Tan0009200 (gene) Snake gourd v1

Overview
NameTan0009200
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionisoflavone reductase-like protein
LocationLG05: 15105395 .. 15107384 (-)
RNA-Seq ExpressionTan0009200
SyntenyTan0009200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTGTCTATGATCAGATTTTTGTTCGCCGGGAATCTTAATCCGATGGCTCAGAAACTCAAGGTTCTCATCATCGGAGGAACTGGCTACATCGGAAAATTTATAGTGGAAGCCAGCGCCAAAGCAGGTCATTCAACTTATGCATTGGTCCGGGAATCCACGCTCGCTAATTCCGCCAAGTCTCCGATCATCGATAATTTCAGGAGCTTGGGGGTCAATTTCCTCATCGTAAGTTCATTCATTATCCTCTATTTCTCTCGATTTTGTTGTTTGATTGTTGAGAATGTTCAGGAAGATTATGGAATCGATTACTGAAATGTGAAATGTTCCCTAATTTTCTTGATTGCCTATTTTGTTCCCCCAATAACTGAAGTAGGTTTCTAGTTCAATTCCGCCGAATTTGAAGTGATCATTTCATTTTCTGTTTGGATTCTCTAAAACGAGTAACGAATAATGTCATTCACGTTATAATCTTGGGAAGTGATAATGTTCGATTTGCTAAAACGCTGAGAAATTGACGCAGGGCGATATCTTCGACAATGAGAGTCTGGTTAAGGCAATAAAGCATGTTGATGTTGTGATATCCACCGTTGGTCGTGCTCTGTTAGCCCATCAAGAGAAGATCATATCTGCTATCAAACAAGCTGGTAATGTGAAGGTAACCTTCATTTCACCACTGTCATTTCCTCGCCAAACTTGTTCAATGTTTTTCCATTGATTGTTGTTACTGCAAGATGCCTTCTGCTAGGAGAGATTGTGTGGGGTTGGAAAATTTTGAGCATTAAAATGTTTTTGGTACAGAGATTCTTCCCCTCGGAGTTTGGCAACGATGTGGATCGTGTTCACGCAGTGGAGCCGGCAAAATCAATGTTTGATGCTAAGGTTGAAATTCGTAGGGCTGTTGAGGCAGAGGGAATCCCCCATACATTTGTGGTATGCAATTTCTTTGATGGTTACTTTCTTCGTAACTTTTGTCAGCCAGGAGCCACTGATCCACCTAGGGACAAAATCAAAATCATTGGGGATGGAAATCCCAAAGGTATAATTATGCTGATTCTAATCTCATGAGTTGCTGGATTATTGTTGTGTTGTATAGCAGTGTAATCTCATTTTCTTGAGCTGCAATGGTGTTCCAAAATGAAAACATTCCAAGAAATTCAAGAGATATTAGTATATCATTGGTTTTTTTTTCTTTTTTTAATCAAAAACTTAATGCATAATGGACTGCAACATGAAGCATCTTCATTCATGACCAGTTTCTTGAAGGGAGAAATGGATTTGGTTTCAGCCGCACCTCTCATACTATTCATTTATTATGTATCTGTTTTGTTTGCTTAAGTTATCTACAACAAGGAAGAAGACATTGGCACATACACCATCCGTGCCATAGAGGATCCAAGGACCCTCAACAAGATCTTGTACATTAGACCTCCTGCCAATATCTACTCGACTAACGAGCTAGTATCGCTATGGGAGAGAAAAATAGGCTCGACTCTTGAAAGGATCTACATCCCAGAAGAGGAGCTGTTGAAGAACATACAAGGTTGGTTTGGGCAAAACAACAAGCTCTCAATTCTACCATGCTTCTAAAATAAAAAGACTAAAACTCATTGGTATCTGCTATAATCTTCTTTTGCAGAGGCTCCATTTCCACTTAACACAGTATTAGCAATTAGTCACACTGCATTTGTGAAGGGAGATCATACCAACTTTGATATTGAACCATCATTTGGAGTGGAAGCTTCAGAGCTGTACTCAGATGTTCAATACGCAACTGTGGAAGAATACCTCAACCAGTTTGTTTAGAGAGCAAACAACCCACCCCTGATGTTCAAGCCTAGGTCTTTTACTTTATTGATTTAATGGGTGTAAGCAGTTCTGTCCTTTATATATGTGTAATGGAAAAAATGATCTCATTTGAAAACAGAGGTTCTTGTAGATGGATTCACTGACCCAAAATTGCATTTTTTGAAAGATGATAGTTAACAAA

mRNA sequence

CTTCTGTCTATGATCAGATTTTTGTTCGCCGGGAATCTTAATCCGATGGCTCAGAAACTCAAGGTTCTCATCATCGGAGGAACTGGCTACATCGGAAAATTTATAGTGGAAGCCAGCGCCAAAGCAGGTCATTCAACTTATGCATTGGTCCGGGAATCCACGCTCGCTAATTCCGCCAAGTCTCCGATCATCGATAATTTCAGGAGCTTGGGGGTCAATTTCCTCATCGGCGATATCTTCGACAATGAGAGTCTGGTTAAGGCAATAAAGCATGTTGATGTTGTGATATCCACCGTTGGTCGTGCTCTGTTAGCCCATCAAGAGAAGATCATATCTGCTATCAAACAAGCTGGTAATGTGAAGAGATTCTTCCCCTCGGAGTTTGGCAACGATGTGGATCGTGTTCACGCAGTGGAGCCGGCAAAATCAATGTTTGATGCTAAGGTTGAAATTCGTAGGGCTGTTGAGGCAGAGGGAATCCCCCATACATTTGTGGTATGCAATTTCTTTGATGGTTACTTTCTTCGTAACTTTTGTCAGCCAGGAGCCACTGATCCACCTAGGGACAAAATCAAAATCATTGGGGATGGAAATCCCAAAGTTATCTACAACAAGGAAGAAGACATTGGCACATACACCATCCGTGCCATAGAGGATCCAAGGACCCTCAACAAGATCTTGTACATTAGACCTCCTGCCAATATCTACTCGACTAACGAGCTAGTATCGCTATGGGAGAGAAAAATAGGCTCGACTCTTGAAAGGATCTACATCCCAGAAGAGGAGCTGTTGAAGAACATACAAGAGGCTCCATTTCCACTTAACACAGTATTAGCAATTAGTCACACTGCATTTGTGAAGGGAGATCATACCAACTTTGATATTGAACCATCATTTGGAGTGGAAGCTTCAGAGCTGTACTCAGATGTTCAATACGCAACTGTGGAAGAATACCTCAACCAGTTTGTTTAGAGAGCAAACAACCCACCCCTGATGTTCAAGCCTAGGTCTTTTACTTTATTGATTTAATGGGTGTAAGCAGTTCTGTCCTTTATATATGTGTAATGGAAAAAATGATCTCATTTGAAAACAGAGGTTCTTGTAGATGGATTCACTGACCCAAAATTGCATTTTTTGAAAGATGATAGTTAACAAA

Coding sequence (CDS)

ATGATCAGATTTTTGTTCGCCGGGAATCTTAATCCGATGGCTCAGAAACTCAAGGTTCTCATCATCGGAGGAACTGGCTACATCGGAAAATTTATAGTGGAAGCCAGCGCCAAAGCAGGTCATTCAACTTATGCATTGGTCCGGGAATCCACGCTCGCTAATTCCGCCAAGTCTCCGATCATCGATAATTTCAGGAGCTTGGGGGTCAATTTCCTCATCGGCGATATCTTCGACAATGAGAGTCTGGTTAAGGCAATAAAGCATGTTGATGTTGTGATATCCACCGTTGGTCGTGCTCTGTTAGCCCATCAAGAGAAGATCATATCTGCTATCAAACAAGCTGGTAATGTGAAGAGATTCTTCCCCTCGGAGTTTGGCAACGATGTGGATCGTGTTCACGCAGTGGAGCCGGCAAAATCAATGTTTGATGCTAAGGTTGAAATTCGTAGGGCTGTTGAGGCAGAGGGAATCCCCCATACATTTGTGGTATGCAATTTCTTTGATGGTTACTTTCTTCGTAACTTTTGTCAGCCAGGAGCCACTGATCCACCTAGGGACAAAATCAAAATCATTGGGGATGGAAATCCCAAAGTTATCTACAACAAGGAAGAAGACATTGGCACATACACCATCCGTGCCATAGAGGATCCAAGGACCCTCAACAAGATCTTGTACATTAGACCTCCTGCCAATATCTACTCGACTAACGAGCTAGTATCGCTATGGGAGAGAAAAATAGGCTCGACTCTTGAAAGGATCTACATCCCAGAAGAGGAGCTGTTGAAGAACATACAAGAGGCTCCATTTCCACTTAACACAGTATTAGCAATTAGTCACACTGCATTTGTGAAGGGAGATCATACCAACTTTGATATTGAACCATCATTTGGAGTGGAAGCTTCAGAGCTGTACTCAGATGTTCAATACGCAACTGTGGAAGAATACCTCAACCAGTTTGTTTAG

Protein sequence

MIRFLFAGNLNPMAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFLIGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRVHAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIGDGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLERIYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATVEEYLNQFV
Homology
BLAST of Tan0009200 vs. ExPASy Swiss-Prot
Match: B9HRL7 (Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=PCBER PE=1 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 2.4e-133
Identity = 229/303 (75.58%), Postives = 266/303 (87.79%), Query Frame = 0

Query: 18  KVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFLIGDIF 77
           K+L IGGTGYIGKFIVEASAKAGH T+ LVRESTL+N AKS +I NF++LGVNFLIGD+F
Sbjct: 4   KILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSNPAKSVVIYNFKNLGVNFLIGDLF 63

Query: 78  DNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRVHAVEP 137
           D+ESLVKAIK VDVVISTVG A L  Q++II+AIK+AGNVKRFFPSEFGNDVDRV+AVEP
Sbjct: 64  DHESLVKAIKQVDVVISTVGHAQLVEQDRIIAAIKEAGNVKRFFPSEFGNDVDRVNAVEP 123

Query: 138 AKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIGDGNPK 197
           AKS F  K  +RRA+EAEGIP+T+V  NFF GYFL +F QPGAT PPRDK+ I+GDGNPK
Sbjct: 124 AKSAFATKANVRRAIEAEGIPYTYVSSNFFSGYFLLSFNQPGATAPPRDKVVILGDGNPK 183

Query: 198 VIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLERIYIPE 257
            ++NKE+DI TYTI+A++DPRTLNKILYI+PPAN  S N+LVSLWE+KIG TLERIY+PE
Sbjct: 184 AVFNKEDDIATYTIKAVDDPRTLNKILYIKPPANTISFNDLVSLWEKKIGKTLERIYVPE 243

Query: 258 EELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATVEEYLN 317
           E+LLKNIQEA  P+N VL+I H+ FVKGDHTNF+IEPSFGVEASELY DV+Y TV+EYL 
Sbjct: 244 EQLLKNIQEASVPVNVVLSIGHSVFVKGDHTNFEIEPSFGVEASELYPDVKYTTVDEYLK 303

Query: 318 QFV 321
           QFV
Sbjct: 304 QFV 306

BLAST of Tan0009200 vs. ExPASy Swiss-Prot
Match: O81355 (Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC5 PE=1 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 7.0e-133
Identity = 229/308 (74.35%), Postives = 268/308 (87.01%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           MA K ++L IGGTGYIGKFIVEASAKAG+ TY LVRE++L++ AKS +I+NF++LGVNF+
Sbjct: 1   MASKSQILFIGGTGYIGKFIVEASAKAGYPTYVLVREASLSDPAKSKVIENFKALGVNFV 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
           +GD++D+ESLVKAIK VDVVISTVG   LA Q KII+AIK+AGNVKRFFPSEFGNDVDR 
Sbjct: 61  LGDLYDHESLVKAIKQVDVVISTVGHGQLADQGKIIAAIKEAGNVKRFFPSEFGNDVDRS 120

Query: 133 HAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 192
           HAVEPAKS F+ K +IRRAVEAEGIP+T+V  NFF GYFL    QPGA+  PRDK+ I+G
Sbjct: 121 HAVEPAKSAFETKAKIRRAVEAEGIPYTYVSSNFFAGYFLPTLNQPGASSAPRDKVVILG 180

Query: 193 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 252
           DGNPK I+NKE+DIGTYTIRA++DPRTLNK+LYIRPPAN  S NELVSLWE+KIG TLER
Sbjct: 181 DGNPKAIFNKEDDIGTYTIRAVDDPRTLNKVLYIRPPANTISFNELVSLWEKKIGKTLER 240

Query: 253 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 312
           IY+PEE+LLKNIQEA  PLN +L+ISH  FVKGDHTNF+IEPSFGVEA+ LY DV+Y TV
Sbjct: 241 IYVPEEQLLKNIQEAAVPLNVILSISHAVFVKGDHTNFEIEPSFGVEATALYPDVKYTTV 300

Query: 313 EEYLNQFV 321
           +EYLNQFV
Sbjct: 301 DEYLNQFV 308

BLAST of Tan0009200 vs. ExPASy Swiss-Prot
Match: Q9FUW6 (Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6 PE=1 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 7.5e-127
Identity = 216/308 (70.13%), Postives = 259/308 (84.09%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           MA K K+LIIGGTGYIGKFIVEASAK+GH T+ALVREST+++  K  +++ F+ LGV  L
Sbjct: 1   MAHKSKILIIGGTGYIGKFIVEASAKSGHPTFALVRESTVSDPVKGKLVEKFKGLGVTLL 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
            GD++D+ESLVKA K VDVVISTVG   LA Q KII+AIK+AGN+KRFFPSEFGNDVDRV
Sbjct: 61  HGDLYDHESLVKAFKQVDVVISTVGHLQLADQVKIIAAIKEAGNIKRFFPSEFGNDVDRV 120

Query: 133 HAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 192
           HAVEPAK+ F  K EIRR  EAEGIP+T+V  NFF GYFL    QPG T PPR+K+ I G
Sbjct: 121 HAVEPAKTAFATKAEIRRKTEAEGIPYTYVSSNFFAGYFLPTLAQPGLTSPPREKVVIFG 180

Query: 193 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 252
           DGN + ++NKE+DIGTYTIRA++DPRTLNKI+YI+P  NIYS NE+V+LWE+KIG TLE+
Sbjct: 181 DGNARAVFNKEDDIGTYTIRAVDDPRTLNKIVYIKPAKNIYSFNEIVALWEKKIGKTLEK 240

Query: 253 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 312
           IY+PEE+LLK+IQE+P P+N +LAI+H+ FVKGDHTNF+IE SFGVEASELY DV+Y TV
Sbjct: 241 IYVPEEKLLKDIQESPIPINVILAINHSVFVKGDHTNFEIEASFGVEASELYPDVKYTTV 300

Query: 313 EEYLNQFV 321
           EEYL QFV
Sbjct: 301 EEYLQQFV 308

BLAST of Tan0009200 vs. ExPASy Swiss-Prot
Match: B2WSN0 (Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 1.8e-125
Identity = 218/309 (70.55%), Postives = 262/309 (84.79%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           M  K K+LIIGGTGYIGKFIVEAS K GH T+ALVRE+T+++  K  +++ F++LGV+ L
Sbjct: 1   MGSKSKILIIGGTGYIGKFIVEASVKEGHPTFALVRETTVSDPVKGKLVEKFQNLGVSLL 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
            GD++D++SLVKAIK VDVVISTVG   +A Q KII+AIK+AGNVKRFFPSEFGNDVD V
Sbjct: 61  YGDLYDHDSLVKAIKQVDVVISTVGFMQIADQTKIIAAIKEAGNVKRFFPSEFGNDVDHV 120

Query: 133 HAVEPAKSM-FDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKII 192
           +AVEPAKS+ F  K  IRRAVEAEGIP+T+V  N F+GYFL    QPGAT PPRDK+ I 
Sbjct: 121 NAVEPAKSVAFAVKANIRRAVEAEGIPYTYVASNCFNGYFLPTLVQPGATTPPRDKVIIP 180

Query: 193 GDGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLE 252
           GDGNPK I+NKEEDIGTYTI+A++DPRTLNKILY+RP  NIYS NELV+LWE+KIG TLE
Sbjct: 181 GDGNPKAIFNKEEDIGTYTIKAVDDPRTLNKILYLRPSNNIYSFNELVALWEKKIGKTLE 240

Query: 253 RIYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYAT 312
           +IY+PEE++LK+IQEAP P+N  L I+H+ FVKGDHTNF+IEPSFGVEASELY +V+Y T
Sbjct: 241 KIYVPEEQILKDIQEAPIPINIFLGINHSVFVKGDHTNFEIEPSFGVEASELYPEVKYTT 300

Query: 313 VEEYLNQFV 321
           VEEYL+QFV
Sbjct: 301 VEEYLDQFV 309

BLAST of Tan0009200 vs. ExPASy Swiss-Prot
Match: P52578 (Isoflavone reductase homolog OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 1.8e-125
Identity = 217/308 (70.45%), Postives = 256/308 (83.12%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           MA K K+L IGGTGYIGKFIVEASAKAGH T+ LVRESTL+N  K+ +ID F+S GV F+
Sbjct: 1   MAGKSKILFIGGTGYIGKFIVEASAKAGHDTFVLVRESTLSNPTKTKLIDTFKSFGVTFV 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
            GD++D+ESLVKAIK VDVVISTVG ALLA Q K+I+AIK+AGNVKRFFPSEFGNDVDRV
Sbjct: 61  HGDLYDHESLVKAIKQVDVVISTVGHALLADQVKLIAAIKEAGNVKRFFPSEFGNDVDRV 120

Query: 133 HAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 192
           HAVEPAK+ F+ K +IRR VEAEGIP T+V   FF GY L N  QPGA  PP DK+ I+G
Sbjct: 121 HAVEPAKAAFNTKAQIRRVVEAEGIPFTYVATFFFAGYSLPNLAQPGAAGPPNDKVVILG 180

Query: 193 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 252
            GN K ++NKEEDIGTYTI A++DP+TLNKILYI+PP NI + NELVSLWE+K G  LER
Sbjct: 181 HGNTKAVFNKEEDIGTYTINAVDDPKTLNKILYIKPPHNIITLNELVSLWEKKTGKNLER 240

Query: 253 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 312
           +Y+PEE++LKNIQEA  P+N  L+I HTAFVKGDHTNF+IEPSFGVEASE+Y DV+Y  +
Sbjct: 241 LYVPEEQVLKNIQEASVPMNVGLSIYHTAFVKGDHTNFEIEPSFGVEASEVYPDVKYTPI 300

Query: 313 EEYLNQFV 321
           +E LNQ+V
Sbjct: 301 DEILNQYV 308

BLAST of Tan0009200 vs. NCBI nr
Match: XP_022947777.1 (isoflavone reductase-like protein [Cucurbita moschata] >KAG6605074.1 Phenylcoumaran benzylic ether reductase Pyrc5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 582.8 bits (1501), Expect = 1.8e-162
Identity = 290/308 (94.16%), Postives = 300/308 (97.40%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           MAQKLKVLIIGGTGYIGKFIVEASAKAGH TYALVRESTLA+SAKS IIDNFRSLGVNFL
Sbjct: 1   MAQKLKVLIIGGTGYIGKFIVEASAKAGHPTYALVRESTLADSAKSSIIDNFRSLGVNFL 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
            GDIFDNESLVKAIK VDVVISTVGRALLA+Q+KIISAIKQAGNVKRF PSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVKAIKQVDVVISTVGRALLANQDKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 133 HAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 192
           HAVEPAKSMF AK EIRRA+EAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG
Sbjct: 121 HAVEPAKSMFAAKAEIRRAIEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 180

Query: 193 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 252
           DG+PKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWE+KIG+TLER
Sbjct: 181 DGSPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWEKKIGTTLER 240

Query: 253 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 312
           IY+PEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNF+IEPSFGVEASELYSDV+Y TV
Sbjct: 241 IYVPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFEIEPSFGVEASELYSDVKYTTV 300

Query: 313 EEYLNQFV 321
           EEYLNQFV
Sbjct: 301 EEYLNQFV 308

BLAST of Tan0009200 vs. NCBI nr
Match: KAG7035082.1 (hypothetical protein SDJN02_01877, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 580.9 bits (1496), Expect = 6.9e-162
Identity = 288/308 (93.51%), Postives = 300/308 (97.40%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           MAQKLKVLIIGGTGYIGKFIVEASAKAGH TYALVRESTLA+SAKS IIDNFRSLGVNFL
Sbjct: 1   MAQKLKVLIIGGTGYIGKFIVEASAKAGHPTYALVRESTLADSAKSSIIDNFRSLGVNFL 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
            GDIFDNESLVKAIK VDVVISTVGRALLA+Q+KIISAIKQAGNVKRF PSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVKAIKQVDVVISTVGRALLANQDKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 133 HAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 192
           HAVEPAKSMF AK EIRRA+EAEGIPHTFV+CNFFDGYFLRNFCQPGATDPPRDKIKIIG
Sbjct: 121 HAVEPAKSMFAAKAEIRRAIEAEGIPHTFVICNFFDGYFLRNFCQPGATDPPRDKIKIIG 180

Query: 193 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 252
           DG+PKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWE+KIG+TLE+
Sbjct: 181 DGSPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWEKKIGTTLEQ 240

Query: 253 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 312
           IY+PEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNF+IEPSFGVEASELYSDV+Y TV
Sbjct: 241 IYVPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFEIEPSFGVEASELYSDVKYTTV 300

Query: 313 EEYLNQFV 321
           EEYLNQFV
Sbjct: 301 EEYLNQFV 308

BLAST of Tan0009200 vs. NCBI nr
Match: XP_023006885.1 (isoflavone reductase-like protein [Cucurbita maxima])

HSP 1 Score: 580.1 bits (1494), Expect = 1.2e-161
Identity = 289/308 (93.83%), Postives = 298/308 (96.75%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           MAQKLKVLIIGGTGYIGKFIVEASAKAGH TY LVRESTLA+SAKS IIDNFRSLGVNFL
Sbjct: 1   MAQKLKVLIIGGTGYIGKFIVEASAKAGHPTYVLVRESTLADSAKSSIIDNFRSLGVNFL 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
            GDIFDNESLVKAIK VDVVISTVGRALLA+++KIISAIKQAGNVKRF PSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVKAIKQVDVVISTVGRALLANEDKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 133 HAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 192
           HAVEPAKSMF AK EIRRA+EAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG
Sbjct: 121 HAVEPAKSMFAAKAEIRRAIEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 180

Query: 193 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 252
           DG+PKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWE+KIG TLER
Sbjct: 181 DGSPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWEKKIGKTLER 240

Query: 253 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 312
           IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNF+IEPSFGVEASELYSDV+Y TV
Sbjct: 241 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFEIEPSFGVEASELYSDVKYTTV 300

Query: 313 EEYLNQFV 321
           EEYLNQFV
Sbjct: 301 EEYLNQFV 308

BLAST of Tan0009200 vs. NCBI nr
Match: XP_023533158.1 (isoflavone reductase homolog PCBER-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 578.2 bits (1489), Expect = 4.5e-161
Identity = 287/308 (93.18%), Postives = 299/308 (97.08%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           MAQKLKVLIIGGTGYIGKFIVEASAKAGH TYALVRESTLA+SAKS IIDNFRSLGVNFL
Sbjct: 1   MAQKLKVLIIGGTGYIGKFIVEASAKAGHPTYALVRESTLADSAKSSIIDNFRSLGVNFL 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
            GDIFDNESLVKAIK VDVVISTVGR LLA+Q+KIISAIKQAGNVKRF PSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVKAIKQVDVVISTVGRTLLANQDKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 133 HAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 192
           HAVEPAKSMF AK EIRRA+EAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRD++KIIG
Sbjct: 121 HAVEPAKSMFAAKAEIRRAIEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDRVKIIG 180

Query: 193 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 252
           DG+PKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWE+KIG+TLER
Sbjct: 181 DGSPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWEKKIGTTLER 240

Query: 253 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 312
           IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNF+IEPSFGVEASELYSDV+Y TV
Sbjct: 241 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFEIEPSFGVEASELYSDVKYTTV 300

Query: 313 EEYLNQFV 321
           EEYL+QFV
Sbjct: 301 EEYLDQFV 308

BLAST of Tan0009200 vs. NCBI nr
Match: XP_038900750.1 (phenylcoumaran benzylic ether reductase POP1-like [Benincasa hispida])

HSP 1 Score: 531.2 bits (1367), Expect = 6.3e-147
Identity = 263/308 (85.39%), Postives = 284/308 (92.21%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           M Q+ KVLIIGGTGYIGKF+VEASAKAGH T ALVRESTLA+S KS IIDNFRSLGVN +
Sbjct: 1   MPQQPKVLIIGGTGYIGKFLVEASAKAGHPTCALVRESTLADSGKSSIIDNFRSLGVNLV 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
            GDIFDNESLV+AI+ VDVVISTV RALL+HQ+KIISAIKQAGNVKRFFPSEFGNDVDRV
Sbjct: 61  TGDIFDNESLVRAIQQVDVVISTVSRALLSHQDKIISAIKQAGNVKRFFPSEFGNDVDRV 120

Query: 133 HAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 192
           HAVEPAK MF AK EIRRA+EAEGIPHTFVV NFF GY LRNF QPGATDPPRDKIKI+G
Sbjct: 121 HAVEPAKRMFAAKAEIRRAIEAEGIPHTFVVSNFFGGYHLRNFSQPGATDPPRDKIKILG 180

Query: 193 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 252
           DG+PKV+YNKEEDIGTYTIRAI+DPRTLNKILYIRPPANIYSTN+LVSLWE+KIG++LER
Sbjct: 181 DGSPKVVYNKEEDIGTYTIRAIDDPRTLNKILYIRPPANIYSTNDLVSLWEKKIGNSLER 240

Query: 253 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 312
            Y+ EEEL+KNIQE PFPLNT LAISH+AFVKGDHTNFDIEP+ GVEASELY DVQY TV
Sbjct: 241 TYVSEEELVKNIQETPFPLNTGLAISHSAFVKGDHTNFDIEPAVGVEASELYPDVQYTTV 300

Query: 313 EEYLNQFV 321
           EEYLNQFV
Sbjct: 301 EEYLNQFV 308

BLAST of Tan0009200 vs. ExPASy TrEMBL
Match: A0A6J1G7E0 (isoflavone reductase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111451536 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 8.8e-163
Identity = 290/308 (94.16%), Postives = 300/308 (97.40%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           MAQKLKVLIIGGTGYIGKFIVEASAKAGH TYALVRESTLA+SAKS IIDNFRSLGVNFL
Sbjct: 1   MAQKLKVLIIGGTGYIGKFIVEASAKAGHPTYALVRESTLADSAKSSIIDNFRSLGVNFL 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
            GDIFDNESLVKAIK VDVVISTVGRALLA+Q+KIISAIKQAGNVKRF PSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVKAIKQVDVVISTVGRALLANQDKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 133 HAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 192
           HAVEPAKSMF AK EIRRA+EAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG
Sbjct: 121 HAVEPAKSMFAAKAEIRRAIEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 180

Query: 193 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 252
           DG+PKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWE+KIG+TLER
Sbjct: 181 DGSPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWEKKIGTTLER 240

Query: 253 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 312
           IY+PEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNF+IEPSFGVEASELYSDV+Y TV
Sbjct: 241 IYVPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFEIEPSFGVEASELYSDVKYTTV 300

Query: 313 EEYLNQFV 321
           EEYLNQFV
Sbjct: 301 EEYLNQFV 308

BLAST of Tan0009200 vs. ExPASy TrEMBL
Match: A0A6J1L1G1 (isoflavone reductase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111499544 PE=4 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 5.7e-162
Identity = 289/308 (93.83%), Postives = 298/308 (96.75%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           MAQKLKVLIIGGTGYIGKFIVEASAKAGH TY LVRESTLA+SAKS IIDNFRSLGVNFL
Sbjct: 1   MAQKLKVLIIGGTGYIGKFIVEASAKAGHPTYVLVRESTLADSAKSSIIDNFRSLGVNFL 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
            GDIFDNESLVKAIK VDVVISTVGRALLA+++KIISAIKQAGNVKRF PSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVKAIKQVDVVISTVGRALLANEDKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 133 HAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 192
           HAVEPAKSMF AK EIRRA+EAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG
Sbjct: 121 HAVEPAKSMFAAKAEIRRAIEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 180

Query: 193 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 252
           DG+PKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWE+KIG TLER
Sbjct: 181 DGSPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWEKKIGKTLER 240

Query: 253 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 312
           IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNF+IEPSFGVEASELYSDV+Y TV
Sbjct: 241 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFEIEPSFGVEASELYSDVKYTTV 300

Query: 313 EEYLNQFV 321
           EEYLNQFV
Sbjct: 301 EEYLNQFV 308

BLAST of Tan0009200 vs. ExPASy TrEMBL
Match: A0A5A7TVM0 (Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00380 PE=4 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 2.2e-145
Identity = 258/308 (83.77%), Postives = 281/308 (91.23%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           MAQKLKVLIIGGTGYIGKF+VEASA+AGH T+ LVR+STL++SAKS IID+FR+LGVNF+
Sbjct: 1   MAQKLKVLIIGGTGYIGKFVVEASAQAGHPTFVLVRQSTLSDSAKSSIIDSFRNLGVNFV 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
            GDIFDNESLV+AI+ VDVVISTVGR LL+HQ+ IISAIKQAGN+KRFFPSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQQNIISAIKQAGNIKRFFPSEFGNDVDRV 120

Query: 133 HAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 192
           HAVEPAKSMF  KVEIRR VEAEGIPHTFVV NFFDGY+LRNF QPGAT+PPRDKIKI G
Sbjct: 121 HAVEPAKSMFARKVEIRRVVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 180

Query: 193 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 252
           DGN KVIYNKEEDIGTYTIRAI+DPRT NKILYIRPPANIYSTNELVSLWE+KI   LE+
Sbjct: 181 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKICRILEK 240

Query: 253 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 312
            Y+ EEEL+KNIQE P PLNT LAISH+AFVKGDHTNFDIEPS GVEASELY DV Y TV
Sbjct: 241 TYVSEEELVKNIQETPVPLNTALAISHSAFVKGDHTNFDIEPSIGVEASELYPDVHYTTV 300

Query: 313 EEYLNQFV 321
           E+YLNQFV
Sbjct: 301 EDYLNQFV 308

BLAST of Tan0009200 vs. ExPASy TrEMBL
Match: A0A0A0LL65 (NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G248135 PE=4 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 2.2e-145
Identity = 259/308 (84.09%), Postives = 282/308 (91.56%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           MAQ LKVL+IG TGYIGKF+VEASA+AGH T+ALVR+STLA+SAKS II NFR+LGVNF+
Sbjct: 1   MAQNLKVLVIGATGYIGKFVVEASAQAGHPTFALVRQSTLADSAKSSIIHNFRNLGVNFV 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
            GDIFDNESLV+AI+ VDVVISTVGR LL+HQEKIISAIKQAGNVKRF PSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQEKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 133 HAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 192
           HAVEPAKSMF +KVEIRRAVEAEGIPHTFVV NFFDGY+LRNF QPGAT+PPRDKIKI G
Sbjct: 121 HAVEPAKSMFASKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 180

Query: 193 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 252
           DGN KVIYNKEEDIGTYTIRAI+DPRT NKILYIRPPANIYSTNELVSLWE+KIG  LER
Sbjct: 181 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKIGRILER 240

Query: 253 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 312
            Y+ EEEL+KNIQE P PL+T LAISH+AFVKGDHTNF+IEPS GVEASELY +V Y TV
Sbjct: 241 TYVSEEELVKNIQETPVPLSTALAISHSAFVKGDHTNFEIEPSIGVEASELYPNVHYTTV 300

Query: 313 EEYLNQFV 321
           E+YLNQFV
Sbjct: 301 EDYLNQFV 308

BLAST of Tan0009200 vs. ExPASy TrEMBL
Match: A0A1S3CET9 (isoflavone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103499681 PE=4 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 2.2e-145
Identity = 258/308 (83.77%), Postives = 281/308 (91.23%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           MAQKLKVLIIGGTGYIGKF+VEASA+AGH T+ LVR+STL++SAKS IID+FR+LGVNF+
Sbjct: 1   MAQKLKVLIIGGTGYIGKFVVEASAQAGHPTFVLVRQSTLSDSAKSSIIDSFRNLGVNFV 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
            GDIFDNESLV+AI+ VDVVISTVGR LL+HQ+ IISAIKQAGN+KRFFPSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQQNIISAIKQAGNIKRFFPSEFGNDVDRV 120

Query: 133 HAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 192
           HAVEPAKSMF  KVEIRR VEAEGIPHTFVV NFFDGY+LRNF QPGAT+PPRDKIKI G
Sbjct: 121 HAVEPAKSMFARKVEIRRVVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 180

Query: 193 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 252
           DGN KVIYNKEEDIGTYTIRAI+DPRT NKILYIRPPANIYSTNELVSLWE+KI   LE+
Sbjct: 181 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKICRILEK 240

Query: 253 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 312
            Y+ EEEL+KNIQE P PLNT LAISH+AFVKGDHTNFDIEPS GVEASELY DV Y TV
Sbjct: 241 TYVSEEELVKNIQETPVPLNTALAISHSAFVKGDHTNFDIEPSIGVEASELYPDVHYTTV 300

Query: 313 EEYLNQFV 321
           E+YLNQFV
Sbjct: 301 EDYLNQFV 308

BLAST of Tan0009200 vs. TAIR 10
Match: AT4G39230.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 442.6 bits (1137), Expect = 2.7e-124
Identity = 212/308 (68.83%), Postives = 252/308 (81.82%), Query Frame = 0

Query: 13  MAQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFL 72
           M  K K+L IGGTGYIGK+IVEASA++GH T  LVR STL + ++S  I+NF++LGV FL
Sbjct: 1   MTSKSKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFL 60

Query: 73  IGDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRV 132
           +GD+ D+ SLV +IK  DVVISTVG +LL HQ KIISAIK+AGNVKRFFPSEFGNDVDRV
Sbjct: 61  LGDLDDHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRV 120

Query: 133 HAVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 192
             VEPAKS +  K +IRR +EAEGIP+T+V CNFF GYFL    QPGAT  PRDK+ ++G
Sbjct: 121 FTVEPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLG 180

Query: 193 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 252
           DGNPK ++NKEEDIGTYTI A++DPRTLNKILYIRPP N YS N+LVSLWE KIG TLER
Sbjct: 181 DGNPKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLER 240

Query: 253 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 312
           IY+PEE+LLK I E+  PLN +L++ H  FVKG HT+F+IEPSFGVEASELY DV+Y TV
Sbjct: 241 IYVPEEQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTV 300

Query: 313 EEYLNQFV 321
           +E LNQ+V
Sbjct: 301 DEILNQYV 308

BLAST of Tan0009200 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 403.7 bits (1036), Expect = 1.4e-112
Identity = 198/307 (64.50%), Postives = 243/307 (79.15%), Query Frame = 0

Query: 15  QKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFLIG 74
           +K K+L+IGGTGYIGKF+VEASAKAGHST+ALVRE+TL++  K   + +F+ LGV  L G
Sbjct: 4   EKSKILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHG 63

Query: 75  DIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRVHA 134
           D+ D+ESLVKAIK VDVVISTVG   +  Q KIISAIK+AGNVKRF PSEFG DVDR  A
Sbjct: 64  DLNDHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRTSA 123

Query: 135 VEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQ--PGATDPPRDKIKIIG 194
           VEPAKS F  K++IRR +EAEGIP+T+ V   F GY+L    Q  PG T PPRDK+ I+G
Sbjct: 124 VEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILG 183

Query: 195 DGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLER 254
           DGN K + NKEEDI  YTI+A++DPRTLNKILYI+P  N  S NE+V+LWE+KIG +LE+
Sbjct: 184 DGNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKSLEK 243

Query: 255 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATV 314
            ++PEE+LLK+IQE+P P+N VL+I+H  FV GD TN  IEPSFGVEASELY DV+Y +V
Sbjct: 244 THLPEEQLLKSIQESPIPINVVLSINHAVFVNGD-TNISIEPSFGVEASELYPDVKYTSV 303

Query: 315 EEYLNQF 320
           +EYL+ F
Sbjct: 304 DEYLSYF 309

BLAST of Tan0009200 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 395.2 bits (1014), Expect = 5.0e-110
Identity = 189/308 (61.36%), Postives = 239/308 (77.60%), Query Frame = 0

Query: 14  AQKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFLI 73
           ++K K+L+IGGTG+IGK I+EAS KAGHST ALVRE++L++  K   + NF+  GV  L 
Sbjct: 3   SEKSKILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLH 62

Query: 74  GDIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRVH 133
           GD+ D+ESLVKAIK  DVVISTVG   +  Q KIISAIK+AGNVKRF PSEFG DVD+  
Sbjct: 63  GDLNDHESLVKAIKQADVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDKSS 122

Query: 134 AVEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQ--PGATDPPRDKIKII 193
           AVEPAKS F  K++ RR +EAEGIP+T++V N+F GY+L    Q  PG T PPRDK+KI 
Sbjct: 123 AVEPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVKIF 182

Query: 194 GDGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLE 253
           GDGN K + NKEEDI  YTI+A++DPRTLNK LYI PP N  S NE+V+LWE+KIG ++E
Sbjct: 183 GDGNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKSVE 242

Query: 254 RIYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYAT 313
           +IY+ EE++ K+IQE+P P N +L+I+H  FVKGD TNF IEPSFG EASELY D++Y +
Sbjct: 243 KIYMSEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDIKYTS 302

Query: 314 VEEYLNQF 320
           ++EYL+ F
Sbjct: 303 IDEYLSYF 310

BLAST of Tan0009200 vs. TAIR 10
Match: AT1G75300.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 360.5 bits (924), Expect = 1.4e-99
Identity = 183/321 (57.01%), Postives = 236/321 (73.52%), Query Frame = 0

Query: 15  QKLKVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFLIG 74
           +K K+L+IGGTGY+G+FIVE SAKAG+ T+ALVRE++L++  KS  I +F+ LGV  L G
Sbjct: 4   EKSKILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHG 63

Query: 75  DIFDNESLVKAIKHVDVVISTVGRALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRVHA 134
           D+ D+ESLVKAIK VDVVIST+G   +  Q KIISAIK+AGNVKRF P+EFG DV+R  A
Sbjct: 64  DLNDHESLVKAIKQVDVVISTIGHKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVERTSA 123

Query: 135 VEPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFLRNFCQ----------------P 194
           VEPAKS+F  KV+IRRA+EAEGIP+T+VV N   G++LR   Q                 
Sbjct: 124 VEPAKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAIIFG 183

Query: 195 GATDPPRDKIKIIGDGNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNEL 254
               PPRDK+ I+GDGN KV+ NKEED+  Y I+A++D RTLNK LYI PP NI S NE+
Sbjct: 184 DKNVPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSMNEM 243

Query: 255 VSLWERKIGSTLERIYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGV 314
           V+LWE+KIG +LE+ +I EE++LK+IQ    P++   +I+H  FVKGD T+F IEP FG 
Sbjct: 244 VTLWEKKIGKSLEKTHISEEQILKSIQ---VPIDVFKSINHAVFVKGDQTSFTIEPWFGE 303

Query: 315 EASELYSDVQYATVEEYLNQF 320
           EAS LY DV+Y +++EYL+QF
Sbjct: 304 EASVLYPDVKYTSIDEYLSQF 321

BLAST of Tan0009200 vs. TAIR 10
Match: AT1G19540.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 342.4 bits (877), Expect = 3.8e-94
Identity = 172/307 (56.03%), Postives = 222/307 (72.31%), Query Frame = 0

Query: 18  KVLIIGGTGYIGKFIVEASAKAGHSTYALVRESTLANSAKSPIIDNFRSLGVNFLIGDIF 77
           K+L+IG TG IGK +VE SAK+GH+T+ALVRE++L++  K+ +++ F+ LGV  L G + 
Sbjct: 4   KILVIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYGSLS 63

Query: 78  DNESLVKAIKHVDVVISTVGR--ALLAHQEKIISAIKQAGNVKRFFPSEFGNDVDRVHAV 137
           D ESLVKAIK VDVVIS VGR    + +Q  II AIK++GNVKRF PSEFGNDVDR  A+
Sbjct: 64  DKESLVKAIKQVDVVISAVGRFQTEILNQTNIIDAIKESGNVKRFLPSEFGNDVDRTVAI 123

Query: 138 EPAKSMFDAKVEIRRAVEAEGIPHTFVVCNFFDGYFL--RNFCQPGATDPPRDKIKIIGD 197
           EP  S F  K +IRRA+EA  IP+T+VV   F G F+     C      PPRDK+ I   
Sbjct: 124 EPTLSEFITKAQIRRAIEAAKIPYTYVVSGCFAGLFVPCLGQCHLRLRSPPRDKVSIYDT 183

Query: 198 GNPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWERKIGSTLERI 257
           GN K I N EEDI  YT++A++DPRTLNKILYI PP  I S N++V LWE KIG TLE+ 
Sbjct: 184 GNGKAIVNTEEDIVAYTLKAVDDPRTLNKILYIHPPNYIVSQNDMVGLWEEKIGKTLEKT 243

Query: 258 YIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFDIEPSFGVEASELYSDVQYATVE 317
           Y+ EEELLK IQE+  P++ ++ + HT  VK D T+F I+PSFGVEASELY +V+Y +V+
Sbjct: 244 YVSEEELLKTIQESKPPMDFLVGLIHTILVKSDFTSFTIDPSFGVEASELYPEVKYTSVD 303

Query: 318 EYLNQFV 321
           E+LN+F+
Sbjct: 304 EFLNRFI 310

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B9HRL72.4e-13375.58Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=P... [more]
O813557.0e-13374.35Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC... [more]
Q9FUW67.5e-12770.13Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6... [more]
B2WSN01.8e-12570.55Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1[more]
P525781.8e-12570.45Isoflavone reductase homolog OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022947777.11.8e-16294.16isoflavone reductase-like protein [Cucurbita moschata] >KAG6605074.1 Phenylcouma... [more]
KAG7035082.16.9e-16293.51hypothetical protein SDJN02_01877, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023006885.11.2e-16193.83isoflavone reductase-like protein [Cucurbita maxima][more]
XP_023533158.14.5e-16193.18isoflavone reductase homolog PCBER-like [Cucurbita pepo subsp. pepo][more]
XP_038900750.16.3e-14785.39phenylcoumaran benzylic ether reductase POP1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1G7E08.8e-16394.16isoflavone reductase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111451536 ... [more]
A0A6J1L1G15.7e-16293.83isoflavone reductase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111499544 PE... [more]
A0A5A7TVM02.2e-14583.77Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A0A0LL652.2e-14584.09NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G248135 PE=4 S... [more]
A0A1S3CET92.2e-14583.77isoflavone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103499681 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G39230.12.7e-12468.83NmrA-like negative transcriptional regulator family protein [more]
AT1G75280.11.4e-11264.50NmrA-like negative transcriptional regulator family protein [more]
AT1G75290.15.0e-11061.36NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G75300.11.4e-9957.01NmrA-like negative transcriptional regulator family protein [more]
AT1G19540.13.8e-9456.03NmrA-like negative transcriptional regulator family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008030NmrA-like domainPFAMPF05368NmrAcoord: 19..249
e-value: 3.8E-79
score: 265.5
NoneNo IPR availableGENE3D3.90.25.10coord: 166..316
e-value: 2.9E-137
score: 458.7
NoneNo IPR availableGENE3D3.40.50.720coord: 18..306
e-value: 2.9E-137
score: 458.7
NoneNo IPR availablePANTHERPTHR43349PINORESINOL REDUCTASE-RELATEDcoord: 13..320
NoneNo IPR availablePANTHERPTHR43349:SF35PHENYLCOUMARAN BENZYLIC ETHER REDUCTASE 1coord: 13..320
NoneNo IPR availableCDDcd05259PCBER_SDR_acoord: 18..315
e-value: 1.57932E-85
score: 256.846
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 18..288

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0009200.1Tan0009200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009807 lignan biosynthetic process
molecular_function GO:0016491 oxidoreductase activity