Tan0009250 (gene) Snake gourd v1

Overview
NameTan0009250
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPentatricopeptide repeat-containing protein
LocationLG08: 10937757 .. 10940392 (+)
RNA-Seq ExpressionTan0009250
SyntenyTan0009250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGAGAATAAAAGCTTCTCCACATCTCATTTCCCGCTATCTCAATTTCGACAAAACACATTCAAATCCAAATCCCAATCCCAATCGTCAAGCTCTGTCCCAAAAGCTCGTCGGCTTCTTGCAACAATGTCTAACCATCAAACAATTGAAACAAATTCACACCCAGATGCTCACGAACTCAATTCACAAACCCAATTCGTTTCTCTACAAGATCGTCGACCTCAAAGATTTCGCCTATGCGTCGGATTTCTTCTCGAAGATTCTCGAGCCCACCGAGTACTCTTTCAATGTCATGATTCGTGGCCTCTCGACGACTTGGAATAAGTCTTCTCTTGCCCTTGAATTCTATTCTCGAATGAAATTTCTGGGCCTGAAACCCAATAAACTTACGTACCCGTTTCTGTTTATTGCGTGTTCGAATCTTTTGGCTGTTGGACATGGGCGGGTGGGGCATTCTTCTGTGATTAAACGTGGGTTGGATGAGGATGGTCATGTGAGTCATTCTTTGATTACGATGTACGCGAGGTGTGGGGATCTGGGTGACGCCCGGAAGGTGTTTGATGAAATTCCTGAGAGGGATTTGGTTTCGTGGAACTCTATGATTTCGGGTTACTCGAAGATGAGGTATGCTGGAGAGGCTGTGGGGTTGTTTAGAGAGATGATGGAAGCTGGGTTTGAGCCCAATGAGATGACTTTAGTTAGTGTCTTGGGGGCGTGTGGAGAGCTGGGGGATTTGAAACTTGGTACTTGGGTGGAGGAGTTTGTGGTGGAGAATAAGATGGTGTTGAATTCTTTTATGGGTTCTGCATTGATCCATATGTATGGGAAATGTGGGGATTTAGTGTCAGCAAGGAGGATCTTTGACTCTATGAGGAAGAAAGATAAAGTTACGTGGAATGCCATGATTACAGGGCAAGTGGATGACCATCTTCTCTTTGAATCAATACTTTTGGATATTGTTATTAATAGATCATCAGTTGAATCTATGTTTTAGTCCCTATTGATTAAAAAGTTTCAATCTGGTTCCTTACGTTTAAAATTTTTTAGTTATGCCATTTTTGTTAGTGGGTGTTAAATATGATTCATGATTAGCTTATGTGACATAAATACTGAGTAAATTTGCAAAAACTTGGAGGAAGAATCGAGCCACTTGGGAGAAGAAAAGTATAAACTTTTTGACCACTTGGATGTTTGGAGAAGAAATTAAATTTTTTTCGCTCTTAGGTGGCATTTCCTCCTCTACATCTATGGCAACTCACTCGGTAGCATGTTGTGTGAGGCTACCATTAGTCACATTTAATATCCGTTGATAAAAATTTAAAGGCAAGAAACTAAATTGAAACTTTTTAAACAATAGGAACTGAATAAAAAAAAAACGCTCAAACATTAGTGCAGTCTAGATAATAATGTCTTTCTCTATTCCAAATTTGATGGAATCTTTTTTTTTCTTCAATCAATCAATTACAGATATGCGCAGAATGGGATGTCAGAGGAAGCTATTAAATTGTTCCAAGACATGAGAATGAGTAGCACCACTCCAGATCAAATCACATTGATAGGAATGCTCTCTGCCTGTGCCTCCATTGGAGCTCTTGACTTGGGGAAGCAAGTAGAGATTTATGCCTCAGAAAGAGGCTTGCGAGATGATATTTATGTTGCTACTGCATTAGTTGACATGTACGCGAAATGTGGAAGCTTGGATAATGCATTTAGAGTTTTCGATGGCATGCCTCGGAAAAACGAGGTCTCCTGGAATGCGATGATATCTGCTCTGGCTTTCCATGGCCAAGCTCAAGAAGCTTTGGCATTGTTCAAATCCATGATCAAGGAGGGTGGAGCTGCTTCCCCCAATGACATCACATTTGTAGGAGTACTTTCTGCTTGCGTGCACGCAGGATTAGTCGATGAAGGACGTAGGTTGTTTCACATGATGAGCTCATCATTCGGATTGGTGCCTAAAATCGAGCACTACTCTTGTATGGTGGACCTTTTGTCACGGGCAGGACATCTCGAAGAAGCTTGGGACTTTGTCGAGAAGATGCCTGAAAAACCAGACGAAGTCATTCTCGGAGCATTGCTTGGTGCCTGTCAGAAGCGCAAGAACGTTGATATTAGTGAGCGTGTGATGAAGCTACTCCTTGAGTTGGAACCTTCAAATTCTGGAAATTACGTAATCTCGTCGAGATTATATGCAAATTTGAGAAGATGGGACGACTCAGCTAGAATGAGAATGATGATGAAGCAAAAGGGTGTTTCAAAGACTCCTGGTTGCAGTTGGATTGATATCGATTCACAACTTCATGAGTTTCATGCTGGCGATGTTTTACACCAAGAGTGGATTGAAATCCATCAAATCCTAGAATTGTTGATAGATGATTTGAGGAGGGAAGGTTATATTCCTAACACCAGTCTTCTTTAGCTAGACTAATTTTCCTTCATGTTCATTGTTTTATTCCATAAGATGAAGTCCAAACACAAATTTTACACCGAGGAATGGACGTATACAACCTAAGCAACTGCTCAGGAATTGTATTTAAACCAGTCAAAGGCCGAGAAGAAAGCTGTTCGAGGCCGAAAAGATCTGTAAGATGGCTCTACAAATCTTCATTCTTCTTTAGAATACTTTAAATCAAT

mRNA sequence

ATGCTGAGAATAAAAGCTTCTCCACATCTCATTTCCCGCTATCTCAATTTCGACAAAACACATTCAAATCCAAATCCCAATCCCAATCGTCAAGCTCTGTCCCAAAAGCTCGTCGGCTTCTTGCAACAATGTCTAACCATCAAACAATTGAAACAAATTCACACCCAGATGCTCACGAACTCAATTCACAAACCCAATTCGTTTCTCTACAAGATCGTCGACCTCAAAGATTTCGCCTATGCGTCGGATTTCTTCTCGAAGATTCTCGAGCCCACCGAGTACTCTTTCAATGTCATGATTCGTGGCCTCTCGACGACTTGGAATAAGTCTTCTCTTGCCCTTGAATTCTATTCTCGAATGAAATTTCTGGGCCTGAAACCCAATAAACTTACGTACCCGTTTCTGTTTATTGCGTGTTCGAATCTTTTGGCTGTTGGACATGGGCGGGTGGGGCATTCTTCTGTGATTAAACGTGGGTTGGATGAGGATGGTCATGTGAGTCATTCTTTGATTACGATGTACGCGAGGTGTGGGGATCTGGGTGACGCCCGGAAGGTGTTTGATGAAATTCCTGAGAGGGATTTGGTTTCGTGGAACTCTATGATTTCGGGTTACTCGAAGATGAGGTATGCTGGAGAGGCTGTGGGGTTGTTTAGAGAGATGATGGAAGCTGGGTTTGAGCCCAATGAGATGACTTTAGTTAGTGTCTTGGGGGCGTGTGGAGAGCTGGGGGATTTGAAACTTGGTACTTGGGTGGAGGAGTTTGTGGTGGAGAATAAGATGGTGTTGAATTCTTTTATGGGTTCTGCATTGATCCATATGTATGGGAAATGTGGGGATTTAGTGTCAGCAAGGAGGATCTTTGACTCTATGAGGAAGAAAGATAAAGTTACGTGGAATGCCATGATTACAGGATATGCGCAGAATGGGATGTCAGAGGAAGCTATTAAATTGTTCCAAGACATGAGAATGAGTAGCACCACTCCAGATCAAATCACATTGATAGGAATGCTCTCTGCCTGTGCCTCCATTGGAGCTCTTGACTTGGGGAAGCAAGTAGAGATTTATGCCTCAGAAAGAGGCTTGCGAGATGATATTTATGTTGCTACTGCATTAGTTGACATGTACGCGAAATGTGGAAGCTTGGATAATGCATTTAGAGTTTTCGATGGCATGCCTCGGAAAAACGAGGTCTCCTGGAATGCGATGATATCTGCTCTGGCTTTCCATGGCCAAGCTCAAGAAGCTTTGGCATTGTTCAAATCCATGATCAAGGAGGGTGGAGCTGCTTCCCCCAATGACATCACATTTGTAGGAGTACTTTCTGCTTGCGTGCACGCAGGATTAGTCGATGAAGGACGTAGGTTGTTTCACATGATGAGCTCATCATTCGGATTGGTGCCTAAAATCGAGCACTACTCTTGTATGGTGGACCTTTTGTCACGGGCAGGACATCTCGAAGAAGCTTGGGACTTTGTCGAGAAGATGCCTGAAAAACCAGACGAAGTCATTCTCGGAGCATTGCTTGGTGCCTGTCAGAAGCGCAAGAACGTTGATATTAGTGAGCGTGTGATGAAGCTACTCCTTGAGTTGGAACCTTCAAATTCTGGAAATTACGTAATCTCGTCGAGATTATATGCAAATTTGAGAAGATGGGACGACTCAGCTAGAATGAGAATGATGATGAAGCAAAAGGGTGTTTCAAAGACTCCTGGTTGCAGTTGGATTGATATCGATTCACAACTTCATGAGTTTCATGCTGGCGATGTTTTACACCAAGAGTGGATTGAAATCCATCAAATCCTAGAATTGTTGATAGATGATTTGAGGAGGGAAGGTTATATTCCTAACACCAGTCTTCTTTAGCTAGACTAATTTTCCTTCATGTTCATTGTTTTATTCCATAAGATGAAGTCCAAACACAAATTTTACACCGAGGAATGGACGTATACAACCTAAGCAACTGCTCAGGAATTGTATTTAAACCAGTCAAAGGCCGAGAAGAAAGCTGTTCGAGGCCGAAAAGATCTGTAAGATGGCTCTACAAATCTTCATTCTTCTTTAGAATACTTTAAATCAAT

Coding sequence (CDS)

ATGCTGAGAATAAAAGCTTCTCCACATCTCATTTCCCGCTATCTCAATTTCGACAAAACACATTCAAATCCAAATCCCAATCCCAATCGTCAAGCTCTGTCCCAAAAGCTCGTCGGCTTCTTGCAACAATGTCTAACCATCAAACAATTGAAACAAATTCACACCCAGATGCTCACGAACTCAATTCACAAACCCAATTCGTTTCTCTACAAGATCGTCGACCTCAAAGATTTCGCCTATGCGTCGGATTTCTTCTCGAAGATTCTCGAGCCCACCGAGTACTCTTTCAATGTCATGATTCGTGGCCTCTCGACGACTTGGAATAAGTCTTCTCTTGCCCTTGAATTCTATTCTCGAATGAAATTTCTGGGCCTGAAACCCAATAAACTTACGTACCCGTTTCTGTTTATTGCGTGTTCGAATCTTTTGGCTGTTGGACATGGGCGGGTGGGGCATTCTTCTGTGATTAAACGTGGGTTGGATGAGGATGGTCATGTGAGTCATTCTTTGATTACGATGTACGCGAGGTGTGGGGATCTGGGTGACGCCCGGAAGGTGTTTGATGAAATTCCTGAGAGGGATTTGGTTTCGTGGAACTCTATGATTTCGGGTTACTCGAAGATGAGGTATGCTGGAGAGGCTGTGGGGTTGTTTAGAGAGATGATGGAAGCTGGGTTTGAGCCCAATGAGATGACTTTAGTTAGTGTCTTGGGGGCGTGTGGAGAGCTGGGGGATTTGAAACTTGGTACTTGGGTGGAGGAGTTTGTGGTGGAGAATAAGATGGTGTTGAATTCTTTTATGGGTTCTGCATTGATCCATATGTATGGGAAATGTGGGGATTTAGTGTCAGCAAGGAGGATCTTTGACTCTATGAGGAAGAAAGATAAAGTTACGTGGAATGCCATGATTACAGGATATGCGCAGAATGGGATGTCAGAGGAAGCTATTAAATTGTTCCAAGACATGAGAATGAGTAGCACCACTCCAGATCAAATCACATTGATAGGAATGCTCTCTGCCTGTGCCTCCATTGGAGCTCTTGACTTGGGGAAGCAAGTAGAGATTTATGCCTCAGAAAGAGGCTTGCGAGATGATATTTATGTTGCTACTGCATTAGTTGACATGTACGCGAAATGTGGAAGCTTGGATAATGCATTTAGAGTTTTCGATGGCATGCCTCGGAAAAACGAGGTCTCCTGGAATGCGATGATATCTGCTCTGGCTTTCCATGGCCAAGCTCAAGAAGCTTTGGCATTGTTCAAATCCATGATCAAGGAGGGTGGAGCTGCTTCCCCCAATGACATCACATTTGTAGGAGTACTTTCTGCTTGCGTGCACGCAGGATTAGTCGATGAAGGACGTAGGTTGTTTCACATGATGAGCTCATCATTCGGATTGGTGCCTAAAATCGAGCACTACTCTTGTATGGTGGACCTTTTGTCACGGGCAGGACATCTCGAAGAAGCTTGGGACTTTGTCGAGAAGATGCCTGAAAAACCAGACGAAGTCATTCTCGGAGCATTGCTTGGTGCCTGTCAGAAGCGCAAGAACGTTGATATTAGTGAGCGTGTGATGAAGCTACTCCTTGAGTTGGAACCTTCAAATTCTGGAAATTACGTAATCTCGTCGAGATTATATGCAAATTTGAGAAGATGGGACGACTCAGCTAGAATGAGAATGATGATGAAGCAAAAGGGTGTTTCAAAGACTCCTGGTTGCAGTTGGATTGATATCGATTCACAACTTCATGAGTTTCATGCTGGCGATGTTTTACACCAAGAGTGGATTGAAATCCATCAAATCCTAGAATTGTTGATAGATGATTTGAGGAGGGAAGGTTATATTCCTAACACCAGTCTTCTTTAG

Protein sequence

MLRIKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTNSIHKPNSFLYKIVDLKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSRMKFLGLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGDLGDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTWNAMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGLRDDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALFKSMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLLSRAGHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSGNYVISSRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQILELLIDDLRREGYIPNTSLL
Homology
BLAST of Tan0009250 vs. ExPASy Swiss-Prot
Match: O64705 (Pentatricopeptide repeat-containing protein At2g34400 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E23 PE=3 SV=2)

HSP 1 Score: 677.9 bits (1748), Expect = 1.1e-193
Identity = 340/613 (55.46%), Postives = 439/613 (71.62%), Query Frame = 0

Query: 4   IKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTNSIH 63
           IK      S Y  F K          R  L +  +  L++C+++ QL+QI  QML +S+ 
Sbjct: 7   IKPEKLAFSIYRQFPKFKPRQFEEARRGDLERDFLFLLKKCISVNQLRQIQAQMLLHSVE 66

Query: 64  KPNSFLYKIVDLKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSRMKFL 123
           KPN  + K V+L DF Y+S  FS   EP  YSFN MIRGL+ TWN    AL  Y RMKF 
Sbjct: 67  KPNFLIPKAVELGDFNYSSFLFSVTEEPNHYSFNYMIRGLTNTWNDHEAALSLYRRMKFS 126

Query: 124 GLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGDLGDA 183
           GLKP+K TY F+FIAC+ L  +G GR  HSS+ K GL+ D H++HSLI MYA+CG +G A
Sbjct: 127 GLKPDKFTYNFVFIACAKLEEIGVGRSVHSSLFKVGLERDVHINHSLIMMYAKCGQVGYA 186

Query: 184 RKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGEL 243
           RK+FDEI ERD VSWNSMISGYS+  YA +A+ LFR+M E GFEP+E TLVS+LGAC  L
Sbjct: 187 RKLFDEITERDTVSWNSMISGYSEAGYAKDAMDLFRKMEEEGFEPDERTLVSMLGACSHL 246

Query: 244 GDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTWNAMI 303
           GDL+ G  +EE  +  K+ L++F+GS LI MYGKCGDL SARR+F+ M KKD+V W AMI
Sbjct: 247 GDLRTGRLLEEMAITKKIGLSTFLGSKLISMYGKCGDLDSARRVFNQMIKKDRVAWTAMI 306

Query: 304 TGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGLR 363
           T Y+QNG S EA KLF +M  +  +PD  TL  +LSAC S+GAL+LGKQ+E +ASE  L+
Sbjct: 307 TVYSQNGKSSEAFKLFFEMEKTGVSPDAGTLSTVLSACGSVGALELGKQIETHASELSLQ 366

Query: 364 DDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALFKSM 423
            +IYVAT LVDMY KCG ++ A RVF+ MP KNE +WNAMI+A A  G A+EAL LF  M
Sbjct: 367 HNIYVATGLVDMYGKCGRVEEALRVFEAMPVKNEATWNAMITAYAHQGHAKEALLLFDRM 426

Query: 424 IKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLLSRA 483
                +  P+DITF+GVLSACVHAGLV +G R FH MSS FGLVPKIEHY+ ++DLLSRA
Sbjct: 427 -----SVPPSDITFIGVLSACVHAGLVHQGCRYFHEMSSMFGLVPKIEHYTNIIDLLSRA 486

Query: 484 GHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLEL-EPSNSGNYVIS 543
           G L+EAW+F+E+ P KPDE++L A+LGAC KRK+V I E+ M++L+E+ E  N+GNYVIS
Sbjct: 487 GMLDEAWEFMERFPGKPDEIMLAAILGACHKRKDVAIREKAMRMLMEMKEAKNAGNYVIS 546

Query: 544 SRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHAG-DVLHQEWIEIHQI 603
           S + A+++ WD+SA+MR +M+ +GV KTPGCSWI+I+ +L EF AG D L     +   +
Sbjct: 547 SNVLADMKMWDESAKMRALMRDRGVVKTPGCSWIEIEGELMEFLAGSDYLQCGREDSGSL 606

Query: 604 LELLIDDLRREGY 615
            +LL+++++RE Y
Sbjct: 607 FDLLVEEMKRERY 614

BLAST of Tan0009250 vs. ExPASy Swiss-Prot
Match: Q9LN01 (Pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H12 PE=2 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 3.0e-124
Identity = 238/622 (38.26%), Postives = 365/622 (58.68%), Query Frame = 0

Query: 38  VGFLQQCLTIKQLKQIHTQMLTNSIHKPNSFLYKIVD-------LKDFAYASDFFSKILE 97
           +  L  C T++ L+ IH QM+   +H  N  L K+++        +   YA   F  I E
Sbjct: 37  LSLLHNCKTLQSLRIIHAQMIKIGLHNTNYALSKLIEFCILSPHFEGLPYAISVFKTIQE 96

Query: 98  PTEYSFNVMIRGLSTTWNKSSLALEFYSRMKFLGLKPNKLTYPFLFIACSNLLAVGHGRV 157
           P    +N M RG + + +  S AL+ Y  M  LGL PN  T+PF+  +C+   A   G+ 
Sbjct: 97  PNLLIWNTMFRGHALSSDPVS-ALKLYVCMISLGLLPNSYTFPFVLKSCAKSKAFKEGQQ 156

Query: 158 GHSSVIKRGLDEDGHVSHSLITMYARCGDLGDARKV------------------------ 217
            H  V+K G D D +V  SLI+MY + G L DA KV                        
Sbjct: 157 IHGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPHRDVVSYTALIKGYASRGY 216

Query: 218 -------FDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGA 277
                  FDEIP +D+VSWN+MISGY++     EA+ LF++MM+    P+E T+V+V+ A
Sbjct: 217 IENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEALELFKDMMKTNVRPDESTMVTVVSA 276

Query: 278 CGELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTW 337
           C + G ++LG  V  ++ ++    N  + +ALI +Y KCG+L +A  +F+ +  KD ++W
Sbjct: 277 CAQSGSIELGRQVHLWIDDHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKDVISW 336

Query: 338 NAMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASE 397
           N +I GY    + +EA+ LFQ+M  S  TP+ +T++ +L ACA +GA+D+G+ + +Y  +
Sbjct: 337 NTLIGGYTHMNLYKEALLLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDK 396

Query: 398 R--GLRDDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEAL 457
           R  G+ +   + T+L+DMYAKCG ++ A +VF+ +  K+  SWNAMI   A HG+A  + 
Sbjct: 397 RLKGVTNASSLRTSLIDMYAKCGDIEAAHQVFNSILHKSLSSWNAMIFGFAMHGRADASF 456

Query: 458 ALFKSMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMV 517
            LF  M K G    P+DITFVG+LSAC H+G++D GR +F  M+  + + PK+EHY CM+
Sbjct: 457 DLFSRMRKIG--IQPDDITFVGLLSACSHSGMLDLGRHIFRTMTQDYKMTPKLEHYGCMI 516

Query: 518 DLLSRAGHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSG 577
           DLL  +G  +EA + +  M  +PD VI  +LL AC+   NV++ E   + L+++EP N G
Sbjct: 517 DLLGHSGLFKEAEEMINMMEMEPDGVIWCSLLKACKMHGNVELGESFAENLIKIEPENPG 576

Query: 578 NYVISSRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIE 620
           +YV+ S +YA+  RW++ A+ R ++  KG+ K PGCS I+IDS +HEF  GD  H    E
Sbjct: 577 SYVLLSNIYASAGRWNEVAKTRALLNDKGMKKVPGCSSIEIDSVVHEFIIGDKFHPRNRE 636

BLAST of Tan0009250 vs. ExPASy Swiss-Prot
Match: O82380 (Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H33 PE=2 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 9.1e-121
Identity = 234/652 (35.89%), Postives = 371/652 (56.90%), Query Frame = 0

Query: 6   ASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQML-TNSIHK 65
           A P  + R+ NF    SNPN  P       + +  +++C++++QLKQ H  M+ T +   
Sbjct: 7   AQPLSLPRHPNF----SNPN-QPTTNNERSRHISLIERCVSLRQLKQTHGHMIRTGTFSD 66

Query: 66  PNSF--LYKIVDLKDFA---YASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSR 125
           P S   L+ +  L  FA   YA   F +I +P  +++N +IR  ++  +       F   
Sbjct: 67  PYSASKLFAMAALSSFASLEYARKVFDEIPKPNSFAWNTLIRAYASGPDPVLSIWAFLDM 126

Query: 126 MKFLGLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGD 185
           +      PNK T+PFL  A + + ++  G+  H   +K  +  D  V++SLI  Y  CGD
Sbjct: 127 VSESQCYPNKYTFPFLIKAAAEVSSLSLGQSLHGMAVKSAVGSDVFVANSLIHCYFSCGD 186

Query: 186 LGDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGA 245
           L  A KVF  I E+D+VSWNSMI+G+ +     +A+ LF++M     + + +T+V VL A
Sbjct: 187 LDSACKVFTTIKEKDVVSWNSMINGFVQKGSPDKALELFKKMESEDVKASHVTMVGVLSA 246

Query: 246 CGELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVT- 305
           C ++ +L+ G  V  ++ EN++ +N  + +A++ MY KCG +  A+R+FD+M +KD VT 
Sbjct: 247 CAKIRNLEFGRQVCSYIEENRVNVNLTLANAMLDMYTKCGSIEDAKRLFDAMEEKDNVTW 306

Query: 306 ------------------------------WNAMITGYAQNGMSEEAIKLFQDMRMSSTT 365
                                         WNA+I+ Y QNG   EA+ +F ++++    
Sbjct: 307 TTMLDGYAISEDYEAAREVLNSMPQKDIVAWNALISAYEQNGKPNEALIVFHELQLQKNM 366

Query: 366 P-DQITLIGMLSACASIGALDLGKQVEIYASERGLRDDIYVATALVDMYAKCGSLDNAFR 425
             +QITL+  LSACA +GAL+LG+ +  Y  + G+R + +V +AL+ MY+KCG L+ +  
Sbjct: 367 KLNQITLVSTLSACAQVGALELGRWIHSYIKKHGIRMNFHVTSALIHMYSKCGDLEKSRE 426

Query: 426 VFDGMPRKNEVSWNAMISALAFHGQAQEALALFKSMIKEGGAASPNDITFVGVLSACVHA 485
           VF+ + +++   W+AMI  LA HG   EA+ +F  M  +     PN +TF  V  AC H 
Sbjct: 427 VFNSVEKRDVFVWSAMIGGLAMHGCGNEAVDMFYKM--QEANVKPNGVTFTNVFCACSHT 486

Query: 486 GLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLLSRAGHLEEAWDFVEKMPEKPDEVILGA 545
           GLVDE   LFH M S++G+VP+ +HY+C+VD+L R+G+LE+A  F+E MP  P   + GA
Sbjct: 487 GLVDEAESLFHQMESNYGIVPEEKHYACIVDVLGRSGYLEKAVKFIEAMPIPPSTSVWGA 546

Query: 546 LLGACQKRKNVDISERVMKLLLELEPSNSGNYVISSRLYANLRRWDDSARMRMMMKQKGV 605
           LLGAC+   N++++E     LLELEP N G +V+ S +YA L +W++ + +R  M+  G+
Sbjct: 547 LLGACKIHANLNLAEMACTRLLELEPRNDGAHVLLSNIYAKLGKWENVSELRKHMRVTGL 606

Query: 606 SKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQILELLIDDLRREGYIPNTS 620
            K PGCS I+ID  +HEF +GD  H    +++  L  +++ L+  GY P  S
Sbjct: 607 KKEPGCSSIEIDGMIHEFLSGDNAHPMSEKVYGKLHEVMEKLKSNGYEPEIS 651

BLAST of Tan0009250 vs. ExPASy Swiss-Prot
Match: Q9SJZ3 (Pentatricopeptide repeat-containing protein At2g22410, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-E28 PE=2 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 4.5e-120
Identity = 249/646 (38.54%), Postives = 373/646 (57.74%), Query Frame = 0

Query: 3   RIKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTNSI 62
           R ++ PH   + +N++ THS    NP        L+  L++C  +  LKQI  QM+ N +
Sbjct: 30  RTRSLPHHRDKPINWNSTHSFVLHNP--------LLSLLEKCKLLLHLKQIQAQMIINGL 89

Query: 63  ---HKPNSFLYKIVDLKDFAYASDFFSKILE----PTEYSFNVMIRGLSTTWNKSSLALE 122
                 +S L     L +  Y  D+  KIL+    P  +S+NV IRG S + N     L 
Sbjct: 90  ILDPFASSRLIAFCALSESRYL-DYSVKILKGIENPNIFSWNVTIRGFSESENPKESFL- 149

Query: 123 FYSRMKFLGL---KPNKLTYPFLFIACSN--LLAVGHGRVGHSSVIKRGLDEDGHVSHSL 182
            Y +M   G    +P+  TYP LF  C++  L ++GH  +GH  V+K  L+   HV ++ 
Sbjct: 150 LYKQMLRHGCCESRPDHFTYPVLFKVCADLRLSSLGHMILGH--VLKLRLELVSHVHNAS 209

Query: 183 ITMYARCGDLGDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNE 242
           I M+A CGD+ +ARKVFDE P RDLVSWN +I+GY K+  A +A+ +++ M   G +P++
Sbjct: 210 IHMFASCGDMENARKVFDESPVRDLVSWNCLINGYKKIGEAEKAIYVYKLMESEGVKPDD 269

Query: 243 MTLVSVLGACGELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDS 302
           +T++ ++ +C  LGDL  G    E+V EN + +   + +AL+ M+ KCGD+  ARRIFD+
Sbjct: 270 VTMIGLVSSCSMLGDLNRGKEFYEYVKENGLRMTIPLVNALMDMFSKCGDIHEARRIFDN 329

Query: 303 MRKKDKVTWNAMITGYAQNGM-------------------------------SEEAIKLF 362
           + K+  V+W  MI+GYA+ G+                                ++A+ LF
Sbjct: 330 LEKRTIVSWTTMISGYARCGLLDVSRKLFDDMEEKDVVLWNAMIGGSVQAKRGQDALALF 389

Query: 363 QDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGLRDDIYVATALVDMYAKC 422
           Q+M+ S+T PD+IT+I  LSAC+ +GALD+G  +  Y  +  L  ++ + T+LVDMYAKC
Sbjct: 390 QEMQTSNTKPDEITMIHCLSACSQLGALDVGIWIHRYIEKYSLSLNVALGTSLVDMYAKC 449

Query: 423 GSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALFKSMIKEGGAASPNDITFVG 482
           G++  A  VF G+  +N +++ A+I  LA HG A  A++ F  MI  G   +P++ITF+G
Sbjct: 450 GNISEALSVFHGIQTRNSLTYTAIIGGLALHGDASTAISYFNEMIDAG--IAPDEITFIG 509

Query: 483 VLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLLSRAGHLEEAWDFVEKMPEK 542
           +LSAC H G++  GR  F  M S F L P+++HYS MVDLL RAG LEEA   +E MP +
Sbjct: 510 LLSACCHGGMIQTGRDYFSQMKSRFNLNPQLKHYSIMVDLLGRAGLLEEADRLMESMPME 569

Query: 543 PDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSGNYVISSRLYANLRRWDDSARMR 602
            D  + GALL  C+   NV++ E+  K LLEL+PS+SG YV+   +Y     W+D+ R R
Sbjct: 570 ADAAVWGALLFGCRMHGNVELGEKAAKKLLELDPSDSGIYVLLDGMYGEANMWEDAKRAR 629

Query: 603 MMMKQKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQILELL 606
            MM ++GV K PGCS I+++  + EF   D    E  +I+  L  L
Sbjct: 630 RMMNERGVEKIPGCSSIEVNGIVCEFIVRDKSRPESEKIYDRLHCL 661

BLAST of Tan0009250 vs. ExPASy Swiss-Prot
Match: Q9SR82 (Putative pentatricopeptide repeat-containing protein At3g08820 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H84 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 1.5e-118
Identity = 225/598 (37.63%), Postives = 352/598 (58.86%), Query Frame = 0

Query: 28  PNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTNSIHKP----NSFLYKIVDLKDFAYASD 87
           P+  +  Q++   +    T+  LKQIH  ++ + +H      N  L + +  +   Y+  
Sbjct: 7   PSATSKVQQIKTLISVACTVNHLKQIHVSLINHHLHHDTFLVNLLLKRTLFFRQTKYSYL 66

Query: 88  FFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSRMKFLGLKPNKLTYPFLFIACSNLL 147
            FS    P  + +N +I G     +     L+ +  ++  GL  +  T+P +  AC+   
Sbjct: 67  LFSHTQFPNIFLYNSLINGFVNN-HLFHETLDLFLSIRKHGLYLHGFTFPLVLKACTRAS 126

Query: 148 AVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGDLGDARKVFDEIPERDLVSWNSMIS 207
           +   G   HS V+K G + D     SL+++Y+  G L DA K+FDEIP+R +V+W ++ S
Sbjct: 127 SRKLGIDLHSLVVKCGFNHDVAAMTSLLSIYSGSGRLNDAHKLFDEIPDRSVVTWTALFS 186

Query: 208 GYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGELGDLKLGTWVEEFVVENKMVL 267
           GY+      EA+ LF++M+E G +P+   +V VL AC  +GDL  G W+ +++ E +M  
Sbjct: 187 GYTTSGRHREAIDLFKKMVEMGVKPDSYFIVQVLSACVHVGDLDSGEWIVKYMEEMEMQK 246

Query: 268 NSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTWNAMITGYAQNGMSEEAIKLFQDMR 327
           NSF+ + L+++Y KCG +  AR +FDSM +KD VTW+ MI GYA N   +E I+LF  M 
Sbjct: 247 NSFVRTTLVNLYAKCGKMEKARSVFDSMVEKDIVTWSTMIQGYASNSFPKEGIELFLQML 306

Query: 328 MSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGLRDDIYVATALVDMYAKCGSLD 387
             +  PDQ +++G LS+CAS+GALDLG+             ++++A AL+DMYAKCG++ 
Sbjct: 307 QENLKPDQFSIVGFLSSCASLGALDLGEWGISLIDRHEFLTNLFMANALIDMYAKCGAMA 366

Query: 388 NAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALFKSMIKEGGAASPNDITFVGVLSA 447
             F VF  M  K+ V  NA IS LA +G  + + A+F    K G   SP+  TF+G+L  
Sbjct: 367 RGFEVFKEMKEKDIVIMNAAISGLAKNGHVKLSFAVFGQTEKLG--ISPDGSTFLGLLCG 426

Query: 448 CVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLLSRAGHLEEAWDFVEKMPEKPDEV 507
           CVHAGL+ +G R F+ +S  + L   +EHY CMVDL  RAG L++A+  +  MP +P+ +
Sbjct: 427 CVHAGLIQDGLRFFNAISCVYALKRTVEHYGCMVDLWGRAGMLDDAYRLICDMPMRPNAI 486

Query: 508 ILGALLGACQKRKNVDISERVMKLLLELEPSNSGNYVISSRLYANLRRWDDSARMRMMMK 567
           + GALL  C+  K+  ++E V+K L+ LEP N+GNYV  S +Y+   RWD++A +R MM 
Sbjct: 487 VWGALLSGCRLVKDTQLAETVLKELIALEPWNAGNYVQLSNIYSVGGRWDEAAEVRDMMN 546

Query: 568 QKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQILELLIDDLRREGYIPNTSLL 622
           +KG+ K PG SWI+++ ++HEF A D  H    +I+  LE L +++R  G++P T  +
Sbjct: 547 KKGMKKIPGYSWIELEGKVHEFLADDKSHPLSDKIYAKLEDLGNEMRLMGFVPTTEFV 601

BLAST of Tan0009250 vs. NCBI nr
Match: XP_022929625.1 (pentatricopeptide repeat-containing protein At2g34400 [Cucurbita moschata])

HSP 1 Score: 1173.3 bits (3034), Expect = 0.0e+00
Identity = 577/621 (92.91%), Postives = 608/621 (97.91%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTN 60
           MLRIK+SPHLISRYLNFDKTHS   PNPNRQA S+KLV FLQQC++IKQLKQIH QMLTN
Sbjct: 1   MLRIKSSPHLISRYLNFDKTHS--KPNPNRQAFSEKLVTFLQQCVSIKQLKQIHNQMLTN 60

Query: 61  SIHKPNSFLYKIVDLKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSRM 120
           SIHKPNSFLYK+VDLKDFAYASDFFSKILEPTEY+FNVMIRGLSTTWNKSSLALEFYSRM
Sbjct: 61  SIHKPNSFLYKVVDLKDFAYASDFFSKILEPTEYAFNVMIRGLSTTWNKSSLALEFYSRM 120

Query: 121 KFLGLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGDL 180
           KFLGLKPNKLTYPFLFIACSNLLAV +GRVGHSSVIKRGLDEDGHVSHSLITMYARCG++
Sbjct: 121 KFLGLKPNKLTYPFLFIACSNLLAVENGRVGHSSVIKRGLDEDGHVSHSLITMYARCGEV 180

Query: 181 GDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGAC 240
           GDARKVFDEIPE+DLVSWNSMISGYSKMR+A EAVGLFR+MM+AGF+PNEMTLVSVLGAC
Sbjct: 181 GDARKVFDEIPEKDLVSWNSMISGYSKMRHAVEAVGLFRDMMQAGFDPNEMTLVSVLGAC 240

Query: 241 GELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTWN 300
           GELGDLKLGTWVEEFVV+N+MVLNS+MGSALIHMYGKCGDLVSARRIFDSM+KKDKVTWN
Sbjct: 241 GELGDLKLGTWVEEFVVDNRMVLNSYMGSALIHMYGKCGDLVSARRIFDSMKKKDKVTWN 300

Query: 301 AMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASER 360
           AMITGYAQNGMSEEAIKLFQDMR+SST PDQITL+G LSACASIGALDLGKQVEIYASER
Sbjct: 301 AMITGYAQNGMSEEAIKLFQDMRISSTAPDQITLVGTLSACASIGALDLGKQVEIYASER 360

Query: 361 GLRDDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALF 420
           GLRD+IYV+TALVDMYAKCGSLDNAFRVFD MP+KNEVSWNAMISALAFHGQAQ+ALALF
Sbjct: 361 GLRDNIYVSTALVDMYAKCGSLDNAFRVFDVMPQKNEVSWNAMISALAFHGQAQKALALF 420

Query: 421 KSMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLL 480
           +SMIKEGGAASPNDITFVGVLSACVHAGLVDEGRR FHMMSSSFGLVPKIEHYSCMVDLL
Sbjct: 421 ESMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRFFHMMSSSFGLVPKIEHYSCMVDLL 480

Query: 481 SRAGHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSGNYV 540
           SRAGHLEEAWDFV+KMPEKPDEVILGALLGACQKRKNV+ISERVMKLLLELEPSNSGNYV
Sbjct: 481 SRAGHLEEAWDFVKKMPEKPDEVILGALLGACQKRKNVEISERVMKLLLELEPSNSGNYV 540

Query: 541 ISSRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQ 600
           ISSRLYANLRRWDDSARMRM+MKQKGVSKTPGCSWIDI+SQLHEFHAGDVLHQEWIEI Q
Sbjct: 541 ISSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIQQ 600

Query: 601 ILELLIDDLRREGYIPNTSLL 622
           IL+LLIDDLRREGYIPNT+LL
Sbjct: 601 ILDLLIDDLRREGYIPNTTLL 619

BLAST of Tan0009250 vs. NCBI nr
Match: XP_023521362.1 (pentatricopeptide repeat-containing protein At2g34400-like [Cucurbita pepo subsp. pepo] >XP_023545668.1 pentatricopeptide repeat-containing protein At2g34400-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 576/621 (92.75%), Postives = 607/621 (97.75%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTN 60
           MLRIK+SPHLISRYLNFDKTHS   PNPNRQALS+KLV FLQQC++IKQLKQIH QMLTN
Sbjct: 1   MLRIKSSPHLISRYLNFDKTHS--KPNPNRQALSEKLVTFLQQCVSIKQLKQIHNQMLTN 60

Query: 61  SIHKPNSFLYKIVDLKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSRM 120
           SIHKPNSFLYK+VDLKDFAYASDFFSKILEPTEY+FNVMIRGLSTTWNKSSLALEFYSRM
Sbjct: 61  SIHKPNSFLYKVVDLKDFAYASDFFSKILEPTEYTFNVMIRGLSTTWNKSSLALEFYSRM 120

Query: 121 KFLGLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGDL 180
           KFLGLKPNKLTYPFLFIACSNLLAV +GRVGHSSVIKRGLDEDGHVSHSLITMYARCG++
Sbjct: 121 KFLGLKPNKLTYPFLFIACSNLLAVENGRVGHSSVIKRGLDEDGHVSHSLITMYARCGEV 180

Query: 181 GDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGAC 240
           GDARKVFDEIPE+DLVSWNSMISGYSKMR+A EAVGLFR+MM+AGF+PNEMTLVSVLGAC
Sbjct: 181 GDARKVFDEIPEKDLVSWNSMISGYSKMRHAVEAVGLFRDMMQAGFDPNEMTLVSVLGAC 240

Query: 241 GELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTWN 300
           GELGDLKLGTWVEEFVV+N+MVLNS+MGSALIHMYGKCGDLVSARRIFDSM+KKDKVTWN
Sbjct: 241 GELGDLKLGTWVEEFVVDNRMVLNSYMGSALIHMYGKCGDLVSARRIFDSMKKKDKVTWN 300

Query: 301 AMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASER 360
           AMITGYAQNGMSEEAIKLFQDMR+SST PDQITL+G LSACASIGALDLGKQVEIYASER
Sbjct: 301 AMITGYAQNGMSEEAIKLFQDMRISSTAPDQITLVGTLSACASIGALDLGKQVEIYASER 360

Query: 361 GLRDDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALF 420
           GL+D+IYV+TALVDMYAKCGSLDNAFRVFD MPRKNEVSWNAMISALAFHGQAQ+ALALF
Sbjct: 361 GLQDNIYVSTALVDMYAKCGSLDNAFRVFDIMPRKNEVSWNAMISALAFHGQAQKALALF 420

Query: 421 KSMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLL 480
           KSMI EGG ASPNDITFVGVLSACVHAGLVDEGRR FHMMSSSFGLVPKIEHYSCMVDLL
Sbjct: 421 KSMINEGGDASPNDITFVGVLSACVHAGLVDEGRRFFHMMSSSFGLVPKIEHYSCMVDLL 480

Query: 481 SRAGHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSGNYV 540
           SRAGHLEEAWDFV+KMPEKPDEVILGALLGACQKRKNV+ISERVM+LLLELEPSNSGNYV
Sbjct: 481 SRAGHLEEAWDFVKKMPEKPDEVILGALLGACQKRKNVEISERVMELLLELEPSNSGNYV 540

Query: 541 ISSRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQ 600
           ISSRLYANLRRWDDSARMRM+MKQKGVSKTPGCSWIDI+SQLHEFHAGDVLHQEWIEI Q
Sbjct: 541 ISSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIQQ 600

Query: 601 ILELLIDDLRREGYIPNTSLL 622
           IL+LLIDDLRREGYIPNT+LL
Sbjct: 601 ILDLLIDDLRREGYIPNTTLL 619

BLAST of Tan0009250 vs. NCBI nr
Match: XP_022153282.1 (pentatricopeptide repeat-containing protein At2g34400 [Momordica charantia])

HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 581/623 (93.26%), Postives = 603/623 (96.79%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGF-LQQCLTIKQLKQIHTQMLT 60
           MLRIKASPHLISRYLN +KT    NPNPNR+ALS+KL+ F LQQCL+++QLKQIHTQM+T
Sbjct: 1   MLRIKASPHLISRYLNLEKT----NPNPNRRALSEKLLAFLLQQCLSLRQLKQIHTQMIT 60

Query: 61  NSIHKPNSFLYKIVDLKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSR 120
           NSIHKPNSFL+KI DLKDFAYASDFFS+ILEPTEY+FNVMIRGLSTTWNKSSLALE Y R
Sbjct: 61  NSIHKPNSFLFKIADLKDFAYASDFFSRILEPTEYAFNVMIRGLSTTWNKSSLALELYFR 120

Query: 121 MKFLGLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGD 180
           MKFLGLKPNKLTYPFLFIACSNLLAV +GRVGHSSV+KRG DEDGHVSHSLITMYARCG+
Sbjct: 121 MKFLGLKPNKLTYPFLFIACSNLLAVEYGRVGHSSVVKRGFDEDGHVSHSLITMYARCGE 180

Query: 181 LGDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGA 240
           LGDARKVFDEIPERDLVSWNSMISGYSKMRYA EAVGLFREMM AGFEPNEMTLVSVLGA
Sbjct: 181 LGDARKVFDEIPERDLVSWNSMISGYSKMRYAAEAVGLFREMMGAGFEPNEMTLVSVLGA 240

Query: 241 CGELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTW 300
           CGELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIF+SM+KKDKVTW
Sbjct: 241 CGELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFESMKKKDKVTW 300

Query: 301 NAMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASE 360
           NAMITGYAQNG+SEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQ EIYASE
Sbjct: 301 NAMITGYAQNGLSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQAEIYASE 360

Query: 361 RGLRDDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALAL 420
           RGLRDDIYVATALVDMYAKCGSLDNAFRVF+GMPRKNEVSWNAMISALAFHGQAQEALAL
Sbjct: 361 RGLRDDIYVATALVDMYAKCGSLDNAFRVFNGMPRKNEVSWNAMISALAFHGQAQEALAL 420

Query: 421 FKSMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDL 480
           FKSMIKEGG+ASPNDITFVGVLSACVHAGLVDEGRRLFHMM SSF LVPKIEHYSCMVDL
Sbjct: 421 FKSMIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMCSSFRLVPKIEHYSCMVDL 480

Query: 481 LSRAGHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSGNY 540
           LSRAGHLEEAWDF+EKMPEKPDEVILGALLGACQKRKN+DISERVMKLLLELEPSNSGNY
Sbjct: 481 LSRAGHLEEAWDFIEKMPEKPDEVILGALLGACQKRKNIDISERVMKLLLELEPSNSGNY 540

Query: 541 VISSRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHA-GDVLHQEWIEI 600
           VISSRLYANLRRWDDSARMRM+MKQKGVSKTPGCSWIDIDSQLHEFHA GDVLH EWIEI
Sbjct: 541 VISSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDIDSQLHEFHAGGDVLHHEWIEI 600

Query: 601 HQILELLIDDLRREGYIPNTSLL 622
           HQIL LLIDDLRREGYIPNTSLL
Sbjct: 601 HQILNLLIDDLRREGYIPNTSLL 619

BLAST of Tan0009250 vs. NCBI nr
Match: XP_022997564.1 (pentatricopeptide repeat-containing protein At2g34400 [Cucurbita maxima])

HSP 1 Score: 1169.5 bits (3024), Expect = 0.0e+00
Identity = 574/621 (92.43%), Postives = 606/621 (97.58%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTN 60
           MLRIK+SPHLISRYLNFDKTHS   PNPNRQALS+KLV FLQQCL+IKQ+KQIH QMLTN
Sbjct: 1   MLRIKSSPHLISRYLNFDKTHS--KPNPNRQALSEKLVTFLQQCLSIKQMKQIHNQMLTN 60

Query: 61  SIHKPNSFLYKIVDLKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSRM 120
           SIHKPNSFLYK+VDLKDFAYASDFFSKILEPTEY+FNVMIRGLSTTWNKSSLALEFYSRM
Sbjct: 61  SIHKPNSFLYKVVDLKDFAYASDFFSKILEPTEYAFNVMIRGLSTTWNKSSLALEFYSRM 120

Query: 121 KFLGLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGDL 180
           KFLGLKPNKLTYPFLFIACSNLLAV +GRVGHSSVIKRGLDEDGHVSHSLITMYARCG++
Sbjct: 121 KFLGLKPNKLTYPFLFIACSNLLAVENGRVGHSSVIKRGLDEDGHVSHSLITMYARCGEV 180

Query: 181 GDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGAC 240
           GDARKVFDEIPE+DLVSWNSMISGYSKMR+A EAVGLF +MM+AGFEPNEMTLVSVLGAC
Sbjct: 181 GDARKVFDEIPEKDLVSWNSMISGYSKMRHAVEAVGLFSDMMQAGFEPNEMTLVSVLGAC 240

Query: 241 GELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTWN 300
           GELGDLKLGTWVEEFV++N+MVLNS+MGSALIHMYGKCGDLVSARRIFDSM+KKDKVTWN
Sbjct: 241 GELGDLKLGTWVEEFVMDNRMVLNSYMGSALIHMYGKCGDLVSARRIFDSMKKKDKVTWN 300

Query: 301 AMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASER 360
           AMITGYAQNGMSEEAIKLFQDMR+SST PDQITL+G LSACASIGALDLGKQVEIYASER
Sbjct: 301 AMITGYAQNGMSEEAIKLFQDMRISSTAPDQITLVGTLSACASIGALDLGKQVEIYASER 360

Query: 361 GLRDDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALF 420
           GLRD+IYV+TALVDMY+KCGSLDNAFRVFD MPRKNEVSWNAMISALAFHGQAQ+A+ALF
Sbjct: 361 GLRDNIYVSTALVDMYSKCGSLDNAFRVFDVMPRKNEVSWNAMISALAFHGQAQKAMALF 420

Query: 421 KSMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLL 480
            SM+ EGGAASPNDITFVGVLSACVHAGLVDEGRR FHMMSSSFGLVPKIEHYSCMVDLL
Sbjct: 421 NSMLNEGGAASPNDITFVGVLSACVHAGLVDEGRRFFHMMSSSFGLVPKIEHYSCMVDLL 480

Query: 481 SRAGHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSGNYV 540
           SRAGHLEEAWDFV+KMPEKPDEVILGALLGACQKRKNV+ISERVMKLLLELEPSNSGNYV
Sbjct: 481 SRAGHLEEAWDFVKKMPEKPDEVILGALLGACQKRKNVEISERVMKLLLELEPSNSGNYV 540

Query: 541 ISSRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQ 600
           ISSRLYANLRRWDDSARMRM+MKQKGVSKTPGCSWIDI+SQLHEFHAGDVLHQEWIEI Q
Sbjct: 541 ISSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIQQ 600

Query: 601 ILELLIDDLRREGYIPNTSLL 622
           IL+LLIDDLRREGYIPNT+LL
Sbjct: 601 ILDLLIDDLRREGYIPNTTLL 619

BLAST of Tan0009250 vs. NCBI nr
Match: XP_038889591.1 (pentatricopeptide repeat-containing protein At2g34400 [Benincasa hispida])

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 579/621 (93.24%), Postives = 601/621 (96.78%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTN 60
           MLRIKASPHLISRY NF+KT  N  P+PNRQALS+KL  FLQQC++IKQLKQIHTQMLTN
Sbjct: 1   MLRIKASPHLISRYFNFNKT--NSKPSPNRQALSEKLNSFLQQCVSIKQLKQIHTQMLTN 60

Query: 61  SIHKPNSFLYKIVDLKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSRM 120
           SIHKPNSFLYKIVDLKDFAY+ DFFSKI+EPTEYSFNVMIRGL+ TWNKSSLALE Y RM
Sbjct: 61  SIHKPNSFLYKIVDLKDFAYSLDFFSKIVEPTEYSFNVMIRGLAKTWNKSSLALELYYRM 120

Query: 121 KFLGLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGDL 180
           KFLGLKPNKLTYPFLFIACSNLLAV +GRVGH SVIKRGLDEDGHVSHSLITMYARCG +
Sbjct: 121 KFLGLKPNKLTYPFLFIACSNLLAVENGRVGHCSVIKRGLDEDGHVSHSLITMYARCGQV 180

Query: 181 GDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGAC 240
           GDARKVFDEIPE+DLVSW+SMISGYSKMR+AGEAVGLFREMMEAGF+PNEM LVSVLGAC
Sbjct: 181 GDARKVFDEIPEKDLVSWSSMISGYSKMRHAGEAVGLFREMMEAGFQPNEMALVSVLGAC 240

Query: 241 GELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTWN 300
           GELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSM+KKDKVTWN
Sbjct: 241 GELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMKKKDKVTWN 300

Query: 301 AMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASER 360
           AMITGYAQNGMS+EAIKLFQDMRMSST PDQITLIG+LSACASIGALDLGKQVEIYASER
Sbjct: 301 AMITGYAQNGMSDEAIKLFQDMRMSSTAPDQITLIGILSACASIGALDLGKQVEIYASER 360

Query: 361 GLRDDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALF 420
           GLRDDIYVATALVDMYAKCGSLDNAFRVF GMPRKNEVSWNAMISALAFHGQAQEALALF
Sbjct: 361 GLRDDIYVATALVDMYAKCGSLDNAFRVFYGMPRKNEVSWNAMISALAFHGQAQEALALF 420

Query: 421 KSMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLL 480
           KSMI EGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLL
Sbjct: 421 KSMINEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLL 480

Query: 481 SRAGHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSGNYV 540
           SRAGHLEEAWDFV+KMPEKPDEVILGALLGACQKRKN+DISERVMKLLLELEPSNSGNYV
Sbjct: 481 SRAGHLEEAWDFVKKMPEKPDEVILGALLGACQKRKNIDISERVMKLLLELEPSNSGNYV 540

Query: 541 ISSRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQ 600
           ISSRLYANLRRWDDSARMRM+MKQKGVSKTPGCSWIDI+SQLHEFHAGDVLHQEWIEI Q
Sbjct: 541 ISSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIRQ 600

Query: 601 ILELLIDDLRREGYIPNTSLL 622
           IL+LLIDDLRREGYIPN SLL
Sbjct: 601 ILDLLIDDLRREGYIPNISLL 619

BLAST of Tan0009250 vs. ExPASy TrEMBL
Match: A0A6J1EUW3 (pentatricopeptide repeat-containing protein At2g34400 OS=Cucurbita moschata OX=3662 GN=LOC111436152 PE=4 SV=1)

HSP 1 Score: 1173.3 bits (3034), Expect = 0.0e+00
Identity = 577/621 (92.91%), Postives = 608/621 (97.91%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTN 60
           MLRIK+SPHLISRYLNFDKTHS   PNPNRQA S+KLV FLQQC++IKQLKQIH QMLTN
Sbjct: 1   MLRIKSSPHLISRYLNFDKTHS--KPNPNRQAFSEKLVTFLQQCVSIKQLKQIHNQMLTN 60

Query: 61  SIHKPNSFLYKIVDLKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSRM 120
           SIHKPNSFLYK+VDLKDFAYASDFFSKILEPTEY+FNVMIRGLSTTWNKSSLALEFYSRM
Sbjct: 61  SIHKPNSFLYKVVDLKDFAYASDFFSKILEPTEYAFNVMIRGLSTTWNKSSLALEFYSRM 120

Query: 121 KFLGLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGDL 180
           KFLGLKPNKLTYPFLFIACSNLLAV +GRVGHSSVIKRGLDEDGHVSHSLITMYARCG++
Sbjct: 121 KFLGLKPNKLTYPFLFIACSNLLAVENGRVGHSSVIKRGLDEDGHVSHSLITMYARCGEV 180

Query: 181 GDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGAC 240
           GDARKVFDEIPE+DLVSWNSMISGYSKMR+A EAVGLFR+MM+AGF+PNEMTLVSVLGAC
Sbjct: 181 GDARKVFDEIPEKDLVSWNSMISGYSKMRHAVEAVGLFRDMMQAGFDPNEMTLVSVLGAC 240

Query: 241 GELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTWN 300
           GELGDLKLGTWVEEFVV+N+MVLNS+MGSALIHMYGKCGDLVSARRIFDSM+KKDKVTWN
Sbjct: 241 GELGDLKLGTWVEEFVVDNRMVLNSYMGSALIHMYGKCGDLVSARRIFDSMKKKDKVTWN 300

Query: 301 AMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASER 360
           AMITGYAQNGMSEEAIKLFQDMR+SST PDQITL+G LSACASIGALDLGKQVEIYASER
Sbjct: 301 AMITGYAQNGMSEEAIKLFQDMRISSTAPDQITLVGTLSACASIGALDLGKQVEIYASER 360

Query: 361 GLRDDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALF 420
           GLRD+IYV+TALVDMYAKCGSLDNAFRVFD MP+KNEVSWNAMISALAFHGQAQ+ALALF
Sbjct: 361 GLRDNIYVSTALVDMYAKCGSLDNAFRVFDVMPQKNEVSWNAMISALAFHGQAQKALALF 420

Query: 421 KSMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLL 480
           +SMIKEGGAASPNDITFVGVLSACVHAGLVDEGRR FHMMSSSFGLVPKIEHYSCMVDLL
Sbjct: 421 ESMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRFFHMMSSSFGLVPKIEHYSCMVDLL 480

Query: 481 SRAGHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSGNYV 540
           SRAGHLEEAWDFV+KMPEKPDEVILGALLGACQKRKNV+ISERVMKLLLELEPSNSGNYV
Sbjct: 481 SRAGHLEEAWDFVKKMPEKPDEVILGALLGACQKRKNVEISERVMKLLLELEPSNSGNYV 540

Query: 541 ISSRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQ 600
           ISSRLYANLRRWDDSARMRM+MKQKGVSKTPGCSWIDI+SQLHEFHAGDVLHQEWIEI Q
Sbjct: 541 ISSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIQQ 600

Query: 601 ILELLIDDLRREGYIPNTSLL 622
           IL+LLIDDLRREGYIPNT+LL
Sbjct: 601 ILDLLIDDLRREGYIPNTTLL 619

BLAST of Tan0009250 vs. ExPASy TrEMBL
Match: A0A6J1DIG6 (pentatricopeptide repeat-containing protein At2g34400 OS=Momordica charantia OX=3673 GN=LOC111020815 PE=4 SV=1)

HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 581/623 (93.26%), Postives = 603/623 (96.79%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGF-LQQCLTIKQLKQIHTQMLT 60
           MLRIKASPHLISRYLN +KT    NPNPNR+ALS+KL+ F LQQCL+++QLKQIHTQM+T
Sbjct: 1   MLRIKASPHLISRYLNLEKT----NPNPNRRALSEKLLAFLLQQCLSLRQLKQIHTQMIT 60

Query: 61  NSIHKPNSFLYKIVDLKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSR 120
           NSIHKPNSFL+KI DLKDFAYASDFFS+ILEPTEY+FNVMIRGLSTTWNKSSLALE Y R
Sbjct: 61  NSIHKPNSFLFKIADLKDFAYASDFFSRILEPTEYAFNVMIRGLSTTWNKSSLALELYFR 120

Query: 121 MKFLGLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGD 180
           MKFLGLKPNKLTYPFLFIACSNLLAV +GRVGHSSV+KRG DEDGHVSHSLITMYARCG+
Sbjct: 121 MKFLGLKPNKLTYPFLFIACSNLLAVEYGRVGHSSVVKRGFDEDGHVSHSLITMYARCGE 180

Query: 181 LGDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGA 240
           LGDARKVFDEIPERDLVSWNSMISGYSKMRYA EAVGLFREMM AGFEPNEMTLVSVLGA
Sbjct: 181 LGDARKVFDEIPERDLVSWNSMISGYSKMRYAAEAVGLFREMMGAGFEPNEMTLVSVLGA 240

Query: 241 CGELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTW 300
           CGELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIF+SM+KKDKVTW
Sbjct: 241 CGELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFESMKKKDKVTW 300

Query: 301 NAMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASE 360
           NAMITGYAQNG+SEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQ EIYASE
Sbjct: 301 NAMITGYAQNGLSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQAEIYASE 360

Query: 361 RGLRDDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALAL 420
           RGLRDDIYVATALVDMYAKCGSLDNAFRVF+GMPRKNEVSWNAMISALAFHGQAQEALAL
Sbjct: 361 RGLRDDIYVATALVDMYAKCGSLDNAFRVFNGMPRKNEVSWNAMISALAFHGQAQEALAL 420

Query: 421 FKSMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDL 480
           FKSMIKEGG+ASPNDITFVGVLSACVHAGLVDEGRRLFHMM SSF LVPKIEHYSCMVDL
Sbjct: 421 FKSMIKEGGSASPNDITFVGVLSACVHAGLVDEGRRLFHMMCSSFRLVPKIEHYSCMVDL 480

Query: 481 LSRAGHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSGNY 540
           LSRAGHLEEAWDF+EKMPEKPDEVILGALLGACQKRKN+DISERVMKLLLELEPSNSGNY
Sbjct: 481 LSRAGHLEEAWDFIEKMPEKPDEVILGALLGACQKRKNIDISERVMKLLLELEPSNSGNY 540

Query: 541 VISSRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHA-GDVLHQEWIEI 600
           VISSRLYANLRRWDDSARMRM+MKQKGVSKTPGCSWIDIDSQLHEFHA GDVLH EWIEI
Sbjct: 541 VISSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDIDSQLHEFHAGGDVLHHEWIEI 600

Query: 601 HQILELLIDDLRREGYIPNTSLL 622
           HQIL LLIDDLRREGYIPNTSLL
Sbjct: 601 HQILNLLIDDLRREGYIPNTSLL 619

BLAST of Tan0009250 vs. ExPASy TrEMBL
Match: A0A6J1KBU4 (pentatricopeptide repeat-containing protein At2g34400 OS=Cucurbita maxima OX=3661 GN=LOC111492454 PE=4 SV=1)

HSP 1 Score: 1169.5 bits (3024), Expect = 0.0e+00
Identity = 574/621 (92.43%), Postives = 606/621 (97.58%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTN 60
           MLRIK+SPHLISRYLNFDKTHS   PNPNRQALS+KLV FLQQCL+IKQ+KQIH QMLTN
Sbjct: 1   MLRIKSSPHLISRYLNFDKTHS--KPNPNRQALSEKLVTFLQQCLSIKQMKQIHNQMLTN 60

Query: 61  SIHKPNSFLYKIVDLKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSRM 120
           SIHKPNSFLYK+VDLKDFAYASDFFSKILEPTEY+FNVMIRGLSTTWNKSSLALEFYSRM
Sbjct: 61  SIHKPNSFLYKVVDLKDFAYASDFFSKILEPTEYAFNVMIRGLSTTWNKSSLALEFYSRM 120

Query: 121 KFLGLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGDL 180
           KFLGLKPNKLTYPFLFIACSNLLAV +GRVGHSSVIKRGLDEDGHVSHSLITMYARCG++
Sbjct: 121 KFLGLKPNKLTYPFLFIACSNLLAVENGRVGHSSVIKRGLDEDGHVSHSLITMYARCGEV 180

Query: 181 GDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGAC 240
           GDARKVFDEIPE+DLVSWNSMISGYSKMR+A EAVGLF +MM+AGFEPNEMTLVSVLGAC
Sbjct: 181 GDARKVFDEIPEKDLVSWNSMISGYSKMRHAVEAVGLFSDMMQAGFEPNEMTLVSVLGAC 240

Query: 241 GELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTWN 300
           GELGDLKLGTWVEEFV++N+MVLNS+MGSALIHMYGKCGDLVSARRIFDSM+KKDKVTWN
Sbjct: 241 GELGDLKLGTWVEEFVMDNRMVLNSYMGSALIHMYGKCGDLVSARRIFDSMKKKDKVTWN 300

Query: 301 AMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASER 360
           AMITGYAQNGMSEEAIKLFQDMR+SST PDQITL+G LSACASIGALDLGKQVEIYASER
Sbjct: 301 AMITGYAQNGMSEEAIKLFQDMRISSTAPDQITLVGTLSACASIGALDLGKQVEIYASER 360

Query: 361 GLRDDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALF 420
           GLRD+IYV+TALVDMY+KCGSLDNAFRVFD MPRKNEVSWNAMISALAFHGQAQ+A+ALF
Sbjct: 361 GLRDNIYVSTALVDMYSKCGSLDNAFRVFDVMPRKNEVSWNAMISALAFHGQAQKAMALF 420

Query: 421 KSMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLL 480
            SM+ EGGAASPNDITFVGVLSACVHAGLVDEGRR FHMMSSSFGLVPKIEHYSCMVDLL
Sbjct: 421 NSMLNEGGAASPNDITFVGVLSACVHAGLVDEGRRFFHMMSSSFGLVPKIEHYSCMVDLL 480

Query: 481 SRAGHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSGNYV 540
           SRAGHLEEAWDFV+KMPEKPDEVILGALLGACQKRKNV+ISERVMKLLLELEPSNSGNYV
Sbjct: 481 SRAGHLEEAWDFVKKMPEKPDEVILGALLGACQKRKNVEISERVMKLLLELEPSNSGNYV 540

Query: 541 ISSRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQ 600
           ISSRLYANLRRWDDSARMRM+MKQKGVSKTPGCSWIDI+SQLHEFHAGDVLHQEWIEI Q
Sbjct: 541 ISSRLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIQQ 600

Query: 601 ILELLIDDLRREGYIPNTSLL 622
           IL+LLIDDLRREGYIPNT+LL
Sbjct: 601 ILDLLIDDLRREGYIPNTTLL 619

BLAST of Tan0009250 vs. ExPASy TrEMBL
Match: A0A0A0LMG0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G060420 PE=4 SV=1)

HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 562/621 (90.50%), Postives = 585/621 (94.20%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTN 60
           MLR KASPHLISRYLN  K HS   PNPNRQALS K    LQQCL+IKQLKQIH Q+LTN
Sbjct: 1   MLRTKASPHLISRYLNLQKPHS--KPNPNRQALSDKFNSLLQQCLSIKQLKQIHAQLLTN 60

Query: 61  SIHKPNSFLYKIVDLKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSRM 120
           SIHKPNSFLYKI DLKDFAYAS FFS IL+PTEYSFNVMIRGLST WNKSSLALEFYSRM
Sbjct: 61  SIHKPNSFLYKIADLKDFAYASVFFSNILDPTEYSFNVMIRGLSTAWNKSSLALEFYSRM 120

Query: 121 KFLGLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGDL 180
           KFLGLKPN LTYPFLFIACSNLLAV +GR+GH SVI+RGLDEDGHVSHSLITMYARCG +
Sbjct: 121 KFLGLKPNNLTYPFLFIACSNLLAVENGRMGHCSVIRRGLDEDGHVSHSLITMYARCGKM 180

Query: 181 GDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGAC 240
           GDARKVFDEI ++DLVSWNSMISGYSKMR+AGEAVGLFREMMEAGF+PNEM+LVSVLGAC
Sbjct: 181 GDARKVFDEISQKDLVSWNSMISGYSKMRHAGEAVGLFREMMEAGFQPNEMSLVSVLGAC 240

Query: 241 GELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTWN 300
           GELGDLKLGTWVEEFVVENKM LN FMGSALIHMYGKCGDLVSARRIFDSM+KKDKVTWN
Sbjct: 241 GELGDLKLGTWVEEFVVENKMTLNYFMGSALIHMYGKCGDLVSARRIFDSMKKKDKVTWN 300

Query: 301 AMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASER 360
           AMITGYAQNGMSEEAIKLFQDMRMSST PDQITLIG+LSACASIGALDLGKQVEIYASER
Sbjct: 301 AMITGYAQNGMSEEAIKLFQDMRMSSTAPDQITLIGILSACASIGALDLGKQVEIYASER 360

Query: 361 GLRDDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALF 420
           G +DD+YV TALVDMYAKCGSLDNAFRVF GMP KNEVSWNAMISALAFHGQAQEALALF
Sbjct: 361 GFQDDVYVGTALVDMYAKCGSLDNAFRVFYGMPNKNEVSWNAMISALAFHGQAQEALALF 420

Query: 421 KSMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLL 480
           KSM+ EGG  SPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDL 
Sbjct: 421 KSMMNEGGTVSPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLF 480

Query: 481 SRAGHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSGNYV 540
           SRAGHLEEAWDFV  MPEKPDEVILGALLGACQKRKN+DISERVMKLLLELEPSNSGNYV
Sbjct: 481 SRAGHLEEAWDFVMTMPEKPDEVILGALLGACQKRKNIDISERVMKLLLELEPSNSGNYV 540

Query: 541 ISSRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQ 600
           ISS+LYANLRRWDDSARMRM+MKQKGVSKTPGCSWIDI+SQLHEFHAGDVLHQEWIEIHQ
Sbjct: 541 ISSKLYANLRRWDDSARMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQ 600

Query: 601 ILELLIDDLRREGYIPNTSLL 622
           IL+LLIDDLRREGYIPN +LL
Sbjct: 601 ILDLLIDDLRREGYIPNANLL 619

BLAST of Tan0009250 vs. ExPASy TrEMBL
Match: A0A1S3BRJ2 (pentatricopeptide repeat-containing protein At2g34400 OS=Cucumis melo OX=3656 GN=LOC103492726 PE=4 SV=1)

HSP 1 Score: 1127.5 bits (2915), Expect = 0.0e+00
Identity = 557/621 (89.69%), Postives = 583/621 (93.88%), Query Frame = 0

Query: 1   MLRIKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTN 60
           MLRIKASPHLISRY N  K HS PNPNP  QALS+KL   LQQCL+I+QLKQIHTQ+LTN
Sbjct: 1   MLRIKASPHLISRYPNLQKPHSKPNPNP--QALSEKLNSLLQQCLSIRQLKQIHTQLLTN 60

Query: 61  SIHKPNSFLYKIVDLKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSRM 120
           SI+KPNSFLYKIVDLKDFAYAS FFS ILEPTEYSFNVMIRGLST WNK SLALEFYSRM
Sbjct: 61  SIYKPNSFLYKIVDLKDFAYASVFFSNILEPTEYSFNVMIRGLSTAWNKPSLALEFYSRM 120

Query: 121 KFLGLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGDL 180
           KFLGLKPNK TYPFLFIACSNLLAV +GR+GH S IKRGLDEDGHVSHSLITMYARCG +
Sbjct: 121 KFLGLKPNKFTYPFLFIACSNLLAVDNGRMGHCSGIKRGLDEDGHVSHSLITMYARCGKM 180

Query: 181 GDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGAC 240
           GDARKVFDEI ++DLVSWNSMISGYSKMR+AGEAVGLFREMMEAGF+PNEM+LVSVLGAC
Sbjct: 181 GDARKVFDEISQKDLVSWNSMISGYSKMRHAGEAVGLFREMMEAGFQPNEMSLVSVLGAC 240

Query: 241 GELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTWN 300
           GELGDLKLGTWVEEFVVENKM LNSFMGSALIHMYGKCGDLVSARRIFDS +KKDKVTWN
Sbjct: 241 GELGDLKLGTWVEEFVVENKMPLNSFMGSALIHMYGKCGDLVSARRIFDSTKKKDKVTWN 300

Query: 301 AMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASER 360
           AMITGYAQNGMSEEAIKLFQDMR+SST PDQITLIG+LSACASIGALDLGKQ+EIYASER
Sbjct: 301 AMITGYAQNGMSEEAIKLFQDMRVSSTAPDQITLIGILSACASIGALDLGKQLEIYASER 360

Query: 361 GLRDDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALF 420
           G RDDIYV TALVDMYAKCGSLDNAFRVF GMP+KNEVSWNAMISALAFHGQAQEALALF
Sbjct: 361 GYRDDIYVGTALVDMYAKCGSLDNAFRVFYGMPKKNEVSWNAMISALAFHGQAQEALALF 420

Query: 421 KSMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLL 480
           KSM+ EGG  SPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDL 
Sbjct: 421 KSMMNEGGTVSPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLF 480

Query: 481 SRAGHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSGNYV 540
           SRAGHLEEAWDF+  MPEKPDEVILGALLGACQKRKN+DISERVM LLLELEPSNS NYV
Sbjct: 481 SRAGHLEEAWDFIMAMPEKPDEVILGALLGACQKRKNIDISERVMNLLLELEPSNSANYV 540

Query: 541 ISSRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQ 600
           ISS+LYA L RWDDSA MRM+MKQKGVSKTPGCSWIDI+SQLHEFHAGDVLHQEWIEIHQ
Sbjct: 541 ISSKLYAKLGRWDDSAMMRMLMKQKGVSKTPGCSWIDINSQLHEFHAGDVLHQEWIEIHQ 600

Query: 601 ILELLIDDLRREGYIPNTSLL 622
           IL+LLIDDLRREGYIPN +LL
Sbjct: 601 ILDLLIDDLRREGYIPNANLL 619

BLAST of Tan0009250 vs. TAIR 10
Match: AT2G34400.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 677.9 bits (1748), Expect = 7.5e-195
Identity = 340/613 (55.46%), Postives = 439/613 (71.62%), Query Frame = 0

Query: 4   IKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTNSIH 63
           IK      S Y  F K          R  L +  +  L++C+++ QL+QI  QML +S+ 
Sbjct: 7   IKPEKLAFSIYRQFPKFKPRQFEEARRGDLERDFLFLLKKCISVNQLRQIQAQMLLHSVE 66

Query: 64  KPNSFLYKIVDLKDFAYASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSRMKFL 123
           KPN  + K V+L DF Y+S  FS   EP  YSFN MIRGL+ TWN    AL  Y RMKF 
Sbjct: 67  KPNFLIPKAVELGDFNYSSFLFSVTEEPNHYSFNYMIRGLTNTWNDHEAALSLYRRMKFS 126

Query: 124 GLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGDLGDA 183
           GLKP+K TY F+FIAC+ L  +G GR  HSS+ K GL+ D H++HSLI MYA+CG +G A
Sbjct: 127 GLKPDKFTYNFVFIACAKLEEIGVGRSVHSSLFKVGLERDVHINHSLIMMYAKCGQVGYA 186

Query: 184 RKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGEL 243
           RK+FDEI ERD VSWNSMISGYS+  YA +A+ LFR+M E GFEP+E TLVS+LGAC  L
Sbjct: 187 RKLFDEITERDTVSWNSMISGYSEAGYAKDAMDLFRKMEEEGFEPDERTLVSMLGACSHL 246

Query: 244 GDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTWNAMI 303
           GDL+ G  +EE  +  K+ L++F+GS LI MYGKCGDL SARR+F+ M KKD+V W AMI
Sbjct: 247 GDLRTGRLLEEMAITKKIGLSTFLGSKLISMYGKCGDLDSARRVFNQMIKKDRVAWTAMI 306

Query: 304 TGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGLR 363
           T Y+QNG S EA KLF +M  +  +PD  TL  +LSAC S+GAL+LGKQ+E +ASE  L+
Sbjct: 307 TVYSQNGKSSEAFKLFFEMEKTGVSPDAGTLSTVLSACGSVGALELGKQIETHASELSLQ 366

Query: 364 DDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALFKSM 423
            +IYVAT LVDMY KCG ++ A RVF+ MP KNE +WNAMI+A A  G A+EAL LF  M
Sbjct: 367 HNIYVATGLVDMYGKCGRVEEALRVFEAMPVKNEATWNAMITAYAHQGHAKEALLLFDRM 426

Query: 424 IKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLLSRA 483
                +  P+DITF+GVLSACVHAGLV +G R FH MSS FGLVPKIEHY+ ++DLLSRA
Sbjct: 427 -----SVPPSDITFIGVLSACVHAGLVHQGCRYFHEMSSMFGLVPKIEHYTNIIDLLSRA 486

Query: 484 GHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLEL-EPSNSGNYVIS 543
           G L+EAW+F+E+ P KPDE++L A+LGAC KRK+V I E+ M++L+E+ E  N+GNYVIS
Sbjct: 487 GMLDEAWEFMERFPGKPDEIMLAAILGACHKRKDVAIREKAMRMLMEMKEAKNAGNYVIS 546

Query: 544 SRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHAG-DVLHQEWIEIHQI 603
           S + A+++ WD+SA+MR +M+ +GV KTPGCSWI+I+ +L EF AG D L     +   +
Sbjct: 547 SNVLADMKMWDESAKMRALMRDRGVVKTPGCSWIEIEGELMEFLAGSDYLQCGREDSGSL 606

Query: 604 LELLIDDLRREGY 615
            +LL+++++RE Y
Sbjct: 607 FDLLVEEMKRERY 614

BLAST of Tan0009250 vs. TAIR 10
Match: AT1G08070.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 447.2 bits (1149), Expect = 2.2e-125
Identity = 238/622 (38.26%), Postives = 365/622 (58.68%), Query Frame = 0

Query: 38  VGFLQQCLTIKQLKQIHTQMLTNSIHKPNSFLYKIVD-------LKDFAYASDFFSKILE 97
           +  L  C T++ L+ IH QM+   +H  N  L K+++        +   YA   F  I E
Sbjct: 37  LSLLHNCKTLQSLRIIHAQMIKIGLHNTNYALSKLIEFCILSPHFEGLPYAISVFKTIQE 96

Query: 98  PTEYSFNVMIRGLSTTWNKSSLALEFYSRMKFLGLKPNKLTYPFLFIACSNLLAVGHGRV 157
           P    +N M RG + + +  S AL+ Y  M  LGL PN  T+PF+  +C+   A   G+ 
Sbjct: 97  PNLLIWNTMFRGHALSSDPVS-ALKLYVCMISLGLLPNSYTFPFVLKSCAKSKAFKEGQQ 156

Query: 158 GHSSVIKRGLDEDGHVSHSLITMYARCGDLGDARKV------------------------ 217
            H  V+K G D D +V  SLI+MY + G L DA KV                        
Sbjct: 157 IHGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPHRDVVSYTALIKGYASRGY 216

Query: 218 -------FDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGA 277
                  FDEIP +D+VSWN+MISGY++     EA+ LF++MM+    P+E T+V+V+ A
Sbjct: 217 IENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEALELFKDMMKTNVRPDESTMVTVVSA 276

Query: 278 CGELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTW 337
           C + G ++LG  V  ++ ++    N  + +ALI +Y KCG+L +A  +F+ +  KD ++W
Sbjct: 277 CAQSGSIELGRQVHLWIDDHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKDVISW 336

Query: 338 NAMITGYAQNGMSEEAIKLFQDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASE 397
           N +I GY    + +EA+ LFQ+M  S  TP+ +T++ +L ACA +GA+D+G+ + +Y  +
Sbjct: 337 NTLIGGYTHMNLYKEALLLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDK 396

Query: 398 R--GLRDDIYVATALVDMYAKCGSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEAL 457
           R  G+ +   + T+L+DMYAKCG ++ A +VF+ +  K+  SWNAMI   A HG+A  + 
Sbjct: 397 RLKGVTNASSLRTSLIDMYAKCGDIEAAHQVFNSILHKSLSSWNAMIFGFAMHGRADASF 456

Query: 458 ALFKSMIKEGGAASPNDITFVGVLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMV 517
            LF  M K G    P+DITFVG+LSAC H+G++D GR +F  M+  + + PK+EHY CM+
Sbjct: 457 DLFSRMRKIG--IQPDDITFVGLLSACSHSGMLDLGRHIFRTMTQDYKMTPKLEHYGCMI 516

Query: 518 DLLSRAGHLEEAWDFVEKMPEKPDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSG 577
           DLL  +G  +EA + +  M  +PD VI  +LL AC+   NV++ E   + L+++EP N G
Sbjct: 517 DLLGHSGLFKEAEEMINMMEMEPDGVIWCSLLKACKMHGNVELGESFAENLIKIEPENPG 576

Query: 578 NYVISSRLYANLRRWDDSARMRMMMKQKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIE 620
           +YV+ S +YA+  RW++ A+ R ++  KG+ K PGCS I+IDS +HEF  GD  H    E
Sbjct: 577 SYVLLSNIYASAGRWNEVAKTRALLNDKGMKKVPGCSSIEIDSVVHEFIIGDKFHPRNRE 636

BLAST of Tan0009250 vs. TAIR 10
Match: AT2G29760.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 435.6 bits (1119), Expect = 6.5e-122
Identity = 234/652 (35.89%), Postives = 371/652 (56.90%), Query Frame = 0

Query: 6   ASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQML-TNSIHK 65
           A P  + R+ NF    SNPN  P       + +  +++C++++QLKQ H  M+ T +   
Sbjct: 7   AQPLSLPRHPNF----SNPN-QPTTNNERSRHISLIERCVSLRQLKQTHGHMIRTGTFSD 66

Query: 66  PNSF--LYKIVDLKDFA---YASDFFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSR 125
           P S   L+ +  L  FA   YA   F +I +P  +++N +IR  ++  +       F   
Sbjct: 67  PYSASKLFAMAALSSFASLEYARKVFDEIPKPNSFAWNTLIRAYASGPDPVLSIWAFLDM 126

Query: 126 MKFLGLKPNKLTYPFLFIACSNLLAVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGD 185
           +      PNK T+PFL  A + + ++  G+  H   +K  +  D  V++SLI  Y  CGD
Sbjct: 127 VSESQCYPNKYTFPFLIKAAAEVSSLSLGQSLHGMAVKSAVGSDVFVANSLIHCYFSCGD 186

Query: 186 LGDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGA 245
           L  A KVF  I E+D+VSWNSMI+G+ +     +A+ LF++M     + + +T+V VL A
Sbjct: 187 LDSACKVFTTIKEKDVVSWNSMINGFVQKGSPDKALELFKKMESEDVKASHVTMVGVLSA 246

Query: 246 CGELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVT- 305
           C ++ +L+ G  V  ++ EN++ +N  + +A++ MY KCG +  A+R+FD+M +KD VT 
Sbjct: 247 CAKIRNLEFGRQVCSYIEENRVNVNLTLANAMLDMYTKCGSIEDAKRLFDAMEEKDNVTW 306

Query: 306 ------------------------------WNAMITGYAQNGMSEEAIKLFQDMRMSSTT 365
                                         WNA+I+ Y QNG   EA+ +F ++++    
Sbjct: 307 TTMLDGYAISEDYEAAREVLNSMPQKDIVAWNALISAYEQNGKPNEALIVFHELQLQKNM 366

Query: 366 P-DQITLIGMLSACASIGALDLGKQVEIYASERGLRDDIYVATALVDMYAKCGSLDNAFR 425
             +QITL+  LSACA +GAL+LG+ +  Y  + G+R + +V +AL+ MY+KCG L+ +  
Sbjct: 367 KLNQITLVSTLSACAQVGALELGRWIHSYIKKHGIRMNFHVTSALIHMYSKCGDLEKSRE 426

Query: 426 VFDGMPRKNEVSWNAMISALAFHGQAQEALALFKSMIKEGGAASPNDITFVGVLSACVHA 485
           VF+ + +++   W+AMI  LA HG   EA+ +F  M  +     PN +TF  V  AC H 
Sbjct: 427 VFNSVEKRDVFVWSAMIGGLAMHGCGNEAVDMFYKM--QEANVKPNGVTFTNVFCACSHT 486

Query: 486 GLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLLSRAGHLEEAWDFVEKMPEKPDEVILGA 545
           GLVDE   LFH M S++G+VP+ +HY+C+VD+L R+G+LE+A  F+E MP  P   + GA
Sbjct: 487 GLVDEAESLFHQMESNYGIVPEEKHYACIVDVLGRSGYLEKAVKFIEAMPIPPSTSVWGA 546

Query: 546 LLGACQKRKNVDISERVMKLLLELEPSNSGNYVISSRLYANLRRWDDSARMRMMMKQKGV 605
           LLGAC+   N++++E     LLELEP N G +V+ S +YA L +W++ + +R  M+  G+
Sbjct: 547 LLGACKIHANLNLAEMACTRLLELEPRNDGAHVLLSNIYAKLGKWENVSELRKHMRVTGL 606

Query: 606 SKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQILELLIDDLRREGYIPNTS 620
            K PGCS I+ID  +HEF +GD  H    +++  L  +++ L+  GY P  S
Sbjct: 607 KKEPGCSSIEIDGMIHEFLSGDNAHPMSEKVYGKLHEVMEKLKSNGYEPEIS 651

BLAST of Tan0009250 vs. TAIR 10
Match: AT2G22410.1 (SLOW GROWTH 1 )

HSP 1 Score: 433.3 bits (1113), Expect = 3.2e-121
Identity = 249/646 (38.54%), Postives = 373/646 (57.74%), Query Frame = 0

Query: 3   RIKASPHLISRYLNFDKTHSNPNPNPNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTNSI 62
           R ++ PH   + +N++ THS    NP        L+  L++C  +  LKQI  QM+ N +
Sbjct: 30  RTRSLPHHRDKPINWNSTHSFVLHNP--------LLSLLEKCKLLLHLKQIQAQMIINGL 89

Query: 63  ---HKPNSFLYKIVDLKDFAYASDFFSKILE----PTEYSFNVMIRGLSTTWNKSSLALE 122
                 +S L     L +  Y  D+  KIL+    P  +S+NV IRG S + N     L 
Sbjct: 90  ILDPFASSRLIAFCALSESRYL-DYSVKILKGIENPNIFSWNVTIRGFSESENPKESFL- 149

Query: 123 FYSRMKFLGL---KPNKLTYPFLFIACSN--LLAVGHGRVGHSSVIKRGLDEDGHVSHSL 182
            Y +M   G    +P+  TYP LF  C++  L ++GH  +GH  V+K  L+   HV ++ 
Sbjct: 150 LYKQMLRHGCCESRPDHFTYPVLFKVCADLRLSSLGHMILGH--VLKLRLELVSHVHNAS 209

Query: 183 ITMYARCGDLGDARKVFDEIPERDLVSWNSMISGYSKMRYAGEAVGLFREMMEAGFEPNE 242
           I M+A CGD+ +ARKVFDE P RDLVSWN +I+GY K+  A +A+ +++ M   G +P++
Sbjct: 210 IHMFASCGDMENARKVFDESPVRDLVSWNCLINGYKKIGEAEKAIYVYKLMESEGVKPDD 269

Query: 243 MTLVSVLGACGELGDLKLGTWVEEFVVENKMVLNSFMGSALIHMYGKCGDLVSARRIFDS 302
           +T++ ++ +C  LGDL  G    E+V EN + +   + +AL+ M+ KCGD+  ARRIFD+
Sbjct: 270 VTMIGLVSSCSMLGDLNRGKEFYEYVKENGLRMTIPLVNALMDMFSKCGDIHEARRIFDN 329

Query: 303 MRKKDKVTWNAMITGYAQNGM-------------------------------SEEAIKLF 362
           + K+  V+W  MI+GYA+ G+                                ++A+ LF
Sbjct: 330 LEKRTIVSWTTMISGYARCGLLDVSRKLFDDMEEKDVVLWNAMIGGSVQAKRGQDALALF 389

Query: 363 QDMRMSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGLRDDIYVATALVDMYAKC 422
           Q+M+ S+T PD+IT+I  LSAC+ +GALD+G  +  Y  +  L  ++ + T+LVDMYAKC
Sbjct: 390 QEMQTSNTKPDEITMIHCLSACSQLGALDVGIWIHRYIEKYSLSLNVALGTSLVDMYAKC 449

Query: 423 GSLDNAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALFKSMIKEGGAASPNDITFVG 482
           G++  A  VF G+  +N +++ A+I  LA HG A  A++ F  MI  G   +P++ITF+G
Sbjct: 450 GNISEALSVFHGIQTRNSLTYTAIIGGLALHGDASTAISYFNEMIDAG--IAPDEITFIG 509

Query: 483 VLSACVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLLSRAGHLEEAWDFVEKMPEK 542
           +LSAC H G++  GR  F  M S F L P+++HYS MVDLL RAG LEEA   +E MP +
Sbjct: 510 LLSACCHGGMIQTGRDYFSQMKSRFNLNPQLKHYSIMVDLLGRAGLLEEADRLMESMPME 569

Query: 543 PDEVILGALLGACQKRKNVDISERVMKLLLELEPSNSGNYVISSRLYANLRRWDDSARMR 602
            D  + GALL  C+   NV++ E+  K LLEL+PS+SG YV+   +Y     W+D+ R R
Sbjct: 570 ADAAVWGALLFGCRMHGNVELGEKAAKKLLELDPSDSGIYVLLDGMYGEANMWEDAKRAR 629

Query: 603 MMMKQKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQILELL 606
            MM ++GV K PGCS I+++  + EF   D    E  +I+  L  L
Sbjct: 630 RMMNERGVEKIPGCSSIEVNGIVCEFIVRDKSRPESEKIYDRLHCL 661

BLAST of Tan0009250 vs. TAIR 10
Match: AT3G08820.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 428.3 bits (1100), Expect = 1.0e-119
Identity = 225/598 (37.63%), Postives = 352/598 (58.86%), Query Frame = 0

Query: 28  PNRQALSQKLVGFLQQCLTIKQLKQIHTQMLTNSIHKP----NSFLYKIVDLKDFAYASD 87
           P+  +  Q++   +    T+  LKQIH  ++ + +H      N  L + +  +   Y+  
Sbjct: 7   PSATSKVQQIKTLISVACTVNHLKQIHVSLINHHLHHDTFLVNLLLKRTLFFRQTKYSYL 66

Query: 88  FFSKILEPTEYSFNVMIRGLSTTWNKSSLALEFYSRMKFLGLKPNKLTYPFLFIACSNLL 147
            FS    P  + +N +I G     +     L+ +  ++  GL  +  T+P +  AC+   
Sbjct: 67  LFSHTQFPNIFLYNSLINGFVNN-HLFHETLDLFLSIRKHGLYLHGFTFPLVLKACTRAS 126

Query: 148 AVGHGRVGHSSVIKRGLDEDGHVSHSLITMYARCGDLGDARKVFDEIPERDLVSWNSMIS 207
           +   G   HS V+K G + D     SL+++Y+  G L DA K+FDEIP+R +V+W ++ S
Sbjct: 127 SRKLGIDLHSLVVKCGFNHDVAAMTSLLSIYSGSGRLNDAHKLFDEIPDRSVVTWTALFS 186

Query: 208 GYSKMRYAGEAVGLFREMMEAGFEPNEMTLVSVLGACGELGDLKLGTWVEEFVVENKMVL 267
           GY+      EA+ LF++M+E G +P+   +V VL AC  +GDL  G W+ +++ E +M  
Sbjct: 187 GYTTSGRHREAIDLFKKMVEMGVKPDSYFIVQVLSACVHVGDLDSGEWIVKYMEEMEMQK 246

Query: 268 NSFMGSALIHMYGKCGDLVSARRIFDSMRKKDKVTWNAMITGYAQNGMSEEAIKLFQDMR 327
           NSF+ + L+++Y KCG +  AR +FDSM +KD VTW+ MI GYA N   +E I+LF  M 
Sbjct: 247 NSFVRTTLVNLYAKCGKMEKARSVFDSMVEKDIVTWSTMIQGYASNSFPKEGIELFLQML 306

Query: 328 MSSTTPDQITLIGMLSACASIGALDLGKQVEIYASERGLRDDIYVATALVDMYAKCGSLD 387
             +  PDQ +++G LS+CAS+GALDLG+             ++++A AL+DMYAKCG++ 
Sbjct: 307 QENLKPDQFSIVGFLSSCASLGALDLGEWGISLIDRHEFLTNLFMANALIDMYAKCGAMA 366

Query: 388 NAFRVFDGMPRKNEVSWNAMISALAFHGQAQEALALFKSMIKEGGAASPNDITFVGVLSA 447
             F VF  M  K+ V  NA IS LA +G  + + A+F    K G   SP+  TF+G+L  
Sbjct: 367 RGFEVFKEMKEKDIVIMNAAISGLAKNGHVKLSFAVFGQTEKLG--ISPDGSTFLGLLCG 426

Query: 448 CVHAGLVDEGRRLFHMMSSSFGLVPKIEHYSCMVDLLSRAGHLEEAWDFVEKMPEKPDEV 507
           CVHAGL+ +G R F+ +S  + L   +EHY CMVDL  RAG L++A+  +  MP +P+ +
Sbjct: 427 CVHAGLIQDGLRFFNAISCVYALKRTVEHYGCMVDLWGRAGMLDDAYRLICDMPMRPNAI 486

Query: 508 ILGALLGACQKRKNVDISERVMKLLLELEPSNSGNYVISSRLYANLRRWDDSARMRMMMK 567
           + GALL  C+  K+  ++E V+K L+ LEP N+GNYV  S +Y+   RWD++A +R MM 
Sbjct: 487 VWGALLSGCRLVKDTQLAETVLKELIALEPWNAGNYVQLSNIYSVGGRWDEAAEVRDMMN 546

Query: 568 QKGVSKTPGCSWIDIDSQLHEFHAGDVLHQEWIEIHQILELLIDDLRREGYIPNTSLL 622
           +KG+ K PG SWI+++ ++HEF A D  H    +I+  LE L +++R  G++P T  +
Sbjct: 547 KKGMKKIPGYSWIELEGKVHEFLADDKSHPLSDKIYAKLEDLGNEMRLMGFVPTTEFV 601

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O647051.1e-19355.46Pentatricopeptide repeat-containing protein At2g34400 OS=Arabidopsis thaliana OX... [more]
Q9LN013.0e-12438.26Pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Arabidop... [more]
O823809.1e-12135.89Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidop... [more]
Q9SJZ34.5e-12038.54Pentatricopeptide repeat-containing protein At2g22410, mitochondrial OS=Arabidop... [more]
Q9SR821.5e-11837.63Putative pentatricopeptide repeat-containing protein At3g08820 OS=Arabidopsis th... [more]
Match NameE-valueIdentityDescription
XP_022929625.10.0e+0092.91pentatricopeptide repeat-containing protein At2g34400 [Cucurbita moschata][more]
XP_023521362.10.0e+0092.75pentatricopeptide repeat-containing protein At2g34400-like [Cucurbita pepo subsp... [more]
XP_022153282.10.0e+0093.26pentatricopeptide repeat-containing protein At2g34400 [Momordica charantia][more]
XP_022997564.10.0e+0092.43pentatricopeptide repeat-containing protein At2g34400 [Cucurbita maxima][more]
XP_038889591.10.0e+0093.24pentatricopeptide repeat-containing protein At2g34400 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1EUW30.0e+0092.91pentatricopeptide repeat-containing protein At2g34400 OS=Cucurbita moschata OX=3... [more]
A0A6J1DIG60.0e+0093.26pentatricopeptide repeat-containing protein At2g34400 OS=Momordica charantia OX=... [more]
A0A6J1KBU40.0e+0092.43pentatricopeptide repeat-containing protein At2g34400 OS=Cucurbita maxima OX=366... [more]
A0A0A0LMG00.0e+0090.50Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G060420 PE=4 SV=1[more]
A0A1S3BRJ20.0e+0089.69pentatricopeptide repeat-containing protein At2g34400 OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT2G34400.17.5e-19555.46Pentatricopeptide repeat (PPR-like) superfamily protein [more]
AT1G08070.12.2e-12538.26Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G29760.16.5e-12235.89Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G22410.13.2e-12138.54SLOW GROWTH 1 [more]
AT3G08820.11.0e-11937.63Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 29..150
e-value: 1.6E-6
score: 29.6
coord: 152..246
e-value: 5.9E-23
score: 83.1
coord: 247..355
e-value: 2.0E-25
score: 91.2
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 356..429
e-value: 5.4E-16
score: 60.8
coord: 430..594
e-value: 2.7E-27
score: 98.0
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 472..499
e-value: 0.0011
score: 19.0
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 194..240
e-value: 7.6E-10
score: 38.8
coord: 395..445
e-value: 2.0E-9
score: 37.5
coord: 91..139
e-value: 2.0E-7
score: 31.1
coord: 294..342
e-value: 5.3E-13
score: 49.0
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 270..294
e-value: 8.0E-4
score: 17.4
coord: 473..498
e-value: 4.0E-4
score: 18.4
coord: 398..427
e-value: 8.4E-5
score: 20.5
coord: 435..468
e-value: 1.2E-4
score: 20.0
coord: 196..230
e-value: 1.0E-9
score: 36.0
coord: 297..330
e-value: 3.5E-9
score: 34.3
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 365..395
score: 8.845827
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 295..329
score: 12.956314
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 92..127
score: 9.525427
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 264..294
score: 8.571795
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 194..228
score: 13.153618
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 396..430
score: 10.906551
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 469..499
score: 8.834866
NoneNo IPR availablePANTHERPTHR47926:SF54SUBFAMILY NOT NAMEDcoord: 192..291
coord: 291..619
coord: 50..191
NoneNo IPR availablePANTHERPTHR47926PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 192..291
coord: 291..619
coord: 50..191

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0009250.1Tan0009250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding