 123

Query: 121 IFRGIQAELMEEESLTDLLLAAAEAVEAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLAWF 180
           +F+GIQAELMEEESLTDLLLAAA+AV+AQN PLVS LI+KL NLL CDMGSSSFNQLAWF
Sbjct: 124 MFKGIQAELMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWF 183

Query: 181 FTQGLHLKTVDYDVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILEA 240
           FTQGLH KTV YD+ A  HEMA+QDQT N NS+SAF MLQQLSPYIKFAHFTANQAILEA
Sbjct: 184 FTQGLHYKTVGYDIAA--HEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEA 243

Query: 241 SEGEKMIHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDIENDQRKIEQTGRRLS 300
           SEGEKMIHVIDFDIMEGIQWPPLMADLAAKK+VCLLRLTAI Q  EN+QR+IE+TGRRLS
Sbjct: 244 SEGEKMIHVIDFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLS 303

Query: 301 EFAKSINFPFIFDQMGIEKEENFEQIQVMGQSTVIGNCSSGILHHILSQRNLSKLETFLS 360
           EFAKSIN PF FD MGIEK+ENFEQIQV+GQ TVI NC SGILH  LS RN SKLETFL+
Sbjct: 304 EFAKSINLPFKFDHMGIEKDENFEQIQVLGQ-TVIANC-SGILHS-LSHRNSSKLETFLN 363

Query: 361 GIAKLSPKCVVLVEEELFKVSKAQSMSFVEFFFEAFHHFSALSDSFVRCFSGVYENGLKQ 420
           G+AKLSPKCVVLVEEELFKVSKAQ MSFVEFFFEAFHHFS+LSDS +RCFSGVYENG KQ
Sbjct: 364 GVAKLSPKCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQ 423

Query: 421 VMEEFLGTRILESVSQFPCDK----INAFAFDDHFKGYKKIPFSSFNCSQAKYLISLFRG 480
           VM+EFLGTRILESVSQFPC+K    +   AF D+FKGYKKIPFSSFNCSQAKYLI+LFRG
Sbjct: 424 VMDEFLGTRILESVSQFPCEKNAMNLREKAF-DYFKGYKKIPFSSFNCSQAKYLITLFRG 483

Query: 481 DFWVQHEKCSLSLCWKSRPLCTATIWVP 505
           DFWVQHE  SLSLCWKSRPLC+ATIWVP
Sbjct: 484 DFWVQHENGSLSLCWKSRPLCSATIWVP 491

BLAST of Tan0009936 vs. NCBI nr
Match: XP_023518115.1 (nodulation-signaling pathway 2 protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 760.8 bits (1963), Expect = 7.8e-216
Identity = 412/508 (81.10%), Postives = 441/508 (86.81%), Query Frame = 0

Query: 1   MELEPIDFLTNYTYFSSETFFQQSHEFAECSQANSCSSLSENSSQNSLQDGSFLEYSDQN 60
           MELEPIDFLTNY+YFS     QQSHEF ECS  NSCSSLSENSSQNSLQDG FLEY+DQN
Sbjct: 4   MELEPIDFLTNYSYFS-----QQSHEFGECSLTNSCSSLSENSSQNSLQDGYFLEYADQN 63

Query: 61  LLQMEILEEALDQFDPINYNPEDFLTNTMDHQNIIAEMGNSRSEEEEEEEEEEEEEKSNN 120
           LLQ E     LDQFDPINYN E+FL  TMDHQN I EMG+SRS    +E+EEEEE  S  
Sbjct: 64  LLQQE-----LDQFDPINYNFEEFLATTMDHQN-IPEMGDSRS---VQEDEEEEEGNSKT 123

Query: 121 IFRGIQAELMEEESLTDLLLAAAEAVEAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLAWF 180
           +F+GIQAELMEEESLTDLLLAAA+AV+AQN PLVS LI+KL NLL CDMGSSSFNQLAWF
Sbjct: 124 MFKGIQAELMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWF 183

Query: 181 FTQGLHLKTVDYDVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILEA 240
           FTQGLH KTV YD+ A  HEMA+QDQT N NS+SAF MLQQLSPYIKFAHFTANQAILEA
Sbjct: 184 FTQGLHYKTVGYDIAA--HEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEA 243

Query: 241 SEGEKMIHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDIENDQRKIEQTGRRLS 300
           SEGEKMIHVIDFDIMEGIQWPPLMADLAAKK+VCLLRLTAI Q  EN+QR+IE+TGRRLS
Sbjct: 244 SEGEKMIHVIDFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLS 303

Query: 301 EFAKSINFPFIFDQMGIEKEENFEQIQVMGQSTVIGNCSSGILHHILSQRNLSKLETFLS 360
           EFAKSIN PF FD MGIEK+ENFEQIQV+GQ TVI NC SGILH  LS RN SKLETFL+
Sbjct: 304 EFAKSINLPFKFDHMGIEKDENFEQIQVLGQ-TVIANC-SGILHS-LSHRNSSKLETFLN 363

Query: 361 GIAKLSPKCVVLVEEELFKVSKAQSMSFVEFFFEAFHHFSALSDSFVRCFSGVYENGLKQ 420
           G+AKLSPKCVVLVEEELFKVSKAQ MSFVEFFFEAFHHFS+LSDS +RCFSGVYENG KQ
Sbjct: 364 GVAKLSPKCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQ 423

Query: 421 VMEEFLGTRILESVSQFPCDK----INAFAFDDHFKGYKKIPFSSFNCSQAKYLISLFRG 480
           VM+EFLGTRILESVSQFPCDK    +   AF D+ KGYKKIPFSSFNCSQAKYLI+LFRG
Sbjct: 424 VMDEFLGTRILESVSQFPCDKNAMNLREKAF-DYLKGYKKIPFSSFNCSQAKYLITLFRG 483

Query: 481 DFWVQHEKCSLSLCWKSRPLCTATIWVP 505
           DFWVQHE  SLSLCWKSRPLC+ATIWVP
Sbjct: 484 DFWVQHENGSLSLCWKSRPLCSATIWVP 491

BLAST of Tan0009936 vs. NCBI nr
Match: KAG6595119.1 (Protein NODULATION SIGNALING PATHWAY 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 758.4 bits (1957), Expect = 3.9e-215
Identity = 410/508 (80.71%), Postives = 442/508 (87.01%), Query Frame = 0

Query: 1   MELEPIDFLTNYTYFSSETFFQQSHEFAECSQANSCSSLSENSSQNSLQDGSFLEYSDQN 60
           MELEPIDFLTNY+YFS     QQSHEF ECS  NSCSSLSENSSQNSLQDG FLEY+DQN
Sbjct: 1   MELEPIDFLTNYSYFS-----QQSHEFGECSLTNSCSSLSENSSQNSLQDGYFLEYADQN 60

Query: 61  LLQMEILEEALDQFDPINYNPEDFLTNTMDHQNIIAEMGNSRSEEEEEEEEEEEEEKSNN 120
           LLQ E     LDQFDPINYN EDFL  TMDHQ+ I EMG+SRS    +E+EEEEEE S  
Sbjct: 61  LLQQE-----LDQFDPINYNFEDFLATTMDHQS-IPEMGDSRS---VQEDEEEEEENSKT 120

Query: 121 IFRGIQAELMEEESLTDLLLAAAEAVEAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLAWF 180
           +F+GIQAELMEEESLTDLLLAAA+AV+AQN PLVS LI+KL NLL CDMGSSSFNQLAWF
Sbjct: 121 MFKGIQAELMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWF 180

Query: 181 FTQGLHLKTVDYDVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILEA 240
           FT+GLH KTV YD+ A  HEMA+QDQT N NS+SAF MLQQLSPYIKFAHFTANQAILEA
Sbjct: 181 FTRGLHYKTVGYDIAA--HEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEA 240

Query: 241 SEGEKMIHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDIENDQRKIEQTGRRLS 300
           SEGEKMIHVIDFDIMEGIQWPPLMADLAAKK+VCLLRLTAI Q  EN+QR+IE+TGRRLS
Sbjct: 241 SEGEKMIHVIDFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLS 300

Query: 301 EFAKSINFPFIFDQMGIEKEENFEQIQVMGQSTVIGNCSSGILHHILSQRNLSKLETFLS 360
           EFAKSIN PF FD MGIEK+ENFEQIQV+GQ+ VI NC SGILH  LS RN SKLETFL+
Sbjct: 301 EFAKSINLPFKFDHMGIEKDENFEQIQVLGQA-VIANC-SGILHS-LSHRNSSKLETFLN 360

Query: 361 GIAKLSPKCVVLVEEELFKVSKAQSMSFVEFFFEAFHHFSALSDSFVRCFSGVYENGLKQ 420
           G+AKLSPKCVVLVEEELFKVSKAQ MSFVEFFFEAFHHFS+LSDS +RCFSGVYENG KQ
Sbjct: 361 GVAKLSPKCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQ 420

Query: 421 VMEEFLGTRILESVSQFPCDK----INAFAFDDHFKGYKKIPFSSFNCSQAKYLISLFRG 480
           VM+EFLGTRILESVSQFPC+K    +   AF D+ KGYKKIPFSSFNCSQAKYLI+LFRG
Sbjct: 421 VMDEFLGTRILESVSQFPCEKNAMNLREKAF-DYLKGYKKIPFSSFNCSQAKYLITLFRG 480

Query: 481 DFWVQHEKCSLSLCWKSRPLCTATIWVP 505
           DFWVQHE  SLSLCWKSRPLC+ATIWVP
Sbjct: 481 DFWVQHENGSLSLCWKSRPLCSATIWVP 488

BLAST of Tan0009936 vs. NCBI nr
Match: KAG7027132.1 (Nodulation-signaling pathway 2 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 758.1 bits (1956), Expect = 5.1e-215
Identity = 410/508 (80.71%), Postives = 442/508 (87.01%), Query Frame = 0

Query: 1   MELEPIDFLTNYTYFSSETFFQQSHEFAECSQANSCSSLSENSSQNSLQDGSFLEYSDQN 60
           MELEPIDFLTNY+YFS     QQSHEF ECS  NSCSSLSENSSQNSLQDG FLEY+DQN
Sbjct: 4   MELEPIDFLTNYSYFS-----QQSHEFGECSLTNSCSSLSENSSQNSLQDGYFLEYADQN 63

Query: 61  LLQMEILEEALDQFDPINYNPEDFLTNTMDHQNIIAEMGNSRSEEEEEEEEEEEEEKSNN 120
           LLQ E     LDQFDPINYN EDFL  TMDHQ+ I EMG+SRS    +E+EEEEEE S  
Sbjct: 64  LLQQE-----LDQFDPINYNFEDFLATTMDHQS-IPEMGDSRS---VQEDEEEEEENSKT 123

Query: 121 IFRGIQAELMEEESLTDLLLAAAEAVEAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLAWF 180
           +F+GIQAELMEEESLTDLLLAAA+AV+AQN PLVS LI+KL NLL CDMGSSSF+QLAWF
Sbjct: 124 MFKGIQAELMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFSQLAWF 183

Query: 181 FTQGLHLKTVDYDVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILEA 240
           FT+GLH KTV YD+ A  HEMA+QDQT N NS+SAF MLQQLSPYIKFAHFTANQAILEA
Sbjct: 184 FTRGLHYKTVGYDIAA--HEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEA 243

Query: 241 SEGEKMIHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDIENDQRKIEQTGRRLS 300
           SEGEKMIHVIDFDIMEGIQWPPLMADLAAKK+VCLLRLTAI Q  EN+QR+IE+TGRRLS
Sbjct: 244 SEGEKMIHVIDFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLS 303

Query: 301 EFAKSINFPFIFDQMGIEKEENFEQIQVMGQSTVIGNCSSGILHHILSQRNLSKLETFLS 360
           EFAKSIN PF FD MGIEK+ENFEQIQV+GQ TVI NC SGILH  LS RN SKLETFL+
Sbjct: 304 EFAKSINLPFKFDHMGIEKDENFEQIQVLGQ-TVIANC-SGILHS-LSHRNSSKLETFLN 363

Query: 361 GIAKLSPKCVVLVEEELFKVSKAQSMSFVEFFFEAFHHFSALSDSFVRCFSGVYENGLKQ 420
           G+AKLSPKCVVLVEEELFKVSKAQ MSFVEFFFEAFHHFS+LSDS +RCFSGVYENG KQ
Sbjct: 364 GVAKLSPKCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQ 423

Query: 421 VMEEFLGTRILESVSQFPCDK----INAFAFDDHFKGYKKIPFSSFNCSQAKYLISLFRG 480
           VM+EFLGTRILESVSQFPC+K    +   AF D+ KGYKKIPFSSFNCSQAKYLI+LFRG
Sbjct: 424 VMDEFLGTRILESVSQFPCEKNAMNLREKAF-DYLKGYKKIPFSSFNCSQAKYLITLFRG 483

Query: 481 DFWVQHEKCSLSLCWKSRPLCTATIWVP 505
           DFWVQHE  SLSLCWKSRPLC+ATIWVP
Sbjct: 484 DFWVQHENGSLSLCWKSRPLCSATIWVP 491

BLAST of Tan0009936 vs. NCBI nr
Match: XP_023003976.1 (nodulation-signaling pathway 2 protein-like [Cucurbita maxima])

HSP 1 Score: 756.9 bits (1953), Expect = 1.1e-214
Identity = 410/508 (80.71%), Postives = 441/508 (86.81%), Query Frame = 0

Query: 1   MELEPIDFLTNYTYFSSETFFQQSHEFAECSQANSCSSLSENSSQNSLQDGSFLEYSDQN 60
           MELEPIDFLTNY+Y     FFQQSHEF ECS  NSCSSLSENSSQNSLQDG FLEY+DQN
Sbjct: 4   MELEPIDFLTNYSY-----FFQQSHEFGECSLTNSCSSLSENSSQNSLQDGYFLEYADQN 63

Query: 61  LLQMEILEEALDQFDPINYNPEDFLTNTMDHQNIIAEMGNSRSEEEEEEEEEEEEEKSNN 120
           LLQ E     LDQFDP NYN EDFLT TMDHQ+ I EMG+SRS    +EE+EEEEE S  
Sbjct: 64  LLQQE-----LDQFDPNNYNFEDFLTTTMDHQS-IPEMGDSRS---VQEEDEEEEENSKT 123

Query: 121 IFRGIQAELMEEESLTDLLLAAAEAVEAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLAWF 180
           +F+GIQAELMEEESLTDLLLAAA+AV+AQN PLVS LI+KL NLL CDMGSSSFNQLAWF
Sbjct: 124 MFKGIQAELMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWF 183

Query: 181 FTQGLHLKTVDYDVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILEA 240
           FTQGLH KTV YD+ A  HEMA+QDQT N NS+SAF MLQQLSPYIKFAHFTANQAILEA
Sbjct: 184 FTQGLHYKTVGYDIAA--HEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEA 243

Query: 241 SEGEKMIHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDIENDQRKIEQTGRRLS 300
           SEGEKMIHVIDFDIMEGIQWPPLM DLAAKK+VCLLRLTAI Q  EN+QR+IE+TGRRLS
Sbjct: 244 SEGEKMIHVIDFDIMEGIQWPPLMEDLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLS 303

Query: 301 EFAKSINFPFIFDQMGIEKEENFEQIQVMGQSTVIGNCSSGILHHILSQRNLSKLETFLS 360
           EFAKSIN PF FD MGIEK+ENFEQIQV+GQ TVIGNC SGILH  LS RN SKLETFL+
Sbjct: 304 EFAKSINLPFKFDHMGIEKDENFEQIQVLGQ-TVIGNC-SGILHS-LSHRNSSKLETFLN 363

Query: 361 GIAKLSPKCVVLVEEELFKVSKAQSMSFVEFFFEAFHHFSALSDSFVRCFSGVYENGLKQ 420
           GI+KLSPKCVVLVEEELFKVSKAQ MSFVEFFFEAFHHFS+LSDS +RCFSGV+ENG KQ
Sbjct: 364 GISKLSPKCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVHENGFKQ 423

Query: 421 VMEEFLGTRILESVSQFPCDK----INAFAFDDHFKGYKKIPFSSFNCSQAKYLISLFRG 480
           VM+EFLGTRILESVSQFPC K    +   AF D+ KGYKKIPFSSFNCSQAKYLI+LFRG
Sbjct: 424 VMDEFLGTRILESVSQFPCGKNAMNLREKAF-DYLKGYKKIPFSSFNCSQAKYLITLFRG 483

Query: 481 DFWVQHEKCSLSLCWKSRPLCTATIWVP 505
           DFWVQHE  +LSLCWKSRPLC+ATIWVP
Sbjct: 484 DFWVQHENGTLSLCWKSRPLCSATIWVP 491

BLAST of Tan0009936 vs. ExPASy TrEMBL
Match: A0A6J1HDU3 (nodulation-signaling pathway 2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111463069 PE=3 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 4.5e-217
Identity = 413/508 (81.30%), Postives = 443/508 (87.20%), Query Frame = 0

Query: 1   MELEPIDFLTNYTYFSSETFFQQSHEFAECSQANSCSSLSENSSQNSLQDGSFLEYSDQN 60
           MELEPIDFLTNY+YFS     QQSHEF ECS  NSCSSLSENSSQNSLQDG FLEY+DQN
Sbjct: 4   MELEPIDFLTNYSYFS-----QQSHEFGECSLTNSCSSLSENSSQNSLQDGYFLEYADQN 63

Query: 61  LLQMEILEEALDQFDPINYNPEDFLTNTMDHQNIIAEMGNSRSEEEEEEEEEEEEEKSNN 120
           LLQ E     LDQFDPINYN EDFL  TMDHQ+ I EMG+SRS    +E+EEEEEE S  
Sbjct: 64  LLQQE-----LDQFDPINYNFEDFLATTMDHQS-IPEMGDSRS---VQEDEEEEEENSKT 123

Query: 121 IFRGIQAELMEEESLTDLLLAAAEAVEAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLAWF 180
           +F+GIQAELMEEESLTDLLLAAA+AV+AQN PLVS LI+KL NLL CDMGSSSFNQLAWF
Sbjct: 124 MFKGIQAELMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWF 183

Query: 181 FTQGLHLKTVDYDVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILEA 240
           FTQGLH KTV YD+ A  HEMA+QDQT N NS+SAF MLQQLSPYIKFAHFTANQAILEA
Sbjct: 184 FTQGLHYKTVGYDIAA--HEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEA 243

Query: 241 SEGEKMIHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDIENDQRKIEQTGRRLS 300
           SEGEKMIHVIDFDIMEGIQWPPLMADLAAKK+VCLLRLTAI Q  EN+QR+IE+TGRRLS
Sbjct: 244 SEGEKMIHVIDFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLS 303

Query: 301 EFAKSINFPFIFDQMGIEKEENFEQIQVMGQSTVIGNCSSGILHHILSQRNLSKLETFLS 360
           EFAKSIN PF FD MGIEK+ENFEQIQV+GQ TVI NC SGILH  LS RN SKLETFL+
Sbjct: 304 EFAKSINLPFKFDHMGIEKDENFEQIQVLGQ-TVIANC-SGILHS-LSHRNSSKLETFLN 363

Query: 361 GIAKLSPKCVVLVEEELFKVSKAQSMSFVEFFFEAFHHFSALSDSFVRCFSGVYENGLKQ 420
           G+AKLSPKCVVLVEEELFKVSKAQ MSFVEFFFEAFHHFS+LSDS +RCFSGVYENG KQ
Sbjct: 364 GVAKLSPKCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQ 423

Query: 421 VMEEFLGTRILESVSQFPCDK----INAFAFDDHFKGYKKIPFSSFNCSQAKYLISLFRG 480
           VM+EFLGTRILESVSQFPC+K    +   AF D+FKGYKKIPFSSFNCSQAKYLI+LFRG
Sbjct: 424 VMDEFLGTRILESVSQFPCEKNAMNLREKAF-DYFKGYKKIPFSSFNCSQAKYLITLFRG 483

Query: 481 DFWVQHEKCSLSLCWKSRPLCTATIWVP 505
           DFWVQHE  SLSLCWKSRPLC+ATIWVP
Sbjct: 484 DFWVQHENGSLSLCWKSRPLCSATIWVP 491

BLAST of Tan0009936 vs. ExPASy TrEMBL
Match: A0A6J1KQT9 (nodulation-signaling pathway 2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111497427 PE=3 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 5.4e-215
Identity = 410/508 (80.71%), Postives = 441/508 (86.81%), Query Frame = 0

Query: 1   MELEPIDFLTNYTYFSSETFFQQSHEFAECSQANSCSSLSENSSQNSLQDGSFLEYSDQN 60
           MELEPIDFLTNY+Y     FFQQSHEF ECS  NSCSSLSENSSQNSLQDG FLEY+DQN
Sbjct: 4   MELEPIDFLTNYSY-----FFQQSHEFGECSLTNSCSSLSENSSQNSLQDGYFLEYADQN 63

Query: 61  LLQMEILEEALDQFDPINYNPEDFLTNTMDHQNIIAEMGNSRSEEEEEEEEEEEEEKSNN 120
           LLQ E     LDQFDP NYN EDFLT TMDHQ+ I EMG+SRS    +EE+EEEEE S  
Sbjct: 64  LLQQE-----LDQFDPNNYNFEDFLTTTMDHQS-IPEMGDSRS---VQEEDEEEEENSKT 123

Query: 121 IFRGIQAELMEEESLTDLLLAAAEAVEAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLAWF 180
           +F+GIQAELMEEESLTDLLLAAA+AV+AQN PLVS LI+KL NLL CDMGSSSFNQLAWF
Sbjct: 124 MFKGIQAELMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWF 183

Query: 181 FTQGLHLKTVDYDVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILEA 240
           FTQGLH KTV YD+ A  HEMA+QDQT N NS+SAF MLQQLSPYIKFAHFTANQAILEA
Sbjct: 184 FTQGLHYKTVGYDIAA--HEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEA 243

Query: 241 SEGEKMIHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDIENDQRKIEQTGRRLS 300
           SEGEKMIHVIDFDIMEGIQWPPLM DLAAKK+VCLLRLTAI Q  EN+QR+IE+TGRRLS
Sbjct: 244 SEGEKMIHVIDFDIMEGIQWPPLMEDLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLS 303

Query: 301 EFAKSINFPFIFDQMGIEKEENFEQIQVMGQSTVIGNCSSGILHHILSQRNLSKLETFLS 360
           EFAKSIN PF FD MGIEK+ENFEQIQV+GQ TVIGNC SGILH  LS RN SKLETFL+
Sbjct: 304 EFAKSINLPFKFDHMGIEKDENFEQIQVLGQ-TVIGNC-SGILHS-LSHRNSSKLETFLN 363

Query: 361 GIAKLSPKCVVLVEEELFKVSKAQSMSFVEFFFEAFHHFSALSDSFVRCFSGVYENGLKQ 420
           GI+KLSPKCVVLVEEELFKVSKAQ MSFVEFFFEAFHHFS+LSDS +RCFSGV+ENG KQ
Sbjct: 364 GISKLSPKCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVHENGFKQ 423

Query: 421 VMEEFLGTRILESVSQFPCDK----INAFAFDDHFKGYKKIPFSSFNCSQAKYLISLFRG 480
           VM+EFLGTRILESVSQFPC K    +   AF D+ KGYKKIPFSSFNCSQAKYLI+LFRG
Sbjct: 424 VMDEFLGTRILESVSQFPCGKNAMNLREKAF-DYLKGYKKIPFSSFNCSQAKYLITLFRG 483

Query: 481 DFWVQHEKCSLSLCWKSRPLCTATIWVP 505
           DFWVQHE  +LSLCWKSRPLC+ATIWVP
Sbjct: 484 DFWVQHENGTLSLCWKSRPLCSATIWVP 491

BLAST of Tan0009936 vs. ExPASy TrEMBL
Match: A0A0A0KKU8 (GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G484540 PE=3 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 4.6e-198
Identity = 378/510 (74.12%), Postives = 418/510 (81.96%), Query Frame = 0

Query: 6   IDFLTNYTYFSSETFFQQSHEFAECSQANSCSSLSENSSQNSLQDGSFLEYSDQNLLQME 65
           ++F TNY YFSS   F  +HE       NSCSSLSENSSQNS Q+GSFLEY DQN L   
Sbjct: 1   MEFFTNYNYFSSPETFFHTHE------GNSCSSLSENSSQNSFQEGSFLEYDDQNFL--- 60

Query: 66  ILEEALDQFDPINYNPEDFLTNT-MDHQNIIAEMGNSRSEEEEEEEEEEEEEKSNNIFRG 125
                  QFDPI+YNPEDFL  T +D QN I E  N     EEE+ EEE+E+ +N IF+G
Sbjct: 61  -------QFDPIDYNPEDFLAITSIDPQN-IQESSNHSGHVEEEDHEEEQEKSNNIIFKG 120

Query: 126 IQAELMEEESLTDLLLAAAEAVEAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLAWFFTQG 185
           IQAELMEEESLTDLLLAAAEA+EAQN  LVS LIEKLKNLL  DMGSSSFNQLAWFFTQG
Sbjct: 121 IQAELMEEESLTDLLLAAAEAIEAQNHVLVSNLIEKLKNLLLYDMGSSSFNQLAWFFTQG 180

Query: 186 LHLKTVDYDVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGE 245
           LH KTVDY+++AA H++    Q KN NSMSAFQMLQQLSPYIKFAHFTANQAILEA+EGE
Sbjct: 181 LHYKTVDYNILAAHHQI----QIKNYNSMSAFQMLQQLSPYIKFAHFTANQAILEAAEGE 240

Query: 246 KMIHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDIENDQRKIEQTGRRLSEFAK 305
           KMIH+IDFDIMEGIQWPPLMADLAAK+ VC LRLTAIVQD EN+++ IEQTGRRLSEFAK
Sbjct: 241 KMIHIIDFDIMEGIQWPPLMADLAAKEHVCSLRLTAIVQDNENERKIIEQTGRRLSEFAK 300

Query: 306 SINFPFIFDQMGIEKEENFEQIQVMGQSTVIGNCSSGILHHILSQRNLSKLETFLSGIAK 365
           SIN PFIFDQMGIEK + FE+IQVMG+ TVIGNC SGI HHILS  NLSK E FL G++K
Sbjct: 301 SINLPFIFDQMGIEKADRFEEIQVMGE-TVIGNC-SGIFHHILSYENLSKFEIFLDGVSK 360

Query: 366 LSPKCVVLVEEELFKVSK-------AQSMSFVEFFFEAFHHFSALSDSFVRCFSGVYENG 425
           LSPKCVVLVEEELF V+K         +MSFVEFFFEAFHHFSALSDS +RCFSGVYENG
Sbjct: 361 LSPKCVVLVEEELFNVTKQLGLGGPQPTMSFVEFFFEAFHHFSALSDSLLRCFSGVYENG 420

Query: 426 LKQVMEEFLGTRILESVSQFPCDKINAFAFD-DHFKGYKKIPFSSFNCSQAKYLISLFRG 485
            KQVM+EFLGTRIL+SV+QFPCDK + +    DH +GY KIPF+SFNCSQAKYLISLFRG
Sbjct: 421 FKQVMDEFLGTRILDSVTQFPCDKTHVWGSGFDHLQGYNKIPFTSFNCSQAKYLISLFRG 480

Query: 486 DFWVQHEKCSLSLCWKSRPLCTATIWVPRI 507
           DFWVQHEKC+LSLCWKSRPLCTATIWVP +
Sbjct: 481 DFWVQHEKCNLSLCWKSRPLCTATIWVPSV 487

BLAST of Tan0009936 vs. ExPASy TrEMBL
Match: A0A5D3DPX1 (Nodulation-signaling pathway 2 protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1230G00340 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 8.2e-195
Identity = 375/514 (72.96%), Postives = 417/514 (81.13%), Query Frame = 0

Query: 1   MELEPIDFLTNYTYFSSETFFQQSHEFAECSQANSCSSLSENSSQNSLQDGSFLEYSDQN 60
           ME E ++F TNY YFSS   F  +HE       NSCSSLSENSSQNS Q+GSFLEY DQ+
Sbjct: 4   MEFESMEFFTNYNYFSSPEAFFHTHE------GNSCSSLSENSSQNSFQEGSFLEYDDQS 63

Query: 61  LLQMEILEEALDQFDPINYNPEDFLTNT-MDHQNIIAEMGNSRSEEEEEEEEEEEEEKSN 120
            L          QFDPINYNPED L  T +D QN   E+ +     +EE+ EEE++E +N
Sbjct: 64  FL----------QFDPINYNPEDLLAVTSIDPQN-NQELSDHSGHVDEEDHEEEQDESNN 123

Query: 121 NIFRGIQAELMEEESLTDLLLAAAEAVEAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLAW 180
            IF+GIQAELMEEESLTDLLLAAAEA+EAQN  LVS LIEKLKNLL  DMGSSSFNQLAW
Sbjct: 124 IIFKGIQAELMEEESLTDLLLAAAEAIEAQNHLLVSNLIEKLKNLLLYDMGSSSFNQLAW 183

Query: 181 FFTQGLHLKTVDYDVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILE 240
           FFTQGLH KTVD +++AA H++    Q  N NSMSAFQMLQQLSPYIKFAHFTANQAILE
Sbjct: 184 FFTQGLHYKTVDNNILAAHHQI----QINNYNSMSAFQMLQQLSPYIKFAHFTANQAILE 243

Query: 241 ASEGEKMIHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDIENDQRKIEQTGRRL 300
           A+EGE+MIH+IDFDIMEGIQWPPLMADLAAK+ VC LRLTAIVQD EN+++ IEQTGRRL
Sbjct: 244 AAEGERMIHIIDFDIMEGIQWPPLMADLAAKEHVCSLRLTAIVQDNENERKIIEQTGRRL 303

Query: 301 SEFAKSINFPFIFDQMGIEKEENFEQIQVMGQSTVIGNCSSGILHHILSQRNLSKLETFL 360
           SEFAKS+N PFIFDQ+GIEK + FE+IQVMG+ TVI NC SGI HHILS  NLSK  TFL
Sbjct: 304 SEFAKSVNLPFIFDQIGIEKADKFEEIQVMGE-TVIANC-SGIFHHILSYENLSKFGTFL 363

Query: 361 SGIAKLSPKCVVLVEEELFKVSKAQS-------MSFVEFFFEAFHHFSALSDSFVRCFSG 420
            G++KLSPKCVVLVEEELF V+K          MSFVEFFFEAFHHFSALSDS +RCFSG
Sbjct: 364 DGVSKLSPKCVVLVEEELFNVTKQLGLGGPQPIMSFVEFFFEAFHHFSALSDSLLRCFSG 423

Query: 421 VYENGLKQVMEEFLGTRILESVSQFPCDK--INAFAFDDHFKGYKKIPFSSFNCSQAKYL 480
           VYENG KQVM+EFLGTRIL+SV+QFPCDK  ++  +  DH KGYKKIPFSSFNCSQAKYL
Sbjct: 424 VYENGFKQVMDEFLGTRILDSVTQFPCDKTHVSWGSGFDHLKGYKKIPFSSFNCSQAKYL 483

Query: 481 ISLFRGDFWVQHEKCSLSLCWKSRPLCTATIWVP 505
           ISLFRGDFWVQHEKCSLSLCWKSRPLCTATIWVP
Sbjct: 484 ISLFRGDFWVQHEKCSLSLCWKSRPLCTATIWVP 494

BLAST of Tan0009936 vs. ExPASy TrEMBL
Match: A0A1S3B384 (nodulation-signaling pathway 2 protein-like OS=Cucumis melo OX=3656 GN=LOC103485258 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 8.2e-195
Identity = 375/514 (72.96%), Postives = 417/514 (81.13%), Query Frame = 0

Query: 1   MELEPIDFLTNYTYFSSETFFQQSHEFAECSQANSCSSLSENSSQNSLQDGSFLEYSDQN 60
           ME E ++F TNY YFSS   F  +HE       NSCSSLSENSSQNS Q+GSFLEY DQ+
Sbjct: 4   MEFESMEFFTNYNYFSSPEAFFHTHE------GNSCSSLSENSSQNSFQEGSFLEYDDQS 63

Query: 61  LLQMEILEEALDQFDPINYNPEDFLTNT-MDHQNIIAEMGNSRSEEEEEEEEEEEEEKSN 120
            L          QFDPINYNPED L  T +D QN   E+ +     +EE+ EEE++E +N
Sbjct: 64  FL----------QFDPINYNPEDLLAVTSIDPQN-NQELSDHSGHVDEEDHEEEQDESNN 123

Query: 121 NIFRGIQAELMEEESLTDLLLAAAEAVEAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLAW 180
            IF+GIQAELMEEESLTDLLLAAAEA+EAQN  LVS LIEKLKNLL  DMGSSSFNQLAW
Sbjct: 124 IIFKGIQAELMEEESLTDLLLAAAEAIEAQNHLLVSNLIEKLKNLLLYDMGSSSFNQLAW 183

Query: 181 FFTQGLHLKTVDYDVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILE 240
           FFTQGLH KTVD +++AA H++    Q  N NSMSAFQMLQQLSPYIKFAHFTANQAILE
Sbjct: 184 FFTQGLHYKTVDNNILAAHHQI----QINNYNSMSAFQMLQQLSPYIKFAHFTANQAILE 243

Query: 241 ASEGEKMIHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDIENDQRKIEQTGRRL 300
           A+EGE+MIH+IDFDIMEGIQWPPLMADLAAK+ VC LRLTAIVQD EN+++ IEQTGRRL
Sbjct: 244 AAEGERMIHIIDFDIMEGIQWPPLMADLAAKEHVCSLRLTAIVQDNENERKIIEQTGRRL 303

Query: 301 SEFAKSINFPFIFDQMGIEKEENFEQIQVMGQSTVIGNCSSGILHHILSQRNLSKLETFL 360
           SEFAKS+N PFIFDQ+GIEK + FE+IQVMG+ TVI NC SGI HHILS  NLSK  TFL
Sbjct: 304 SEFAKSVNLPFIFDQIGIEKADKFEEIQVMGE-TVIANC-SGIFHHILSYENLSKFGTFL 363

Query: 361 SGIAKLSPKCVVLVEEELFKVSKAQS-------MSFVEFFFEAFHHFSALSDSFVRCFSG 420
            G++KLSPKCVVLVEEELF V+K          MSFVEFFFEAFHHFSALSDS +RCFSG
Sbjct: 364 DGVSKLSPKCVVLVEEELFNVTKQLGLGGPQPIMSFVEFFFEAFHHFSALSDSLLRCFSG 423

Query: 421 VYENGLKQVMEEFLGTRILESVSQFPCDK--INAFAFDDHFKGYKKIPFSSFNCSQAKYL 480
           VYENG KQVM+EFLGTRIL+SV+QFPCDK  ++  +  DH KGYKKIPFSSFNCSQAKYL
Sbjct: 424 VYENGFKQVMDEFLGTRILDSVTQFPCDKTHVSWGSGFDHLKGYKKIPFSSFNCSQAKYL 483

Query: 481 ISLFRGDFWVQHEKCSLSLCWKSRPLCTATIWVP 505
           ISLFRGDFWVQHEKCSLSLCWKSRPLCTATIWVP
Sbjct: 484 ISLFRGDFWVQHEKCSLSLCWKSRPLCTATIWVP 494

BLAST of Tan0009936 vs. TAIR 10
Match: AT4G08250.1 (GRAS family transcription factor )

HSP 1 Score: 146.7 bits (369), Expect = 5.0e-35
Identity = 122/384 (31.77%), Postives = 194/384 (50.52%), Query Frame = 0

Query: 135 LTDLLLAAAEAVEAQN--QPLVSKLIEKLKNLLFCDMGSSSFNQLAWFFTQGLHLKTVDY 194
           L  LL+AAA+A    N  + L   ++ +LK+L+      ++  +LA  FT GL  K ++ 
Sbjct: 104 LVHLLVAAADASTGANKSRELTRVILARLKDLV-SPGDRTNMERLAAHFTNGLS-KLLER 163

Query: 195 DVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKMIHVIDF 254
           D +    +  D D     + +SAF++LQ +SPY+ F + TA QAILEA + E+ IH++D+
Sbjct: 164 DSVLCPQQHRD-DVYDQADVISAFELLQNMSPYVNFGYLTATQAILEAVKYERRIHIVDY 223

Query: 255 DIMEGIQWPPLMADLAAKK---EVCLLRLTAIVQDIENDQ--RKIEQTGRRLSEFAKSIN 314
           DI EG+QW  LM  L ++        LR+TA+ +     +    +++TGRRL+ FA SI 
Sbjct: 224 DINEGVQWASLMQALVSRNTGPSAQHLRITALSRATNGKKSVAAVQETGRRLTAFADSIG 283

Query: 315 FPFIFDQMGIEKEE-NFEQIQVMGQSTVIGNCSSGILH-HILSQRNLSKLETFLSGIAKL 374
            PF +    ++    +   ++++    V+ NC   +LH    S +  S + +FLS    L
Sbjct: 284 QPFSYQHCKLDTNAFSTSSLKLVRGEAVVINC---MLHLPRFSHQTPSSVISFLSEAKTL 343

Query: 375 SPKCVVLVEEELFKVSKAQSMSFVEFFFEAFHHFSALSDSFVRCFSGVYENGLKQVMEE- 434
           +PK V LV EE   V    +  F+  F +  H FSA+ DS     S    N  +  +E  
Sbjct: 344 NPKLVTLVHEE---VGLMGNQGFLYRFMDLLHQFSAIFDSLEAGLS--IANPARGFVERV 403

Query: 435 FLGTRILESVSQFPCDKINAFAFDD-----HFKGYKKIPFSSFNCSQAKYLISLFRGDFW 494
           F+G  +   +++   +     +F          G+K +  S  N  QAK L+SLF   F 
Sbjct: 404 FIGPWVANWLTRITANDAEVESFASWPQWLETNGFKPLEVSFTNRCQAKLLLSLFNDGFR 463

Query: 495 VQH-EKCSLSLCWKSRPLCTATIW 503
           V+   +  L L WKSR L +A+ W
Sbjct: 464 VEELGQNGLVLGWKSRRLVSASFW 476

BLAST of Tan0009936 vs. TAIR 10
Match: AT3G54220.1 (GRAS family transcription factor )

HSP 1 Score: 131.0 bits (328), Expect = 2.8e-30
Identity = 133/436 (30.50%), Postives = 212/436 (48.62%), Query Frame = 0

Query: 81  PEDFLTNTMDHQNIIAEMGNSRSEEEEEEEEEEEEEKSNNIFRGIQAELMEEESLTDLLL 140
           PE         Q   AE    R EE + ++++EE         G+         L  LLL
Sbjct: 253 PETVTATVPAVQTNTAEALRERKEEIKRQKQDEE---------GLH--------LLTLLL 312

Query: 141 AAAEAVEAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLAWFFTQGLHLKTVDYDV-IAADH 200
             AEAV A N    +KL+ ++  L       +S  ++A +F++ +  + ++  + I A  
Sbjct: 313 QCAEAVSADNLEEANKLLLEISQL--STPYGTSAQRVAAYFSEAMSARLLNSCLGIYAAL 372

Query: 201 EMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKMIHVIDFDIMEGIQ 260
                 QT +   +SAFQ+   +SP +KF+HFTANQAI EA E E  +H+ID DIM+G+Q
Sbjct: 373 PSRWMPQTHSLKMVSAFQVFNGISPLVKFSHFTANQAIQEAFEKEDSVHIIDLDIMQGLQ 432

Query: 261 WPPLMADLAAKK-EVCLLRLTAIVQDIENDQRKIEQTGRRLSEFAKSINFPFIFDQMGIE 320
           WP L   LA++      +RLT +   +E     ++ TG+RLS+FA  +  PF F  +  E
Sbjct: 433 WPGLFHILASRPGGPPHVRLTGLGTSME----ALQATGKRLSDFADKLGLPFEFCPLA-E 492

Query: 321 KEENF--EQIQVMGQSTVIGNCSSGILHHILSQRNLSKLETFLSGIAKLSPKCVVLVEEE 380
           K  N   E++ V  +  V  +     L H L     S   T L  + +L+PK V +VE++
Sbjct: 493 KVGNLDTERLNVRKREAVAVHW----LQHSLYDVTGSDAHT-LWLLQRLAPKVVTVVEQD 552

Query: 381 LFKVSKAQSMSFVEFFFEAFHHFSALSDSFVRCFSGVYENGLKQVMEEFLGTRILESV-- 440
           L     + + SF+  F EA H++SAL DS    +    E+  + V+E+ L ++ + +V  
Sbjct: 553 L-----SHAGSFLGRFVEAIHYYSALFDSLGASYG--EESEERHVVEQQLLSKEIRNVLA 612

Query: 441 ----SQFPCDKINAFAFDDHFKGYKKIPFSSFNCSQAKYLISLFRGD-FWVQHEKCSLSL 500
               S+    K  ++       G+K I  +    +QA  L+ +F  D + +  +  +L L
Sbjct: 613 VGGPSRSGEVKFESWREKMQQCGFKGISLAGNAATQATLLLGMFPSDGYTLVDDNGTLKL 652

Query: 501 CWKSRPLCTATIWVPR 506
            WK   L TA+ W PR
Sbjct: 673 GWKDLSLLTASAWTPR 652

BLAST of Tan0009936 vs. TAIR 10
Match: AT5G41920.1 (GRAS family transcription factor )

HSP 1 Score: 126.3 bits (316), Expect = 6.9e-29
Identity = 112/386 (29.02%), Postives = 187/386 (48.45%), Query Frame = 0

Query: 135 LTDLLLAAAEAVEAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLAWFFTQGLHLKTVDYDV 194
           L  LLL  AE V   +    S L+ ++  +  C    SS  ++  +F Q L  + +   +
Sbjct: 40  LLSLLLQCAEYVATDHLREASTLLSEISEI--CSPFGSSPERVVAYFAQALQTRVISSYL 99

Query: 195 IAADHEMADQDQT--KNNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKMIHVIDF 254
             A   ++++  T  ++    SA Q    +SP IKF+HFTANQAI +A +GE  +H+ID 
Sbjct: 100 SGACSPLSEKPLTVVQSQKIFSALQTYNSVSPLIKFSHFTANQAIFQALDGEDSVHIIDL 159

Query: 255 DIMEGIQWPPLMADLAAK-KEVCLLRLTAIVQDIENDQRKIEQTGRRLSEFAKSINFPFI 314
           D+M+G+QWP L   LA++ +++  +R+T       +    +  TGRRL++FA S+N PF 
Sbjct: 160 DVMQGLQWPALFHILASRPRKLRSIRITG----FGSSSDLLASTGRRLADFASSLNLPFE 219

Query: 315 FDQMGIEKEENFEQIQV---MGQSTVIGNCSSGILHHILSQRNLSKLETFLSGIAKLSPK 374
           F  +        +  Q+    G++ V+       + H L     + LET L  + +L P 
Sbjct: 220 FHPIEGIIGNLIDPSQLATRQGEAVVV-----HWMQHRLYDVTGNNLET-LEILRRLKPN 279

Query: 375 CVVLVEEELFKVSKAQSMSFVEFFFEAFHHFSALSDSFVRCFSGVYENGLKQVMEEF-LG 434
            + +VE+EL   S     SF+  F EA H++SAL D+         E+G +  +E+  LG
Sbjct: 280 LITVVEQEL---SYDDGGSFLGRFVEALHYYSALFDALGDGLG--EESGERFTVEQIVLG 339

Query: 435 TRILESVSQFPCDKINAFAFDDHFK-GYKKIPFSSFNCSQAKYLISLFR-GDFWVQHEKC 494
           T I   V+     +      ++  + G++ +       +QA  L+ +     + +  E  
Sbjct: 340 TEIRNIVAHGGGRRKRMKWKEELSRVGFRPVSLRGNPATQAGLLLGMLPWNGYTLVEENG 399

Query: 495 SLSLCWKSRPLCTATIWVPRILKSEP 512
           +L L WK   L TA+ W     KS+P
Sbjct: 400 TLRLGWKDLSLLTASAW-----KSQP 403

BLAST of Tan0009936 vs. TAIR 10
Match: AT3G03450.1 (RGA-like 2 )

HSP 1 Score: 123.6 bits (309), Expect = 4.5e-28
Identity = 130/483 (26.92%), Postives = 219/483 (45.34%), Query Frame = 0

Query: 75  DPINYNPEDFLTNTMDHQNIIAEMGNSRSEE------------------------EEEEE 134
           D ++YNP D L+N +  +++++E+ N  S +                         +EEE
Sbjct: 85  DSVHYNPSD-LSNWV--ESMLSELNNPASSDLDTTRSCVDRSEYDLRAIPGLSAFPKEEE 144

Query: 135 EEEEEEKSNNIFRGIQAELMEEES--------------LTDLLLAAAEAVEAQNQPLVSK 194
             +EE  S  I  G   E  +E +              L   L+A AEA+  +N  L   
Sbjct: 145 VFDEEASSKRIRLGSWCESSDESTRSVVLVDSQETGVRLVHALVACAEAIHQENLNLADA 204

Query: 195 LIEKLKNLLFCDMGSSSFNQLAWFFTQGLHLK-----TVDYDVIAADHEMADQDQTKNNN 254
           L++++  L     G  +  ++A +F Q L  +     T + DV AA           N +
Sbjct: 205 LVKRVGTLAGSQAG--AMGKVATYFAQALARRIYRDYTAETDVCAA----------VNPS 264

Query: 255 SMSAFQM-LQQLSPYIKFAHFTANQAILEASEGEKMIHVIDFDIMEGIQWPPLMADLAAK 314
                +M   +  PY+KFAHFTANQAILEA    + +HVID  + +G+QWP LM  LA +
Sbjct: 265 FEEVLEMHFYESCPYLKFAHFTANQAILEAVTTARRVHVIDLGLNQGMQWPALMQALALR 324

Query: 315 K-EVCLLRLTAIVQDIENDQRKIEQTGRRLSEFAKSINFPFIFDQMGIEKEENF--EQIQ 374
                  RLT I      +   ++Q G +L++FA+++   F F  +  E   +   E  +
Sbjct: 325 PGGPPSFRLTGIGPPQTENSDSLQQLGWKLAQFAQNMGVEFEFKGLAAESLSDLEPEMFE 384

Query: 375 VMGQSTVIGNCSSGILHHILSQRNLSKLETFLSGIAKLSPKCVVLVEEELFKVSKAQSMS 434
              +S  +   S   LH +L++     +E  L+ +  + P  V +VE+E    +    + 
Sbjct: 385 TRPESETLVVNSVFELHRLLARS--GSIEKLLNTVKAIKPSIVTVVEQE----ANHNGIV 444

Query: 435 FVEFFFEAFHHFSALSDSFVRCFSGVYENGLKQVMEEFLGTRILESVSQFPCDKINAFAF 494
           F++ F EA H++S+L DS    +S   ++  + + E +LG +IL  V+    D++     
Sbjct: 445 FLDRFNEALHYYSSLFDSLEDSYSLPSQD--RVMSEVYLGRQILNVVAAEGSDRVERHET 504

Query: 495 DDHFK------GYKKIPFSSFNCSQAKYLISLF-RGD-FWVQHEKCSLSLCWKSRPLCTA 503
              ++      G+  I   S    QA  L+SL+  GD + V+     L + W++RPL T 
Sbjct: 505 AAQWRIRMKSAGFDPIHLGSSAFKQASMLLSLYATGDGYRVEENDGCLMIGWQTRPLITT 544

BLAST of Tan0009936 vs. TAIR 10
Match: AT5G17490.1 (RGA-like protein 3 )

HSP 1 Score: 122.9 bits (307), Expect = 7.7e-28
Identity = 112/388 (28.87%), Postives = 187/388 (48.20%), Query Frame = 0

Query: 129 LMEEES--LTDLLLAAAEAVEAQNQPLVSKLIEKLKNLLFCDMGSSSFNQLAWFFTQGLH 188
           L+EE    L   L+A AEAV+ +N  L   L++++  L     G  +  ++A +F + L 
Sbjct: 149 LIEETGVRLVQALVACAEAVQLENLSLADALVKRVGLLAASQAG--AMGKVATYFAEALA 208

Query: 189 LKTVDYDVIAADHEMADQDQTKNNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKM 248
            +       AA  + + ++  + N             PY+KFAHFTANQAILEA    ++
Sbjct: 209 RRIYRIHPSAAAIDPSFEEILQMN--------FYDSCPYLKFAHFTANQAILEAVTTSRV 268

Query: 249 IHVIDFDIMEGIQWPPLMADLAAKK-EVCLLRLTAIVQDIENDQRKIEQTGRRLSEFAKS 308
           +HVID  + +G+QWP LM  LA +       RLT +     +++  I++ G +L++ A++
Sbjct: 269 VHVIDLGLNQGMQWPALMQALALRPGGPPSFRLTGVGN--PSNREGIQELGWKLAQLAQA 328

Query: 309 INFPFIFDQMGIEKEENFE--QIQVMGQSTVIGNCSSGILHHILSQRNLSKLETFLSGIA 368
           I   F F+ +  E+  + E    +   +S  +   S   LH +LSQ     +E  L+ + 
Sbjct: 329 IGVEFKFNGLTTERLSDLEPDMFETRTESETLVVNSVFELHPVLSQP--GSIEKLLATVK 388

Query: 369 KLSPKCVVLVEEELFKVSKAQSMSFVEFFFEAFHHFSALSDSFVRCFSGVYENGLKQVME 428
            + P  V +VE+E    +      F++ F EA H++S+L DS      GV      +VM 
Sbjct: 389 AVKPGLVTVVEQE----ANHNGDVFLDRFNEALHYYSSLFDSLE---DGVVIPSQDRVMS 448

Query: 429 E-FLGTRILESVSQFPCDKINAFAFDDHFK------GYKKIPFSSFNCSQAKYLISLFRG 488
           E +LG +IL  V+    D+I        ++      G+  +   S    QA  L++L  G
Sbjct: 449 EVYLGRQILNLVATEGSDRIERHETLAQWRKRMGSAGFDPVNLGSDAFKQASLLLALSGG 508

Query: 489 --DFWVQHEKCSLSLCWKSRPLCTATIW 503
              + V+    SL L W+++PL  A+ W
Sbjct: 509 GDGYRVEENDGSLMLAWQTKPLIAASAW 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5NE244.1e-4231.28Protein NODULATION SIGNALING PATHWAY 2 OS=Medicago truncatula OX=3880 GN=NSP2 PE... [more]
Q2PEG77.7e-4130.69Protein NODULATION SIGNALING PATHWAY 2 OS=Lotus japonicus OX=34305 GN=NSP2 PE=1 ... [more]
Q84Q928.5e-4031.67Protein NODULATION SIGNALING PATHWAY 2 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9SUF57.0e-3431.77Scarecrow-like protein 26 OS=Arabidopsis thaliana OX=3702 GN=SCL26 PE=2 SV=1[more]
Q2Z2E91.6e-3030.68Protein SCARECROW OS=Ipomoea nil OX=35883 GN=SCR PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022962651.19.2e-21781.30nodulation-signaling pathway 2 protein-like [Cucurbita moschata][more]
XP_023518115.17.8e-21681.10nodulation-signaling pathway 2 protein-like [Cucurbita pepo subsp. pepo][more]
KAG6595119.13.9e-21580.71Protein NODULATION SIGNALING PATHWAY 2, partial [Cucurbita argyrosperma subsp. s... [more]
KAG7027132.15.1e-21580.71Nodulation-signaling pathway 2 protein, partial [Cucurbita argyrosperma subsp. a... [more]
XP_023003976.11.1e-21480.71nodulation-signaling pathway 2 protein-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1HDU34.5e-21781.30nodulation-signaling pathway 2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1KQT95.4e-21580.71nodulation-signaling pathway 2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A0A0KKU84.6e-19874.12GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G484540 PE=3 S... [more]
A0A5D3DPX18.2e-19572.96Nodulation-signaling pathway 2 protein-like OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3B3848.2e-19572.96nodulation-signaling pathway 2 protein-like OS=Cucumis melo OX=3656 GN=LOC103485... [more]
Match NameE-valueIdentityDescription
AT4G08250.15.0e-3531.77GRAS family transcription factor [more]
AT3G54220.12.8e-3030.50GRAS family transcription factor [more]
AT5G41920.16.9e-2929.02GRAS family transcription factor [more]
AT3G03450.14.5e-2826.92RGA-like 2 [more]
AT5G17490.17.7e-2828.87RGA-like protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 95..120
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 99..119
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 102..116
NoneNo IPR availablePANTHERPTHR31636:SF55OS12G0162700 PROTEINcoord: 10..503
IPR005202Transcription factor GRASPFAMPF03514GRAScoord: 136..502
e-value: 1.4E-69
score: 234.9
IPR005202Transcription factor GRASPANTHERPTHR31636OSJNBA0084A10.13 PROTEIN-RELATEDcoord: 10..503
IPR005202Transcription factor GRASPROSITEPS50985GRAScoord: 126..503
score: 45.947567

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0009936.1Tan0009936.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus