Tan0009823 (gene) Snake gourd v1

BLAST of Tan0009823 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 4.6e-30
Identity = 134/546 (24.54%), Postives = 234/546 (42.86%), Query Frame = 0

Query: 8   NCVVEIDELFRRDDLQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHELKPELQKM 67
           N    +D      DL+   E   SIF +P ++   +P +Y+P R+ +GP+H LKPEL +M
Sbjct: 24  NVQKSLDAELEEHDLE---EVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEM 83

Query: 68  EMLKLESAKKAKEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRILIDGLFL 127
           E  KL  A+K + ++    F  +V++L+ +E +IRACY+       E L W + +D  FL
Sbjct: 84  ERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVDSSFL 143

Query: 128 LRLLEISPDDAKEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNIT-MTQNEIVKDLLK 187
           +  L+I                                +R +E  I  +  NEI++D++ 
Sbjct: 144 IEFLKI------------------------------YSFRKVETLINRVGHNEILRDIMM 203

Query: 188 LENQIPIQILKRVLPRNLVEN------LHLLFYKFCVSVSPLAVYSGDDKDNSSELLEVF 247
           +ENQIP+ +L++ L   L         L  +    C  +SPL +   DD+   ++    F
Sbjct: 204 IENQIPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQ----F 263

Query: 248 DQSQHLLHFLYDLLFYQRITVYLE--------------------------YGISLKSLHV 307
            +  H+L FLY ++  +     LE                          +     S   
Sbjct: 264 QECNHILDFLYQMIVPRIEEEELEEDDEENRADENGGNRAIRFMDEIKHQFKRVFASRPA 323

Query: 308 DLL-----NMIAKILGIGLI---------NQLVEAFNLIQSLIGMIGGIRHSPSFETDSS 367
           DL+      +I+ + G   +          Q  EA    Q  + ++  I   P  E    
Sbjct: 324 DLILRFPWRIISNLPGFMALKLSADYLFTRQENEATTTRQESVSIL-DIEKPPLVE---E 383

Query: 368 PKFPTASELKRFKVKFK--------------------LPSITMNTFSEVILRNLLAFEVE 427
              P+ S+L +  V+FK                    LP I ++  +E +LRNL+A+E  
Sbjct: 384 LTIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEAT 443

Query: 428 AKLNPPCLSHYVKLMSGLIVTANDVTILKQAGIIAGGRSGDEEDVAKLFKELGDVAVKVS 487
               P   + Y +L++G+I +  DV +L++ G++      D+E  A+++  +        
Sbjct: 444 NTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQE-AAEMWNGM-------- 503

BLAST of Tan0009823 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 6.1e-06
Identity = 99/464 (21.34%), Postives = 175/464 (37.72%), Query Frame = 0

Query: 22  LQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHELKPELQKMEMLK-------LES 81
           L+ A +    IF++P+S    NP+AY P  + +GP+H  +  LQ ++  K       L+ 
Sbjct: 38  LESAGKESCCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDE 97

Query: 82  AKKAKEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRILIDGLFLLRLLEIS 141
           AKK        E   +V  +  LE +IR  Y+       + L + +++DG F+L +  I 
Sbjct: 98  AKKKD-----VEENVLVKAVVDLEDKIRKSYSEELKTGHD-LMFMMVLDGCFILMVFLIM 157

Query: 142 PDDAKEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNITMTQNEIVKDLLKLENQIPIQ 201
             + +  E               P+F+   L  S++            DLL LENQ+P  
Sbjct: 158 SGNIELSE--------------DPIFSIPWLLSSIQ-----------SDLLLLENQVPFF 217

Query: 202 ILKRVLPRNLVENLHLLFYKFCVSVSPLAVYSGDDKDNSSELLEVFDQSQHLLHFLYDLL 261
           +L+                          +Y G     SS+L  +        HF  + +
Sbjct: 218 VLQ-------------------------TLYVGSKIGVSSDLNRI------AFHFFKNPI 277

Query: 262 FYQRITVYLEYGISLKSLHVDLLNMIAK--ILGIGLINQLVEAFNLIQSLIGMIGGIRHS 321
              +   Y E   + K+ H  LL++I +  +      ++       +Q   G  G +   
Sbjct: 278 --DKEGSYWEKHRNYKAKH--LLDLIRETFLPNTSESDKASSPHVQVQLHEGKSGNV--- 337

Query: 322 PSFETDSSPKFPTASELKRFKVKFKL--------------------PSITMNTFSEVILR 381
           PS ++ + P   +A  L+   +KF+L                    P +  + F      
Sbjct: 338 PSVDSKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQLRFDGFISSFFL 397

Query: 382 NLLAFEVEAKLNPPCLSHYVKLMSGLIVTANDVTILKQAGIIAGGRSGDEEDVAKLFKEL 441
           N +AFE     +   ++ Y+  M  L+    DVT L+   +I     G   +V++ FK +
Sbjct: 398 NCVAFEQFYTDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSEFFKTI 420

Query: 442 GDVAVKVSSDDNESSSNFDTRMNFELNIMEKINEY----YENRW 453
               V            F+   ++  N+ + +NEY    Y   W
Sbjct: 458 SKDVV------------FEVDTSYLNNVFKGVNEYTKKWYNGLW 420

BLAST of Tan0009823 vs. NCBI nr
Match: KAG6592376.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 587.0 bits (1512), Expect = 1.5e-163
Identity = 323/524 (61.64%), Postives = 392/524 (74.81%), Query Frame = 0

Query: 1   MAEISRTNCVVEIDELFRRDDLQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHEL 60
           MA  S  N VVE+ EL RRD L++ALETPTSIF LPDSVTK+NPQAY+PSRL LGPFHEL
Sbjct: 1   MAGTSGMNWVVEVGELLRRDGLRMALETPTSIFMLPDSVTKENPQAYTPSRLLLGPFHEL 60

Query: 61  KPELQKMEMLKLESAKKAKEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRI 120
             EL KME+ KLE AK  KE+FQLP+ T MVD+LK+LE  IRA Y  N  + S+ALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPDLTLMVDQLKELEVPIRASYGANLAIGSDALASRI 120

Query: 121 LIDGLFLLRLLEISPDDAKEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNI-TMTQNE 180
           LIDGLFL+RLL+I  D+ K  +    +  NR + ++ PVFNE++LY  LE N+ TMTQNE
Sbjct: 121 LIDGLFLIRLLQIFADE-KNDQGSSDRLHNRRHKLELPVFNEKELYLKLERNMATMTQNE 180

Query: 181 IVKDLLKLENQIPIQILKRVLPRNLVENLHLLFYKFCVSVSPLAVYSGDDKDNSSE---- 240
           I+KDLLKLENQIPIQ+LKRVLP++L+ENL +LFYKFCV VSPL++ S D    S +    
Sbjct: 181 ILKDLLKLENQIPIQVLKRVLPKDLIENLPILFYKFCVFVSPLSLPSADKFTTSDDNPFL 240

Query: 241 -LLEVFDQSQHLLHFLYDLLFYQRITVYL-EYGISLKSLHVDLLNMIAKILGIGLINQLV 300
            L E+ DQSQHLLHF Y LL+YQR   Y+        SL V+LLN++AK++ +G++ QLV
Sbjct: 241 NLSEILDQSQHLLHFFYALLYYQRFLAYMPSQDYRSGSLFVELLNLLAKVVQVGVVQQLV 300

Query: 301 EAFNLIQSLIGMIGGIRHSPSFETDSSPKFPTASELKRFKVKFK---------------L 360
           EA  LIQSLIGM+G I HS  FET++SP FP+A++L++  VKF+               L
Sbjct: 301 EAVGLIQSLIGMLGKIHHSSPFETETSPGFPSATKLRKVGVKFRGGDGGGFDDESATLTL 360

Query: 361 PSITMNTFSEVILRNLLAFEVEAKLNPPCLSHYVKLMSGLIVTANDVTILKQAGIIAGGR 420
           P +T+N F EVILRNL+AFEVEAKLNP  LSHYVKLM+GLI T  DV ILK+AGII GGR
Sbjct: 361 PIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVKLMNGLIATTKDVGILKRAGIIVGGR 420

Query: 421 SGDEEDVAKLFKELGDVAVKVSSDDNESSSNFDTRMNFELNIMEKINEYYENRWRVKAER 480
           SGDE+DV KLFK+LG+V +K   D  E  SNF++ MNFE  I E+I +YYE+RW VKAER
Sbjct: 421 SGDEDDVVKLFKDLGNVVMKGGFDSEEGRSNFESYMNFERRIGEEIMKYYESRWMVKAER 480

Query: 481 FGKRYICPVFQALLILVVVVLILIVAVRTVCGWFSCPRIMGHSI 503
           F KRYI PV Q L+ILVVVVLILIVA+RTVCGWFSCP+IMGH I
Sbjct: 481 FVKRYIFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRI 523

BLAST of Tan0009823 vs. NCBI nr
Match: XP_022925375.1 (putative UPF0481 protein At3g02645 [Cucurbita moschata])

HSP 1 Score: 585.9 bits (1509), Expect = 3.4e-163
Identity = 324/524 (61.83%), Postives = 391/524 (74.62%), Query Frame = 0

Query: 1   MAEISRTNCVVEIDELFRRDDLQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHEL 60
           MA  S TN VVE+ EL RRD L++ALETPTSIFKLPDSVTK+NPQAY+PSRL LGPFHEL
Sbjct: 1   MAGTSGTNWVVEVGELLRRDGLRMALETPTSIFKLPDSVTKENPQAYTPSRLLLGPFHEL 60

Query: 61  KPELQKMEMLKLESAKKAKEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRI 120
             EL KME+ KLE AK  KE+FQLPE T MVD+LK+LE  IRA Y     + S+ALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGTKLAIGSDALASRI 120

Query: 121 LIDGLFLLRLLEISPDDAKEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNI-TMTQNE 180
           LIDGLFL+RLL+I  D+ K  +    +  NR + ++ PVF+EQ+LY  LEGN+ TMT+NE
Sbjct: 121 LIDGLFLIRLLQIFADE-KNDQGSSDRLHNRRHKLELPVFDEQELYLKLEGNMATMTRNE 180

Query: 181 IVKDLLKLENQIPIQILKRVLPRNLVENLHLLFYKFCVSVSPLAVYSGDDKDNSSE---- 240
           I+KDLLKLENQIPIQ+LKRVLP++L+ENL +LFYKFCV VSPL++ S D    S++    
Sbjct: 181 ILKDLLKLENQIPIQVLKRVLPKDLIENLPILFYKFCVFVSPLSLPSADKFTTSNDNPFL 240

Query: 241 -LLEVFDQSQHLLHFLYDLLFYQRITVYL-EYGISLKSLHVDLLNMIAKILGIGLINQLV 300
            L E+ DQSQHLLHFLY LL+YQR   Y         SL V+LLN++AK++ +G++ QLV
Sbjct: 241 NLSEILDQSQHLLHFLYALLYYQRFLAYRPSQDYRSGSLFVELLNLLAKVVQVGIVQQLV 300

Query: 301 EAFNLIQSLIGMIGGIRHSPSFETDSSPKFPTASELKRFKVKF---------------KL 360
           EA  LIQSLIGM+G I HS  FET++SP  P+A +L++  VKF                L
Sbjct: 301 EAVGLIQSLIGMLGKIHHSSPFETETSPGLPSAMKLRKVGVKFHGGDGGGFDDESATLTL 360

Query: 361 PSITMNTFSEVILRNLLAFEVEAKLNPPCLSHYVKLMSGLIVTANDVTILKQAGIIAGGR 420
           P +T+N F EVILRNL+AFEVEAKLNP  LSHYV LM+GLI T  DV ILK+AGII GGR
Sbjct: 361 PIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVTLMNGLIATTKDVGILKRAGIIVGGR 420

Query: 421 SGDEEDVAKLFKELGDVAVKVSSDDNESSSNFDTRMNFELNIMEKINEYYENRWRVKAER 480
           SGDE+DV KLFK+LG+V +K   D  E  SNF++ MNFE  I E+I +YYE+RW VKAER
Sbjct: 421 SGDEDDVVKLFKDLGNVVMKGGFDSEEGRSNFESYMNFERRISEEIMKYYESRWMVKAER 480

Query: 481 FGKRYICPVFQALLILVVVVLILIVAVRTVCGWFSCPRIMGHSI 503
           F KRYI PV Q L+ILVVVVLILIVA+RTVCGWFSCP+IMGH I
Sbjct: 481 FVKRYIFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRI 523

BLAST of Tan0009823 vs. NCBI nr
Match: XP_022973973.1 (UPF0481 protein At3g47200-like [Cucurbita maxima])

HSP 1 Score: 585.5 bits (1508), Expect = 4.4e-163
Identity = 324/524 (61.83%), Postives = 391/524 (74.62%), Query Frame = 0

Query: 1   MAEISRTNCVVEIDELFRRDDLQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHEL 60
           MAE S TN VVE+ EL RRD L++ALETPTSIFKLPDSVT  NPQAY+PSRL LGPFHEL
Sbjct: 1   MAETSETNWVVEVGELLRRDGLRMALETPTSIFKLPDSVT--NPQAYTPSRLLLGPFHEL 60

Query: 61  KPELQKMEMLKLESAKKAKEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRI 120
             EL KME+ KLE AK  KE+FQLPE T MVD+LK+LE  IRA Y  N  ++S+ALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGANLAIDSDALASRI 120

Query: 121 LIDGLFLLRLLEISPDDAKEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNI-TMTQNE 180
           LIDGLFL++LL+IS D+ K  E       NR + ++ PVFNEQ+LY  LEGN+ T+T+NE
Sbjct: 121 LIDGLFLIQLLQISADE-KNDEGSSDWLHNRRHKLELPVFNEQELYLKLEGNMATLTRNE 180

Query: 181 IVKDLLKLENQIPIQILKRVLPRNLVENLHLLFYKFCVSVSPLAVYSGDDKDNSSE---- 240
           I+KDLLKLENQIPIQ+LKRVLP++  ENL +LFYKFCV VSPL++ S D    S++    
Sbjct: 181 ILKDLLKLENQIPIQVLKRVLPKDFTENLPILFYKFCVFVSPLSLPSADKFTTSNDNVFL 240

Query: 241 -LLEVFDQSQHLLHFLYDLLFYQRITVYL-EYGISLKSLHVDLLNMIAKILGIGLINQLV 300
            L E+ DQSQHLLHF Y LL+YQR   Y+        SL V+LLN++AK++ +G++ QLV
Sbjct: 241 NLSEILDQSQHLLHFFYALLYYQRFLAYMPSQDYRSGSLFVELLNLLAKVVQVGVVQQLV 300

Query: 301 EAFNLIQSLIGMIGGIRHSPSFETDSSPKFPTASELKRFKVKFK---------------L 360
           EA  LIQSLIGM+G I HS  FET++SP FP+A++L++  VKF+               L
Sbjct: 301 EAVGLIQSLIGMLGKIHHSSPFETETSPGFPSATKLRKVGVKFRGGDGGGFNDESATLTL 360

Query: 361 PSITMNTFSEVILRNLLAFEVEAKLNPPCLSHYVKLMSGLIVTANDVTILKQAGIIAGGR 420
           P +T+N F EVILRNL+AFEVEAKLNP  LSHYVKLM+GLI T  DV ILK+AGII  GR
Sbjct: 361 PIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVKLMNGLIATTKDVGILKRAGIIVSGR 420

Query: 421 SGDEEDVAKLFKELGDVAVKVSSDDNESSSNFDTRMNFELNIMEKINEYYENRWRVKAER 480
           SGDE+DV KLFK+LG+V VK   D  E  SNF++ MNFE  I E+I +YYE RW VK ER
Sbjct: 421 SGDEDDVVKLFKDLGNVVVKGGFDSEEGRSNFESFMNFERRIGEEIVKYYEGRWMVKGER 480

Query: 481 FGKRYICPVFQALLILVVVVLILIVAVRTVCGWFSCPRIMGHSI 503
           F KRY+ PV Q L+ILVVVVLILIVA+RTVCGWFSCP+IMGH I
Sbjct: 481 FVKRYVFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRI 521

BLAST of Tan0009823 vs. NCBI nr
Match: XP_023535329.1 (putative UPF0481 protein At3g02645 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 546.6 bits (1407), Expect = 2.3e-151
Identity = 316/556 (56.83%), Postives = 382/556 (68.71%), Query Frame = 0

Query: 1   MAEISRTNCVVEIDELFRRDDLQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHEL 60
           MA  S TN VVE+ EL RRD L++ALETPTSIFKLPDSVTK+NPQAYSPSRL LGPFHEL
Sbjct: 1   MAGTSETNWVVEVGELLRRDGLRMALETPTSIFKLPDSVTKENPQAYSPSRLLLGPFHEL 60

Query: 61  KPELQKMEMLKLESAKKAKEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRI 120
             EL KME+ KLE AK  KE+FQLPE T MVD+LK+LE  IRA Y  N  + S+ALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGANLAIGSDALASRI 120

Query: 121 LIDGLFLLRLLEISPD------------------------------------------DA 180
           LIDGLFL+ LL+I  D                                          + 
Sbjct: 121 LIDGLFLIWLLQIYADEKNYPGILQGLSTEDELGPKIRPKGVVDGQQVNIPVLPLVGPEG 180

Query: 181 KEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNI-TMTQNEIVKDLLKLENQIPIQILK 240
           + +     +  NR Y ++ PVF+EQ+LY  LEGN+ TMT+NEI+KDLLKLENQIPIQ+LK
Sbjct: 181 RRRLGSSDRLHNRRYKLELPVFDEQELYLKLEGNMATMTRNEILKDLLKLENQIPIQVLK 240

Query: 241 RVLPRNLVENLHLLFYKFCVSVSPLAVYSGDDKDNSSE-----LLEVFDQSQHLLHFLYD 300
           RVLP++L+ENL +LFYKFCV VSPL++ S D    S++     L E+ DQSQHLLHFLY 
Sbjct: 241 RVLPKDLIENLPILFYKFCVFVSPLSLPSADKFTTSNDNPFLNLSEILDQSQHLLHFLYA 300

Query: 301 LLFYQRITVYL-EYGISLKSLHVDLLNMIAKILGIGLINQLVEAFNLIQSLIGMIGGIRH 360
           LL+YQR   Y         SL V+LLN++AK++ +G++ QLVEA  LIQSLIGM+G I H
Sbjct: 301 LLYYQRFLAYRPSQDYRSGSLFVELLNLLAKVVQVGIVQQLVEAVGLIQSLIGMLGKIHH 360

Query: 361 SPSFETDSSPKFPTASELKRFKVKF---------------KLPSITMNTFSEVILRNLLA 420
           S  FET++SP  P+A++L++  VKF                LPS+T+N F EVILRNL+A
Sbjct: 361 SSPFETETSPGLPSATKLRKVGVKFHGGDGGGFDDESATLTLPSVTINDFYEVILRNLVA 420

Query: 421 FEVEAKLNPPCLSHYVKLMSGLIVTANDVTILKQAGIIAGGRSGDEEDVAKLFKELGDVA 480
           FEVEAK+NP  LSHYV LM+GLI T  DV ILK+AGII GGRSGDE+DV KLFK+LG+V 
Sbjct: 421 FEVEAKMNPSSLSHYVTLMNGLIATTKDVGILKRAGIIVGGRSGDEDDVVKLFKDLGNVV 480

Query: 481 VKVSSDDNESSSNFDTRMNFELNIMEKINEYYENRWRVKAERFGKRYICPVFQALLILVV 493
           VK   D  E  SNF++ MNFE  I E+I +YYE+RW VKAERF KRYI PV Q L+ILVV
Sbjct: 481 VKGGFDSEEGRSNFESFMNFEGRIGEEIMKYYESRWMVKAERFVKRYIFPVLQGLMILVV 540

BLAST of Tan0009823 vs. NCBI nr
Match: KAG7025194.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 506.9 bits (1304), Expect = 2.0e-139
Identity = 291/516 (56.40%), Postives = 352/516 (68.22%), Query Frame = 0

Query: 1   MAEISRTNCVVEIDELFRRDDLQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHEL 60
           MA  S TN VVE+ EL RRD L++ALETPTSIF LPDSVTK+NPQAY+PSRL LGPFHEL
Sbjct: 1   MAGTSGTNWVVEVGELLRRDGLRMALETPTSIFMLPDSVTKENPQAYTPSRLLLGPFHEL 60

Query: 61  KPELQKMEMLKLESAKKAKEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRI 120
             EL KME+ KLE AK  KE+FQLP+ T MVD+LK+LE  IRA Y     + S+ALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPDLTLMVDQLKELEVPIRASYGTKLAIGSDALASRI 120

Query: 121 LIDGLFLLRLLEISPDDAKEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNITMTQNEI 180
           LIDGLFL+RLL+I  D+  +                                        
Sbjct: 121 LIDGLFLIRLLQIFADEKND---------------------------------------- 180

Query: 181 VKDLLKLENQIPIQILKRVLPRNLVENLHLLFYKFCVSVSPLAVYSGDDKDNSSE----- 240
            +DLLKLENQIPIQ+LKRVLP++L+ENL +LFYKFCV VSPL++ S D    S +     
Sbjct: 181 -QDLLKLENQIPIQVLKRVLPKDLIENLPILFYKFCVFVSPLSLPSADKFTTSDDNPFLN 240

Query: 241 LLEVFDQSQHLLHFLYDLLFYQRITVYL-EYGISLKSLHVDLLNMIAKILGIGLINQLVE 300
           L E+ DQSQHLLHF Y LL+YQR   Y+        SL V+LLN++AK++ +G++ QLVE
Sbjct: 241 LSEILDQSQHLLHFFYALLYYQRFLAYMPSQDYRSGSLFVELLNLLAKVVQVGVVQQLVE 300

Query: 301 AFNLIQSLIGMIGGIRHSPSFETDSSPKFPTASELKRFKVKFK---------------LP 360
           A  LIQSLIGM+G I HS  FET++SP FP+A++L++  VKF+               LP
Sbjct: 301 AVGLIQSLIGMLGKIHHSSPFETETSPGFPSATKLRKVGVKFRGGDGGGFDDESATLTLP 360

Query: 361 SITMNTFSEVILRNLLAFEVEAKLNPPCLSHYVKLMSGLIVTANDVTILKQAGIIAGGRS 420
            +T+N F EVILRNL+AFEVEAKLNP  LSHYV LM+GLI T  DV ILK+AGII GGRS
Sbjct: 361 IVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVTLMNGLIATTKDVGILKRAGIIVGGRS 420

Query: 421 GDEEDVAKLFKELGDVAVKVSSDDNESSSNFDTRMNFELNIMEKINEYYENRWRVKAERF 480
           GDE+DV KLFK+LG+V +K   D  E  SNF++ MNFE  I E+I +YYE+RW VKAERF
Sbjct: 421 GDEDDVVKLFKDLGNVVMKGGFDSEEGRSNFESYMNFERRIGEEIMKYYESRWMVKAERF 475

Query: 481 GKRYICPVFQALLILVVVVLILIVAVRTVCGWFSCP 496
            KRYI PV Q L+ILVVVVLILIVA+RTVC     P
Sbjct: 481 VKRYIFPVLQGLMILVVVVLILIVAIRTVCELHKTP 475

BLAST of Tan0009823 vs. ExPASy TrEMBL
Match: A0A6J1EHS6 (putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111432682 PE=4 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.6e-163
Identity = 324/524 (61.83%), Postives = 391/524 (74.62%), Query Frame = 0

Query: 1   MAEISRTNCVVEIDELFRRDDLQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHEL 60
           MA  S TN VVE+ EL RRD L++ALETPTSIFKLPDSVTK+NPQAY+PSRL LGPFHEL
Sbjct: 1   MAGTSGTNWVVEVGELLRRDGLRMALETPTSIFKLPDSVTKENPQAYTPSRLLLGPFHEL 60

Query: 61  KPELQKMEMLKLESAKKAKEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRI 120
             EL KME+ KLE AK  KE+FQLPE T MVD+LK+LE  IRA Y     + S+ALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGTKLAIGSDALASRI 120

Query: 121 LIDGLFLLRLLEISPDDAKEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNI-TMTQNE 180
           LIDGLFL+RLL+I  D+ K  +    +  NR + ++ PVF+EQ+LY  LEGN+ TMT+NE
Sbjct: 121 LIDGLFLIRLLQIFADE-KNDQGSSDRLHNRRHKLELPVFDEQELYLKLEGNMATMTRNE 180

Query: 181 IVKDLLKLENQIPIQILKRVLPRNLVENLHLLFYKFCVSVSPLAVYSGDDKDNSSE---- 240
           I+KDLLKLENQIPIQ+LKRVLP++L+ENL +LFYKFCV VSPL++ S D    S++    
Sbjct: 181 ILKDLLKLENQIPIQVLKRVLPKDLIENLPILFYKFCVFVSPLSLPSADKFTTSNDNPFL 240

Query: 241 -LLEVFDQSQHLLHFLYDLLFYQRITVYL-EYGISLKSLHVDLLNMIAKILGIGLINQLV 300
            L E+ DQSQHLLHFLY LL+YQR   Y         SL V+LLN++AK++ +G++ QLV
Sbjct: 241 NLSEILDQSQHLLHFLYALLYYQRFLAYRPSQDYRSGSLFVELLNLLAKVVQVGIVQQLV 300

Query: 301 EAFNLIQSLIGMIGGIRHSPSFETDSSPKFPTASELKRFKVKF---------------KL 360
           EA  LIQSLIGM+G I HS  FET++SP  P+A +L++  VKF                L
Sbjct: 301 EAVGLIQSLIGMLGKIHHSSPFETETSPGLPSAMKLRKVGVKFHGGDGGGFDDESATLTL 360

Query: 361 PSITMNTFSEVILRNLLAFEVEAKLNPPCLSHYVKLMSGLIVTANDVTILKQAGIIAGGR 420
           P +T+N F EVILRNL+AFEVEAKLNP  LSHYV LM+GLI T  DV ILK+AGII GGR
Sbjct: 361 PIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVTLMNGLIATTKDVGILKRAGIIVGGR 420

Query: 421 SGDEEDVAKLFKELGDVAVKVSSDDNESSSNFDTRMNFELNIMEKINEYYENRWRVKAER 480
           SGDE+DV KLFK+LG+V +K   D  E  SNF++ MNFE  I E+I +YYE+RW VKAER
Sbjct: 421 SGDEDDVVKLFKDLGNVVMKGGFDSEEGRSNFESYMNFERRISEEIMKYYESRWMVKAER 480

Query: 481 FGKRYICPVFQALLILVVVVLILIVAVRTVCGWFSCPRIMGHSI 503
           F KRYI PV Q L+ILVVVVLILIVA+RTVCGWFSCP+IMGH I
Sbjct: 481 FVKRYIFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRI 523

BLAST of Tan0009823 vs. ExPASy TrEMBL
Match: A0A6J1IEU8 (UPF0481 protein At3g47200-like OS=Cucurbita maxima OX=3661 GN=LOC111472588 PE=4 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 2.1e-163
Identity = 324/524 (61.83%), Postives = 391/524 (74.62%), Query Frame = 0

Query: 1   MAEISRTNCVVEIDELFRRDDLQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHEL 60
           MAE S TN VVE+ EL RRD L++ALETPTSIFKLPDSVT  NPQAY+PSRL LGPFHEL
Sbjct: 1   MAETSETNWVVEVGELLRRDGLRMALETPTSIFKLPDSVT--NPQAYTPSRLLLGPFHEL 60

Query: 61  KPELQKMEMLKLESAKKAKEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRI 120
             EL KME+ KLE AK  KE+FQLPE T MVD+LK+LE  IRA Y  N  ++S+ALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGANLAIDSDALASRI 120

Query: 121 LIDGLFLLRLLEISPDDAKEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNI-TMTQNE 180
           LIDGLFL++LL+IS D+ K  E       NR + ++ PVFNEQ+LY  LEGN+ T+T+NE
Sbjct: 121 LIDGLFLIQLLQISADE-KNDEGSSDWLHNRRHKLELPVFNEQELYLKLEGNMATLTRNE 180

Query: 181 IVKDLLKLENQIPIQILKRVLPRNLVENLHLLFYKFCVSVSPLAVYSGDDKDNSSE---- 240
           I+KDLLKLENQIPIQ+LKRVLP++  ENL +LFYKFCV VSPL++ S D    S++    
Sbjct: 181 ILKDLLKLENQIPIQVLKRVLPKDFTENLPILFYKFCVFVSPLSLPSADKFTTSNDNVFL 240

Query: 241 -LLEVFDQSQHLLHFLYDLLFYQRITVYL-EYGISLKSLHVDLLNMIAKILGIGLINQLV 300
            L E+ DQSQHLLHF Y LL+YQR   Y+        SL V+LLN++AK++ +G++ QLV
Sbjct: 241 NLSEILDQSQHLLHFFYALLYYQRFLAYMPSQDYRSGSLFVELLNLLAKVVQVGVVQQLV 300

Query: 301 EAFNLIQSLIGMIGGIRHSPSFETDSSPKFPTASELKRFKVKFK---------------L 360
           EA  LIQSLIGM+G I HS  FET++SP FP+A++L++  VKF+               L
Sbjct: 301 EAVGLIQSLIGMLGKIHHSSPFETETSPGFPSATKLRKVGVKFRGGDGGGFNDESATLTL 360

Query: 361 PSITMNTFSEVILRNLLAFEVEAKLNPPCLSHYVKLMSGLIVTANDVTILKQAGIIAGGR 420
           P +T+N F EVILRNL+AFEVEAKLNP  LSHYVKLM+GLI T  DV ILK+AGII  GR
Sbjct: 361 PIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVKLMNGLIATTKDVGILKRAGIIVSGR 420

Query: 421 SGDEEDVAKLFKELGDVAVKVSSDDNESSSNFDTRMNFELNIMEKINEYYENRWRVKAER 480
           SGDE+DV KLFK+LG+V VK   D  E  SNF++ MNFE  I E+I +YYE RW VK ER
Sbjct: 421 SGDEDDVVKLFKDLGNVVVKGGFDSEEGRSNFESFMNFERRIGEEIVKYYEGRWMVKGER 480

Query: 481 FGKRYICPVFQALLILVVVVLILIVAVRTVCGWFSCPRIMGHSI 503
           F KRY+ PV Q L+ILVVVVLILIVA+RTVCGWFSCP+IMGH I
Sbjct: 481 FVKRYVFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRI 521

BLAST of Tan0009823 vs. ExPASy TrEMBL
Match: A0A6J1DUS9 (putative UPF0481 protein At3g02645 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023673 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 3.2e-103
Identity = 238/528 (45.08%), Postives = 338/528 (64.02%), Query Frame = 0

Query: 4   ISRTNCVVEIDELFRRDDLQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHELKPE 63
           +  T  +V+I +L   ++LQ AL+TPTSIFK+ DS+TK+NP+AY P R+ LGP+H  K +
Sbjct: 29  LHETLWLVQISQLLEPNNLQTALQTPTSIFKIADSITKNNPEAYFPRRVALGPYHHFKND 88

Query: 64  LQKMEMLKLESAKKAKEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRILID 123
           L KM++ KL +  KA+E F L E + ++DELK LE +IR+C++   D+ SEALA  +LID
Sbjct: 89  LHKMQLFKLHNTIKARETFHLQELSHIMDELKHLEIKIRSCFDHFLDIGSEALARIMLID 148

Query: 124 GLFLLRLLEISPDDAKEKEKKGKKNSNRPYGIDSPVFNEQQLYRSL-EGNITMTQNEIVK 183
           GLFL++LL IS D+ K++ +  + +   P     P+F EQQLY  L EGN+ MTQ+E VK
Sbjct: 149 GLFLIQLLVISTDE-KDELRSARPDLCLPSPF--PLFVEQQLYSHLIEGNL-MTQDEFVK 208

Query: 184 DLLKLENQIPIQILKRVLPRNLVENLHLLFYKFCVSVSPLAVYSGDDKDNSS-----ELL 243
           D+LKLENQIP+ +LK +LP N   NLHLLF+KFC  VSP  +   D  D  S      L 
Sbjct: 209 DILKLENQIPLFVLKTILPENFGNNLHLLFFKFCNFVSPKQLPPHDMPDLMSLSGYTNLS 268

Query: 244 EVFDQSQHLLHFLYDLLFYQRITVYLEYGISL--KSLHVDLLNMIAKILGIGLINQLVEA 303
           ++ DQS HLLHFLY L+  +R++V     ++L   S  V+LLN+++ +L IG + QL EA
Sbjct: 269 QIIDQSHHLLHFLYLLILDRRLSVDQAVAMALFRTSFLVELLNVLSSVLQIGFLQQLSEA 328

Query: 304 FNLIQSLIGMIGGIRHSPSF---ETDSSPKFPTASELKRFKVKF---------------- 363
             L+QSL G++G +  S S    + DS P  P+ASELKR +VKF                
Sbjct: 329 AGLLQSLFGLLGRVGSSSSSSGEDMDSPPLIPSASELKRVRVKFDGRYNDFHESMRFEQE 388

Query: 364 ------KLPSITMNTFSEVILRNLLAFEVEAKLNPPCLSHYVKLMSGLIVTANDVTILKQ 423
                 ++PS+ +N FSEV+ RNL+AFE   K N    + YV LMSGLI TA DV ILKQ
Sbjct: 389 SKKVCLEMPSMAINVFSEVVFRNLVAFEAATKKNATWFAFYVALMSGLITTAKDVKILKQ 448

Query: 424 AGIIAGGRSGDEEDVAKLFKELGDVAVKVSSDDNESSSNFDTRMNFELNIMEKINEYYEN 483
           A II     G EE+V KLF  L ++ +++ +     SS     M  E+ I++ IN YYE+
Sbjct: 449 AEIIE-SHWGSEEEVVKLFDGLRNL-LELHNQQMSYSSGRIMEM-AEIQIVKDINFYYES 508

Query: 484 RWRVKAERFGKRYICPVFQALLILVVVVLILIVAVRTVCGWFSCPRIM 499
            W+VKA+RF KR++ P+ + + +++V++LI++V +RT CGWFSC R++
Sbjct: 509 CWKVKAKRFIKRFVNPILKVIFLILVILLIVVVVLRTSCGWFSCSRVL 549

BLAST of Tan0009823 vs. ExPASy TrEMBL
Match: A0A6J1IEU4 (putative UPF0481 protein At3g02645 OS=Cucurbita maxima OX=3661 GN=LOC111472585 PE=4 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 3.4e-100
Identity = 227/525 (43.24%), Postives = 324/525 (61.71%), Query Frame = 0

Query: 6   RTNCVVEIDELFRRDDLQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHELKPELQ 65
           +TN VV+I++L   ++LQ AL+TPTSIFKLP S+T   PQAY P  + LGP+H  K EL 
Sbjct: 14  KTNWVVQINQLLDPNNLQTALQTPTSIFKLPHSITNGMPQAYFPCHIALGPYHHFKNELY 73

Query: 66  KMEMLKLESAKKAKEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRILIDGL 125
           +ME+ KL++A  A E F+LP+  Q+  +L++ + +IR+CY+   D  +E LAW +LIDGL
Sbjct: 74  QMELYKLDNAINAIESFKLPQLHQIAADLEQFDIKIRSCYDKFLDFGAEVLAWIMLIDGL 133

Query: 126 FLLRLLEISPDDAKEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNITMTQNEIVKDLL 185
           FL++LL IS     EK++      +       P+F EQ+LY SL     MTQ+E+V D+L
Sbjct: 134 FLVQLLAIS---NHEKDELRTMRQDLYLPSPFPLFVEQKLYGSLIEANLMTQDELVNDIL 193

Query: 186 KLENQIPIQILKRVLPRNLVENLHLLFYKFCVSVSPLAVYSGDDK-----DNSSELLEVF 245
           KLENQIPI +LK++LP+NL  NLH+LF++FC  VSP  +   D          ++L ++ 
Sbjct: 194 KLENQIPIFVLKKILPQNLSNNLHILFFRFCEVVSPKKLPPHDSPQFMRYSGYTDLYQIL 253

Query: 246 DQSQHLLHFLYDLLFYQRITVYLEYGISL--KSLHVDLLNMIAKILGIGLINQLVEAFNL 305
           +QS HLLHFLY L+    +++    G++L   S  V+LLN +A +L I L+ QL EA  L
Sbjct: 254 EQSHHLLHFLYLLILDSSLSIDRPVGMALLRGSFLVELLNALASVLQIALLQQLSEAVGL 313

Query: 306 IQSLIGMIGGIRHSPSF---ETDSSPKFPTASELKRFKVK-------------------- 365
           IQSL+G++G +  S S    + +  P  P+A +L+R  VK                    
Sbjct: 314 IQSLLGLLGRVGSSSSSTGEDMNGPPLIPSAEQLRRVGVKLEGNYEDFHESISFEKERRE 373

Query: 366 --FKLPSITMNTFSEVILRNLLAFEVEAKLNPPCLSHYVKLMSGLIVTANDVTILKQAGI 425
              KLP+I +N FSEV+ RNL+AFE   KLNPPC S+YV L+SGLI T  DV ILK+A I
Sbjct: 374 PCLKLPTIVINAFSEVVYRNLVAFEGATKLNPPCFSNYVALISGLITTTKDVKILKKASI 433

Query: 426 IAGGRSGDEEDVAKLFKELGDVAVKVSSDDNESSSNFDTRMNFELNIMEKINEYYENRWR 485
           I    +G EE+V KLF  L  V               + +   EL +++ IN YYE+ W+
Sbjct: 434 IE-SHAGSEEEVVKLFDGLRSV--------------LELKNMAELQMVKDINSYYESCWK 493

Query: 486 VKAERFGKRYICPVFQALLILVVVVLILIVAVRTVCGWFSCPRIM 499
           VKA+RF ++YI P+ + ++++VV++LI +V VRT CGWF C RI+
Sbjct: 494 VKAKRFIQKYINPMLKVIVLVVVILLIGVVVVRTFCGWFGCSRIL 520

BLAST of Tan0009823 vs. ExPASy TrEMBL
Match: A0A6J1EBZ5 (putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111432681 PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 1.2e-97
Identity = 222/524 (42.37%), Postives = 320/524 (61.07%), Query Frame = 0

Query: 7   TNCVVEIDELFRRDDLQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHELKPELQK 66
           TN VV+I++L   ++LQ AL+TPTSIFKLP+S+T   PQAY P  + LGP+H  + EL +
Sbjct: 15  TNWVVQINQLLDPNNLQTALQTPTSIFKLPNSITNSMPQAYFPRHIALGPYHHFQNELYQ 74

Query: 67  MEMLKLESAKKAKEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRILIDGLF 126
           ME+ KL++A  A E F+LP+  Q+  +L++ + +IR+CYN   D  +EALAW +LIDGLF
Sbjct: 75  MELYKLDNAINAIESFKLPQLHQIAADLEQFDIKIRSCYNKFLDFGAEALAWIMLIDGLF 134

Query: 127 LLRLLEISPDDAKEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNITMTQNEIVKDLLK 186
           L+++L IS D   E ++  +   +       P+F EQ+LY SL     MTQ+E+V D+LK
Sbjct: 135 LVQILAISND---ENDELRRMRQDLYLPSPFPLFVEQKLYCSLIEANLMTQDELVNDILK 194

Query: 187 LENQIPIQILKRVLPRNLVENLHLLFYKFCVSVSPLAVYSGDDK-----DNSSELLEVFD 246
           LENQIP  +LK++LP+NL  NLH+LF++FC  VSP  +   D          ++L ++ +
Sbjct: 195 LENQIPSFVLKKILPQNLSNNLHILFFRFCEVVSPKKLPPHDSPQFMRYSGYTDLYQILE 254

Query: 247 QSQHLLHFLYDLLFYQRITVYLEYGISL--KSLHVDLLNMIAKILGIGLINQLVEAFNLI 306
           QS HLLHFLY L+    +++    G++L   S  V+LLN +A +L I  + QL EA  LI
Sbjct: 255 QSHHLLHFLYLLILDSSLSIDRPVGMALLRGSFLVELLNALASVLQIAFLQQLSEAVGLI 314

Query: 307 QSLIGM---IGGIRHSPSFETDSSPKFPTASELKRFKVK--------------------- 366
           QSL+G+   +G    S   + +  P  P+A +L+R  VK                     
Sbjct: 315 QSLLGLFRRVGSSSSSIGEDMNGPPLIPSAEQLRRVGVKLEGNYEDFHESISFEKERRET 374

Query: 367 -FKLPSITMNTFSEVILRNLLAFEVEAKLNPPCLSHYVKLMSGLIVTANDVTILKQAGII 426
             K P+I +N FSEV+  NL+AFE   KLNPPC S+YV LMSGLI T  DV ILK+A II
Sbjct: 375 CLKFPTIVINAFSEVVYSNLVAFEGATKLNPPCFSNYVALMSGLITTIKDVKILKEASII 434

Query: 427 AGGRSGDEEDVAKLFKELGDVAVKVSSDDNESSSNFDTRMNFELNIMEKINEYYENRWRV 486
                G+EE+V KLF  L  V               + +   E+ +++ IN YYE+ W+V
Sbjct: 435 E-SHIGNEEEVVKLFDGLRSV--------------LELKNMAEIQMVKDINSYYESCWKV 494

Query: 487 KAERFGKRYICPVFQALLILVVVVLILIVAVRTVCGWFSCPRIM 499
           KA+RF ++YI P+ + ++++VV++LI +V VRT CGWF C RI+
Sbjct: 495 KAKRFIQKYINPMLKVIVLVVVILLIGVVVVRTFCGWFGCLRIL 520

BLAST of Tan0009823 vs. TAIR 10
Match: AT3G02645.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 134.0 bits (336), Expect = 3.3e-31
Identity = 134/546 (24.54%), Postives = 234/546 (42.86%), Query Frame = 0

Query: 8   NCVVEIDELFRRDDLQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHELKPELQKM 67
           N    +D      DL+   E   SIF +P ++   +P +Y+P R+ +GP+H LKPEL +M
Sbjct: 24  NVQKSLDAELEEHDLE---EVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEM 83

Query: 68  EMLKLESAKKAKEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRILIDGLFL 127
           E  KL  A+K + ++    F  +V++L+ +E +IRACY+       E L W + +D  FL
Sbjct: 84  ERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVDSSFL 143

Query: 128 LRLLEISPDDAKEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNIT-MTQNEIVKDLLK 187
           +  L+I                                +R +E  I  +  NEI++D++ 
Sbjct: 144 IEFLKI------------------------------YSFRKVETLINRVGHNEILRDIMM 203

Query: 188 LENQIPIQILKRVLPRNLVEN------LHLLFYKFCVSVSPLAVYSGDDKDNSSELLEVF 247
           +ENQIP+ +L++ L   L         L  +    C  +SPL +   DD+   ++    F
Sbjct: 204 IENQIPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQ----F 263

Query: 248 DQSQHLLHFLYDLLFYQRITVYLE--------------------------YGISLKSLHV 307
            +  H+L FLY ++  +     LE                          +     S   
Sbjct: 264 QECNHILDFLYQMIVPRIEEEELEEDDEENRADENGGNRAIRFMDEIKHQFKRVFASRPA 323

Query: 308 DLL-----NMIAKILGIGLI---------NQLVEAFNLIQSLIGMIGGIRHSPSFETDSS 367
           DL+      +I+ + G   +          Q  EA    Q  + ++  I   P  E    
Sbjct: 324 DLILRFPWRIISNLPGFMALKLSADYLFTRQENEATTTRQESVSIL-DIEKPPLVE---E 383

Query: 368 PKFPTASELKRFKVKFK--------------------LPSITMNTFSEVILRNLLAFEVE 427
              P+ S+L +  V+FK                    LP I ++  +E +LRNL+A+E  
Sbjct: 384 LTIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEAT 443

Query: 428 AKLNPPCLSHYVKLMSGLIVTANDVTILKQAGIIAGGRSGDEEDVAKLFKELGDVAVKVS 487
               P   + Y +L++G+I +  DV +L++ G++      D+E  A+++  +        
Sbjct: 444 NTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQE-AAEMWNGM-------- 503

BLAST of Tan0009823 vs. TAIR 10
Match: AT3G50130.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 78.2 bits (191), Expect = 2.1e-14
Identity = 110/500 (22.00%), Postives = 204/500 (40.80%), Query Frame = 0

Query: 19  RDDLQLAL--ETPTS-----IFKLPDSVTKDNPQAYSPSRLFLGPFHELKPELQKMEMLK 78
           RD ++ AL  +  TS     I+++P  + ++N ++Y P  + LGPFH     L  M+  K
Sbjct: 121 RDKMEQALREDATTSWDKLCIYRVPQYLQENNKKSYFPQTVSLGPFHHGNKHLLPMDRHK 180

Query: 79  LESAKKAKEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRILIDGLFLLRLL 138
             +      + +  +    +D +K+LE   RACY G  D+ S   +  +++DG F+L L 
Sbjct: 181 WRAVNMVMARTK-HDIEMYIDAMKELEDRARACYEGPIDLSSNKFSEMLVLDGCFVLELF 240

Query: 139 EISPDDAKEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNITMTQNEIVKDLLKLENQI 198
             + +   E            Y  + PVF       ++ G++    + I +D++ LENQ+
Sbjct: 241 RGADEGFSE----------LGYDRNDPVF-------AMRGSM----HSIQRDMVMLENQL 300

Query: 199 PIQILKRVL--------PRNLVENLHLLFYKFCVSVSPLAVYSGDDKDNSSELLEVFDQS 258
           P+ +L R+L           LV  L + F+   +        + D  +       + D+ 
Sbjct: 301 PLFVLNRLLEIQLGKRHQTGLVSRLAVRFFDPLMPTDEPLTKTDDSLEQDKFFNPIADKD 360

Query: 259 QHLLHFLYDLLFYQRITVYLEYGISLKSLHVDLLNMIAKILGIGLINQLVEAFNLIQSLI 318
           +  LH L                +  ++L     N   ++  +    +   A    Q LI
Sbjct: 361 KGELHCL---------------DVFRRNLLRPCSNPEPRLSRMRWSWRTRVADKRQQQLI 420

Query: 319 GMIGGIRHSPSFETDSSPKFPTASELKRFKVKFK-----LPSITMNTFSEVILRNLLAFE 378
             +  +R       ++  KF T    + + ++FK     +P + ++  ++ +  NL+AFE
Sbjct: 421 HCVTELR-------EAGIKFRTRKTDRFWDIRFKNGYLEIPKLLIHDGTKSLFSNLIAFE 480

Query: 379 VEAKLNPPCLSHYVKLMSGLIVTANDVTILKQAGIIAGGRSGDEEDVAKLFKELGDVAVK 438
                +   ++ Y+  M  LI ++ DV  L   GII     G++ +VA LF  L      
Sbjct: 481 QCHIDSSNDITSYIIFMDNLIDSSEDVRYLHYCGIIEHW-LGNDYEVADLFNRLCQEVA- 540

Query: 439 VSSDDNESSSNFDTRMNFELNIMEKINEYYENRWRVKAERFGKRYI---CPVFQALLILV 496
                      FD + ++   +  K++  Y  +W V       +Y       F     LV
Sbjct: 541 -----------FDPQNSYLSQLSNKVDRNYSRKWNVLKAILKHKYFNNPWAYFSFFAALV 563

BLAST of Tan0009823 vs. TAIR 10
Match: AT3G47250.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 70.5 bits (171), Expect = 4.4e-12
Identity = 112/469 (23.88%), Postives = 192/469 (40.94%), Query Frame = 0

Query: 22  LQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHELKPELQKMEMLK---LESAKKA 81
           L+ A +    IF++PDS+ + NP+AY P  + +GP+H  +  LQ ++  K   LE     
Sbjct: 53  LESAGKASCCIFRIPDSLAEVNPKAYKPKVVSIGPYHYGENHLQMIQQHKFRFLELFVDR 112

Query: 82  KEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRILIDGLFLLRLLEISPDDA 141
             K  + E   +   +  L+ +IRA Y+    +E   L   +++DG F+L LL I     
Sbjct: 113 ATKKGMDE-NVLYAAVGALQHKIRASYSEELRIEKSELVSMMILDGCFILMLLLI----- 172

Query: 142 KEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNITMTQNEIVKDLLKLENQIPIQILKR 201
               +K   + N+      P+F    +  S++            DLL LENQ+P  +L+ 
Sbjct: 173 --VSRKIDLDMNK-----DPIFTIPWILASIQ-----------SDLLLLENQVPFFVLRT 232

Query: 202 VLPRNLV----ENLHLLFYKFCVSVSPLAVYSGDDKDNSSELLEVFDQSQHLLHFLYDLL 261
           +  ++ +    +   + F  F +S+     Y    +D           ++HLL    DL 
Sbjct: 233 IFDKSGIGSPGDLNRMAFSFFNLSIDKPDTYWAKHRDRG---------AKHLL----DLN 292

Query: 262 FYQRITVYLEYGISLKSLHVDLLNMIAKILGIGLINQLVEAFNLIQSLIGM-IGGIRHSP 321
               I      G S  +      +   K    G ++     F LI S   + + GI+   
Sbjct: 293 RKTFIPSMRSMGESETTPSSKFQHSKGK---SGEVSSSESTFPLILSAKRLRLQGIKFRL 352

Query: 322 SFETDSSPKFPTASELKRFKVKFKLPSITMNTFSEVILRNLLAFEVEAKLNPPCLSHYVK 381
             + +S        ++K  K K ++P + ++ F   I  N +AFE     +   ++ YV 
Sbjct: 353 RSDAES------ILDIKLKKNKLQIPLLRLDGFISSIFLNCVAFEQFYTESTNDITSYVV 412

Query: 382 LMSGLIVTANDVTILKQAGIIAGGRSGDEEDVAKLFKELGDVAVKVSSDDNESSSNFDTR 441
            M  L+    D T L     I     G+E +V++ FK +    V            FDTR
Sbjct: 413 FMGCLLNDQEDATFLNNDKRIIENYFGNENEVSQFFKTICKDVV------------FDTR 463

Query: 442 MNFELNIMEKINEYYENRWRVKAERFGKRYICPVFQALLILVVVVLILI 483
            ++  N+ E +NEY    +      F   +    + AL    VV +IL+
Sbjct: 473 RSYLRNVFEGVNEYTSKTYNGVWAGFRHTHFESPWTALSSFAVVFVILL 463

BLAST of Tan0009823 vs. TAIR 10
Match: AT3G47250.2 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 70.5 bits (171), Expect = 4.4e-12
Identity = 112/469 (23.88%), Postives = 192/469 (40.94%), Query Frame = 0

Query: 22  LQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHELKPELQKMEMLK---LESAKKA 81
           L+ A +    IF++PDS+ + NP+AY P  + +GP+H  +  LQ ++  K   LE     
Sbjct: 53  LESAGKASCCIFRIPDSLAEVNPKAYKPKVVSIGPYHYGENHLQMIQQHKFRFLELFVDR 112

Query: 82  KEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRILIDGLFLLRLLEISPDDA 141
             K  + E   +   +  L+ +IRA Y+    +E   L   +++DG F+L LL I     
Sbjct: 113 ATKKGMDE-NVLYAAVGALQHKIRASYSEELRIEKSELVSMMILDGCFILMLLLI----- 172

Query: 142 KEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNITMTQNEIVKDLLKLENQIPIQILKR 201
               +K   + N+      P+F    +  S++            DLL LENQ+P  +L+ 
Sbjct: 173 --VSRKIDLDMNK-----DPIFTIPWILASIQ-----------SDLLLLENQVPFFVLRT 232

Query: 202 VLPRNLV----ENLHLLFYKFCVSVSPLAVYSGDDKDNSSELLEVFDQSQHLLHFLYDLL 261
           +  ++ +    +   + F  F +S+     Y    +D           ++HLL    DL 
Sbjct: 233 IFDKSGIGSPGDLNRMAFSFFNLSIDKPDTYWAKHRDRG---------AKHLL----DLN 292

Query: 262 FYQRITVYLEYGISLKSLHVDLLNMIAKILGIGLINQLVEAFNLIQSLIGM-IGGIRHSP 321
               I      G S  +      +   K    G ++     F LI S   + + GI+   
Sbjct: 293 RKTFIPSMRSMGESETTPSSKFQHSKGK---SGEVSSSESTFPLILSAKRLRLQGIKFRL 352

Query: 322 SFETDSSPKFPTASELKRFKVKFKLPSITMNTFSEVILRNLLAFEVEAKLNPPCLSHYVK 381
             + +S        ++K  K K ++P + ++ F   I  N +AFE     +   ++ YV 
Sbjct: 353 RSDAES------ILDIKLKKNKLQIPLLRLDGFISSIFLNCVAFEQFYTESTNDITSYVV 412

Query: 382 LMSGLIVTANDVTILKQAGIIAGGRSGDEEDVAKLFKELGDVAVKVSSDDNESSSNFDTR 441
            M  L+    D T L     I     G+E +V++ FK +    V            FDTR
Sbjct: 413 FMGCLLNDQEDATFLNNDKRIIENYFGNENEVSQFFKTICKDVV------------FDTR 463

Query: 442 MNFELNIMEKINEYYENRWRVKAERFGKRYICPVFQALLILVVVVLILI 483
            ++  N+ E +NEY    +      F   +    + AL    VV +IL+
Sbjct: 473 RSYLRNVFEGVNEYTSKTYNGVWAGFRHTHFESPWTALSSFAVVFVILL 463

BLAST of Tan0009823 vs. TAIR 10
Match: AT3G47250.3 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 70.5 bits (171), Expect = 4.4e-12
Identity = 112/469 (23.88%), Postives = 192/469 (40.94%), Query Frame = 0

Query: 22  LQLALETPTSIFKLPDSVTKDNPQAYSPSRLFLGPFHELKPELQKMEMLK---LESAKKA 81
           L+ A +    IF++PDS+ + NP+AY P  + +GP+H  +  LQ ++  K   LE     
Sbjct: 53  LESAGKASCCIFRIPDSLAEVNPKAYKPKVVSIGPYHYGENHLQMIQQHKFRFLELFVDR 112

Query: 82  KEKFQLPEFTQMVDELKKLEAEIRACYNGNFDVESEALAWRILIDGLFLLRLLEISPDDA 141
             K  + E   +   +  L+ +IRA Y+    +E   L   +++DG F+L LL I     
Sbjct: 113 ATKKGMDE-NVLYAAVGALQHKIRASYSEELRIEKSELVSMMILDGCFILMLLLI----- 172

Query: 142 KEKEKKGKKNSNRPYGIDSPVFNEQQLYRSLEGNITMTQNEIVKDLLKLENQIPIQILKR 201
               +K   + N+      P+F    +  S++            DLL LENQ+P  +L+ 
Sbjct: 173 --VSRKIDLDMNK-----DPIFTIPWILASIQ-----------SDLLLLENQVPFFVLRT 232

Query: 202 VLPRNLV----ENLHLLFYKFCVSVSPLAVYSGDDKDNSSELLEVFDQSQHLLHFLYDLL 261
           +  ++ +    +   + F  F +S+     Y    +D           ++HLL    DL 
Sbjct: 233 IFDKSGIGSPGDLNRMAFSFFNLSIDKPDTYWAKHRDRG---------AKHLL----DLN 292

Query: 262 FYQRITVYLEYGISLKSLHVDLLNMIAKILGIGLINQLVEAFNLIQSLIGM-IGGIRHSP 321
               I      G S  +      +   K    G ++     F LI S   + + GI+   
Sbjct: 293 RKTFIPSMRSMGESETTPSSKFQHSKGK---SGEVSSSESTFPLILSAKRLRLQGIKFRL 352

Query: 322 SFETDSSPKFPTASELKRFKVKFKLPSITMNTFSEVILRNLLAFEVEAKLNPPCLSHYVK 381
             + +S        ++K  K K ++P + ++ F   I  N +AFE     +   ++ YV 
Sbjct: 353 RSDAES------ILDIKLKKNKLQIPLLRLDGFISSIFLNCVAFEQFYTESTNDITSYVV 412

Query: 382 LMSGLIVTANDVTILKQAGIIAGGRSGDEEDVAKLFKELGDVAVKVSSDDNESSSNFDTR 441
            M  L+    D T L     I     G+E +V++ FK +    V            FDTR
Sbjct: 413 FMGCLLNDQEDATFLNNDKRIIENYFGNENEVSQFFKTICKDVV------------FDTR 463

Query: 442 MNFELNIMEKINEYYENRWRVKAERFGKRYICPVFQALLILVVVVLILI 483
            ++  N+ E +NEY    +      F   +    + AL    VV +IL+
Sbjct: 473 RSYLRNVFEGVNEYTSKTYNGVWAGFRHTHFESPWTALSSFAVVFVILL 463