Tan0009133 (gene) Snake gourd v1

Overview


Name	Tan0009133
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	ERAD-associated E3 ubiquitin-protein ligase HRD1-like
Location	LG02: 18806659 .. 18807554 (-)
RNA-Seq Expression	Tan0009133
Synteny	Tan0009133

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACCAAAATCAAAAAAGCAAAAATAAAATATACAAATTTGGAAACACTTACCAAATAAATATTCTTCAATCTCCATCAATTTTCCATTCATCAAATCCCTAAACAATAATTTCAATCTGTTCAATGGATTATCACATCTCTCGATACTCGAATCTCCTTGTCGAAGTAGCTACCGATCTCCCTGTTCCAAGATCCACAAAAATCCCCAAAACTCCATCGCCAATCGAACTCATCGGCCTGCTCTTCACCCTCCGTCACCGTCGCAGCCGCTTCGACGTATCGGAGAACGGCGAGCGGCAGCTTGTATGGCAGCAAGAAGATCTGAAAATTCCACTCTGCGACCACCAATTCCGGTCCGAAATCTTCAACCGAGGCGACGATACTCTGTTCCACTACCTGTCCTGCAGTTTCCAGAGCTTAGAACAAGGAATCGACGCGGAATCGGTAGCGGAAGCCCTAATCGAGCGGTGGAGTGAGATCAGGGAGGAAGTGAATTCGAGAAGCACGGAGAGAGTGTTTTCGATGACGGTGAATTTGACGCTGTGGTTCATCGAGGCTCACCATGAGATTGGCGGCGTCGTGGCTGGAGAAAATTGGGGAATGATTCCGGCGTGCGGCGAGGCGGTAACGAAGATGTTGAAGAGAATTGAGATGGGAGAAGATGATGGAGTTTGTGTGATTTGTTTGGAGGAATTGAAGAAGAAGAATGGAGTGTGGGGTTTGCAAATGCCTTGTTCACATTTGTTTCATCAAAATTGCATTGAAGAGTGGTTGAAGAAAAGCCATTGTTGTCCCATTTGTAGATTTCAAATGCCTATTCCTCAATTATTTTAGTTTTTTCTTTATCTAGTTATATATATATATATATACCCTTATACCCTCATTTTATTAAACCC

mRNA sequence

AACCAAAATCAAAAAAGCAAAAATAAAATATACAAATTTGGAAACACTTACCAAATAAATATTCTTCAATCTCCATCAATTTTCCATTCATCAAATCCCTAAACAATAATTTCAATCTGTTCAATGGATTATCACATCTCTCGATACTCGAATCTCCTTGTCGAAGTAGCTACCGATCTCCCTGTTCCAAGATCCACAAAAATCCCCAAAACTCCATCGCCAATCGAACTCATCGGCCTGCTCTTCACCCTCCGTCACCGTCGCAGCCGCTTCGACGTATCGGAGAACGGCGAGCGGCAGCTTGTATGGCAGCAAGAAGATCTGAAAATTCCACTCTGCGACCACCAATTCCGGTCCGAAATCTTCAACCGAGGCGACGATACTCTGTTCCACTACCTGTCCTGCAGTTTCCAGAGCTTAGAACAAGGAATCGACGCGGAATCGGTAGCGGAAGCCCTAATCGAGCGGTGGAGTGAGATCAGGGAGGAAGTGAATTCGAGAAGCACGGAGAGAGTGTTTTCGATGACGGTGAATTTGACGCTGTGGTTCATCGAGGCTCACCATGAGATTGGCGGCGTCGTGGCTGGAGAAAATTGGGGAATGATTCCGGCGTGCGGCGAGGCGGTAACGAAGATGTTGAAGAGAATTGAGATGGGAGAAGATGATGGAGTTTGTGTGATTTGTTTGGAGGAATTGAAGAAGAAGAATGGAGTGTGGGGTTTGCAAATGCCTTGTTCACATTTGTTTCATCAAAATTGCATTGAAGAGTGGTTGAAGAAAAGCCATTGTTGTCCCATTTGTAGATTTCAAATGCCTATTCCTCAATTATTTTAGTTTTTTCTTTATCTAGTTATATATATATATATATACCCTTATACCCTCATTTTATTAAACCC

Coding sequence (CDS)

ATGGATTATCACATCTCTCGATACTCGAATCTCCTTGTCGAAGTAGCTACCGATCTCCCTGTTCCAAGATCCACAAAAATCCCCAAAACTCCATCGCCAATCGAACTCATCGGCCTGCTCTTCACCCTCCGTCACCGTCGCAGCCGCTTCGACGTATCGGAGAACGGCGAGCGGCAGCTTGTATGGCAGCAAGAAGATCTGAAAATTCCACTCTGCGACCACCAATTCCGGTCCGAAATCTTCAACCGAGGCGACGATACTCTGTTCCACTACCTGTCCTGCAGTTTCCAGAGCTTAGAACAAGGAATCGACGCGGAATCGGTAGCGGAAGCCCTAATCGAGCGGTGGAGTGAGATCAGGGAGGAAGTGAATTCGAGAAGCACGGAGAGAGTGTTTTCGATGACGGTGAATTTGACGCTGTGGTTCATCGAGGCTCACCATGAGATTGGCGGCGTCGTGGCTGGAGAAAATTGGGGAATGATTCCGGCGTGCGGCGAGGCGGTAACGAAGATGTTGAAGAGAATTGAGATGGGAGAAGATGATGGAGTTTGTGTGATTTGTTTGGAGGAATTGAAGAAGAAGAATGGAGTGTGGGGTTTGCAAATGCCTTGTTCACATTTGTTTCATCAAAATTGCATTGAAGAGTGGTTGAAGAAAAGCCATTGTTGTCCCATTTGTAGATTTCAAATGCCTATTCCTCAATTATTTTAG

Protein sequence

MDYHISRYSNLLVEVATDLPVPRSTKIPKTPSPIELIGLLFTLRHRRSRFDVSENGERQLVWQQEDLKIPLCDHQFRSEIFNRGDDTLFHYLSCSFQSLEQGIDAESVAEALIERWSEIREEVNSRSTERVFSMTVNLTLWFIEAHHEIGGVVAGENWGMIPACGEAVTKMLKRIEMGEDDGVCVICLEELKKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICRFQMPIPQLF

Homology

[Close]

BLAST of Tan0009133 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.5e-11
Identity = 30/71 (42.25%), Postives = 42/71 (59.15%), Query Frame = 0


Query: 162 PACGEAVTKMLKRIEMGEDDGVCVICLEELKKKNGVWGLQMPCSHLFHQNCIEEWLKKSH 221
           P   +A    +  +E+   +G CVICLEE K +  V   +MPC H FH  CIE+WL    
Sbjct: 91  PPASKASIDAMPIVEIDGCEGECVICLEEWKSEETV--KEMPCKHRFHGGCIEKWLGFHG 150

Query: 222 CCPICRFQMPI 233
            CP+CR++MP+
Sbjct: 151 SCPVCRYEMPV 159

[Close]

BLAST of Tan0009133 vs. ExPASy Swiss-Prot
Match: Q9CY62 (E3 ubiquitin-protein ligase RNF181 OS=Mus musculus OX=10090 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 7.2e-10
Identity = 30/72 (41.67%), Postives = 43/72 (59.72%), Query Frame = 0


Query: 162 PACGEAVTKMLKR--IEMGEDDGVCVICLEELKKKNGVWGLQMPCSHLFHQNCIEEWLKK 221
           P   +AV + L R  I   + D  C +CL E + +  V  ++MPC HLFH NCI  WL K
Sbjct: 64  PPAAKAVVESLPRTVISSAKADLKCPVCLLEFEAEETV--IEMPCHHLFHSNCILPWLSK 123

Query: 222 SHCCPICRFQMP 232
           ++ CP+CR ++P
Sbjct: 124 TNSCPLCRHELP 133

[Close]

BLAST of Tan0009133 vs. ExPASy Swiss-Prot
Match: Q6AXU4 (E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.2e-09
Identity = 29/72 (40.28%), Postives = 44/72 (61.11%), Query Frame = 0


Query: 162 PACGEAVTKMLKR--IEMGEDDGVCVICLEELKKKNGVWGLQMPCSHLFHQNCIEEWLKK 221
           P   +AV + L R  I   + +  C +CL E +++  V  ++MPC HLFH NCI  WL K
Sbjct: 64  PPAAKAVVESLPRTVIRSSKAELKCPVCLLEFEEEETV--IEMPCHHLFHSNCILPWLSK 123

Query: 222 SHCCPICRFQMP 232
           ++ CP+CR ++P
Sbjct: 124 TNSCPLCRHELP 133

[Close]

BLAST of Tan0009133 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.6e-09
Identity = 26/64 (40.62%), Postives = 42/64 (65.62%), Query Frame = 0


Query: 168 VTKMLKRIEMGEDDGVCVICLEELKKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICR 227
           VTK + + EM +    C +C++E +  + V   QMPC H+FHQ+C+  WL+  + CP+CR
Sbjct: 204 VTKDMLKSEMNQ----CAVCMDEFEDGSDV--KQMPCKHVFHQDCLLPWLELHNSCPVCR 261

Query: 228 FQMP 232
           F++P
Sbjct: 264 FELP 261

[Close]

BLAST of Tan0009133 vs. ExPASy Swiss-Prot
Match: Q8GXF8 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SGR9 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.6e-09
Identity = 26/53 (49.06%), Postives = 32/53 (60.38%), Query Frame = 0


Query: 184 CVICLEELKKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICRFQMPIPQLF 237
           CVIC EE+ +   V   +MPC H FH  CI  WL K + CP CRFQ+P   +F
Sbjct: 214 CVICKEEMSEGRDV--CEMPCQHFFHWKCILPWLSKKNTCPFCRFQLPTDDVF 264

[Close]

BLAST of Tan0009133 vs. NCBI nr
Match: KAG6571896.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 323.6 bits (828), Expect = 1.5e-84
Identity = 158/236 (66.95%), Postives = 192/236 (81.36%), Query Frame = 0


Query: 1   MDYHISRYSNLLVEVATDLPVPRSTKIPKTPSPIELIGLLFTLRHRRSRFDVSENGERQL 60
           M++ ISRY NL+VEV TDLP P  ++IPK PS IE IGL+FTLR RRS F+VSENGER+L
Sbjct: 1   MEHQISRYENLVVEVLTDLPTP--SRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRL 60

Query: 61  VWQQEDLKIPLCDHQFRSEIFNRGDDTLFHYLSCSFQSLEQGIDAESVAEALIERWSEIR 120
           + Q+E LKIPLCD+QFR+EIFNR DD+LFH LSCSFQ+ +  IDAE VA+ALIERW EIR
Sbjct: 61  IQQRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIR 120

Query: 121 EEVNSRSTERVFSMTVNLTLWFIEAHHEIGGVVAGENWGMIPACGEAVTKMLKRIEMGED 180
           +E NSRS++RVF MTVNLT+W I A +EIG    G+  GM+PACG AV KML+R+E  ++
Sbjct: 121 DEANSRSSDRVFPMTVNLTIWHIVARYEIGVGSGGDGCGMVPACGGAVGKMLRRLEREDE 180

Query: 181 -DGVCVICLEEL---KKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICRFQMPI 233
            DG+CVICL+EL   KKKNG +GLQMPC H+FH NCIE+WLK SH CPICRF++PI
Sbjct: 181 VDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 234

[Close]

BLAST of Tan0009133 vs. NCBI nr
Match: XP_022952293.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita moschata] >KAG7011584.1 HRD1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 322.8 bits (826), Expect = 2.5e-84
Identity = 157/236 (66.53%), Postives = 192/236 (81.36%), Query Frame = 0


Query: 1   MDYHISRYSNLLVEVATDLPVPRSTKIPKTPSPIELIGLLFTLRHRRSRFDVSENGERQL 60
           M++ ISRY NL+VEV TDLP P  ++IPK PS IE IGL+FTLR RRS F+VSENGER+L
Sbjct: 1   MEHQISRYENLVVEVLTDLPTP--SRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRL 60

Query: 61  VWQQEDLKIPLCDHQFRSEIFNRGDDTLFHYLSCSFQSLEQGIDAESVAEALIERWSEIR 120
           + Q+E LKIPLCD+QFR+EIFNR DD+LFH LSCSFQ+ +  IDAE VA+ALIERW EIR
Sbjct: 61  IQQRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIR 120

Query: 121 EEVNSRSTERVFSMTVNLTLWFIEAHHEIGGVVAGENWGMIPACGEAVTKMLKRIEMGED 180
           +E NSRS++RVF MTVNLT+W I A +EIG    G+  GM+PACG AV KML+R++  ++
Sbjct: 121 DEANSRSSDRVFPMTVNLTMWHIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDE 180

Query: 181 -DGVCVICLEEL---KKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICRFQMPI 233
            DG+CVICL+EL   KKKNG +GLQMPC H+FH NCIE+WLK SH CPICRF++PI
Sbjct: 181 VDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 234

[Close]

BLAST of Tan0009133 vs. NCBI nr
Match: XP_022972455.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita maxima])

HSP 1 Score: 320.9 bits (821), Expect = 9.5e-84
Identity = 157/236 (66.53%), Postives = 192/236 (81.36%), Query Frame = 0


Query: 1   MDYHISRYSNLLVEVATDLPVPRSTKIPKTPSPIELIGLLFTLRHRRSRFDVSENGERQL 60
           M++ ISRY NL+VEV TDLP P  ++IPK PS IE IGL+FTLRHRRS F+VSE+GER+L
Sbjct: 1   MEHQISRYENLVVEVLTDLPTP--SRIPKHPSRIEHIGLIFTLRHRRSHFEVSESGERRL 60

Query: 61  VWQQEDLKIPLCDHQFRSEIFNRGDDTLFHYLSCSFQSLEQGIDAESVAEALIERWSEIR 120
           + Q+E LKIPLCD+QFR+EIFNR DD+LFH LS SFQ+ +  IDAE VA+ALIERW EIR
Sbjct: 61  IQQRETLKIPLCDNQFRAEIFNRDDDSLFHNLSSSFQNFKPEIDAEPVADALIERWIEIR 120

Query: 121 EEVNSRSTERVFSMTVNLTLWFIEAHHEIGGVVAGENWGMIPACGEAVTKMLKRIEMGED 180
           +E NSRS++RVF MTVNLT+W I A +EIG    G+  GM+PACG AV KML+R+E  ++
Sbjct: 121 DEANSRSSDRVFPMTVNLTMWHIVARYEIGVGSGGDGCGMVPACGGAVGKMLRRLEREDE 180

Query: 181 -DGVCVICLEEL---KKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICRFQMPI 233
            DG+CVICL+EL   KKKNG +GLQMPC H+FH NCIE+WLK SH CPICRF++PI
Sbjct: 181 VDGICVICLDELKKKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 234

[Close]

BLAST of Tan0009133 vs. NCBI nr
Match: XP_023511497.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 317.8 bits (813), Expect = 8.0e-83
Identity = 155/236 (65.68%), Postives = 191/236 (80.93%), Query Frame = 0


Query: 1   MDYHISRYSNLLVEVATDLPVPRSTKIPKTPSPIELIGLLFTLRHRRSRFDVSENGERQL 60
           M++ ISRY +L+VEV TDLP P  ++IPK PS IE IGL+FTLR RRS F+VSENGER+L
Sbjct: 1   MEHQISRYESLVVEVLTDLPTP--SRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRL 60

Query: 61  VWQQEDLKIPLCDHQFRSEIFNRGDDTLFHYLSCSFQSLEQGIDAESVAEALIERWSEIR 120
           + Q+E LKIPLCD+QFR+EIFNR DD+LFH LSCSFQ+ +  IDAE VA+ALIERW EIR
Sbjct: 61  IQQRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIR 120

Query: 121 EEVNSRSTERVFSMTVNLTLWFIEAHHEIGGVVAGENWGMIPACGEAVTKMLKRIEMGED 180
           +E NSRS++RVF MTVNLT+W I A +EIG    G+  GM+PACG AV KML+R++  ++
Sbjct: 121 DEANSRSSDRVFPMTVNLTIWHIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDE 180

Query: 181 -DGVCVICLEEL---KKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICRFQMPI 233
            DG+CVICL+EL   KKKNG + LQMPC H+FH NCIE+WLK SH CPICRF++PI
Sbjct: 181 VDGICVICLDELKNKKKKNGRFTLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 234

[Close]

BLAST of Tan0009133 vs. NCBI nr
Match: XP_011654386.1 (RING finger protein 141 [Cucumis sativus] >KGN54667.1 hypothetical protein Csa_012982 [Cucumis sativus])

HSP 1 Score: 287.3 bits (734), Expect = 1.2e-73
Identity = 147/237 (62.03%), Postives = 175/237 (73.84%), Query Frame = 0


Query: 1   MDYHISRYSNLLVEVATDLPVPRSTKIPKTPSPIELIGLLFTLRHRRSRFDVSENGERQL 60
           M+Y ISRYS   VEV  D+ VP+ TKIPK PSPIELIGLLFTLR R +RF V ENGERQL
Sbjct: 1   MEYQISRYSTTAVEV--DIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQL 60

Query: 61  VWQQEDLKIPLCDHQFRSEIFNRGDDTLFHYLSCSFQSLEQGIDAESVAEALIERWSEIR 120
           V +QE  K+PLCDHQF  EIFN    +LF YL CSF++ E  IDAE VA  LIE+W EIR
Sbjct: 61  VEEQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIR 120

Query: 121 EEVNSRSTERVFSMTVNLTLWFIEAHH---EIGGVVAGENWGMIPACGEAVTKMLKRIEM 180
            +VNSRS ++VFSMTVNLT+  +   +   E G    GE+  ++PAC +A+ KMLKR+EM
Sbjct: 121 NQVNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEM 180

Query: 181 -GEDDGVCVICLEEL-KKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICRFQMPI 233
             ED+ VCVICL+E+ KKKNG  GL+MPC H+FH  CIE W K SHCCPICRF+MPI
Sbjct: 181 REEDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPI 235

[Close]

BLAST of Tan0009133 vs. ExPASy TrEMBL
Match: A0A6J1GK53 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita moschata OX=3662 GN=LOC111455016 PE=4 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 1.2e-84
Identity = 157/236 (66.53%), Postives = 192/236 (81.36%), Query Frame = 0


Query: 1   MDYHISRYSNLLVEVATDLPVPRSTKIPKTPSPIELIGLLFTLRHRRSRFDVSENGERQL 60
           M++ ISRY NL+VEV TDLP P  ++IPK PS IE IGL+FTLR RRS F+VSENGER+L
Sbjct: 1   MEHQISRYENLVVEVLTDLPTP--SRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRL 60

Query: 61  VWQQEDLKIPLCDHQFRSEIFNRGDDTLFHYLSCSFQSLEQGIDAESVAEALIERWSEIR 120
           + Q+E LKIPLCD+QFR+EIFNR DD+LFH LSCSFQ+ +  IDAE VA+ALIERW EIR
Sbjct: 61  IQQRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIR 120

Query: 121 EEVNSRSTERVFSMTVNLTLWFIEAHHEIGGVVAGENWGMIPACGEAVTKMLKRIEMGED 180
           +E NSRS++RVF MTVNLT+W I A +EIG    G+  GM+PACG AV KML+R++  ++
Sbjct: 121 DEANSRSSDRVFPMTVNLTMWHIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDE 180

Query: 181 -DGVCVICLEEL---KKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICRFQMPI 233
            DG+CVICL+EL   KKKNG +GLQMPC H+FH NCIE+WLK SH CPICRF++PI
Sbjct: 181 VDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 234

[Close]

BLAST of Tan0009133 vs. ExPASy TrEMBL
Match: A0A6J1I8N9 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita maxima OX=3661 GN=LOC111471014 PE=4 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 4.6e-84
Identity = 157/236 (66.53%), Postives = 192/236 (81.36%), Query Frame = 0


Query: 1   MDYHISRYSNLLVEVATDLPVPRSTKIPKTPSPIELIGLLFTLRHRRSRFDVSENGERQL 60
           M++ ISRY NL+VEV TDLP P  ++IPK PS IE IGL+FTLRHRRS F+VSE+GER+L
Sbjct: 1   MEHQISRYENLVVEVLTDLPTP--SRIPKHPSRIEHIGLIFTLRHRRSHFEVSESGERRL 60

Query: 61  VWQQEDLKIPLCDHQFRSEIFNRGDDTLFHYLSCSFQSLEQGIDAESVAEALIERWSEIR 120
           + Q+E LKIPLCD+QFR+EIFNR DD+LFH LS SFQ+ +  IDAE VA+ALIERW EIR
Sbjct: 61  IQQRETLKIPLCDNQFRAEIFNRDDDSLFHNLSSSFQNFKPEIDAEPVADALIERWIEIR 120

Query: 121 EEVNSRSTERVFSMTVNLTLWFIEAHHEIGGVVAGENWGMIPACGEAVTKMLKRIEMGED 180
           +E NSRS++RVF MTVNLT+W I A +EIG    G+  GM+PACG AV KML+R+E  ++
Sbjct: 121 DEANSRSSDRVFPMTVNLTMWHIVARYEIGVGSGGDGCGMVPACGGAVGKMLRRLEREDE 180

Query: 181 -DGVCVICLEEL---KKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICRFQMPI 233
            DG+CVICL+EL   KKKNG +GLQMPC H+FH NCIE+WLK SH CPICRF++PI
Sbjct: 181 VDGICVICLDELKKKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 234

[Close]

BLAST of Tan0009133 vs. ExPASy TrEMBL
Match: A0A0A0L236 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G419590 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 5.6e-74
Identity = 147/237 (62.03%), Postives = 175/237 (73.84%), Query Frame = 0


Query: 1   MDYHISRYSNLLVEVATDLPVPRSTKIPKTPSPIELIGLLFTLRHRRSRFDVSENGERQL 60
           M+Y ISRYS   VEV  D+ VP+ TKIPK PSPIELIGLLFTLR R +RF V ENGERQL
Sbjct: 1   MEYQISRYSTTAVEV--DIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQL 60

Query: 61  VWQQEDLKIPLCDHQFRSEIFNRGDDTLFHYLSCSFQSLEQGIDAESVAEALIERWSEIR 120
           V +QE  K+PLCDHQF  EIFN    +LF YL CSF++ E  IDAE VA  LIE+W EIR
Sbjct: 61  VEEQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIR 120

Query: 121 EEVNSRSTERVFSMTVNLTLWFIEAHH---EIGGVVAGENWGMIPACGEAVTKMLKRIEM 180
            +VNSRS ++VFSMTVNLT+  +   +   E G    GE+  ++PAC +A+ KMLKR+EM
Sbjct: 121 NQVNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEM 180

Query: 181 -GEDDGVCVICLEEL-KKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICRFQMPI 233
             ED+ VCVICL+E+ KKKNG  GL+MPC H+FH  CIE W K SHCCPICRF+MPI
Sbjct: 181 REEDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPI 235

[Close]

BLAST of Tan0009133 vs. ExPASy TrEMBL
Match: A0A5A7SNF1 (RING finger protein 141-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00770 PE=4 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 3.1e-72
Identity = 143/241 (59.34%), Postives = 177/241 (73.44%), Query Frame = 0


Query: 1   MDYHISRYSNLLVEVATDLPVPRSTKIPKTPSPIELIGLLFTLRHRRSRFDVSENGERQL 60
           M+Y IS+YS   VE+  DLP+ R  +I K PSPIELIGLLFTLR R +RF V ENGERQL
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSR--RIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQL 60

Query: 61  VWQQEDLKIPLCDHQFRSEIFNRGDDTLFHYLSCSFQSLEQGIDAESVAEALIERWSEIR 120
           V +QE  K+PLCDHQF  EIFN  D++LF YL CSF++ E  IDAE VA ALIE+W  IR
Sbjct: 61  VEEQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIR 120

Query: 121 EEVNSRSTERVFSMTVNLTLWFIEAHH---EIGGVVAGENWGMIPACGEAVTKMLKRIEM 180
           +++NSRS  +VFSMTVNLT+  +   +   E G    GE++  +PAC +A+ KMLKR+E+
Sbjct: 121 DQLNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEV 180

Query: 181 -GEDDGVCVICLEEL-KKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICRFQMPIPQL 237
             ED+ +C+ICL+E+ KKKNG  GL+MPC H+FH  CIE WLK SHCCPICRFQMPI   
Sbjct: 181 RDEDERICIICLDEVRKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMPISSG 239

[Close]

BLAST of Tan0009133 vs. ExPASy TrEMBL
Match: A0A1S4E0Q9 (RING finger protein 141-like OS=Cucumis melo OX=3656 GN=LOC103495859 PE=4 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 3.1e-72
Identity = 143/241 (59.34%), Postives = 177/241 (73.44%), Query Frame = 0


Query: 1   MDYHISRYSNLLVEVATDLPVPRSTKIPKTPSPIELIGLLFTLRHRRSRFDVSENGERQL 60
           M+Y IS+YS   VE+  DLP+ R  +I K PSPIELIGLLFTLR R +RF V ENGERQL
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSR--RIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQL 60

Query: 61  VWQQEDLKIPLCDHQFRSEIFNRGDDTLFHYLSCSFQSLEQGIDAESVAEALIERWSEIR 120
           V +QE  K+PLCDHQF  EIFN  D++LF YL CSF++ E  IDAE VA ALIE+W  IR
Sbjct: 61  VEEQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIR 120

Query: 121 EEVNSRSTERVFSMTVNLTLWFIEAHH---EIGGVVAGENWGMIPACGEAVTKMLKRIEM 180
           +++NSRS  +VFSMTVNLT+  +   +   E G    GE++  +PAC +A+ KMLKR+E+
Sbjct: 121 DQLNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEV 180

Query: 181 -GEDDGVCVICLEEL-KKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICRFQMPIPQL 237
             ED+ +C+ICL+E+ KKKNG  GL+MPC H+FH  CIE WLK SHCCPICRFQMPI   
Sbjct: 181 RDEDERICIICLDEVRKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMPISSG 239

[Close]

BLAST of Tan0009133 vs. TAIR 10
Match: AT1G14200.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 7.1e-13
Identity = 35/85 (41.18%), Postives = 47/85 (55.29%), Query Frame = 0


Query: 155 GENWGMIPACGEAVTKMLKRIEMGED----DGVCVICLEELKKKNGVWGLQMPCSHLFHQ 214
           G   G  PA    V  M  R+ +GED     G C ICL+E  K  G    +MPC H FH 
Sbjct: 77  GGKQGRSPALKSEVENM-PRVVIGEDKEKYGGSCAICLDEWSK--GDVAAEMPCKHKFHS 136

Query: 215 NCIEEWLKKSHCCPICRFQMPIPQL 236
            C+EEWL +   CP+CR++MP+ ++
Sbjct: 137 KCVEEWLGRHATCPMCRYEMPVEEV 158

[Close]

BLAST of Tan0009133 vs. TAIR 10
Match: AT3G15740.1 (RING/U-box superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.2e-12
Identity = 31/70 (44.29%), Postives = 46/70 (65.71%), Query Frame = 0


Query: 162 PACGEAVTKMLKRIEMGEDDGVCVICLEELKKKNGVWGLQMPCSHLFHQNCIEEWLKKSH 221
           PA  EAV   L+ + + E++G CVIC++ ++  + V   +MPCSH+FH+ C EEWL+ S 
Sbjct: 155 PANDEAVEMHLETLVV-ENEGYCVICMDNIRVGSDVEAGRMPCSHVFHRTCGEEWLRNSG 214

Query: 222 CCPICRFQMP 232
            CP+CR   P
Sbjct: 215 ICPVCRALFP 223

[Close]

BLAST of Tan0009133 vs. TAIR 10
Match: AT3G14970.1 (RING/U-box superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.2e-12
Identity = 49/183 (26.78%), Postives = 88/183 (48.09%), Query Frame = 0


Query: 51  DVSENGERQLVWQQEDLKIPLCD--HQFRSEIFNRGDDTLFHYLSCSFQSLEQGIDAESV 110
           +V+ +GE +      DL+  L D  H+F   +   GD      + C    L +      V
Sbjct: 45  EVNSDGEDE---NPLDLETLLVDRTHEFDKGLLFSGDREQIQVIVCHILDLIKAPRYIDV 104

Query: 111 AEALIERWSEIREEVNSRSTERVFSMTVNLTLWFIEAHHEIGGVVAGENWGMIPACGEAV 170
              LI+   +++ + +  S  +   + +N+ +W      +    V      + PA  EAV
Sbjct: 105 VTELIDAILDLK-KCDKISNVKEVDVVINVIVWRFPDDDDDDVDV---RLAVAPASNEAV 164

Query: 171 TKMLKRIEMGEDDGVCVICLEELKKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICRF 230
            + L+ + + E++  CVIC+++++    V   +MPCSH+FH+ C E+WL+ S  CP+CR 
Sbjct: 165 EQHLETVVV-ENESWCVICMDKIRIGLDVAAGRMPCSHVFHRTCGEDWLRCSGICPVCRA 219

Query: 231 QMP 232
             P
Sbjct: 225 MFP 219

[Close]

BLAST of Tan0009133 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 68.9 bits (167), Expect = 6.1e-12
Identity = 30/71 (42.25%), Postives = 42/71 (59.15%), Query Frame = 0


Query: 162 PACGEAVTKMLKRIEMGEDDGVCVICLEELKKKNGVWGLQMPCSHLFHQNCIEEWLKKSH 221
           P   +A    +  +E+   +G CVICLEE K +  V   +MPC H FH  CIE+WL    
Sbjct: 91  PPASKASIDAMPIVEIDGCEGECVICLEEWKSEETV--KEMPCKHRFHGGCIEKWLGFHG 150

Query: 222 CCPICRFQMPI 233
            CP+CR++MP+
Sbjct: 151 SCPVCRYEMPV 159

[Close]

BLAST of Tan0009133 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.5e-10
Identity = 26/64 (40.62%), Postives = 42/64 (65.62%), Query Frame = 0


Query: 168 VTKMLKRIEMGEDDGVCVICLEELKKKNGVWGLQMPCSHLFHQNCIEEWLKKSHCCPICR 227
           VTK + + EM +    C +C++E +  + V   QMPC H+FHQ+C+  WL+  + CP+CR
Sbjct: 204 VTKDMLKSEMNQ----CAVCMDEFEDGSDV--KQMPCKHVFHQDCLLPWLELHNSCPVCR 261

Query: 228 FQMP 232
           F++P
Sbjct: 264 FELP 261

The following BLAST results are available for this feature:

BLAST of Tan0009133 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LQX2	8.5e-11	42.25	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
Q9CY62	7.2e-10	41.67	E3 ubiquitin-protein ligase RNF181 OS=Mus musculus OX=10090 GN=Rnf181 PE=1 SV=1	[more]
Q6AXU4	1.2e-09	40.28	E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 ...	[more]
Q8LPN7	3.6e-09	40.63	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q8GXF8	3.6e-09	49.06	E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 G...	[more]

BLAST of Tan0009133 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571896.1	1.5e-84	66.95	E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022952293.1	2.5e-84	66.53	ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita moschata] >KAG7...	[more]
XP_022972455.1	9.5e-84	66.53	ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita maxima]	[more]
XP_023511497.1	8.0e-83	65.68	ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita pepo subsp. pep...	[more]
XP_011654386.1	1.2e-73	62.03	RING finger protein 141 [Cucumis sativus] >KGN54667.1 hypothetical protein Csa_0...	[more]

BLAST of Tan0009133 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GK53	1.2e-84	66.53	ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita moschata OX=3...	[more]
A0A6J1I8N9	4.6e-84	66.53	ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita maxima OX=366...	[more]
A0A0A0L236	5.6e-74	62.03	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G419590 P...	[more]
A0A5A7SNF1	3.1e-72	59.34	RING finger protein 141-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S4E0Q9	3.1e-72	59.34	RING finger protein 141-like OS=Cucumis melo OX=3656 GN=LOC103495859 PE=4 SV=1	[more]

BLAST of Tan0009133 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14200.1	7.1e-13	41.18	RING/U-box superfamily protein	[more]
AT3G15740.1	1.2e-12	44.29	RING/U-box superfamily protein	[more]
AT3G14970.1	1.2e-12	26.78	RING/U-box superfamily protein	[more]
AT1G26800.1	6.1e-12	42.25	RING/U-box superfamily protein	[more]
AT3G19950.1	2.5e-10	40.63	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 184..226 e-value: 1.8E-6 score: 37.5
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 184..227 e-value: 2.3E-12 score: 47.0
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 184..227 score: 12.917115
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 161..233 e-value: 4.4E-17 score: 63.5
None	No IPR available	PANTHER	PTHR15710:SF63	RING/U-BOX SUPERFAMILY PROTEIN	coord: 90..231
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 90..231
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 177..232

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0009133.1	Tan0009133.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity