Tan0009131 (gene) Snake gourd v1

BLAST of Tan0009131 vs. ExPASy Swiss-Prot
Match: Q84UB9 (Delta(12)-oleate desaturase OS=Trichosanthes kirilowii OX=3677 GN=FAD2 PE=2 SV=1)

HSP 1 Score: 764.2 bits (1972), Expect = 6.8e-220
Identity = 355/369 (96.21%), Postives = 363/369 (98.37%), Query Frame = 0

Query: 7   MEKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYLP 66
           MEKGV ERVPHAKPPFTLSQIKK VPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAY P
Sbjct: 1   MEKGVQERVPHAKPPFTLSQIKKVVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYFP 60

Query: 67  LLPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYF 126
           LLPHPFPYIAWPLYWIFQGC+LTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYF
Sbjct: 61  LLPHPFPYIAWPLYWIFQGCSLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYF 120

Query: 127 SWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLISLIGTLTLGWPLYLA 186
           SWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWY+KYFNNPPGRLISLIGTLTLGWPLYLA
Sbjct: 121 SWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYLKYFNNPPGRLISLIGTLTLGWPLYLA 180

Query: 187 FNVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAFWVMRIY 246
           FN+SGRPYDRFACHYDPY PIFTKREWIQVYISDAGILGMAF+LYRIGV KG+FWVMRIY
Sbjct: 181 FNMSGRPYDRFACHYDPYSPIFTKREWIQVYISDAGILGMAFMLYRIGVEKGSFWVMRIY 240

Query: 247 GIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH 306
           GIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH
Sbjct: 241 GIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH 300

Query: 307 VVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAKECVYVEPDENANSDK 366
           VVHHLFSTMPHYHAKEATEAVKPVLGEYYRLD+TPVLKAIWREAKECVYVEPDE+A+SDK
Sbjct: 301 VVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVEPDEDADSDK 360

Query: 367 GVFWYRKKL 376
           GV WYRKKL
Sbjct: 361 GVVWYRKKL 369

BLAST of Tan0009131 vs. ExPASy Swiss-Prot
Match: Q84VT2 (Delta(12)-acyl-lipid-desaturase OS=Punica granatum OX=22663 GN=FAD12 PE=2 SV=2)

HSP 1 Score: 613.6 bits (1581), Expect = 1.5e-174
Identity = 273/374 (72.99%), Postives = 318/374 (85.03%), Query Frame = 0

Query: 2   GVGSRMEKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLA 61
           G G    +   +RVP AKPPFTLS+IKKA+PPHCF RSLL+SFSY+L DL  V++F+Y+A
Sbjct: 15  GEGDEKSQKPVQRVPSAKPPFTLSEIKKAIPPHCFKRSLLKSFSYVLYDLTLVAIFYYVA 74

Query: 62  IAYLPLLPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSAL 121
             Y+  LP P  Y AWP+YW  QGC LTGVWVIAHECGHHAFSDYQ +DD VGL  HSAL
Sbjct: 75  TTYIDALPGPLRYAAWPVYWALQGCVLTGVWVIAHECGHHAFSDYQWVDDCVGLVLHSAL 134

Query: 122 LVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLISLIGTLTLGW 181
           LVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKS++PW+ KY NNPPGR+++LI TLTLGW
Sbjct: 135 LVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSKMPWFSKYLNNPPGRVMTLIVTLTLGW 194

Query: 182 PLYLAFNVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAFW 241
           PLYLA NVSG PYDRFACH+DPYGPI+T RE +Q+YISD GI+   + LY+I  A+G  W
Sbjct: 195 PLYLALNVSGWPYDRFACHFDPYGPIYTDRERLQIYISDVGIMAATYTLYKIAAARGLAW 254

Query: 242 VMRIYGIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHN 301
           ++ +YG+PL+IVN FLV ITYLQHTHPALPHY+SSEWDWLRGALAT DRDYG+LN+VFHN
Sbjct: 255 LVCVYGVPLLIVNAFLVTITYLQHTHPALPHYDSSEWDWLRGALATADRDYGILNKVFHN 314

Query: 302 ITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAKECVYVEPDEN 361
           ITDTHV HHLFSTMPHYHA EAT+A+KP+LG+YY+ D TPV KA+WREA+EC+YVEPD+ 
Sbjct: 315 ITDTHVAHHLFSTMPHYHAMEATKAIKPILGDYYQFDGTPVYKAMWREARECLYVEPDDG 374

Query: 362 ANSDKGVFWYRKKL 376
           ANS KGVFWY+K L
Sbjct: 375 ANS-KGVFWYKKNL 387

BLAST of Tan0009131 vs. ExPASy Swiss-Prot
Match: Q8GZC3 (Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 1.2e-173
Identity = 276/383 (72.06%), Postives = 316/383 (82.51%), Query Frame = 0

Query: 1   MGVGSRM---------EKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDL 60
           MG G RM         E  V +RVPH+KPPFTL Q+KKA+PPHCF RS+LRSFSY++ DL
Sbjct: 1   MGAGGRMSVPPPPKKLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  AFVSLFFYLAIAYLPLLPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDD 120
               +F+Y+A  Y  LLP P  Y+AWP+YW  QGC LTGVWVIAHECGHHAFSDYQL+DD
Sbjct: 61  TVAFIFYYIATNYFHLLPQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDD 120

Query: 121 IVGLFFHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLI 180
           IVGL  HS LLVPYFSWK+SHRRHHSNT SLERDEVFVPK KS I W+ KY NNPPGRL 
Sbjct: 121 IVGLVLHSCLLVPYFSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLF 180

Query: 181 SLIGTLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLY 240
           +L  TLTLGWPLYLAFNVSGRPYDRFACHYDPYGPI+T RE  ++YISDAG+L + F LY
Sbjct: 181 TLTITLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLY 240

Query: 241 RIGVAKGAFWVMRIYGIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRD 300
           R+  AKG  WV+ +YG+PL+IVN FLV+ITYLQHTHP++PHY+SSEWDWLRGALATVDRD
Sbjct: 241 RLAAAKGLAWVICVYGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGVLNRVFHNITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAK 360
           YG+LN+VFHNITDTHV HHLFSTMPHYHA EAT+A+KP+LGEYY+ D TP  KA+WREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAK 360

Query: 361 ECVYVEPDENANSDKGVFWYRKK 375
           EC+YVE D+   S KGV+WY KK
Sbjct: 361 ECIYVEADDGDES-KGVYWYNKK 382

BLAST of Tan0009131 vs. ExPASy Swiss-Prot
Match: P48631 (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max OX=3847 GN=FAD2-2 PE=2 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 1.2e-168
Identity = 267/363 (73.55%), Postives = 309/363 (85.12%), Query Frame = 0

Query: 13  ERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYLPLLPHPF 72
           +RVP  KP F+LSQIKKA+PPHCF RS+LRSFSY++ DL      +Y+A  Y  LLP P 
Sbjct: 22  KRVPFEKPQFSLSQIKKAIPPHCFQRSVLRSFSYVVYDLTIAFCLYYVATHYFHLLPGPL 81

Query: 73  PYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYFSWKYSH 132
            +    +YW  QGC LTGVWVIAHECGHHAFSDYQL+DDIVGL  HSALLVPYFSWKYSH
Sbjct: 82  SFRGMAIYWAVQGCILTGVWVIAHECGHHAFSDYQLLDDIVGLILHSALLVPYFSWKYSH 141

Query: 133 RRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLISLIGTLTLGWPLYLAFNVSGR 192
           RRHHSNTGSLERDEVFVPK KS I WY KY NNPPGR+++L  TLTLGWPLYLA NVSGR
Sbjct: 142 RRHHSNTGSLERDEVFVPKQKSCIKWYSKYLNNPPGRVLTLAVTLTLGWPLYLALNVSGR 201

Query: 193 PYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAFWVMRIYGIPLVI 252
           PYDRFACHYDPYGPI++ RE +Q+YISDAG+L + + L+R+ +AKG  WV+ +YG+PL++
Sbjct: 202 PYDRFACHYDPYGPIYSDRERLQIYISDAGVLAVVYGLFRLAMAKGLAWVVCVYGVPLLV 261

Query: 253 VNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTHVVHHLF 312
           VNGFLVLIT+LQHTHPALPHY SSEWDWLRGALATVDRDYG+LN+VFHNITDTHV HHLF
Sbjct: 262 VNGFLVLITFLQHTHPALPHYTSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLF 321

Query: 313 STMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAKECVYVEPDENANSDKGVFWYR 372
           STMPHYHA EAT+A+KP+LGEYYR D+TP +KA+WREA+EC+YVEPD++  S KGVFWY 
Sbjct: 322 STMPHYHAMEATKAIKPILGEYYRFDETPFVKAMWREARECIYVEPDQSTES-KGVFWYN 381

Query: 373 KKL 376
            KL
Sbjct: 382 NKL 383

BLAST of Tan0009131 vs. ExPASy Swiss-Prot
Match: Q41131 (Oleate hydroxylase FAH12 OS=Ricinus communis OX=3988 GN=FAH12 PE=1 SV=2)

HSP 1 Score: 582.0 bits (1499), Expect = 4.8e-165
Identity = 258/362 (71.27%), Postives = 302/362 (83.43%), Query Frame = 0

Query: 13  ERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYLPLLPHPF 72
           +R PH KPPFTL  +K+A+PPHCF RS +RSFSY+  D+    LF+ +A  + P +  P 
Sbjct: 26  KRAPHTKPPFTLGDLKRAIPPHCFERSFVRSFSYVAYDVCLSFLFYSIATNFFPYISSPL 85

Query: 73  PYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYFSWKYSH 132
            Y+AW +YW+FQGC LTG+WVI HECGHHAFS+YQL DDIVGL  HSALLVPYFSWKYSH
Sbjct: 86  SYVAWLVYWLFQGCILTGLWVIGHECGHHAFSEYQLADDIVGLIVHSALLVPYFSWKYSH 145

Query: 133 RRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLISLIGTLTLGWPLYLAFNVSGR 192
           RRHHSN GSLERDEVFVPK KS+I WY KY NNPPGR+++L  TL LGWPLYLAFNVSGR
Sbjct: 146 RRHHSNIGSLERDEVFVPKSKSKISWYSKYLNNPPGRVLTLAATLLLGWPLYLAFNVSGR 205

Query: 193 PYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAFWVMRIYGIPLVI 252
           PYDRFACHYDPYGPIF++RE +Q+YI+D GI    FVLY+  +AKG  WVMRIYG+PL+I
Sbjct: 206 PYDRFACHYDPYGPIFSERERLQIYIADLGIFATTFVLYQATMAKGLAWVMRIYGVPLLI 265

Query: 253 VNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTHVVHHLF 312
           VN FLV+ITYLQHTHPA+P Y SSEWDWLRGA+ TVDRDYGVLN+VFHNI DTHV HHLF
Sbjct: 266 VNCFLVMITYLQHTHPAIPRYGSSEWDWLRGAMVTVDRDYGVLNKVFHNIADTHVAHHLF 325

Query: 313 STMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAKECVYVEPDENANSDKGVFWYR 372
           +T+PHYHA EAT+A+KP++GEYYR D TP  KA+WREAKEC++VEPDE A + +GVFWYR
Sbjct: 326 ATVPHYHAMEATKAIKPIMGEYYRYDGTPFYKALWREAKECLFVEPDEGAPT-QGVFWYR 385

Query: 373 KK 375
            K
Sbjct: 386 NK 386

BLAST of Tan0009131 vs. NCBI nr
Match: Q84UB9.1 (RecName: Full=Delta(12)-oleate desaturase; Short=PgFAD2 [Trichosanthes kirilowii] >AAO37752.1 delta-12 oleate desaturase [Trichosanthes kirilowii])

HSP 1 Score: 764.2 bits (1972), Expect = 5.2e-217
Identity = 355/369 (96.21%), Postives = 363/369 (98.37%), Query Frame = 0

Query: 7   MEKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYLP 66
           MEKGV ERVPHAKPPFTLSQIKK VPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAY P
Sbjct: 1   MEKGVQERVPHAKPPFTLSQIKKVVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYFP 60

Query: 67  LLPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYF 126
           LLPHPFPYIAWPLYWIFQGC+LTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYF
Sbjct: 61  LLPHPFPYIAWPLYWIFQGCSLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYF 120

Query: 127 SWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLISLIGTLTLGWPLYLA 186
           SWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWY+KYFNNPPGRLISLIGTLTLGWPLYLA
Sbjct: 121 SWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYLKYFNNPPGRLISLIGTLTLGWPLYLA 180

Query: 187 FNVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAFWVMRIY 246
           FN+SGRPYDRFACHYDPY PIFTKREWIQVYISDAGILGMAF+LYRIGV KG+FWVMRIY
Sbjct: 181 FNMSGRPYDRFACHYDPYSPIFTKREWIQVYISDAGILGMAFMLYRIGVEKGSFWVMRIY 240

Query: 247 GIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH 306
           GIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH
Sbjct: 241 GIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH 300

Query: 307 VVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAKECVYVEPDENANSDK 366
           VVHHLFSTMPHYHAKEATEAVKPVLGEYYRLD+TPVLKAIWREAKECVYVEPDE+A+SDK
Sbjct: 301 VVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVEPDEDADSDK 360

Query: 367 GVFWYRKKL 376
           GV WYRKKL
Sbjct: 361 GVVWYRKKL 369

BLAST of Tan0009131 vs. NCBI nr
Match: KAG6570369.1 (Delta(12)-oleate desaturase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 756.9 bits (1953), Expect = 8.2e-215
Identity = 348/375 (92.80%), Postives = 364/375 (97.07%), Query Frame = 0

Query: 1   MGVGSRMEKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYL 60
           MGVGS+MEK VHERVPHAKPPFTLSQIKKA+PPHCFHRSLLRSFS L+SDLAFVSLFFYL
Sbjct: 1   MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSCLISDLAFVSLFFYL 60

Query: 61  AIAYLPLLPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA 120
           AIAYLPL+PHP  YIAWPLYW+FQGC LTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA
Sbjct: 61  AIAYLPLIPHPASYIAWPLYWVFQGCILTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA 120

Query: 121 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLISLIGTLTLG 180
           LLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPW++KYFNNPPGRLISL+GTLTLG
Sbjct: 121 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWFLKYFNNPPGRLISLLGTLTLG 180

Query: 181 WPLYLAFNVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAF 240
           WPLYLAFNVSGRPYDRFACHYDP+GPIFTKREW+QVYISD GILGM FV+YRIGVAKGA 
Sbjct: 181 WPLYLAFNVSGRPYDRFACHYDPHGPIFTKREWVQVYISDTGILGMCFVVYRIGVAKGAL 240

Query: 241 WVMRIYGIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFH 300
           WVMRIYGIPL+IVNGFLVLITYLQHTHPALPHY+S+EWDWLRGALATVDRDYGVLNRVFH
Sbjct: 241 WVMRIYGIPLLIVNGFLVLITYLQHTHPALPHYDSAEWDWLRGALATVDRDYGVLNRVFH 300

Query: 301 NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAKECVYVEPDE 360
           NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLD+TPVLKAIWREAKECVYV PDE
Sbjct: 301 NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVAPDE 360

Query: 361 NANSDKGVFWYRKKL 376
           +ANSDKGV WYR KL
Sbjct: 361 DANSDKGVVWYRNKL 375

BLAST of Tan0009131 vs. NCBI nr
Match: XP_022943671.1 (delta(12)-oleate desaturase [Cucurbita moschata])

HSP 1 Score: 753.4 bits (1944), Expect = 9.1e-214
Identity = 346/375 (92.27%), Postives = 363/375 (96.80%), Query Frame = 0

Query: 1   MGVGSRMEKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYL 60
           MGVGS+MEK VHERVPHAKPPFTLSQIKKA+PPHCFHRSLLRSFS L+SDLAFVSLFFYL
Sbjct: 1   MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSCLISDLAFVSLFFYL 60

Query: 61  AIAYLPLLPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA 120
           AIAYLPL+PHP  YIAWPLYW+FQGC LTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA
Sbjct: 61  AIAYLPLIPHPASYIAWPLYWVFQGCILTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA 120

Query: 121 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLISLIGTLTLG 180
           LLVPYFSWKYSHRRHHSNT SLERDEVFVPKPKSQIPW++KYFNNPPGRLISL+GTLTLG
Sbjct: 121 LLVPYFSWKYSHRRHHSNTVSLERDEVFVPKPKSQIPWFLKYFNNPPGRLISLLGTLTLG 180

Query: 181 WPLYLAFNVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAF 240
           WPLYLAFNVSGRPYDRFACHYDP+GPIFTKREW+Q+YISD GILGM FVLYRIGVAKGA 
Sbjct: 181 WPLYLAFNVSGRPYDRFACHYDPHGPIFTKREWVQIYISDTGILGMCFVLYRIGVAKGAL 240

Query: 241 WVMRIYGIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFH 300
           WVMRIYGIPL+IVNGFLVLITYLQHTHPALPHY+++EWDWLRGALATVDRDYGVLNRVFH
Sbjct: 241 WVMRIYGIPLLIVNGFLVLITYLQHTHPALPHYDAAEWDWLRGALATVDRDYGVLNRVFH 300

Query: 301 NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAKECVYVEPDE 360
           NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLD+TPVLKAIWREAKECVYV PDE
Sbjct: 301 NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVAPDE 360

Query: 361 NANSDKGVFWYRKKL 376
           +ANSDKGV WYR KL
Sbjct: 361 DANSDKGVIWYRNKL 375

BLAST of Tan0009131 vs. NCBI nr
Match: XP_023512996.1 (delta(12)-oleate desaturase [Cucurbita pepo subsp. pepo] >XP_023522472.1 delta(12)-oleate desaturase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 749.2 bits (1933), Expect = 1.7e-212
Identity = 344/375 (91.73%), Postives = 360/375 (96.00%), Query Frame = 0

Query: 1   MGVGSRMEKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYL 60
           MGVGS+MEK VHERVPHAKPPFTLSQIKKA+PPHCFHRSLLRSFS L+SDLAFVSLFFYL
Sbjct: 1   MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSCLISDLAFVSLFFYL 60

Query: 61  AIAYLPLLPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA 120
           AIAYLPL+PHP  YIAWP YW+FQGC LTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA
Sbjct: 61  AIAYLPLIPHPASYIAWPFYWVFQGCILTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA 120

Query: 121 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLISLIGTLTLG 180
           LLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPW++KYFNNPPGRLISL+GTLTLG
Sbjct: 121 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWFLKYFNNPPGRLISLLGTLTLG 180

Query: 181 WPLYLAFNVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAF 240
           WPLYLAFNVSGRPYDRFACHYDP+GPIFTKREW+Q+YISD GILGM  VLYRI VAKGA 
Sbjct: 181 WPLYLAFNVSGRPYDRFACHYDPHGPIFTKREWVQIYISDTGILGMCLVLYRISVAKGAL 240

Query: 241 WVMRIYGIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFH 300
           WVMRIYGIPL+IVNGFLVLITYLQHTHPALPHY+S+EWDWLRGALATVDRDYGVLNRVFH
Sbjct: 241 WVMRIYGIPLLIVNGFLVLITYLQHTHPALPHYDSAEWDWLRGALATVDRDYGVLNRVFH 300

Query: 301 NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAKECVYVEPDE 360
           NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLD+TPVLKAIWREAKECVYV PDE
Sbjct: 301 NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVAPDE 360

Query: 361 NANSDKGVFWYRKKL 376
           + NSDKGV WYR KL
Sbjct: 361 DDNSDKGVVWYRNKL 375

BLAST of Tan0009131 vs. NCBI nr
Match: XP_022995104.1 (delta(12)-oleate desaturase [Cucurbita maxima])

HSP 1 Score: 726.5 bits (1874), Expect = 1.2e-205
Identity = 332/366 (90.71%), Postives = 349/366 (95.36%), Query Frame = 0

Query: 8   EKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYLPL 67
           EKG HERVPHAKPPFTL+QIKKA+PPHCFHRSLLRSF+ LLSDLA VSLFFYLAIAY PL
Sbjct: 3   EKGTHERVPHAKPPFTLTQIKKAIPPHCFHRSLLRSFTCLLSDLASVSLFFYLAIAYFPL 62

Query: 68  LPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYFS 127
           +P+PFPYIAWPLYW+FQGCTLTG+WVIAHECGHHAFSDYQLIDD VG F HSALLVPYFS
Sbjct: 63  IPYPFPYIAWPLYWVFQGCTLTGIWVIAHECGHHAFSDYQLIDDTVGFFLHSALLVPYFS 122

Query: 128 WKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLISLIGTLTLGWPLYLAF 187
           WKYSHRRHHSNTGSLERDEVFVPKPKSQ+PWY KYFNNPPGRLISLI TLTLGWPLYLAF
Sbjct: 123 WKYSHRRHHSNTGSLERDEVFVPKPKSQVPWYSKYFNNPPGRLISLIATLTLGWPLYLAF 182

Query: 188 NVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAFWVMRIYG 247
           NVSGRPYDR ACHYDPY PIFTKREWIQVYISDAGILGMAFVLYRI +AKGAFWVMRIYG
Sbjct: 183 NVSGRPYDRLACHYDPYAPIFTKREWIQVYISDAGILGMAFVLYRIAMAKGAFWVMRIYG 242

Query: 248 IPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTHV 307
           IPL+IVNGFLVLIT+LQHTHPALPHY+SSEW+WLRGALATVDRDYGVLNRVFHNITDTHV
Sbjct: 243 IPLLIVNGFLVLITFLQHTHPALPHYDSSEWNWLRGALATVDRDYGVLNRVFHNITDTHV 302

Query: 308 VHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAKECVYVEPDENANSDKG 367
            HHLFSTMPHYHAKEATEA+KPVLGEYYR D+TPVLKAIWRE KECVYVE DE+ANS+KG
Sbjct: 303 AHHLFSTMPHYHAKEATEAIKPVLGEYYRFDRTPVLKAIWRETKECVYVEADEDANSEKG 362

Query: 368 VFWYRK 374
           V WYRK
Sbjct: 363 VVWYRK 368

BLAST of Tan0009131 vs. ExPASy TrEMBL
Match: A0A6J1FSC4 (delta(12)-oleate desaturase OS=Cucurbita moschata OX=3662 GN=LOC111448353 PE=3 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 4.4e-214
Identity = 346/375 (92.27%), Postives = 363/375 (96.80%), Query Frame = 0

Query: 1   MGVGSRMEKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYL 60
           MGVGS+MEK VHERVPHAKPPFTLSQIKKA+PPHCFHRSLLRSFS L+SDLAFVSLFFYL
Sbjct: 1   MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSCLISDLAFVSLFFYL 60

Query: 61  AIAYLPLLPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA 120
           AIAYLPL+PHP  YIAWPLYW+FQGC LTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA
Sbjct: 61  AIAYLPLIPHPASYIAWPLYWVFQGCILTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA 120

Query: 121 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLISLIGTLTLG 180
           LLVPYFSWKYSHRRHHSNT SLERDEVFVPKPKSQIPW++KYFNNPPGRLISL+GTLTLG
Sbjct: 121 LLVPYFSWKYSHRRHHSNTVSLERDEVFVPKPKSQIPWFLKYFNNPPGRLISLLGTLTLG 180

Query: 181 WPLYLAFNVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAF 240
           WPLYLAFNVSGRPYDRFACHYDP+GPIFTKREW+Q+YISD GILGM FVLYRIGVAKGA 
Sbjct: 181 WPLYLAFNVSGRPYDRFACHYDPHGPIFTKREWVQIYISDTGILGMCFVLYRIGVAKGAL 240

Query: 241 WVMRIYGIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFH 300
           WVMRIYGIPL+IVNGFLVLITYLQHTHPALPHY+++EWDWLRGALATVDRDYGVLNRVFH
Sbjct: 241 WVMRIYGIPLLIVNGFLVLITYLQHTHPALPHYDAAEWDWLRGALATVDRDYGVLNRVFH 300

Query: 301 NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAKECVYVEPDE 360
           NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLD+TPVLKAIWREAKECVYV PDE
Sbjct: 301 NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVAPDE 360

Query: 361 NANSDKGVFWYRKKL 376
           +ANSDKGV WYR KL
Sbjct: 361 DANSDKGVIWYRNKL 375

BLAST of Tan0009131 vs. ExPASy TrEMBL
Match: A0A6J1K4R6 (delta(12)-oleate desaturase OS=Cucurbita maxima OX=3661 GN=LOC111490747 PE=4 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 5.8e-206
Identity = 332/366 (90.71%), Postives = 349/366 (95.36%), Query Frame = 0

Query: 8   EKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYLPL 67
           EKG HERVPHAKPPFTL+QIKKA+PPHCFHRSLLRSF+ LLSDLA VSLFFYLAIAY PL
Sbjct: 3   EKGTHERVPHAKPPFTLTQIKKAIPPHCFHRSLLRSFTCLLSDLASVSLFFYLAIAYFPL 62

Query: 68  LPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYFS 127
           +P+PFPYIAWPLYW+FQGCTLTG+WVIAHECGHHAFSDYQLIDD VG F HSALLVPYFS
Sbjct: 63  IPYPFPYIAWPLYWVFQGCTLTGIWVIAHECGHHAFSDYQLIDDTVGFFLHSALLVPYFS 122

Query: 128 WKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLISLIGTLTLGWPLYLAF 187
           WKYSHRRHHSNTGSLERDEVFVPKPKSQ+PWY KYFNNPPGRLISLI TLTLGWPLYLAF
Sbjct: 123 WKYSHRRHHSNTGSLERDEVFVPKPKSQVPWYSKYFNNPPGRLISLIATLTLGWPLYLAF 182

Query: 188 NVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAFWVMRIYG 247
           NVSGRPYDR ACHYDPY PIFTKREWIQVYISDAGILGMAFVLYRI +AKGAFWVMRIYG
Sbjct: 183 NVSGRPYDRLACHYDPYAPIFTKREWIQVYISDAGILGMAFVLYRIAMAKGAFWVMRIYG 242

Query: 248 IPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTHV 307
           IPL+IVNGFLVLIT+LQHTHPALPHY+SSEW+WLRGALATVDRDYGVLNRVFHNITDTHV
Sbjct: 243 IPLLIVNGFLVLITFLQHTHPALPHYDSSEWNWLRGALATVDRDYGVLNRVFHNITDTHV 302

Query: 308 VHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAKECVYVEPDENANSDKG 367
            HHLFSTMPHYHAKEATEA+KPVLGEYYR D+TPVLKAIWRE KECVYVE DE+ANS+KG
Sbjct: 303 AHHLFSTMPHYHAKEATEAIKPVLGEYYRFDRTPVLKAIWRETKECVYVEADEDANSEKG 362

Query: 368 VFWYRK 374
           V WYRK
Sbjct: 363 VVWYRK 368

BLAST of Tan0009131 vs. ExPASy TrEMBL
Match: A0A6J1H0J3 (delta(12)-oleate desaturase-like OS=Cucurbita moschata OX=3662 GN=LOC111459302 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 2.9e-205
Identity = 334/368 (90.76%), Postives = 352/368 (95.65%), Query Frame = 0

Query: 8   EKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYLPL 67
           EKGVHERVPHAKPPFTL+QIKKA+PPHCFHRSLLRSF+ LLSDLA VSLF YLAIA+  L
Sbjct: 3   EKGVHERVPHAKPPFTLTQIKKAIPPHCFHRSLLRSFTCLLSDLASVSLFSYLAIAHFRL 62

Query: 68  LPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYFS 127
           +P+PFPYIAWPLYW+FQGCTLTG+WVIAHECGHHAFSDYQLIDDIVG F HSALLVPYFS
Sbjct: 63  IPYPFPYIAWPLYWVFQGCTLTGIWVIAHECGHHAFSDYQLIDDIVGFFLHSALLVPYFS 122

Query: 128 WKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLISLIGTLTLGWPLYLAF 187
           WKYSHRRHHSNTGSLERDEVFVPKPKSQIPWY KYFNNPPGRLISLI TLTLGWPLYLAF
Sbjct: 123 WKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYSKYFNNPPGRLISLIATLTLGWPLYLAF 182

Query: 188 NVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAFWVMRIYG 247
           NVSGRPYDR+ACHYDPY PIFTKREWIQVYISDAGILGMAFVLYRI +AKGAFWVMRIYG
Sbjct: 183 NVSGRPYDRWACHYDPYAPIFTKREWIQVYISDAGILGMAFVLYRIAMAKGAFWVMRIYG 242

Query: 248 IPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTHV 307
           IPL+IVNGFLVLIT+LQHTHPALPHY+SSEW+WLRGALATVDRDYGVLNRVFHNITDTHV
Sbjct: 243 IPLLIVNGFLVLITFLQHTHPALPHYDSSEWNWLRGALATVDRDYGVLNRVFHNITDTHV 302

Query: 308 VHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAKECVYVEPDENANSDKG 367
            HHLFSTMPHYHAKEATEA+K VLGEYYRLD+TPVLKAIWREAKECVYVE DE+ANS+KG
Sbjct: 303 AHHLFSTMPHYHAKEATEAIKAVLGEYYRLDRTPVLKAIWREAKECVYVEADEDANSEKG 362

Query: 368 VFWYRKKL 376
           V WYRK L
Sbjct: 363 VVWYRKNL 370

BLAST of Tan0009131 vs. ExPASy TrEMBL
Match: W9QMT8 (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Morus notabilis OX=981085 GN=L484_018419 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 4.6e-179
Identity = 290/385 (75.32%), Postives = 328/385 (85.19%), Query Frame = 0

Query: 1   MGVGSRM----------EKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSD 60
           MG G RM           K   ERVPH  PPFTLSQIKKA+PPHCF RSL+RSFSY+  D
Sbjct: 1   MGAGGRMPVLSSNKLEGHKSQVERVPHTTPPFTLSQIKKAIPPHCFKRSLVRSFSYVGHD 60

Query: 61  LAFVSLFFYLAIAYLPLLPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLID 120
           L F +LF+Y+A +Y  LLPHP PY+AWPLYWI QGC  TG+WVIAHECGHHAFSD+Q +D
Sbjct: 61  LFFAALFYYIATSYFHLLPHPLPYLAWPLYWIVQGCVCTGIWVIAHECGHHAFSDHQWVD 120

Query: 121 DIVGLFFHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRL 180
           D VGL  HSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKS+I WY KY NNPPGRL
Sbjct: 121 DTVGLILHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSKISWYSKYLNNPPGRL 180

Query: 181 ISLIGTLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVL 240
           +SL+ TLTLGWPLYLAFNVSGRPYDRFACHYDPYGPI++ RE +Q+++SDAGI    FVL
Sbjct: 181 LSLLVTLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFVSDAGIFVTTFVL 240

Query: 241 YRIGVAKGAFWVMRIYGIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDR 300
           Y++  AKG  W++ +YG+PL+IVNGFLVLITYLQHTHPALPHY+SSEWDWLRGALATVDR
Sbjct: 241 YQLAAAKGLAWIVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDR 300

Query: 301 DYGVLNRVFHNITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREA 360
           DYGVLNRVFHNI DTHV HHLFSTMPHY+A EAT+AVKP+LGEYYR D+TP+ KA+WREA
Sbjct: 301 DYGVLNRVFHNINDTHVAHHLFSTMPHYNAMEATKAVKPLLGEYYRFDRTPIFKAMWREA 360

Query: 361 KECVYVEPDENANSDKGVFWYRKKL 376
           KEC+YVEPD++A  +KGVFWYR KL
Sbjct: 361 KECLYVEPDDDA-PNKGVFWYRNKL 384

BLAST of Tan0009131 vs. ExPASy TrEMBL
Match: A0A2P5EEE4 (Fatty acid desaturase OS=Trema orientale OX=63057 GN=TorRG33x02_201950 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 7.8e-179
Identity = 291/384 (75.78%), Postives = 330/384 (85.94%), Query Frame = 0

Query: 1   MGVGSRM----------EKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSD 60
           MG G RM          +K   ERVPH  PPFTL QIKKA+PPHCF RSLLRSFSY++ D
Sbjct: 1   MGAGGRMPVPNTNLEGGKKSPVERVPHTTPPFTLGQIKKAIPPHCFKRSLLRSFSYVVHD 60

Query: 61  LAFVSLFFYLAIAYLPLLPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLID 120
           L F SLF+Y+A +Y  LLPHP  Y AWPLYWIFQGC  TGVWVIAHECGHHAFSD+Q +D
Sbjct: 61  LFFASLFYYVATSYFHLLPHPLSYFAWPLYWIFQGCVSTGVWVIAHECGHHAFSDHQWVD 120

Query: 121 DIVGLFFHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRL 180
           DIVGL  HSALLVPYFSWKYSHRRHHSNTGS+ERDEVFVPKPKSQ+ WY KY NNPPGR+
Sbjct: 121 DIVGLVLHSALLVPYFSWKYSHRRHHSNTGSIERDEVFVPKPKSQMAWYSKYLNNPPGRV 180

Query: 181 ISLIGTLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVL 240
           +SL+ TLTLGWPLYLAFNVSGRPYDRFACHYDPYGPI++ RE +Q++ISDAGI  M FVL
Sbjct: 181 LSLLVTLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGIFLMTFVL 240

Query: 241 YRIGVAKGAFWVMRIYGIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDR 300
           Y++  AKG  WV+ +YG+PL+IVNGFLVLITYLQHTHPALPHY+SSEWDWLRGAL+TVDR
Sbjct: 241 YQLAAAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALSTVDR 300

Query: 301 DYGVLNRVFHNITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREA 360
           DYGVLN+VFHNITDTHVVHHLFSTMPHY+A EAT+AVKP+LG+YYR D TP+++A+WREA
Sbjct: 301 DYGVLNKVFHNITDTHVVHHLFSTMPHYNAMEATKAVKPLLGDYYRFDGTPIVEAMWREA 360

Query: 361 KECVYVEPDENANSDKGVFWYRKK 375
           KEC+YVEPD+ A  +KGVFWYR K
Sbjct: 361 KECLYVEPDDEA-PNKGVFWYRNK 383

BLAST of Tan0009131 vs. TAIR 10
Match: AT3G12120.1 (fatty acid desaturase 2 )

HSP 1 Score: 562.0 bits (1447), Expect = 3.6e-160
Identity = 259/384 (67.45%), Postives = 301/384 (78.39%), Query Frame = 0

Query: 1   MGVGSRM---------EKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDL 60
           MG G RM         E    +RVP  KPPF++  +KKA+PPHCF RS+ RSFSYL+SD+
Sbjct: 1   MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60

Query: 61  AFVSLFFYLAIAYLPLLPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDD 120
              S F+Y+A  Y  LLP P  Y+AWPLYW  QGC LTG+WVIAHECGHHAFSDYQ +DD
Sbjct: 61  IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120

Query: 121 IVGLFFHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLI 180
            VGL FHS LLVPYFSWKYSHRRHHSNTGSLERDEVFVPK KS I WY KY NNP GR++
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 180

Query: 181 SLIGTLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLY 240
            L     LGWPLYLAFNVSGRPYD FACH+ P  PI+  RE +Q+Y+SDAGIL + F LY
Sbjct: 181 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 240

Query: 241 RIGVAKGAFWVMRIYGIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRD 300
           R   A+G   ++ +YG+PL+IVN FLVLITYLQHTHP+LPHY+SSEWDWLRGALATVDRD
Sbjct: 241 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGVLNRVFHNITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAK 360
           YG+LN+VFHNITDTHV HHLFSTMPHY+A EAT+A+KP+LG+YY+ D TP   A++REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 360

Query: 361 ECVYVEPDENANSDKGVFWYRKKL 376
           EC+YVEPD   +  KGV+WY  KL
Sbjct: 361 ECIYVEPDREGDK-KGVYWYNNKL 383

BLAST of Tan0009131 vs. TAIR 10
Match: AT3G12120.2 (fatty acid desaturase 2 )

HSP 1 Score: 562.0 bits (1447), Expect = 3.6e-160
Identity = 259/384 (67.45%), Postives = 301/384 (78.39%), Query Frame = 0

Query: 1   MGVGSRM---------EKGVHERVPHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDL 60
           MG G RM         E    +RVP  KPPF++  +KKA+PPHCF RS+ RSFSYL+SD+
Sbjct: 1   MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60

Query: 61  AFVSLFFYLAIAYLPLLPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDD 120
              S F+Y+A  Y  LLP P  Y+AWPLYW  QGC LTG+WVIAHECGHHAFSDYQ +DD
Sbjct: 61  IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120

Query: 121 IVGLFFHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLI 180
            VGL FHS LLVPYFSWKYSHRRHHSNTGSLERDEVFVPK KS I WY KY NNP GR++
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 180

Query: 181 SLIGTLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLY 240
            L     LGWPLYLAFNVSGRPYD FACH+ P  PI+  RE +Q+Y+SDAGIL + F LY
Sbjct: 181 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 240

Query: 241 RIGVAKGAFWVMRIYGIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRD 300
           R   A+G   ++ +YG+PL+IVN FLVLITYLQHTHP+LPHY+SSEWDWLRGALATVDRD
Sbjct: 241 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGVLNRVFHNITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKTPVLKAIWREAK 360
           YG+LN+VFHNITDTHV HHLFSTMPHY+A EAT+A+KP+LG+YY+ D TP   A++REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 360

Query: 361 ECVYVEPDENANSDKGVFWYRKKL 376
           EC+YVEPD   +  KGV+WY  KL
Sbjct: 361 ECIYVEPDREGDK-KGVYWYNNKL 383

BLAST of Tan0009131 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 236.5 bits (602), Expect = 3.5e-62
Identity = 133/342 (38.89%), Postives = 187/342 (54.68%), Query Frame = 0

Query: 2   GVGSRMEKGVHERV-PHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYL 61
           G G R ++   ER  P A+PPF +  I+ A+P HC+ +S LRS SY++ D+  V+    L
Sbjct: 16  GAGDRKKE---ERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAVAA---L 75

Query: 62  AIAYLPLLPHPFPYIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSA 121
           AIA +    +   +  WPLYW  QG     ++V+ H+CGH +FSD  L++ +VG   HS 
Sbjct: 76  AIAAV----YVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSF 135

Query: 122 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLISLIGTLTLG 181
           +LVPY  W+ SHR HH N G +E DE +VP P+       K   +    L   +    L 
Sbjct: 136 ILVPYHGWRISHRTHHQNHGHVENDESWVPLPER----VYKKLPHSTRMLRYTVPLPMLA 195

Query: 182 WPLYLAFNVSGRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAF 241
           +PLYL +   G+       H++PY  +F   E   +  S      M   L  +    G  
Sbjct: 196 YPLYLCYRSPGKE----GSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPL 255

Query: 242 WVMRIYGIPLVIVNGFLVLITYLQH--THPALPHYESSEWDWLRGALATVDRDYGVLNRV 301
            V+++YG+P +I   +L  +TYL H      LP Y   EW +LRG L T+DRDYG+ N +
Sbjct: 256 AVLKVYGVPYIIFVMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNNI 315

Query: 302 FHNITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDKT 341
            H+I  THV+HHLF  +PHYH  +AT+A K VLG YYR  KT
Sbjct: 316 HHDI-GTHVIHHLFPQIPHYHLVDATKAAKHVLGRYYREPKT 338

BLAST of Tan0009131 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 229.9 bits (585), Expect = 3.3e-60
Identity = 143/371 (38.54%), Postives = 201/371 (54.18%), Query Frame = 0

Query: 16  PHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLA--IAYLPLLPHPFP 75
           P A PPF L+ I+ A+P HC+ ++  +S SY++ D+A V   F LA   AYL        
Sbjct: 90  PGAPPPFNLADIRAAIPKHCWVKNPWKSLSYVVRDVAIV---FALAAGAAYLN------N 149

Query: 76  YIAWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYFSWKYSHR 135
           +I WPLYW+ QG     ++V+ H+CGH +FS+   ++ +VG   HS++LVPY  W+ SHR
Sbjct: 150 WIVWPLYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHR 209

Query: 136 RHHSNTGSLERDEVFVPKPKSQIPWYMKYFN--NPPGRLISL-IGTLTLGWPLYLAFNVS 195
            HH N G +E DE + P  +       K +N  + P R     +  + L +P YL     
Sbjct: 210 THHQNHGHVENDESWHPMSE-------KIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSP 269

Query: 196 GRPYDRFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAFWVMRIYGIPL 255
           G+       HY P   +F  +E   V  S A    MA +L  +    G   ++++YGIP 
Sbjct: 270 GKK----GSHYHPDSDLFLPKERKDVLTSTACWTAMAALLVCLNFTIGPIQMLKLYGIPY 329

Query: 256 VIVNGFLVLITYLQH--THPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTHVV 315
            I   +L  +TYL H      LP Y   EW +LRG L T+DRDYG++N + H+I  THV+
Sbjct: 330 WINVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGLINNIHHDI-GTHVI 389

Query: 316 HHLFSTMPHYHAKEATEAVKPVLGEYYR-LDKT-----PVLKAIWREAKECVYVEPDENA 374
           HHLF  +PHYH  EATEA KPVLG+YYR  DK+      +L+ + +  KE  YV      
Sbjct: 390 HHLFPQIPHYHLVEATEAAKPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYV------ 432

BLAST of Tan0009131 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 223.0 bits (567), Expect = 4.0e-58
Identity = 126/326 (38.65%), Postives = 175/326 (53.68%), Query Frame = 0

Query: 16  PHAKPPFTLSQIKKAVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYLPLLPHPFPYI 75
           P A PPF L+ I+ A+P HC+ ++   S SY++ D+A V  F   A+A      +   ++
Sbjct: 83  PGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIV--FGLAAVA-----AYFNNWL 142

Query: 76  AWPLYWIFQGCTLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYFSWKYSHRRH 135
            WPLYW  QG     ++V+ H+CGH +FS+   ++ + G   HS++LVPY  W+ SHR H
Sbjct: 143 LWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTH 202

Query: 136 HSNTGSLERDEVFVPKPKSQIPWYMKYFNNPPGRLISLIGTLTLGWPLYLAFNVSGRPYD 195
           H N G +E DE + P P+S      K            +    L +P YL     G+   
Sbjct: 203 HQNHGHVENDESWHPLPES----IYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQ-- 262

Query: 196 RFACHYDPYGPIFTKREWIQVYISDAGILGMAFVLYRIGVAKGAFWVMRIYGIPLVIVNG 255
               HY P   +F  +E   V  S A    MA +L  +    G   ++++YGIP  I   
Sbjct: 263 --GSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKLYGIPYWIFVM 322

Query: 256 FLVLITYLQH--THPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTHVVHHLFS 315
           +L  +TYL H      LP Y   EW +LRG L T+DRDYG +N + H+I  THV+HHLF 
Sbjct: 323 WLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDI-GTHVIHHLFP 382

Query: 316 TMPHYHAKEATEAVKPVLGEYYRLDK 340
            +PHYH  EATEA KPVLG+YYR  K
Sbjct: 383 QIPHYHLVEATEAAKPVLGKYYREPK 392