Tan0009812 (gene) Snake gourd v1

Overview
NameTan0009812
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionAB hydrolase-1 domain-containing protein
LocationLG09: 65493862 .. 65497786 (+)
RNA-Seq ExpressionTan0009812
SyntenyTan0009812
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGTTAAGCTCGAAAATGGGTCGTCGTCGTCGTTGTCAACAGCCGTAAATGCGAAGATTATTGGTTCAGGGAGGGAAGCCATGGTTTTGGCTCATGGGTTCGGAACAAACCAGTCATTATGGGACAAAATCCTTCCAAAGCTGAGCCAGTACTACCGAGTCTTGGTGTTTGATTGGAGCTTTTCGGGTTCCATTAAAGATCCCAACTTGTTTGATCCTCAAAAATACTCTTCCTATCATGGTTTTGCTGAAGATCTCATCACTCTTCTTGATGAAATTGGCCTCACTTCCACCATCTTCATTGGCCACTCCATGTCTGGCCTTATTGGATGCCTTGCTTACACTCAAAGACCTGACCTTTTTCAGACACTCATTCTTCTCTGTTCTTCTCCCAGGTATTCTTTATTATATATTATTATTAATTTCCTCCAAACACAAACAACAACAAATTTACCACCCATCTTTTTGCAACCTATTTAATTGTCCATCTATGATTTGTCACGTCACAATTCTTTTTTTTATTTTTTTTTATATTATTCTTTTTGTTGCCTTAAAAGAGTGAGCTGCAAAAAGATGGATTGCATCCAAATATTGTTCCTCGATTGTTTGATCCTTATCTACGTTGTGTTGTGTTGTTTTAATTTTTTCTTTTTCTGGATTTTTTTTTCAAATAAAATTGGATAAATTTGGGAGCTGTGTGTTCTCAGCCTCACAACATTTTGGAGTGAGTCCAAACCAATTCCACTAGAGGATATGGTGGCCATATATGTATATTGGGTGTTGATAAAGTGGTGAAATAAATACTTCTTCACTCAAATTTTTAATCTTGAATAGAGATCTTAATTCGAATTAGATCTTGGAGTCGAGTTTCATGAAATATAGTCAATGTTTTTAAAGCTTATCTATGGATTTACTTATCTAGAAAATTTTAACACTGAAAATTTTTTTTTGTTAAGAATTTAATCTATCTCTAAAAAGTCTTTTGAATTTTTGAACTTATATCTAATAAATCTTTAAATTTTCTAATAAGCTGACTTTAAACTATTAACTTTAGATTTGATAGGTCTTTAACGTATTAAAAAAATTAAATTAATAGATCTATTATATATAGGGCCCTCAATTTTGGGTTTGAAAGTTTCATGAATTTTAAAAAAGATTGAATAGGTCAAAGATCTATTAGACATAAAGTTGAAAGTCTAAAATTTATTAGACAGGTTAGGAACCTATCAAACTAAAAGTTCAAAGAGTAGAAAACATTCTCTAAAATTCAAGAACCAATAAAAATAATTCTTAAACAAAAAAGGAAATGATTGTTAGAAATAACCCATAAAACAGTTTGTTTATTCTTCTAGTACAGCTTTGCTTAGCTCATTTATACCCCACTCATAATTAAACACATGCATGACTTCTTATATAGTAGCTATGGAAATTATATTCTTATTTAACTTCTTGTGGAGTGGGTTTTCCTTTCGGCATAAATATTTGTCATCGCATCTCTTGTTTGTTATATCATATTTCTAATTAAAACTAAACATTTATATTCAAAAGGAGGGGAAGATTTGCATGGATCATTTTAAATATTATTATATGTCAATTAGGTATTGGAATTTGGCTTATCTATTTCATTGTCTCTACCATTTTCTCTCTTTTTCTTACTCTTTTGTCAATTTTGCTATCACTGGAGGGGAAAACTTCGTCTTAAATGGAAATTTGTCAGGTTAATCTTTAAGCTAAATGGCACAAACCACCTCTAAACTTTGAGGTTTGTTATAATCACACTATTTTTAATTTAATCAATCAGTTCTCATATTTTGTTTGTATTGAAATTACATCCTTAAATTTTAATTTGATCAATAAGCGTCATGTGCTTTTGTTAAGTGTTGCGACCAATAGACTTTACATGTGTGACTTATACATTAGTAGCTAGTCTAGTAAGGGAGGGTGAACTTGTCATTGAGGATCTTACGATGACGACCTCTATTTTTGAGCTGTACGGATTGGACAACCCCATCATCGACTAATTTGGAGAAGGAAGTTTCAACTATGGTGATTGAAGTCTTCTGGCGTTTCTTAATTCCTTACTATAAAAAAAATTGAAAAATTACACGATCCAAATAACACCCACTAAATTGAAATTTCCTACGCTTCTTTTTCTAAAACATAATTTTTTTTCCTTTTATCTTTTTTCCAGATCTATCGATAACATGTTTGATCTTCCCTACTAACATGTGAGTCACAGTCACACATGTGAAGTCCAAATAAACGGTAATTTAATAGCAACTAGAAGGAGATTGATTGCAACACTTAACAAAATACAAGGAATTGATTGGTCAAATTAAAAATTCATGGGTATGATTACAACACTTAACAAAATACAAGGGATTGATTGGTTAAATTAAAAATTTAAGGATGTGATTATAACCAAGATGTCGACGGAAATGTCGATGTCTCAATTTTATAGAAATGTGGATGGAAATATCGATAAAATGTTGATATCGATGGATATTTCTAGAAAAAATTTAAAAATAAAAAATTAATATAGTTAAAATACTTGTTATAAATAATATAGTTACATTTTGCTGCTATTTCTTTAATATTACATCAATTTTGTATGATATAATGGAAATATCGATTCACTCGATGACGAACCCATGGACATATGGAAAGGTCGACATGTCAACGAAAATTTAATACGATGATTGTAACAAAATCTCATAGTTCAAGAGTGGTTTGTACAATTTTTCCTAATCCCTATCATTCATTATTATATAATTTTTTTAAAAAAAAAAATAGTTTAGTAACAAAACTTCATAGTTCAATGCTGGAGATTCAAACCTTTAATTTTGTGGTCGATGATATATACACCTTAACTATTTAAACTATGCTCCTATTGATAATATTAAAAAAAAAAACATTTTAAATGTAATCACTTATATTTTTTTTTTTTTTAAACAACATATTGGGTGGGTTTTTTTTTTTTTTTGGTTTTCAATATGACGTAAGATTTTTACAGTTAGTTTCTACTTTTAAATTTGTCACGAAAATGTTCCAAAAATATTATCCTGTTTTTATATTATTACCTTTGTACCTCTCCCTATATTATAATCATGAATTGTACTAATGAGTTTATTTCATGTTCGTAGAGTTAGCTTGATGGGATTTGGGAGAAATTGAACTAACTTTAGCTAATCAAAAGGGCTTTTAAAACTCTTAATTATATTAAATTAATTGCCAAAAGCATTCTTAAAATTTTCAAATATTTAGTAAGCAGTTAAAAATACACCTAGACTAAAAAGTAAAGTGTTTTTTCTTCCAAAACTGTTTCATTTAAAAAGCACTTGTATAGAAAAGGATTCCGAACAGAATACACTTTTAATCTTACCTAATTAAAATGGTTTGATTCAGTGAAAGACAGATCCAATTTAAACAGCAAAAGGAGGCTCAAAGCTATATTCTTTCTAACAACAAGTTGACCATATTGTGTGTTGTTAGGTATATAAACACAGAAGACTATGAAGGTGGTTTCGAGGTATCAGACGTCGAGCAAATCATAGCAAATATTGAATCCAACTACGAAAATTGGGCCACAAACTTTCCTTCTCTGGTTGTGGACGAAGCCGATCCTCTCTCAGTAAGAAAGTTGCAGAAATGCTTCCATGAAATGAGGCCTGAAGTTGCATTACCCTTAGCCAAGACTGTCTTCTTCGTGGACGAGAGAGACATTCTCGAAAAGGTCGACATTCCCTGCACCATCATACAGACGAAAACCGACGTCGTCGTCCCTGCCTCGGTTGCTCTCTTCATGCAGAAGAAAATTAAGGGCAGTTGCACAGTGCAAGTTATAAACACTGATGGTCATTTCCCTCACTTGACTGCACACCTTGAGTTGCTTCAAGTTCTTGGAAAAATCTTGGGCTTTTGA

mRNA sequence

ATGGTTGTTAAGCTCGAAAATGGGTCGTCGTCGTCGTTGTCAACAGCCGTAAATGCGAAGATTATTGGTTCAGGGAGGGAAGCCATGGTTTTGGCTCATGGGTTCGGAACAAACCAGTCATTATGGGACAAAATCCTTCCAAAGCTGAGCCAGTACTACCGAGTCTTGGTGTTTGATTGGAGCTTTTCGGGTTCCATTAAAGATCCCAACTTGTTTGATCCTCAAAAATACTCTTCCTATCATGGTTTTGCTGAAGATCTCATCACTCTTCTTGATGAAATTGGCCTCACTTCCACCATCTTCATTGGCCACTCCATGTCTGGCCTTATTGGATGCCTTGCTTACACTCAAAGACCTGACCTTTTTCAGACACTCATTCTTCTCTGTTCTTCTCCCAGGTATATAAACACAGAAGACTATGAAGGTGGTTTCGAGGTATCAGACGTCGAGCAAATCATAGCAAATATTGAATCCAACTACGAAAATTGGGCCACAAACTTTCCTTCTCTGGTTGTGGACGAAGCCGATCCTCTCTCAGTAAGAAAGTTGCAGAAATGCTTCCATGAAATGAGGCCTGAAGTTGCATTACCCTTAGCCAAGACTGTCTTCTTCGTGGACGAGAGAGACATTCTCGAAAAGGTCGACATTCCCTGCACCATCATACAGACGAAAACCGACGTCGTCGTCCCTGCCTCGGTTGCTCTCTTCATGCAGAAGAAAATTAAGGGCAGTTGCACAGTGCAAGTTATAAACACTGATGGTCATTTCCCTCACTTGACTGCACACCTTGAGTTGCTTCAAGTTCTTGGAAAAATCTTGGGCTTTTGA

Coding sequence (CDS)

ATGGTTGTTAAGCTCGAAAATGGGTCGTCGTCGTCGTTGTCAACAGCCGTAAATGCGAAGATTATTGGTTCAGGGAGGGAAGCCATGGTTTTGGCTCATGGGTTCGGAACAAACCAGTCATTATGGGACAAAATCCTTCCAAAGCTGAGCCAGTACTACCGAGTCTTGGTGTTTGATTGGAGCTTTTCGGGTTCCATTAAAGATCCCAACTTGTTTGATCCTCAAAAATACTCTTCCTATCATGGTTTTGCTGAAGATCTCATCACTCTTCTTGATGAAATTGGCCTCACTTCCACCATCTTCATTGGCCACTCCATGTCTGGCCTTATTGGATGCCTTGCTTACACTCAAAGACCTGACCTTTTTCAGACACTCATTCTTCTCTGTTCTTCTCCCAGGTATATAAACACAGAAGACTATGAAGGTGGTTTCGAGGTATCAGACGTCGAGCAAATCATAGCAAATATTGAATCCAACTACGAAAATTGGGCCACAAACTTTCCTTCTCTGGTTGTGGACGAAGCCGATCCTCTCTCAGTAAGAAAGTTGCAGAAATGCTTCCATGAAATGAGGCCTGAAGTTGCATTACCCTTAGCCAAGACTGTCTTCTTCGTGGACGAGAGAGACATTCTCGAAAAGGTCGACATTCCCTGCACCATCATACAGACGAAAACCGACGTCGTCGTCCCTGCCTCGGTTGCTCTCTTCATGCAGAAGAAAATTAAGGGCAGTTGCACAGTGCAAGTTATAAACACTGATGGTCATTTCCCTCACTTGACTGCACACCTTGAGTTGCTTCAAGTTCTTGGAAAAATCTTGGGCTTTTGA

Protein sequence

MVVKLENGSSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDWSFSGSIKDPNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPDLFQTLILLCSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSVRKLQKCFHEMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKKIKGSCTVQVINTDGHFPHLTAHLELLQVLGKILGF
Homology
BLAST of Tan0009812 vs. ExPASy Swiss-Prot
Match: J9U5U9 (Probable strigolactone esterase DAD2 OS=Petunia hybrida OX=4102 GN=DAD2 PE=1 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 2.6e-59
Identity = 110/262 (41.98%), Postives = 172/262 (65.65%), Query Frame = 0

Query: 12  SLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDWSFSGSIKDPNL 71
           +L  A+N +++GSG   +VLAHGFGT+QS W++ILP   + YRV+++D   +GS+ +P+ 
Sbjct: 4   TLLDALNVRVVGSGERVLVLAHGFGTDQSAWNRILPFFLRDYRVVLYDLVCAGSV-NPDF 63

Query: 72  FDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPDLFQTLILLCSS 131
           FD ++Y++   + +DL+ +LD +G+    ++GHS+S +IG LA  +RP+LF  LIL+ +S
Sbjct: 64  FDFRRYTTLDPYVDDLLHILDALGIDCCAYVGHSVSAMIGILASIRRPELFSKLILIGAS 123

Query: 132 PRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSVRKLQKCFHEMR 191
           PR++N EDY GGFE  ++E++ + +E+NYE W   F  L V    P +VR+  +    MR
Sbjct: 124 PRFLNDEDYHGGFEQGEIEKVFSAMEANYEAWVNGFAPLAVGADVPAAVREFSRTLFNMR 183

Query: 192 PEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKKIKGSCTVQVIN 251
           P++ L +++TVF  D R +L  V +PC I QT  D  VPASVA +++  + G  TV  +N
Sbjct: 184 PDITLFVSRTVFNSDMRGVLGLVKVPCHIFQTARDHSVPASVATYLKNHLGGKNTVHWLN 243

Query: 252 TDGHFPHLTAHLELLQVLGKIL 274
            +GH PHL+A   L Q L + L
Sbjct: 244 IEGHLPHLSAPTLLAQELRRAL 264

BLAST of Tan0009812 vs. ExPASy Swiss-Prot
Match: Q10QA5 (Strigolactone esterase D14 OS=Oryza sativa subsp. japonica OX=39947 GN=D14 PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 7.5e-59
Identity = 106/265 (40.00%), Postives = 173/265 (65.28%), Query Frame = 0

Query: 9   SSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDWSFSGSIKD 68
           S + L   +N +++GSG   +VL+HGFGT+QS W ++LP L++ +RV+++D   +GS+ +
Sbjct: 52  SGAKLLQILNVRVVGSGERVVVLSHGFGTDQSAWSRVLPYLTRDHRVVLYDLVCAGSV-N 111

Query: 69  PNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPDLFQTLILL 128
           P+ FD ++Y +   + +DL+ +LD + +    F+GHS+S +IG LA  +RPDLF  L+L+
Sbjct: 112 PDHFDFRRYDNLDAYVDDLLAILDALRIPRCAFVGHSVSAMIGILASIRRPDLFAKLVLI 171

Query: 129 CSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSVRKLQKCFH 188
            +SPR++N  DY GGFE+ +++Q+   + +NY  WAT +  L V    P +V++  +   
Sbjct: 172 GASPRFLNDSDYHGGFELEEIQQVFDAMGANYSAWATGYAPLAVGADVPAAVQEFSRTLF 231

Query: 189 EMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKKIKGSCTVQ 248
            MRP+++L + +TVF  D R +L  V  PC ++QT  DV VPASVA +++  + G  TV+
Sbjct: 232 NMRPDISLHVCQTVFKTDLRGVLGMVRAPCVVVQTTRDVSVPASVAAYLKAHLGGRTTVE 291

Query: 249 VINTDGHFPHLTAHLELLQVLGKIL 274
            + T+GH PHL+A   L QVL + L
Sbjct: 292 FLQTEGHLPHLSAPSLLAQVLRRAL 315

BLAST of Tan0009812 vs. ExPASy Swiss-Prot
Match: Q9SQR3 (Strigolactone esterase D14 OS=Arabidopsis thaliana OX=3702 GN=D14 PE=1 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 6.3e-58
Identity = 106/265 (40.00%), Postives = 173/265 (65.28%), Query Frame = 0

Query: 9   SSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDWSFSGSIKD 68
           S  ++  A+N +++G+G   + LAHGFGT+QS W  ILP  +Q YRV+++D   +GS+ +
Sbjct: 2   SQHNILEALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSV-N 61

Query: 69  PNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPDLFQTLILL 128
           P+ FD  +Y++   + +DL+ ++D +G+ +  ++GHS+S +IG +A  +RP+LF  LIL+
Sbjct: 62  PDYFDFNRYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILI 121

Query: 129 CSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSVRKLQKCFH 188
             SPR++N EDY GGFE  ++E++ + +E+NYE W   F  L V    P +VR+  +   
Sbjct: 122 GFSPRFLNDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLF 181

Query: 189 EMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKKIKGSCTVQ 248
            MRP+++L +++TVF  D R +L  V +P  +IQT  DV VPASVA +++  + G  TV+
Sbjct: 182 NMRPDISLFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVE 241

Query: 249 VINTDGHFPHLTAHLELLQVLGKIL 274
            + T+GH P L+A  +L Q L + L
Sbjct: 242 TLKTEGHLPQLSAPAQLAQFLRRAL 265

BLAST of Tan0009812 vs. ExPASy Swiss-Prot
Match: A0A109QYD3 (Strigolactone esterase RMS3 OS=Pisum sativum OX=3888 GN=RMS3 PE=1 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.2e-56
Identity = 104/247 (42.11%), Postives = 168/247 (68.02%), Query Frame = 0

Query: 16  AVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDWSFSGSIKDPNLFDPQ 75
           A N ++ GSG + +V AHGFGT+QS W ++LP  ++ Y+V+++D   +GS+ +P+ FD +
Sbjct: 8   AFNVRVEGSGDKYLVFAHGFGTDQSAWQRVLPYFTRSYKVILYDLVCAGSV-NPDHFDFR 67

Query: 76  KYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPDLFQTLILLCSSPRYI 135
           +Y++   + +DL+ +LD + +T   ++GHS+S + G LA  +RP+LF  LIL+ +SPR++
Sbjct: 68  RYTTLDAYVDDLLNILDSLHVTRCAYVGHSISAMTGMLASIRRPELFSKLILIGASPRFL 127

Query: 136 NT-EDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSVRKLQKCFHEMRPEV 195
           N  E+Y GGFE  ++E + + +E+NYE W   F  L V    P +VR+  +    MRP++
Sbjct: 128 NDGENYHGGFEQGEIEHVFSAMEANYEAWVNGFAPLAVGADVPTAVREFSRTLFNMRPDI 187

Query: 196 ALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKKIKGSCTVQVINTDG 255
           +L +++TVF  D R IL  V++PC I+QT  D+ VPASVA +M++ I G  TVQ ++T+G
Sbjct: 188 SLFVSRTVFNSDLRGILGLVNVPCCIMQTARDMSVPASVATYMKEHIGGKSTVQWLDTEG 247

Query: 256 HFPHLTA 262
           H PHL+A
Sbjct: 248 HLPHLSA 253

BLAST of Tan0009812 vs. ExPASy Swiss-Prot
Match: Q9SZU7 (Probable esterase KAI2 OS=Arabidopsis thaliana OX=3702 GN=KAI2 PE=1 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 4.3e-54
Identity = 101/255 (39.61%), Postives = 161/255 (63.14%), Query Frame = 0

Query: 16  AVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDWSFSGSIKDPNLFDPQ 75
           A N K+IGSG   +VL HGFGT+QS+W  ++P L   YRV+++D +      +P+ FD  
Sbjct: 7   AHNVKVIGSGEATIVLGHGFGTDQSVWKHLVPHLVDDYRVVLYD-NMGAGTTNPDYFDFD 66

Query: 76  KYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPDLFQTLILLCSSPRYI 135
           +YS+  G++ DLI +L+++ + S IF+GHS+S +IG LA   RPDLF  ++++ +SPRY+
Sbjct: 67  RYSNLEGYSFDLIAILEDLKIESCIFVGHSVSAMIGVLASLNRPDLFSKIVMISASPRYV 126

Query: 136 NTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVV-DEADPLSVRKLQKCFHEMRPEV 195
           N  DY+GGFE  D+ Q+   I SNY+ W   F  L V  + D ++V++  +    MRP++
Sbjct: 127 NDVDYQGGFEQEDLNQLFEAIRSNYKAWCLGFAPLAVGGDMDSIAVQEFSRTLFNMRPDI 186

Query: 196 ALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKKIKGSCTVQVINTDG 255
           AL + +T+F  D R IL  V +PC I+Q+  D+ VP  V+ ++   +     V+VI +DG
Sbjct: 187 ALSVGQTIFQSDMRQILPFVTVPCHILQSVKDLAVPVVVSEYLHANLGCESVVEVIPSDG 246

Query: 256 HFPHLTAHLELLQVL 270
           H P L++   ++ V+
Sbjct: 247 HLPQLSSPDSVIPVI 260

BLAST of Tan0009812 vs. NCBI nr
Match: XP_023002408.1 (probable strigolactone esterase DAD2 [Cucurbita maxima])

HSP 1 Score: 471.5 bits (1212), Expect = 5.1e-129
Identity = 230/275 (83.64%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   MVVKLENGSSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDW 60
           MVV L+ G     STAVNAKIIGSG E MVLAHGFG NQSLWDKILPKL+ +YRVL+FDW
Sbjct: 1   MVVMLQTG----FSTAVNAKIIGSGSEVMVLAHGFGGNQSLWDKILPKLTHHYRVLLFDW 60

Query: 61  SFSGSIKDPNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPD 120
           +F+GSIKDPNLFDP KYSSY GFAEDLI +LDE+GLTSTIF+GHSMSGLIGC+AYT RPD
Sbjct: 61  TFAGSIKDPNLFDPHKYSSYSGFAEDLIKILDELGLTSTIFLGHSMSGLIGCVAYTIRPD 120

Query: 121 LFQTLILLCSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSV 180
           LFQ L+LLCSSPRYINTE YEGGFE SD+EQIIA+IESNY+NWAT+FPSLVVDEADP+SV
Sbjct: 121 LFQRLVLLCSSPRYINTEGYEGGFEQSDIEQIIASIESNYQNWATHFPSLVVDEADPVSV 180

Query: 181 RKLQKCFHEMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKK 240
            + QKC HEMRPEVALPLAKTVF VDERDILEKVDIPCTI+QTKTD+VVPASVA+FMQ K
Sbjct: 181 SRFQKCLHEMRPEVALPLAKTVFGVDERDILEKVDIPCTIVQTKTDIVVPASVAVFMQNK 240

Query: 241 IKGSCTVQVINTDGHFPHLTAHLELLQVLGKILGF 276
           IKG CTVQVINTDGHFPHLTAHLELLQVLG ILGF
Sbjct: 241 IKGCCTVQVINTDGHFPHLTAHLELLQVLGTILGF 271

BLAST of Tan0009812 vs. NCBI nr
Match: KAG6587947.1 (putative strigolactone esterase DAD2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021836.1 putative strigolactone esterase DAD2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 471.5 bits (1212), Expect = 5.1e-129
Identity = 228/275 (82.91%), Postives = 251/275 (91.27%), Query Frame = 0

Query: 1   MVVKLENGSSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDW 60
           MVV L+ G     STAVNAKIIGSG E MVLAHGFG NQSLWDKILPKL+ +YRVL+FDW
Sbjct: 1   MVVMLQTG----FSTAVNAKIIGSGSEVMVLAHGFGGNQSLWDKILPKLTHHYRVLLFDW 60

Query: 61  SFSGSIKDPNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPD 120
           +F+GSIKDPNLFDP KYSSY GFAEDLI +LDE+GLTSTIF+GHSMSGLIGC+AYT+RPD
Sbjct: 61  TFAGSIKDPNLFDPHKYSSYSGFAEDLIRILDELGLTSTIFLGHSMSGLIGCVAYTKRPD 120

Query: 121 LFQTLILLCSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSV 180
           LFQ L+LLCSSPRYINTE YEGGFE SD++QIIA+IESNY+NWAT+FPSLVVDEADP+SV
Sbjct: 121 LFQRLVLLCSSPRYINTEGYEGGFEKSDIDQIIASIESNYQNWATHFPSLVVDEADPVSV 180

Query: 181 RKLQKCFHEMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKK 240
            + QKC HEMRPEVALPLAKTVF VDERDILEKVDIPCTI+QTKTD+VVPASVA+FMQ K
Sbjct: 181 SRFQKCLHEMRPEVALPLAKTVFGVDERDILEKVDIPCTIVQTKTDIVVPASVAVFMQNK 240

Query: 241 IKGSCTVQVINTDGHFPHLTAHLELLQVLGKILGF 276
           IKG CTVQVINTDGHFPHLTAHLELLQVLG +LGF
Sbjct: 241 IKGCCTVQVINTDGHFPHLTAHLELLQVLGTVLGF 271

BLAST of Tan0009812 vs. NCBI nr
Match: XP_023530785.1 (probable strigolactone esterase DAD2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 470.7 bits (1210), Expect = 8.6e-129
Identity = 228/275 (82.91%), Postives = 252/275 (91.64%), Query Frame = 0

Query: 1   MVVKLENGSSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDW 60
           MVV L+ G     STAVNAKIIGSG E MVLAHGFG NQSLWDKILP+L+++YRVL+FDW
Sbjct: 1   MVVMLQTG----FSTAVNAKIIGSGSEVMVLAHGFGGNQSLWDKILPELTRHYRVLLFDW 60

Query: 61  SFSGSIKDPNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPD 120
           +F+GSIKDPNLFDP KYSSY GFAEDLI +LDE+GLTSTIF+GHSMSGLIGC+AYT+RPD
Sbjct: 61  TFAGSIKDPNLFDPHKYSSYCGFAEDLIKILDELGLTSTIFLGHSMSGLIGCVAYTKRPD 120

Query: 121 LFQTLILLCSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSV 180
           LFQ L+LLCSSPRYINTE YEGGFE SD+EQIIA+IESNY+NWAT+FPSLVVDEADP+SV
Sbjct: 121 LFQRLVLLCSSPRYINTEGYEGGFEKSDIEQIIASIESNYQNWATHFPSLVVDEADPVSV 180

Query: 181 RKLQKCFHEMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKK 240
            + QKC HEMRPEVALPLAKTVF VDERDILEKVDIPCTI+QTKTD+VVPASVA+FMQ K
Sbjct: 181 SRFQKCLHEMRPEVALPLAKTVFGVDERDILEKVDIPCTIVQTKTDIVVPASVAVFMQNK 240

Query: 241 IKGSCTVQVINTDGHFPHLTAHLELLQVLGKILGF 276
           IKG CTVQVINTDGHFPHLTAHLELLQVLG +LGF
Sbjct: 241 IKGCCTVQVINTDGHFPHLTAHLELLQVLGTVLGF 271

BLAST of Tan0009812 vs. NCBI nr
Match: XP_022933803.1 (probable strigolactone esterase DAD2 [Cucurbita moschata])

HSP 1 Score: 469.2 bits (1206), Expect = 2.5e-128
Identity = 227/275 (82.55%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   MVVKLENGSSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDW 60
           MVV L+ G     STAVNAKIIGSG E MVLAHGFG NQSLWDKILPKL+ +YRVL+FDW
Sbjct: 1   MVVMLQTG----FSTAVNAKIIGSGSEVMVLAHGFGGNQSLWDKILPKLTHHYRVLLFDW 60

Query: 61  SFSGSIKDPNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPD 120
           +F+GSIKDPNLFDP KYSSY GFAEDLI +LDE+GLTSTIF+GHSMSGLIGC+AYT+RPD
Sbjct: 61  TFAGSIKDPNLFDPHKYSSYSGFAEDLIKILDELGLTSTIFLGHSMSGLIGCVAYTKRPD 120

Query: 121 LFQTLILLCSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSV 180
           LFQ L+LLCSSPRYINTE YEGGFE SD++QIIA+IESNY+NWAT+FPSLVVDEADP+SV
Sbjct: 121 LFQRLVLLCSSPRYINTEGYEGGFEKSDIDQIIASIESNYQNWATHFPSLVVDEADPVSV 180

Query: 181 RKLQKCFHEMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKK 240
            + QKC HEMRPEVALPLAKTVF VDERDILE VDIPCTI+QTKTD+VVPASVA+FMQ K
Sbjct: 181 SRFQKCLHEMRPEVALPLAKTVFGVDERDILEMVDIPCTIVQTKTDIVVPASVAVFMQNK 240

Query: 241 IKGSCTVQVINTDGHFPHLTAHLELLQVLGKILGF 276
           IKG CTVQVINTDGHFPHLTAHLELLQVLG +LGF
Sbjct: 241 IKGCCTVQVINTDGHFPHLTAHLELLQVLGTVLGF 271

BLAST of Tan0009812 vs. NCBI nr
Match: XP_022144907.1 (probable strigolactone esterase DAD2 [Momordica charantia])

HSP 1 Score: 457.2 bits (1175), Expect = 9.9e-125
Identity = 221/275 (80.36%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   MVVKLENGSSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDW 60
           MVV +ENG    LS A+NA+I+GSG EAMVLAHGFGTNQSLWDKILPKL Q+YRV+VFDW
Sbjct: 1   MVVMIENG---LLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDW 60

Query: 61  SFSGSIKDPNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPD 120
           SFSG+IKDPNLFDPQKY SY+GFA+DLI LL+E+GL+STIFIGHSMSGLIGC+AYTQ P 
Sbjct: 61  SFSGAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPH 120

Query: 121 LFQTLILLCSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSV 180
           LFQ LILLC+SPRYINTEDYEGGF+ SD+EQIIANIESNYENWATNF SLVVDE+DP+S+
Sbjct: 121 LFQRLILLCASPRYINTEDYEGGFDNSDIEQIIANIESNYENWATNFSSLVVDESDPVSL 180

Query: 181 RKLQKCFHEMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKK 240
            + QKC HEMRPEVA+ LAKTVF  DERD+LEKVDIPCTI+QTKTD+VVPASVA +MQKK
Sbjct: 181 GRFQKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKK 240

Query: 241 IKGSCTVQVINTDGHFPHLTAHLELLQVLGKILGF 276
           IKGS TV+VI+T GHFPHLTAHL+LLQVLG +LGF
Sbjct: 241 IKGSSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 272

BLAST of Tan0009812 vs. ExPASy TrEMBL
Match: A0A6J1KTG9 (probable strigolactone esterase DAD2 OS=Cucurbita maxima OX=3661 GN=LOC111496258 PE=4 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 2.5e-129
Identity = 230/275 (83.64%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   MVVKLENGSSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDW 60
           MVV L+ G     STAVNAKIIGSG E MVLAHGFG NQSLWDKILPKL+ +YRVL+FDW
Sbjct: 1   MVVMLQTG----FSTAVNAKIIGSGSEVMVLAHGFGGNQSLWDKILPKLTHHYRVLLFDW 60

Query: 61  SFSGSIKDPNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPD 120
           +F+GSIKDPNLFDP KYSSY GFAEDLI +LDE+GLTSTIF+GHSMSGLIGC+AYT RPD
Sbjct: 61  TFAGSIKDPNLFDPHKYSSYSGFAEDLIKILDELGLTSTIFLGHSMSGLIGCVAYTIRPD 120

Query: 121 LFQTLILLCSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSV 180
           LFQ L+LLCSSPRYINTE YEGGFE SD+EQIIA+IESNY+NWAT+FPSLVVDEADP+SV
Sbjct: 121 LFQRLVLLCSSPRYINTEGYEGGFEQSDIEQIIASIESNYQNWATHFPSLVVDEADPVSV 180

Query: 181 RKLQKCFHEMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKK 240
            + QKC HEMRPEVALPLAKTVF VDERDILEKVDIPCTI+QTKTD+VVPASVA+FMQ K
Sbjct: 181 SRFQKCLHEMRPEVALPLAKTVFGVDERDILEKVDIPCTIVQTKTDIVVPASVAVFMQNK 240

Query: 241 IKGSCTVQVINTDGHFPHLTAHLELLQVLGKILGF 276
           IKG CTVQVINTDGHFPHLTAHLELLQVLG ILGF
Sbjct: 241 IKGCCTVQVINTDGHFPHLTAHLELLQVLGTILGF 271

BLAST of Tan0009812 vs. ExPASy TrEMBL
Match: A0A6J1F5V4 (probable strigolactone esterase DAD2 OS=Cucurbita moschata OX=3662 GN=LOC111441110 PE=4 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.2e-128
Identity = 227/275 (82.55%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   MVVKLENGSSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDW 60
           MVV L+ G     STAVNAKIIGSG E MVLAHGFG NQSLWDKILPKL+ +YRVL+FDW
Sbjct: 1   MVVMLQTG----FSTAVNAKIIGSGSEVMVLAHGFGGNQSLWDKILPKLTHHYRVLLFDW 60

Query: 61  SFSGSIKDPNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPD 120
           +F+GSIKDPNLFDP KYSSY GFAEDLI +LDE+GLTSTIF+GHSMSGLIGC+AYT+RPD
Sbjct: 61  TFAGSIKDPNLFDPHKYSSYSGFAEDLIKILDELGLTSTIFLGHSMSGLIGCVAYTKRPD 120

Query: 121 LFQTLILLCSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSV 180
           LFQ L+LLCSSPRYINTE YEGGFE SD++QIIA+IESNY+NWAT+FPSLVVDEADP+SV
Sbjct: 121 LFQRLVLLCSSPRYINTEGYEGGFEKSDIDQIIASIESNYQNWATHFPSLVVDEADPVSV 180

Query: 181 RKLQKCFHEMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKK 240
            + QKC HEMRPEVALPLAKTVF VDERDILE VDIPCTI+QTKTD+VVPASVA+FMQ K
Sbjct: 181 SRFQKCLHEMRPEVALPLAKTVFGVDERDILEMVDIPCTIVQTKTDIVVPASVAVFMQNK 240

Query: 241 IKGSCTVQVINTDGHFPHLTAHLELLQVLGKILGF 276
           IKG CTVQVINTDGHFPHLTAHLELLQVLG +LGF
Sbjct: 241 IKGCCTVQVINTDGHFPHLTAHLELLQVLGTVLGF 271

BLAST of Tan0009812 vs. ExPASy TrEMBL
Match: A0A6J1CV13 (probable strigolactone esterase DAD2 OS=Momordica charantia OX=3673 GN=LOC111014472 PE=4 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 4.8e-125
Identity = 221/275 (80.36%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   MVVKLENGSSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDW 60
           MVV +ENG    LS A+NA+I+GSG EAMVLAHGFGTNQSLWDKILPKL Q+YRV+VFDW
Sbjct: 1   MVVMIENG---LLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDW 60

Query: 61  SFSGSIKDPNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPD 120
           SFSG+IKDPNLFDPQKY SY+GFA+DLI LL+E+GL+STIFIGHSMSGLIGC+AYTQ P 
Sbjct: 61  SFSGAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPH 120

Query: 121 LFQTLILLCSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSV 180
           LFQ LILLC+SPRYINTEDYEGGF+ SD+EQIIANIESNYENWATNF SLVVDE+DP+S+
Sbjct: 121 LFQRLILLCASPRYINTEDYEGGFDNSDIEQIIANIESNYENWATNFSSLVVDESDPVSL 180

Query: 181 RKLQKCFHEMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKK 240
            + QKC HEMRPEVA+ LAKTVF  DERD+LEKVDIPCTI+QTKTD+VVPASVA +MQKK
Sbjct: 181 GRFQKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKK 240

Query: 241 IKGSCTVQVINTDGHFPHLTAHLELLQVLGKILGF 276
           IKGS TV+VI+T GHFPHLTAHL+LLQVLG +LGF
Sbjct: 241 IKGSSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 272

BLAST of Tan0009812 vs. ExPASy TrEMBL
Match: A0A6J1JFG1 (probable strigolactone esterase DAD2 OS=Cucurbita maxima OX=3661 GN=LOC111485258 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.4e-124
Identity = 221/275 (80.36%), Postives = 251/275 (91.27%), Query Frame = 0

Query: 1   MVVKLENGSSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDW 60
           MVV +ENG    LSTA+NAK++GSGREAMVLAHGFGTNQSLWD ++ KLSQ YRV+VFDW
Sbjct: 1   MVVMVENG---PLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDW 60

Query: 61  SFSGSIKDPNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPD 120
           +F GSIKDPNL+DPQKYSSY  FAEDLI LLDE+GLTSTIF+GHSMSGLIGC+A T+RPD
Sbjct: 61  TFWGSIKDPNLYDPQKYSSYSAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPD 120

Query: 121 LFQTLILLCSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSV 180
           LFQ LILLCSSPRY+NTEDYEGGF+ SD+EQ+IAN+ESNYENWAT F SLVVDEADPLS+
Sbjct: 121 LFQRLILLCSSPRYLNTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSL 180

Query: 181 RKLQKCFHEMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKK 240
            +L+KC HEMRPEVA+ LAKTVF VDER+ILEKVDIPCTI+ TKTDV  PASVA+FMQK+
Sbjct: 181 IRLRKCLHEMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKTDVAAPASVAIFMQKQ 240

Query: 241 IKGSCTVQVINTDGHFPHLTAHLELLQVLGKILGF 276
           IKGSCTV+VINT+GHFPHLTAHL+LLQVLG+ILGF
Sbjct: 241 IKGSCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272

BLAST of Tan0009812 vs. ExPASy TrEMBL
Match: A0A6J1H9Y6 (probable strigolactone esterase DAD2 OS=Cucurbita moschata OX=3662 GN=LOC111461858 PE=4 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 9.0e-124
Identity = 220/275 (80.00%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   MVVKLENGSSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDW 60
           MVV +ENG    LS A+NAK++GSGREAMVLAHGFGTNQSLWD ++ KLSQ YRV+VFDW
Sbjct: 1   MVVMVENG---PLSMALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDW 60

Query: 61  SFSGSIKDPNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPD 120
           +F GSIKDPNL+DPQKYSSY+ FAEDLI LLDE+GLTSTIF+GHSMSGLIGC+A T+RPD
Sbjct: 61  TFWGSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPD 120

Query: 121 LFQTLILLCSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSV 180
           LFQ LILLCSSPRYINTEDYEGGF+ SD+EQ+IAN+E+NYENWAT F SLVVDEADPLS+
Sbjct: 121 LFQRLILLCSSPRYINTEDYEGGFDQSDIEQMIANMEANYENWATQFSSLVVDEADPLSL 180

Query: 181 RKLQKCFHEMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKK 240
            +L+KC HEMRPEVA+ LAKTVF VDER ILEKVDIPCTI+ TKTDV  PASVA+FMQK+
Sbjct: 181 SRLRKCLHEMRPEVAVSLAKTVFGVDERGILEKVDIPCTILHTKTDVAAPASVAIFMQKQ 240

Query: 241 IKGSCTVQVINTDGHFPHLTAHLELLQVLGKILGF 276
           IKGSCTV+VINT+GHFPHLTAHL+LLQVLG+ILGF
Sbjct: 241 IKGSCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272

BLAST of Tan0009812 vs. TAIR 10
Match: AT3G24420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 321.2 bits (822), Expect = 7.9e-88
Identity = 156/270 (57.78%), Postives = 204/270 (75.56%), Query Frame = 0

Query: 7   NGSSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDWSFSGSI 66
           N   S L++A+NAKIIGSG  +MVLAHGFG +QS+WDKI+P LSQ ++VLVFDW FSG+I
Sbjct: 4   NQKISGLASAMNAKIIGSGERSMVLAHGFGGDQSVWDKIIPVLSQSFKVLVFDWLFSGAI 63

Query: 67  KDPNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPDLFQTLI 126
           KD  L+DP KY+S   F++DLI L++E+     +F+GHSMSG+IGC A  +RPDLF  L+
Sbjct: 64  KDQTLYDPSKYNSLDVFSDDLIALMEELKFGPVVFVGHSMSGVIGCAASIKRPDLFTNLL 123

Query: 127 LLCSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSVRKLQKC 186
           L+ +SPRYIN+EDY+GGFE  D++ II +I SNYE WA +F S VVD  D LSV++ +K 
Sbjct: 124 LIAASPRYINSEDYKGGFESKDIDTIITSIGSNYEAWAVDFSSFVVDSRDSLSVQRFEKS 183

Query: 187 FHEMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKKIKGSCT 246
             +M+PE AL LAK VF  DER+IL +V +PC +IQ   DVVVP SVA FMQ+KIKG  T
Sbjct: 184 LKKMKPETALALAKIVFGSDEREILGQVSVPCHVIQPGNDVVVPVSVAYFMQEKIKGKST 243

Query: 247 VQVI-NTDGHFPHLTAHLELLQVLGKILGF 276
           V++I +  GHFP +T+HLELL V+ ++L F
Sbjct: 244 VEIIEDAIGHFPQMTSHLELLGVMRRLLEF 273

BLAST of Tan0009812 vs. TAIR 10
Match: AT3G03990.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 225.7 bits (574), Expect = 4.5e-59
Identity = 106/265 (40.00%), Postives = 173/265 (65.28%), Query Frame = 0

Query: 9   SSSSLSTAVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDWSFSGSIKD 68
           S  ++  A+N +++G+G   + LAHGFGT+QS W  ILP  +Q YRV+++D   +GS+ +
Sbjct: 2   SQHNILEALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSV-N 61

Query: 69  PNLFDPQKYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPDLFQTLILL 128
           P+ FD  +Y++   + +DL+ ++D +G+ +  ++GHS+S +IG +A  +RP+LF  LIL+
Sbjct: 62  PDYFDFNRYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILI 121

Query: 129 CSSPRYINTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVVDEADPLSVRKLQKCFH 188
             SPR++N EDY GGFE  ++E++ + +E+NYE W   F  L V    P +VR+  +   
Sbjct: 122 GFSPRFLNDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLF 181

Query: 189 EMRPEVALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKKIKGSCTVQ 248
            MRP+++L +++TVF  D R +L  V +P  +IQT  DV VPASVA +++  + G  TV+
Sbjct: 182 NMRPDISLFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVE 241

Query: 249 VINTDGHFPHLTAHLELLQVLGKIL 274
            + T+GH P L+A  +L Q L + L
Sbjct: 242 TLKTEGHLPQLSAPAQLAQFLRRAL 265

BLAST of Tan0009812 vs. TAIR 10
Match: AT4G37470.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 213.0 bits (541), Expect = 3.0e-55
Identity = 101/255 (39.61%), Postives = 161/255 (63.14%), Query Frame = 0

Query: 16  AVNAKIIGSGREAMVLAHGFGTNQSLWDKILPKLSQYYRVLVFDWSFSGSIKDPNLFDPQ 75
           A N K+IGSG   +VL HGFGT+QS+W  ++P L   YRV+++D +      +P+ FD  
Sbjct: 7   AHNVKVIGSGEATIVLGHGFGTDQSVWKHLVPHLVDDYRVVLYD-NMGAGTTNPDYFDFD 66

Query: 76  KYSSYHGFAEDLITLLDEIGLTSTIFIGHSMSGLIGCLAYTQRPDLFQTLILLCSSPRYI 135
           +YS+  G++ DLI +L+++ + S IF+GHS+S +IG LA   RPDLF  ++++ +SPRY+
Sbjct: 67  RYSNLEGYSFDLIAILEDLKIESCIFVGHSVSAMIGVLASLNRPDLFSKIVMISASPRYV 126

Query: 136 NTEDYEGGFEVSDVEQIIANIESNYENWATNFPSLVV-DEADPLSVRKLQKCFHEMRPEV 195
           N  DY+GGFE  D+ Q+   I SNY+ W   F  L V  + D ++V++  +    MRP++
Sbjct: 127 NDVDYQGGFEQEDLNQLFEAIRSNYKAWCLGFAPLAVGGDMDSIAVQEFSRTLFNMRPDI 186

Query: 196 ALPLAKTVFFVDERDILEKVDIPCTIIQTKTDVVVPASVALFMQKKIKGSCTVQVINTDG 255
           AL + +T+F  D R IL  V +PC I+Q+  D+ VP  V+ ++   +     V+VI +DG
Sbjct: 187 ALSVGQTIFQSDMRQILPFVTVPCHILQSVKDLAVPVVVSEYLHANLGCESVVEVIPSDG 246

Query: 256 HFPHLTAHLELLQVL 270
           H P L++   ++ V+
Sbjct: 247 HLPQLSSPDSVIPVI 260

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
J9U5U92.6e-5941.98Probable strigolactone esterase DAD2 OS=Petunia hybrida OX=4102 GN=DAD2 PE=1 SV=... [more]
Q10QA57.5e-5940.00Strigolactone esterase D14 OS=Oryza sativa subsp. japonica OX=39947 GN=D14 PE=1 ... [more]
Q9SQR36.3e-5840.00Strigolactone esterase D14 OS=Arabidopsis thaliana OX=3702 GN=D14 PE=1 SV=1[more]
A0A109QYD31.2e-5642.11Strigolactone esterase RMS3 OS=Pisum sativum OX=3888 GN=RMS3 PE=1 SV=1[more]
Q9SZU74.3e-5439.61Probable esterase KAI2 OS=Arabidopsis thaliana OX=3702 GN=KAI2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023002408.15.1e-12983.64probable strigolactone esterase DAD2 [Cucurbita maxima][more]
KAG6587947.15.1e-12982.91putative strigolactone esterase DAD2, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_023530785.18.6e-12982.91probable strigolactone esterase DAD2 [Cucurbita pepo subsp. pepo][more]
XP_022933803.12.5e-12882.55probable strigolactone esterase DAD2 [Cucurbita moschata][more]
XP_022144907.19.9e-12580.36probable strigolactone esterase DAD2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1KTG92.5e-12983.64probable strigolactone esterase DAD2 OS=Cucurbita maxima OX=3661 GN=LOC111496258... [more]
A0A6J1F5V41.2e-12882.55probable strigolactone esterase DAD2 OS=Cucurbita moschata OX=3662 GN=LOC1114411... [more]
A0A6J1CV134.8e-12580.36probable strigolactone esterase DAD2 OS=Momordica charantia OX=3673 GN=LOC111014... [more]
A0A6J1JFG12.4e-12480.36probable strigolactone esterase DAD2 OS=Cucurbita maxima OX=3661 GN=LOC111485258... [more]
A0A6J1H9Y69.0e-12480.00probable strigolactone esterase DAD2 OS=Cucurbita moschata OX=3662 GN=LOC1114618... [more]
Match NameE-valueIdentityDescription
AT3G24420.17.9e-8857.78alpha/beta-Hydrolases superfamily protein [more]
AT3G03990.14.5e-5940.00alpha/beta-Hydrolases superfamily protein [more]
AT4G37470.13.0e-5539.61alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 30..260
e-value: 3.2E-14
score: 54.1
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 9..273
e-value: 8.2E-57
score: 194.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 19..272
NoneNo IPR availablePANTHERPTHR43039ESTERASE-RELATEDcoord: 10..274
NoneNo IPR availablePANTHERPTHR43039:SF7ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 10..274

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0009812.1Tan0009812.1mRNA