Tan0009699 (gene) Snake gourd v1

Overview
NameTan0009699
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Description(R)-mandelonitrile lyase 1-like
LocationLG08: 9806136 .. 9810839 (-)
RNA-Seq ExpressionTan0009699
SyntenyTan0009699
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCACTCCACATAAAGATTTTGAAATCCATGGAGCACCATAGGGCTACCCTTCTCCTAGTCATTCTTATCTCTATCTTTCACTTTCAATTGAAAGTCTCCCATTCCTCCAACTCCAATGAAGGTAAAACCTCACTCTCTCTCAACATTATTTTATTATTTTTTTATTCACACTCTCTCGACATTATTTAGTATGGCTAGCTTTCTGTTACCATAATAAGGCTAACAATTTTTTACCGTTGGATGTAAGGAGAGAAGTACCTCTAATTTATATCCCGTTTTTGGGCATGAAAATTATTGAATCTACCTCCAACAATTAATATATATGGTTATTTCATCGAATAATTTATCGTTCTGTTAACTGTTATATGTAGTTTTAAAATTTGTTTACCTTATGAAAAGGATTCTAAAATTCTCTCTATTGATTATTATTGGCAAGGTTTAATTTTTTATTTTTTAATAGATTTTAGCTACATGAAGTTTGTACACGATGCCATTGAGTTTCCAGAAAAAGAAGAATATGACTACATAGTAATAGGAGGAGGAACCGCAGGTTGCCCATTAGCTGCAACATTATCATCAAAATACTCAGTCCTCCTTCTCGAAAGAGGGAGTGAACCCAAGAAATATCCATCTGTATTGAAAGAAGAAGGACTGTTGAATGTCTTTAGTGTTGAAGATGATGGCAAAAATCCCTTCCAACGGTTCACCTCTGAGGATGGTGTAGAGAACATAAGAGGACGAGTTCTTGGTGGCACTAGCATGCTCAATGGTGGTTTCTACTCAAGGGGTCATCGAGAGTTCTTTGAAACGGCAGGTGTTGATTGGGACATGGAATTGGTCGAGAAATCTTATCAATGGGCTGAAGAGACTGTGGTGTTTCGAGCAACTATGAGTCCTTGGCATTCAGCTTTCAAAAAAGGGTTGTTGGAAGCTGGGGTTGTACCTGATAATGGGTTTAATTTGAGACACCTTGTAGGGACTAAAATTGGAGGTTCCATATTTGATGACAAAGGTAACAGACATGGAGCTGTGGAACTTCTCAACAAGGCTGAACCAGAAAATATTAAAGTTGCAATTCGAGCCACTGTTGAAAGAATCCTCTTCTCTGGTGACTACTAATCAATCTCACACAATTTTATTCCATTGCCTCATTTTTGATAACTATTTGGTTTTTTATTTTTGGTCTTTAAAATTATGTTGGTTTTCTTTTATGTCTTTACCATAGATTTCATCTTTCTTTACGAGGAGACCTCTAAAATCTTAGTCAAATTCTAAAAGTAAAAAGATTTTTTTGAAACTACTTTCTTAGTTTTTAAAAGTTAGCTTGGTTTTTCAAAACATAGGTAAAAATTAAACCAATAGGTGGTATAAGTATTTAGAAATATTAGGTCCCGTTTGATAACCATTTGGTTTTTTGTTTTGGATTTTTTAAAATTATGCTGGTTGGTTCACAATTTCTCTTCATATTCTCTACAATGATTTCCATCTTTCTTGAAGAACCGTTTTAGTTTCTAGCTAAATTTCAAAAATAAAAATAATTTTTTTAGTTTTCAAAACTTAGCTTGATTTTTGAAAACATAGGTGGAAAGTAGATAATAAAATATAAAAACTCAGAATCAAATGTTTATTAAATGGGGTCTTTTTTTTTATATTTGAAAATTAAACTTATAAATACTACTTTCATTTATTAGTTTCTTTGTTTTGTGCCTATCTACCTTTATATGTTTTCAAAATGCAAGTGAAGTTTTAAAAACTAAAAAAATAGCTTTTGAAAATTTGGTTTTGTTTTAGGAATTCAATTTGAATGTTTTCTTAAAAAAGATGAAATTCATGAAAAATCGAGAGAATAGCTTAGTTTTCAAAAACAACCCATAAAAAAAATTAAATAGTTATCCAATGACCAAAATTTTCAAATTTTTACAAGCATTTTTTAAAAACTTGTGTAGGTTTATCTGCAAGTGGGGTTTCATATTCTGATTCGAAAGGAAAGTTACATACAGCATTCATCCGAAAGAAAGGAGAGATTATTTTGAGTGCTGGAGCCATTGGAAGTCCTCAACTTCTTCTTCTAAGTGGGATTGGCCCAAAATCTCATCTTTCATCCTTAAAACTACCGATCGTCCTTCACCAACCACATGTTGGTAAGTCCATGTCCGATAATCCTCGTTTCAGTAACCATATTGTTCTTCCATATACATTACCTATTACGGCTGTTAAAGTTGTCGGAACTTTAGAGAACAATATCCATTTTCAATCAATCACCAGCAGTTTACCATTTTCAATTCCGCCGTCGCATAGCCTTCTTCCTCCAAATTCTAATTCAGTCAACCTGGGCATAGCTACCATCGTTGGAAAATTCTCTGAAGTTCTTTCTGAGGGGTCGCTTCGGCTGAATTCCTCCACTGATGTGAAGAAGAGTCCAATTGTCCGATTCAATTATTATTCACATCCCGATGATCTTGCTAAATGTGTTAGAGGAGTAAGAAAAGTGGGCGATTTGCTTCAAACCCAAACGATGGTAAAGATTAAAACGCAAGATTTGGAGGGTAATAAAGTATTTCAGTTTCTGGGGCCTCCGTTGCCGGAAAATTTGTCGGATGATATTTCTGTTGAAAATTACTGTAAGAAAACAGTGACGACTTATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTTGTCGACGGTAATTACAGAGTCATCGGAATAAAAAACCTGCGTGTAGTTGACGGCTCCACTTTCCTCGATTCACCTGGAACCAATCCCATGGCTACTCTCCTGATGCTTGGCCGGTGAGTTATTTTATTTATGTGTACATGGAAAATATTTCAATGTTGATTATTTATTTATTTTTTATTATATTTGATTCTGTCCTCAATTTGTTTTCTTCTCTCACAGATTTGACATTTCTAATTGTTTTTTATTCTTGAGAGTTTTTCTGAAACTTCCATCTGTATTTCTTTTTTAAATGCTTGGAAAAAATGTTTGTTAGGTATTTGATAAGTATTTGGGCCGTTTGATAACGGTTTTTGTTTTTGGGTTTTGAAAAATTAAGCCTATAATACTCCTCTTATATCAATGAATTTCTATGTTTTCTTATCTAGTTTGTACTTATGTTTTCAAAAAGCTAAGGGTCTGTTTGGTATGCAATCCGAAAACAGAAATTTGAAAACAATGGATTCAATAAAAACAGAGTTGTATTTCATGTTTTCAGAAATGTGTTTGGTAGCAGATTTAGAAAATTGATTCCATTTTAACAATAGTTTAAAATGTGTTCTATAATATATTTATAGATCATAAAATTAGTTGTAGTTGCAAAACTAAATATTAGGTTGAATATAAGACATTATTAATTAATTTATGAACTTGTTTTTTAATATAAATTTTATATAATTATTATTTTATATTTATAATATATTATATTATACATATTTTAATTCAAAAAAAAGTAAATTAAGTTTATAAATATGAAATACTATATTTATTAAGATTTTTATCTTTGTTTAAAATAATTCTGCATTTTAAAATTTCAAATTCAGAATCTGGATACAATGAAAACATAAAAATGTTGTTTTTTAATTTCTACTATTTGGATCACAGAATCCAAAGCGATTTTTAAAAACATATCGCTAAACACGTATTCACCGAATTCGGTGAATCAAAACATAAAAGCAGAATCCGGACACGCACTCAAAGCAGGCCCTAAGTGTTTTAAAAACTAAAAAAAGTAGTTTTCAATTTTTATTTTTTTTTAAAATTTGGTGAAAAACTTAAATGACAAAAGATGGAAATCATTGTAGAGAAGATAATGAGAAATGTGGAAAAACAAACATAATTTTCAAAAACTAAAAACCATAAATCAAATGGTTATCAAACGAGATTTTGGTGTTTGGTTTTTTATTTTGAAAAATTCTACTAATCATTTTTCTCTATGGATTTCATATTTTTGAAGAAAACAATTCAATTCTTAATTAGGTAAATTCCAAAAGCAAAAACAAGGTTTTTGAAAATGACACTTTTTAGTTTTCAAAATTTGACTTATGTTTTGAAGACATATACAAAATGTAAATAGCCAAACCAATGAAATCTGTAGATGTAAGTAGTATTTATATAGGTTTAGTTTCCAAAAACAAAAAACACAACTATTCAAACAAGGTCTTAAAAATTAACAAAAGCATTAATACTTTCTTAAAAAATCACTAGAGCTGATGTACTAAAAAATTATTCAATGAGGCTGACACAATGTGGCTTGCAATATTTTACATCATTAAACATAAGAGAAAGAAATAAATTGTATAAGTATTCTGCTTTTGTATCCAATGAATATAGAAAACTAGCATATTTTTTTATTCTACATCCGACAGTTATTAAGAGACAATCACATTATGGCGGTCTCATTAAATAATTTTTTCTTGTACTAATTACACACGTGTGCTTGAGATTATTTTATTATCTTTTATTTCCAAATGTATACTAACAATTATAGAACTATTTTGCAGGTATGTTGGGCTTAAGATGTTGCAACAAAGAGTATAAACTACCGGAGGTGGTTTTGTAACATTATTGTTTTTCGGGTCTTGTATCGTATTTCTGATTTGTAAGGAATAAATGTGTTTGATAATTGTTCTTTATTTTTCTTTTCTTAAAAATGAACTAGGGTTACCAAGAAAATTCGAAAATTCGACCAAAGGAACATTGGTG

mRNA sequence

CCTCACTCCACATAAAGATTTTGAAATCCATGGAGCACCATAGGGCTACCCTTCTCCTAGTCATTCTTATCTCTATCTTTCACTTTCAATTGAAAGTCTCCCATTCCTCCAACTCCAATGAAGATTTTAGCTACATGAAGTTTGTACACGATGCCATTGAGTTTCCAGAAAAAGAAGAATATGACTACATAGTAATAGGAGGAGGAACCGCAGGTTGCCCATTAGCTGCAACATTATCATCAAAATACTCAGTCCTCCTTCTCGAAAGAGGGAGTGAACCCAAGAAATATCCATCTGTATTGAAAGAAGAAGGACTGTTGAATGTCTTTAGTGTTGAAGATGATGGCAAAAATCCCTTCCAACGGTTCACCTCTGAGGATGGTGTAGAGAACATAAGAGGACGAGTTCTTGGTGGCACTAGCATGCTCAATGGTGGTTTCTACTCAAGGGGTCATCGAGAGTTCTTTGAAACGGCAGGTGTTGATTGGGACATGGAATTGGTCGAGAAATCTTATCAATGGGCTGAAGAGACTGTGGTGTTTCGAGCAACTATGAGTCCTTGGCATTCAGCTTTCAAAAAAGGGTTGTTGGAAGCTGGGGTTGTACCTGATAATGGGTTTAATTTGAGACACCTTGTAGGGACTAAAATTGGAGGTTCCATATTTGATGACAAAGGTAACAGACATGGAGCTGTGGAACTTCTCAACAAGGCTGAACCAGAAAATATTAAAGTTGCAATTCGAGCCACTGTTGAAAGAATCCTCTTCTCTGGTTTATCTGCAAGTGGGGTTTCATATTCTGATTCGAAAGGAAAGTTACATACAGCATTCATCCGAAAGAAAGGAGAGATTATTTTGAGTGCTGGAGCCATTGGAAGTCCTCAACTTCTTCTTCTAAGTGGGATTGGCCCAAAATCTCATCTTTCATCCTTAAAACTACCGATCGTCCTTCACCAACCACATGTTGGTAAGTCCATGTCCGATAATCCTCGTTTCAGTAACCATATTGTTCTTCCATATACATTACCTATTACGGCTGTTAAAGTTGTCGGAACTTTAGAGAACAATATCCATTTTCAATCAATCACCAGCAGTTTACCATTTTCAATTCCGCCGTCGCATAGCCTTCTTCCTCCAAATTCTAATTCAGTCAACCTGGGCATAGCTACCATCGTTGGAAAATTCTCTGAAGTTCTTTCTGAGGGGTCGCTTCGGCTGAATTCCTCCACTGATGTGAAGAAGAGTCCAATTGTCCGATTCAATTATTATTCACATCCCGATGATCTTGCTAAATGTGTTAGAGGAGTAAGAAAAGTGGGCGATTTGCTTCAAACCCAAACGATGGTAAAGATTAAAACGCAAGATTTGGAGGGTAATAAAGTATTTCAGTTTCTGGGGCCTCCGTTGCCGGAAAATTTGTCGGATGATATTTCTGTTGAAAATTACTGTAAGAAAACAGTGACGACTTATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTTGTCGACGGTAATTACAGAGTCATCGGAATAAAAAACCTGCGTGTAGTTGACGGCTCCACTTTCCTCGATTCACCTGGAACCAATCCCATGGCTACTCTCCTGATGCTTGGCCGGTATGTTGGGCTTAAGATGTTGCAACAAAGAGTATAAACTACCGGAGGTGGTTTTGTAACATTATTGTTTTTCGGGTCTTGTATCGTATTTCTGATTTGTAAGGAATAAATGTGTTTGATAATTGTTCTTTATTTTTCTTTTCTTAAAAATGAACTAGGGTTACCAAGAAAATTCGAAAATTCGACCAAAGGAACATTGGTG

Coding sequence (CDS)

ATGGAGCACCATAGGGCTACCCTTCTCCTAGTCATTCTTATCTCTATCTTTCACTTTCAATTGAAAGTCTCCCATTCCTCCAACTCCAATGAAGATTTTAGCTACATGAAGTTTGTACACGATGCCATTGAGTTTCCAGAAAAAGAAGAATATGACTACATAGTAATAGGAGGAGGAACCGCAGGTTGCCCATTAGCTGCAACATTATCATCAAAATACTCAGTCCTCCTTCTCGAAAGAGGGAGTGAACCCAAGAAATATCCATCTGTATTGAAAGAAGAAGGACTGTTGAATGTCTTTAGTGTTGAAGATGATGGCAAAAATCCCTTCCAACGGTTCACCTCTGAGGATGGTGTAGAGAACATAAGAGGACGAGTTCTTGGTGGCACTAGCATGCTCAATGGTGGTTTCTACTCAAGGGGTCATCGAGAGTTCTTTGAAACGGCAGGTGTTGATTGGGACATGGAATTGGTCGAGAAATCTTATCAATGGGCTGAAGAGACTGTGGTGTTTCGAGCAACTATGAGTCCTTGGCATTCAGCTTTCAAAAAAGGGTTGTTGGAAGCTGGGGTTGTACCTGATAATGGGTTTAATTTGAGACACCTTGTAGGGACTAAAATTGGAGGTTCCATATTTGATGACAAAGGTAACAGACATGGAGCTGTGGAACTTCTCAACAAGGCTGAACCAGAAAATATTAAAGTTGCAATTCGAGCCACTGTTGAAAGAATCCTCTTCTCTGGTTTATCTGCAAGTGGGGTTTCATATTCTGATTCGAAAGGAAAGTTACATACAGCATTCATCCGAAAGAAAGGAGAGATTATTTTGAGTGCTGGAGCCATTGGAAGTCCTCAACTTCTTCTTCTAAGTGGGATTGGCCCAAAATCTCATCTTTCATCCTTAAAACTACCGATCGTCCTTCACCAACCACATGTTGGTAAGTCCATGTCCGATAATCCTCGTTTCAGTAACCATATTGTTCTTCCATATACATTACCTATTACGGCTGTTAAAGTTGTCGGAACTTTAGAGAACAATATCCATTTTCAATCAATCACCAGCAGTTTACCATTTTCAATTCCGCCGTCGCATAGCCTTCTTCCTCCAAATTCTAATTCAGTCAACCTGGGCATAGCTACCATCGTTGGAAAATTCTCTGAAGTTCTTTCTGAGGGGTCGCTTCGGCTGAATTCCTCCACTGATGTGAAGAAGAGTCCAATTGTCCGATTCAATTATTATTCACATCCCGATGATCTTGCTAAATGTGTTAGAGGAGTAAGAAAAGTGGGCGATTTGCTTCAAACCCAAACGATGGTAAAGATTAAAACGCAAGATTTGGAGGGTAATAAAGTATTTCAGTTTCTGGGGCCTCCGTTGCCGGAAAATTTGTCGGATGATATTTCTGTTGAAAATTACTGTAAGAAAACAGTGACGACTTATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTTGTCGACGGTAATTACAGAGTCATCGGAATAAAAAACCTGCGTGTAGTTGACGGCTCCACTTTCCTCGATTCACCTGGAACCAATCCCATGGCTACTCTCCTGATGCTTGGCCGGTATGTTGGGCTTAAGATGTTGCAACAAAGAGTATAA

Protein sequence

MEHHRATLLLVILISIFHFQLKVSHSSNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGGGTAGCPLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDGVENIRGRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATMSPWHSAFKKGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIRATVERILFSGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSSLPFSIPPSHSLLPPNSNSVNLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDDLAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKKTVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLKMLQQRV
Homology
BLAST of Tan0009699 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 2.1e-158
Identity = 279/541 (51.57%), Postives = 378/541 (69.87%), Query Frame = 0

Query: 6   ATLLLVILISIFHFQLKVSHS--SNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGGGTAGC 65
           + +LLV+ + +   Q    HS  + SN DFSY++F +DA +   +  YDY+++GGGT+GC
Sbjct: 7   SAILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGC 66

Query: 66  PLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDGVENIR 125
           PLAATLS KY VL+LERGS P  YP+VL  +G +     EDDGK P +RF SEDG++N+R
Sbjct: 67  PLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVR 126

Query: 126 GRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATMSPWHSAFK 185
           GRVLGGTSM+N G Y+R +   +  +GVDWDM+LV K+Y+W E+T+VF+    PW S   
Sbjct: 127 GRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTG 186

Query: 186 KGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIRATVER 245
              LEAGV P++GF+L H  GT+I GS FD+KG RH A ELLNK    N++V + A+VE+
Sbjct: 187 TAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEK 246

Query: 246 ILFS---GLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSS 305
           I+FS   GL+A+GV Y DS G  H AF+R KGE+I+SAG IG+PQLLLLSG+GP+S+LSS
Sbjct: 247 IIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSS 306

Query: 306 LKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSSLPFSI 365
           L +P+VL  P+VG+ + DNPR   +I+ P  +  T V V+G   +N  +Q   SSLPF+ 
Sbjct: 307 LNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLPFTT 366

Query: 366 PPSHSLLPPNSNSV-NLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDDL 425
           PP  S  P  S  + N   A    K +  LS GSL L SS++V+ SP V+FNYYS+P DL
Sbjct: 367 PP-FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDL 426

Query: 426 AKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKKTVTT 485
           + CV G++K+G+LL T  +   K +DL G + F  LG PLP++ +DD + E +C+++V +
Sbjct: 427 SHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVAS 486

Query: 486 YWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLKMLQQ 541
           YWHYHGGCLVGKV+DG++RV GI  LRVVDGSTF  +P ++P    LMLGRYVG+K+LQ+
Sbjct: 487 YWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQE 544

BLAST of Tan0009699 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 4.5e-156
Identity = 273/540 (50.56%), Postives = 374/540 (69.26%), Query Frame = 0

Query: 6   ATLLLVILISIFHFQLKVSHS--SNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGGGTAGC 65
           + +LLV+ I + H Q    HS  + S+ DFSY+ F +DA +   +  YDY+++GGGT+GC
Sbjct: 7   SAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTSGC 66

Query: 66  PLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDGVENIR 125
           PLAATLS KY VL+LERGS P  YP+VL  +G +     EDDGK P +RF SEDG++N+R
Sbjct: 67  PLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVR 126

Query: 126 GRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATMSPWHSAFK 185
           GRVLGGTS++N G Y+R +   +  +GVDWDM+LV ++Y+W E+T+V++     W S  K
Sbjct: 127 GRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQSVTK 186

Query: 186 KGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIRATVER 245
              LEAGV P++GF+L H  GT+I GS FD+KG RH A ELLNK    N++V + A+VE+
Sbjct: 187 TAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEK 246

Query: 246 ILFS---GLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSS 305
           I+FS   GL+A+GV Y DS G  H AF+R KGE+I+SAG IG+PQLLLLSG+GP+S+LSS
Sbjct: 247 IIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSS 306

Query: 306 LKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSSLPFSI 365
           L +P+VL  P+VG+ + DNPR   +I+ P  +  T V V+G   +N  +Q   SSLPF+ 
Sbjct: 307 LNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLPFTT 366

Query: 366 PPSHSLLPPNSNSVNLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDDLA 425
           PP       +    N   A    K +  LS GSL L SS++V+ SP V+FNYYS+  DL+
Sbjct: 367 PPFGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDLS 426

Query: 426 KCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKKTVTTY 485
            CV G++K+G+LL T  +   K +DL G + F  LG PLP++ +DD + E +C+++V +Y
Sbjct: 427 HCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESVASY 486

Query: 486 WHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLKMLQQR 541
           WHYHGGCLVGKV+DG++RV GI  LRVVDGSTF  +P ++P    LMLGRYVG+K+LQ+R
Sbjct: 487 WHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQER 544

BLAST of Tan0009699 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 7.1e-154
Identity = 270/542 (49.82%), Postives = 375/542 (69.19%), Query Frame = 0

Query: 6   ATLLLVILISIFHFQLK--VSHSSNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGGGTAGC 65
           + +LLV+ I + H Q     S ++ S+ DFSY+ FV+DA +   +  YDYI++GGGTAGC
Sbjct: 7   SAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAGC 66

Query: 66  PLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDGVENIR 125
           PLAATLS+ YSVL+LERGS P +YP++L  +G +     EDDGK P +RF SEDG++N+R
Sbjct: 67  PLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDNVR 126

Query: 126 GRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATMSPWHSAFK 185
           GRVLGGTSM+N G Y R +  FF   G++WDM+LV ++Y+W E+T+VF      W +   
Sbjct: 127 GRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIG 186

Query: 186 KGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIRATVER 245
              LEAG++P+NGF++ HL GT++ GS FD+ G RH + ELLNK +P N++VA++A VE+
Sbjct: 187 TAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAVEK 246

Query: 246 ILF----SGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLS 305
           I+F    SG++A GV Y+DS G  H AF+R +GE+ILSAG IGSPQLLLLSG+GP+S+L+
Sbjct: 247 IIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESYLT 306

Query: 306 SLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSSLPFS 365
           SL + +V   P+VG+ + DNPR   +I+ P  +  + V V+G   +   +Q   SSLPF 
Sbjct: 307 SLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSISSLPFD 366

Query: 366 IPPSHSLLPPNSNSV-NLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 425
            PP  S  P  S  + N   A IV K    LS G++ LNSS+DV+  P V+FNYYS+  D
Sbjct: 367 TPP-FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTD 426

Query: 426 LAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKKTVT 485
           L+ CV G++K+G++L T  +   K +DL G   F  LG PLPEN +DD + E +C+++V 
Sbjct: 427 LSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRESVA 486

Query: 486 TYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLKMLQ 541
           +YWHYHGGCLVGKV+D  +RV GI  LRVVDGSTF  +P ++P    LMLGRY+G+++LQ
Sbjct: 487 SYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGIQILQ 545

BLAST of Tan0009699 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 3.7e-150
Identity = 269/542 (49.63%), Postives = 370/542 (68.27%), Query Frame = 0

Query: 6   ATLLLVILISIFHFQLKVSHS--SNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGGGTAGC 65
           + +L V+ + + H Q    HS  + S  DFSY+KFV++A +   +  YDYIVIGGGT+GC
Sbjct: 7   SVILFVLHLLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIGGGTSGC 66

Query: 66  PLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDGVENIR 125
           PLAATLS KY VLLLERG+   +YP+ L  +G       +DDGK P +RF SEDG++N+R
Sbjct: 67  PLAATLSEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSEDGIDNVR 126

Query: 126 GRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATMSPWHSAFK 185
            R+LGGT+++N G Y+R +  F+   G++WD++LV K+Y+W E+ +V +     W S   
Sbjct: 127 ARILGGTTIINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQSWQSVIG 186

Query: 186 KGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIRATVER 245
           +G LEAG++PDNGF+L H  GT++ GS FD+ G RH A ELLNK +P N+ VA++A+VE+
Sbjct: 187 EGFLEAGILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAVQASVEK 246

Query: 246 ILF----SGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLS 305
           ILF    S LSA GV Y+DS G  H AF+R  GE+I+SAG IG+PQLLLLSG+GP+S+LS
Sbjct: 247 ILFSSNTSNLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVGPESYLS 306

Query: 306 SLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSSLPFS 365
           SL + +V   P+VG+ + +NPR   +   P  +  + V V+G   +  ++Q   SSLPFS
Sbjct: 307 SLNITVVQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSLSSLPFS 366

Query: 366 IPPSHSLLPPNSNSV-NLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 425
            PP  SL P  S  + N   A IV +    LS GS+ LNSS+DV+ +P ++FNYYS+  D
Sbjct: 367 TPP-FSLFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNYYSNSTD 426

Query: 426 LAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKKTVT 485
           LA CV G++K+GDLL+T+ +   K +D+ G   F +LG PLPEN +DD S E +C   V 
Sbjct: 427 LANCVSGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETFCLDNVA 486

Query: 486 TYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLKMLQ 541
           +YWHYHGG LVGKV+D ++RV+GIK LRVVD STF   P ++P    LMLGRYVGL++LQ
Sbjct: 487 SYWHYHGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGRYVGLQILQ 545

BLAST of Tan0009699 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 5.3e-149
Identity = 263/542 (48.52%), Postives = 365/542 (67.34%), Query Frame = 0

Query: 6   ATLLLVILISIFHFQLK--VSHSSNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGGGTAGC 65
           A L+L + + + H Q     S ++ S  DFSY++FV+DA +   +  YDYI++GGGTAGC
Sbjct: 8   AILVLALHLFVLHLQYSEVQSLANTSAHDFSYLEFVYDANDTELEGTYDYIIVGGGTAGC 67

Query: 66  PLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDGVENIR 125
           PLAATLS+ YSVL+LERG+ P +YP++L  +G +     EDDG+ P +RF S DG++N+R
Sbjct: 68  PLAATLSANYSVLVLERGTLPTEYPNLLTSDGFIYNLQQEDDGQTPVERFVSGDGIDNVR 127

Query: 126 GRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATMSPWHSAFK 185
           GRVLGGTSM+N G Y R +  FF   G++WDM+LV K+Y W E+T+VF+     W +   
Sbjct: 128 GRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNKTYDWVEDTIVFKPDFQFWQNLTG 187

Query: 186 KGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIRATVER 245
              LE G++PDNGF+L HL GT++ GS FD+ G RH + ELLNK +P N++VA+ A VE+
Sbjct: 188 TAFLEVGILPDNGFSLDHLEGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVHAAVEK 247

Query: 246 ILF----SGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLS 305
           I+F    SG++A GV Y+DS G  H AF+R  GE+ILSAG IGSPQLLLLSG+G +S+L+
Sbjct: 248 IIFSSDSSGVTAIGVIYTDSNGTTHQAFVRGDGEVILSAGPIGSPQLLLLSGVGLESYLT 307

Query: 306 SLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSSLPFS 365
           SL + +V   P+VG+ + DNPR   +I+ P  +  + V V+G   +   +Q   SSLPFS
Sbjct: 308 SLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSISSLPFS 367

Query: 366 IPPSHSLLPPNSNSVNLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDDL 425
             P      P     N   A IV K    LS G++ L S++DV+ +P V FNYYS+  DL
Sbjct: 368 TAPFGFFPNPTYPLPNTTFAHIVNKVPGPLSHGTVLLQSTSDVRVAPNVTFNYYSNTTDL 427

Query: 426 AKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKKTVTT 485
           A CV G++K+G+ L +  +   K +DL G + F  LG PLPEN +DD + E +C++ V +
Sbjct: 428 AHCVSGMKKIGEFLSSDALKPYKVEDLPGIEGFDILGIPLPENQTDDAAFETFCREAVAS 487

Query: 486 YWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLKMLQQ 542
           YWHYHGGCLVG+V+D ++RV GI  LRVVDGSTF  +P ++P    LMLGRY+G K+LQ+
Sbjct: 488 YWHYHGGCLVGEVLDDDFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGTKILQE 547

BLAST of Tan0009699 vs. NCBI nr
Match: XP_031736419.1 ((R)-mandelonitrile lyase 1-like [Cucumis sativus])

HSP 1 Score: 859.4 bits (2219), Expect = 1.7e-245
Identity = 427/545 (78.35%), Postives = 479/545 (87.89%), Query Frame = 0

Query: 1   MEHH--RATLLLVILISIFHFQLKVSHSSNS--NEDFSYMKFVHDAIEFPEKEEYDYIVI 60
           MEH    A+ LL ILIS+  FQL VS SSN+  NEDF YMKFVHDA + P KE+YDYI+I
Sbjct: 1   MEHSLIMASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIII 60

Query: 61  GGGTAGCPLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSE 120
           GGGTAGCPLAATLSS +SVLLLERGSEP KYPSVLKE+  LNV++VEDDG+NPFQRF SE
Sbjct: 61  GGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE 120

Query: 121 DGVENIRGRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATM- 180
           DGVEN+RGRVLGGTSMLNGGFYSRGH+EFFETAGV WDMELV+K+Y+W EE+VVF A++ 
Sbjct: 121 DGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLN 180

Query: 181 SPWHSAFKKGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKV 240
           + W  AF+ GLLEAGV P NGF L H +GTKIGGSIFD +GNRHG+VELLNKA+P N+KV
Sbjct: 181 NGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKV 240

Query: 241 AIRATVERILFSGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPK 300
            +RATVE+I+FSGLSASGV YSDSKG+LHTA IRKKGEIILSAGAIGSPQLLLLSG+GPK
Sbjct: 241 VVRATVEKIIFSGLSASGVLYSDSKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPK 300

Query: 301 SHLSSLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSS 360
           SHLSSLKLP+VLHQPHVG+SMSDNPRF+ +IVLPY L ITAVKVVGTL++NIH QSIT  
Sbjct: 301 SHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGF 360

Query: 361 LPFSIPPSHSLLPPNSNSVNLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSH 420
           LPFS+PPS SLLP   NSVNL +ATIVGKFSEV SEGSL+LNSSTDVKK+PIVRFNYYSH
Sbjct: 361 LPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSH 420

Query: 421 PDDLAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKK 480
           PDDLAKCVRGVRK GD L+T T+ KIK QD EG + F FL PP+PENLSDD  VE +CK+
Sbjct: 421 PDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKE 480

Query: 481 TVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLK 540
           TVTTYWHYHGGCLVGKVVDGNYRV+GIKNLRVVDGSTF DSPGTNPMATL+MLGRYVGL 
Sbjct: 481 TVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLN 540

BLAST of Tan0009699 vs. NCBI nr
Match: XP_031736342.1 ((R)-mandelonitrile lyase 1-like [Cucumis sativus] >KAE8651659.1 hypothetical protein Csa_021315 [Cucumis sativus])

HSP 1 Score: 858.2 bits (2216), Expect = 3.8e-245
Identity = 426/545 (78.17%), Postives = 479/545 (87.89%), Query Frame = 0

Query: 1   MEHH--RATLLLVILISIFHFQLKVSHSSNS--NEDFSYMKFVHDAIEFPEKEEYDYIVI 60
           MEH    A+ LL ILIS+  FQL VS SSN+  NEDF YMKFVHDA + P KE+YDYI+I
Sbjct: 1   MEHSLIMASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIII 60

Query: 61  GGGTAGCPLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSE 120
           GGGTAGCPLAATL+S +SVLLLERGSEP KYPSVLKE+  LNV++VEDDG+NPFQRF SE
Sbjct: 61  GGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE 120

Query: 121 DGVENIRGRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATM- 180
           DGVEN+RGRVLGGTSMLNGGFYSRGH+EFFETAGV WDMELV+K+Y+W EE+VVF A++ 
Sbjct: 121 DGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLN 180

Query: 181 SPWHSAFKKGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKV 240
           + W  AF+ GLLEAGV P NGF L H +GTKIGGSIFD +GNRHG+VELLNKA+P N+KV
Sbjct: 181 NGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKV 240

Query: 241 AIRATVERILFSGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPK 300
            +RATVE+I+FSGLSASGV YSDSKG+LHTA IRKKGEIILSAGAIGSPQLLLLSG+GPK
Sbjct: 241 VVRATVEKIIFSGLSASGVLYSDSKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPK 300

Query: 301 SHLSSLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSS 360
           SHLSSLKLP+VLHQPHVG+SMSDNPRF+ +IVLPY L ITAVKVVGTL++NIH QSIT  
Sbjct: 301 SHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGF 360

Query: 361 LPFSIPPSHSLLPPNSNSVNLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSH 420
           LPFS+PPS SLLP   NSVNL +ATIVGKFSEV SEGSL+LNSSTDVKK+PIVRFNYYSH
Sbjct: 361 LPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSH 420

Query: 421 PDDLAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKK 480
           PDDLAKCVRGVRK GD L+T T+ KIK QD EG + F FL PP+PENLSDD  VE +CK+
Sbjct: 421 PDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKE 480

Query: 481 TVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLK 540
           TVTTYWHYHGGCLVGKVVDGNYRV+GIKNLRVVDGSTF DSPGTNPMATL+MLGRYVGL 
Sbjct: 481 TVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLN 540

BLAST of Tan0009699 vs. NCBI nr
Match: XP_022153314.1 ((R)-mandelonitrile lyase 1-like [Momordica charantia])

HSP 1 Score: 855.5 bits (2209), Expect = 2.5e-244
Identity = 422/542 (77.86%), Postives = 473/542 (87.27%), Query Frame = 0

Query: 1   MEHH-RATLLLVILISIFHFQLKV-SHSSNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGG 60
           M+H+  A  LL+ ++SIFHFQL+V S  +  N+D SYMKFV DA + PEKEEYDYI++GG
Sbjct: 1   MKHYSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGG 60

Query: 61  GTAGCPLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDG 120
           GTAGCPLA TLSSK+SVLLLERGSEP K+PSVL EEGLLN F+ EDD KNPFQRF SEDG
Sbjct: 61  GTAGCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDG 120

Query: 121 VENIRGRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATMSPW 180
           VEN+RGRVLGG+SM+N GFYSRGHRE+FE AGV+WDMELVEK+YQW EETVV R ++S  
Sbjct: 121 VENLRGRVLGGSSMINAGFYSRGHREYFEKAGVEWDMELVEKAYQWVEETVVSRPSLSAL 180

Query: 181 HSAFKKGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIR 240
              F+  LLE+GV+PDNGF LRHLVGT+IGGSIFD KGNRHGAVELLNKA P N+KVAIR
Sbjct: 181 QPTFRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNRHGAVELLNKANPTNLKVAIR 240

Query: 241 ATVERILFSGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHL 300
           ATVERI+FSGLSASGVSYSDS+GKLHTAFIR KGEIILSAGAIGSPQLLLLSGIGPKS L
Sbjct: 241 ATVERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQL 300

Query: 301 SSLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSSLPF 360
           SSLKLP+VLHQP+VG+ MSDNPRF   IVLP+    T+ KVVGTLENNIH Q++ S LPF
Sbjct: 301 SSLKLPVVLHQPYVGEFMSDNPRFGPSIVLPFPFVPTSAKVVGTLENNIHLQTVASPLPF 360

Query: 361 SIPPSHSLLPPNSNSVNLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 420
           SIPPS SLLPP SNSVNL +    GKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD
Sbjct: 361 SIPPSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 420

Query: 421 LAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKKTVT 480
           LA+CV GVRK+GDL++T TM KIKT+D EG KVFQF+GP LPENLSDD +V  +C K++T
Sbjct: 421 LARCVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSLT 480

Query: 481 TYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLKMLQ 540
           TYWHYHGGCLVGKVVDG+YRVIGI NLRVVDGSTF+DSPGTNPMATL+MLGRYVGLK+LQ
Sbjct: 481 TYWHYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFIDSPGTNPMATLMMLGRYVGLKVLQ 540

BLAST of Tan0009699 vs. NCBI nr
Match: XP_022153315.1 ((R)-mandelonitrile lyase 1-like [Momordica charantia])

HSP 1 Score: 851.3 bits (2198), Expect = 4.6e-243
Identity = 421/542 (77.68%), Postives = 471/542 (86.90%), Query Frame = 0

Query: 1   MEHH-RATLLLVILISIFHFQLKV-SHSSNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGG 60
           M+H+  A  LL+ ++SIFHFQL+V S  +  N+D SYMKFV DA + PEKEEYDYI++GG
Sbjct: 1   MKHYSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGG 60

Query: 61  GTAGCPLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDG 120
           GTAGCPLA TLSSK+SVLLLERGSEP K+PSVL EEGLLN F+ EDD KNPFQRF SEDG
Sbjct: 61  GTAGCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDG 120

Query: 121 VENIRGRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATMSPW 180
           VEN+RGRVLGG+SM+N GFYSRGH+EFFE AGV+WDMELVEK+YQW EETVV R ++S  
Sbjct: 121 VENLRGRVLGGSSMINAGFYSRGHQEFFEKAGVEWDMELVEKAYQWVEETVVSRPSLSAL 180

Query: 181 HSAFKKGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIR 240
              F+  LLE+GV+PDNGF LRHLVGT+IGGSIFD KGN HGAVELLNKA P NIKVAIR
Sbjct: 181 QPTFRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNPHGAVELLNKANPTNIKVAIR 240

Query: 241 ATVERILFSGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHL 300
            TVERI+FSGLSASGVSYSDS+GKLHTAFIR KGEIILSAGAIGSPQLLLLSGIGPKS L
Sbjct: 241 TTVERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQL 300

Query: 301 SSLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSSLPF 360
           SSLKLP+VLHQP+VG+ MSDNPRF   IVLP+    T+ KVVGTLENNIH Q++ S LPF
Sbjct: 301 SSLKLPVVLHQPYVGEFMSDNPRFGPSIVLPFPXVPTSAKVVGTLENNIHLQTVASPLPF 360

Query: 361 SIPPSHSLLPPNSNSVNLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 420
           SIPPS SLLPP SNSVNL +    GKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD
Sbjct: 361 SIPPSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 420

Query: 421 LAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKKTVT 480
           LA+CV GVRK+GDL++T TM KIKT+D EG KVFQF+GP LPENLSDD +V  +C K++T
Sbjct: 421 LARCVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSLT 480

Query: 481 TYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLKMLQ 540
           TYWHYHGGCLVGKVVDG+YRVIGI NLRVVDGSTF+DSPGTNPMATL+MLGRYVGLK+LQ
Sbjct: 481 TYWHYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFIDSPGTNPMATLMMLGRYVGLKVLQ 540

BLAST of Tan0009699 vs. NCBI nr
Match: XP_038891172.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 849.4 bits (2193), Expect = 1.8e-242
Identity = 427/546 (78.21%), Postives = 480/546 (87.91%), Query Frame = 0

Query: 1   MEHHR--ATLLLVILIS-IFHFQLKVSHSSNS--NEDFSYMKFVHDAIEFPEKEEYDYIV 60
           MEH R  A  LL  L+S + HFQL VS SSN+  NED  YMKFVHDA E P+KEEYDYI+
Sbjct: 1   MEHRRPMAAFLLFFLVSMLLHFQLGVSLSSNTNPNEDVRYMKFVHDASELPKKEEYDYII 60

Query: 61  IGGGTAGCPLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTS 120
           IGGGTAGCPLAATLSS +SVLLLERGSEP KYP+VL E+   NVF++EDDGKNPFQRF S
Sbjct: 61  IGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPTVLSEQTFTNVFTIEDDGKNPFQRFVS 120

Query: 121 EDGVENIRGRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATM 180
           EDGVEN+RGRVLGGTSMLNGGFYSRGHREFFETAGV+WDM+LVEK+Y+W EE+VVF+A++
Sbjct: 121 EDGVENLRGRVLGGTSMLNGGFYSRGHREFFETAGVNWDMKLVEKAYEWVEESVVFQASL 180

Query: 181 -SPWHSAFKKGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIK 240
            + W  AF+KGLLEAGV PDNGF+LRH VGTKIGGSIFD +GNRHGAVELLNKAEP N+K
Sbjct: 181 NNGWQYAFRKGLLEAGVGPDNGFDLRHSVGTKIGGSIFDKEGNRHGAVELLNKAEPNNLK 240

Query: 241 VAIRATVERILFSGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGP 300
           VAI+ATV++ILFSGLSASGVSYSDSKGKLH AF+RKKGEIILSAGAIGSPQLLLLSG+GP
Sbjct: 241 VAIQATVQKILFSGLSASGVSYSDSKGKLHRAFVRKKGEIILSAGAIGSPQLLLLSGVGP 300

Query: 301 KSHLSSLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITS 360
           KSHLSSLK+P+VL+Q HVG+ MSDNPR++ +I+LP+ L  TAVKVVGTLENNIH QSITS
Sbjct: 301 KSHLSSLKVPVVLNQHHVGQFMSDNPRYTVNILLPFPLVTTAVKVVGTLENNIHLQSITS 360

Query: 361 SLPFSIPPSHSLLPPNSNSVNLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYS 420
            LPFS+P S SL+P   +SVNL +ATI GKFSEVLS+G L L +STDVKKSP VRFNYYS
Sbjct: 361 YLPFSVPLSFSLIPHGFDSVNLSLATIAGKFSEVLSDGYLHL-TSTDVKKSPTVRFNYYS 420

Query: 421 HPDDLAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCK 480
           HPDDLAKCVRGVRK+GD L+TQT+  IK QD EG K F+FLGP LP+NLSD  SVE YCK
Sbjct: 421 HPDDLAKCVRGVRKIGDFLKTQTIENIKIQDFEGRKGFEFLGPRLPQNLSDVSSVEEYCK 480

Query: 481 KTVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGL 540
           KTVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTF +SPGTNPMATLLMLGRYVGL
Sbjct: 481 KTVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSESPGTNPMATLLMLGRYVGL 540

BLAST of Tan0009699 vs. ExPASy TrEMBL
Match: A0A6J1DIQ0 ((R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020835 PE=3 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.2e-244
Identity = 422/542 (77.86%), Postives = 473/542 (87.27%), Query Frame = 0

Query: 1   MEHH-RATLLLVILISIFHFQLKV-SHSSNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGG 60
           M+H+  A  LL+ ++SIFHFQL+V S  +  N+D SYMKFV DA + PEKEEYDYI++GG
Sbjct: 1   MKHYSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGG 60

Query: 61  GTAGCPLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDG 120
           GTAGCPLA TLSSK+SVLLLERGSEP K+PSVL EEGLLN F+ EDD KNPFQRF SEDG
Sbjct: 61  GTAGCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDG 120

Query: 121 VENIRGRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATMSPW 180
           VEN+RGRVLGG+SM+N GFYSRGHRE+FE AGV+WDMELVEK+YQW EETVV R ++S  
Sbjct: 121 VENLRGRVLGGSSMINAGFYSRGHREYFEKAGVEWDMELVEKAYQWVEETVVSRPSLSAL 180

Query: 181 HSAFKKGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIR 240
              F+  LLE+GV+PDNGF LRHLVGT+IGGSIFD KGNRHGAVELLNKA P N+KVAIR
Sbjct: 181 QPTFRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNRHGAVELLNKANPTNLKVAIR 240

Query: 241 ATVERILFSGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHL 300
           ATVERI+FSGLSASGVSYSDS+GKLHTAFIR KGEIILSAGAIGSPQLLLLSGIGPKS L
Sbjct: 241 ATVERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQL 300

Query: 301 SSLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSSLPF 360
           SSLKLP+VLHQP+VG+ MSDNPRF   IVLP+    T+ KVVGTLENNIH Q++ S LPF
Sbjct: 301 SSLKLPVVLHQPYVGEFMSDNPRFGPSIVLPFPFVPTSAKVVGTLENNIHLQTVASPLPF 360

Query: 361 SIPPSHSLLPPNSNSVNLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 420
           SIPPS SLLPP SNSVNL +    GKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD
Sbjct: 361 SIPPSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 420

Query: 421 LAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKKTVT 480
           LA+CV GVRK+GDL++T TM KIKT+D EG KVFQF+GP LPENLSDD +V  +C K++T
Sbjct: 421 LARCVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSLT 480

Query: 481 TYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLKMLQ 540
           TYWHYHGGCLVGKVVDG+YRVIGI NLRVVDGSTF+DSPGTNPMATL+MLGRYVGLK+LQ
Sbjct: 481 TYWHYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFIDSPGTNPMATLMMLGRYVGLKVLQ 540

BLAST of Tan0009699 vs. ExPASy TrEMBL
Match: A0A6J1DGG8 ((R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020836 PE=3 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 2.2e-243
Identity = 421/542 (77.68%), Postives = 471/542 (86.90%), Query Frame = 0

Query: 1   MEHH-RATLLLVILISIFHFQLKV-SHSSNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGG 60
           M+H+  A  LL+ ++SIFHFQL+V S  +  N+D SYMKFV DA + PEKEEYDYI++GG
Sbjct: 1   MKHYSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGG 60

Query: 61  GTAGCPLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDG 120
           GTAGCPLA TLSSK+SVLLLERGSEP K+PSVL EEGLLN F+ EDD KNPFQRF SEDG
Sbjct: 61  GTAGCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDG 120

Query: 121 VENIRGRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATMSPW 180
           VEN+RGRVLGG+SM+N GFYSRGH+EFFE AGV+WDMELVEK+YQW EETVV R ++S  
Sbjct: 121 VENLRGRVLGGSSMINAGFYSRGHQEFFEKAGVEWDMELVEKAYQWVEETVVSRPSLSAL 180

Query: 181 HSAFKKGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIR 240
              F+  LLE+GV+PDNGF LRHLVGT+IGGSIFD KGN HGAVELLNKA P NIKVAIR
Sbjct: 181 QPTFRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNPHGAVELLNKANPTNIKVAIR 240

Query: 241 ATVERILFSGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHL 300
            TVERI+FSGLSASGVSYSDS+GKLHTAFIR KGEIILSAGAIGSPQLLLLSGIGPKS L
Sbjct: 241 TTVERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQL 300

Query: 301 SSLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSSLPF 360
           SSLKLP+VLHQP+VG+ MSDNPRF   IVLP+    T+ KVVGTLENNIH Q++ S LPF
Sbjct: 301 SSLKLPVVLHQPYVGEFMSDNPRFGPSIVLPFPXVPTSAKVVGTLENNIHLQTVASPLPF 360

Query: 361 SIPPSHSLLPPNSNSVNLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 420
           SIPPS SLLPP SNSVNL +    GKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD
Sbjct: 361 SIPPSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 420

Query: 421 LAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKKTVT 480
           LA+CV GVRK+GDL++T TM KIKT+D EG KVFQF+GP LPENLSDD +V  +C K++T
Sbjct: 421 LARCVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSLT 480

Query: 481 TYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLKMLQ 540
           TYWHYHGGCLVGKVVDG+YRVIGI NLRVVDGSTF+DSPGTNPMATL+MLGRYVGLK+LQ
Sbjct: 481 TYWHYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFIDSPGTNPMATLMMLGRYVGLKVLQ 540

BLAST of Tan0009699 vs. ExPASy TrEMBL
Match: A0A6J1DH55 ((R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020834 PE=3 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.9e-242
Identity = 416/542 (76.75%), Postives = 471/542 (86.90%), Query Frame = 0

Query: 1   MEHHRA-TLLLVILISIFHFQLKV-SHSSNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGG 60
           M+H+   T LL+ +ISIFHFQL V S  + SN+D SYMKFVHDA + PE  EYDYI++GG
Sbjct: 1   MKHNSMDTFLLLFIISIFHFQLGVLSSHTISNQDVSYMKFVHDAKDLPETGEYDYIIVGG 60

Query: 61  GTAGCPLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDG 120
           G AGCPLA TLSSK+S+L+LERGSEP K+PSVL E+G    F+ EDDGKNPFQRF SEDG
Sbjct: 61  GAAGCPLATTLSSKFSILILERGSEPNKFPSVLTEQGRSKAFTAEDDGKNPFQRFVSEDG 120

Query: 121 VENIRGRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATMSPW 180
           VEN+RGRVLGG +M+NGGFYSRGHREFF+TAGV+WDMELVEK+YQW EETVV R  +S W
Sbjct: 121 VENLRGRVLGGGTMINGGFYSRGHREFFDTAGVEWDMELVEKAYQWVEETVVSRPNLSAW 180

Query: 181 HSAFKKGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIR 240
            SAF+  LLE+GV+PDNGF+LRHLVGT+IGGS FD KGNRHGAVELLNKA P N+KV IR
Sbjct: 181 QSAFRSALLESGVLPDNGFDLRHLVGTRIGGSTFDGKGNRHGAVELLNKANPANLKVVIR 240

Query: 241 ATVERILFSGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHL 300
           ATVERI+FSGLSASGVSYSDS+GKLHTAFIRKKGEI LSAGAIGSPQLLLLSG+GPKS L
Sbjct: 241 ATVERIIFSGLSASGVSYSDSEGKLHTAFIRKKGEIFLSAGAIGSPQLLLLSGVGPKSDL 300

Query: 301 SSLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSSLPF 360
           SSLKLP+VLHQPHVG+ MSDNPRF  +IVLP+ +  T  KVVGTL+NNIHFQS   S  F
Sbjct: 301 SSLKLPVVLHQPHVGQFMSDNPRFVTNIVLPFPIVSTTGKVVGTLKNNIHFQSFAGSSLF 360

Query: 361 SIPPSHSLLPPNSNSVNLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 420
           S+PPS SLLPP SNSVN+ +AT+VGKFS+V S GSLRLNSSTDVKKSPIVRFNYYSHPDD
Sbjct: 361 SVPPSFSLLPPRSNSVNMSLATVVGKFSKVDSMGSLRLNSSTDVKKSPIVRFNYYSHPDD 420

Query: 421 LAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKKTVT 480
           LA+CV GVRK+GDL++T TM KIKT++ EG KVFQFLGPPLPENLSD  SV  +C KT+T
Sbjct: 421 LARCVVGVRKIGDLIKTPTMEKIKTRNFEGKKVFQFLGPPLPENLSDYSSVGEFCHKTLT 480

Query: 481 TYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLKMLQ 540
           TYWHYHGGCLVGKVVDG+YRVIGI NLRVVDGSTF +SPGTNPMATL+MLGRYVGLK++Q
Sbjct: 481 TYWHYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFTESPGTNPMATLMMLGRYVGLKVMQ 540

BLAST of Tan0009699 vs. ExPASy TrEMBL
Match: A0A1S3BS25 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493083 PE=3 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.7e-238
Identity = 417/535 (77.94%), Postives = 465/535 (86.92%), Query Frame = 0

Query: 1   MEHH--RATLLLVILISIFHFQLKVSHSSNS--NEDFSYMKFVHDAIEFPEKEEYDYIVI 60
           MEH    A+  L ILIS+ HF L    SSN+  NEDF YMKFVHDA + P +EEYDYI+I
Sbjct: 14  MEHSLIMASFPLFILISMLHFHLGFPLSSNTSPNEDFRYMKFVHDASDLPTEEEYDYIII 73

Query: 61  GGGTAGCPLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSE 120
           GGGTAGCPLAATLSS +SVLLLERGSEP KYPSVLKE+  LNV++VEDDG+NPFQRF SE
Sbjct: 74  GGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE 133

Query: 121 DGVENIRGRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATM- 180
           DGVEN+RGRVLGGTSMLNGGFYSRGHREFFETAGV WDMELV+K+Y+W EE VVF A++ 
Sbjct: 134 DGVENLRGRVLGGTSMLNGGFYSRGHREFFETAGVKWDMELVKKAYEWVEEGVVFEASLN 193

Query: 181 SPWHSAFKKGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKV 240
           + W  AF+ GLLEAGV P N F+L H +GTKIGGSIFD +GNRHG+VELLNKA P N+KV
Sbjct: 194 NGWQYAFRNGLLEAGVGPYNEFDLNHRLGTKIGGSIFDKEGNRHGSVELLNKAHPNNLKV 253

Query: 241 AIRATVERILFSGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPK 300
            +RATVE+I+FSGLSA GV YSDSKGKLHTA IR+KGEIILSAGAIGSPQLLLLSG+GPK
Sbjct: 254 VVRATVEKIIFSGLSARGVLYSDSKGKLHTASIRRKGEIILSAGAIGSPQLLLLSGVGPK 313

Query: 301 SHLSSLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSS 360
           SHLSSLKLPIVLHQPHVG+SMSDNPRF+ ++VLPY L ITAVKVVGTL++NIH QSIT  
Sbjct: 314 SHLSSLKLPIVLHQPHVGQSMSDNPRFTVNMVLPYPLVITAVKVVGTLDDNIHLQSITGF 373

Query: 361 LPFSIPPSHSLLPPNSNSVNLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSH 420
           LPFS+PPS SLLP   +SVNL +AT+VGKFSEVLSEGSL+LNSSTDVKK+PIVRFNYYSH
Sbjct: 374 LPFSLPPSFSLLPRGFDSVNLSLATVVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSH 433

Query: 421 PDDLAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKK 480
           PDDLAKCVRGVRK+GD L+T T+ KIK QD EG + F FL PPLPENLSD   VE YCKK
Sbjct: 434 PDDLAKCVRGVRKMGDFLKTATIEKIKIQDFEGKRSFAFLEPPLPENLSDIGVVEKYCKK 493

Query: 481 TVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGR 531
           TVTTYWHYHGGCLVGKVVDGNYRV+G+KNLRVVDGSTF DSPGTNPMATL+MLGR
Sbjct: 494 TVTTYWHYHGGCLVGKVVDGNYRVMGVKNLRVVDGSTFSDSPGTNPMATLMMLGR 548

BLAST of Tan0009699 vs. ExPASy TrEMBL
Match: A0A6J1EP46 ((R)-mandelonitrile lyase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436113 PE=3 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 2.0e-236
Identity = 403/540 (74.63%), Postives = 462/540 (85.56%), Query Frame = 0

Query: 1   MEHHRATLLLVILISIFHFQLKVSHSSNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGGGT 60
           ME   AT+L +ILIS F F   +S ++  ++D SYMKFVH+A + PEK+EYDYI+IGGG 
Sbjct: 1   MERSIATILFLILISTFQFG-AISSNTIPDQDVSYMKFVHNAGDLPEKQEYDYIIIGGGA 60

Query: 61  AGCPLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDGVE 120
           AGCPLAATLSSK+ VLLLERGSEP KYPSVL E+GLLN F  +DDG+NPFQRFTSEDGVE
Sbjct: 61  AGCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQGLLNAFLAQDDGQNPFQRFTSEDGVE 120

Query: 121 NIRGRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATMSPWHS 180
           NIRGR+LGG++M+N GFYSRGHR+FFETAGV+WDME+VE +YQW EETVV +  ++ W S
Sbjct: 121 NIRGRILGGSTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQWVEETVVSKPILNAWQS 180

Query: 181 AFKKGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIRAT 240
           AFK  LLEAGV PDNGFNL HL+GTK GGSIFD KGNRHGAVELLNKAEP N+KVA+ AT
Sbjct: 181 AFKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDGKGNRHGAVELLNKAEPMNLKVAVNAT 240

Query: 241 VERILFSGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSS 300
           V +ILF+GLSA+GVSYSDSKGK+HTAFIRKKGEI LSAGAIGSP LLL SG+GPKSHLSS
Sbjct: 241 VRQILFNGLSANGVSYSDSKGKIHTAFIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSS 300

Query: 301 LKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITSSLPFSI 360
           LKLP+V HQP+VG+ MSDNPRF   IVLP+ L  ++VKVVGTL++N+H Q+  S  PF  
Sbjct: 301 LKLPVVHHQPYVGEFMSDNPRFGATIVLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLA 360

Query: 361 PPSHSLLPPNSNSVNLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDDLA 420
           PP+ SLLPP + S+N  +   VGKFSEV SEG LRLNS+TD K + IVRFNYYSHPDDLA
Sbjct: 361 PPTFSLLPPQATSINPSLVIFVGKFSEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLA 420

Query: 421 KCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCKKTVTTY 480
           +CV GVRKVGDLL+TQTM KIKTQDLEGNK FQF+G PLPENL DD SVE YC+KTVTTY
Sbjct: 421 RCVEGVRKVGDLLKTQTMEKIKTQDLEGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTY 480

Query: 481 WHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLKMLQQR 540
           WHYHGGCLVGKVVDG+YRVIG+KNLRVVDGSTF DSPGTNPMATL+MLGRYVGLK+LQ+R
Sbjct: 481 WHYHGGCLVGKVVDGDYRVIGMKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQER 539

BLAST of Tan0009699 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 520.8 bits (1340), Expect = 1.3e-147
Identity = 262/547 (47.90%), Postives = 369/547 (67.46%), Query Frame = 0

Query: 7   TLLLVILISIFHFQLKVSHSSNS----NEDFSYMKFVHDAIEFPEKEEYDYIVIGGGTAG 66
           T L+V+L+      L V H SN+    N    +M+F+ +A +F  ++ YDYI++GGGTAG
Sbjct: 12  TALVVLLL------LGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAG 71

Query: 67  CPLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDGVENI 126
           CPLAATLS  + VLLLERG  P   P+V+  +G L   +  ++  +P Q F SE+GV N 
Sbjct: 72  CPLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNA 131

Query: 127 RGRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATMSPWHSAF 186
           RGRVLGG+S +N GFYSR  ++FFE +G+ WD+  V +SY+W E  +VFR  +  W +A 
Sbjct: 132 RGRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAI 191

Query: 187 KKGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIRATVE 246
           +  LLE GV P NGF L H VGTKIGGS FD  G RH + +LL  A   NI+VA+ ATVE
Sbjct: 192 RDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVE 251

Query: 247 RILF--------SGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGP 306
           R+L         S +SA GV Y D  G+ H A IR +GE+ILSAGA+GSPQLL LSGIGP
Sbjct: 252 RVLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGP 311

Query: 307 KSHLSSLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITS 366
           +S+LS+  +P+ L QPHVG  + DNPR    IV P  +  + ++VVG  E+    ++ ++
Sbjct: 312 RSYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASN 371

Query: 367 SLPFSIPPSHSLLPPNSNSVNLGIATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYS 426
            +PF+ P     +   ++ + + + TI+ K    +S G LRL +STDV+ +P+VRFNY+S
Sbjct: 372 VIPFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPVVRFNYFS 431

Query: 427 HPDDLAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDISVENYCK 486
            P DL +CV G RK+G++L+++ M     ++  GN+ F+F+G PLP + S+D+ + ++C+
Sbjct: 432 DPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCR 491

Query: 487 KTVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGL 542
           +TV+T WHYHGG +VGKVVD + +VIG+ +LR+VDGSTF  SPGTNP ATL+MLGRY+GL
Sbjct: 492 RTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGL 551

BLAST of Tan0009699 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 413.7 bits (1062), Expect = 2.3e-115
Identity = 238/554 (42.96%), Postives = 320/554 (57.76%), Query Frame = 0

Query: 26  SSNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGGGTAGCPLAATLSSKYSVLLLERGSEPK 85
           SS+   ++S+M+   DA   P    YDYI+IGGGTAGCPLAATLS   SVLLLERG  P 
Sbjct: 24  SSDKAPNYSFMR---DATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERGDSPY 83

Query: 86  KYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDGVENIRGRVLGGTSMLNGGFYSRGHREF 145
             P++ +        S +    +P QRF SEDGV N R RVLGG S LN GFY+R   ++
Sbjct: 84  NNPNITRLSAFGAALS-DLSESSPSQRFVSEDGVINARARVLGGGSALNAGFYTRAGTKY 143

Query: 146 FETAGVDWDMELVEKSYQWAEETVVFRATMSPWHSAFKKGLLEAGVVPDNGFNLRHLVGT 205
               G  WD  L  +SYQW E  V F+  M  W +A + GLLEAG+VP+NGF   H+ GT
Sbjct: 144 VRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDHINGT 203

Query: 206 KIGGSIFDDKGNRHGAVELLNKAEPENIKVAIRATVERILFSGLS-----ASGVSYSDSK 265
           K GG+IFD  GNRH A +LL  A+P+ I V + ATV RILF         A+GV Y D  
Sbjct: 204 KFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVVYRDRT 263

Query: 266 GKLHTAFIRK--KGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPIVLHQPHVGKSMSD 325
           G+ H A++++    EIILSAG +GSPQLL+LSG+GP + L +  + +V+ QPHVG+ M D
Sbjct: 264 GQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMYD 323

Query: 326 NPRFSNHIVLPYTLPITAVKVVGTLENNIHFQ-----------------SITSSLPFSIP 385
           NP  +  +  P  + ++ ++VVG      + +                 S T        
Sbjct: 324 NPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDYYAMFS 383

Query: 386 PSHSLLPPNSNSVNLGIATIVG-----KFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHP 445
           P  +LL  NS +         G     K    LS G L L  + + K +PIV FNY+ HP
Sbjct: 384 PRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLEL-KTRNPKDNPIVTFNYFQHP 443

Query: 446 DDLAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENL---------SDDI 505
           DDL +CVRG++ +  ++Q++   + K  D+    +   L    P NL         S   
Sbjct: 444 DDLKRCVRGIQTIERVVQSKAFSRYKYADVSFEYLLN-LTASTPVNLRPPRSGPGASLPP 503

Query: 506 SVENYCKKTVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLM 542
           S E +C+ TVTT WHYHGGC+VG+VVDG+Y+VIGI  LRV+D ST    PGTNP AT++M
Sbjct: 504 SAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTNPQATVMM 563

BLAST of Tan0009699 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 391.0 bits (1003), Expect = 1.6e-108
Identity = 230/540 (42.59%), Postives = 312/540 (57.78%), Query Frame = 0

Query: 47  EKEEYDYIVIGGGTAGCPLAATLSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSV---E 106
           +   YDYIVIGGGTAGCPLAATLS  +SVL+LERG  P    +V      L  F +   +
Sbjct: 59  QDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANV----SFLRNFHIGLAD 118

Query: 107 DDGKNPFQRFTSEDGVENIRGRVLGGTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQ 166
               +  Q F S DGV N R RVLGG S +N GFYSR    F + AG  WD +LV++SY 
Sbjct: 119 ISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYP 178

Query: 167 WAEETVVFRATMSPWHSAFKKGLLEAGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVE 226
           W E  +V +  ++ W  A +  LLE GV P NGF   H+ GTKIGG+IFD  G RH A E
Sbjct: 179 WVEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAE 238

Query: 227 LLNKAEPENIKVAIRATVERILF--SGL--SASGVSYSDSKGKLHTAFI--RKKGEIILS 286
           LL  A P+ ++V I ATV++I+F  SG     +GV + D KG  H A +  RK  E+ILS
Sbjct: 239 LLAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILS 298

Query: 287 AGAIGSPQLLLLSGIGPKSHLSSLKLPIVLHQPHVGKSMSDNPRFSNHIVLPYTLPI--T 346
           +GAIGSPQ+L+LSGIGPK  L  LK+P+VL   HVGK M+DNP   N I++P   PI  +
Sbjct: 299 SGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNP--MNTILVPSKAPIEQS 358

Query: 347 AVKVVGTLENNIHFQSIT--SSLPFSIPPSHSLLPPNSNSVNLGI--------------- 406
            ++ VG  +  ++ ++ T     P SI   + ++  N N +   I               
Sbjct: 359 LIQTVGITKMGVYVEASTGFGQSPESIHTHYGIM-SNKNELFSTIPAKQRRPEATQAYIT 418

Query: 407 ------------ATIVGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDDLAKCVRGV 466
                       + I+ K +  +S G L L  +T+V  +P V FNY+ HP DL +CV  +
Sbjct: 419 RNKYQLHEAFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVEAI 478

Query: 467 RKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPL-----PENLSDDISVENYCKKTVTTYW 526
           R V  ++ +   +     D +       L         P+ L+D  S+  +CK TV T W
Sbjct: 479 RLVSKVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIW 538

Query: 527 HYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSPGTNPMATLLMLGRYVGLKMLQQRV 542
           HYHGGCLVGKVV  N +V+G+  LRV+DGSTF +SPGTNP AT++M+GRY+G+K+L++R+
Sbjct: 539 HYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRERL 588

BLAST of Tan0009699 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 389.8 bits (1000), Expect = 3.5e-108
Identity = 234/563 (41.56%), Postives = 319/563 (56.66%), Query Frame = 0

Query: 23  VSHSSNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGGGTAGCPLAATLSSKYSVLLLERGS 82
           + H+S S +   Y  F+ DA   P    +DYI+IGGGT+GC LAATLS   SVL+LERG 
Sbjct: 18  IFHASCSPDKAGYYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGG 77

Query: 83  EPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDGVENIRGRVLGGTSMLNGGFYSRGH 142
            P   P+    E      S     K+  Q F SEDGV N R RVLGG S+LN GFY+R  
Sbjct: 78  APYDNPTATDIENFATTLS-NTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAG 137

Query: 143 REFFETAGVDWDMELVEKSYQWAEETVVFRATMSPWHSAFKKGLLEAGVVPDNGFNLRHL 202
            E+ +    +W  + VE +Y+W E+ V F+  +  W +AFK GLLEAG  P NGF   H+
Sbjct: 138 DEYVKE--TEWKTDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHI 197

Query: 203 VGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIRATVERILFSGL-----SASGVSYS 262
            GTKIGG+IFD  G+RH A +LL  A P NI V + A+V +ILF+        A GV + 
Sbjct: 198 YGTKIGGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQ 257

Query: 263 DSKGKLHTAFIRKK--GEIILSAGAIGSPQLLLLSGIGPKSHLSSLKL-PIVLHQPHVGK 322
           D+ G LH A + K    E+ILSAGAIGSPQLL+LSGIGP +HL++  + P+VL  P VG+
Sbjct: 258 DANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQ 317

Query: 323 SMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSI---------------------- 382
            M DNP  +  I  P  + ++ ++VVG  +   + +                        
Sbjct: 318 GMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLN 377

Query: 383 ---TSSLPFSIPPSHSL--LPPNSNSVN--LGIAT----IVGKFSEVLSEGSLRLNSSTD 442
              TS    +  P+ S   +    N +N  L   T    I+ K +  +S G L L  +T+
Sbjct: 378 EMQTSRTTSTTSPTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELR-NTN 437

Query: 443 VKKSPIVRFNYYSHPDDLAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQ-FLGPPL- 502
              +P VRFNYY  P+DL  CV G+  +  ++ ++   K K  D   + +    L  P  
Sbjct: 438 PDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLMLSVPTN 497

Query: 503 --PENLSDDISVENYCKKTVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFLDSP 541
             P +++   ++  +C  TV T WHYHGGC VG+VVD NYRV+GI +LRV+DGSTFL SP
Sbjct: 498 LRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSP 557

BLAST of Tan0009699 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 387.9 bits (995), Expect = 1.3e-107
Identity = 229/557 (41.11%), Postives = 323/557 (57.99%), Query Frame = 0

Query: 9   LLVILISIFHFQLKVSHSSNSNEDFSYMKFVHDAIEFPEKEEYDYIVIGGGTAGCPLAAT 68
           L+++ ISIF F L    ++          F+ DA   P+   +DYI+IGGGTAGC LAAT
Sbjct: 41  LILVYISIFFFFLVKLETT----------FMKDATLAPKNASFDYIIIGGGTAGCALAAT 100

Query: 69  LSSKYSVLLLERGSEPKKYPSVLKEEGLLNVFSVEDDGKNPFQRFTSEDGVENIRGRVLG 128
           LS   SVL+LERG  P + P+       +N   + +   +  Q F SEDGV N R RVLG
Sbjct: 101 LSQNASVLVLERGGSPYENPTATDMGNSVNTL-LNNTPNSWSQLFISEDGVYNTRPRVLG 160

Query: 129 GTSMLNGGFYSRGHREFFETAGVDWDMELVEKSYQWAEETVVFRATMSPWHSAFKKGLLE 188
           G S++NGGFYSR   ++ E A  +W+ME VE +Y+W E+ +VF   +  W  AFK GLLE
Sbjct: 161 GGSVINGGFYSRAGNDYVEEA--EWEMEEVEAAYEWVEKKLVFEPQVIEWQKAFKDGLLE 220

Query: 189 AGVVPDNGFNLRHLVGTKIGGSIFDDKGNRHGAVELLNKAEPENIKVAIRATVERILFSG 248
           AG  PDNGF   H+ GTKIGG+IFD  G+RH A  LL  A P  I V + A+V ++LF+ 
Sbjct: 221 AGESPDNGFTYDHIYGTKIGGTIFDRAGHRHTAANLLEYANPNRIVVYLHASVHKVLFT- 280

Query: 249 LSASGVSYSDSKGKLHTAFIRKK--GEIILSAGAIGSPQLLLLSGIGPKSHLSSLKL-PI 308
             A  V + D+ G  H A +  K   E+ILSAGA+GSPQLL+LSG+GP  HL +  + P+
Sbjct: 281 TEAYEVLFEDANGVFHKANLANKATNEVILSAGALGSPQLLMLSGVGPAVHLEAHGVNPL 340

Query: 309 VLHQPHVGKSMSDNPRFSNHIVLPYTLPITAVKVVGTLENNIHFQSITS-SLPFSIPPSH 368
           VL QP VG+ M+DNP     I  P  + ++ ++ VG  + + + + ++  SL F I    
Sbjct: 341 VLDQPMVGQGMADNPMNFVAIPSPQPVELSLIQAVGITKFDSYIEGLSGLSLSFDITRRF 400

Query: 369 -----SLLPPNSNSVNLGIAT----------------------IVGKFSEVLSEGSLRLN 428
                +LL   S++ +  I T                      I  K     S+G ++L 
Sbjct: 401 FDGVLNLLNETSHTTSRKILTQSIAVLLKSFDVKLEVRMNGGLIFQKVDGPASKGHMKLR 460

Query: 429 SSTDVKKSPIVRFNYYSHPDDLAKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQ-FLG 488
            +T+ + +P V FNYY  P+DL KCV+G+  +  ++ ++   K K   +   ++    L 
Sbjct: 461 -NTNPRDNPSVTFNYYQEPEDLNKCVKGLNTIIRMINSKAFSKYKYPGVTARELLNLMLA 520

Query: 489 PPL---PENLSDDISVENYCKKTVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTF 531
            P+   P +++   +++ +C  TVT+ WHYHGGC VGKVVD NY+V+GI  LRV+DGSTF
Sbjct: 521 LPINLRPRHVTSAFNLKQFCIDTVTSVWHYHGGCQVGKVVDKNYKVLGIDGLRVIDGSTF 580

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P527062.1e-15851.57(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q945K24.5e-15650.56(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
P527077.1e-15449.82(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
O242433.7e-15049.63(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1[more]
O500485.3e-14948.52(R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_031736419.11.7e-24578.35(R)-mandelonitrile lyase 1-like [Cucumis sativus][more]
XP_031736342.13.8e-24578.17(R)-mandelonitrile lyase 1-like [Cucumis sativus] >KAE8651659.1 hypothetical pro... [more]
XP_022153314.12.5e-24477.86(R)-mandelonitrile lyase 1-like [Momordica charantia][more]
XP_022153315.14.6e-24377.68(R)-mandelonitrile lyase 1-like [Momordica charantia][more]
XP_038891172.11.8e-24278.21(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1DIQ01.2e-24477.86(R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020835 P... [more]
A0A6J1DGG82.2e-24377.68(R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020836 P... [more]
A0A6J1DH551.9e-24276.75(R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020834 P... [more]
A0A1S3BS251.7e-23877.94(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493083 PE=3 SV=... [more]
A0A6J1EP462.0e-23674.63(R)-mandelonitrile lyase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT1G73050.11.3e-14747.90Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.12.3e-11542.96Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.11.6e-10842.59Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.13.5e-10841.56Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51930.11.3e-10741.11Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 36..346
e-value: 3.2E-55
score: 185.9
coord: 341..540
e-value: 1.9E-30
score: 104.1
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 390..530
e-value: 8.3E-29
score: 100.9
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 51..320
e-value: 4.6E-32
score: 111.6
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 279..293
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 124..147
NoneNo IPR availableGENE3D3.30.410.40coord: 171..479
e-value: 3.8E-169
score: 565.7
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 7..540
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 7..540
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 371..486
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 51..529
e-value: 3.8E-169
score: 565.7
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 34..539

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0009699.1Tan0009699.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors