Tan0009632 (gene) Snake gourd v1

Overview
NameTan0009632
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionmetal tolerance protein 4
LocationLG09: 60809303 .. 60813902 (+)
RNA-Seq ExpressionTan0009632
SyntenyTan0009632
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCAATTTGCAGTTAAAAAGGCGTATCTCTTTCTCTGCCTTTCTGAGCGCTTCTCTGAGATCAGCTGCATTGGTTTTGCAGCTTCTGTTTCTCGATTGGCAGAGAGGAGGAAGGCGATTATCCCGGTGCCGGTGCTGGTGCTCGCCGGATAAAATGGACGGAGATTCGGATTCGAGTCCCAAGGCGGCGCTGTTGGGAGGAGCGATGAAGCGCGGCGGCAGCGGGAGACGCGGCCGACTCAGTCGCCGTTACTCGGTGAACTCGCTCCGGAGCGAGTTTATTTCCAGATTGCCTGAAAAAGTGAGGTCTCGTCTTCAGGACGTCGAATCTCCCTATGAAATTGATCTTTCGCAGTCGACTGGCTTCAGCAGAGGTTCTTCTCTTTCTCTTACTGTTTATCGATCCTTATCTCTTTCGCTTTTTTTCTGATCAATTTCTGTTTGGTTGATGAGAGAATGAAGATGATAGACGAGGAGAATTCTTGTTGTATTTTATGGTTATTTTGTTTTTTTCGCTTGTTCTTTGGTGTAAATGTGAGATGATGGCGAAGACGGAGGCAAAGCTAGACTCTGTTTTCTTTCCTCTGTATACGATAGCGAAATCTGATTTAATCAGAAATGGTTGCGATGAGGAATGTTAATTTCCAATGCGCGTTTTTTTTTTATTTCCATTAAGGTTGCCTTCTTCTGAGTAGTCTCCATCTTTAGTGTTCTATGGGAGGGAGAACAGATACTACTGACTTATTGGTTTATTATAGGGGAAAAGGATTACTATGAAACGCAACTCGCTACCTTAAAATCATTTGAGGAAGTTGATTCATTAGTGGCATCTGACTGCATTGATGAGGAAGACAGTGAAGAACAAGCTCAGCAAGAGAGGGCCATGAAGATCTCAAATTATGCGAATATAGTACTTCTTTTGTTGAAGGTCGTCTATGTTACCGTGAATTTATTACTCTAGATTAAGAATTCAATTCCGTAGGCTTATAATGAGCAGCATGAATTATGATAACACAACAATTTTCAGCAAACGACTTTGTAGTCCTAAATCAACTATTTATTAGGAAAAGGAAATGAGTTAGAAAGGGAATTGTTAAAATTAGTATGGTGACTACGGTCTCAATGCTGAGACTGCTAATTGCCCCAATTAACATTGAGCATATATAAAGTCCCTCTTGAAGAGCTGCTTATTAAACAGATAAATGTCAAGGTTGTTTCATGGTTTATTAAAGGCTCTGTTCTGGTCCCTTATTCCAAACCTTGAATTTACTCTCTAGTGACAGTTTGCAGATCTATGCTACAGTGCGGAGTGGATCCATAGCCATTGCTGCATCAACACTGGATTCCTTACTTGATCTCATGGCTGGTGGAATACTTTGGTTTACTCACTTGTACATGAAGCGAGTAAATATTTATAATTACCCGATTGGAAAGCTGAGGGTCCAGCCAGTAGGCATTATTGTATTTGCTGCTGTCATGGCTACACTAGGTATCTGATTTTATCTAAAACCAGTGTGAGAGTGCATTGATTTTCTCATTCAGTCATTTGAACCATGATCGGAGAGTAGGAAGGTCTAGTAATTTTATTGCCTATTTACCTCATATTCCTGCTAAAAATGAAGGTCCCTTTCTCTATTATAACAAAATAAAGTCTTGACATCCACTATTGATGGATATTACACCAATGTCTTGCAACTAGCAAAGACCCAAGTCTGTGCAATTATCTATTCATTTTGTATTCTAGAATCTTTAAGGGTTGAATTATCTTGGTTCTGTCAAATTTTCATTTGACCTTTATTGTTGGCCAAGTATGATTTGAGTTCCTTTAACAATGTGGAGTAGCTAAACCATTTCAATTTCTTGTTCAGGCTTTCAGGTATTGCTTCAAGCTGTAGAACAACTAATTCAAGATAAACCGTCTGAAGGTCTGTCATCAGAACAATTCATCTGGTTGTGTGCAATTATGATCACTGCCACAGTAGTAAAACTTGCCCTCTGGTTGTACTGCAAAAGCTCAAGAAATGATATTGTGCGCGCTTACGCAAAGGTTGTTAAACCTACAATTATTACTTTGTAATTGTTTCCAAAGAAAATAATTCTTCTATATTATGGCATAATCCTTCCGATGCTAATTCCCAGGATCATTACTTCGATGTGGTAACAAATGTGGTTGGATTAGTTGCAGCTATCCTTGGTGATAAGATCTTTTGGTGGATAGATCCGGTCGGTGCTATTGCCCTAGCCATATATACAATTTTAAATTGGTCTGGAACTGTATGGGAAAATGCAGGTACAATTTTTTACTCGCATCTTGTTCCCTTTCTCTTAAAATGAAAATTTCTAAACAACTTGCATGTTGAAAGGATGTATGTTGATATGCTTCTTTTTGGGAAATCCCATGATTACTTCTTTTACTCTCGCGTGATATAACAAAATACTAAAACAACTAGATCTGGAAAGGAGTTTTATAATAATAGGTCTAGCACCCAGGGTGCAATCATTCTTTTGTTGTCGCAGTAAATGTGATGAACTTTCAAAAAGATTTGAAATAACCATATTTTTGTCATATGCCTGAAGTAATTGTATGTTTGTAACATTTGCAGTTTTGGTTTCTTCCTTTTGAATTGTTTGCCATGCTCCCATTTCATATTACTTTGTTTTCCTTCCAGTTCACAACTAACACGAGGTAATTCTTGTTGTAGTTTCACTCGTGGGGAAATCAGCCCCTCCTGAAGTCCTGCAAAAGTTGACATATCTTGTCATTAGGCATCCTGAGGTCAAACGCGTTGACACAGTTCGTGCTTACACCTTCGGCGTTCTTTATTTTGTAGAGGTCAGTCATACCCTATAAACCTTTCGTCAGTTACTTGTCTATCTTGCATTAAAAAAAAATTCTATGTCTCGTTGGCACCGTGATCAGATCACCGACCCTGTAGGAACATTTTCATTCTTTAATTTTCTATTTATTGAGAAATTATTCTCTTGGACTGGTACAATGTGGTTCACAGTTTGTATTCCATAGTTATTAAGGGACATGCAAATTTTTAAATCAACATTCAATGGTTATTAAAAGGTAGCCACATTATGACGGTTTCGTGGACTAATTGAATCCTATTATTATCTTTTTAATCTCACACTGCAGCATAAGTTCATAGCTCTAATAACTTGTCATCCTTTGCATAAGTTCATAGCTCTAATAGCTTGTCATCCTTTCCATAGTTCAATGTTTAAGTTATCTATCAGAGGAATGTTGAAATTCCCACCTCCACAATTGCACTAAGTCTTTATTGTAAAAATTAAGAGGAAAAAAAATCTAAATGACTAATAACTTCTAAGGTACTTCCTTCAGCAGAGCTCCTAAAAGATGCATACAATGTTGCTTGATGCATAATGTTGAAACCATCCTGTTCTGATTTCTGAAAGTTTCCCAAATTTGCACGCATTAGAAAAATGTTAAGACAAATCTCAAACCAATAAATATACTGAGATGCCACTGATACCATTGATTGCTACTTCCATTCTGCAAGTTAAGACTTAACAGATACATATCGTTTCAAACACTTGTCTAATATAGAAGTATACTTGTTCTGACAATCAATCAGGTGGACATTGAACTTCCGGAGGAGCTGCCTTTGAAAGAAGCACACGCCATCGGAGAGACGTTGCAGATAAAGATTGAAAAGCTTCCAGAAGTTGAGAGGGCTTTTGTTCATCTTGACTTTGAATGTGATCATAAACCAGAACACTCTATCCTCAGCAGGCTACCAAACAGTCAGCCCTGATGTTTTCTTGGTATCTGTTCCCTAACCATTTTGACTTGTTATGTTATGTCTCATGTCTAGCATGTGTGCATCTTTTTTGTTAGAAAAATAAAGCTATTTATTTTCAATCAGATCAAATAATCAGTTTTTACTTTCAATTTTATTTTTGTCTCTTCTTTAGGTATGCTCCCCCCAGGTAGGTTATTTTGGGGGGGTGCTTTTGGCCTTCCTACCCAAAATTATTACGTTCTGAAGTAATCTAAAAGTGCTTTTATAAAAAGCTAAAAGCACTTGAGGTGCATCCTAAGAAAGCACGAGCAATATTGTTTGTTATGGAATACTTTTTACTAAAGTGTTTTTATATATACATGTGTCAAGTTTAGGAGAAATTTTGTAGCCAACGATAGTTGGAAGGAAAGGAGTTTTCTTAAATTTATAAAAGTTTCTAATACATCTACCGATAATGTTTTTCATTCCAAAAGATAAGGGGTTGTTCGGGATGCTGAGGGGATTATAAAAACATGGATTATAAAAGTATGTGAAATTATACAATATTATTTAAAACGTAGAGTTGTTTAGTCTGGGATTATAATAGTTCGTATTTGGGGTGCATGGTATTCCACATGTTATAATAATCTGTGCTCCGAACATGCCCTAAATGTTTATTATGGAAATTTAACCAATGTATTGTACTTTTAACTCATTTTTGTGTTCGGTTGAAGATTTACTGTACAATTGCTCCACTTTTAACATTCAAATTATGAGGTTGTATATATGCAGAGTGAGAAAATGTGGAGGTTAGTTCAATGTTAGGAACGTTGTATTTATAAGGAGTTGGAG

mRNA sequence

TTCAATTTGCAGTTAAAAAGGCGTATCTCTTTCTCTGCCTTTCTGAGCGCTTCTCTGAGATCAGCTGCATTGGTTTTGCAGCTTCTGTTTCTCGATTGGCAGAGAGGAGGAAGGCGATTATCCCGGTGCCGGTGCTGGTGCTCGCCGGATAAAATGGACGGAGATTCGGATTCGAGTCCCAAGGCGGCGCTGTTGGGAGGAGCGATGAAGCGCGGCGGCAGCGGGAGACGCGGCCGACTCAGTCGCCGTTACTCGGTGAACTCGCTCCGGAGCGAGTTTATTTCCAGATTGCCTGAAAAAGTGAGGTCTCGTCTTCAGGACGTCGAATCTCCCTATGAAATTGATCTTTCGCAGTCGACTGGCTTCAGCAGAGGGGAAAAGGATTACTATGAAACGCAACTCGCTACCTTAAAATCATTTGAGGAAGTTGATTCATTAGTGGCATCTGACTGCATTGATGAGGAAGACAGTGAAGAACAAGCTCAGCAAGAGAGGGCCATGAAGATCTCAAATTATGCGAATATAGTACTTCTTTTGTTGAAGATCTATGCTACAGTGCGGAGTGGATCCATAGCCATTGCTGCATCAACACTGGATTCCTTACTTGATCTCATGGCTGGTGGAATACTTTGGTTTACTCACTTGTACATGAAGCGAGTAAATATTTATAATTACCCGATTGGAAAGCTGAGGGTCCAGCCAGTAGGCATTATTGTATTTGCTGCTGTCATGGCTACACTAGGCTTTCAGGTATTGCTTCAAGCTGTAGAACAACTAATTCAAGATAAACCGTCTGAAGGTCTGTCATCAGAACAATTCATCTGGTTGTGTGCAATTATGATCACTGCCACAGTAGTAAAACTTGCCCTCTGGTTGTACTGCAAAAGCTCAAGAAATGATATTGTGCGCGCTTACGCAAAGCTATCCTTGGTGATAAGATCTTTTGGTGGATAGATCCGGTCGGTGCTATTGCCCTAGCCATATATACAATTTTAAATTGGTCTGGAACTGTATGGGAAAATGCAGTTTCACTCGTGGGGAAATCAGCCCCTCCTGAAGTCCTGCAAAAGTTGACATATCTTGTCATTAGGCATCCTGAGGTCAAACGCGTTGACACAGTTCGTGCTTACACCTTCGGCGTTCTTTATTTTGTAGAGGTGGACATTGAACTTCCGGAGGAGCTGCCTTTGAAAGAAGCACACGCCATCGGAGAGACGTTGCAGATAAAGATTGAAAAGCTTCCAGAAGTTGAGAGGGCTTTTGTTCATCTTGACTTTGAATGTGATCATAAACCAGAACACTCTATCCTCAGCAGGCTACCAAACAGTCAGCCCTGATGTTTTCTTGGTATCTGTTCCCTAACCATTTTGACTTGTTATGTTATGTCTCATGTCTAGCATGTGTGCATCTTTTTTGTTAGAAAAATAAAGCTATTTATTTTCAATCAGATCAAATAATCAGTTTTTACTTTCAATTTTATTTTTGTCTCTTCTTTAGGTATGCTCCCCCCAGGTAGGTTATTTTGGGGGGGTGCTTTTGGCCTTCCTACCCAAAATTATTACGTTCTGAAGTAATCTAAAAGTGCTTTTATAAAAAGCTAAAAGCACTTGAGGTGCATCCTAAGAAAGCACGAGCAATATTGTTTGTTATGGAATACTTTTTACTAAAGTGTTTTTATATATACATGTGTCAAGTTTAGGAGAAATTTTGTAGCCAACGATAGTTGGAAGGAAAGGAGTTTTCTTAAATTTATAAAAGTTTCTAATACATCTACCGATAATGTTTTTCATTCCAAAAGATAAGGGGTTGTTCGGGATGCTGAGGGGATTATAAAAACATGGATTATAAAAGTATGTGAAATTATACAATATTATTTAAAACGTAGAGTTGTTTAGTCTGGGATTATAATAGTTCGTATTTGGGGTGCATGGTATTCCACATGTTATAATAATCTGTGCTCCGAACATGCCCTAAATGTTTATTATGGAAATTTAACCAATGTATTGTACTTTTAACTCATTTTTGTGTTCGGTTGAAGATTTACTGTACAATTGCTCCACTTTTAACATTCAAATTATGAGGTTGTATATATGCAGAGTGAGAAAATGTGGAGGTTAGTTCAATGTTAGGAACGTTGTATTTATAAGGAGTTGGAG

Coding sequence (CDS)

ATGGACGGAGATTCGGATTCGAGTCCCAAGGCGGCGCTGTTGGGAGGAGCGATGAAGCGCGGCGGCAGCGGGAGACGCGGCCGACTCAGTCGCCGTTACTCGGTGAACTCGCTCCGGAGCGAGTTTATTTCCAGATTGCCTGAAAAAGTGAGGTCTCGTCTTCAGGACGTCGAATCTCCCTATGAAATTGATCTTTCGCAGTCGACTGGCTTCAGCAGAGGGGAAAAGGATTACTATGAAACGCAACTCGCTACCTTAAAATCATTTGAGGAAGTTGATTCATTAGTGGCATCTGACTGCATTGATGAGGAAGACAGTGAAGAACAAGCTCAGCAAGAGAGGGCCATGAAGATCTCAAATTATGCGAATATAGTACTTCTTTTGTTGAAGATCTATGCTACAGTGCGGAGTGGATCCATAGCCATTGCTGCATCAACACTGGATTCCTTACTTGATCTCATGGCTGGTGGAATACTTTGGTTTACTCACTTGTACATGAAGCGAGTAAATATTTATAATTACCCGATTGGAAAGCTGAGGGTCCAGCCAGTAGGCATTATTGTATTTGCTGCTGTCATGGCTACACTAGGCTTTCAGGTATTGCTTCAAGCTGTAGAACAACTAATTCAAGATAAACCGTCTGAAGGTCTGTCATCAGAACAATTCATCTGGTTGTGTGCAATTATGATCACTGCCACAGTAGTAAAACTTGCCCTCTGGTTGTACTGCAAAAGCTCAAGAAATGATATTGTGCGCGCTTACGCAAAGCTATCCTTGGTGATAAGATCTTTTGGTGGATAG

Protein sequence

MDGDSDSSPKAALLGGAMKRGGSGRRGRLSRRYSVNSLRSEFISRLPEKVRSRLQDVESPYEIDLSQSTGFSRGEKDYYETQLATLKSFEEVDSLVASDCIDEEDSEEQAQQERAMKISNYANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLRVQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALWLYCKSSRNDIVRAYAKLSLVIRSFGG
Homology
BLAST of Tan0009632 vs. ExPASy Swiss-Prot
Match: Q10PP8 (Metal tolerance protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP4 PE=2 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 3.8e-76
Identity = 155/227 (68.28%), Postives = 182/227 (80.18%), Query Frame = 0

Query: 31  RRYSVNSLRSEFISRLPEKVRSRLQDVESPYEIDLSQSTGFSRGEKDYYETQLATLKSFE 90
           RR SV S+R EF+SRLP+KV   + D E P  +D S+S G   GEK+YYE Q ATL+SFE
Sbjct: 18  RRNSVGSMRGEFVSRLPKKVLDAV-DPERPSHVDFSRSKGLREGEKEYYEKQFATLRSFE 77

Query: 91  EVDSLVASDCIDEEDS-EEQAQQERAMKISNYANIVLLLLKIYATVRSGSIAIAASTLDS 150
           EVDS+  S+ + EED   EQ Q E AMKISNYAN++LL LKIYAT++SGSIAIAASTLDS
Sbjct: 78  EVDSIEESNVMSEEDDIAEQKQSEFAMKISNYANMILLALKIYATIKSGSIAIAASTLDS 137

Query: 151 LLDLMAGGILWFTHLYMKRVNIYNYPIGKLRVQPVGIIVFAAVMATLGFQVLLQAVEQLI 210
           LLDLMAGGILWFTHL MK +N+Y YPIGKLRVQPVGII+FAAVMATLGFQV +QAVE+LI
Sbjct: 138 LLDLMAGGILWFTHLSMKSINVYKYPIGKLRVQPVGIIIFAAVMATLGFQVFVQAVEKLI 197

Query: 211 QDKPSEGLSSEQFIWLCAIMITATVVKLALWLYCKSSRNDIVRAYAK 257
            ++  + L+  Q  WL +IMI ATVVKLALWLYC++S N IVRAYAK
Sbjct: 198 VNETPDKLTPVQLTWLYSIMIFATVVKLALWLYCRTSGNKIVRAYAK 243

BLAST of Tan0009632 vs. ExPASy Swiss-Prot
Match: Q6Z7K5 (Metal tolerance protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP3 PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.1e-75
Identity = 162/262 (61.83%), Postives = 201/262 (76.72%), Query Frame = 0

Query: 1   MDGDSDSSPKAALLGGAMKRGGSGRRGRLSRRYSVNSLRSEFISRLPEKVRSRLQDVESP 60
           MDGD   +P   LLGG    GGS R   L RR S  SLRS F+SRLP+KVR         
Sbjct: 1   MDGDDRRTP---LLGG---EGGSTRPPSLRRRDSARSLRSTFLSRLPDKVRGGGDPERPA 60

Query: 61  YEIDLSQSTGFSRGEKDYYETQLATLKSFEEVDSL-----VASDC-IDEEDSEEQAQQER 120
            ++DL+++ G S+GEK+YYE QLATLK FEEV++L       SD  + E + +EQ Q E 
Sbjct: 61  ADVDLTRAKGLSQGEKEYYEKQLATLKIFEEVEALCMPGEFESDAEVLELEDKEQKQSES 120

Query: 121 AMKISNYANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNY 180
           AMKISNYANI+LL+ K+YAT+++GS+AIAASTLDSLLD +AGGIL+FTHL MK VNIY Y
Sbjct: 121 AMKISNYANIILLVFKVYATIKTGSMAIAASTLDSLLDFLAGGILYFTHLTMKSVNIYKY 180

Query: 181 PIGKLRVQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATV 240
           PIGKLRVQPVGIIVFAA+MATLGFQVL+QA+EQL+++K  E ++ EQ IWL +IM++ATV
Sbjct: 181 PIGKLRVQPVGIIVFAAIMATLGFQVLIQAIEQLVENKAGEKMTPEQLIWLYSIMLSATV 240

Query: 241 VKLALWLYCKSSRNDIVRAYAK 257
           VKLAL++YC+SS N IV+AYAK
Sbjct: 241 VKLALYIYCRSSGNSIVQAYAK 256

BLAST of Tan0009632 vs. ExPASy Swiss-Prot
Match: Q9M2P2 (Putative metal tolerance protein C3 OS=Arabidopsis thaliana OX=3702 GN=MTPC3 PE=3 SV=2)

HSP 1 Score: 284.3 bits (726), Expect = 1.5e-75
Identity = 153/229 (66.81%), Postives = 185/229 (80.79%), Query Frame = 0

Query: 35  VNSLRSEFISRLPEKVRSRLQDVESPYEIDLSQSTGFSRGEKDYYETQLATLKSFEEVDS 94
           V+S++S F + LP+K+RS++ D E+P  +D+S++ G    EK+YYE QLATLKSFEEV+S
Sbjct: 30  VSSMKSNFFADLPQKLRSKI-DPENPLHLDVSKAAGLKEDEKEYYERQLATLKSFEEVES 89

Query: 95  LVA-------SDCIDEEDSEEQAQQERAMKISNYANIVLLLLKIYATVRSGSIAIAASTL 154
            +A        +  +EED  E+A QE AM+ISN+ANI LL LKIYATV+SGSIAIAASTL
Sbjct: 90  FLARSDEYTIDEKEEEEDRAERAAQELAMQISNWANIFLLALKIYATVKSGSIAIAASTL 149

Query: 155 DSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLRVQPVGIIVFAAVMATLGFQVLLQAVEQ 214
           DSLLDLMAGGILWFTHL MK VNIY YPIGKLRVQPVGII+FAAVMATLGFQVLL A EQ
Sbjct: 150 DSLLDLMAGGILWFTHLSMKNVNIYKYPIGKLRVQPVGIIIFAAVMATLGFQVLLVAAEQ 209

Query: 215 LIQDKPSEGLSSEQFIWLCAIMITATVVKLALWLYCKSSRNDIVRAYAK 257
           LI ++PSE ++  Q IWL +IM++AT +KL LW+YCKSSRN IVRAYAK
Sbjct: 210 LISNEPSEKMNHVQLIWLYSIMLSATAIKLVLWIYCKSSRNHIVRAYAK 257

BLAST of Tan0009632 vs. ExPASy Swiss-Prot
Match: Q5NA18 (Metal tolerance protein 5 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP5 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 5.2e-41
Identity = 110/258 (42.64%), Postives = 159/258 (61.63%), Query Frame = 0

Query: 7   SSPKAALLGGAMKRGGSGRRGRLSRRYSVNSLRSEFISRLPEKVRSRLQDVESPYEIDLS 66
           S+ +A   GG    GG+G     S R + +  R       P +V+   Q+   P  +   
Sbjct: 19  SAVEAGSFGGGGDGGGAGAAAEKSWRLNFDGFR-------PPEVQ---QERRPPRGLHHH 78

Query: 67  QSTGFSRGEKD----YYETQLATLKSFEEVDSLVASDCI---DEEDSEEQAQQER-AMKI 126
                S+G +D    YY+ Q+  L+ F E+D+L     +    +E+ E+ A+ E  A+++
Sbjct: 79  CLGVLSQGPEDVVAEYYQQQVEMLEGFNEMDTLTDRGFLPGMSKEEREKVARSETLAIRL 138

Query: 127 SNYANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGK 186
           SN AN+VL   K+YA+VRSGS+AI ASTLDSLLDL++G ILWFT   M+  N Y YPIGK
Sbjct: 139 SNIANMVLFAAKVYASVRSGSLAIIASTLDSLLDLLSGFILWFTAFSMQTPNPYRYPIGK 198

Query: 187 LRVQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLA 246
            R+QP+GI+VFA+VMATLG Q++L++V  L+ D     L+ EQ  W+  IM+  T+VKLA
Sbjct: 199 KRMQPLGILVFASVMATLGLQIILESVRSLLSDGDEFSLTKEQEKWVVDIMLAVTLVKLA 258

Query: 247 LWLYCKSSRNDIVRAYAK 257
           L LYC++  N+IV+AYA+
Sbjct: 259 LVLYCRTFTNEIVKAYAQ 266

BLAST of Tan0009632 vs. ExPASy Swiss-Prot
Match: O80632 (Metal tolerance protein 11 OS=Arabidopsis thaliana OX=3702 GN=MTP11 PE=2 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.3e-39
Identity = 89/184 (48.37%), Postives = 125/184 (67.93%), Query Frame = 0

Query: 77  DYYETQLATLKSFEEVDSLVASDCIDEEDSEEQ----AQQERAMKISNYANIVLLLLKIY 136
           DYY+ Q+  L+ F E+D L     +     EEQ      +  A++ISN AN++L   K+Y
Sbjct: 63  DYYQQQVEMLEGFTEMDELAERGFVPGMSKEEQDNLAKSETLAIRISNIANMLLFAAKVY 122

Query: 137 ATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLRVQPVGIIVFAAV 196
           A+V SGS+AI ASTLDSLLDL++G ILWFT   M+  N Y YPIGK R+QP+GI+VFA+V
Sbjct: 123 ASVTSGSLAIIASTLDSLLDLLSGFILWFTAFSMQTPNPYQYPIGKKRMQPLGILVFASV 182

Query: 197 MATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALWLYCKSSRNDIVR 256
           MATLG Q++L+++  ++       L+ EQ  W+  IM++ T+VKL L LYC+S  N+IV+
Sbjct: 183 MATLGLQIILESLRTMLSSHKEFNLTKEQESWVVGIMLSVTLVKLLLVLYCRSFTNEIVK 242

BLAST of Tan0009632 vs. NCBI nr
Match: XP_023516649.1 (metal tolerance protein 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 442.2 bits (1136), Expect = 3.2e-120
Identity = 233/256 (91.02%), Postives = 243/256 (94.92%), Query Frame = 0

Query: 1   MDGDSDSSPKAALLGGAMKRGGSGRRGRLSRRYSVNSLRSEFISRLPEKVRSRLQDVESP 60
           MDG+ DS+PK ALL GA+KR GSGRRGRLSR YSVNSLRSEFISRLPEKV+S LQDVESP
Sbjct: 1   MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTGFSRGEKDYYETQLATLKSFEEVDSLVASDCIDEEDSEEQAQQERAMKISN 120
           YEIDLSQST FSRGEKDYYE Q+ATLKSFEEVDSLV SDCIDEEDSEE+AQ+ERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDL+AGGILWFTHLYMKRVNIY +PIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALW 240
           VQPVGIIVFAAVMATLGFQVLLQA EQLIQDKPSEGLS EQFIWLCAIMI+ATVVKLALW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLALW 240

Query: 241 LYCKSSRNDIVRAYAK 257
            YCKSSRNDIVRAYAK
Sbjct: 241 FYCKSSRNDIVRAYAK 256

BLAST of Tan0009632 vs. NCBI nr
Match: XP_038878436.1 (metal tolerance protein 4 [Benincasa hispida])

HSP 1 Score: 441.4 bits (1134), Expect = 5.4e-120
Identity = 234/256 (91.41%), Postives = 245/256 (95.70%), Query Frame = 0

Query: 1   MDGDSDSSPKAALLGGAMKRGGSGRRGRLSRRYSVNSLRSEFISRLPEKVRSRLQDVESP 60
           MDG SDSSPKAALLGG++  GGSGRRGRLSRRYSVNSLRSEFISRLPEK+RSR+QD ESP
Sbjct: 1   MDGSSDSSPKAALLGGSV-NGGSGRRGRLSRRYSVNSLRSEFISRLPEKLRSRIQDAESP 60

Query: 61  YEIDLSQSTGFSRGEKDYYETQLATLKSFEEVDSLVASDCIDEEDSEEQAQQERAMKISN 120
           Y+IDLSQS+GFSRGEKDYYE QLATLKSFE+VDSLV SDCIDEED EE+AQQERAMKISN
Sbjct: 61  YDIDLSQSSGFSRGEKDYYERQLATLKSFEDVDSLVTSDCIDEEDMEERAQQERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLR 180
           YANIVLLLLKIYAT+RSGSIAIAASTLDSLLDLMAGGILWFTHLYMK+VNIY YPIGKLR
Sbjct: 121 YANIVLLLLKIYATIRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKQVNIYKYPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALW 240
           VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSE LSSEQFIWLCAIM  ATVVKLALW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSESLSSEQFIWLCAIMTFATVVKLALW 240

Query: 241 LYCKSSRNDIVRAYAK 257
           LYCK+SRNDIVRAYAK
Sbjct: 241 LYCKNSRNDIVRAYAK 255

BLAST of Tan0009632 vs. NCBI nr
Match: XP_022988141.1 (metal tolerance protein 4 [Cucurbita maxima])

HSP 1 Score: 441.0 bits (1133), Expect = 7.1e-120
Identity = 233/256 (91.02%), Postives = 242/256 (94.53%), Query Frame = 0

Query: 1   MDGDSDSSPKAALLGGAMKRGGSGRRGRLSRRYSVNSLRSEFISRLPEKVRSRLQDVESP 60
           MDG  DS+PK ALL GA+KR GSGRRGRLSR YSVNSLRSEFISRLPEKV+S LQDVESP
Sbjct: 1   MDGGPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTGFSRGEKDYYETQLATLKSFEEVDSLVASDCIDEEDSEEQAQQERAMKISN 120
           YEIDLSQST FSRGEKDYYE Q+ATLKSFEEVDSLV SDCIDEEDSEE+AQ+ERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDL+AGGILWFTHLYMKRVNIY +PIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALW 240
           VQPVGIIVFAAVMATLGFQVLLQA EQLIQDKPSEGLS EQFIWLCAIMI+ATVVKLALW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLALW 240

Query: 241 LYCKSSRNDIVRAYAK 257
            YCKSSRNDIVRAYAK
Sbjct: 241 FYCKSSRNDIVRAYAK 256

BLAST of Tan0009632 vs. NCBI nr
Match: KAG6590238.1 (Metal tolerance protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 441.0 bits (1133), Expect = 7.1e-120
Identity = 232/256 (90.62%), Postives = 243/256 (94.92%), Query Frame = 0

Query: 1   MDGDSDSSPKAALLGGAMKRGGSGRRGRLSRRYSVNSLRSEFISRLPEKVRSRLQDVESP 60
           MDG+ D++PK ALL GA+KR GSGRRGRLSR YSVNSLRSEFISRLPEKV+S LQDVESP
Sbjct: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTGFSRGEKDYYETQLATLKSFEEVDSLVASDCIDEEDSEEQAQQERAMKISN 120
           YEIDLSQST FSRGEKDYYE Q+ATLKSFEEVDSLV SDCIDEEDSEE+AQ+ERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDL+AGGILWFTHLYMKRVNIY +PIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALW 240
           VQPVGIIVFAAVMATLGFQVLLQA EQLIQDKPSEGLS EQFIWLCAIMI+ATVVKL LW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW 240

Query: 241 LYCKSSRNDIVRAYAK 257
           LYCKSSRNDIVRAYAK
Sbjct: 241 LYCKSSRNDIVRAYAK 256

BLAST of Tan0009632 vs. NCBI nr
Match: XP_008451104.1 (PREDICTED: metal tolerance protein 4 isoform X1 [Cucumis melo])

HSP 1 Score: 440.3 bits (1131), Expect = 1.2e-119
Identity = 234/256 (91.41%), Postives = 245/256 (95.70%), Query Frame = 0

Query: 1   MDGDSDSSPKAALLGGAMKRGGSGRRGRLSRRYSVNSLRSEFISRLPEKVRSRLQDVESP 60
           MDGDSD SPKA LLGG++  GGSGRRGRLSRRYSVNSLRSEFISRLP+K+RS LQDVESP
Sbjct: 1   MDGDSDLSPKAPLLGGSV-NGGSGRRGRLSRRYSVNSLRSEFISRLPDKLRSHLQDVESP 60

Query: 61  YEIDLSQSTGFSRGEKDYYETQLATLKSFEEVDSLVASDCIDEEDSEEQAQQERAMKISN 120
           YEIDLS+STGFSRGEKDYYE QLATLKSFE+VDSLV+SDCIDEED EE+AQQERAMKISN
Sbjct: 61  YEIDLSKSTGFSRGEKDYYERQLATLKSFEDVDSLVSSDCIDEEDMEERAQQERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIY +PIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALW 240
           VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSE LSSEQF+WLCAIM  ATVVKLALW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSESLSSEQFVWLCAIMTFATVVKLALW 240

Query: 241 LYCKSSRNDIVRAYAK 257
           LYCK+SRNDIVRAYAK
Sbjct: 241 LYCKNSRNDIVRAYAK 255

BLAST of Tan0009632 vs. ExPASy TrEMBL
Match: A0A6J1JGE1 (metal tolerance protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111485472 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 3.4e-120
Identity = 233/256 (91.02%), Postives = 242/256 (94.53%), Query Frame = 0

Query: 1   MDGDSDSSPKAALLGGAMKRGGSGRRGRLSRRYSVNSLRSEFISRLPEKVRSRLQDVESP 60
           MDG  DS+PK ALL GA+KR GSGRRGRLSR YSVNSLRSEFISRLPEKV+S LQDVESP
Sbjct: 1   MDGGPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTGFSRGEKDYYETQLATLKSFEEVDSLVASDCIDEEDSEEQAQQERAMKISN 120
           YEIDLSQST FSRGEKDYYE Q+ATLKSFEEVDSLV SDCIDEEDSEE+AQ+ERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDL+AGGILWFTHLYMKRVNIY +PIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALW 240
           VQPVGIIVFAAVMATLGFQVLLQA EQLIQDKPSEGLS EQFIWLCAIMI+ATVVKLALW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLALW 240

Query: 241 LYCKSSRNDIVRAYAK 257
            YCKSSRNDIVRAYAK
Sbjct: 241 FYCKSSRNDIVRAYAK 256

BLAST of Tan0009632 vs. ExPASy TrEMBL
Match: A0A5A7THE5 (Metal tolerance protein 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold824G00130 PE=4 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 5.9e-120
Identity = 234/256 (91.41%), Postives = 245/256 (95.70%), Query Frame = 0

Query: 1   MDGDSDSSPKAALLGGAMKRGGSGRRGRLSRRYSVNSLRSEFISRLPEKVRSRLQDVESP 60
           MDGDSD SPKA LLGG++  GGSGRRGRLSRRYSVNSLRSEFISRLP+K+RS LQDVESP
Sbjct: 1   MDGDSDLSPKAPLLGGSV-NGGSGRRGRLSRRYSVNSLRSEFISRLPDKLRSHLQDVESP 60

Query: 61  YEIDLSQSTGFSRGEKDYYETQLATLKSFEEVDSLVASDCIDEEDSEEQAQQERAMKISN 120
           YEIDLS+STGFSRGEKDYYE QLATLKSFE+VDSLV+SDCIDEED EE+AQQERAMKISN
Sbjct: 61  YEIDLSKSTGFSRGEKDYYERQLATLKSFEDVDSLVSSDCIDEEDMEERAQQERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIY +PIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALW 240
           VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSE LSSEQF+WLCAIM  ATVVKLALW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSESLSSEQFVWLCAIMTFATVVKLALW 240

Query: 241 LYCKSSRNDIVRAYAK 257
           LYCK+SRNDIVRAYAK
Sbjct: 241 LYCKNSRNDIVRAYAK 255

BLAST of Tan0009632 vs. ExPASy TrEMBL
Match: A0A1S3BQ61 (metal tolerance protein 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492484 PE=4 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 5.9e-120
Identity = 234/256 (91.41%), Postives = 245/256 (95.70%), Query Frame = 0

Query: 1   MDGDSDSSPKAALLGGAMKRGGSGRRGRLSRRYSVNSLRSEFISRLPEKVRSRLQDVESP 60
           MDGDSD SPKA LLGG++  GGSGRRGRLSRRYSVNSLRSEFISRLP+K+RS LQDVESP
Sbjct: 1   MDGDSDLSPKAPLLGGSV-NGGSGRRGRLSRRYSVNSLRSEFISRLPDKLRSHLQDVESP 60

Query: 61  YEIDLSQSTGFSRGEKDYYETQLATLKSFEEVDSLVASDCIDEEDSEEQAQQERAMKISN 120
           YEIDLS+STGFSRGEKDYYE QLATLKSFE+VDSLV+SDCIDEED EE+AQQERAMKISN
Sbjct: 61  YEIDLSKSTGFSRGEKDYYERQLATLKSFEDVDSLVSSDCIDEEDMEERAQQERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIY +PIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALW 240
           VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSE LSSEQF+WLCAIM  ATVVKLALW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSESLSSEQFVWLCAIMTFATVVKLALW 240

Query: 241 LYCKSSRNDIVRAYAK 257
           LYCK+SRNDIVRAYAK
Sbjct: 241 LYCKNSRNDIVRAYAK 255

BLAST of Tan0009632 vs. ExPASy TrEMBL
Match: A0A1S3BRU5 (metal tolerance protein 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492484 PE=4 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 5.9e-120
Identity = 234/256 (91.41%), Postives = 245/256 (95.70%), Query Frame = 0

Query: 1   MDGDSDSSPKAALLGGAMKRGGSGRRGRLSRRYSVNSLRSEFISRLPEKVRSRLQDVESP 60
           MDGDSD SPKA LLGG++  GGSGRRGRLSRRYSVNSLRSEFISRLP+K+RS LQDVESP
Sbjct: 1   MDGDSDLSPKAPLLGGSV-NGGSGRRGRLSRRYSVNSLRSEFISRLPDKLRSHLQDVESP 60

Query: 61  YEIDLSQSTGFSRGEKDYYETQLATLKSFEEVDSLVASDCIDEEDSEEQAQQERAMKISN 120
           YEIDLS+STGFSRGEKDYYE QLATLKSFE+VDSLV+SDCIDEED EE+AQQERAMKISN
Sbjct: 61  YEIDLSKSTGFSRGEKDYYERQLATLKSFEDVDSLVSSDCIDEEDMEERAQQERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIY +PIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALW 240
           VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSE LSSEQF+WLCAIM  ATVVKLALW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSESLSSEQFVWLCAIMTFATVVKLALW 240

Query: 241 LYCKSSRNDIVRAYAK 257
           LYCK+SRNDIVRAYAK
Sbjct: 241 LYCKNSRNDIVRAYAK 255

BLAST of Tan0009632 vs. ExPASy TrEMBL
Match: A0A6J1H803 (metal tolerance protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111461318 PE=4 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 7.6e-120
Identity = 231/256 (90.23%), Postives = 243/256 (94.92%), Query Frame = 0

Query: 1   MDGDSDSSPKAALLGGAMKRGGSGRRGRLSRRYSVNSLRSEFISRLPEKVRSRLQDVESP 60
           MDG+ D++PK ALL GA+KR GSGRRGRLSR YSVNSLRSEFISRLPEKV+S LQDVESP
Sbjct: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTGFSRGEKDYYETQLATLKSFEEVDSLVASDCIDEEDSEEQAQQERAMKISN 120
           YEIDLSQST FSRGEKDYYE Q++TLKSFEEVDSLV SDCIDEEDSEE+AQ+ERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQISTLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLR 180
           YANIVLLLLKIYATVRSGSIAIAASTLDSLLDL+AGGILWFTHLYMKRVNIY +PIGKLR
Sbjct: 121 YANIVLLLLKIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLR 180

Query: 181 VQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALW 240
           VQPVGIIVFAAVMATLGFQVLLQA EQLIQDKPSEGLS EQFIWLCAIMI+ATVVKL LW
Sbjct: 181 VQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLVLW 240

Query: 241 LYCKSSRNDIVRAYAK 257
           LYCKSSRNDIVRAYAK
Sbjct: 241 LYCKSSRNDIVRAYAK 256

BLAST of Tan0009632 vs. TAIR 10
Match: AT3G58060.1 (Cation efflux family protein )

HSP 1 Score: 284.3 bits (726), Expect = 1.0e-76
Identity = 153/229 (66.81%), Postives = 185/229 (80.79%), Query Frame = 0

Query: 35  VNSLRSEFISRLPEKVRSRLQDVESPYEIDLSQSTGFSRGEKDYYETQLATLKSFEEVDS 94
           V+S++S F + LP+K+RS++ D E+P  +D+S++ G    EK+YYE QLATLKSFEEV+S
Sbjct: 30  VSSMKSNFFADLPQKLRSKI-DPENPLHLDVSKAAGLKEDEKEYYERQLATLKSFEEVES 89

Query: 95  LVA-------SDCIDEEDSEEQAQQERAMKISNYANIVLLLLKIYATVRSGSIAIAASTL 154
            +A        +  +EED  E+A QE AM+ISN+ANI LL LKIYATV+SGSIAIAASTL
Sbjct: 90  FLARSDEYTIDEKEEEEDRAERAAQELAMQISNWANIFLLALKIYATVKSGSIAIAASTL 149

Query: 155 DSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLRVQPVGIIVFAAVMATLGFQVLLQAVEQ 214
           DSLLDLMAGGILWFTHL MK VNIY YPIGKLRVQPVGII+FAAVMATLGFQVLL A EQ
Sbjct: 150 DSLLDLMAGGILWFTHLSMKNVNIYKYPIGKLRVQPVGIIIFAAVMATLGFQVLLVAAEQ 209

Query: 215 LIQDKPSEGLSSEQFIWLCAIMITATVVKLALWLYCKSSRNDIVRAYAK 257
           LI ++PSE ++  Q IWL +IM++AT +KL LW+YCKSSRN IVRAYAK
Sbjct: 210 LISNEPSEKMNHVQLIWLYSIMLSATAIKLVLWIYCKSSRNHIVRAYAK 257

BLAST of Tan0009632 vs. TAIR 10
Match: AT2G39450.1 (Cation efflux family protein )

HSP 1 Score: 164.9 bits (416), Expect = 9.1e-41
Identity = 89/184 (48.37%), Postives = 125/184 (67.93%), Query Frame = 0

Query: 77  DYYETQLATLKSFEEVDSLVASDCIDEEDSEEQ----AQQERAMKISNYANIVLLLLKIY 136
           DYY+ Q+  L+ F E+D L     +     EEQ      +  A++ISN AN++L   K+Y
Sbjct: 63  DYYQQQVEMLEGFTEMDELAERGFVPGMSKEEQDNLAKSETLAIRISNIANMLLFAAKVY 122

Query: 137 ATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLRVQPVGIIVFAAV 196
           A+V SGS+AI ASTLDSLLDL++G ILWFT   M+  N Y YPIGK R+QP+GI+VFA+V
Sbjct: 123 ASVTSGSLAIIASTLDSLLDLLSGFILWFTAFSMQTPNPYQYPIGKKRMQPLGILVFASV 182

Query: 197 MATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALWLYCKSSRNDIVR 256
           MATLG Q++L+++  ++       L+ EQ  W+  IM++ T+VKL L LYC+S  N+IV+
Sbjct: 183 MATLGLQIILESLRTMLSSHKEFNLTKEQESWVVGIMLSVTLVKLLLVLYCRSFTNEIVK 242

BLAST of Tan0009632 vs. TAIR 10
Match: AT1G79520.1 (Cation efflux family protein )

HSP 1 Score: 158.7 bits (400), Expect = 6.5e-39
Identity = 94/189 (49.74%), Postives = 131/189 (69.31%), Query Frame = 0

Query: 73  RGEKDYYETQLATLKSFEEVDSL----VASDCIDEEDSEEQAQQER-AMKISNYANIVLL 132
           R   +YY+ Q   L+ F E++++      S    EE+ ++ A+ ER A+ ISN AN+VL 
Sbjct: 68  RKVSEYYKQQEKLLEGFNEMETINETGFVSGAPTEEELKKLAKSERLAVHISNAANLVLF 127

Query: 133 LLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLRVQPVGII 192
           + K+YA+V S S+A+ ASTLDSLLDL++G ILWFT   M+  N + YPIGK R+QPVGII
Sbjct: 128 VAKVYASVESRSMAVIASTLDSLLDLLSGFILWFTANAMRTPNNFRYPIGKRRMQPVGII 187

Query: 193 VFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALWLYCKSSR 252
           VFA+VMATLG QV+L++  +L+  K    +SS +  W+  IM +ATVVK  L LYC+S +
Sbjct: 188 VFASVMATLGLQVILEST-RLLVSKNGSHMSSTEEKWMIGIMASATVVKFLLMLYCRSFQ 247

Query: 253 NDIVRAYAK 257
           N+IVRAYA+
Sbjct: 248 NEIVRAYAQ 255

BLAST of Tan0009632 vs. TAIR 10
Match: AT1G79520.2 (Cation efflux family protein )

HSP 1 Score: 158.7 bits (400), Expect = 6.5e-39
Identity = 94/189 (49.74%), Postives = 131/189 (69.31%), Query Frame = 0

Query: 73  RGEKDYYETQLATLKSFEEVDSL----VASDCIDEEDSEEQAQQER-AMKISNYANIVLL 132
           R   +YY+ Q   L+ F E++++      S    EE+ ++ A+ ER A+ ISN AN+VL 
Sbjct: 80  RKVSEYYKQQEKLLEGFNEMETINETGFVSGAPTEEELKKLAKSERLAVHISNAANLVLF 139

Query: 133 LLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLRVQPVGII 192
           + K+YA+V S S+A+ ASTLDSLLDL++G ILWFT   M+  N + YPIGK R+QPVGII
Sbjct: 140 VAKVYASVESRSMAVIASTLDSLLDLLSGFILWFTANAMRTPNNFRYPIGKRRMQPVGII 199

Query: 193 VFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALWLYCKSSR 252
           VFA+VMATLG QV+L++  +L+  K    +SS +  W+  IM +ATVVK  L LYC+S +
Sbjct: 200 VFASVMATLGLQVILEST-RLLVSKNGSHMSSTEEKWMIGIMASATVVKFLLMLYCRSFQ 259

Query: 253 NDIVRAYAK 257
           N+IVRAYA+
Sbjct: 260 NEIVRAYAQ 267

BLAST of Tan0009632 vs. TAIR 10
Match: AT1G16310.1 (Cation efflux family protein )

HSP 1 Score: 156.8 bits (395), Expect = 2.5e-38
Identity = 91/189 (48.15%), Postives = 132/189 (69.84%), Query Frame = 0

Query: 73  RGEKDYYETQLATLKSFEEVDSL----VASDCIDEEDSEEQAQQER-AMKISNYANIVLL 132
           R   +YY+ Q   L+ F E++++     AS    EE+ ++ A+ ER A+ ISN  N+VL 
Sbjct: 95  RRVSEYYKKQERLLEGFNEMETIHENGFASGVPTEEEMKKLAKSERLAVHISNATNLVLF 154

Query: 133 LLKIYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRVNIYNYPIGKLRVQPVGII 192
           + K+YA++ S S+A+ ASTLDSLLDL++G ILWFT   M++ N ++YPIGK R+QPVGII
Sbjct: 155 VAKVYASMESRSMAVIASTLDSLLDLLSGFILWFTANAMRKPNQFHYPIGKRRMQPVGII 214

Query: 193 VFAAVMATLGFQVLLQAVEQLIQDKPSEGLSSEQFIWLCAIMITATVVKLALWLYCKSSR 252
           VFA+VMATLG QVLL++  QL+  K    ++S +  W+  IM++ T+VK  L LYC+  +
Sbjct: 215 VFASVMATLGLQVLLESGRQLVA-KSGIHMNSTEEKWMIGIMVSVTIVKFLLMLYCRGFQ 274

Query: 253 NDIVRAYAK 257
           N+IVRAYA+
Sbjct: 275 NEIVRAYAQ 282

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q10PP83.8e-7668.28Metal tolerance protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP4 PE=2 ... [more]
Q6Z7K51.1e-7561.83Metal tolerance protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP3 PE=2 ... [more]
Q9M2P21.5e-7566.81Putative metal tolerance protein C3 OS=Arabidopsis thaliana OX=3702 GN=MTPC3 PE=... [more]
Q5NA185.2e-4142.64Metal tolerance protein 5 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP5 PE=2 ... [more]
O806321.3e-3948.37Metal tolerance protein 11 OS=Arabidopsis thaliana OX=3702 GN=MTP11 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023516649.13.2e-12091.02metal tolerance protein 4 [Cucurbita pepo subsp. pepo][more]
XP_038878436.15.4e-12091.41metal tolerance protein 4 [Benincasa hispida][more]
XP_022988141.17.1e-12091.02metal tolerance protein 4 [Cucurbita maxima][more]
KAG6590238.17.1e-12090.63Metal tolerance protein 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_008451104.11.2e-11991.41PREDICTED: metal tolerance protein 4 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1JGE13.4e-12091.02metal tolerance protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111485472 PE=4 SV=1[more]
A0A5A7THE55.9e-12091.41Metal tolerance protein 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BQ615.9e-12091.41metal tolerance protein 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492484 PE=... [more]
A0A1S3BRU55.9e-12091.41metal tolerance protein 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492484 PE=... [more]
A0A6J1H8037.6e-12090.23metal tolerance protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111461318 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT3G58060.11.0e-7666.81Cation efflux family protein [more]
AT2G39450.19.1e-4148.37Cation efflux family protein [more]
AT1G79520.16.5e-3949.74Cation efflux family protein [more]
AT1G79520.26.5e-3949.74Cation efflux family protein [more]
AT1G16310.12.5e-3848.15Cation efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027469Cation efflux transmembrane domain superfamilyGENE3D1.20.1510.10Cation efflux protein transmembrane domaincoord: 1..194
e-value: 2.1E-37
score: 130.7
IPR027469Cation efflux transmembrane domain superfamilySUPERFAMILY161111Cation efflux protein transmembrane domain-likecoord: 3..196
IPR002524Cation efflux proteinPFAMPF01545Cation_effluxcoord: 3..195
e-value: 7.3E-27
score: 94.4
IPR002524Cation efflux proteinTIGRFAMTIGR01297TIGR01297coord: 2..274
e-value: 1.1E-33
score: 114.8
IPR027470Cation efflux protein, cytoplasmic domainPFAMPF16916ZT_dimercoord: 200..274
e-value: 7.0E-13
score: 48.5
IPR036837Cation efflux protein, cytoplasmic domain superfamilyGENE3D3.30.70.1350Cation efflux protein, cytoplasmic domaincoord: 199..291
e-value: 4.1E-17
score: 63.9
IPR036837Cation efflux protein, cytoplasmic domain superfamilySUPERFAMILY160240Cation efflux protein cytoplasmic domain-likecoord: 198..276
NoneNo IPR availablePANTHERPTHR43840:SF29METAL TOLERANCE PROTEIN 3coord: 1..293
NoneNo IPR availablePANTHERPTHR43840MITOCHONDRIAL METAL TRANSPORTER 1-RELATEDcoord: 1..293

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0009632.1Tan0009632.1mRNA
Tan0009632.2Tan0009632.2mRNA
Tan0009632.3Tan0009632.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098655 cation transmembrane transport
biological_process GO:0071421 manganese ion transmembrane transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005774 vacuolar membrane
molecular_function GO:0008324 cation transmembrane transporter activity
molecular_function GO:0005384 manganese ion transmembrane transporter activity