Tan0009428 (gene) Snake gourd v1

Overview


Name	Tan0009428
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	WD repeat containing protein
Location	LG09: 63864137 .. 63866651 (+)
RNA-Seq Expression	Tan0009428
Synteny	Tan0009428

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCAACGCTGCCATCACCAAGCACTCAACTTCGCCATTTTCAATGGCGGACTCACGAGCGCCGCTGCCATCTCCATCTTCCCCACCGTCTTTACCTCCCAATGATTTGCTACCCAGACGCTTCAAGGTCATATGGCGCGTTCTATTGATCTCCAATTTCGCCCTTTCAGGTTTACTCTACTATCTCCTCCTTCTGCATTTTCCTTTTCCTCGCATTCTTGATAGGATTTTATTTTTTATTTTTTATTATCCGAATGTCGATTGTTAACTTCCCAGATCCTCAAAATTGGAATGATTCTGAGAAATGGGTAAATGGAATTTGTGTTGGAAAAAATCTGTCTGTTCAATGTTTTAGTTCTGGCAGGCAAGGTGTTTGATGTAATGACTGGGATGAATTTCTTAGTGTAGCTGTGAAATATGTTGTTATTCCCGTGATTTCTTGGTGGAATATTCTTGTTTGTTATGTCGAAATGCTTCTTGTGCGGCGTTGTTGTTGTTAATGACATTGTTTCACACTTCGTCACTTCCAGCCATACTAGATAAAGCTCGTGAAAGAGAGGAACGTTTTCTATTATCTGGTAATATCTCAGATCATTTTCCTTGAGACATGAAAATTTTGAAATTTGAATAGGGAAATGTGTAAGTACAAGCAGCCCTGTTGAAGTTCATATGCCCTGATCTTTATGGTCCAATTATAAAGATGAGATTGCTGAGAAAATGTGTCTGGTTATCGTTAAATGGGCACTCTCGTGGTGTGATACCATATCCACCACAGTATTGTGACATATTTGTAAAAGTTGTCTTGTAAAGATTGGTATTAGTTCTTTCTTAGTTCTTCCAGTTTCTGATTACCTTCTATATGGCTCCACTATTGCTATTTAACTTCACTTGTTAGAGTATGACTTTTAGAAGCACATAAGGCTTTCTTCTAAGAGGTGGGCGATTCTCTGTTTATGAGGGAGGGACATGCATTAGGATTTGTGTGGGAAGTTTGAAAATGAAGGCCCGTTTGATAACTATTTGATTTTTGGTTTTTAGTTTTTTAGCTTATAAACACTATTTTTATCTATGAATTTCTTTGTTTTACTATCTACTTTTACAAATGTTTTCAAAATCCAAACCAAGTTTTGAAAGATAGAAAAAGTAATTTTTGTTTTTAAAATTTGACTAAGAATTCAAATATTTTTCTTAAAAAAGATGAAAACCATTATAGAAAAATTGTGACAATTTTCAAGAACTAAAAACCAAATAGTTATCAAATGGGACCTAAACTTTTAGTTCTACTGAAGAGATCTGAATCAAAGAGAGTAGGAATGCCTATTCAAAGTCATGCTATCCTCCATTTGATTTATCTATGAATTGGCAAAATCAGTTTGGGAAGGCGGTTGTCAATCAGGAAAAAAAAAATTCTCATTTCTATATCTAGAACGTCTCACGTCAGATTCATAACCAATTCAAAAGTCGTACAAAATCTCTTTCAGACTGTGATATGTTCAATTCCACATATATCTCGCAAGATCTTTATAATTTTCAAATCAATGGTCGGGGGCGCAATTGCTTCAAACGGGAGAGAGGGGAAGATATAATCTCCTAAATTTCTTATCTATGTAAGGTACAACTTTAGATGATTCCTTTCTCATGTTGTCCAATGGTTCGAGTTAGTTGAGAATTAGCTTATTAATGAGGAGGTGATAACCAAGATCAGCTATTCAATTTTCCTTTCCTGAAGAACCAGGCAAATTTACAAGTCTTGAATCTGGATTCTGGCACTGTCTTTTCCTCTCTTCTCTTTGAAGCAATTATATCTCTCTTCTTTTCTTTGTATCTATAATCAGTAGAAGAAAAATTTACTGCAGCCACATGACAGAATTTACTTTATTTAACTTCAAACGCGTGTGGCCAACACGAGCATAACTCAACAGTTAAAGCATCCATTTTTTTCCATGAGGTCGGAAGTTTAAATCTCCGCTTCCACCCACATTCGTTGTACTAAAAAAAACTTCAAACACTAGTTTGCAACTGATTTGTATTTCATACATTGCAGCCTATATGTTTGCAAGTGCAAGGAAGAAAGATTTGGGCCAAATGGAGAATGATCAAGCAGAAAAAGATTTGGCTCAACTGGAAAATGATCGAGTAGAAAAACACAGTGATGATGAAATTTCGGAAATACCCTCAACATTTATGGAGAATACCACTAGCATAGTTACAGAGAACATCTCGGAAGATCAACAGCAAGAACCATTCCAGTGGCTGGTTCATGGTTGGAGAGAAAAAATAGGCTTCAGTAAAAAATGAATTGATAGGTGAAAAGCTGATTCTTGAACAGTTCAATGTTCATCTTCTCAAAACTGATTGCTTCTCATCCAGGTAAATATGTTCAGCTGCTGTAAAATTTTCTTTTAGGCGATTTTTAGGAGACTGATTATTGTACTGAAACCTTCCTTAACCGGATATCTTCATGTAGATTTATTTGCAAGTTTGTAACAATAATTTCTAAGAATGTCAAACTTTTT

mRNA sequence

GTCAACGCTGCCATCACCAAGCACTCAACTTCGCCATTTTCAATGGCGGACTCACGAGCGCCGCTGCCATCTCCATCTTCCCCACCGTCTTTACCTCCCAATGATTTGCTACCCAGACGCTTCAAGGTCATATGGCGCGTTCTATTGATCTCCAATTTCGCCCTTTCAGCCTATATGTTTGCAAGTGCAAGGAAGAAAGATTTGGGCCAAATGGAGAATGATCAAGCAGAAAAAGATTTGGCTCAACTGGAAAATGATCGAGTAGAAAAACACAGTGATGATGAAATTTCGGAAATACCCTCAACATTTATGGAGAATACCACTAGCATAGTTACAGAGAACATCTCGGAAGATCAACAGCAAGAACCATTCCAGTGGCTGGTTCATGGTTGGAGAGAAAAAATAGGCTTCAGTAAAAAATGAATTGATAGGTGAAAAGCTGATTCTTGAACAGTTCAATGTTCATCTTCTCAAAACTGATTGCTTCTCATCCAGGTAAATATGTTCAGCTGCTGTAAAATTTTCTTTTAGGCGATTTTTAGGAGACTGATTATTGTACTGAAACCTTCCTTAACCGGATATCTTCATGTAGATTTATTTGCAAGTTTGTAACAATAATTTCTAAGAATGTCAAACTTTTT

Coding sequence (CDS)

ATGGCGGACTCACGAGCGCCGCTGCCATCTCCATCTTCCCCACCGTCTTTACCTCCCAATGATTTGCTACCCAGACGCTTCAAGGTCATATGGCGCGTTCTATTGATCTCCAATTTCGCCCTTTCAGCCTATATGTTTGCAAGTGCAAGGAAGAAAGATTTGGGCCAAATGGAGAATGATCAAGCAGAAAAAGATTTGGCTCAACTGGAAAATGATCGAGTAGAAAAACACAGTGATGATGAAATTTCGGAAATACCCTCAACATTTATGGAGAATACCACTAGCATAGTTACAGAGAACATCTCGGAAGATCAACAGCAAGAACCATTCCAGTGGCTGGTTCATGGTTGGAGAGAAAAAATAGGCTTCAGTAAAAAATGA

Protein sequence

MADSRAPLPSPSSPPSLPPNDLLPRRFKVIWRVLLISNFALSAYMFASARKKDLGQMENDQAEKDLAQLENDRVEKHSDDEISEIPSTFMENTTSIVTENISEDQQQEPFQWLVHGWREKIGFSKK

Homology

[Close]

BLAST of Tan0009428 vs. NCBI nr
Match: KAG6588036.1 (hypothetical protein SDJN03_16601, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021933.1 hypothetical protein SDJN02_15661 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 198.7 bits (504), Expect = 2.9e-47
Identity = 103/126 (81.75%), Postives = 109/126 (86.51%), Query Frame = 0


Query: 1   MADSRAPLPSPSSPPSLPPNDLLPRRFKVIWRVLLISNFALSAYMFASARKKDLGQMEND 60
           MAD RA L S SSPPSLPPND LPRRFKV+WRVLLISNFALSAYMFASARKKDLGQMEND
Sbjct: 1   MADPRASLSSLSSPPSLPPNDQLPRRFKVVWRVLLISNFALSAYMFASARKKDLGQMEND 60

Query: 61  QAEKDLAQLENDRVEKHSDDEISEIPSTFMENTTSIVTENISEDQQQEPFQWLVHGWREK 120
           +AEKDL QLEND +EK SD EI EIPSTFM +TT IVTENISEDQ Q+PF W+V GWREK
Sbjct: 61  EAEKDLGQLENDGIEKCSDSEIMEIPSTFMVDTTGIVTENISEDQHQKPFHWIVDGWREK 120

Query: 121 IGFSKK 127
            G SKK
Sbjct: 121 TGSSKK 126

[Close]

BLAST of Tan0009428 vs. NCBI nr
Match: XP_023530326.1 (uncharacterized protein LOC111792930 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 196.4 bits (498), Expect = 1.4e-46
Identity = 102/126 (80.95%), Postives = 108/126 (85.71%), Query Frame = 0


Query: 1   MADSRAPLPSPSSPPSLPPNDLLPRRFKVIWRVLLISNFALSAYMFASARKKDLGQMEND 60
           MAD RA L S SSPPSLPPND LPRRFKV+WRVLLISNFALSAYMFASARKKDLGQMEND
Sbjct: 1   MADPRASLSSLSSPPSLPPNDQLPRRFKVVWRVLLISNFALSAYMFASARKKDLGQMEND 60

Query: 61  QAEKDLAQLENDRVEKHSDDEISEIPSTFMENTTSIVTENISEDQQQEPFQWLVHGWREK 120
           +AEKDL QLEND +EK SD EI EIPST M +TT IVTENISEDQ Q+PF W+V GWREK
Sbjct: 61  EAEKDLGQLENDGIEKCSDSEIMEIPSTLMVDTTGIVTENISEDQHQKPFHWIVDGWREK 120

Query: 121 IGFSKK 127
            G SKK
Sbjct: 121 TGSSKK 126

[Close]

BLAST of Tan0009428 vs. NCBI nr
Match: XP_022968528.1 (uncharacterized protein LOC111467730 isoform X1 [Cucurbita maxima])

HSP 1 Score: 194.9 bits (494), Expect = 4.2e-46
Identity = 101/126 (80.16%), Postives = 108/126 (85.71%), Query Frame = 0


Query: 1   MADSRAPLPSPSSPPSLPPNDLLPRRFKVIWRVLLISNFALSAYMFASARKKDLGQMEND 60
           MAD RA L S SSPPSLPPND LPRRFKV+WRVLLISNFALSAYMFASARKKDLGQME+D
Sbjct: 1   MADPRASLSSLSSPPSLPPNDQLPRRFKVVWRVLLISNFALSAYMFASARKKDLGQMESD 60

Query: 61  QAEKDLAQLENDRVEKHSDDEISEIPSTFMENTTSIVTENISEDQQQEPFQWLVHGWREK 120
           +AEKDL QLEND +EK SD EI EIPSTFM +TT IVTENI EDQ Q+PF W+V GWREK
Sbjct: 61  EAEKDLGQLENDGIEKCSDSEIMEIPSTFMVDTTGIVTENIPEDQHQKPFHWIVDGWREK 120

Query: 121 IGFSKK 127
            G SKK
Sbjct: 121 TGSSKK 126

[Close]

BLAST of Tan0009428 vs. NCBI nr
Match: XP_022926648.1 (uncharacterized protein LOC111433716 [Cucurbita moschata])

HSP 1 Score: 193.0 bits (489), Expect = 1.6e-45
Identity = 101/126 (80.16%), Postives = 108/126 (85.71%), Query Frame = 0


Query: 1   MADSRAPLPSPSSPPSLPPNDLLPRRFKVIWRVLLISNFALSAYMFASARKKDLGQMEND 60
           MAD RA L S SSPPSL PND LPRRFKV+WRVLLISNFALSAYMFASARKKDLGQMEN+
Sbjct: 1   MADPRASLSSLSSPPSLLPNDQLPRRFKVVWRVLLISNFALSAYMFASARKKDLGQMENN 60

Query: 61  QAEKDLAQLENDRVEKHSDDEISEIPSTFMENTTSIVTENISEDQQQEPFQWLVHGWREK 120
           +AEKDL QLEND +EK SD EI EIPSTFM +TT IVTENISEDQ Q+PF W+V GWREK
Sbjct: 61  EAEKDLGQLENDGIEKCSDSEIMEIPSTFMVDTTGIVTENISEDQHQKPFHWIVDGWREK 120

Query: 121 IGFSKK 127
            G SKK
Sbjct: 121 TGSSKK 126

[Close]

BLAST of Tan0009428 vs. NCBI nr
Match: XP_004135711.1 (uncharacterized protein LOC101210247 isoform X1 [Cucumis sativus])

HSP 1 Score: 152.9 bits (385), Expect = 1.8e-33
Identity = 85/127 (66.93%), Postives = 95/127 (74.80%), Query Frame = 0


Query: 1   MADSRAPLPSPSSPPSLPPNDLLPRRFKVIWRVLLISNFALSAYMFASARKKDLGQMEND 60
           M D RAPLPS SS   LPPND LP+RFKV+WRVLLISNFALSAYMFASARK+   Q+END
Sbjct: 1   MTDPRAPLPSSSSSTYLPPNDSLPKRFKVLWRVLLISNFALSAYMFASARKRHFNQLEND 60

Query: 61  QAEKDLAQLENDRVEKHSDDE-ISEIPSTFMENTTSIVTENISEDQQQEPFQWLVHGWRE 120
           Q            VEK SD E I+EIPST M +TT IVTEN+ EDQQQEP Q +V GWRE
Sbjct: 61  Q------------VEKQSDSEIITEIPSTIMVDTTGIVTENVLEDQQQEPDQGVVSGWRE 115

Query: 121 KIGFSKK 127
           K+GFS+K
Sbjct: 121 KLGFSRK 115

[Close]

BLAST of Tan0009428 vs. ExPASy TrEMBL
Match: A0A6J1HYA0 (uncharacterized protein LOC111467730 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467730 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.0e-46
Identity = 101/126 (80.16%), Postives = 108/126 (85.71%), Query Frame = 0


Query: 1   MADSRAPLPSPSSPPSLPPNDLLPRRFKVIWRVLLISNFALSAYMFASARKKDLGQMEND 60
           MAD RA L S SSPPSLPPND LPRRFKV+WRVLLISNFALSAYMFASARKKDLGQME+D
Sbjct: 1   MADPRASLSSLSSPPSLPPNDQLPRRFKVVWRVLLISNFALSAYMFASARKKDLGQMESD 60

Query: 61  QAEKDLAQLENDRVEKHSDDEISEIPSTFMENTTSIVTENISEDQQQEPFQWLVHGWREK 120
           +AEKDL QLEND +EK SD EI EIPSTFM +TT IVTENI EDQ Q+PF W+V GWREK
Sbjct: 61  EAEKDLGQLENDGIEKCSDSEIMEIPSTFMVDTTGIVTENIPEDQHQKPFHWIVDGWREK 120

Query: 121 IGFSKK 127
            G SKK
Sbjct: 121 TGSSKK 126

[Close]

BLAST of Tan0009428 vs. ExPASy TrEMBL
Match: A0A6J1EIP0 (uncharacterized protein LOC111433716 OS=Cucurbita moschata OX=3662 GN=LOC111433716 PE=4 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 7.7e-46
Identity = 101/126 (80.16%), Postives = 108/126 (85.71%), Query Frame = 0


Query: 1   MADSRAPLPSPSSPPSLPPNDLLPRRFKVIWRVLLISNFALSAYMFASARKKDLGQMEND 60
           MAD RA L S SSPPSL PND LPRRFKV+WRVLLISNFALSAYMFASARKKDLGQMEN+
Sbjct: 1   MADPRASLSSLSSPPSLLPNDQLPRRFKVVWRVLLISNFALSAYMFASARKKDLGQMENN 60

Query: 61  QAEKDLAQLENDRVEKHSDDEISEIPSTFMENTTSIVTENISEDQQQEPFQWLVHGWREK 120
           +AEKDL QLEND +EK SD EI EIPSTFM +TT IVTENISEDQ Q+PF W+V GWREK
Sbjct: 61  EAEKDLGQLENDGIEKCSDSEIMEIPSTFMVDTTGIVTENISEDQHQKPFHWIVDGWREK 120

Query: 121 IGFSKK 127
            G SKK
Sbjct: 121 TGSSKK 126

[Close]

BLAST of Tan0009428 vs. ExPASy TrEMBL
Match: A0A0A0M1X9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G587470 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 8.8e-34
Identity = 85/127 (66.93%), Postives = 95/127 (74.80%), Query Frame = 0


Query: 1   MADSRAPLPSPSSPPSLPPNDLLPRRFKVIWRVLLISNFALSAYMFASARKKDLGQMEND 60
           M D RAPLPS SS   LPPND LP+RFKV+WRVLLISNFALSAYMFASARK+   Q+END
Sbjct: 1   MTDPRAPLPSSSSSTYLPPNDSLPKRFKVLWRVLLISNFALSAYMFASARKRHFNQLEND 60

Query: 61  QAEKDLAQLENDRVEKHSDDE-ISEIPSTFMENTTSIVTENISEDQQQEPFQWLVHGWRE 120
           Q            VEK SD E I+EIPST M +TT IVTEN+ EDQQQEP Q +V GWRE
Sbjct: 61  Q------------VEKQSDSEIITEIPSTIMVDTTGIVTENVLEDQQQEPDQGVVSGWRE 115

Query: 121 KIGFSKK 127
           K+GFS+K
Sbjct: 121 KLGFSRK 115

[Close]

BLAST of Tan0009428 vs. ExPASy TrEMBL
Match: A0A1S3BR82 (uncharacterized protein LOC103492320 OS=Cucumis melo OX=3656 GN=LOC103492320 PE=4 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.2e-32
Identity = 82/127 (64.57%), Postives = 94/127 (74.02%), Query Frame = 0


Query: 1   MADSRAPLPSPSSPPSLPPNDLLPRRFKVIWRVLLISNFALSAYMFASARKKDLGQMEND 60
           M  SR PLPS SS   LPPND LP+RFKV+WR+LLISNFALSAYMFASARK+   ++END
Sbjct: 1   MTASREPLPSSSSSTYLPPNDSLPKRFKVLWRILLISNFALSAYMFASARKRHFNELEND 60

Query: 61  QAEKDLAQLENDRVEKHSDDE-ISEIPSTFMENTTSIVTENISEDQQQEPFQWLVHGWRE 120
           Q            VEK SD E I+EIPST M +TT IVTEN+ EDQQQEP Q +V GWRE
Sbjct: 61  Q------------VEKQSDSEIITEIPSTIMVDTTGIVTENVLEDQQQEPNQGVVSGWRE 115

Query: 121 KIGFSKK 127
           K+GFS+K
Sbjct: 121 KLGFSRK 115

[Close]

BLAST of Tan0009428 vs. ExPASy TrEMBL
Match: A0A6J1D315 (uncharacterized protein LOC111016544 OS=Momordica charantia OX=3673 GN=LOC111016544 PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.7e-30
Identity = 79/115 (68.70%), Postives = 88/115 (76.52%), Query Frame = 0


Query: 1   MADSRAPLPSPSS-PPSLPPNDLLPRRFKVIWRVLLISNFALSAYMFASARKKDLGQMEN 60
           M D RAPLPS SS PP LPPNDLLPRRFKVIWRVLLISNFAL+AYMFA+ARKKDLG+ME 
Sbjct: 1   MTDPRAPLPSSSSPPPPLPPNDLLPRRFKVIWRVLLISNFALAAYMFANARKKDLGRMEK 60

Query: 61  DQAEKDLAQLENDRVEKHSDDEI-SEIPSTFMENTTSIVTENISEDQQQEPFQWL 114
           D             VEKHSD E+ + IPST M +TT+I  ENISED Q+EPFQW+
Sbjct: 61  DP------------VEKHSDGEVMTNIPSTLMVDTTAIAAENISEDHQREPFQWM 103

The following BLAST results are available for this feature:

BLAST of Tan0009428 vs.


Match Name	E-value	Identity	Description

BLAST of Tan0009428 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6588036.1	2.9e-47	81.75	hypothetical protein SDJN03_16601, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023530326.1	1.4e-46	80.95	uncharacterized protein LOC111792930 [Cucurbita pepo subsp. pepo]	[more]
XP_022968528.1	4.2e-46	80.16	uncharacterized protein LOC111467730 isoform X1 [Cucurbita maxima]	[more]
XP_022926648.1	1.6e-45	80.16	uncharacterized protein LOC111433716 [Cucurbita moschata]	[more]
XP_004135711.1	1.8e-33	66.93	uncharacterized protein LOC101210247 isoform X1 [Cucumis sativus]	[more]

BLAST of Tan0009428 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HYA0	2.0e-46	80.16	uncharacterized protein LOC111467730 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1EIP0	7.7e-46	80.16	uncharacterized protein LOC111433716 OS=Cucurbita moschata OX=3662 GN=LOC1114337...	[more]
A0A0A0M1X9	8.8e-34	66.93	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G587470 PE=4 SV=1	[more]
A0A1S3BR82	2.2e-32	64.57	uncharacterized protein LOC103492320 OS=Cucumis melo OX=3656 GN=LOC103492320 PE=...	[more]
A0A6J1D315	2.7e-30	68.70	uncharacterized protein LOC111016544 OS=Momordica charantia OX=3673 GN=LOC111016...	[more]

BLAST of Tan0009428 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 52..79
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 57..89
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 57..84
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR34364:SF9	SUBFAMILY NOT NAMED	coord: 12..118
None	No IPR available	PANTHER	PTHR34364	WAS/WASL-INTERACTING FAMILY PROTEIN	coord: 12..118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0009428.1	Tan0009428.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane